AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0020620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0020620 MtrunA17_Chr1g0164761 87.759 580 51 3 1 579 1 561 0.0 1046
Msa0020620 MtrunA17_Chr1g0164731 76.049 572 109 3 2 571 39 584 0.0 868
Msa0020620 MtrunA17_Chr5g0410721 52.410 498 231 2 2 494 83 579 0.0 552
Msa0020620 MtrunA17_Chr3g0116441 38.056 360 187 8 8 346 18 362 7.06e-67 236
Msa0020620 MtrunA17_Chr8g0380981 31.373 561 277 14 4 477 39 578 4.55e-58 210
Msa0020620 MtrunA17_Chr7g0214951 77.500 120 9 2 181 299 99 201 1.99e-56 189
Msa0020620 MtrunA17_Chr1g0189211 29.981 537 283 16 6 463 148 670 1.01e-54 200
Msa0020620 MtrunA17_Chr1g0199511 45.745 188 98 1 152 339 291 474 3.55e-53 196
Msa0020620 MtrunA17_Chr2g0303101 32.512 406 201 9 4 344 48 445 1.12e-51 191
Msa0020620 MtrunA17_Chr5g0397501 42.264 265 123 7 109 354 323 576 4.71e-51 189
Msa0020620 MtrunA17_Chr7g0261401 30.931 333 183 12 156 462 338 649 3.06e-30 126
Msa0020620 MtrunA17_Chr7g0214941 72.603 73 19 1 470 541 2 74 1.23e-24 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0020620 AT5G46910.2 54.936 466 209 1 7 472 102 566 0.0 543
Msa0020620 AT5G46910.1 54.936 466 209 1 7 472 102 566 0.0 543
Msa0020620 AT2G34880.1 31.144 533 278 15 7 467 60 575 2.29e-59 212
Msa0020620 AT3G48430.1 34.783 368 198 6 9 339 21 383 7.08e-58 210
Msa0020620 AT1G30810.3 29.685 539 291 13 4 467 55 580 5.38e-57 205
Msa0020620 AT1G30810.2 29.685 539 291 13 4 467 55 580 5.38e-57 205
Msa0020620 AT1G30810.1 29.685 539 291 13 4 467 55 580 5.38e-57 205
Msa0020620 AT1G63490.1 36.267 375 170 11 94 431 53 395 5.51e-57 207
Msa0020620 AT1G63490.4 36.267 375 170 11 94 431 306 648 7.39e-57 207
Msa0020620 AT1G63490.2 36.267 375 170 11 94 431 306 648 7.82e-57 207
Msa0020620 AT4G20400.1 27.687 614 327 18 4 529 52 636 1.02e-56 206
Msa0020620 AT4G20400.2 27.907 602 317 18 16 529 7 579 7.74e-54 197
Msa0020620 AT1G08620.3 31.951 410 199 8 6 343 158 559 4.71e-53 196
Msa0020620 AT5G04240.1 46.277 188 97 1 152 339 259 442 4.83e-52 193
Msa0020620 AT1G08620.2 40.000 240 124 4 117 343 313 545 5.56e-45 172
Msa0020620 AT1G08620.4 40.000 240 124 4 117 343 313 545 5.56e-45 172
Msa0020620 AT1G08620.1 40.000 240 124 4 117 343 313 545 5.56e-45 172
Msa0020620 AT2G38950.1 24.723 631 368 20 2 550 102 707 1.02e-34 140
Msa0020620 AT1G63490.3 34.848 198 86 5 254 431 15 189 2.83e-24 108

Find 84 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAATTGTACAGCCTCTT+AGG 0.195974 1_1:+35934080 Msa0020620:CDS
GATGCATTCATTTATGTTAT+AGG 0.215836 1_1:-35936334 None:intergenic
TAGGAGAATATATTTGTTAA+AGG 0.243804 1_1:-35936315 None:intergenic
AATCCAGCATGATATGAATT+TGG 0.254423 1_1:-35935177 None:intergenic
TTCTTTACCACGCAATCTAT+TGG 0.264442 1_1:-35934289 None:intergenic
TGTCTTAACGGAAGAGAAAA+AGG 0.267528 1_1:+35934045 Msa0020620:CDS
TCCTTCAATATCTTCCCTTT+TGG 0.303945 1_1:+35936770 Msa0020620:CDS
ACTGAAAACAGAATAATAAT+TGG 0.313275 1_1:+35936532 Msa0020620:intron
AGTTCCTCATATGGAATTAT+AGG 0.316333 1_1:-35935793 None:intergenic
GAGAAAGGAACAGTTGAGTA+TGG 0.324983 1_1:+35934366 Msa0020620:CDS
ATAAAGGACTCGTTGTCTTT+TGG 0.343992 1_1:-35936709 None:intergenic
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1_1:-35935802 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.359872 1_1:-35936078 None:intergenic
AGCTCTTCAAATACATCATT+AGG 0.370354 1_1:+35935982 Msa0020620:CDS
AACAAGCAATTTCAATTTGA+AGG 0.380018 1_1:+35934440 Msa0020620:CDS
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1_1:+35935719 Msa0020620:CDS
TGACATGCAGCCTTTGCAAT+AGG 0.385933 1_1:+35936010 Msa0020620:CDS
CTCCATTGCGTTTAGTTGAC+AGG 0.390855 1_1:+35934647 Msa0020620:CDS
GACAAAAGAAGCAGGATTAG+AGG 0.392437 1_1:-35934026 None:intergenic
GGAAGATATTGAAGGAACTT+TGG 0.410410 1_1:-35936763 None:intergenic
TGCTGCCAAAAGTCTTCAAC+AGG 0.414775 1_1:+35936269 Msa0020620:CDS
CTGATCCTATGCTTTACATT+GGG 0.424948 1_1:+35934771 Msa0020620:CDS
CACAATTGTACAGCCTCTTA+GGG 0.435355 1_1:+35934081 Msa0020620:CDS
CTTGTTCCAAGCTTATCATC+AGG 0.440827 1_1:-35934423 None:intergenic
GACATGCAGCCTTTGCAATA+GGG 0.448222 1_1:+35936011 Msa0020620:CDS
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1_1:+35935712 Msa0020620:CDS
TTGGTGATTGGTTTCCACTC+GGG 0.456519 1_1:+35935731 Msa0020620:CDS
TTTGATTCGAAAACCCTAAG+AGG 0.457545 1_1:-35934094 None:intergenic
GCTACCTATAATTCCATATG+AGG 0.458631 1_1:+35935789 Msa0020620:CDS
TAACTTGAGAAAACATGAAT+CGG 0.462602 1_1:+35936731 Msa0020620:CDS
TGCTCAAACTCTTGTTCTGA+TGG 0.476367 1_1:-35933530 None:intergenic
TACAAGGCGGTGCAAAAGCC+AGG 0.481676 1_1:+35935135 Msa0020620:CDS
TTAAAAGAACATGGAGAGAA+TGG 0.487266 1_1:+35935039 Msa0020620:CDS
ACATAATGTGCCGGTTTACA+AGG 0.487938 1_1:+35935119 Msa0020620:CDS
ATACCAAATTTAGACGCCTC+GGG 0.488483 1_1:-35933581 None:intergenic
TTGAAGATCACTATTTGTAC+AGG 0.488775 1_1:+35934822 Msa0020620:CDS
ATTTGAAGCTCTTGCAAACA+AGG 0.496352 1_1:+35934260 Msa0020620:CDS
TTTCCAAATTCATATCATGC+TGG 0.496493 1_1:+35935174 Msa0020620:CDS
TGCCACAAAATTGTTTATGC+GGG 0.497644 1_1:+35936199 Msa0020620:CDS
ATCATCAGGATCACTTGAAA+AGG 0.501772 1_1:-35934409 None:intergenic
GGTGCAAATAAAACTTGGTA+TGG 0.503293 1_1:+35934949 Msa0020620:CDS
ATTGGTGATTGGTTTCCACT+CGG 0.503752 1_1:+35935730 Msa0020620:CDS
ACGAGGTCTTACCATGGAAA+AGG 0.511206 1_1:-35936086 None:intergenic
GAAATAGTACATGGAGAGAA+AGG 0.511259 1_1:+35934351 Msa0020620:CDS
CAAAATCAAACTTGAAGATA+CGG 0.514277 1_1:+35935867 Msa0020620:CDS
ACTGATCCTATGCTTTACAT+TGG 0.515442 1_1:+35934770 Msa0020620:CDS
AGTGATCCTGATGATAAGCT+TGG 0.516084 1_1:+35934417 Msa0020620:CDS
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1_1:+35934795 Msa0020620:CDS
TGGTGATTGGTTTCCACTCG+GGG 0.521993 1_1:+35935732 Msa0020620:CDS
TCCGTTAAGACAAAAGAAGC+AGG 0.523048 1_1:-35934034 None:intergenic
TAAGACATCGCTATCGCAGT+TGG 0.526345 1_1:+35935939 Msa0020620:CDS
AATTGAGAGGATGCAGAACT+AGG 0.531709 1_1:-35934976 None:intergenic
ACTCAGGTGCAAATAAAACT+TGG 0.537060 1_1:+35934944 Msa0020620:CDS
TTATGTTCTAACAAGACATC+AGG 0.538881 1_1:-35935102 None:intergenic
CTTGTTAGAACATAATGTGC+CGG 0.547471 1_1:+35935110 Msa0020620:CDS
AATGGAGACTGTGGCAGCCG+CGG 0.554838 1_1:-35934631 None:intergenic
GCAGGATTAGAGGCAGCGAT+TGG 0.560174 1_1:-35934016 None:intergenic
AAGGTGACGACAAACTCTCC+TGG 0.561754 1_1:-35935153 None:intergenic
CAAGACATCAGGAGAGAACA+TGG 0.563327 1_1:-35935091 None:intergenic
CATACCAAATTTAGACGCCT+CGG 0.566207 1_1:-35933582 None:intergenic
TGTTCTGATGGATAATACGT+TGG 0.567602 1_1:-35933518 None:intergenic
GCCAAAAGGGAAGATATTGA+AGG 0.571210 1_1:-35936771 None:intergenic
TTCCTGTCAACTAAACGCAA+TGG 0.577093 1_1:-35934649 None:intergenic
AGAAGAACTGTTAACTCACT+AGG 0.579133 1_1:+35936573 Msa0020620:CDS
CATAACAATCCCTATTGCAA+AGG 0.580709 1_1:-35936020 None:intergenic
GTGCCACAAAATTGTTTATG+CGG 0.580719 1_1:+35936198 Msa0020620:CDS
TTGCTAGCTGCGGCCCCGAG+TGG 0.581206 1_1:-35935745 None:intergenic
TTCTGGCACGAAATAGTACA+TGG 0.584729 1_1:+35934342 Msa0020620:CDS
TATCATGCTGGATTTAGTCA+TGG 0.587724 1_1:+35935186 Msa0020620:CDS
ATTTAAGAGAAATGACATGT+TGG 0.608728 1_1:+35936236 Msa0020620:CDS
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1_1:-35934776 None:intergenic
TACAACTCCTCAAAAGCTAG+AGG 0.624733 1_1:+35935841 Msa0020620:CDS
TGAATAACGTTTGCTAGCTG+CGG 0.626370 1_1:-35935755 None:intergenic
AGCATAAATTACCATCACTC+AGG 0.628951 1_1:+35934928 Msa0020620:intron
ACAGAATAATAATTGGCATG+AGG 0.629929 1_1:+35936539 Msa0020620:intron
TACCAAATTTAGACGCCTCG+GGG 0.634434 1_1:-35933580 None:intergenic
AGCATGATATGAATTTGGAA+AGG 0.636839 1_1:-35935172 None:intergenic
AACAAAGTCTTAAAAGAACA+TGG 0.646276 1_1:+35935030 Msa0020620:CDS
TGAATGCATCAAAGAACACA+AGG 0.658928 1_1:+35936347 Msa0020620:CDS
ATTTGTTAAAGGTAACGCGT+CGG 0.660718 1_1:-35936304 None:intergenic
ACCAAATTTAGACGCCTCGG+GGG 0.671194 1_1:-35933579 None:intergenic
ACTAAACGCAATGGAGACTG+TGG 0.698121 1_1:-35934640 None:intergenic
TAATGTGCCGGTTTACAAGG+CGG 0.701604 1_1:+35935122 Msa0020620:CDS
TGAGTATGGAGTCAATGTTG+AGG 0.703101 1_1:+35934380 Msa0020620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTATTCAAAATTTC+AGG + chr1_1:35934742-35934761 Msa0020620:intron 10.0%
!!! TAATTTTATTCAAAATTTCA+GGG + chr1_1:35934743-35934762 Msa0020620:intron 10.0%
!!! TATTGTTTTCTTTTCATTTT+AGG + chr1_1:35933620-35933639 Msa0020620:intron 15.0%
!!! ATTGTTTTCTTTTCATTTTA+GGG + chr1_1:35933621-35933640 Msa0020620:intron 15.0%
!! ATATTTGATCTTAATATTTC+AGG + chr1_1:35933974-35933993 Msa0020620:intron 15.0%
!!! AACAACTTTTAATTCATATT+AGG - chr1_1:35934703-35934722 None:intergenic 15.0%
!! AGAACGATATAATCTAATAT+AGG + chr1_1:35933883-35933902 Msa0020620:intron 20.0%
!!! TAACTTTTTCTAAGAGAAAA+AGG + chr1_1:35934131-35934150 Msa0020620:CDS 20.0%
!! TGATGAAAAATTAACTAACT+TGG + chr1_1:35934197-35934216 Msa0020620:intron 20.0%
!!! CTTATATTTTACGAATAAGT+AGG + chr1_1:35935296-35935315 Msa0020620:intron 20.0%
!!! AATCTGTTTATTTTTGTACA+AGG + chr1_1:35935468-35935487 Msa0020620:intron 20.0%
!!! TTTTTTTCCATAATAATACC+TGG + chr1_1:35935548-35935567 Msa0020620:intron 20.0%
!!! ACATAAGTATTTAAAATAGC+AGG - chr1_1:35935587-35935606 None:intergenic 20.0%
!!! TAGGAGAATATATTTGTTAA+AGG - chr1_1:35936318-35936337 None:intergenic 20.0%
!!! ACTATTTTAATGAAGTTTAG+AGG - chr1_1:35936416-35936435 None:intergenic 20.0%
!!! ATCTGATTGATTTTAACTTT+TGG + chr1_1:35936502-35936521 Msa0020620:intron 20.0%
!! ACTGAAAACAGAATAATAAT+TGG + chr1_1:35936532-35936551 Msa0020620:intron 20.0%
!! ATTTTCTGCAGATAAACTAA+AGG - chr1_1:35933557-35933576 None:intergenic 25.0%
! AGTAGAATTCTTACTATACT+TGG - chr1_1:35933697-35933716 None:intergenic 25.0%
!! AGATTTTACTAAAATCACAG+TGG + chr1_1:35933844-35933863 Msa0020620:intron 25.0%
! AACAAGCAATTTCAATTTGA+AGG + chr1_1:35934440-35934459 Msa0020620:CDS 25.0%
! AACAAAGTCTTAAAAGAACA+TGG + chr1_1:35935030-35935049 Msa0020620:CDS 25.0%
!!! TTGTTTTTTGTGCAAGAAAT+TGG - chr1_1:35935071-35935090 None:intergenic 25.0%
!! AGGTTGATTGAATAATAGAA+AGG + chr1_1:35935320-35935339 Msa0020620:intron 25.0%
! CTTTCTATTATTCAAGCTTA+GGG - chr1_1:35935375-35935394 None:intergenic 25.0%
!! ATCATTTTCTTGTTGAGTTT+AGG + chr1_1:35935676-35935695 Msa0020620:intron 25.0%
! CAAAATCAAACTTGAAGATA+CGG + chr1_1:35935867-35935886 Msa0020620:CDS 25.0%
! TGTCTGAATATAACCATAAT+AGG - chr1_1:35936176-35936195 None:intergenic 25.0%
! ATTTAAGAGAAATGACATGT+TGG + chr1_1:35936236-35936255 Msa0020620:CDS 25.0%
! GATGCATTCATTTATGTTAT+AGG - chr1_1:35936337-35936356 None:intergenic 25.0%
!! CATGTTTTCTCAAGTTATAA+AGG - chr1_1:35936728-35936747 None:intergenic 25.0%
! TAACTTGAGAAAACATGAAT+CGG + chr1_1:35936731-35936750 Msa0020620:CDS 25.0%
AAAGAAAGTTGAGGGATAAA+AGG - chr1_1:35933731-35933750 None:intergenic 30.0%
! TTTATCCCTCAACTTTCTTT+TGG + chr1_1:35933731-35933750 Msa0020620:intron 30.0%
!!! TTTGATTTTAGAATTTGCCG+CGG + chr1_1:35934614-35934633 Msa0020620:intron 30.0%
TACAAAAATTGCTTCCTTAC+TGG - chr1_1:35934679-35934698 None:intergenic 30.0%
TTGAAGATCACTATTTGTAC+AGG + chr1_1:35934822-35934841 Msa0020620:CDS 30.0%
TTAAAAGAACATGGAGAGAA+TGG + chr1_1:35935039-35935058 Msa0020620:CDS 30.0%
TTATGTTCTAACAAGACATC+AGG - chr1_1:35935105-35935124 None:intergenic 30.0%
AGCATGATATGAATTTGGAA+AGG - chr1_1:35935175-35935194 None:intergenic 30.0%
TTTCCAAATTCATATCATGC+TGG + chr1_1:35935174-35935193 Msa0020620:CDS 30.0%
AATCCAGCATGATATGAATT+TGG - chr1_1:35935180-35935199 None:intergenic 30.0%
! TATTTTACGAATAAGTAGGC+AGG + chr1_1:35935300-35935319 Msa0020620:intron 30.0%
! TGAATAATAGAAAGGTACGT+AGG + chr1_1:35935328-35935347 Msa0020620:intron 30.0%
CCTTTCTATTATTCAAGCTT+AGG - chr1_1:35935376-35935395 None:intergenic 30.0%
CCTAAGCTTGAATAATAGAA+AGG + chr1_1:35935373-35935392 Msa0020620:intron 30.0%
! TTTTCCATAATAATACCTGG+CGG + chr1_1:35935551-35935570 Msa0020620:intron 30.0%
!!! TTGAGTTTAGGTTTTAACTG+TGG + chr1_1:35935688-35935707 Msa0020620:intron 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG + chr1_1:35935719-35935738 Msa0020620:CDS 30.0%
AGTTCCTCATATGGAATTAT+AGG - chr1_1:35935796-35935815 None:intergenic 30.0%
TTACAAAGAAGTTCCTCATA+TGG - chr1_1:35935805-35935824 None:intergenic 30.0%
AGCTCTTCAAATACATCATT+AGG + chr1_1:35935982-35936001 Msa0020620:CDS 30.0%
AATACTCAAAGAGAATAACG+AGG - chr1_1:35936106-35936125 None:intergenic 30.0%
! TAAGTTGCTTGTGTTATTGA+TGG + chr1_1:35936141-35936160 Msa0020620:intron 30.0%
ATGGATAAACTTGCCTATTA+TGG + chr1_1:35936160-35936179 Msa0020620:intron 30.0%
! TTTTTCCTGTTGAAGACTTT+TGG - chr1_1:35936277-35936296 None:intergenic 30.0%
AAACACATTGCTTTATCTTC+AGG + chr1_1:35936463-35936482 Msa0020620:intron 30.0%
ACAGAATAATAATTGGCATG+AGG + chr1_1:35936539-35936558 Msa0020620:intron 30.0%
! TGTTCTGATGGATAATACGT+TGG - chr1_1:35933521-35933540 None:intergenic 35.0%
TGGATCCAAAAGAAAGTTGA+GGG - chr1_1:35933739-35933758 None:intergenic 35.0%
ATGGATCCAAAAGAAAGTTG+AGG - chr1_1:35933740-35933759 None:intergenic 35.0%
! GCAACAGATTCTGCATTTTT+AGG + chr1_1:35933918-35933937 Msa0020620:intron 35.0%
! TCCTGCTTCTTTTGTCTTAA+CGG + chr1_1:35934033-35934052 Msa0020620:CDS 35.0%
TGTCTTAACGGAAGAGAAAA+AGG + chr1_1:35934045-35934064 Msa0020620:CDS 35.0%
! TTTGATTCGAAAACCCTAAG+AGG - chr1_1:35934097-35934116 None:intergenic 35.0%
! GGTTTTCGAATCAAATGAGA+TGG + chr1_1:35934102-35934121 Msa0020620:CDS 35.0%
! ATTTGAAGCTCTTGCAAACA+AGG + chr1_1:35934260-35934279 Msa0020620:CDS 35.0%
TTCTTTACCACGCAATCTAT+TGG - chr1_1:35934292-35934311 None:intergenic 35.0%
GAAATAGTACATGGAGAGAA+AGG + chr1_1:35934351-35934370 Msa0020620:CDS 35.0%
ATCATCAGGATCACTTGAAA+AGG - chr1_1:35934412-35934431 None:intergenic 35.0%
TTGACAGGAAAATTCCAGTA+AGG + chr1_1:35934662-35934681 Msa0020620:intron 35.0%
ACTGATCCTATGCTTTACAT+TGG + chr1_1:35934770-35934789 Msa0020620:CDS 35.0%
CTGATCCTATGCTTTACATT+GGG + chr1_1:35934771-35934790 Msa0020620:CDS 35.0%
TGCTATTCAGTATGTTTGCA+TGG + chr1_1:35934795-35934814 Msa0020620:CDS 35.0%
AGCATAAATTACCATCACTC+AGG + chr1_1:35934928-35934947 Msa0020620:intron 35.0%
!! TTTTATTTGCACCTGAGTGA+TGG - chr1_1:35934942-35934961 None:intergenic 35.0%
ACTCAGGTGCAAATAAAACT+TGG + chr1_1:35934944-35934963 Msa0020620:CDS 35.0%
GGTGCAAATAAAACTTGGTA+TGG + chr1_1:35934949-35934968 Msa0020620:CDS 35.0%
! GACAGTCTTTTCAAATTGAG+AGG - chr1_1:35934992-35935011 None:intergenic 35.0%
CTTGTTAGAACATAATGTGC+CGG + chr1_1:35935110-35935129 Msa0020620:CDS 35.0%
TATCATGCTGGATTTAGTCA+TGG + chr1_1:35935186-35935205 Msa0020620:CDS 35.0%
!! TGGTGTTATTACGTTTCTGA+TGG + chr1_1:35935267-35935286 Msa0020620:intron 35.0%
! AGAAAGGTACGTAGGTTTAA+AGG + chr1_1:35935336-35935355 Msa0020620:intron 35.0%
CGAGATTTGATTATGGAGTT+CGG - chr1_1:35935436-35935455 None:intergenic 35.0%
AATTCCGCCAGGTATTATTA+TGG - chr1_1:35935558-35935577 None:intergenic 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG + chr1_1:35935712-35935731 Msa0020620:CDS 35.0%
GCTACCTATAATTCCATATG+AGG + chr1_1:35935789-35935808 Msa0020620:CDS 35.0%
CATAACAATCCCTATTGCAA+AGG - chr1_1:35936023-35936042 None:intergenic 35.0%
TTACCATGGAAAAGGCAAAT+TGG - chr1_1:35936081-35936100 None:intergenic 35.0%
GTGCCACAAAATTGTTTATG+CGG + chr1_1:35936198-35936217 Msa0020620:CDS 35.0%
TGCCACAAAATTGTTTATGC+GGG + chr1_1:35936199-35936218 Msa0020620:CDS 35.0%
! ATTTGTTAAAGGTAACGCGT+CGG - chr1_1:35936307-35936326 None:intergenic 35.0%
TGAATGCATCAAAGAACACA+AGG + chr1_1:35936347-35936366 Msa0020620:CDS 35.0%
AGAAGAACTGTTAACTCACT+AGG + chr1_1:35936573-35936592 Msa0020620:CDS 35.0%
! ATAAAGGACTCGTTGTCTTT+TGG - chr1_1:35936712-35936731 None:intergenic 35.0%
GGAAGATATTGAAGGAACTT+TGG - chr1_1:35936766-35936785 None:intergenic 35.0%
! TCCTTCAATATCTTCCCTTT+TGG + chr1_1:35936770-35936789 Msa0020620:CDS 35.0%
!! TTTTTCAGATGTCGCCAAAA+GGG - chr1_1:35936787-35936806 None:intergenic 35.0%
!! ATTTTTCAGATGTCGCCAAA+AGG - chr1_1:35936788-35936807 None:intergenic 35.0%
TGCTCAAACTCTTGTTCTGA+TGG - chr1_1:35933533-35933552 None:intergenic 40.0%
ATACCAAATTTAGACGCCTC+GGG - chr1_1:35933584-35933603 None:intergenic 40.0%
CATACCAAATTTAGACGCCT+CGG - chr1_1:35933585-35933604 None:intergenic 40.0%
AAACTCGAGTACTTCAGAGA+TGG - chr1_1:35933759-35933778 None:intergenic 40.0%
GACAAAAGAAGCAGGATTAG+AGG - chr1_1:35934029-35934048 None:intergenic 40.0%
TCCGTTAAGACAAAAGAAGC+AGG - chr1_1:35934037-35934056 None:intergenic 40.0%
ACACAATTGTACAGCCTCTT+AGG + chr1_1:35934080-35934099 Msa0020620:CDS 40.0%
CACAATTGTACAGCCTCTTA+GGG + chr1_1:35934081-35934100 Msa0020620:CDS 40.0%
!! CAGATATCGAAAAAGCGTTC+TGG + chr1_1:35934325-35934344 Msa0020620:CDS 40.0%
TTCTGGCACGAAATAGTACA+TGG + chr1_1:35934342-35934361 Msa0020620:CDS 40.0%
GAGAAAGGAACAGTTGAGTA+TGG + chr1_1:35934366-35934385 Msa0020620:CDS 40.0%
TGAGTATGGAGTCAATGTTG+AGG + chr1_1:35934380-35934399 Msa0020620:CDS 40.0%
AGTGATCCTGATGATAAGCT+TGG + chr1_1:35934417-35934436 Msa0020620:CDS 40.0%
CTTGTTCCAAGCTTATCATC+AGG - chr1_1:35934426-35934445 None:intergenic 40.0%
TTCCTGTCAACTAAACGCAA+TGG - chr1_1:35934652-35934671 None:intergenic 40.0%
AGCATCCCAATGTAAAGCAT+AGG - chr1_1:35934779-35934798 None:intergenic 40.0%
AATTGAGAGGATGCAGAACT+AGG - chr1_1:35934979-35934998 None:intergenic 40.0%
!! ACATAATGTGCCGGTTTACA+AGG + chr1_1:35935119-35935138 Msa0020620:CDS 40.0%
GAACACGCGAGATTTGATTA+TGG - chr1_1:35935443-35935462 None:intergenic 40.0%
!! ATTGGTGATTGGTTTCCACT+CGG + chr1_1:35935730-35935749 Msa0020620:CDS 40.0%
! TGAATAACGTTTGCTAGCTG+CGG - chr1_1:35935758-35935777 None:intergenic 40.0%
TACAACTCCTCAAAAGCTAG+AGG + chr1_1:35935841-35935860 Msa0020620:CDS 40.0%
!!! TTTGTCACCTCTAGCTTTTG+AGG - chr1_1:35935851-35935870 None:intergenic 40.0%
! GATCCAATTTGCCTTTTCCA+TGG + chr1_1:35936075-35936094 Msa0020620:CDS 40.0%
AGAATAACGAGGTCTTACCA+TGG - chr1_1:35936095-35936114 None:intergenic 40.0%
! CTCCCGCATAAACAATTTTG+TGG - chr1_1:35936204-35936223 None:intergenic 40.0%
! CTTTTGGTACTCATCACTGA+TGG - chr1_1:35936696-35936715 None:intergenic 40.0%
GCCAAAAGGGAAGATATTGA+AGG - chr1_1:35936774-35936793 None:intergenic 40.0%
TACCAAATTTAGACGCCTCG+GGG - chr1_1:35933583-35933602 None:intergenic 45.0%
! GCGTTTTCCAATAGATTGCG+TGG + chr1_1:35934282-35934301 Msa0020620:CDS 45.0%
ACTAAACGCAATGGAGACTG+TGG - chr1_1:35934643-35934662 None:intergenic 45.0%
CTCCATTGCGTTTAGTTGAC+AGG + chr1_1:35934647-35934666 Msa0020620:CDS 45.0%
CAAGACATCAGGAGAGAACA+TGG - chr1_1:35935094-35935113 None:intergenic 45.0%
!! TAATGTGCCGGTTTACAAGG+CGG + chr1_1:35935122-35935141 Msa0020620:CDS 45.0%
! TTTTGCACCGCCTTGTAAAC+CGG - chr1_1:35935132-35935151 None:intergenic 45.0%
CTCTCTCTCTCTACATGTGT+TGG + chr1_1:35935247-35935266 Msa0020620:intron 45.0%
!! TTGGTGATTGGTTTCCACTC+GGG + chr1_1:35935731-35935750 Msa0020620:CDS 45.0%
TAAGACATCGCTATCGCAGT+TGG + chr1_1:35935939-35935958 Msa0020620:CDS 45.0%
TGACATGCAGCCTTTGCAAT+AGG + chr1_1:35936010-35936029 Msa0020620:CDS 45.0%
GACATGCAGCCTTTGCAATA+GGG + chr1_1:35936011-35936030 Msa0020620:CDS 45.0%
ACGAGGTCTTACCATGGAAA+AGG - chr1_1:35936089-35936108 None:intergenic 45.0%
TGCTGCCAAAAGTCTTCAAC+AGG + chr1_1:35936269-35936288 Msa0020620:CDS 45.0%
ACCAAATTTAGACGCCTCGG+GGG - chr1_1:35933582-35933601 None:intergenic 50.0%
AAGGTGACGACAAACTCTCC+TGG - chr1_1:35935156-35935175 None:intergenic 50.0%
TGCAGCAGCAGAGGAACAAT+AGG + chr1_1:35935410-35935429 Msa0020620:intron 50.0%
!! TGGTGATTGGTTTCCACTCG+GGG + chr1_1:35935732-35935751 Msa0020620:CDS 50.0%
TCTGCAGAAAATTGCCCCCG+AGG + chr1_1:35933565-35933584 Msa0020620:CDS 55.0%
GCAGGATTAGAGGCAGCGAT+TGG - chr1_1:35934019-35934038 None:intergenic 55.0%
TACAAGGCGGTGCAAAAGCC+AGG + chr1_1:35935135-35935154 Msa0020620:CDS 55.0%
GTTCCTCTGCTGCTGCATAG+TGG - chr1_1:35935407-35935426 None:intergenic 55.0%
GCAGGAAGTGAAATTCCGCC+AGG - chr1_1:35935569-35935588 None:intergenic 55.0%
! GCCCCCGAGGCGTCTAAATT+TGG + chr1_1:35933578-35933597 Msa0020620:CDS 60.0%
AATGGAGACTGTGGCAGCCG+CGG - chr1_1:35934634-35934653 None:intergenic 60.0%
CCTCTGCTGCTGCATAGTGG+CGG - chr1_1:35935404-35935423 None:intergenic 60.0%
CCGCCACTATGCAGCAGCAG+AGG + chr1_1:35935401-35935420 Msa0020620:intron 65.0%
TTGCTAGCTGCGGCCCCGAG+TGG - chr1_1:35935748-35935767 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 35933494 35936815 35933494 ID=Msa0020620;Name=Msa0020620
chr1_1 mRNA 35933494 35936815 35933494 ID=Msa0020620-mRNA-1;Parent=Msa0020620;Name=Msa0020620-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|9|0|580
chr1_1 exon 35933494 35933599 35933494 ID=Msa0020620-mRNA-1:exon:11409;Parent=Msa0020620-mRNA-1
chr1_1 exon 35933996 35934152 35933996 ID=Msa0020620-mRNA-1:exon:11410;Parent=Msa0020620-mRNA-1
chr1_1 exon 35934242 35934461 35934242 ID=Msa0020620-mRNA-1:exon:11411;Parent=Msa0020620-mRNA-1
chr1_1 exon 35934625 35934678 35934625 ID=Msa0020620-mRNA-1:exon:11412;Parent=Msa0020620-mRNA-1
chr1_1 exon 35934764 35934843 35934764 ID=Msa0020620-mRNA-1:exon:11413;Parent=Msa0020620-mRNA-1
chr1_1 exon 35934930 35935207 35934930 ID=Msa0020620-mRNA-1:exon:11414;Parent=Msa0020620-mRNA-1
chr1_1 exon 35935698 35936096 35935698 ID=Msa0020620-mRNA-1:exon:11415;Parent=Msa0020620-mRNA-1
chr1_1 exon 35936193 35936368 35936193 ID=Msa0020620-mRNA-1:exon:11416;Parent=Msa0020620-mRNA-1
chr1_1 exon 35936543 35936815 35936543 ID=Msa0020620-mRNA-1:exon:11417;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35933494 35933599 35933494 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35933996 35934152 35933996 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35934242 35934461 35934242 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35934625 35934678 35934625 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35934764 35934843 35934764 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35934930 35935207 35934930 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35935698 35936096 35935698 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35936193 35936368 35936193 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
chr1_1 CDS 35936543 35936815 35936543 ID=Msa0020620-mRNA-1:cds;Parent=Msa0020620-mRNA-1
Gene Sequence

>Msa0020620

ATGACGAACATTACTCAAGAATGTCCAACGTATTATCCATCAGAACAAGAGTTTGAGCATCCTTTAGTTTATCTGCAGAAAATTGCCCCCGAGGCGTCTAAATTTGGTATATGCAAAATTGTTTCACCAATCGCTGCCTCTAATCCTGCTTCTTTTGTCTTAACGGAAGAGAAAAAGGATTTTAAGTTTAACACAATTGTACAGCCTCTTAGGGTTTTCGAATCAAATGAGATGGATAAAATAACTTTTTCTAAGAGAAAAAGAAAATATACATATCATGAATTTGAAGCTCTTGCAAACAAGGCGTTTTCCAATAGATTGCGTGGTAAAGAAGATCTCTCTTGTTCAGATATCGAAAAAGCGTTCTGGCACGAAATAGTACATGGAGAGAAAGGAACAGTTGAGTATGGAGTCAATGTTGAGGAAAGCGCCTTTTCAAGTGATCCTGATGATAAGCTTGGAACAAGCAATTTCAATTTGAAGAATTTGCCGCGGCTGCCACAGTCTCCATTGCGTTTAGTTGACAGGAAAATTCCAGGATTAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTTGAAGATCACTATTTGTACAGCATAAATTACCATCACTCAGGTGCAAATAAAACTTGGTATGGTGTGCCTAGTTCTGCATCCTCTCAATTTGAAAAGACTGTCTTGAATCATGTGTATTGCAACAAAGTCTTAAAAGAACATGGAGAGAATGGTGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTAGAACATAATGTGCCGGTTTACAAGGCGGTGCAAAAGCCAGGAGAGTTTGTCGTCACCTTTCCAAATTCATATCATGCTGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCACTCGGGGCCGCAGCTAGCAAACGTTATTCACATCTCAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCAAAAGCTAGAGGTGACAAAATCAAACTTGAAGATACGGCAACTTATCGAGCTATTGCACTATCTTTCTTGCATCTTATTCAATTTTATAAGACATCGCTATCGCAGTTGGACAGTTCGAAAAAGTTGTCAAGCTCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTTTGCAATAGGGATTGTTATGTAGCTTTCTTGTTGTGCAAGAAATGCTATTCAGATCCAATTTGCCTTTTCCATGACAATGTGCCACAAAATTGTTTATGCGGGAGAGAACATACTGTATTTAAGAGAAATGACATGTTGGAACTCGAAGCTGCTGCCAAAAGTCTTCAACAGGAAAAAGAATGCACCGACGCGTTACCTTTAACAAATATATTCTCCTATAACATAAATGAATGCATCAAAGAACACAAGAATAATAATTGGCATGAGGAAAAAAGTGCTAGAAGAACTGTTAACTCACTAGGTGCAACATCTAAACTGAAAACAAGAGCTGCGAACAACATCAAGCTCACTGTTGAAAATAATCAAAAGACAAAGAATCGTTGTAGAAACAATGTGTATCCATCAGTGATGAGTACCAAAAGACAACGAGTCCTTTATAACTTGAGAAAACATGAATCGGAACTGATTACCAAAGTTCCTTCAATATCTTCCCTTTTGGCGACATCTGAAAAATCAAATTAA
Protein sequence

>Msa0020620

MTNITQECPTYYPSEQEFEHPLVYLQKIAPEASKFGICKIVSPIAASNPASFVLTEEKKDFKFNTIVQPLRVFESNEMDKITFSKRKRKYTYHEFEALANKAFSNRLRGKEDLSCSDIEKAFWHEIVHGEKGTVEYGVNVEESAFSSDPDDKLGTSNFNLKNLPRLPQSPLRLVDRKIPGLTDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGANKTWYGVPSSASSQFEKTVLNHVYCNKVLKEHGENGAFQFLAQKTTMFSPDVLLEHNVPVYKAVQKPGEFVVTFPNSYHAGFSHGFNCGEAVNFAIGDWFPLGAAASKRYSHLKMLPIIPYEELLCKEALLIYNSSKARGDKIKLEDTATYRAIALSFLHLIQFYKTSLSQLDSSKKLSSSSNTSLGSVTCSLCNRDCYVAFLLCKKCYSDPICLFHDNVPQNCLCGREHTVFKRNDMLELEAAAKSLQQEKECTDALPLTNIFSYNINECIKEHKNNNWHEEKSARRTVNSLGATSKLKTRAANNIKLTVENNQKTKNRCRNNVYPSVMSTKRQRVLYNLRKHESELITKVPSISSLLATSEKSN*