AlfalfaGEDB | Msa0018720

M. sativa cultivar zhongmu-4 / Msa0018720

KEGG: K18486
GO: GO:0000302 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0006357 GO:0006873 GO:0006875 GO:0006879 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010104 GO:0010106 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0019219 GO:0019222 GO:0019725 GO:0023051 GO:0030003 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034248 GO:0034250 GO:0042221 GO:0042493 GO:0042592 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0043565 GO:0044212 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0046685 GO:0046686 GO:0046916 GO:0048518 GO:0048522 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051171 GO:0051173 GO:0051193 GO:0051194 GO:0051252 GO:0051254 GO:0051716 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0060255 GO:0065007 GO:0065008 GO:0070297 GO:0071496 GO:0071731 GO:0080090 GO:0090575 GO:0097159 GO:0097366 GO:0098771 GO:0140110 GO:1900376 GO:1900378 GO:1900704 GO:1900706 GO:1901363 GO:1901698 GO:1901700 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990641 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0018720	sp\|Q9M1K1\|ORG2_ARATH	40.625	256	127	5	1	240	1	247	1.77e-54	178

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0018720	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0018720	MtrunA17_Chr1g0170581	90.984	244	22	0	1	244	1	244	1.41e-164	454
Msa0018720	MtrunA17_Chr1g0170571	87.952	249	22	1	1	249	1	241	2.34e-153	426
Msa0018720	MtrunA17_Chr1g0170591	82.329	249	36	1	1	249	1	241	4.99e-151	420
Msa0018720	MtrunA17_Chr7g0256671	58.964	251	84	7	1	241	1	242	1.07e-67	209

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0018720	AT3G56970.1	40.625	256	127	5	1	240	1	247	1.80e-55	178
Msa0018720	AT3G56980.1	40.304	263	127	6	1	242	1	254	1.43e-45	153
Msa0018720	AT2G41240.1	38.095	252	132	5	1	242	1	238	1.63e-45	152
Msa0018720	AT2G41240.2	37.698	252	132	6	1	242	1	237	5.19e-43	146
Msa0018720	AT5G04150.1	44.809	183	93	4	60	237	78	257	7.13e-37	131
Msa0018720	AT5G04150.2	46.012	163	83	2	60	219	78	238	1.59e-36	129

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Find 52 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATTGGATGGCCCTTTG+AGG	0.464251	1_1:+31710141	Msa0018720:CDS
AAGAAAGATTAAGAAGCAAA+AGG	0.505181	1_1:-31710789	None:intergenic
AAGAAGCATAGTTACAACTC+TGG	0.551749	1_1:+31710652	Msa0018720:CDS
ACAAATTATTGATCTTCTTG+CGG	0.529574	1_1:-31710339	None:intergenic
ACCAGAATTACAGAAACAGG+TGG	0.656125	1_1:+31710543	Msa0018720:CDS
ACTAGTTAAGAGAAAAGAAG+AGG	0.589176	1_1:+31710570	Msa0018720:CDS
ACTTTCTCCCCAAGTAGAAG+AGG	0.556979	1_1:+31710603	Msa0018720:CDS
AGAATAACCTCTTCTACTTG+GGG	0.586846	1_1:-31710610	None:intergenic
AGGACTCTTGAAATTGTTGC+TGG	0.367920	1_1:-31710511	None:intergenic
AGTGTAACATGATATCTGAA+TGG	0.570967	1_1:-31710716	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATCCAATTGTTGAAAACAT+GGG	-	chr1_1:31710133-31710152	None:intergenic	30.0%
ACTAGTTAAGAGAAAAGAAG+AGG	+	chr1_1:31710570-31710589	Msa0018720:CDS	30.0%
TAGAATAACCTCTTCTACTT+GGG	-	chr1_1:31710614-31710633	None:intergenic	30.0%
TTAGAATAACCTCTTCTACT+TGG	-	chr1_1:31710615-31710634	None:intergenic	30.0%
AGTGTAACATGATATCTGAA+TGG	-	chr1_1:31710719-31710738	None:intergenic	30.0%
TTTATCTATGACCAATGCAT+TGG	+	chr1_1:31711358-31711377	Msa0018720:intron	30.0%
ACAAAAAATAGCCAATGCAT+TGG	-	chr1_1:31711372-31711391	None:intergenic	30.0%
TGGTCCCTTAACTATATATT+TGG	+	chr1_1:31711555-31711574	Msa0018720:intron	30.0%
CTATATATTTGGTATCGCTT+TGG	+	chr1_1:31711566-31711585	Msa0018720:intron	30.0%
ATCAATAACAACAACAACAC+AGG	+	chr1_1:31711907-31711926	Msa0018720:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	31710112	31713923	31710112	ID=Msa0018720;Name=Msa0018720
chr1_1	mRNA	31710112	31713923	31710112	ID=Msa0018720-mRNA-1;Parent=Msa0018720;Name=Msa0018720-mRNA-1;_AED=0.06;_eAED=0.09;_QI=0\|0\|0\|1\|1\|1\|3\|0\|249
chr1_1	exon	31710112	31710402	31710112	ID=Msa0018720-mRNA-1:exon:10393;Parent=Msa0018720-mRNA-1
chr1_1	exon	31710496	31710858	31710496	ID=Msa0018720-mRNA-1:exon:10394;Parent=Msa0018720-mRNA-1
chr1_1	exon	31713828	31713923	31713828	ID=Msa0018720-mRNA-1:exon:10395;Parent=Msa0018720-mRNA-1
chr1_1	CDS	31710112	31710402	31710112	ID=Msa0018720-mRNA-1:cds;Parent=Msa0018720-mRNA-1
chr1_1	CDS	31710496	31710858	31710496	ID=Msa0018720-mRNA-1:cds;Parent=Msa0018720-mRNA-1
chr1_1	CDS	31713828	31713923	31713828	ID=Msa0018720-mRNA-1:cds;Parent=Msa0018720-mRNA-1

Gene Sequence

>Msa0018720

ATGTTAACGATATCTCCTCCCATGTTTTCAACAATTGGATGGCCCTTTGAGGAGCCAATAACCCATAACCAGCATCAGAGTTCATTCTACAAAGACACTGCTGATCAATTATTTAATTTTCATGATCAAGTTGAGGCAGAAATTAATTCAAAAGATCCCTCACAATCCACAAGCAGTGACCTTGGCATGGTTAAGAAGCTTGTCCATAATGCTAGTGAACGCGATCGCCGCAAGAAGATCAATAATTTGTATTCATCACTTCGTTCACTTCTTCCTGTTTCAGATCAAATGAAGAAATTGAGCATTCCAGCAACAATTTCAAGAGTCCTAAAATACATACCAGAATTACAGAAACAGGTGGAAGGACTAGTTAAGAGAAAAGAAGAGGTCTTATTGAGACTTTCTCCCCAAGTAGAAGAGGTTATTCTAAGCAAAGAATCCCAAAGGAAGAAGCATAGTTACAACTCTGGTTTTGTAGTTTCAACAAGTAGGCTCAATGATAGTGAAATTACCATTCAGATATCATGTTACACTGTCCACAAGATTCCACTTTCTGAGATCTTGATATGTTTGGAAAATGATGGCCTTTTGCTTCTTAATCTTTCTTCATCTCAGACCTTTGGAGGGAGGGTCTTCTATAATTTGCATTTCCAGGTAGATAAAACACAAAGATTAGAATCCGAAATTCTAAATGAGAAGCTCTTATCAATAATGGATAAGGAAGGGATATTTGAAGTCAACAATGATTAG

Protein sequence

>Msa0018720

MLTISPPMFSTIGWPFEEPITHNQHQSSFYKDTADQLFNFHDQVEAEINSKDPSQSTSSDLGMVKKLVHNASERDRRKKINNLYSSLRSLLPVSDQMKKLSIPATISRVLKYIPELQKQVEGLVKRKEEVLLRLSPQVEEVILSKESQRKKHSYNSGFVVSTSRLNDSEITIQISCYTVHKIPLSEILICLENDGLLLLNLSSSQTFGGRVFYNLHFQVDKTQRLESEILNEKLLSIMDKEGIFEVNND*