AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0029940


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0029940 MtrunA17_Chr5g0399781 52.830 53 24 1 4 56 13 64 1.77e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTTGTGGTTTACATTTCA+AGG 0.339580 1_1:+55741643 Msa0029940:CDS
ATTGGTGTCGGCATCTCTAT+TGG 0.346524 1_1:-55742612 None:intergenic
ATTGGTGTCGGCGTCTCTAT+TGG 0.349108 1_1:-55742630 None:intergenic
CCACAAGCGTTACACAATAT+TGG 0.380139 1_1:-55741629 None:intergenic
TTTCAACAGAAACTCAAGTT+TGG 0.389578 1_1:+55741585 Msa0029940:CDS
AATCTTTCAAACCAAACTTC+AGG 0.411413 1_1:+55741758 Msa0029940:CDS
CCAAGCAATGAGATAGGTCT+TGG 0.425187 1_1:+55742759 Msa0029940:CDS
CACAATATTGGCGTCTCGTA+TGG 0.440494 1_1:-55741617 None:intergenic
TCAAACAACAAAATATCGTC+AGG 0.502407 1_1:-55742717 None:intergenic
TCTTGGATGCATTCGTCTAA+AGG 0.502885 1_1:+55742776 Msa0029940:CDS
TTTATACCAAGCAATGAGAT+AGG 0.511374 1_1:+55742753 Msa0029940:CDS
CCAATATTGTGTAACGCTTG+TGG 0.532300 1_1:+55741629 Msa0029940:CDS
GAAACTCAAGTTTGGCGCAA+AGG 0.544030 1_1:+55741593 Msa0029940:CDS
CCAAGACCTATCTCATTGCT+TGG 0.545705 1_1:-55742759 None:intergenic
GGTTTACATTTCAAGGCGAA+AGG 0.567422 1_1:+55741650 Msa0029940:CDS
AATAGAGACGCCGACACCAA+TGG 0.575440 1_1:+55742632 Msa0029940:CDS
GTCGGCGTCTCTATTGGTGT+CGG 0.578211 1_1:-55742624 None:intergenic
TCTTCGGGAGATAGTTAGCA+AGG 0.579240 1_1:-55741676 None:intergenic
GGAAACTCATCCATTGGTGT+CGG 0.599667 1_1:-55742642 None:intergenic
AGCCCTGCAAAATCAGTCAT+CGG 0.602139 1_1:+55741700 Msa0029940:CDS
GATACTTCTACAGATCAAGA+TGG 0.615323 1_1:+55742804 Msa0029940:CDS
CTTCGGGAGATAGTTAGCAA+GGG 0.642252 1_1:-55741675 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAGATGATATAAATTGAA+GGG - chr1_1:55741859-55741878 None:intergenic 15.0%
!! AAATAGATGATATAAATTGA+AGG - chr1_1:55741860-55741879 None:intergenic 15.0%
!!! ACATTGTAAGAATATTTTTA+AGG + chr1_1:55742044-55742063 Msa0029940:intron 15.0%
!!! CATTGTAAGAATATTTTTAA+GGG + chr1_1:55742045-55742064 Msa0029940:intron 15.0%
!!! ATTGTAAGAATATTTTTAAG+GGG + chr1_1:55742046-55742065 Msa0029940:intron 15.0%
!! TTAAAATATGAAACTTCATT+TGG - chr1_1:55742233-55742252 None:intergenic 15.0%
!! AATAGATGATATAAATTGAA+GGG - chr1_1:55741859-55741878 None:intergenic 15.0%
!! AAATAGATGATATAAATTGA+AGG - chr1_1:55741860-55741879 None:intergenic 15.0%
!!! ACATTGTAAGAATATTTTTA+AGG + chr1_1:55742044-55742063 Msa0029940:intron 15.0%
!!! CATTGTAAGAATATTTTTAA+GGG + chr1_1:55742045-55742064 Msa0029940:intron 15.0%
!!! ATTGTAAGAATATTTTTAAG+GGG + chr1_1:55742046-55742065 Msa0029940:intron 15.0%
!! TTAAAATATGAAACTTCATT+TGG - chr1_1:55742233-55742252 None:intergenic 15.0%
!!! AATTTTTATTACCTGAAGTT+TGG - chr1_1:55741772-55741791 None:intergenic 20.0%
!!! AATTTTTATTACCTGAAGTT+TGG - chr1_1:55741772-55741791 None:intergenic 20.0%
!! TTTACATTTGGTGAAATATG+GGG - chr1_1:55741818-55741837 None:intergenic 25.0%
!! CTTTACATTTGGTGAAATAT+GGG - chr1_1:55741819-55741838 None:intergenic 25.0%
!! AATAACTTGTGCTTTACATT+TGG - chr1_1:55741830-55741849 None:intergenic 25.0%
! TCGATTGAAAATGAAAATGT+TGG + chr1_1:55741963-55741982 Msa0029940:intron 25.0%
! CGATTGAAAATGAAAATGTT+GGG + chr1_1:55741964-55741983 Msa0029940:intron 25.0%
!! ATGGGTATTGTTATAATTCA+GGG + chr1_1:55742077-55742096 Msa0029940:intron 25.0%
! ACAAATATCTCCATAAATGT+AGG - chr1_1:55742327-55742346 None:intergenic 25.0%
! GTGGGAAAAATACAAATTTA+TGG + chr1_1:55742523-55742542 Msa0029940:intron 25.0%
!! TTTACATTTGGTGAAATATG+GGG - chr1_1:55741818-55741837 None:intergenic 25.0%
!! CTTTACATTTGGTGAAATAT+GGG - chr1_1:55741819-55741838 None:intergenic 25.0%
!! AATAACTTGTGCTTTACATT+TGG - chr1_1:55741830-55741849 None:intergenic 25.0%
! TCGATTGAAAATGAAAATGT+TGG + chr1_1:55741963-55741982 Msa0029940:intron 25.0%
! CGATTGAAAATGAAAATGTT+GGG + chr1_1:55741964-55741983 Msa0029940:intron 25.0%
!! ATGGGTATTGTTATAATTCA+GGG + chr1_1:55742077-55742096 Msa0029940:intron 25.0%
! ACAAATATCTCCATAAATGT+AGG - chr1_1:55742327-55742346 None:intergenic 25.0%
! GTGGGAAAAATACAAATTTA+TGG + chr1_1:55742523-55742542 Msa0029940:intron 25.0%
TTTCAACAGAAACTCAAGTT+TGG + chr1_1:55741585-55741604 Msa0029940:CDS 30.0%
!!! CAGATGTTTTTTCAATGTCA+AGG - chr1_1:55741727-55741746 None:intergenic 30.0%
AATCTTTCAAACCAAACTTC+AGG + chr1_1:55741758-55741777 Msa0029940:CDS 30.0%
! GCTTTACATTTGGTGAAATA+TGG - chr1_1:55741820-55741839 None:intergenic 30.0%
!! ATTTTTCCTTTTCTAGTGCA+TGG + chr1_1:55741876-55741895 Msa0029940:intron 30.0%
!! TTTTTCCTTTTCTAGTGCAT+GGG + chr1_1:55741877-55741896 Msa0029940:intron 30.0%
ACTCATAAGTAGTGTAGAAT+GGG + chr1_1:55741991-55742010 Msa0029940:intron 30.0%
!! TTTTTAAGGGGTACAATTCA+TGG + chr1_1:55742058-55742077 Msa0029940:intron 30.0%
!! TTTTAAGGGGTACAATTCAT+GGG + chr1_1:55742059-55742078 Msa0029940:intron 30.0%
! CATGGGTATTGTTATAATTC+AGG + chr1_1:55742076-55742095 Msa0029940:intron 30.0%
! TGGGTATTGTTATAATTCAG+GGG + chr1_1:55742078-55742097 Msa0029940:intron 30.0%
!! ATTTTAAGTAGACACAGCAT+TGG + chr1_1:55742246-55742265 Msa0029940:intron 30.0%
ACTCTATATGCCTACATTTA+TGG + chr1_1:55742314-55742333 Msa0029940:intron 30.0%
AATGACAACTAAAGAAGAGT+TGG + chr1_1:55742355-55742374 Msa0029940:intron 30.0%
CTAGCTAATGTGTAACTATA+AGG - chr1_1:55742406-55742425 None:intergenic 30.0%
CACATTAGCTAGTATTCTTA+TGG + chr1_1:55742414-55742433 Msa0029940:intron 30.0%
!!! CATTAGTACCTTATTTTCTC+AGG + chr1_1:55742501-55742520 Msa0029940:intron 30.0%
!!! ACAAATGAACGCTTTTTTTG+AGG - chr1_1:55742561-55742580 None:intergenic 30.0%
! TATAGACTAAGAAGCTTTTG+TGG - chr1_1:55742666-55742685 None:intergenic 30.0%
TCAAACAACAAAATATCGTC+AGG - chr1_1:55742720-55742739 None:intergenic 30.0%
TTTATACCAAGCAATGAGAT+AGG + chr1_1:55742753-55742772 Msa0029940:CDS 30.0%
TTTCAACAGAAACTCAAGTT+TGG + chr1_1:55741585-55741604 Msa0029940:CDS 30.0%
!!! CAGATGTTTTTTCAATGTCA+AGG - chr1_1:55741727-55741746 None:intergenic 30.0%
AATCTTTCAAACCAAACTTC+AGG + chr1_1:55741758-55741777 Msa0029940:CDS 30.0%
! GCTTTACATTTGGTGAAATA+TGG - chr1_1:55741820-55741839 None:intergenic 30.0%
!! ATTTTTCCTTTTCTAGTGCA+TGG + chr1_1:55741876-55741895 Msa0029940:intron 30.0%
!! TTTTTCCTTTTCTAGTGCAT+GGG + chr1_1:55741877-55741896 Msa0029940:intron 30.0%
ACTCATAAGTAGTGTAGAAT+GGG + chr1_1:55741991-55742010 Msa0029940:intron 30.0%
!! TTTTTAAGGGGTACAATTCA+TGG + chr1_1:55742058-55742077 Msa0029940:intron 30.0%
!! TTTTAAGGGGTACAATTCAT+GGG + chr1_1:55742059-55742078 Msa0029940:intron 30.0%
! CATGGGTATTGTTATAATTC+AGG + chr1_1:55742076-55742095 Msa0029940:intron 30.0%
! TGGGTATTGTTATAATTCAG+GGG + chr1_1:55742078-55742097 Msa0029940:intron 30.0%
!! ATTTTAAGTAGACACAGCAT+TGG + chr1_1:55742246-55742265 Msa0029940:intron 30.0%
ACTCTATATGCCTACATTTA+TGG + chr1_1:55742314-55742333 Msa0029940:intron 30.0%
AATGACAACTAAAGAAGAGT+TGG + chr1_1:55742355-55742374 Msa0029940:intron 30.0%
CTAGCTAATGTGTAACTATA+AGG - chr1_1:55742406-55742425 None:intergenic 30.0%
CACATTAGCTAGTATTCTTA+TGG + chr1_1:55742414-55742433 Msa0029940:intron 30.0%
!!! CATTAGTACCTTATTTTCTC+AGG + chr1_1:55742501-55742520 Msa0029940:intron 30.0%
!!! ACAAATGAACGCTTTTTTTG+AGG - chr1_1:55742561-55742580 None:intergenic 30.0%
! TATAGACTAAGAAGCTTTTG+TGG - chr1_1:55742666-55742685 None:intergenic 30.0%
TCAAACAACAAAATATCGTC+AGG - chr1_1:55742720-55742739 None:intergenic 30.0%
TTTATACCAAGCAATGAGAT+AGG + chr1_1:55742753-55742772 Msa0029940:CDS 30.0%
TAAAACCCATGCACTAGAAA+AGG - chr1_1:55741885-55741904 None:intergenic 35.0%
GACTCATAAGTAGTGTAGAA+TGG + chr1_1:55741990-55742009 Msa0029940:intron 35.0%
! TCGTTGCAATGTGGATTAAA+GGG + chr1_1:55742196-55742215 Msa0029940:intron 35.0%
!!! TAGTACCTTATTTTCTCAGG+TGG + chr1_1:55742504-55742523 Msa0029940:intron 35.0%
!!! AGTACCTTATTTTCTCAGGT+GGG + chr1_1:55742505-55742524 Msa0029940:intron 35.0%
! TTTTCCCACCTGAGAAAATA+AGG - chr1_1:55742512-55742531 None:intergenic 35.0%
!! AAAAGCGTTCATTTGTAGAG+AGG + chr1_1:55742565-55742584 Msa0029940:intron 35.0%
! TTTTGTGGAAACTCATCCAT+TGG - chr1_1:55742651-55742670 None:intergenic 35.0%
GATACTTCTACAGATCAAGA+TGG + chr1_1:55742804-55742823 Msa0029940:CDS 35.0%
TAAAACCCATGCACTAGAAA+AGG - chr1_1:55741885-55741904 None:intergenic 35.0%
GACTCATAAGTAGTGTAGAA+TGG + chr1_1:55741990-55742009 Msa0029940:intron 35.0%
! TCGTTGCAATGTGGATTAAA+GGG + chr1_1:55742196-55742215 Msa0029940:intron 35.0%
!!! TAGTACCTTATTTTCTCAGG+TGG + chr1_1:55742504-55742523 Msa0029940:intron 35.0%
!!! AGTACCTTATTTTCTCAGGT+GGG + chr1_1:55742505-55742524 Msa0029940:intron 35.0%
! TTTTCCCACCTGAGAAAATA+AGG - chr1_1:55742512-55742531 None:intergenic 35.0%
!! AAAAGCGTTCATTTGTAGAG+AGG + chr1_1:55742565-55742584 Msa0029940:intron 35.0%
! TTTTGTGGAAACTCATCCAT+TGG - chr1_1:55742651-55742670 None:intergenic 35.0%
GATACTTCTACAGATCAAGA+TGG + chr1_1:55742804-55742823 Msa0029940:CDS 35.0%
CCACAAGCGTTACACAATAT+TGG - chr1_1:55741632-55741651 None:intergenic 40.0%
CCAATATTGTGTAACGCTTG+TGG + chr1_1:55741629-55741648 Msa0029940:CDS 40.0%
CGCTTGTGGTTTACATTTCA+AGG + chr1_1:55741643-55741662 Msa0029940:CDS 40.0%
GGTTTACATTTCAAGGCGAA+AGG + chr1_1:55741650-55741669 Msa0029940:CDS 40.0%
!! GTGCATGGGTTTTAATGTAG+CGG + chr1_1:55741891-55741910 Msa0029940:intron 40.0%
ATAAGTAGTGTAGAATGGGC+AGG + chr1_1:55741995-55742014 Msa0029940:intron 40.0%
! ACGCTGCTAAAGTTTTCTAC+GGG - chr1_1:55742123-55742142 None:intergenic 40.0%
! CACGCTGCTAAAGTTTTCTA+CGG - chr1_1:55742124-55742143 None:intergenic 40.0%
! GTCGTTGCAATGTGGATTAA+AGG + chr1_1:55742195-55742214 Msa0029940:intron 40.0%
!! TGTGGATTAAAGGGTGTTTG+AGG + chr1_1:55742205-55742224 Msa0029940:intron 40.0%
!! GTGGATTAAAGGGTGTTTGA+GGG + chr1_1:55742206-55742225 Msa0029940:intron 40.0%
TCTTGGATGCATTCGTCTAA+AGG + chr1_1:55742776-55742795 Msa0029940:CDS 40.0%
CCACAAGCGTTACACAATAT+TGG - chr1_1:55741632-55741651 None:intergenic 40.0%
CCAATATTGTGTAACGCTTG+TGG + chr1_1:55741629-55741648 Msa0029940:CDS 40.0%
CGCTTGTGGTTTACATTTCA+AGG + chr1_1:55741643-55741662 Msa0029940:CDS 40.0%
GGTTTACATTTCAAGGCGAA+AGG + chr1_1:55741650-55741669 Msa0029940:CDS 40.0%
!! GTGCATGGGTTTTAATGTAG+CGG + chr1_1:55741891-55741910 Msa0029940:intron 40.0%
ATAAGTAGTGTAGAATGGGC+AGG + chr1_1:55741995-55742014 Msa0029940:intron 40.0%
! ACGCTGCTAAAGTTTTCTAC+GGG - chr1_1:55742123-55742142 None:intergenic 40.0%
! CACGCTGCTAAAGTTTTCTA+CGG - chr1_1:55742124-55742143 None:intergenic 40.0%
! GTCGTTGCAATGTGGATTAA+AGG + chr1_1:55742195-55742214 Msa0029940:intron 40.0%
!! TGTGGATTAAAGGGTGTTTG+AGG + chr1_1:55742205-55742224 Msa0029940:intron 40.0%
!! GTGGATTAAAGGGTGTTTGA+GGG + chr1_1:55742206-55742225 Msa0029940:intron 40.0%
TCTTGGATGCATTCGTCTAA+AGG + chr1_1:55742776-55742795 Msa0029940:CDS 40.0%
GAAACTCAAGTTTGGCGCAA+AGG + chr1_1:55741593-55741612 Msa0029940:CDS 45.0%
CACAATATTGGCGTCTCGTA+TGG - chr1_1:55741620-55741639 None:intergenic 45.0%
CTTCGGGAGATAGTTAGCAA+GGG - chr1_1:55741678-55741697 None:intergenic 45.0%
TCTTCGGGAGATAGTTAGCA+AGG - chr1_1:55741679-55741698 None:intergenic 45.0%
! TGATTTTGCAGGGCTCTCTT+CGG - chr1_1:55741695-55741714 None:intergenic 45.0%
AGCCCTGCAAAATCAGTCAT+CGG + chr1_1:55741700-55741719 Msa0029940:CDS 45.0%
!! GTCCGATGACTGATTTTGCA+GGG - chr1_1:55741705-55741724 None:intergenic 45.0%
! CGCTGCTAAAGTTTTCTACG+GGG - chr1_1:55742122-55742141 None:intergenic 45.0%
TGAGAGTTGTCGTTGCAATG+TGG + chr1_1:55742187-55742206 Msa0029940:intron 45.0%
!! ATTGGTGTCGGCATCTCTAT+TGG - chr1_1:55742615-55742634 None:intergenic 45.0%
GGAAACTCATCCATTGGTGT+CGG - chr1_1:55742645-55742664 None:intergenic 45.0%
CCAAGACCTATCTCATTGCT+TGG - chr1_1:55742762-55742781 None:intergenic 45.0%
CCAAGCAATGAGATAGGTCT+TGG + chr1_1:55742759-55742778 Msa0029940:CDS 45.0%
GAAACTCAAGTTTGGCGCAA+AGG + chr1_1:55741593-55741612 Msa0029940:CDS 45.0%
CACAATATTGGCGTCTCGTA+TGG - chr1_1:55741620-55741639 None:intergenic 45.0%
CTTCGGGAGATAGTTAGCAA+GGG - chr1_1:55741678-55741697 None:intergenic 45.0%
TCTTCGGGAGATAGTTAGCA+AGG - chr1_1:55741679-55741698 None:intergenic 45.0%
! TGATTTTGCAGGGCTCTCTT+CGG - chr1_1:55741695-55741714 None:intergenic 45.0%
AGCCCTGCAAAATCAGTCAT+CGG + chr1_1:55741700-55741719 Msa0029940:CDS 45.0%
!! GTCCGATGACTGATTTTGCA+GGG - chr1_1:55741705-55741724 None:intergenic 45.0%
! CGCTGCTAAAGTTTTCTACG+GGG - chr1_1:55742122-55742141 None:intergenic 45.0%
TGAGAGTTGTCGTTGCAATG+TGG + chr1_1:55742187-55742206 Msa0029940:intron 45.0%
!! ATTGGTGTCGGCATCTCTAT+TGG - chr1_1:55742615-55742634 None:intergenic 45.0%
GGAAACTCATCCATTGGTGT+CGG - chr1_1:55742645-55742664 None:intergenic 45.0%
CCAAGACCTATCTCATTGCT+TGG - chr1_1:55742762-55742781 None:intergenic 45.0%
CCAAGCAATGAGATAGGTCT+TGG + chr1_1:55742759-55742778 Msa0029940:CDS 45.0%
! GATTTTGCAGGGCTCTCTTC+GGG - chr1_1:55741694-55741713 None:intergenic 50.0%
!! GGTCCGATGACTGATTTTGC+AGG - chr1_1:55741706-55741725 None:intergenic 50.0%
!! ATTGGTGTCGGCGTCTCTAT+TGG - chr1_1:55742633-55742652 None:intergenic 50.0%
AATAGAGACGCCGACACCAA+TGG + chr1_1:55742632-55742651 Msa0029940:CDS 50.0%
! GATTTTGCAGGGCTCTCTTC+GGG - chr1_1:55741694-55741713 None:intergenic 50.0%
!! GGTCCGATGACTGATTTTGC+AGG - chr1_1:55741706-55741725 None:intergenic 50.0%
!! ATTGGTGTCGGCGTCTCTAT+TGG - chr1_1:55742633-55742652 None:intergenic 50.0%
AATAGAGACGCCGACACCAA+TGG + chr1_1:55742632-55742651 Msa0029940:CDS 50.0%
GTCGGCGTCTCTATTGGTGT+CGG - chr1_1:55742627-55742646 None:intergenic 55.0%
GTCGGCGTCTCTATTGGTGT+CGG - chr1_1:55742627-55742646 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 55741581 55742830 55741581 ID=Msa0029940;Name=Msa0029940
chr1_1 mRNA 55741581 55742830 55741581 ID=Msa0029940-mRNA-1;Parent=Msa0029940;Name=Msa0029940-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|0|0.5|2|0|148
chr1_1 exon 55741581 55741779 55741581 ID=Msa0029940-mRNA-1:exon:16629;Parent=Msa0029940-mRNA-1
chr1_1 exon 55742583 55742830 55742583 ID=Msa0029940-mRNA-1:exon:16630;Parent=Msa0029940-mRNA-1
chr1_1 CDS 55741581 55741779 55741581 ID=Msa0029940-mRNA-1:cds;Parent=Msa0029940-mRNA-1
chr1_1 CDS 55742583 55742830 55742583 ID=Msa0029940-mRNA-1:cds;Parent=Msa0029940-mRNA-1
Gene Sequence

>Msa0029940

ATGGTTTCAACAGAAACTCAAGTTTGGCGCAAAGGACCATACGAGACGCCAATATTGTGTAACGCTTGTGGTTTACATTTCAAGGCGAAAGGAACCCTTGCTAACTATCTCCCGAAGAGAGCCCTGCAAAATCAGTCATCGGACCTTGACATTGAAAAAACATCTGAAAGTAATGCAAATCTTTCAAACCAAACTTCAGAGAGGCATTCATCGACTGTGATGCAGACACCAATAGAGATGCCGACACCAATAGAGACGCCGACACCAATGGATGAGTTTCCACAAAAGCTTCTTAGTCTATATGAATCTGAGAAAGAAGAAAATGAATCATCTCCTGACGATATTTTGTTGTTTGAGAAAATGAACAACTTTATACCAAGCAATGAGATAGGTCTTGGATGCATTCGTCTAAAGGCAGATGATACTTCTACAGATCAAGATGGTTAA

Protein sequence

>Msa0029940

MVSTETQVWRKGPYETPILCNACGLHFKAKGTLANYLPKRALQNQSSDLDIEKTSESNANLSNQTSERHSSTVMQTPIEMPTPIETPTPMDEFPQKLLSLYESEKEENESSPDDILLFEKMNNFIPSNEIGLGCIRLKADDTSTDQDG*