AlfalfaGEDB | Msa0030110

M. sativa cultivar zhongmu-4 / Msa0030110

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF12796.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030110	sp\|Q9LUZ4\|C3H66_ARATH	49.668	602	245	17	21	583	2	584	0.0	530

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0030110	TF	C3H

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030110	MtrunA17_Chr1g0187591	82.236	653	55	3	1	592	1	653	0.0	1029
Msa0030110	MtrunA17_Chr3g0105131	68.551	566	116	7	1	564	1	506	0.0	715
Msa0030110	MtrunA17_Chr5g0430211	53.382	680	215	17	1	591	1	667	0.0	617
Msa0030110	MtrunA17_Chr3g0105101	71.897	427	63	6	37	460	29	401	0.0	582
Msa0030110	MtrunA17_Chr5g0437251	46.341	656	239	15	19	586	13	643	0.0	536
Msa0030110	MtrunA17_Chr7g0257831	40.875	663	254	17	33	586	47	680	2.12e-146	440
Msa0030110	MtrunA17_Chr3g0113651	44.097	576	201	18	31	583	8	485	3.30e-143	424
Msa0030110	MtrunA17_Chr3g0088521	38.529	680	263	23	30	588	42	687	1.59e-128	394
Msa0030110	MtrunA17_Chr3g0105121	52.815	373	141	7	222	582	8	357	7.51e-113	341
Msa0030110	MtrunA17_Chr1g0171151	40.733	464	223	10	33	451	31	487	6.83e-103	323

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030110	AT5G58620.1	49.668	602	245	17	21	583	2	584	0.0	530
Msa0030110	AT3G55980.1	46.000	600	254	14	1	583	1	547	4.18e-167	488
Msa0030110	AT3G55980.2	46.000	600	254	14	1	583	68	614	3.37e-166	488
Msa0030110	AT2G40140.2	46.500	600	259	16	1	583	1	555	4.62e-165	484
Msa0030110	AT2G40140.1	46.500	600	259	16	1	583	1	555	4.62e-165	484
Msa0030110	AT5G12850.1	41.329	617	257	20	25	548	34	638	2.04e-136	414
Msa0030110	AT2G41900.1	47.134	471	182	9	25	447	40	491	8.90e-134	407
Msa0030110	AT4G29190.1	40.702	285	121	10	192	464	49	297	1.51e-57	197
Msa0030110	AT2G19810.1	54.545	154	53	3	208	359	61	199	2.57e-52	183
Msa0030110	AT5G07500.1	60.331	121	48	0	205	325	34	154	4.04e-52	179

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Find 148 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAAGTCATGTGCAA+CGG	0.630659	1_1:+56094251	None:intergenic
AAATTAGACTGGTCCATCCG+CGG	0.660945	1_1:+56095844	Msa0030110:CDS
AACAAAGCAGCAACCATAAG+AGG	0.442942	1_1:-56094497	None:intergenic
AACGTTCTGCCTTCTCTGGT+TGG	0.399281	1_1:+56095806	Msa0030110:CDS
AAGAAATGGGATATGAAGAG+AGG	0.624986	1_1:+56094471	Msa0030110:CDS
AAGCAAAAGTTTGACAACCT+CGG	0.631058	1_1:-56094642	None:intergenic
AAGGAGATGCAAGACCTGTA+AGG	0.541933	1_1:-56095528	None:intergenic
AAGGATTGCCCCAAAGTTGC+TGG	0.360358	1_1:-56095642	None:intergenic
AAGGTTGGATCAAAAGAAAT+GGG	0.451915	1_1:+56094458	Msa0030110:CDS
AAGTAGGGTTATGGTATGGA+AGG	0.522426	1_1:+56094435	Msa0030110:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAGAAAACCTTGTCAAAC+TGG	+	chr1_1:56094278-56094297	Msa0030110:CDS	30.0%
TTGATTAGGGAAAATGAATG+TGG	+	chr1_1:56094302-56094321	Msa0030110:CDS	30.0%
TTCAGCCAAAGATGATATAA+TGG	+	chr1_1:56094370-56094389	Msa0030110:CDS	30.0%
AAGGTTGGATCAAAAGAAAT+GGG	+	chr1_1:56094458-56094477	Msa0030110:CDS	30.0%
TTGTCTTATATTCTTGGAAC+CGG	+	chr1_1:56094536-56094555	Msa0030110:CDS	30.0%
GACGATTTCCTTAAGAAAAT+AGG	+	chr1_1:56094794-56094813	Msa0030110:CDS	30.0%
TTTGAATATGATGTAGGTGA+AGG	-	chr1_1:56095271-56095290	None:intergenic	30.0%
CGTGAATTTGAATATGATGT+AGG	-	chr1_1:56095277-56095296	None:intergenic	30.0%
CTTACCAATTCAAATGTGAT+TGG	+	chr1_1:56095670-56095689	Msa0030110:CDS	30.0%
TGATCCAATCACATTTGAAT+TGG	-	chr1_1:56095677-56095696	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	56094263	56096041	56094263	ID=Msa0030110;Name=Msa0030110
chr1_1	mRNA	56094263	56096041	56094263	ID=Msa0030110-mRNA-1;Parent=Msa0030110;Name=Msa0030110-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|592
chr1_1	exon	56094263	56096041	56094263	ID=Msa0030110-mRNA-1:exon:16752;Parent=Msa0030110-mRNA-1
chr1_1	CDS	56094263	56096041	56094263	ID=Msa0030110-mRNA-1:cds;Parent=Msa0030110-mRNA-1

Gene Sequence

>Msa0030110

ATGTGCAACGGTTCGAATAGAAAACCTTGTCAAACTGGTTTGATTAGGGAAAATGAATGTGGAAGACAAGAAGAACTTCATCATAAGATTTCACCTTTGCTTGAGTTTTCAGCCAAAGATGATATAATGGCGTTCACAAATGCTGTTGAAAAAGATGATCATGATGTTGATGAAGTAGGGTTATGGTATGGAAGGAAGGTTGGATCAAAAGAAATGGGATATGAAGAGAGGACACCTCTTATGGTTGCTGCTTTGTTTGGAAGCAAGGGTGTGTTGTCTTATATTCTTGGAACCGGCCGCGTTGATGTTAACCGAGCGTGCGGCTCAGATAGGGCTACCACTCTTCATTGTGCTGTTTCTGGTTTTTCTGCTGCTTCTGCCGAGGTTGTCAAACTTTTGCTTGATGCATCTGCAGATGTTAGTTCCGCTGATGCAAACGGTAACCGGTGCAGTGACTTGATTGTCTCGGTTTCTAAGAGTATCTTCGGTTCAAGGAAGAGGATACTACAAGGCAATGTTGATGACGAGGACGACGATTTCCTTAAGAAAATAGGTTTCCAAATGGTGGAGCAACAACAAGATGTAGGTACATCTCGAACTGAGAAGAAAGATTATCCCATGGATCCATCCCTACCTGACATAAAGAATGGAATATATAGTACAGATGAATTTAGGATGTTTACATTCAAAGTGAAGCCTTGTTCAAGGGCTTATTCTCATGATTGGACTGAGTGCCCCTTTGTTCAACCTGGGGAGAATGCAAGGCGCCGTGATCCGAGGAAATATCATTACACGTGTGTCCCTTGCCCCGAGTTTCGGAAGGGATCATGCAACAAAGGGGATGCTTGTGAATATGCACATGGTATTTTTGAATGCTGGCTTCATCCTGCTCAATACAGAACAAGGCTTTGCAAGGATGAAACTAGATGCACGAGAAGGGTTTGCTTTTTCGCTCACAAGCCAGAGGAGCTTCGACCGTTGCATGCTTCCACTGGTTCTGCTCTACCTTCACCTACATCATATTCAAATTCACGAAGTGCTTCTTCGATGGATTCTTTCACATTGAGCTCTCCGTCTTCTTCGATACAATCTGCGTCGACGCCACCTTTGACTCCATCTGCAGCATCATCGCCTAAGGCTGGAACCATGTGGCAGACTCAGATTCAGCTTAATGCTGCAGTCCCTACCCTTCAGATGCCGAGAAGCAGATTTAAAACTGCAATGAATGCTAGAAATAATGCTGAATTTCTCGAACTTGAAAATCGCCTTACAGGTCTTGCATCTCCTTCAAATAGGCTTGCAGGAGTGAATCCTACTAGCCTTGAAAACATTTTCGGGTCATCAATACAATCTCCGACATCAATGCATCAGAGCACAAACCAGCAACTTTGGGGCAATCCTTTTGATCTTACCAATTCAAATGTGATTGGATCAACACAATTTAGAGTTGATGCCTTTTCGAAGCGGAGCCAAAGCTTTATTGAGCGCAGCAGCGGTGCGAGCTTTAATCCTGAGCTTCCTTCTGCTAGCTCAGTTGCCATGGAACGTTCTGCCTTCTCTGGTTGGGGCTCTCCTGATGGAAAATTAGACTGGTCCATCCGCGGCGATGAACTCAGTAAAATGAGGAAAACTTATTCTTTTGGCTTTCGAAACAGAAGTAGCACTTCAACAATGGCTGCTGCATTAAGTGTTGGTGATCCAGATATATTTTTAAGCCATGAATCTTGGGTTGATTCACTTGTGAATGATGGTCCAACAATGGAATTTGATCAACATTGA

Protein sequence

>Msa0030110

MCNGSNRKPCQTGLIRENECGRQEELHHKISPLLEFSAKDDIMAFTNAVEKDDHDVDEVGLWYGRKVGSKEMGYEERTPLMVAALFGSKGVLSYILGTGRVDVNRACGSDRATTLHCAVSGFSAASAEVVKLLLDASADVSSADANGNRCSDLIVSVSKSIFGSRKRILQGNVDDEDDDFLKKIGFQMVEQQQDVGTSRTEKKDYPMDPSLPDIKNGIYSTDEFRMFTFKVKPCSRAYSHDWTECPFVQPGENARRRDPRKYHYTCVPCPEFRKGSCNKGDACEYAHGIFECWLHPAQYRTRLCKDETRCTRRVCFFAHKPEELRPLHASTGSALPSPTSYSNSRSASSMDSFTLSSPSSSIQSASTPPLTPSAASSPKAGTMWQTQIQLNAAVPTLQMPRSRFKTAMNARNNAEFLELENRLTGLASPSNRLAGVNPTSLENIFGSSIQSPTSMHQSTNQQLWGNPFDLTNSNVIGSTQFRVDAFSKRSQSFIERSSGASFNPELPSASSVAMERSAFSGWGSPDGKLDWSIRGDELSKMRKTYSFGFRNRSSTSTMAAALSVGDPDIFLSHESWVDSLVNDGPTMEFDQH*