AlfalfaGEDB | Msa0021690

M. sativa cultivar zhongmu-4 / Msa0021690

KEGG: K13412
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004697 GO:0004698 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0021690	sp\|Q6NLQ6\|CDPKW_ARATH	68.984	187	36	2	42	217	218	393	7.23e-82	256

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0021690	MtrunA17_Chr1g0173541	73.262	187	28	2	42	217	207	382	4.66e-90	274
Msa0021690	MtrunA17_Chr7g0257751	74.033	181	37	2	42	217	213	388	4.71e-87	266
Msa0021690	MtrunA17_Chr4g0059951	61.497	187	50	2	42	217	228	403	1.80e-72	229
Msa0021690	MtrunA17_Chr7g0242561	60.000	195	55	3	34	217	204	386	2.10e-70	223
Msa0021690	MtrunA17_Chr5g0448721	56.425	179	72	2	42	217	251	426	2.32e-62	203
Msa0021690	MtrunA17_Chr4g0033371	53.191	188	66	2	42	218	227	403	1.06e-61	201
Msa0021690	MtrunA17_Chr0c02g0489761	54.872	195	64	3	35	218	228	409	6.37e-61	199
Msa0021690	MtrunA17_Chr5g0397101	54.098	183	63	2	45	217	225	396	2.69e-60	197
Msa0021690	MtrunA17_Chr8g0385341	52.261	199	80	3	26	217	170	360	3.71e-60	196
Msa0021690	MtrunA17_Chr1g0200601	49.749	199	99	1	19	217	163	360	3.96e-60	196

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0021690	AT3G57530.2	68.984	187	36	2	42	217	218	393	1.03e-83	255
Msa0021690	AT3G57530.1	68.984	187	36	2	42	217	218	393	7.35e-83	256
Msa0021690	AT5G12480.2	69.101	178	51	2	42	217	120	295	2.01e-80	247
Msa0021690	AT5G12480.1	69.101	178	51	2	42	217	214	389	2.29e-79	247
Msa0021690	AT2G41860.1	71.910	178	46	2	42	217	209	384	3.74e-79	246
Msa0021690	AT2G41860.2	71.348	178	47	2	42	217	209	384	1.03e-78	245
Msa0021690	AT5G19450.2	69.101	178	51	2	42	217	212	387	1.43e-78	245
Msa0021690	AT5G19450.1	69.101	178	51	2	42	217	212	387	1.43e-78	245
Msa0021690	AT3G51850.3	60.513	195	54	3	34	217	202	384	9.84e-72	224
Msa0021690	AT3G51850.2	60.513	195	54	3	34	217	202	384	3.16e-71	225

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Find 64 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCGCCGGCTTACTGCAC+AGG	0.477508	1_1:-39022960	Msa0021690:CDS
AAATCCTCCTAATGTTTCAT+TGG	0.363649	1_1:-39022784	Msa0021690:CDS
AACAGAGGGATTCCAGGTGA+TGG	0.545986	1_1:-39022486	Msa0021690:CDS
AACATTGATGAACTACGAGT+AGG	0.607687	1_1:-39022440	Msa0021690:CDS
AACTAAATGCAGATCATCCA+TGG	0.555527	1_1:-39022822	Msa0021690:intron
AACTTAAGAAAACAGCACTG+AGG	0.760635	1_1:-39022712	Msa0021690:CDS
AATCCTCCTAATGTTTCATT+GGG	0.441297	1_1:-39022783	Msa0021690:CDS
ACACTTCCTGTGCAGTAAGC+CGG	0.574215	1_1:+39022954	None:intergenic
ACATTGATGAACTACGAGTA+GGG	0.566646	1_1:-39022439	Msa0021690:CDS
ACTACGAGTAGGGTTAAGCT+AGG	0.621790	1_1:-39022429	Msa0021690:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAACAATAAACAAAACCGAA+AGG	-	chr1_1:39022209-39022228	Msa0021690:three_prime_UTR	30.0%
GAGAATTCGGGAAAAATTTA+CGG	-	chr1_1:39022349-39022368	Msa0021690:three_prime_UTR	30.0%
CTATGCAAAGATAGAAATAC+TGG	-	chr1_1:39022408-39022427	Msa0021690:three_prime_UTR	30.0%
AGCGTGTAAATCAATTTCAA+AGG	-	chr1_1:39022440-39022459	Msa0021690:CDS	30.0%
TATTGAAGATGTTAGAAGAG+AGG	-	chr1_1:39022485-39022504	Msa0021690:CDS	30.0%
TCTTGGTCGGATATATAATA+TGG	-	chr1_1:39022785-39022804	Msa0021690:CDS	30.0%
ACTAGTAAATAGCATTTACC+TGG	+	chr1_1:39023231-39023250	None:intergenic	30.0%
TTTAGTTAACTGTGTATGGA+TGG	+	chr1_1:39023314-39023333	None:intergenic	30.0%
GAAAGATTTAACGAGATAGT+TGG	-	chr1_1:39023354-39023373	Msa0021690:intron	30.0%
TATCTTGCCAAAGCTATATT+TGG	-	chr1_1:39023505-39023524	Msa0021690:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	39022177	39025012	39022177	ID=Msa0021690;Name=Msa0021690
chr1_1	mRNA	39022177	39025012	39022177	ID=Msa0021690-mRNA-1;Parent=Msa0021690;Name=Msa0021690-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|0.25\|0.2\|1\|0.75\|0.8\|5\|253\|218
chr1_1	exon	39022177	39022549	39022177	ID=Msa0021690-mRNA-1:exon:12038;Parent=Msa0021690-mRNA-1
chr1_1	exon	39022712	39022832	39022712	ID=Msa0021690-mRNA-1:exon:12037;Parent=Msa0021690-mRNA-1
chr1_1	exon	39022956	39023103	39022956	ID=Msa0021690-mRNA-1:exon:12036;Parent=Msa0021690-mRNA-1
chr1_1	exon	39023694	39023837	39023694	ID=Msa0021690-mRNA-1:exon:12035;Parent=Msa0021690-mRNA-1
chr1_1	exon	39024889	39025012	39024889	ID=Msa0021690-mRNA-1:exon:12034;Parent=Msa0021690-mRNA-1
chr1_1	CDS	39024889	39025012	39024889	ID=Msa0021690-mRNA-1:cds;Parent=Msa0021690-mRNA-1
chr1_1	CDS	39023694	39023837	39023694	ID=Msa0021690-mRNA-1:cds;Parent=Msa0021690-mRNA-1
chr1_1	CDS	39022956	39023103	39022956	ID=Msa0021690-mRNA-1:cds;Parent=Msa0021690-mRNA-1
chr1_1	CDS	39022712	39022832	39022712	ID=Msa0021690-mRNA-1:cds;Parent=Msa0021690-mRNA-1
chr1_1	CDS	39022430	39022549	39022430	ID=Msa0021690-mRNA-1:cds;Parent=Msa0021690-mRNA-1
chr1_1	three_prime_UTR	39022177	39022429	39022177	ID=Msa0021690-mRNA-1:three_prime_utr;Parent=Msa0021690-mRNA-1

Gene Sequence

>Msa0021690

ATGGGAAATTGCTGTGCAAATCCATCCAGTAGGAACAATAAACAAAACCGAAAGGAAAAGCCTGGAAAATATGAGCAAGTGAAAATAAACAACAAAATACTAGTGATATTAAAGGAACCCACTGGTGAAAGATTTAACGAGATAGTTGGAAGTCCATATTACATGGCCCCTGAAGTATTAAAAAGAAATTATGGCCCTGAAATTGATATCTGGAGTGCTGGAGTTATTCTTTACATCTTACTTTCTGGTGTCAATCCTTTTTGGGCAGAAACTGAGCAGGGAGTTGCTCAAGCAATTTTAAGATCTTTTATTGACTTTACAAGGGTTCCATGGCCAAAGGTTTCTGATAATGCGAAAGACCTTGTTAAGAAGATGCTTGATCCTGACCCAAAGCGCCGGCTTACTGCACAGGAAGTATCATCCATGGTTGCAGAATGCAAAGAAATCCTCCTAATGTTTCATTGGGAGAAACAGTCCGAGCCAGCAAGGCTCACACAATTTTCTGTAATGAACAAACTTAAGAAAACAGCACTGAGGGTGATTGCAGAGCATTTATCTGTAGAAGAAGTTGTTAGAATAACAGAGGGATTCCAGGTGATGGATACAGAAAATAAAGGAAAAATCAACATTGATGAACTACGAGTAGGGTTAAGCTAG

Protein sequence

>Msa0021690

MGNCCANPSSRNNKQNRKEKPGKYEQVKINNKILVILKEPTGERFNEIVGSPYYMAPEVLKRNYGPEIDIWSAGVILYILLSGVNPFWAETEQGVAQAILRSFIDFTRVPWPKVSDNAKDLVKKMLDPDPKRRLTAQEVSSMVAECKEILLMFHWEKQSEPARLTQFSVMNKLKKTALRVIAEHLSVEEVVRITEGFQVMDTENKGKINIDELRVGLS*