AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0018720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0018720 MtrunA17_Chr1g0170581 90.984 244 22 0 1 244 1 244 1.41e-164 454
Msa0018720 MtrunA17_Chr1g0170571 87.952 249 22 1 1 249 1 241 2.34e-153 426
Msa0018720 MtrunA17_Chr1g0170591 82.329 249 36 1 1 249 1 241 4.99e-151 420
Msa0018720 MtrunA17_Chr7g0256671 58.964 251 84 7 1 241 1 242 1.07e-67 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0018720 AT3G56970.1 40.625 256 127 5 1 240 1 247 1.80e-55 178
Msa0018720 AT3G56980.1 40.304 263 127 6 1 242 1 254 1.43e-45 153
Msa0018720 AT2G41240.1 38.095 252 132 5 1 242 1 238 1.63e-45 152
Msa0018720 AT2G41240.2 37.698 252 132 6 1 242 1 237 5.19e-43 146
Msa0018720 AT5G04150.1 44.809 183 93 4 60 237 78 257 7.13e-37 131
Msa0018720 AT5G04150.2 46.012 163 83 2 60 219 78 238 1.59e-36 129

Find 52 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACCTGTTTCTGTAATTC+TGG 0.155913 1_1:-31710544 None:intergenic
TGATGCTGGTTATGGGTTAT+TGG 0.230970 1_1:-31710166 None:intergenic
TGTAACTATGCTTCTTCCTT+TGG 0.251062 1_1:-31710645 None:intergenic
CTTCTATAATTTGCATTTCC+AGG 0.251812 1_1:+31710837 Msa0018720:CDS
GAGCTTCTCATTTAGAATTT+CGG 0.255939 1_1:-31713853 None:intergenic
TTCTGAGATCTTGATATGTT+TGG 0.287728 1_1:+31710756 Msa0018720:CDS
GTAACTATGCTTCTTCCTTT+GGG 0.307422 1_1:-31710644 None:intergenic
TGAGAAGCTCTTATCAATAA+TGG 0.319674 1_1:+31713866 Msa0018720:CDS
CTTTCTTCATCTCAGACCTT+TGG 0.339976 1_1:+31710805 Msa0018720:CDS
ATGAACTCTGATGCTGGTTA+TGG 0.359283 1_1:-31710174 None:intergenic
AGGACTCTTGAAATTGTTGC+TGG 0.367920 1_1:-31710511 None:intergenic
GATCGCGTTCACTAGCATTA+TGG 0.375108 1_1:-31710315 None:intergenic
TAGAATAACCTCTTCTACTT+GGG 0.395863 1_1:-31710611 None:intergenic
TCTTCATCTCAGACCTTTGG+AGG 0.399010 1_1:+31710808 Msa0018720:CDS
GTAACCATTTGATCTGAAAC+AGG 0.410609 1_1:-31710385 None:intergenic
CATACCAGAATTACAGAAAC+AGG 0.417584 1_1:+31710540 Msa0018720:CDS
TCTTCCTGTTTCAGATCAAA+TGG 0.432892 1_1:+31710381 Msa0018720:CDS
GCTCTTATCAATAATGGATA+AGG 0.434619 1_1:+31713872 Msa0018720:CDS
ATCTCAGACCTTTGGAGGGA+GGG 0.436300 1_1:+31710813 Msa0018720:CDS
TTAGAATAACCTCTTCTACT+TGG 0.448901 1_1:-31710612 None:intergenic
ATGGGTTATTGGCTCCTCAA+AGG 0.463079 1_1:-31710155 None:intergenic
AACAATTGGATGGCCCTTTG+AGG 0.464251 1_1:+31710141 Msa0018720:CDS
TGAACTCTGATGCTGGTTAT+GGG 0.477659 1_1:-31710173 None:intergenic
TGGGTTATTGGCTCCTCAAA+GGG 0.488564 1_1:-31710154 None:intergenic
CATATCAAGATCTCAGAAAG+TGG 0.493296 1_1:-31710751 None:intergenic
CCATCCAATTGTTGAAAACA+TGG 0.496261 1_1:-31710131 None:intergenic
CATGCCAAGGTCACTGCTTG+TGG 0.499479 1_1:-31710278 None:intergenic
CATCTCAGACCTTTGGAGGG+AGG 0.501008 1_1:+31710812 Msa0018720:CDS
AAGAAAGATTAAGAAGCAAA+AGG 0.505181 1_1:-31710789 None:intergenic
CTTCATCTCAGACCTTTGGA+GGG 0.506754 1_1:+31710809 Msa0018720:CDS
ACAAATTATTGATCTTCTTG+CGG 0.529574 1_1:-31710339 None:intergenic
TTGATATGTTTGGAAAATGA+TGG 0.537710 1_1:+31710766 Msa0018720:CDS
AAGAAGCATAGTTACAACTC+TGG 0.551749 1_1:+31710652 Msa0018720:CDS
ACTTTCTCCCCAAGTAGAAG+AGG 0.556979 1_1:+31710603 Msa0018720:CDS
TGTAGAATGAACTCTGATGC+TGG 0.560692 1_1:-31710180 None:intergenic
TTATCAATAATGGATAAGGA+AGG 0.562276 1_1:+31713876 Msa0018720:CDS
TCTCAGAAAGTGGAATCTTG+TGG 0.569417 1_1:-31710741 None:intergenic
CAATCCACAAGCAGTGACCT+TGG 0.569748 1_1:+31710274 Msa0018720:CDS
AGTGTAACATGATATCTGAA+TGG 0.570967 1_1:-31710716 None:intergenic
AGAATAACCTCTTCTACTTG+GGG 0.586846 1_1:-31710610 None:intergenic
ACTAGTTAAGAGAAAAGAAG+AGG 0.589176 1_1:+31710570 Msa0018720:CDS
GTCACTGCTTGTGGATTGTG+AGG 0.591686 1_1:-31710269 None:intergenic
ATAGAAGACCCTCCCTCCAA+AGG 0.594272 1_1:-31710821 None:intergenic
TATCAATAATGGATAAGGAA+GGG 0.600012 1_1:+31713877 Msa0018720:CDS
CACAAGCAGTGACCTTGGCA+TGG 0.602895 1_1:+31710279 Msa0018720:CDS
TTCTAAGCAAAGAATCCCAA+AGG 0.620328 1_1:+31710629 Msa0018720:CDS
CATCCAATTGTTGAAAACAT+GGG 0.623866 1_1:-31710130 None:intergenic
CAAGCTTCTTAACCATGCCA+AGG 0.655760 1_1:-31710291 None:intergenic
ACCAGAATTACAGAAACAGG+TGG 0.656125 1_1:+31710543 Msa0018720:CDS
GAATTACAGAAACAGGTGGA+AGG 0.659206 1_1:+31710547 Msa0018720:CDS
CCAATTGTTGAAAACATGGG+AGG 0.674806 1_1:-31710127 None:intergenic
TCACTGCTTGTGGATTGTGA+GGG 0.698792 1_1:-31710268 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATCATTTGTATAAT+GGG - chr1_1:31711476-31711495 None:intergenic 10.0%
!! AAAAAAAATCATTTGTATAA+TGG - chr1_1:31711477-31711496 None:intergenic 10.0%
!!! GTTTTTTTGAATTTATTTAA+CGG - chr1_1:31712454-31712473 None:intergenic 10.0%
!!! GTTTTTTTGAATTTATTTAA+CGG + chr1_1:31712454-31712473 Msa0018720:intron 10.0%
!!! ACTAAAAAAAAAAATTGTTT+TGG - chr1_1:31712518-31712537 None:intergenic 10.0%
!!! ACAATTTTTTTTTTTAGTTA+AGG + chr1_1:31712520-31712539 Msa0018720:intron 10.0%
!!! CAATTTTTTTTTTTAGTTAA+GGG + chr1_1:31712521-31712540 Msa0018720:intron 10.0%
!! AGTATAAAATTTCATTTAAT+AGG + chr1_1:31713167-31713186 Msa0018720:intron 10.0%
!! GTATAAAATTTCATTTAATA+GGG + chr1_1:31713168-31713187 Msa0018720:intron 10.0%
!! TATAAAATTTCATTTAATAG+GGG + chr1_1:31713169-31713188 Msa0018720:intron 10.0%
!! ATTAACAAAATCATAAAGAA+GGG - chr1_1:31710420-31710439 None:intergenic 15.0%
!! AATTAACAAAATCATAAAGA+AGG - chr1_1:31710421-31710440 None:intergenic 15.0%
!! TTTGTCATTGTAATATTTAT+AGG - chr1_1:31711396-31711415 None:intergenic 15.0%
!! CAAGAAAAAAAGAAAAAAAA+AGG + chr1_1:31711518-31711537 Msa0018720:intron 15.0%
!!! ACAATCTTTTTTTTAGTTAA+GGG - chr1_1:31711593-31711612 None:intergenic 15.0%
!!! AACAATCTTTTTTTTAGTTA+AGG - chr1_1:31711594-31711613 None:intergenic 15.0%
!!! AACTAAAAAAAAGATTGTTT+TGG + chr1_1:31711595-31711614 Msa0018720:intron 15.0%
!! AATAAATTCAAAAAAACGTT+AGG + chr1_1:31711662-31711681 Msa0018720:intron 15.0%
!! ATAAATTCAAAAAAACGTTA+GGG + chr1_1:31711663-31711682 Msa0018720:intron 15.0%
!! GATACTAAATAAATACTTAA+GGG + chr1_1:31711738-31711757 Msa0018720:intron 15.0%
!!! AAAAAACTTAAATGTTCTTT+TGG - chr1_1:31711765-31711784 None:intergenic 15.0%
!!! TTGTCTTTTAAGTATTTATT+TGG + chr1_1:31712368-31712387 Msa0018720:intron 15.0%
!! ATAAATTCAAAAAAACGTTA+GGG - chr1_1:31711663-31711682 None:intergenic 15.0%
!! AATAAATTCAAAAAAACGTT+AGG - chr1_1:31711662-31711681 None:intergenic 15.0%
!! GTCTTTATATTTAAAAAGTA+TGG + chr1_1:31713066-31713085 Msa0018720:intron 15.0%
!! TGATTAATCATTTGAATAAT+GGG - chr1_1:31713606-31713625 None:intergenic 15.0%
!! AAACAATGAAACAAAAATGT+AGG + chr1_1:31711431-31711450 Msa0018720:intron 20.0%
!!! AATGATTTTTTTTGATGAAG+AGG + chr1_1:31711484-31711503 Msa0018720:intron 20.0%
!! GATACCAAATATATAGTTAA+GGG - chr1_1:31711562-31711581 None:intergenic 20.0%
!! TTTGAATTTATTTAACGGAA+GGG - chr1_1:31712459-31712478 None:intergenic 20.0%
!!! TTTTGAATTTATTTAACGGA+AGG - chr1_1:31712458-31712477 None:intergenic 20.0%
!!! GTATTTATTTAGTATCGTTT+TGG - chr1_1:31711734-31711753 None:intergenic 20.0%
!! CGATACTAAATAAATACTTA+AGG + chr1_1:31711737-31711756 Msa0018720:intron 20.0%
!!! TGTTATTGATTGTTATTGTT+GGG - chr1_1:31711871-31711890 None:intergenic 20.0%
!!! ATGTTATTGATTGTTATTGT+TGG - chr1_1:31711872-31711891 None:intergenic 20.0%
!! AAAAGACAAAAAGAACATTT+AGG - chr1_1:31712357-31712376 None:intergenic 20.0%
!!! TTTTGAATTTATTTAACGGA+AGG + chr1_1:31712458-31712477 Msa0018720:intron 20.0%
!! TTTGAATTTATTTAACGGAA+GGG + chr1_1:31712459-31712478 Msa0018720:intron 20.0%
!! ATAATAACATCATTATCGAT+TGG + chr1_1:31712961-31712980 Msa0018720:intron 20.0%
!!! ACTTTAATTTGTAGATGTTA+AGG - chr1_1:31713034-31713053 None:intergenic 20.0%
!! CAAAAATTTAACAAATAACG+CGG + chr1_1:31713230-31713249 Msa0018720:intron 20.0%
!! TTTATTAAAATCAATGTGGA+CGG - chr1_1:31713512-31713531 None:intergenic 20.0%
!! GTGATTAATCATTTGAATAA+TGG - chr1_1:31713607-31713626 None:intergenic 20.0%
!!! TATTAATTGATAGTTCCTTT+TGG + chr1_1:31713794-31713813 Msa0018720:intron 20.0%
!!! ATTAATTGATAGTTCCTTTT+GGG + chr1_1:31713795-31713814 Msa0018720:intron 20.0%
!!! TAATTTTCATGATCAAGTTG+AGG + chr1_1:31710225-31710244 Msa0018720:CDS 25.0%
! ACAAATTATTGATCTTCTTG+CGG - chr1_1:31710342-31710361 None:intergenic 25.0%
!! GTAATTCTGGTATGTATTTT+AGG - chr1_1:31710534-31710553 None:intergenic 25.0%
! TTGATATGTTTGGAAAATGA+TGG + chr1_1:31710766-31710785 Msa0018720:CDS 25.0%
! AAGAAAGATTAAGAAGCAAA+AGG - chr1_1:31710792-31710811 None:intergenic 25.0%
! AAGACTTTACTCAACAATAT+TGG - chr1_1:31710907-31710926 None:intergenic 25.0%
! CGATACCAAATATATAGTTA+AGG - chr1_1:31712551-31712570 None:intergenic 25.0%
!! ATGCTTTTAGATCCAAAAAT+GGG - chr1_1:31711958-31711977 None:intergenic 25.0%
!! TATGCTTTTAGATCCAAAAA+TGG - chr1_1:31711959-31711978 None:intergenic 25.0%
!!! TGAATTCTGTGTTTTTTTTC+TGG + chr1_1:31712277-31712296 Msa0018720:intron 25.0%
!! GTATTTATTTGGTATCGTTT+TGG + chr1_1:31712379-31712398 Msa0018720:intron 25.0%
! CGATACCAAATATATAGTTA+GGG + chr1_1:31712551-31712570 Msa0018720:intron 25.0%
! GATACCAAATATATAGTTAG+GGG + chr1_1:31712552-31712571 Msa0018720:intron 25.0%
!!! AGGCTTTATTAAAATCAATG+TGG - chr1_1:31713516-31713535 None:intergenic 25.0%
!!! TTGATTTTAATAAAGCCTTC+CGG + chr1_1:31713518-31713537 Msa0018720:intron 25.0%
! TCACTTGACATTTATTTGTT+GGG + chr1_1:31713567-31713586 Msa0018720:intron 25.0%
!!! TTATCAATAATGGATAAGGA+AGG + chr1_1:31713876-31713895 Msa0018720:CDS 25.0%
!!! TATCAATAATGGATAAGGAA+GGG + chr1_1:31713877-31713896 Msa0018720:CDS 25.0%
CATCCAATTGTTGAAAACAT+GGG - chr1_1:31710133-31710152 None:intergenic 30.0%
ACTAGTTAAGAGAAAAGAAG+AGG + chr1_1:31710570-31710589 Msa0018720:CDS 30.0%
TAGAATAACCTCTTCTACTT+GGG - chr1_1:31710614-31710633 None:intergenic 30.0%
TTAGAATAACCTCTTCTACT+TGG - chr1_1:31710615-31710634 None:intergenic 30.0%
! GTTTTGTAGTTTCAACAAGT+AGG + chr1_1:31710674-31710693 Msa0018720:CDS 30.0%
AGTGTAACATGATATCTGAA+TGG - chr1_1:31710719-31710738 None:intergenic 30.0%
!! TTCTGAGATCTTGATATGTT+TGG + chr1_1:31710756-31710775 Msa0018720:CDS 30.0%
! CTTCTATAATTTGCATTTCC+AGG + chr1_1:31710837-31710856 Msa0018720:CDS 30.0%
TTTATCTATGACCAATGCAT+TGG + chr1_1:31711358-31711377 Msa0018720:intron 30.0%
ACAAAAAATAGCCAATGCAT+TGG - chr1_1:31711372-31711391 None:intergenic 30.0%
! AAAAGGCTTAAATGCTCTTT+TGG + chr1_1:31711535-31711554 Msa0018720:intron 30.0%
TGGTCCCTTAACTATATATT+TGG + chr1_1:31711555-31711574 Msa0018720:intron 30.0%
CTATATATTTGGTATCGCTT+TGG + chr1_1:31711566-31711585 Msa0018720:intron 30.0%
! TTGTTGTAAGTTGTTATTGC+TGG - chr1_1:31711822-31711841 None:intergenic 30.0%
ATCAATAACAACAACAACAC+AGG + chr1_1:31711907-31711926 Msa0018720:intron 30.0%
! CAAAAAAACCAACCCATTTT+TGG + chr1_1:31711943-31711962 Msa0018720:intron 30.0%
! TTTTAGATCCAAAAATGGGT+TGG - chr1_1:31711954-31711973 None:intergenic 30.0%
!!! TTCTATTGCTGCAGTTTTTT+CGG + chr1_1:31712026-31712045 Msa0018720:intron 30.0%
CTATATATTTGGTATCGCTT+TGG - chr1_1:31711566-31711585 None:intergenic 30.0%
GCGATACCAAATATATAGTT+AGG + chr1_1:31712550-31712569 Msa0018720:intron 30.0%
!!! GATCCAGAAATTTTGACTTA+CGG + chr1_1:31712878-31712897 Msa0018720:intron 30.0%
!!! ATCCAGAAATTTTGACTTAC+GGG + chr1_1:31712879-31712898 Msa0018720:intron 30.0%
!!! GGGCAATCTATTATTTTAAG+AGG + chr1_1:31713189-31713208 Msa0018720:intron 30.0%
TGATTACAGATCTATCATCA+TGG - chr1_1:31713331-31713350 None:intergenic 30.0%
GAACTTTCAGCTATTTGAAA+GGG - chr1_1:31713375-31713394 None:intergenic 30.0%
CTTTCAAATAGCTGAAAGTT+CGG + chr1_1:31713374-31713393 Msa0018720:intron 30.0%
! AATTGGATTCATGGTTTTTC+TGG - chr1_1:31713428-31713447 None:intergenic 30.0%
!! AGGTCTTTTGAGAAACAATA+GGG - chr1_1:31713453-31713472 None:intergenic 30.0%
GTCACTTGACATTTATTTGT+TGG + chr1_1:31713566-31713585 Msa0018720:intron 30.0%
! GAGCTTCTCATTTAGAATTT+CGG - chr1_1:31713856-31713875 None:intergenic 30.0%
TGAGAAGCTCTTATCAATAA+TGG + chr1_1:31713866-31713885 Msa0018720:CDS 30.0%
!! GCTCTTATCAATAATGGATA+AGG + chr1_1:31713872-31713891 Msa0018720:CDS 30.0%
CCATCCAATTGTTGAAAACA+TGG - chr1_1:31710134-31710153 None:intergenic 35.0%
! CCATGTTTTCAACAATTGGA+TGG + chr1_1:31710131-31710150 Msa0018720:CDS 35.0%
TCTTCCTGTTTCAGATCAAA+TGG + chr1_1:31710381-31710400 Msa0018720:CDS 35.0%
! GTAACCATTTGATCTGAAAC+AGG - chr1_1:31710388-31710407 None:intergenic 35.0%
CATACCAGAATTACAGAAAC+AGG + chr1_1:31710540-31710559 Msa0018720:CDS 35.0%
AGAATAACCTCTTCTACTTG+GGG - chr1_1:31710613-31710632 None:intergenic 35.0%
TTCTAAGCAAAGAATCCCAA+AGG + chr1_1:31710629-31710648 Msa0018720:CDS 35.0%
GTAACTATGCTTCTTCCTTT+GGG - chr1_1:31710647-31710666 None:intergenic 35.0%
TGTAACTATGCTTCTTCCTT+TGG - chr1_1:31710648-31710667 None:intergenic 35.0%
! AAGAAGCATAGTTACAACTC+TGG + chr1_1:31710652-31710671 Msa0018720:CDS 35.0%
CATATCAAGATCTCAGAAAG+TGG - chr1_1:31710754-31710773 None:intergenic 35.0%
! TCCAGGTGTGTTAATTTTAC+AGG + chr1_1:31710854-31710873 Msa0018720:intron 35.0%
TCCTGTAAAATTAACACACC+TGG - chr1_1:31710858-31710877 None:intergenic 35.0%
! TTTATCTCCGTTACCTGTTT+TGG + chr1_1:31711627-31711646 Msa0018720:intron 35.0%
TGGTCCCCTAACTATATATT+TGG - chr1_1:31712559-31712578 None:intergenic 35.0%
!!! TTTTGGCTTGAATGCTCTTT+TGG - chr1_1:31712579-31712598 None:intergenic 35.0%
! AAGAGAATAAATGTGCTGAC+TGG - chr1_1:31712726-31712745 None:intergenic 35.0%
CAACTTTGCTTTCTCTCATT+AGG - chr1_1:31712798-31712817 None:intergenic 35.0%
!!! TCCAGAAATTTTGACTTACG+GGG + chr1_1:31712880-31712899 Msa0018720:intron 35.0%
AAATAACGCGGAGAAATTAG+TGG + chr1_1:31713242-31713261 Msa0018720:intron 35.0%
AATAACGCGGAGAAATTAGT+GGG + chr1_1:31713243-31713262 Msa0018720:intron 35.0%
TATTTGAAAGGGTCTTCAGT+TGG - chr1_1:31713364-31713383 None:intergenic 35.0%
CGAACTTTCAGCTATTTGAA+AGG - chr1_1:31713376-31713395 None:intergenic 35.0%
!! GTCTTTTGAGAAACAATAGG+GGG - chr1_1:31713451-31713470 None:intergenic 35.0%
!! GGTCTTTTGAGAAACAATAG+GGG - chr1_1:31713452-31713471 None:intergenic 35.0%
!! GAGGTCTTTTGAGAAACAAT+AGG - chr1_1:31713454-31713473 None:intergenic 35.0%
CCAATTGTTGAAAACATGGG+AGG - chr1_1:31710130-31710149 None:intergenic 40.0%
! CCTCCCATGTTTTCAACAAT+TGG + chr1_1:31710127-31710146 Msa0018720:CDS 40.0%
! TGATGCTGGTTATGGGTTAT+TGG - chr1_1:31710169-31710188 None:intergenic 40.0%
! TGAACTCTGATGCTGGTTAT+GGG - chr1_1:31710176-31710195 None:intergenic 40.0%
! ATGAACTCTGATGCTGGTTA+TGG - chr1_1:31710177-31710196 None:intergenic 40.0%
TGTAGAATGAACTCTGATGC+TGG - chr1_1:31710183-31710202 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG - chr1_1:31710514-31710533 None:intergenic 40.0%
ACCAGAATTACAGAAACAGG+TGG + chr1_1:31710543-31710562 Msa0018720:CDS 40.0%
!! TCCACCTGTTTCTGTAATTC+TGG - chr1_1:31710547-31710566 None:intergenic 40.0%
GAATTACAGAAACAGGTGGA+AGG + chr1_1:31710547-31710566 Msa0018720:CDS 40.0%
TCTCAGAAAGTGGAATCTTG+TGG - chr1_1:31710744-31710763 None:intergenic 40.0%
CTTTCTTCATCTCAGACCTT+TGG + chr1_1:31710805-31710824 Msa0018720:CDS 40.0%
! AACACACCAACCCAGATTTT+GGG + chr1_1:31711707-31711726 Msa0018720:intron 40.0%
!! TCGTTTTGGTCCCAAAATCT+GGG - chr1_1:31712393-31712412 None:intergenic 40.0%
!! ATCGTTTTGGTCCCAAAATC+TGG - chr1_1:31712392-31712411 None:intergenic 40.0%
! TTGCTGGTGATGAATGATGA+AGG - chr1_1:31711806-31711825 None:intergenic 40.0%
! TGGGTTGTTGTTATTGTTGC+TGG - chr1_1:31711852-31711871 None:intergenic 40.0%
!!! TTTTTTCGGTACTGTGGATG+AGG + chr1_1:31712040-31712059 Msa0018720:intron 40.0%
GGGGAAACGAAAATGAAGAT+GGG + chr1_1:31712168-31712187 Msa0018720:intron 40.0%
GGGAAACGAAAATGAAGATG+GGG + chr1_1:31712169-31712188 Msa0018720:intron 40.0%
!! ATCGTTTTGGTCCCAAAATC+TGG + chr1_1:31712392-31712411 Msa0018720:intron 40.0%
!! TCGTTTTGGTCCCAAAATCT+GGG + chr1_1:31712393-31712412 Msa0018720:intron 40.0%
TTAACGGAAGGGACTAAAAC+AGG + chr1_1:31712470-31712489 Msa0018720:intron 40.0%
GAAGGGACTAAAACAGGTAA+CGG + chr1_1:31712476-31712495 Msa0018720:intron 40.0%
AGCAGGATGTTATCCAATTG+AGG + chr1_1:31712643-31712662 Msa0018720:intron 40.0%
AGGATGTTATCCAATTGAGG+AGG + chr1_1:31712646-31712665 Msa0018720:intron 40.0%
ATGTTATCCAATTGAGGAGG+AGG + chr1_1:31712649-31712668 Msa0018720:intron 40.0%
AGGAGAATTCCACATCTTCA+AGG + chr1_1:31712669-31712688 Msa0018720:intron 40.0%
!! ATACTGACTCCTTGAAGATG+TGG - chr1_1:31712681-31712700 None:intergenic 40.0%
!! GAATAAATGTGCTGACTGGT+TGG - chr1_1:31712722-31712741 None:intergenic 40.0%
GAAGCTTTCTTCTCTTCCAT+GGG - chr1_1:31712776-31712795 None:intergenic 40.0%
AGAGAAAGCAAAGTTGCATG+AGG + chr1_1:31712802-31712821 Msa0018720:intron 40.0%
!!! CCAGAAATTTTGACTTACGG+GGG + chr1_1:31712881-31712900 Msa0018720:intron 40.0%
ATAACGCGGAGAAATTAGTG+GGG + chr1_1:31713244-31713263 Msa0018720:intron 40.0%
GTGCAGAGCTACGAATTTAT+TGG - chr1_1:31713400-31713419 None:intergenic 40.0%
AATAGGGGGAATTGGATTCA+TGG - chr1_1:31713437-31713456 None:intergenic 40.0%
TGAGAAACAATAGGGGGAAT+TGG - chr1_1:31713445-31713464 None:intergenic 40.0%
!! AATCCTGGTAATGCAGGTTT+TGG - chr1_1:31713476-31713495 None:intergenic 40.0%
! CTTGATAATCCTGGTAATGC+AGG - chr1_1:31713482-31713501 None:intergenic 40.0%
! GGATCTTCTCTTGATAATCC+TGG - chr1_1:31713491-31713510 None:intergenic 40.0%
TTAATAAAGCCTTCCGGTAG+AGG + chr1_1:31713524-31713543 Msa0018720:intron 40.0%
!! GAATAATGGGTTCATGCTCT+TGG - chr1_1:31713593-31713612 None:intergenic 40.0%
TATTTATGTGCCAGTACTCC+AGG + chr1_1:31713741-31713760 Msa0018720:intron 40.0%
ATTTATGTGCCAGTACTCCA+GGG + chr1_1:31713742-31713761 Msa0018720:intron 40.0%
!!! GTTCCTTTTGGGTTTTTTGC+AGG + chr1_1:31713806-31713825 Msa0018720:intron 40.0%
CTACCTGCAAAAAACCCAAA+AGG - chr1_1:31713812-31713831 None:intergenic 40.0%
AACAATTGGATGGCCCTTTG+AGG + chr1_1:31710141-31710160 Msa0018720:CDS 45.0%
!! TGGGTTATTGGCTCCTCAAA+GGG - chr1_1:31710157-31710176 None:intergenic 45.0%
!! ATGGGTTATTGGCTCCTCAA+AGG - chr1_1:31710158-31710177 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG - chr1_1:31710271-31710290 None:intergenic 45.0%
CAAGCTTCTTAACCATGCCA+AGG - chr1_1:31710294-31710313 None:intergenic 45.0%
GATCGCGTTCACTAGCATTA+TGG - chr1_1:31710318-31710337 None:intergenic 45.0%
ACTTTCTCCCCAAGTAGAAG+AGG + chr1_1:31710603-31710622 Msa0018720:CDS 45.0%
TCTTCATCTCAGACCTTTGG+AGG + chr1_1:31710808-31710827 Msa0018720:CDS 45.0%
CTTCATCTCAGACCTTTGGA+GGG + chr1_1:31710809-31710828 Msa0018720:CDS 45.0%
GAAGGGACCAAAACAGGTAA+CGG - chr1_1:31711637-31711656 None:intergenic 45.0%
TTAACGGAAGGGACCAAAAC+AGG - chr1_1:31711643-31711662 None:intergenic 45.0%
! CAACACACCAACCCAGATTT+TGG + chr1_1:31711706-31711725 Msa0018720:intron 45.0%
TTTGGTCCCAAAATCTGGGT+TGG - chr1_1:31712397-31712416 None:intergenic 45.0%
AAAGAACAGCTGCACAGAGA+TGG - chr1_1:31712004-31712023 None:intergenic 45.0%
!!! CTGCAGTTTTTTCGGTACTG+TGG + chr1_1:31712034-31712053 Msa0018720:intron 45.0%
GGGGGAAACGAAAATGAAGA+TGG + chr1_1:31712167-31712186 Msa0018720:intron 45.0%
TTTGGTCCCAAAATCTGGGT+TGG + chr1_1:31712397-31712416 Msa0018720:intron 45.0%
TTGGTCCCAAAATCTGGGTT+GGG + chr1_1:31712398-31712417 Msa0018720:intron 45.0%
! AACACCCCAACCCAGATTTT+GGG - chr1_1:31712406-31712425 None:intergenic 45.0%
!!! GCGGATCGGCTAGTTTTTTT+TGG - chr1_1:31712596-31712615 None:intergenic 45.0%
GAATTCTCCTCCTCCTCAAT+TGG - chr1_1:31712659-31712678 None:intergenic 45.0%
! GCTGACTGGTTGGCTAAATT+TGG - chr1_1:31712712-31712731 None:intergenic 45.0%
TGACTAAATGAGACCACCCA+TGG + chr1_1:31712757-31712776 Msa0018720:intron 45.0%
GGAAGCTTTCTTCTCTTCCA+TGG - chr1_1:31712777-31712796 None:intergenic 45.0%
AGATCCAAAGCAGTCAGTGA+CGG + chr1_1:31712856-31712875 Msa0018720:intron 45.0%
CCCCCGTAAGTCAAAATTTC+TGG - chr1_1:31712884-31712903 None:intergenic 45.0%
TAACGCGGAGAAATTAGTGG+GGG + chr1_1:31713245-31713264 Msa0018720:intron 45.0%
!! ATCCACTTGTAGTGGATTGG+AGG - chr1_1:31713277-31713296 None:intergenic 45.0%
! AAGTGACTAGTCAAGTGCGT+TGG - chr1_1:31713553-31713572 None:intergenic 45.0%
TTTATGTGCCAGTACTCCAG+GGG + chr1_1:31713743-31713762 Msa0018720:intron 45.0%
!!! TTTTTTTAAAAGAAAAATAT+TGG + chr1_1:31713677-31713696 Msa0018720:intron 5.0%
! GTCACTGCTTGTGGATTGTG+AGG - chr1_1:31710272-31710291 None:intergenic 50.0%
CAATCCACAAGCAGTGACCT+TGG + chr1_1:31710274-31710293 Msa0018720:CDS 50.0%
! ATCTCAGACCTTTGGAGGGA+GGG + chr1_1:31710813-31710832 Msa0018720:CDS 50.0%
ATAGAAGACCCTCCCTCCAA+AGG - chr1_1:31710824-31710843 None:intergenic 50.0%
TGAAGATGGGGAGAGAGAGA+TGG + chr1_1:31712181-31712200 Msa0018720:intron 50.0%
ATGGGGAGAGAGAGATGGAA+TGG + chr1_1:31712186-31712205 Msa0018720:intron 50.0%
TGGGGAGAGAGAGATGGAAT+GGG + chr1_1:31712187-31712206 Msa0018720:intron 50.0%
TGGTCCCAAAATCTGGGTTG+GGG + chr1_1:31712399-31712418 Msa0018720:intron 50.0%
! CAACACCCCAACCCAGATTT+TGG - chr1_1:31712407-31712426 None:intergenic 50.0%
!! CGAATACCTCTGGGGTGTTT+AGG - chr1_1:31712621-31712640 None:intergenic 50.0%
TCCTGCTTGCGAATACCTCT+GGG - chr1_1:31712630-31712649 None:intergenic 50.0%
ATCCTGCTTGCGAATACCTC+TGG - chr1_1:31712631-31712650 None:intergenic 50.0%
GCTTTCTTCTCTTCCATGGG+TGG - chr1_1:31712773-31712792 None:intergenic 50.0%
TGCCTCCAATCCACTACAAG+TGG + chr1_1:31713272-31713291 Msa0018720:intron 50.0%
!! CGGATCCACTTGTAGTGGAT+TGG - chr1_1:31713280-31713299 None:intergenic 50.0%
TCTCAGACTGCTTTCAGGCA+CGG - chr1_1:31713300-31713319 None:intergenic 50.0%
CTGACTCTCAGACTGCTTTC+AGG - chr1_1:31713305-31713324 None:intergenic 50.0%
AAAGGGTCTTCAGTTGGCGT+GGG - chr1_1:31713358-31713377 None:intergenic 50.0%
!! CCTGGTAATGCAGGTTTTGG+AGG - chr1_1:31713473-31713492 None:intergenic 50.0%
CCTCCAAAACCTGCATTACC+AGG + chr1_1:31713470-31713489 Msa0018720:intron 50.0%
AGTGCGTTGGAATCCTCTAC+CGG - chr1_1:31713540-31713559 None:intergenic 50.0%
CATGCCAAGGTCACTGCTTG+TGG - chr1_1:31710281-31710300 None:intergenic 55.0%
CACAAGCAGTGACCTTGGCA+TGG + chr1_1:31710279-31710298 Msa0018720:CDS 55.0%
! CATCTCAGACCTTTGGAGGG+AGG + chr1_1:31710812-31710831 Msa0018720:CDS 55.0%
ACAGCTGCACAGAGATGGAG+AGG - chr1_1:31711999-31712018 None:intergenic 55.0%
GGGGAGAGAGAGATGGAATG+GGG + chr1_1:31712188-31712207 Msa0018720:intron 55.0%
GGGAGAGAGAGATGGAATGG+GGG + chr1_1:31712189-31712208 Msa0018720:intron 55.0%
GGAGAGAGAGATGGAATGGG+GGG + chr1_1:31712190-31712209 Msa0018720:intron 55.0%
AGAGAGATGGAATGGGGGGT+AGG + chr1_1:31712194-31712213 Msa0018720:intron 55.0%
CCTGCTTGCGAATACCTCTG+GGG - chr1_1:31712629-31712648 None:intergenic 55.0%
! GGATCCGTCACTGACTGCTT+TGG - chr1_1:31712863-31712882 None:intergenic 55.0%
AGGCACGGATCCACTTGTAG+TGG - chr1_1:31713285-31713304 None:intergenic 55.0%
GAAAGGGTCTTCAGTTGGCG+TGG - chr1_1:31713359-31713378 None:intergenic 55.0%
CGTTGGAATCCTCTACCGGA+AGG - chr1_1:31713536-31713555 None:intergenic 55.0%
AGTACTCCAGGGGAGAGATG+CGG + chr1_1:31713753-31713772 Msa0018720:intron 55.0%
! GGGGTGTTTAGGCAGCGGAT+CGG - chr1_1:31712610-31712629 None:intergenic 60.0%
!! CCTCTGGGGTGTTTAGGCAG+CGG - chr1_1:31712615-31712634 None:intergenic 60.0%
!! CCCCAGAGGTATTCGCAAGC+AGG + chr1_1:31712626-31712645 Msa0018720:intron 60.0%
GCATCTCTCCCCTGGAGTAC+TGG - chr1_1:31713754-31713773 None:intergenic 60.0%
AGTACTCCGCATCTCTCCCC+TGG - chr1_1:31713762-31713781 None:intergenic 60.0%
CCGCTGCCTAAACACCCCAG+AGG + chr1_1:31712612-31712631 Msa0018720:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 31710112 31713923 31710112 ID=Msa0018720;Name=Msa0018720
chr1_1 mRNA 31710112 31713923 31710112 ID=Msa0018720-mRNA-1;Parent=Msa0018720;Name=Msa0018720-mRNA-1;_AED=0.06;_eAED=0.09;_QI=0|0|0|1|1|1|3|0|249
chr1_1 exon 31710112 31710402 31710112 ID=Msa0018720-mRNA-1:exon:10393;Parent=Msa0018720-mRNA-1
chr1_1 exon 31710496 31710858 31710496 ID=Msa0018720-mRNA-1:exon:10394;Parent=Msa0018720-mRNA-1
chr1_1 exon 31713828 31713923 31713828 ID=Msa0018720-mRNA-1:exon:10395;Parent=Msa0018720-mRNA-1
chr1_1 CDS 31710112 31710402 31710112 ID=Msa0018720-mRNA-1:cds;Parent=Msa0018720-mRNA-1
chr1_1 CDS 31710496 31710858 31710496 ID=Msa0018720-mRNA-1:cds;Parent=Msa0018720-mRNA-1
chr1_1 CDS 31713828 31713923 31713828 ID=Msa0018720-mRNA-1:cds;Parent=Msa0018720-mRNA-1
Gene Sequence

>Msa0018720

ATGTTAACGATATCTCCTCCCATGTTTTCAACAATTGGATGGCCCTTTGAGGAGCCAATAACCCATAACCAGCATCAGAGTTCATTCTACAAAGACACTGCTGATCAATTATTTAATTTTCATGATCAAGTTGAGGCAGAAATTAATTCAAAAGATCCCTCACAATCCACAAGCAGTGACCTTGGCATGGTTAAGAAGCTTGTCCATAATGCTAGTGAACGCGATCGCCGCAAGAAGATCAATAATTTGTATTCATCACTTCGTTCACTTCTTCCTGTTTCAGATCAAATGAAGAAATTGAGCATTCCAGCAACAATTTCAAGAGTCCTAAAATACATACCAGAATTACAGAAACAGGTGGAAGGACTAGTTAAGAGAAAAGAAGAGGTCTTATTGAGACTTTCTCCCCAAGTAGAAGAGGTTATTCTAAGCAAAGAATCCCAAAGGAAGAAGCATAGTTACAACTCTGGTTTTGTAGTTTCAACAAGTAGGCTCAATGATAGTGAAATTACCATTCAGATATCATGTTACACTGTCCACAAGATTCCACTTTCTGAGATCTTGATATGTTTGGAAAATGATGGCCTTTTGCTTCTTAATCTTTCTTCATCTCAGACCTTTGGAGGGAGGGTCTTCTATAATTTGCATTTCCAGGTAGATAAAACACAAAGATTAGAATCCGAAATTCTAAATGAGAAGCTCTTATCAATAATGGATAAGGAAGGGATATTTGAAGTCAACAATGATTAG
Protein sequence

>Msa0018720

MLTISPPMFSTIGWPFEEPITHNQHQSSFYKDTADQLFNFHDQVEAEINSKDPSQSTSSDLGMVKKLVHNASERDRRKKINNLYSSLRSLLPVSDQMKKLSIPATISRVLKYIPELQKQVEGLVKRKEEVLLRLSPQVEEVILSKESQRKKHSYNSGFVVSTSRLNDSEITIQISCYTVHKIPLSEILICLENDGLLLLNLSSSQTFGGRVFYNLHFQVDKTQRLESEILNEKLLSIMDKEGIFEVNND*