AlfalfaGEDB | Msa0024150

M. sativa cultivar zhongmu-4 / Msa0024150

KEGG: K09420|K09422
GO: GO:0000003 GO:0000278 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006109 GO:0006355 GO:0006357 GO:0006935 GO:0007049 GO:0007275 GO:0008150 GO:0009553 GO:0009605 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009960 GO:0009987 GO:0010154 GO:0010183 GO:0010228 GO:0010262 GO:0010439 GO:0010468 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010675 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0040011 GO:0042221 GO:0042304 GO:0042330 GO:0042592 GO:0042762 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043255 GO:0043455 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0044706 GO:0045595 GO:0045691 GO:0045697 GO:0045723 GO:0045834 GO:0045893 GO:0045923 GO:0045935 GO:0045995 GO:0046889 GO:0046890 GO:0048229 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0050896 GO:0050918 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051704 GO:0055081 GO:0055088 GO:0055089 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0080050 GO:0080090 GO:0097159 GO:0140110 GO:1900376 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1904095 GO:2000014 GO:2000026 GO:2000034 GO:2000112 GO:2000241 GO:2000242 GO:2000692 GO:2001141 GO:2001279 GO:2001280
PFAM: PF13921.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0024150	sp\|Q9S7L2\|MYB98_ARATH	58.647	133	54	1	173	305	209	340	1.16e-46	168

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0024150	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0024150	MtrunA17_Chr7g0228401	49.799	498	146	5	1	397	1	495	3.70e-147	427
Msa0024150	MtrunA17_Chr1g0177901	90.566	212	16	1	191	402	4	211	6.35e-140	397
Msa0024150	MtrunA17_Chr7g0228481	50.682	440	152	10	1	399	1	416	1.38e-128	377
Msa0024150	MtrunA17_Chr7g0228531	50.909	440	151	10	1	399	1	416	4.56e-128	375
Msa0024150	MtrunA17_Chr7g0233161	44.981	269	108	4	4	232	82	350	2.47e-68	220
Msa0024150	MtrunA17_Chr7g0233061	37.831	415	175	12	1	389	1	358	1.24e-65	213
Msa0024150	MtrunA17_Chr4g0046181	44.118	272	112	5	1	232	1	272	1.40e-58	192
Msa0024150	MtrunA17_Chr7g0233151	55.556	171	59	5	231	399	1	156	8.70e-50	166
Msa0024150	MtrunA17_Chr3g0117511	60.606	132	51	1	174	305	176	306	1.10e-46	166
Msa0024150	MtrunA17_Chr4g0058991	56.061	132	57	1	174	305	202	332	1.70e-44	160

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0024150	AT4G18770.1	58.647	133	54	1	173	305	209	340	1.18e-47	168
Msa0024150	AT5G40360.1	46.117	206	86	5	120	306	85	284	2.20e-47	166
Msa0024150	AT3G27785.1	54.676	139	60	1	175	310	183	321	6.69e-46	164
Msa0024150	AT5G11050.1	55.147	136	61	0	154	289	78	213	8.46e-44	158
Msa0024150	AT5G58850.1	44.186	172	83	2	178	336	102	273	5.98e-42	153
Msa0024150	AT2G25230.1	47.368	152	78	2	175	325	20	170	7.48e-38	137
Msa0024150	AT5G40430.1	41.566	166	83	4	155	310	20	181	2.35e-33	125
Msa0024150	AT5G11510.3	53.846	104	47	1	181	283	81	184	5.62e-33	131
Msa0024150	AT5G11510.1	53.846	104	47	1	181	283	81	184	5.62e-33	131
Msa0024150	AT5G11510.4	53.846	104	47	1	181	283	81	184	5.62e-33	131

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Find 98 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAGAGATCCCCGCAC+TGG	0.593457	1_1:+46316376	Msa0024150:CDS
AAAAGAAGGTTCACAAAGAC+AGG	0.463694	1_1:+46316792	Msa0024150:CDS
AAAGAAGGTTCACAAAGACA+GGG	0.706074	1_1:+46316793	Msa0024150:CDS
AAAGAATCCAATATAAGAAA+AGG	0.468148	1_1:+46316827	Msa0024150:CDS
AACATCACTAAGACTAGGGT+TGG	0.545875	1_1:-46318900	None:intergenic
AACCGACGAAATAGTTGGAA+AGG	0.594213	1_1:+46318758	Msa0024150:CDS
AACTAGTGATGGTGGTAGAG+AGG	0.582236	1_1:-46316711	None:intergenic
AAGTTTATGAATGGCAGAAG+CGG	0.586961	1_1:+46317131	Msa0024150:CDS
AAGTTTCACCATATTGAAGG+AGG	0.627242	1_1:-46316670	None:intergenic
AATAGATAAGGAATTCTTTC+TGG	0.120943	1_1:-46316326	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGATGTTCCTTATATTGAT+TGG	-	chr1_1:46316471-46316490	None:intergenic	30.0%
TATAAGGAACATCCTCTTAA+TGG	+	chr1_1:46316476-46316495	Msa0024150:CDS	30.0%
ATAAGGAACATCCTCTTAAT+GGG	+	chr1_1:46316477-46316496	Msa0024150:CDS	30.0%
GTTCATTGAAAATGGTTTGA+TGG	-	chr1_1:46316537-46316556	None:intergenic	30.0%
ATCGATGAAAAATTCTCTCT+GGG	-	chr1_1:46316597-46316616	None:intergenic	30.0%
TACACCAAACTATAACGAAA+TGG	+	chr1_1:46316634-46316653	Msa0024150:CDS	30.0%
TAGGATTCTTGTCAAATTGT+TGG	+	chr1_1:46317073-46317092	Msa0024150:intron	30.0%
TCAAATTGTTGGACTATTCT+GGG	+	chr1_1:46317084-46317103	Msa0024150:CDS	30.0%
ACTATTCTGGGTTTAATAAC+TGG	+	chr1_1:46317096-46317115	Msa0024150:CDS	30.0%
CAGATTGCTAAGTTTATGAA+TGG	+	chr1_1:46317122-46317141	Msa0024150:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	46316305	46319105	46316305	ID=Msa0024150;Name=Msa0024150
chr1_1	mRNA	46316305	46319105	46316305	ID=Msa0024150-mRNA-1;Parent=Msa0024150;Name=Msa0024150-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|3\|0\|402
chr1_1	exon	46316305	46316873	46316305	ID=Msa0024150-mRNA-1:exon:13189;Parent=Msa0024150-mRNA-1
chr1_1	exon	46317076	46317202	46317076	ID=Msa0024150-mRNA-1:exon:13190;Parent=Msa0024150-mRNA-1
chr1_1	exon	46318593	46319105	46318593	ID=Msa0024150-mRNA-1:exon:13191;Parent=Msa0024150-mRNA-1
chr1_1	CDS	46316305	46316873	46316305	ID=Msa0024150-mRNA-1:cds;Parent=Msa0024150-mRNA-1
chr1_1	CDS	46317076	46317202	46317076	ID=Msa0024150-mRNA-1:cds;Parent=Msa0024150-mRNA-1
chr1_1	CDS	46318593	46319105	46318593	ID=Msa0024150-mRNA-1:cds;Parent=Msa0024150-mRNA-1

Gene Sequence

>Msa0024150

ATGGAATTCGATCCAAGTTTTCCAGAAAGAATTCCTTATCTATTTGAAAATTTTCCTAAAATTCCTCTAGAAAAACCAGAGATCCCCGCACTGGTTCTCTTGCAACCAAGTTCTACTCTTACACCTGCACCATCTAATTACACTGGTTTTTTGTACCATAAATCCAATCAATATAAGGAACATCCTCTTAATGGGGTGAACCATCACACTTCTATGGTAGTTCCTTCCTCCATCAAACCATTTTCAATGAACCAAACCCTTTCGCACACGACTGCAAACATCAATGGAACCCAGAGAGAATTTTTCATCGATCAAAATCCAATGACTTTTACACCAAACTATAACGAAATGGAAGCCATGCATGACCTCCTTCAATATGGTGAAACTTCACAATCTCGAGTAATTTCCTCTCTACCACCATCACTAGTTTATGGAAACCAACCAGAAGACCATGATCAAGGAAACATTCTCATTGACCAGATGCGACAAAAGAAGGTTCACAAAGACAGGGAAATTCAACAAAAAGAATCCAATATAAGAAAAGGCCAATGGACTCGTGATGAGGATGGGATTCTTGTCAAATTGTTGGACTATTCTGGGTTTAATAACTGGTCTCAGATTGCTAAGTTTATGAATGGCAGAAGCGGAAAACAGTGCAGGGAGAGATGGCATAACCATCTTCGACCAGACATTAAGAAAGAGTCATGGAGTGAAGAGGAAGATAGAATATTGATCGAAGCTCACAAGATAGTTGGGAACAAGTTTGCTGAAATTGCAAAGAGACTTCCTGGACGTACTGAGAACTCCGTCAAGAACCGTTGGAACTCCGCTAAGCGCAGCCTGAACGCTACAAAGAAGCCGAACCGACGAAATAGTTGGAAAGGAACACTACTTCATAAATACATCAACGAAATCACTAATTCTAAGGATGTTCAGAAGGTGCCGAAAAATTCCACCAACATGATGAACATTGAGTATCAGACAAATTTCGACAATACATATACCAACCCTAGTCTTAGTGATGTTCGATATGAAATTTCAGAGTGTGGTGTTAACTTTGAAGGTTTGGTTACTTCAGTGGAAGAACTTGGTGGCAATGTGACAATGATGTTGAATGGTGATGATGGAATGGCAAGTGGCTCTGGCACAATGAGTCATGAGATTGGATCGTATCAAATGGAGTTTTTTCCTCATGTTAGTGATCTATAG

Protein sequence

>Msa0024150

MEFDPSFPERIPYLFENFPKIPLEKPEIPALVLLQPSSTLTPAPSNYTGFLYHKSNQYKEHPLNGVNHHTSMVVPSSIKPFSMNQTLSHTTANINGTQREFFIDQNPMTFTPNYNEMEAMHDLLQYGETSQSRVISSLPPSLVYGNQPEDHDQGNILIDQMRQKKVHKDREIQQKESNIRKGQWTRDEDGILVKLLDYSGFNNWSQIAKFMNGRSGKQCRERWHNHLRPDIKKESWSEEEDRILIEAHKIVGNKFAEIAKRLPGRTENSVKNRWNSAKRSLNATKKPNRRNSWKGTLLHKYINEITNSKDVQKVPKNSTNMMNIEYQTNFDNTYTNPSLSDVRYEISECGVNFEGLVTSVEELGGNVTMMLNGDDGMASGSGTMSHEIGSYQMEFFPHVSDL*