AlfalfaGEDB | Msa0030460

M. sativa cultivar zhongmu-4 / Msa0030460

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030460	sp\|Q9ZVC9\|FRS3_ARATH	36.196	163	95	5	242	398	476	635	6.14e-18	91.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0030460	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030460	MtrunA17_Chr3g0082121	73.913	207	51	2	236	439	486	692	2.69e-90	292
Msa0030460	MtrunA17_Chr3g0082121	64.626	147	51	1	87	233	2	147	8.81e-54	194
Msa0030460	MtrunA17_Chr5g0401121	65.891	129	40	1	105	233	36	160	2.18e-48	165
Msa0030460	MtrunA17_Chr1g0203721	42.012	169	90	3	239	399	518	686	8.24e-28	118
Msa0030460	MtrunA17_Chr1g0203721	37.984	129	78	2	120	247	54	181	1.17e-21	99.4
Msa0030460	MtrunA17_Chr6g0458391	41.447	152	75	4	93	240	194	335	2.10e-26	114
Msa0030460	MtrunA17_Chr6g0458391	47.170	106	52	2	240	341	661	766	1.61e-21	99.0
Msa0030460	MtrunA17_Chr6g0458391	42.857	112	62	2	129	240	91	200	8.96e-19	90.1
Msa0030460	MtrunA17_Chr2g0324011	30.147	272	162	8	104	355	320	583	2.95e-24	107
Msa0030460	MtrunA17_Chr2g0324011	42.478	113	61	3	129	240	10	119	8.41e-16	80.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030460	AT2G27110.2	36.196	163	95	5	242	398	476	635	6.24e-19	91.3
Msa0030460	AT2G27110.1	36.196	163	95	5	242	398	476	635	6.24e-19	91.3
Msa0030460	AT2G27110.3	36.196	163	95	5	242	398	331	490	7.29e-19	90.9
Msa0030460	AT3G22170.2	36.184	152	75	5	244	385	518	657	8.85e-19	90.9
Msa0030460	AT3G22170.1	36.184	152	75	5	244	385	518	657	8.85e-19	90.9
Msa0030460	AT3G59470.3	36.486	148	82	3	96	233	27	172	3.97e-16	79.0
Msa0030460	AT3G59470.1	36.486	148	82	3	96	233	27	172	3.97e-16	79.0
Msa0030460	AT3G59470.2	36.486	148	82	3	96	233	39	184	5.10e-16	79.0
Msa0030460	AT4G12850.4	38.739	111	64	3	122	232	4	110	4.65e-15	72.8
Msa0030460	AT5G18960.2	29.213	178	116	5	227	397	608	782	4.84e-15	79.0

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Find 136 sgRNAs with CRISPR-Local

Find 323 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTATATACTTAAGCGA+TGG	0.590891	1_1:+56827077	Msa0030460:CDS
AAATGATGAAATTGACAATG+TGG	0.705740	1_1:+56827363	Msa0030460:CDS
AAATTTAGTACAGTCGTTAC+CGG	0.437312	1_1:+56827516	Msa0030460:CDS
AACAACAATGGCAGCGGCGC+AGG	0.452252	1_1:-56825816	None:intergenic
AACAGGATGTTTGGCGCGTA+TGG	0.429347	1_1:+56826587	Msa0030460:CDS
AAGATTCACATGCTACAAAG+AGG	0.619398	1_1:+56826506	Msa0030460:CDS
AAGCCACCTCTCCTTCCCAT+CGG	0.662631	1_1:+56825670	Msa0030460:CDS
AAGGAGAGTTTGCAAGCGCG+TGG	0.605662	1_1:+56829682	Msa0030460:CDS
AAGTATGTGGATGGAGAATC+AGG	0.314708	1_1:+56827310	Msa0030460:CDS
AATATTTGAGGAGTTCCAAG+AGG	0.693235	1_1:+56826844	Msa0030460:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATTCTTTGGTTATTCAGG+AGG	+	chr1_1:56824728-56824747	Msa0030460:CDS	30.0%
CTAACCTTTCAAAACAGTAA+AGG	-	chr1_1:56824971-56824990	None:intergenic	30.0%
TTACTTCTTTACTACGTAGT+TGG	+	chr1_1:56825354-56825373	Msa0030460:intron	30.0%
GTAAATTGAGCAAAGTGAAA+TGG	-	chr1_1:56825475-56825494	None:intergenic	30.0%
AATCAATGGCTGTGATTAAA+TGG	+	chr1_1:56825536-56825555	Msa0030460:intron	30.0%
ATTACCTTGATCAACAACAA+TGG	-	chr1_1:56825831-56825850	None:intergenic	30.0%
AGAAATTAGAACAGGATGTT+TGG	+	chr1_1:56826578-56826597	Msa0030460:CDS	30.0%
TTACACTCCTAGAATATTTG+AGG	+	chr1_1:56826832-56826851	Msa0030460:CDS	30.0%
GATATTCTTCATATTCCTCT+TGG	-	chr1_1:56826862-56826881	None:intergenic	30.0%
TGCGTAAAAGATTTGAAAGA+AGG	+	chr1_1:56826890-56826909	Msa0030460:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	56824699	56829732	56824699	ID=Msa0030460;Name=Msa0030460
chr1_1	mRNA	56824699	56829732	56824699	ID=Msa0030460-mRNA-1;Parent=Msa0030460;Name=Msa0030460-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0\|0\|0\|0.66\|1\|1\|6\|0\|557
chr1_1	exon	56824699	56824796	56824699	ID=Msa0030460-mRNA-1:exon:17036;Parent=Msa0030460-mRNA-1
chr1_1	exon	56825637	56825845	56825637	ID=Msa0030460-mRNA-1:exon:17037;Parent=Msa0030460-mRNA-1
chr1_1	exon	56826295	56826692	56826295	ID=Msa0030460-mRNA-1:exon:17038;Parent=Msa0030460-mRNA-1
chr1_1	exon	56826800	56827537	56826800	ID=Msa0030460-mRNA-1:exon:17039;Parent=Msa0030460-mRNA-1
chr1_1	exon	56827629	56827702	56827629	ID=Msa0030460-mRNA-1:exon:17040;Parent=Msa0030460-mRNA-1
chr1_1	exon	56829576	56829732	56829576	ID=Msa0030460-mRNA-1:exon:17041;Parent=Msa0030460-mRNA-1
chr1_1	CDS	56824699	56824796	56824699	ID=Msa0030460-mRNA-1:cds;Parent=Msa0030460-mRNA-1
chr1_1	CDS	56825637	56825845	56825637	ID=Msa0030460-mRNA-1:cds;Parent=Msa0030460-mRNA-1
chr1_1	CDS	56826295	56826692	56826295	ID=Msa0030460-mRNA-1:cds;Parent=Msa0030460-mRNA-1
chr1_1	CDS	56826800	56827537	56826800	ID=Msa0030460-mRNA-1:cds;Parent=Msa0030460-mRNA-1
chr1_1	CDS	56827629	56827702	56827629	ID=Msa0030460-mRNA-1:cds;Parent=Msa0030460-mRNA-1
chr1_1	CDS	56829576	56829732	56829576	ID=Msa0030460-mRNA-1:cds;Parent=Msa0030460-mRNA-1

Gene Sequence

>Msa0030460

ATGGATCCATTTCGTTCCATGAACAAAGAATATTCTTTGGTTATTCAGGAGGAGTGTAATAATAATAGTTCTATTCATGTTCCTGTTGTTGCACCTTTAAAACTTTCCACCTTCCTCCCCCATAATCATCGAAGCCACCTCTCCTTCCCATCGGCTCCGCCGTCACCTCTCCTTCTCTCTCGCCGCTGCACCTCTCTCTTTGCCTCCGTCAACGTCATGAGCGAACCCCATCATCCTGCTACCAACACTAACGAACCCGCCGCCGTCTCCGACCATCCCTGCGCCGCTGCCATTGTTGTTGATCAAGATTGTGTCAATAAATTGGTTAACAATATTCATGAACAAAATGTGCAACACGGACAAGGAAGTATTGCCAACAATGTACCTCACTTGGAAATGAAGTTTGATTCTGAGGCAGCGGCTTATGACTTTTATAATGAGTATAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAGTTGATGGAGTTTTGACTTCAAGAAGATTCACATGCTACAAAGAGGGTGTACGAGGTGTTGACAAAAGACGTCAACCAAAAGGGGAGCCTAGAGCAGAAATTAGAACAGGATGTTTGGCGCGTATGGTTATTTCACTCGATCGGAAGATAGAGAAATATAAAGTTGTTGACTTTATAGCTGAACATAACCACCAACTTCAACCTGAGCATAAGGCTCGTATGCTCATACAAGCTGCAAATGTTTACACTCCTAGAATATTTGAGGAGTTCCAAGAGGAATATGAAGAATATCAAGACACTTGCGTAAAAGATTTGAAAGAAGGTTTGTATACTGTCACAAACTATGATAATCCAACGGAGAGGATAGTTATGGGAAATGCTATGGAACAAAAAGTTTCATGTGATTGTCGTAAATTTGAGACACATGGCATCTTATGCAGTCATGCTTTGAAAGTTTTAGATGTCATGAACATTAAGTTAATTCCTGAAACCTATATACTTAAGCGATGGACAAGAAATGCAAGGTTGGGAAGTGATCAAGATTGGAAAGGGAGGCATGTTGAATTAGACATAAAAGCCCATTTCATGAAGCGGTACAATGAGTTATGTCCTCGAATGGTTAAGTTAACCAACAAAGCTTCAGAAACTCATGAAACTTACACATTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAATTGATGATATGTTGGCCAAAAAGTATGTGGATGGAGAATCAGGGGGCATGGTTCATGTATCCATATCAATTGCAAATGATGAAATTGACAATGTGGACACCGTTGGTATGGCAAAAGGCATAGCTAATCGAGAAAGTTCACGCAAGCGGAAAAAGCGATATAAATCTTGGATTGAGAAAAAAGATAAAAAGACAAAGATGTGCTCCAAGAGAAAGAGATCTGGTGAAAATTTAGTACAGTCGTTACCGCAAGAAACTACAGTTCAAGCGACACAATCAAGTCAAGTTGGCAATGGAACTCAACATCAAGCTACAATCCAAGAAAGGGGGGAGAGTGCGCCAGTGGATGGAGGGAGAGGTGCGGTGGCGGCGGCGGCAGTGGCGGCGGTGGCGATGGGAGGGAGAGAGAGGTGCTATCTGAGAAGGAAGAAGGAGAGTTTGCAAGCGCGTGGAATTTGTTTCAGAGTAGAGAGCGCGTAG

Protein sequence

>Msa0030460

MDPFRSMNKEYSLVIQEECNNNSSIHVPVVAPLKLSTFLPHNHRSHLSFPSAPPSPLLLSRRCTSLFASVNVMSEPHHPATNTNEPAAVSDHPCAAAIVVDQDCVNKLVNNIHEQNVQHGQGSIANNVPHLEMKFDSEAAAYDFYNEYSKRIGFGIRREYGNKSRVDGVLTSRRFTCYKEGVRGVDKRRQPKGEPRAEIRTGCLARMVISLDRKIEKYKVVDFIAEHNHQLQPEHKARMLIQAANVYTPRIFEEFQEEYEEYQDTCVKDLKEGLYTVTNYDNPTERIVMGNAMEQKVSCDCRKFETHGILCSHALKVLDVMNIKLIPETYILKRWTRNARLGSDQDWKGRHVELDIKAHFMKRYNELCPRMVKLTNKASETHETYTFLSKVYEESSKIIDDMLAKKYVDGESGGMVHVSISIANDEIDNVDTVGMAKGIANRESSRKRKKRYKSWIEKKDKKTKMCSKRKRSGENLVQSLPQETTVQATQSSQVGNGTQHQATIQERGESAPVDGGRGAVAAAAVAAVAMGGRERCYLRRKKESLQARGICFRVESA*