AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0020610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0020610 MtrunA17_Chr1g0164761 83.305 581 59 5 1 563 1 561 0.0 975
Msa0020610 MtrunA17_Chr1g0164731 72.348 575 113 5 2 557 39 586 0.0 820
Msa0020610 MtrunA17_Chr5g0410721 49.398 498 229 4 2 477 83 579 1.27e-172 510
Msa0020610 MtrunA17_Chr3g0116441 38.571 350 180 7 9 329 19 362 1.52e-66 235
Msa0020610 MtrunA17_Chr7g0214951 77.500 120 9 2 164 282 99 201 1.84e-56 189
Msa0020610 MtrunA17_Chr1g0199511 45.745 188 98 1 135 322 291 474 3.59e-53 195
Msa0020610 MtrunA17_Chr5g0397501 34.545 385 200 13 92 447 323 684 3.74e-51 189
Msa0020610 MtrunA17_Chr1g0189211 33.594 384 214 12 90 446 301 670 3.11e-47 177
Msa0020610 MtrunA17_Chr8g0380981 33.894 416 220 11 79 460 184 578 4.73e-47 177
Msa0020610 MtrunA17_Chr2g0303101 42.128 235 116 6 105 327 219 445 2.52e-44 169
Msa0020610 MtrunA17_Chr7g0261401 31.193 327 178 12 139 439 338 643 6.22e-30 125
Msa0020610 MtrunA17_Chr7g0214941 73.973 73 18 1 453 524 2 74 3.46e-26 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0020610 AT5G46910.2 51.717 466 207 3 7 455 102 566 5.74e-171 499
Msa0020610 AT5G46910.1 51.717 466 207 3 7 455 102 566 1.86e-169 500
Msa0020610 AT1G63490.1 36.170 376 171 11 76 414 52 395 4.82e-57 207
Msa0020610 AT1G63490.4 36.267 375 170 11 77 414 306 648 8.60e-57 206
Msa0020610 AT1G63490.2 36.267 375 170 11 77 414 306 648 8.84e-57 206
Msa0020610 AT2G34880.1 30.354 537 268 16 7 454 60 579 1.04e-55 201
Msa0020610 AT3G48430.1 34.239 368 183 7 9 322 21 383 1.05e-55 203
Msa0020610 AT5G04240.1 46.277 188 97 1 135 322 259 442 5.10e-52 192
Msa0020610 AT1G30810.3 32.530 415 230 12 73 450 179 580 7.42e-48 179
Msa0020610 AT1G30810.2 32.530 415 230 12 73 450 179 580 7.42e-48 179
Msa0020610 AT1G30810.1 32.530 415 230 12 73 450 179 580 7.42e-48 179
Msa0020610 AT4G20400.2 31.738 397 219 13 79 446 146 519 2.32e-45 172
Msa0020610 AT4G20400.1 31.738 397 219 13 79 446 203 576 2.73e-45 172
Msa0020610 AT1G08620.3 40.254 236 121 4 100 322 327 555 6.81e-45 171
Msa0020610 AT1G08620.2 40.254 236 121 4 100 322 313 541 7.56e-45 171
Msa0020610 AT1G08620.4 40.254 236 121 4 100 322 313 541 7.56e-45 171
Msa0020610 AT1G08620.1 40.254 236 121 4 100 322 313 541 7.56e-45 171
Msa0020610 AT2G38950.1 24.677 620 342 22 2 521 102 696 1.06e-31 130
Msa0020610 AT1G63490.3 34.848 198 86 5 237 414 15 189 2.53e-24 108

Find 88 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAATTATACAGCCTCTT+AGG 0.183333 1_1:+35890356 Msa0020610:CDS
GATGCATTCATTTATGTTAT+AGG 0.215836 1_1:-35892624 None:intergenic
TAGGAGAATATATTTGTTAA+AGG 0.243804 1_1:-35892605 None:intergenic
AATCCAGCATGATATGAATT+TGG 0.254423 1_1:-35891453 None:intergenic
TTCTTTACCACGCAATCTAT+TGG 0.264442 1_1:-35890565 None:intergenic
ACTGAAAACAGAATAATAAT+TGG 0.313275 1_1:+35892822 Msa0020610:intron
AGTTCCTCATATGGAATTAT+AGG 0.316333 1_1:-35892085 None:intergenic
GAGAAAGGAACAGTTGAGTA+TGG 0.324983 1_1:+35890642 Msa0020610:CDS
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1_1:-35892094 None:intergenic
ATATAGGACTCGTTGTCTTT+TGG 0.352203 1_1:-35893002 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.359872 1_1:-35892370 None:intergenic
AGCTCTTCAAATACATCATT+AGG 0.370354 1_1:+35892274 Msa0020610:CDS
AACAAGCAATTTCAATTTGA+AGG 0.380018 1_1:+35890716 Msa0020610:CDS
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1_1:+35892011 Msa0020610:CDS
TGACATGCAGCCTTTGCAAT+AGG 0.385933 1_1:+35892302 Msa0020610:CDS
CTCCATTGCGTTTAGTTGAC+AGG 0.390855 1_1:+35890923 Msa0020610:CDS
GACAAAAGAAGCAGGATTAG+AGG 0.392437 1_1:-35890303 None:intergenic
TCCTTCAATATCTTCCCGTT+TGG 0.397801 1_1:+35893063 Msa0020610:CDS
CACAATTATACAGCCTCTTA+GGG 0.413225 1_1:+35890357 Msa0020610:CDS
CTGATCCTATGCTTTACATT+GGG 0.424948 1_1:+35891047 Msa0020610:CDS
TGCTGCCAAAAGTCTTCAAC+AGG 0.428587 1_1:+35892559 Msa0020610:CDS
CTTGTTCCAAGCTTATCATC+AGG 0.440827 1_1:-35890699 None:intergenic
GACATGCAGCCTTTGCAATA+GGG 0.448222 1_1:+35892303 Msa0020610:CDS
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1_1:+35892004 Msa0020610:CDS
TTGGTGATTGGTTTCCACTC+GGG 0.456519 1_1:+35892023 Msa0020610:CDS
TTTGATTCGAAAACCCTAAG+AGG 0.457545 1_1:-35890370 None:intergenic
GCTACCTATAATTCCATATG+AGG 0.458631 1_1:+35892081 Msa0020610:CDS
GGAAGATATTGAAGGAACTT+TGG 0.460688 1_1:-35893056 None:intergenic
TTTGTCTTAACGGAAGAGAA+AGG 0.469912 1_1:+35890320 Msa0020610:CDS
TGCTCAAACTCTTGTTCTGA+TGG 0.476367 1_1:-35889806 None:intergenic
TAACTTGAGAAAACATGAAT+CGG 0.480484 1_1:+35893024 Msa0020610:CDS
TACAAGGCGGTGCAAAAGCC+AGG 0.481676 1_1:+35891411 Msa0020610:CDS
TTAAAAGAACATGGAGAGAA+TGG 0.487266 1_1:+35891315 Msa0020610:CDS
ACATAATGTGCCGGTTTACA+AGG 0.487938 1_1:+35891395 Msa0020610:CDS
ATACCAAATTTAGACGCCTC+GGG 0.488483 1_1:-35889857 None:intergenic
TTGAAGATCACTATTTGTAC+AGG 0.488775 1_1:+35891098 Msa0020610:CDS
GTGCCACAAAATTGTTTATG+TGG 0.491043 1_1:+35892488 Msa0020610:CDS
ATTTGAAGCTCTTGCAAACA+AGG 0.496352 1_1:+35890536 Msa0020610:CDS
TTTCCAAATTCATATCATGC+TGG 0.496493 1_1:+35891450 Msa0020610:CDS
TTGTCTTAACGGAAGAGAAA+GGG 0.497242 1_1:+35890321 Msa0020610:CDS
ATCATCAGGATCACTTGAAA+AGG 0.501772 1_1:-35890685 None:intergenic
GGTGCAAATAAAACTTGGTA+TGG 0.503293 1_1:+35891225 Msa0020610:CDS
ATTGGTGATTGGTTTCCACT+CGG 0.503752 1_1:+35892022 Msa0020610:CDS
TTGGTACTCATCATCACTGA+TGG 0.505321 1_1:-35892983 None:intergenic
ACGAGGTCTTACCATGGAAA+AGG 0.511206 1_1:-35892378 None:intergenic
GAAATAGTACATGGAGAGAA+AGG 0.511259 1_1:+35890627 Msa0020610:CDS
CAAAATCAAACTTGAAGATA+CGG 0.514277 1_1:+35892159 Msa0020610:CDS
ACTGATCCTATGCTTTACAT+TGG 0.515442 1_1:+35891046 Msa0020610:CDS
AGTGATCCTGATGATAAGCT+TGG 0.516084 1_1:+35890693 Msa0020610:CDS
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1_1:+35891071 Msa0020610:CDS
TGGTGATTGGTTTCCACTCG+GGG 0.521993 1_1:+35892024 Msa0020610:CDS
TCCGTTAAGACAAAAGAAGC+AGG 0.523048 1_1:-35890311 None:intergenic
GAGAACATACTGTATTTAAG+AGG 0.523058 1_1:+35892513 Msa0020610:CDS
TAAGACATCGCTATCGCAGT+TGG 0.526345 1_1:+35892231 Msa0020610:CDS
AATTGAGAGGATGCAGAACT+AGG 0.531709 1_1:-35891252 None:intergenic
ACTCAGGTGCAAATAAAACT+TGG 0.537060 1_1:+35891220 Msa0020610:CDS
TTATGTTCTAACAAGACATC+AGG 0.538881 1_1:-35891378 None:intergenic
CTTGTTAGAACATAATGTGC+CGG 0.547471 1_1:+35891386 Msa0020610:CDS
AGAAGAACTGTTAACTCGCT+AGG 0.552336 1_1:+35892863 Msa0020610:CDS
AATGGAGACTGTGGCAGCCG+CGG 0.554838 1_1:-35890907 None:intergenic
GCAGGATTAGAGGCAGCGAT+TGG 0.560174 1_1:-35890293 None:intergenic
AAGGTGACGACAAACTCTCC+TGG 0.561754 1_1:-35891429 None:intergenic
CAAGACATCAGGAGAGAACA+TGG 0.563327 1_1:-35891367 None:intergenic
CATACCAAATTTAGACGCCT+CGG 0.566207 1_1:-35889858 None:intergenic
TGTTCTGATGGATAATACGT+TGG 0.567602 1_1:-35889794 None:intergenic
TTCCTGTCAACTAAACGCAA+TGG 0.577093 1_1:-35890925 None:intergenic
GCCAAACGGGAAGATATTGA+AGG 0.578027 1_1:-35893064 None:intergenic
CATAACAATCCCTATTGCAA+AGG 0.580709 1_1:-35892312 None:intergenic
TTGCTAGCTGCGGCCCCGAG+TGG 0.581206 1_1:-35892037 None:intergenic
ATTTAAGAGGAATGACATGT+TGG 0.584034 1_1:+35892526 Msa0020610:CDS
TTCTGGCACGAAATAGTACA+TGG 0.584729 1_1:+35890618 Msa0020610:CDS
TATCATGCTGGATTTAGTCA+TGG 0.587724 1_1:+35891462 Msa0020610:CDS
TGCCACAAAATTGTTTATGT+GGG 0.596267 1_1:+35892489 Msa0020610:CDS
ATTTGTTAAAGGTAACGCGT+TGG 0.609530 1_1:-35892594 None:intergenic
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1_1:-35891052 None:intergenic
TACAACTCCTCAAAAGCTAG+AGG 0.624733 1_1:+35892133 Msa0020610:CDS
TGAATAACGTTTGCTAGCTG+CGG 0.626370 1_1:-35892047 None:intergenic
AGCATAAATTACCATCACTC+AGG 0.628951 1_1:+35891204 Msa0020610:intron
ACAGAATAATAATTGGCATG+AGG 0.629929 1_1:+35892829 Msa0020610:intron
TACCAAATTTAGACGCCTCG+GGG 0.634434 1_1:-35889856 None:intergenic
AGCATGATATGAATTTGGAA+AGG 0.636839 1_1:-35891448 None:intergenic
AACAAAGTCTTAAAAGAACA+TGG 0.646276 1_1:+35891306 Msa0020610:CDS
TGAATGCATCAAAGAACACA+AGG 0.658928 1_1:+35892637 Msa0020610:CDS
ACCAAATTTAGACGCCTCGG+GGG 0.671194 1_1:-35889855 None:intergenic
GAATCGTTGTAGAAACAATG+TGG 0.697423 1_1:+35892958 Msa0020610:CDS
ACTAAACGCAATGGAGACTG+TGG 0.698121 1_1:-35890916 None:intergenic
TAATGTGCCGGTTTACAAGG+CGG 0.701604 1_1:+35891398 Msa0020610:CDS
TGAGTATGGAGTCAATGTTG+AGG 0.703101 1_1:+35890656 Msa0020610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTATTCAAAATTTC+AGG + chr1_1:35891018-35891037 Msa0020610:intron 10.0%
!!! TAATTTTATTCAAAATTTCA+GGG + chr1_1:35891019-35891038 Msa0020610:intron 10.0%
!!! TATTGTTTTCTTTTCATTTT+AGG + chr1_1:35889896-35889915 Msa0020610:intron 15.0%
!!! ATTGTTTTCTTTTCATTTTA+GGG + chr1_1:35889897-35889916 Msa0020610:intron 15.0%
!! ATATTTGATCTTAATATTTC+AGG + chr1_1:35890251-35890270 Msa0020610:intron 15.0%
!!! AACAACTTTTAATTCATATT+AGG - chr1_1:35890979-35890998 None:intergenic 15.0%
!! AGAACGATATAATCTAATAT+AGG + chr1_1:35890159-35890178 Msa0020610:intron 20.0%
!!! TAACTTTTTCTAAGAGAAAA+AGG + chr1_1:35890407-35890426 Msa0020610:intron 20.0%
!! TGATGAAAAATTAACTAACT+TGG + chr1_1:35890473-35890492 Msa0020610:intron 20.0%
!!! CTTATATTTTACGAATAAGT+AGG + chr1_1:35891588-35891607 Msa0020610:intron 20.0%
!!! AATCTGTTTATTTTTGTACA+AGG + chr1_1:35891760-35891779 Msa0020610:intron 20.0%
!!! TTTTTTTCCATAATAATACC+TGG + chr1_1:35891840-35891859 Msa0020610:intron 20.0%
!!! ACATAAGTATTTAAAATAGC+AGG - chr1_1:35891879-35891898 None:intergenic 20.0%
!!! TAGGAGAATATATTTGTTAA+AGG - chr1_1:35892608-35892627 None:intergenic 20.0%
!! ACTATTATAATGAAGTTTAG+AGG - chr1_1:35892706-35892725 None:intergenic 20.0%
!!! ATCTGATTGATTTTAACTTT+TGG + chr1_1:35892792-35892811 Msa0020610:intron 20.0%
!! ACTGAAAACAGAATAATAAT+TGG + chr1_1:35892822-35892841 Msa0020610:intron 20.0%
!! ATTTTCTGCAGATAAACTAA+AGG - chr1_1:35889833-35889852 None:intergenic 25.0%
! AGTAGAATTCTTACTATACT+TGG - chr1_1:35889973-35889992 None:intergenic 25.0%
!! AGATTTTACTAAAATCACAG+TGG + chr1_1:35890120-35890139 Msa0020610:intron 25.0%
! AACAAGCAATTTCAATTTGA+AGG + chr1_1:35890716-35890735 Msa0020610:CDS 25.0%
! AACAAAGTCTTAAAAGAACA+TGG + chr1_1:35891306-35891325 Msa0020610:CDS 25.0%
!!! TTGTTTTTTGTGCAAGAAAT+TGG - chr1_1:35891347-35891366 None:intergenic 25.0%
!! AGGTTGATTGAATAATAGAA+AGG + chr1_1:35891612-35891631 Msa0020610:intron 25.0%
! CTTTCTATTATTCAAGCTTA+GGG - chr1_1:35891667-35891686 None:intergenic 25.0%
!! ATCATTTTCTTGTTGAGTTT+AGG + chr1_1:35891968-35891987 Msa0020610:intron 25.0%
! CAAAATCAAACTTGAAGATA+CGG + chr1_1:35892159-35892178 Msa0020610:CDS 25.0%
!! ATGGATAAACTTGCTTATTA+TGG + chr1_1:35892450-35892469 Msa0020610:intron 25.0%
! GATGCATTCATTTATGTTAT+AGG - chr1_1:35892627-35892646 None:intergenic 25.0%
!! CATGTTTTCTCAAGTTATAT+AGG - chr1_1:35893021-35893040 None:intergenic 25.0%
! TAACTTGAGAAAACATGAAT+CGG + chr1_1:35893024-35893043 Msa0020610:CDS 25.0%
AAAGAAAGTTGAGGGATAAA+AGG - chr1_1:35890007-35890026 None:intergenic 30.0%
! TTTATCCCTCAACTTTCTTT+TGG + chr1_1:35890007-35890026 Msa0020610:intron 30.0%
!!! TTTGATTTTAGAATTTGCCG+CGG + chr1_1:35890890-35890909 Msa0020610:intron 30.0%
TACAAAAATTGCTTCCTTAC+TGG - chr1_1:35890955-35890974 None:intergenic 30.0%
TTGAAGATCACTATTTGTAC+AGG + chr1_1:35891098-35891117 Msa0020610:CDS 30.0%
TTAAAAGAACATGGAGAGAA+TGG + chr1_1:35891315-35891334 Msa0020610:CDS 30.0%
TTATGTTCTAACAAGACATC+AGG - chr1_1:35891381-35891400 None:intergenic 30.0%
AGCATGATATGAATTTGGAA+AGG - chr1_1:35891451-35891470 None:intergenic 30.0%
TTTCCAAATTCATATCATGC+TGG + chr1_1:35891450-35891469 Msa0020610:CDS 30.0%
AATCCAGCATGATATGAATT+TGG - chr1_1:35891456-35891475 None:intergenic 30.0%
! TATTTTACGAATAAGTAGGC+AGG + chr1_1:35891592-35891611 Msa0020610:intron 30.0%
! TGAATAATAGAAAGGTACGT+AGG + chr1_1:35891620-35891639 Msa0020610:intron 30.0%
CCTTTCTATTATTCAAGCTT+AGG - chr1_1:35891668-35891687 None:intergenic 30.0%
CCTAAGCTTGAATAATAGAA+AGG + chr1_1:35891665-35891684 Msa0020610:intron 30.0%
! TTTTCCATAATAATACCTGG+CGG + chr1_1:35891843-35891862 Msa0020610:intron 30.0%
!!! TTGAGTTTAGGTTTTAACTG+TGG + chr1_1:35891980-35891999 Msa0020610:intron 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG + chr1_1:35892011-35892030 Msa0020610:CDS 30.0%
AGTTCCTCATATGGAATTAT+AGG - chr1_1:35892088-35892107 None:intergenic 30.0%
TTACAAAGAAGTTCCTCATA+TGG - chr1_1:35892097-35892116 None:intergenic 30.0%
AGCTCTTCAAATACATCATT+AGG + chr1_1:35892274-35892293 Msa0020610:CDS 30.0%
TGCCACAAAATTGTTTATGT+GGG + chr1_1:35892489-35892508 Msa0020610:CDS 30.0%
GAGAACATACTGTATTTAAG+AGG + chr1_1:35892513-35892532 Msa0020610:CDS 30.0%
ATTTAAGAGGAATGACATGT+TGG + chr1_1:35892526-35892545 Msa0020610:CDS 30.0%
! TTTTTCCTGTTGAAGACTTT+TGG - chr1_1:35892567-35892586 None:intergenic 30.0%
TAACACATTGCTTTATCTTC+AGG + chr1_1:35892753-35892772 Msa0020610:intron 30.0%
ACAGAATAATAATTGGCATG+AGG + chr1_1:35892829-35892848 Msa0020610:intron 30.0%
! TGTTCTGATGGATAATACGT+TGG - chr1_1:35889797-35889816 None:intergenic 35.0%
TGGATCCAAAAGAAAGTTGA+GGG - chr1_1:35890015-35890034 None:intergenic 35.0%
ATGGATCCAAAAGAAAGTTG+AGG - chr1_1:35890016-35890035 None:intergenic 35.0%
! GCAACAGATTCTGCATTTTT+AGG + chr1_1:35890194-35890213 Msa0020610:intron 35.0%
! TCCTGCTTCTTTTGTCTTAA+CGG + chr1_1:35890310-35890329 Msa0020610:CDS 35.0%
TTTGTCTTAACGGAAGAGAA+AGG + chr1_1:35890320-35890339 Msa0020610:CDS 35.0%
TTGTCTTAACGGAAGAGAAA+GGG + chr1_1:35890321-35890340 Msa0020610:CDS 35.0%
ACACAATTATACAGCCTCTT+AGG + chr1_1:35890356-35890375 Msa0020610:CDS 35.0%
CACAATTATACAGCCTCTTA+GGG + chr1_1:35890357-35890376 Msa0020610:CDS 35.0%
! TTTGATTCGAAAACCCTAAG+AGG - chr1_1:35890373-35890392 None:intergenic 35.0%
! GGTTTTCGAATCAAATGAGA+TGG + chr1_1:35890378-35890397 Msa0020610:intron 35.0%
! ATTTGAAGCTCTTGCAAACA+AGG + chr1_1:35890536-35890555 Msa0020610:CDS 35.0%
TTCTTTACCACGCAATCTAT+TGG - chr1_1:35890568-35890587 None:intergenic 35.0%
GAAATAGTACATGGAGAGAA+AGG + chr1_1:35890627-35890646 Msa0020610:CDS 35.0%
ATCATCAGGATCACTTGAAA+AGG - chr1_1:35890688-35890707 None:intergenic 35.0%
TTGACAGGAAAATTCCAGTA+AGG + chr1_1:35890938-35890957 Msa0020610:intron 35.0%
ACTGATCCTATGCTTTACAT+TGG + chr1_1:35891046-35891065 Msa0020610:CDS 35.0%
CTGATCCTATGCTTTACATT+GGG + chr1_1:35891047-35891066 Msa0020610:CDS 35.0%
TGCTATTCAGTATGTTTGCA+TGG + chr1_1:35891071-35891090 Msa0020610:CDS 35.0%
AGCATAAATTACCATCACTC+AGG + chr1_1:35891204-35891223 Msa0020610:intron 35.0%
!! TTTTATTTGCACCTGAGTGA+TGG - chr1_1:35891218-35891237 None:intergenic 35.0%
ACTCAGGTGCAAATAAAACT+TGG + chr1_1:35891220-35891239 Msa0020610:CDS 35.0%
GGTGCAAATAAAACTTGGTA+TGG + chr1_1:35891225-35891244 Msa0020610:CDS 35.0%
! GACAGTCTTTTCAAATTGAG+AGG - chr1_1:35891268-35891287 None:intergenic 35.0%
CTTGTTAGAACATAATGTGC+CGG + chr1_1:35891386-35891405 Msa0020610:CDS 35.0%
TATCATGCTGGATTTAGTCA+TGG + chr1_1:35891462-35891481 Msa0020610:CDS 35.0%
!! TGGTGTTATTACGTTTCTGA+TGG + chr1_1:35891559-35891578 Msa0020610:intron 35.0%
! AGAAAGGTACGTAGGTTTAA+AGG + chr1_1:35891628-35891647 Msa0020610:intron 35.0%
CGAGATTTGATTATGGAGTT+CGG - chr1_1:35891728-35891747 None:intergenic 35.0%
AATTCCGCCAGGTATTATTA+TGG - chr1_1:35891850-35891869 None:intergenic 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG + chr1_1:35892004-35892023 Msa0020610:CDS 35.0%
GCTACCTATAATTCCATATG+AGG + chr1_1:35892081-35892100 Msa0020610:CDS 35.0%
CATAACAATCCCTATTGCAA+AGG - chr1_1:35892315-35892334 None:intergenic 35.0%
TTACCATGGAAAAGGCAAAT+TGG - chr1_1:35892373-35892392 None:intergenic 35.0%
GAAATACTCAGAGAATAACG+AGG - chr1_1:35892398-35892417 None:intergenic 35.0%
! TAAGCTGCTTGTGTTATTGA+TGG + chr1_1:35892431-35892450 Msa0020610:intron 35.0%
GTGCCACAAAATTGTTTATG+TGG + chr1_1:35892488-35892507 Msa0020610:CDS 35.0%
! CTCCCACATAAACAATTTTG+TGG - chr1_1:35892494-35892513 None:intergenic 35.0%
! ATTTGTTAAAGGTAACGCGT+TGG - chr1_1:35892597-35892616 None:intergenic 35.0%
TGAATGCATCAAAGAACACA+AGG + chr1_1:35892637-35892656 Msa0020610:CDS 35.0%
GAATCGTTGTAGAAACAATG+TGG + chr1_1:35892958-35892977 Msa0020610:CDS 35.0%
! ATATAGGACTCGTTGTCTTT+TGG - chr1_1:35893005-35893024 None:intergenic 35.0%
GGAAGATATTGAAGGAACTT+TGG - chr1_1:35893059-35893078 None:intergenic 35.0%
!! ATTTTTCAGATGTCGCCAAA+CGG - chr1_1:35893081-35893100 None:intergenic 35.0%
TGCTCAAACTCTTGTTCTGA+TGG - chr1_1:35889809-35889828 None:intergenic 40.0%
ATACCAAATTTAGACGCCTC+GGG - chr1_1:35889860-35889879 None:intergenic 40.0%
CATACCAAATTTAGACGCCT+CGG - chr1_1:35889861-35889880 None:intergenic 40.0%
AAACTCGAGTACTTCAGAGA+TGG - chr1_1:35890035-35890054 None:intergenic 40.0%
GACAAAAGAAGCAGGATTAG+AGG - chr1_1:35890306-35890325 None:intergenic 40.0%
TCCGTTAAGACAAAAGAAGC+AGG - chr1_1:35890314-35890333 None:intergenic 40.0%
!! CAGATATCGAAAAAGCGTTC+TGG + chr1_1:35890601-35890620 Msa0020610:CDS 40.0%
TTCTGGCACGAAATAGTACA+TGG + chr1_1:35890618-35890637 Msa0020610:CDS 40.0%
GAGAAAGGAACAGTTGAGTA+TGG + chr1_1:35890642-35890661 Msa0020610:CDS 40.0%
TGAGTATGGAGTCAATGTTG+AGG + chr1_1:35890656-35890675 Msa0020610:CDS 40.0%
AGTGATCCTGATGATAAGCT+TGG + chr1_1:35890693-35890712 Msa0020610:CDS 40.0%
CTTGTTCCAAGCTTATCATC+AGG - chr1_1:35890702-35890721 None:intergenic 40.0%
TTCCTGTCAACTAAACGCAA+TGG - chr1_1:35890928-35890947 None:intergenic 40.0%
AGCATCCCAATGTAAAGCAT+AGG - chr1_1:35891055-35891074 None:intergenic 40.0%
AATTGAGAGGATGCAGAACT+AGG - chr1_1:35891255-35891274 None:intergenic 40.0%
!! ACATAATGTGCCGGTTTACA+AGG + chr1_1:35891395-35891414 Msa0020610:CDS 40.0%
GAACACGCGAGATTTGATTA+TGG - chr1_1:35891735-35891754 None:intergenic 40.0%
!! ATTGGTGATTGGTTTCCACT+CGG + chr1_1:35892022-35892041 Msa0020610:CDS 40.0%
! TGAATAACGTTTGCTAGCTG+CGG - chr1_1:35892050-35892069 None:intergenic 40.0%
TACAACTCCTCAAAAGCTAG+AGG + chr1_1:35892133-35892152 Msa0020610:CDS 40.0%
!!! TTTGTCACCTCTAGCTTTTG+AGG - chr1_1:35892143-35892162 None:intergenic 40.0%
! GATCCAATTTGCCTTTTCCA+TGG + chr1_1:35892367-35892386 Msa0020610:CDS 40.0%
AGAATAACGAGGTCTTACCA+TGG - chr1_1:35892387-35892406 None:intergenic 40.0%
AGAAGAACTGTTAACTCGCT+AGG + chr1_1:35892863-35892882 Msa0020610:CDS 40.0%
TTGGTACTCATCATCACTGA+TGG - chr1_1:35892986-35893005 None:intergenic 40.0%
TCCTTCAATATCTTCCCGTT+TGG + chr1_1:35893063-35893082 Msa0020610:CDS 40.0%
!! TTTTTCAGATGTCGCCAAAC+GGG - chr1_1:35893080-35893099 None:intergenic 40.0%
TACCAAATTTAGACGCCTCG+GGG - chr1_1:35889859-35889878 None:intergenic 45.0%
! GCGTTTTCCAATAGATTGCG+TGG + chr1_1:35890558-35890577 Msa0020610:CDS 45.0%
ACTAAACGCAATGGAGACTG+TGG - chr1_1:35890919-35890938 None:intergenic 45.0%
CTCCATTGCGTTTAGTTGAC+AGG + chr1_1:35890923-35890942 Msa0020610:CDS 45.0%
CAAGACATCAGGAGAGAACA+TGG - chr1_1:35891370-35891389 None:intergenic 45.0%
!! TAATGTGCCGGTTTACAAGG+CGG + chr1_1:35891398-35891417 Msa0020610:CDS 45.0%
! TTTTGCACCGCCTTGTAAAC+CGG - chr1_1:35891408-35891427 None:intergenic 45.0%
CTCTCTCTCTCTACATGTGT+TGG + chr1_1:35891539-35891558 Msa0020610:intron 45.0%
!! TTGGTGATTGGTTTCCACTC+GGG + chr1_1:35892023-35892042 Msa0020610:CDS 45.0%
TAAGACATCGCTATCGCAGT+TGG + chr1_1:35892231-35892250 Msa0020610:CDS 45.0%
TGACATGCAGCCTTTGCAAT+AGG + chr1_1:35892302-35892321 Msa0020610:CDS 45.0%
GACATGCAGCCTTTGCAATA+GGG + chr1_1:35892303-35892322 Msa0020610:CDS 45.0%
ACGAGGTCTTACCATGGAAA+AGG - chr1_1:35892381-35892400 None:intergenic 45.0%
TGCTGCCAAAAGTCTTCAAC+AGG + chr1_1:35892559-35892578 Msa0020610:CDS 45.0%
GCCAAACGGGAAGATATTGA+AGG - chr1_1:35893067-35893086 None:intergenic 45.0%
ACCAAATTTAGACGCCTCGG+GGG - chr1_1:35889858-35889877 None:intergenic 50.0%
AAGGTGACGACAAACTCTCC+TGG - chr1_1:35891432-35891451 None:intergenic 50.0%
TGCAGCAGCAGAGGAACAAT+AGG + chr1_1:35891702-35891721 Msa0020610:intron 50.0%
!! TGGTGATTGGTTTCCACTCG+GGG + chr1_1:35892024-35892043 Msa0020610:CDS 50.0%
TCTGCAGAAAATTGCCCCCG+AGG + chr1_1:35889841-35889860 Msa0020610:CDS 55.0%
GCAGGATTAGAGGCAGCGAT+TGG - chr1_1:35890296-35890315 None:intergenic 55.0%
TACAAGGCGGTGCAAAAGCC+AGG + chr1_1:35891411-35891430 Msa0020610:CDS 55.0%
GTTCCTCTGCTGCTGCATAG+TGG - chr1_1:35891699-35891718 None:intergenic 55.0%
GCAGGAAGTGAAATTCCGCC+AGG - chr1_1:35891861-35891880 None:intergenic 55.0%
! GCCCCCGAGGCGTCTAAATT+TGG + chr1_1:35889854-35889873 Msa0020610:CDS 60.0%
AATGGAGACTGTGGCAGCCG+CGG - chr1_1:35890910-35890929 None:intergenic 60.0%
CCTCTGCTGCTGCATAGTGG+CGG - chr1_1:35891696-35891715 None:intergenic 60.0%
CCGCCACTATGCAGCAGCAG+AGG + chr1_1:35891693-35891712 Msa0020610:intron 65.0%
TTGCTAGCTGCGGCCCCGAG+TGG - chr1_1:35892040-35892059 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 35889770 35893108 35889770 ID=Msa0020610;Name=Msa0020610
chr1_1 mRNA 35889770 35893108 35889770 ID=Msa0020610-mRNA-1;Parent=Msa0020610;Name=Msa0020610-mRNA-1;_AED=0.12;_eAED=0.13;_QI=0|0|0|1|1|1|9|0|564
chr1_1 exon 35889770 35889875 35889770 ID=Msa0020610-mRNA-1:exon:11400;Parent=Msa0020610-mRNA-1
chr1_1 exon 35890273 35890378 35890273 ID=Msa0020610-mRNA-1:exon:11401;Parent=Msa0020610-mRNA-1
chr1_1 exon 35890518 35890737 35890518 ID=Msa0020610-mRNA-1:exon:11402;Parent=Msa0020610-mRNA-1
chr1_1 exon 35890901 35890954 35890901 ID=Msa0020610-mRNA-1:exon:11403;Parent=Msa0020610-mRNA-1
chr1_1 exon 35891040 35891119 35891040 ID=Msa0020610-mRNA-1:exon:11404;Parent=Msa0020610-mRNA-1
chr1_1 exon 35891206 35891483 35891206 ID=Msa0020610-mRNA-1:exon:11405;Parent=Msa0020610-mRNA-1
chr1_1 exon 35891990 35892388 35891990 ID=Msa0020610-mRNA-1:exon:11406;Parent=Msa0020610-mRNA-1
chr1_1 exon 35892483 35892658 35892483 ID=Msa0020610-mRNA-1:exon:11407;Parent=Msa0020610-mRNA-1
chr1_1 exon 35892833 35893108 35892833 ID=Msa0020610-mRNA-1:exon:11408;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35889770 35889875 35889770 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35890273 35890378 35890273 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35890518 35890737 35890518 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35890901 35890954 35890901 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35891040 35891119 35891040 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35891206 35891483 35891206 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35891990 35892388 35891990 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35892483 35892658 35892483 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
chr1_1 CDS 35892833 35893108 35892833 ID=Msa0020610-mRNA-1:cds;Parent=Msa0020610-mRNA-1
Gene Sequence

>Msa0020610

ATGACGAACATTACTCAAGAATGTCCAACGTATTATCCATCAGAACAAGAGTTTGAGCATCCTTTAGTTTATCTGCAGAAAATTGCCCCCGAGGCGTCTAAATTTGGTATATGCAAAATTGTTTCACCAATCGCTGCCTCTAATCCTGCTTCTTTTGTCTTAACGGAAGAGAAAGGGATTTTAAGTTTAACACAATTATACAGCCTCTTAGGAAAATATACATATCATGAATTTGAAGCTCTTGCAAACAAGGCGTTTTCCAATAGATTGCGTGGTAAAGAAGATCTCTCTTGTTCAGATATCGAAAAAGCGTTCTGGCACGAAATAGTACATGGAGAGAAAGGAACAGTTGAGTATGGAGTCAATGTTGAGGAAAGCGCCTTTTCAAGTGATCCTGATGATAAGCTTGGAACAAGCAATTTCAATTTGAAGAATTTGCCGCGGCTGCCACAGTCTCCATTGCGTTTAGTTGACAGGAAAATTCCAGGATTAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTTGAAGATCACTATTTGTACAGCATAAATTACCATCACTCAGGTGCAAATAAAACTTGGTATGGTGTGCCTAGTTCTGCATCCTCTCAATTTGAAAAGACTGTCTTGAATCATGTGTATTGCAACAAAGTCTTAAAAGAACATGGAGAGAATGGTGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTAGAACATAATGTGCCGGTTTACAAGGCGGTGCAAAAGCCAGGAGAGTTTGTCGTCACCTTTCCAAATTCATATCATGCTGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCACTCGGGGCCGCAGCTAGCAAACGTTATTCACATCTCAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCAAAAGCTAGAGGTGACAAAATCAAACTTGAAGATACGGCAACTTATCGAGCTATTGCACTATCTTTCTTGCATCTTATTCAATTTTATAAGACATCGCTATCGCAGTTGGACAGTTCGAAAAAGTTGTCAAGCTCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTTTGCAATAGGGATTGTTATGTAGCTTTCTTGTTGTGCAAGAAATGCTATTCAGATCCAATTTGCCTTTTCCATGACAATGTGCCACAAAATTGTTTATGTGGGAGAGAACATACTGTATTTAAGAGGAATGACATGTTGGAACTCGAAGATGCTGCCAAAAGTCTTCAACAGGAAAAAGAATACACCAACGCGTTACCTTTAACAAATATATTCTCCTATAACATAAATGAATGCATCAAAGAACACAAGAATAATAATTGGCATGAGGAAAAAAGTGCTAGAAGAACTGTTAACTCGCTAGGTACAACATCTAAACTGAAAACAAGAGCTGCGAACAACATCAAGCACACTGTTGAAAATAATCAAAAGACAAAGAATCGTTGTAGAAACAATGTGGCTCCATCAGTGATGATGAGTACCAAAAGACAACGAGTCCTATATAACTTGAGAAAACATGAATCGGAACTGATTACCAAAGTTCCTTCAATATCTTCCCGTTTGGCGACATCTGAAAAATCAAATTAA
Protein sequence

>Msa0020610

MTNITQECPTYYPSEQEFEHPLVYLQKIAPEASKFGICKIVSPIAASNPASFVLTEEKGILSLTQLYSLLGKYTYHEFEALANKAFSNRLRGKEDLSCSDIEKAFWHEIVHGEKGTVEYGVNVEESAFSSDPDDKLGTSNFNLKNLPRLPQSPLRLVDRKIPGLTDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGANKTWYGVPSSASSQFEKTVLNHVYCNKVLKEHGENGAFQFLAQKTTMFSPDVLLEHNVPVYKAVQKPGEFVVTFPNSYHAGFSHGFNCGEAVNFAIGDWFPLGAAASKRYSHLKMLPIIPYEELLCKEALLIYNSSKARGDKIKLEDTATYRAIALSFLHLIQFYKTSLSQLDSSKKLSSSSNTSLGSVTCSLCNRDCYVAFLLCKKCYSDPICLFHDNVPQNCLCGREHTVFKRNDMLELEDAAKSLQQEKEYTNALPLTNIFSYNINECIKEHKNNNWHEEKSARRTVNSLGTTSKLKTRAANNIKHTVENNQKTKNRCRNNVAPSVMMSTKRQRVLYNLRKHESELITKVPSISSRLATSEKSN*