AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0031370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0031370 MtrunA17_Chr1g0189391 80.441 363 27 4 142 489 1 334 0.0 548
Msa0031370 MtrunA17_Chr6g0485431 47.445 411 164 8 81 489 1 361 2.04e-112 336
Msa0031370 MtrunA17_Chr7g0249311 57.746 71 29 1 225 295 379 448 2.28e-21 97.4
Msa0031370 MtrunA17_Chr7g0249311 37.778 90 47 3 221 303 155 242 4.46e-11 65.5
Msa0031370 MtrunA17_Chr7g0218081 50.617 81 39 1 215 295 387 466 3.31e-21 96.7
Msa0031370 MtrunA17_Chr7g0218081 43.023 86 43 3 238 323 228 307 1.01e-11 67.0
Msa0031370 MtrunA17_Chr7g0243891 34.574 188 104 7 158 331 280 462 3.49e-21 96.7
Msa0031370 MtrunA17_Chr8g0383251 50.667 75 36 1 221 295 91 164 8.92e-21 89.7
Msa0031370 MtrunA17_Chr4g0042831 46.875 96 49 2 201 295 240 334 9.67e-21 94.0
Msa0031370 MtrunA17_Chr3g0079691 40.580 138 72 6 177 313 439 567 3.33e-20 94.0
Msa0031370 MtrunA17_Chr5g0418191 47.826 92 47 1 225 316 307 397 4.12e-20 92.8
Msa0031370 MtrunA17_Chr7g0249301 54.930 71 31 1 225 295 279 348 5.79e-20 91.7
Msa0031370 MtrunA17_Chr1g0150801 50.667 75 36 1 221 295 85 158 6.27e-20 86.7
Msa0031370 MtrunA17_Chr5g0445311 37.226 137 82 2 159 295 288 420 7.76e-20 92.4
Msa0031370 MtrunA17_Chr3g0129361 52.055 73 34 1 225 297 131 202 9.62e-20 87.8
Msa0031370 MtrunA17_Chr4g0004911 44.762 105 56 2 191 295 290 392 1.07e-19 91.7
Msa0031370 MtrunA17_Chr8g0383351 53.521 71 32 1 230 300 152 221 1.66e-19 87.4
Msa0031370 MtrunA17_Chr3g0101131 53.521 71 32 1 225 295 402 471 3.09e-19 90.9
Msa0031370 MtrunA17_Chr3g0106491 54.930 71 31 1 225 295 352 421 3.31e-19 90.5
Msa0031370 MtrunA17_Chr3g0106491 43.333 90 42 4 238 326 198 279 3.33e-11 65.5
Msa0031370 MtrunA17_Chr5g0432161 54.930 71 31 1 225 295 408 477 4.57e-19 90.1
Msa0031370 MtrunA17_Chr7g0270191 41.748 103 47 3 225 326 132 222 7.81e-19 87.0
Msa0031370 MtrunA17_Chr4g0051881 43.810 105 51 4 225 329 165 261 5.44e-18 85.1
Msa0031370 MtrunA17_Chr3g0129321 48.000 75 38 1 221 295 101 174 6.22e-18 82.4
Msa0031370 MtrunA17_Chr8g0335811 48.684 76 38 1 225 300 141 215 1.07e-17 82.0
Msa0031370 MtrunA17_Chr4g0068541 48.684 76 37 2 225 299 195 269 3.30e-17 83.6
Msa0031370 MtrunA17_Chr7g0263531 51.429 70 33 1 226 295 349 417 4.06e-17 83.6
Msa0031370 MtrunA17_Chr1g0194481 49.296 71 35 1 225 295 145 214 6.47e-17 81.6
Msa0031370 MtrunA17_Chr5g0447701 45.205 73 39 1 225 297 239 310 2.03e-16 80.9
Msa0031370 MtrunA17_Chr7g0224801 52.857 70 28 2 228 295 83 149 2.52e-16 76.6
Msa0031370 MtrunA17_Chr1g0188941 50.725 69 33 1 227 295 40 107 3.52e-16 74.7
Msa0031370 MtrunA17_Chr2g0294231 49.296 71 35 1 225 295 190 259 3.90e-16 80.1
Msa0031370 MtrunA17_Chr7g0248721 47.887 71 35 1 225 295 128 196 5.25e-16 77.0
Msa0031370 MtrunA17_Chr5g0418781 45.946 74 39 1 225 298 111 183 4.59e-15 75.5
Msa0031370 MtrunA17_Chr5g0443131 46.479 71 37 1 225 295 144 213 4.78e-15 75.9
Msa0031370 MtrunA17_Chr8g0386241 44.595 74 40 1 222 295 87 159 6.82e-15 72.8
Msa0031370 MtrunA17_Chr7g0238921 56.897 58 24 1 238 295 124 180 1.84e-14 74.3
Msa0031370 MtrunA17_Chr4g0074001 38.462 104 62 2 201 303 321 423 4.83e-14 74.3
Msa0031370 MtrunA17_Chr7g0270831 38.235 102 57 3 199 295 173 273 1.04e-13 73.2
Msa0031370 MtrunA17_Chr3g0139021 40.594 101 47 4 196 295 283 371 3.98e-13 70.9
Msa0031370 MtrunA17_Chr7g0228941 49.254 67 32 2 235 300 49 114 4.37e-13 66.2
Msa0031370 MtrunA17_Chr2g0322391 42.466 73 40 2 225 296 336 407 4.85e-13 71.6
Msa0031370 MtrunA17_Chr4g0075201 43.056 72 39 2 225 295 254 324 6.74e-13 70.9
Msa0031370 MtrunA17_Chr8g0348311 39.216 102 45 4 195 295 127 212 7.64e-13 69.7
Msa0031370 MtrunA17_Chr1g0147881 53.226 62 27 2 235 295 259 319 8.89e-13 69.3
Msa0031370 MtrunA17_Chr2g0288871 43.056 72 39 2 225 295 292 362 9.03e-13 70.5
Msa0031370 MtrunA17_Chr3g0099641 42.466 73 40 2 225 296 238 309 9.99e-13 70.1
Msa0031370 MtrunA17_Chr2g0302391 27.604 192 127 6 114 295 79 268 1.25e-12 69.7
Msa0031370 MtrunA17_Chr2g0284321 54.237 59 25 2 239 296 36 93 2.44e-12 66.2
Msa0031370 MtrunA17_Chr4g0025061 44.444 72 38 2 225 295 347 417 3.32e-12 68.9
Msa0031370 MtrunA17_Chr8g0387501 40.404 99 49 3 237 334 128 217 7.42e-12 65.9
Msa0031370 MtrunA17_Chr1g0150281 40.000 75 41 3 225 296 125 198 1.20e-11 65.5
Msa0031370 MtrunA17_Chr7g0270051 51.724 58 26 2 239 295 54 110 1.24e-11 65.1
Msa0031370 MtrunA17_Chr2g0333851 40.541 74 40 3 225 295 154 226 1.27e-11 65.9
Msa0031370 MtrunA17_Chr5g0401821 47.368 76 34 3 225 295 182 256 1.47e-11 65.1
Msa0031370 MtrunA17_Chr1g0184691 49.231 65 31 2 235 298 290 353 1.55e-11 65.9
Msa0031370 MtrunA17_Chr4g0071521 46.970 66 33 2 235 299 242 306 1.74e-11 65.5
Msa0031370 MtrunA17_Chr4g0060271 41.096 73 41 2 225 296 242 313 1.90e-11 66.6
Msa0031370 MtrunA17_Chr7g0216891 43.056 72 39 2 225 295 206 276 3.54e-11 65.5
Msa0031370 MtrunA17_Chr4g0053281 49.206 63 30 2 235 296 263 324 4.32e-11 64.3
Msa0031370 MtrunA17_Chr2g0293481 45.455 66 34 2 231 295 260 324 5.05e-11 64.7
Msa0031370 MtrunA17_Chr7g0248691 41.304 92 44 5 234 324 150 232 5.30e-11 63.9
Msa0031370 MtrunA17_Chr2g0319961 53.448 58 25 2 239 295 81 137 7.35e-11 62.8
Msa0031370 MtrunA17_Chr4g0025051 41.558 77 43 2 220 295 317 392 8.81e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0031370 AT5G64810.1 39.806 103 61 1 193 295 65 166 2.14e-22 95.1
Msa0031370 AT1G64000.1 55.556 72 31 1 224 295 100 170 4.54e-21 91.3
Msa0031370 AT2G38470.1 34.444 180 101 5 168 335 293 467 1.97e-20 94.7
Msa0031370 AT5G41570.1 54.930 71 31 1 225 295 85 154 2.50e-20 89.0
Msa0031370 AT4G26640.7 54.930 71 31 1 225 295 328 397 2.91e-20 94.0
Msa0031370 AT4G26640.1 54.930 71 31 1 225 295 296 365 3.47e-20 93.6
Msa0031370 AT5G07100.2 52.439 82 31 2 225 299 128 208 3.54e-20 89.4
Msa0031370 AT5G07100.4 52.439 82 31 2 225 299 128 208 3.54e-20 89.4
Msa0031370 AT5G07100.5 52.439 82 31 2 225 299 128 208 3.54e-20 89.4
Msa0031370 AT5G07100.3 52.439 82 31 2 225 299 128 208 3.54e-20 89.4
Msa0031370 AT4G26640.2 54.930 71 31 1 225 295 368 437 3.54e-20 94.0
Msa0031370 AT5G07100.1 52.439 82 31 2 225 299 221 301 3.67e-20 91.7
Msa0031370 AT4G26640.5 54.930 71 31 1 225 295 192 261 4.17e-20 92.4
Msa0031370 AT4G26640.6 54.930 71 31 1 225 295 192 261 4.17e-20 92.4
Msa0031370 AT4G26640.4 54.930 71 31 1 225 295 192 261 4.17e-20 92.4
Msa0031370 AT4G26640.3 54.930 71 31 1 225 295 192 261 4.17e-20 92.4
Msa0031370 AT2G03340.1 54.930 71 31 1 225 295 402 471 7.07e-20 92.8
Msa0031370 AT1G13960.1 54.930 71 31 1 225 295 396 465 7.42e-20 92.8
Msa0031370 AT3G01080.2 54.930 71 31 1 225 295 331 400 1.30e-19 91.7
Msa0031370 AT1G13960.2 54.930 71 31 1 225 295 243 312 1.53e-19 90.5
Msa0031370 AT3G01080.1 54.930 71 31 1 225 295 293 362 2.12e-19 90.9
Msa0031370 AT4G26440.1 57.746 71 29 1 225 295 359 428 3.70e-19 90.9
Msa0031370 AT5G56270.1 54.930 71 31 1 225 295 474 543 5.09e-19 90.9
Msa0031370 AT1G55600.1 52.632 76 35 1 220 295 289 363 1.65e-18 88.6
Msa0031370 AT2G04880.2 38.017 121 74 1 175 295 220 339 2.84e-18 87.8
Msa0031370 AT2G04880.1 38.017 121 74 1 175 295 244 363 4.03e-18 87.4
Msa0031370 AT2G44745.1 47.500 80 41 1 225 304 132 210 8.89e-18 82.8
Msa0031370 AT2G30250.1 52.113 71 33 1 225 295 315 384 9.37e-18 85.5
Msa0031370 AT5G26170.1 43.243 74 41 1 225 298 100 172 1.82e-17 80.5
Msa0031370 AT2G46130.1 52.113 71 33 1 225 295 17 86 2.38e-17 78.2
Msa0031370 AT5G49520.1 52.113 71 33 1 225 295 208 277 2.49e-17 84.3
Msa0031370 AT4G39410.1 49.315 73 36 1 225 297 210 281 2.94e-17 82.8
Msa0031370 AT2G47260.1 49.296 71 35 1 225 295 161 230 3.71e-17 83.2
Msa0031370 AT1G29860.1 49.296 71 35 1 225 295 123 192 5.05e-17 81.6
Msa0031370 AT4G18170.1 47.887 71 36 1 225 295 159 228 2.69e-16 80.1
Msa0031370 AT1G69310.4 45.946 74 39 1 225 298 134 206 5.50e-16 79.0
Msa0031370 AT1G69310.2 45.946 74 39 1 225 298 134 206 5.50e-16 79.0
Msa0031370 AT1G69310.1 45.946 74 39 1 225 298 134 206 5.50e-16 79.0
Msa0031370 AT3G62340.1 52.113 71 33 1 225 295 105 174 5.95e-16 78.6
Msa0031370 AT5G13080.1 48.611 72 36 1 224 295 53 123 2.11e-15 73.9
Msa0031370 AT5G46350.1 45.070 71 38 1 225 295 170 239 2.29e-15 77.4
Msa0031370 AT2G21900.1 51.562 64 29 2 233 295 103 165 2.55e-15 75.1
Msa0031370 AT3G01970.1 48.052 77 37 2 225 301 52 125 2.64e-15 73.6
Msa0031370 AT4G31550.2 37.903 124 58 5 193 315 211 316 4.08e-15 76.6
Msa0031370 AT2G46130.2 47.887 71 32 1 225 295 17 82 1.03e-14 70.5
Msa0031370 AT1G69310.3 41.892 74 38 1 225 298 134 202 8.51e-14 72.4
Msa0031370 AT4G30935.1 39.048 105 58 3 201 303 295 395 9.07e-14 73.9
Msa0031370 AT4G31550.1 37.600 125 58 6 193 315 211 317 1.42e-13 72.0
Msa0031370 AT2G37260.1 49.206 63 31 1 233 295 344 405 2.72e-13 72.0
Msa0031370 AT2G37260.3 49.206 63 31 1 233 295 264 325 2.98e-13 71.6
Msa0031370 AT2G37260.2 49.206 63 31 1 233 295 262 323 3.21e-13 71.2
Msa0031370 AT4G04450.2 44.444 72 38 2 225 295 185 255 1.04e-12 70.5
Msa0031370 AT4G23550.1 43.836 73 39 2 224 295 126 197 1.04e-12 69.3
Msa0031370 AT5G52830.1 38.889 108 58 3 224 330 151 251 1.17e-12 69.7
Msa0031370 AT4G01250.1 48.000 75 36 3 222 295 113 185 1.51e-12 68.6
Msa0031370 AT2G25000.2 34.862 109 55 5 204 297 56 163 1.99e-12 67.4
Msa0031370 AT2G25000.4 34.862 109 55 5 204 297 56 163 1.99e-12 67.4
Msa0031370 AT4G24240.1 44.444 81 39 3 225 300 264 343 2.46e-12 68.6
Msa0031370 AT4G04450.1 44.444 72 38 2 225 295 279 349 2.63e-12 69.3
Msa0031370 AT5G15130.2 30.519 154 81 6 167 295 90 242 2.66e-12 69.3
Msa0031370 AT2G25000.3 34.862 109 55 5 204 297 78 185 3.17e-12 67.0
Msa0031370 AT5G15130.1 30.519 154 81 6 167 295 132 284 3.55e-12 68.9
Msa0031370 AT1G29280.1 53.448 58 25 2 239 295 75 131 3.63e-12 67.0
Msa0031370 AT4G22070.4 43.056 72 39 2 225 295 284 354 4.39e-12 68.9
Msa0031370 AT4G22070.3 43.056 72 39 2 225 295 284 354 4.39e-12 68.9
Msa0031370 AT4G22070.2 43.056 72 39 2 225 295 284 354 4.39e-12 68.9
Msa0031370 AT4G22070.1 43.056 72 39 2 225 295 284 354 4.39e-12 68.9
Msa0031370 AT2G46400.1 45.205 73 36 2 225 294 91 162 4.50e-12 67.4
Msa0031370 AT2G25000.1 34.862 109 55 5 204 297 99 206 4.91e-12 67.0
Msa0031370 AT2G23320.1 43.750 80 39 3 222 296 220 298 5.14e-12 67.4
Msa0031370 AT1G62300.1 43.056 72 39 2 225 295 299 369 7.17e-12 68.2
Msa0031370 AT4G01720.1 44.444 72 38 2 225 295 226 296 1.01e-11 67.4
Msa0031370 AT5G43290.1 43.056 72 39 2 225 295 100 170 1.26e-11 65.5
Msa0031370 AT2G24570.1 40.000 105 49 5 193 295 208 300 1.70e-11 65.9
Msa0031370 AT2G34830.1 47.692 65 32 2 232 295 209 272 3.27e-11 65.5
Msa0031370 AT2G34830.2 47.692 65 32 2 232 295 218 281 3.78e-11 65.5
Msa0031370 AT4G31800.2 46.032 63 31 3 237 297 110 171 7.23e-11 62.8
Msa0031370 AT1G30650.1 47.692 65 32 2 232 295 211 274 8.16e-11 64.3
Msa0031370 AT5G28650.1 43.478 69 37 2 235 302 259 326 8.89e-11 63.5
Msa0031370 AT4G31800.1 46.032 63 31 3 237 297 175 236 9.00e-11 63.5

Find 124 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCAAGTCTTTCCTTTCA+TGG 0.213204 1_1:+58104474 Msa0031370:CDS
TGAAAGTGATGAAGACATTT+TGG 0.222211 1_1:+58103056 Msa0031370:CDS
ATCAGTTGTACTATCTTCTT+TGG 0.230642 1_1:-58102703 None:intergenic
AATAATTACCTACGATTGTT+AGG 0.259815 1_1:-58103129 None:intergenic
GGAATTGGCCCGTTGATATT+TGG 0.273233 1_1:-58104268 None:intergenic
AATGAATCTCCTCCTTCTTC+TGG 0.277307 1_1:+58101287 Msa0031370:CDS
TATGTAACAAGTACATATTT+TGG 0.310515 1_1:-58103920 None:intergenic
TTTCTTCAGGTTGGTGGAAA+TGG 0.328210 1_1:+58104442 Msa0031370:CDS
GTTGTTGTTGTTGGTGTTGT+TGG 0.335406 1_1:-58101189 None:intergenic
AAAGGCCGCGTCATGGTATT+AGG 0.337576 1_1:-58104139 None:intergenic
TTACCCACCATGTTTAATAA+AGG 0.347175 1_1:-58104157 None:intergenic
GGGTGCTTCCCCAGCTGATC+AGG 0.355314 1_1:+58104495 Msa0031370:CDS
ACCACCATCTGCATTGTCTA+TGG 0.359566 1_1:+58104054 Msa0031370:CDS
GTCCTTGATCAGGTGGTGTT+GGG 0.367401 1_1:-58102560 None:intergenic
AACTCCTATTGATACAAATC+AGG 0.367991 1_1:+58104240 Msa0031370:CDS
ATACCTTATGGTATGAATTA+CGG 0.368502 1_1:+58104112 Msa0031370:CDS
CAATATGGCTATATACCTTA+TGG 0.368992 1_1:+58104100 Msa0031370:CDS
CATAAGGTATATAGCCATAT+TGG 0.374743 1_1:-58104099 None:intergenic
GCTCAAGTCTTTCCTTTCAT+GGG 0.380582 1_1:+58104475 Msa0031370:CDS
TCGAGAAATATTAATGCATC+TGG 0.384031 1_1:+58103995 Msa0031370:CDS
GGAAATACAGGAAGCGGAAT+TGG 0.386630 1_1:-58104283 None:intergenic
CCTAAATATCATTCGGTGTC+AGG 0.391994 1_1:-58104532 None:intergenic
TGCAATTCAGGAAGAGAAAG+AGG 0.396225 1_1:+58102965 Msa0031370:intron
CCATTTCACATTGTACCATA+TGG 0.401565 1_1:+58104412 Msa0031370:CDS
TATGTACTTGTTACATATGA+TGG 0.402941 1_1:+58103926 Msa0031370:CDS
CCAACTTGAGTTGCAGTCAT+AGG 0.404757 1_1:-58104331 None:intergenic
TGTTCACTTATGGGTCTGTA+AGG 0.409849 1_1:-58104193 None:intergenic
TCTGCACCATGTCCTTGATC+AGG 0.410286 1_1:-58102570 None:intergenic
CAAGTTGGACATAATATCAA+TGG 0.426656 1_1:+58104346 Msa0031370:CDS
CCAACCTGATTTGTATCAAT+AGG 0.427285 1_1:-58104244 None:intergenic
GGTGGTAATGGTGGTGATAT+CGG 0.428089 1_1:-58104304 None:intergenic
GGTGGTAATGGTGGTGATAT+CGG 0.428089 1_1:-58104391 None:intergenic
TCCGTAGCTTCCTGATCAGC+TGG 0.428219 1_1:-58104505 None:intergenic
AAGTGATTATAAAACCTTTG+TGG 0.432121 1_1:-58102849 None:intergenic
GTTGTTGGTGTTGTTGGTGT+TGG 0.432444 1_1:-58101183 None:intergenic
GTTGGTGTTGTTGGTGTTGG+TGG 0.433999 1_1:-58101180 None:intergenic
GGTGGTGATATCGGAAATAC+AGG 0.454748 1_1:-58104295 None:intergenic
GGTGGTGATATCGGAAATAC+AGG 0.454748 1_1:-58104382 None:intergenic
CAAGTATCGAACACTGAAGT+TGG 0.470605 1_1:+58101221 Msa0031370:CDS
TGAGTTGCAGTCATAGGAAA+TGG 0.474839 1_1:-58104325 None:intergenic
ACTGCTTCAGAAAAGTTCAT+TGG 0.480463 1_1:-58102675 None:intergenic
CAACCGTAATTCATACCATA+AGG 0.481218 1_1:-58104115 None:intergenic
CCTATGACTGCAACTCAAGT+TGG 0.482132 1_1:+58104331 Msa0031370:CDS
TGTCCTTGATCAGGTGGTGT+TGG 0.482577 1_1:-58102561 None:intergenic
ATATAGCCATATTGGTGAAA+AGG 0.483202 1_1:-58104091 None:intergenic
TGAAGAAAATCCCCACCATA+TGG 0.492962 1_1:-58104427 None:intergenic
TTAATATTTCTCGAGTATGT+TGG 0.504174 1_1:-58103986 None:intergenic
GTTGAACTTCAGTTTCAGCT+TGG 0.505017 1_1:-58102515 None:intergenic
GATGATGAAGCAACTGCCAT+TGG 0.509238 1_1:+58102777 Msa0031370:CDS
TATGTAGCCTAAATATCATT+CGG 0.509419 1_1:-58104539 None:intergenic
CTTGCCCCATGACAGATGTC+GGG 0.511254 1_1:+58103013 Msa0031370:CDS
CCGACATCTGTCATGGGGCA+AGG 0.512302 1_1:-58103012 None:intergenic
AGGTTGGACCAAATATCAAC+GGG 0.512849 1_1:+58104260 Msa0031370:CDS
CACACATATGTCTAATACCA+TGG 0.515029 1_1:+58101148 None:intergenic
CATCTCCTCTTGTTGCCACA+TGG 0.526145 1_1:-58102485 None:intergenic
TTCACATTGTACCATATGGT+GGG 0.526608 1_1:+58104416 Msa0031370:CDS
CCATATGGTACAATGTGAAA+TGG 0.527122 1_1:-58104412 None:intergenic
GCTGGGGAAGCACCCATGAA+AGG 0.527399 1_1:-58104487 None:intergenic
CCCAGCTGATCAGGAAGCTA+CGG 0.531217 1_1:+58104504 Msa0031370:CDS
CCTTGCCCCATGACAGATGT+CGG 0.532507 1_1:+58103012 Msa0031370:CDS
AAGTTGGACATAATATCAAT+GGG 0.540013 1_1:+58104347 Msa0031370:CDS
TCCCCAACACCACCTGATCA+AGG 0.540123 1_1:+58102558 Msa0031370:CDS
TATGATGATGGTGGTGCTAG+TGG 0.544707 1_1:+58102819 Msa0031370:CDS
GTCATAGGAAATGGTGGTAA+TGG 0.551917 1_1:-58104316 None:intergenic
GATGAAGCAACTGCCATTGG+AGG 0.552119 1_1:+58102780 Msa0031370:CDS
GGTTATTGTTGGCGCAAGTA+CGG 0.553147 1_1:+58103084 Msa0031370:CDS
AAATTCTAAGGGCAAGGAAA+GGG 0.559579 1_1:+58102646 Msa0031370:CDS
TCACATTGTACCATATGGTG+GGG 0.559672 1_1:+58104417 Msa0031370:CDS
ACAATGTGAAATGGTGGTAA+TGG 0.559807 1_1:-58104403 None:intergenic
GAAATTCTAAGGGCAAGGAA+AGG 0.560612 1_1:+58102645 Msa0031370:CDS
ATGCTGAAATTCTAAGGGCA+AGG 0.561694 1_1:+58102640 Msa0031370:CDS
CCGTAGCTTCCTGATCAGCT+GGG 0.562306 1_1:-58104504 None:intergenic
TCCTTCTTCTGGTTATTCCG+AGG 0.562652 1_1:+58101298 Msa0031370:CDS
ACCTCGGAATAACCAGAAGA+AGG 0.563675 1_1:-58101299 None:intergenic
GATATCGGAAATACAGGAAG+CGG 0.565608 1_1:-58104289 None:intergenic
TATTGGTGAAAAGGAGGGAG+AGG 0.567707 1_1:-58104082 None:intergenic
CAGCAACATCATTCCTCCAA+TGG 0.568839 1_1:-58102793 None:intergenic
TTTGTCAATGACAGGTCCGT+GGG 0.568911 1_1:-58103957 None:intergenic
TTTCACATTGTACCATATGG+TGG 0.572755 1_1:+58104415 Msa0031370:CDS
CCTATTGATACAAATCAGGT+TGG 0.576330 1_1:+58104244 Msa0031370:CDS
GCAATTCAGGAAGAGAAAGA+GGG 0.576835 1_1:+58102966 Msa0031370:intron
TAGCCATATTGGTGAAAAGG+AGG 0.582097 1_1:-58104088 None:intergenic
TCCTTGATCAGGTGGTGTTG+GGG 0.585344 1_1:-58102559 None:intergenic
GTTTAATAAAGGCCGCGTCA+TGG 0.585854 1_1:-58104146 None:intergenic
GCAGTTGCTTCATCATCCGC+AGG 0.586772 1_1:-58102771 None:intergenic
GCAGATGGTGGTGATGGTGG+TGG 0.586837 1_1:-58104043 None:intergenic
TGTCTTCATCACTTTCAACT+CGG 0.590497 1_1:-58103050 None:intergenic
CTTATGGTATGAATTACGGT+TGG 0.590836 1_1:+58104116 Msa0031370:CDS
TGATGGTGGTGGCAGCATGT+TGG 0.595809 1_1:-58104032 None:intergenic
CGGCCTTTATTAAACATGGT+GGG 0.600743 1_1:+58104154 Msa0031370:CDS
CAGGTTGGACCAAATATCAA+CGG 0.601960 1_1:+58104259 Msa0031370:CDS
TACGGATGCAAAGAGATCAA+AGG 0.609552 1_1:+58103102 Msa0031370:CDS
ATGTGAAATGGTGGTAATGG+TGG 0.612583 1_1:-58104400 None:intergenic
GCACCATGTCCTTGATCAGG+TGG 0.613784 1_1:-58102567 None:intergenic
GACGCGGCCTTTATTAAACA+TGG 0.614916 1_1:+58104150 Msa0031370:CDS
TCTGAAACTTGATCATCAGT+TGG 0.615285 1_1:-58102744 None:intergenic
AAGGAAATCCTAACAATCGT+AGG 0.617006 1_1:+58103121 Msa0031370:CDS
TTTGCCCGACATCTGTCATG+GGG 0.617609 1_1:-58103017 None:intergenic
GACAATGCAGATGGTGGTGA+TGG 0.620508 1_1:-58104049 None:intergenic
ATGTTGCTGCAATATGATGA+TGG 0.628176 1_1:+58102807 Msa0031370:CDS
ATAGGAAATGGTGGTAATGG+TGG 0.629683 1_1:-58104313 None:intergenic
GCGGCCTTTATTAAACATGG+TGG 0.630164 1_1:+58104153 Msa0031370:CDS
AAGAGAGTATATAAATACCT+CGG 0.632254 1_1:-58101315 None:intergenic
ATTGGTGAAAAGGAGGGAGA+GGG 0.644988 1_1:-58104081 None:intergenic
GATTGCCATGTGGCAACAAG+AGG 0.647237 1_1:+58102480 Msa0031370:CDS
AATGCAGATGGTGGTGATGG+TGG 0.647565 1_1:-58104046 None:intergenic
CGTAGCTTCCTGATCAGCTG+GGG 0.653265 1_1:-58104503 None:intergenic
AGCCATATTGGTGAAAAGGA+GGG 0.654609 1_1:-58104087 None:intergenic
TATGATGGAAAACATACCCA+CGG 0.656713 1_1:+58103941 Msa0031370:CDS
GGGTCTGTAAGGTTGCATCA+TGG 0.657757 1_1:-58104182 None:intergenic
TGAACCATAGACAATGCAGA+TGG 0.660865 1_1:-58104058 None:intergenic
GTTGCAGTCATAGGAAATGG+TGG 0.663032 1_1:-58104322 None:intergenic
GCAAAATGATGCAAATGTAG+TGG 0.669148 1_1:+58102611 Msa0031370:CDS
CTAGTGGATCTTGTCCACAA+AGG 0.681150 1_1:+58102835 Msa0031370:CDS
GGGGATGAGAAATTCAACAC+TGG 0.684419 1_1:-58102540 None:intergenic
ACATCTGTCATGGGGCAAGG+TGG 0.686804 1_1:-58103009 None:intergenic
TCGGAATAACCAGAAGAAGG+AGG 0.688798 1_1:-58101296 None:intergenic
TTCTGCTGATGATTGCCATG+TGG 0.692089 1_1:+58102470 Msa0031370:CDS
TATGGTACAATGTGAAATGG+TGG 0.694153 1_1:-58104409 None:intergenic
ACCATAGACAATGCAGATGG+TGG 0.695551 1_1:-58104055 None:intergenic
ACACCACCTGATCAAGGACA+TGG 0.702867 1_1:+58102564 Msa0031370:CDS
TTGCTGCAATATGATGATGG+TGG 0.726113 1_1:+58102810 Msa0031370:CDS
TCAAGTTTCAGATACACCTG+CGG 0.763156 1_1:+58102755 Msa0031370:CDS
GTTGGCCTAATACCATGACG+CGG 0.807585 1_1:+58104134 Msa0031370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCATCAAAAAATAAAA+AGG - chr1_1:58101601-58101620 None:intergenic 10.0%
!!! AATGGTTTTTCAATTAAATT+CGG - chr1_1:58101853-58101872 None:intergenic 15.0%
!! AAGTGACATTATTTAAAAAA+GGG - chr1_1:58101909-58101928 None:intergenic 15.0%
!! AAAGTGACATTATTTAAAAA+AGG - chr1_1:58101910-58101929 None:intergenic 15.0%
!!! TTTTGTTATTATAGTTTCAT+AGG + chr1_1:58102261-58102280 Msa0031370:intron 15.0%
!! TTGTAAAAATATCAAAAACA+AGG - chr1_1:58103432-58103451 None:intergenic 15.0%
!! CAAATTTAATATCAAATTCT+AGG - chr1_1:58103589-58103608 None:intergenic 15.0%
!! AGAATTTGATATTAAATTTG+TGG + chr1_1:58103589-58103608 Msa0031370:intron 15.0%
!!! CTTTATCATTTTATTTTAAG+CGG + chr1_1:58103659-58103678 Msa0031370:intron 15.0%
!! TACTAATACATTTATGTAAA+TGG - chr1_1:58103763-58103782 None:intergenic 15.0%
!! TCACACAATATTAAATAATT+AGG - chr1_1:58103826-58103845 None:intergenic 15.0%
!! TCTTCATTCAAGATTTAAAA+TGG - chr1_1:58101871-58101890 None:intergenic 20.0%
!!! GAATTTGGATTTGTTTATAT+TGG - chr1_1:58101979-58101998 None:intergenic 20.0%
!! ATATTCCTAATGAGAAATAA+TGG - chr1_1:58102058-58102077 None:intergenic 20.0%
!! ATAAATAAATCATTCCATGT+TGG - chr1_1:58102315-58102334 None:intergenic 20.0%
!! GTAATTGAATCAAATTCATT+AGG + chr1_1:58102446-58102465 Msa0031370:intron 20.0%
!! ACATTGTTGCTAATAAATAA+AGG + chr1_1:58102917-58102936 Msa0031370:intron 20.0%
!!! TTTTACGATAAATCACTTAT+CGG - chr1_1:58103562-58103581 None:intergenic 20.0%
!! TTTAATATTGTGTGAATTGT+AGG + chr1_1:58103831-58103850 Msa0031370:intron 20.0%
!! TTAATATTGTGTGAATTGTA+GGG + chr1_1:58103832-58103851 Msa0031370:intron 20.0%
!!! TATGTAACAAGTACATATTT+TGG - chr1_1:58103923-58103942 None:intergenic 20.0%
!!! AGAAAATTTTGTTCACTTAT+GGG - chr1_1:58104205-58104224 None:intergenic 20.0%
!!! TAGAAAATTTTGTTCACTTA+TGG - chr1_1:58104206-58104225 None:intergenic 20.0%
!!! ATTTTCATTGTTGTTGTTGT+TGG - chr1_1:58101201-58101220 None:intergenic 25.0%
!! AAGAGAGTATATAAATACCT+CGG - chr1_1:58101318-58101337 None:intergenic 25.0%
! AAGTTGTATTAGTGCTAAAA+TGG - chr1_1:58102088-58102107 None:intergenic 25.0%
! TACATGTTAGATTGAATTGA+AGG - chr1_1:58102155-58102174 None:intergenic 25.0%
! TGAAAAATGCTGAAATTCTA+AGG + chr1_1:58102634-58102653 Msa0031370:CDS 25.0%
! GAAAAATGCTGAAATTCTAA+GGG + chr1_1:58102635-58102654 Msa0031370:CDS 25.0%
! AAGTGATTATAAAACCTTTG+TGG - chr1_1:58102852-58102871 None:intergenic 25.0%
! AATAATTACCTACGATTGTT+AGG - chr1_1:58103132-58103151 None:intergenic 25.0%
! TGCATTAACACATATAAAAG+AGG - chr1_1:58103157-58103176 None:intergenic 25.0%
! AATGACATAATGATCATTGA+CGG + chr1_1:58103370-58103389 Msa0031370:intron 25.0%
! AATCTAACAATGATTGATGT+TGG - chr1_1:58103499-58103518 None:intergenic 25.0%
!!! TAGAGATGATTTTCAAGTAT+TGG + chr1_1:58103519-58103538 Msa0031370:intron 25.0%
!!! AGAGATGATTTTCAAGTATT+GGG + chr1_1:58103520-58103539 Msa0031370:intron 25.0%
! TAATATTGTGTGAATTGTAG+GGG + chr1_1:58103833-58103852 Msa0031370:intron 25.0%
! TTGTGACATTAAAAAACAAG+TGG + chr1_1:58103880-58103899 Msa0031370:CDS 25.0%
! ACATTAAAAAACAAGTGGAA+AGG + chr1_1:58103885-58103904 Msa0031370:CDS 25.0%
! CATTAAAAAACAAGTGGAAA+GGG + chr1_1:58103886-58103905 Msa0031370:CDS 25.0%
!! TATGTACTTGTTACATATGA+TGG + chr1_1:58103926-58103945 Msa0031370:CDS 25.0%
! TTAATATTTCTCGAGTATGT+TGG - chr1_1:58103989-58104008 None:intergenic 25.0%
! ATACCTTATGGTATGAATTA+CGG + chr1_1:58104112-58104131 Msa0031370:CDS 25.0%
! AAGTTGGACATAATATCAAT+GGG + chr1_1:58104347-58104366 Msa0031370:CDS 25.0%
GGTGAAAAATGATGTGAATT+TGG - chr1_1:58101994-58102013 None:intergenic 30.0%
! ACTTTTAATAACCACACAAG+CGG - chr1_1:58102020-58102039 None:intergenic 30.0%
! ATGCACCATTATTTCTCATT+AGG + chr1_1:58102050-58102069 Msa0031370:intron 30.0%
ATTGAATTGAAGGTCTGATT+AGG - chr1_1:58102145-58102164 None:intergenic 30.0%
!! CAAATAGTTTTACACCAACA+TGG + chr1_1:58102298-58102317 Msa0031370:intron 30.0%
ATCAGTTGTACTATCTTCTT+TGG - chr1_1:58102706-58102725 None:intergenic 30.0%
!! GCTCAATTATTTTGCAATTC+AGG + chr1_1:58102953-58102972 Msa0031370:intron 30.0%
! TGAAAGTGATGAAGACATTT+TGG + chr1_1:58103056-58103075 Msa0031370:CDS 30.0%
!! TTTTGGATGATGGTTATTGT+TGG + chr1_1:58103073-58103092 Msa0031370:CDS 30.0%
!! TTTTCTTTTTCATTCACTCC+TGG + chr1_1:58103200-58103219 Msa0031370:intron 30.0%
GTCAATGATCATTATGTCAT+TGG - chr1_1:58103371-58103390 None:intergenic 30.0%
AATATTGTGTGAATTGTAGG+GGG + chr1_1:58103834-58103853 Msa0031370:intron 30.0%
TCGAGAAATATTAATGCATC+TGG + chr1_1:58103995-58104014 Msa0031370:CDS 30.0%
ATATAGCCATATTGGTGAAA+AGG - chr1_1:58104094-58104113 None:intergenic 30.0%
CATAAGGTATATAGCCATAT+TGG - chr1_1:58104102-58104121 None:intergenic 30.0%
CAATATGGCTATATACCTTA+TGG + chr1_1:58104100-58104119 Msa0031370:CDS 30.0%
TTACCCACCATGTTTAATAA+AGG - chr1_1:58104160-58104179 None:intergenic 30.0%
AACTCCTATTGATACAAATC+AGG + chr1_1:58104240-58104259 Msa0031370:CDS 30.0%
! CAAGTTGGACATAATATCAA+TGG + chr1_1:58104346-58104365 Msa0031370:CDS 30.0%
! CATTTGCATCATTTTGCACA+TGG - chr1_1:58102608-58102627 None:intergenic 35.0%
GCAAAATGATGCAAATGTAG+TGG + chr1_1:58102611-58102630 Msa0031370:CDS 35.0%
! AAATTCTAAGGGCAAGGAAA+GGG + chr1_1:58102646-58102665 Msa0031370:CDS 35.0%
ACTGCTTCAGAAAAGTTCAT+TGG - chr1_1:58102678-58102697 None:intergenic 35.0%
! TCTGAAACTTGATCATCAGT+TGG - chr1_1:58102747-58102766 None:intergenic 35.0%
ATGTTGCTGCAATATGATGA+TGG + chr1_1:58102807-58102826 Msa0031370:CDS 35.0%
TGTCTTCATCACTTTCAACT+CGG - chr1_1:58103053-58103072 None:intergenic 35.0%
! GATGAAGACATTTTGGATGA+TGG + chr1_1:58103063-58103082 Msa0031370:CDS 35.0%
AAGGAAATCCTAACAATCGT+AGG + chr1_1:58103121-58103140 Msa0031370:CDS 35.0%
TCCTCTCTATATATACACCA+AGG - chr1_1:58103226-58103245 None:intergenic 35.0%
TTGGTGTATATATAGAGAGG+AGG + chr1_1:58103225-58103244 Msa0031370:intron 35.0%
ACAACAGTGTTCATCATCAT+TGG - chr1_1:58103302-58103321 None:intergenic 35.0%
!! TTATGTCATTGGCTTAGTTG+TGG - chr1_1:58103360-58103379 None:intergenic 35.0%
!! TGATTGATGTTGGTTCAACA+CGG - chr1_1:58103489-58103508 None:intergenic 35.0%
! TATGATGGAAAACATACCCA+CGG + chr1_1:58103941-58103960 Msa0031370:CDS 35.0%
!!! GGTCTTTTTTTGTCAATGAC+AGG - chr1_1:58103968-58103987 None:intergenic 35.0%
CAACCGTAATTCATACCATA+AGG - chr1_1:58104118-58104137 None:intergenic 35.0%
CTTATGGTATGAATTACGGT+TGG + chr1_1:58104116-58104135 Msa0031370:CDS 35.0%
! CCAACCTGATTTGTATCAAT+AGG - chr1_1:58104247-58104266 None:intergenic 35.0%
CCTATTGATACAAATCAGGT+TGG + chr1_1:58104244-58104263 Msa0031370:CDS 35.0%
ACAATGTGAAATGGTGGTAA+TGG - chr1_1:58104406-58104425 None:intergenic 35.0%
TATGGTACAATGTGAAATGG+TGG - chr1_1:58104412-58104431 None:intergenic 35.0%
CCATATGGTACAATGTGAAA+TGG - chr1_1:58104415-58104434 None:intergenic 35.0%
CCATTTCACATTGTACCATA+TGG + chr1_1:58104412-58104431 Msa0031370:CDS 35.0%
TTTCACATTGTACCATATGG+TGG + chr1_1:58104415-58104434 Msa0031370:CDS 35.0%
TTCACATTGTACCATATGGT+GGG + chr1_1:58104416-58104435 Msa0031370:CDS 35.0%
!! GTTGTTGTTGTTGGTGTTGT+TGG - chr1_1:58101192-58101211 None:intergenic 40.0%
!! CAAGTATCGAACACTGAAGT+TGG + chr1_1:58101221-58101240 Msa0031370:CDS 40.0%
AATGAATCTCCTCCTTCTTC+TGG + chr1_1:58101287-58101306 Msa0031370:CDS 40.0%
AAATGAACCAGTAGCAACCA+CGG - chr1_1:58101777-58101796 None:intergenic 40.0%
GTTGAACTTCAGTTTCAGCT+TGG - chr1_1:58102518-58102537 None:intergenic 40.0%
! ATGCTGAAATTCTAAGGGCA+AGG + chr1_1:58102640-58102659 Msa0031370:CDS 40.0%
! GAAATTCTAAGGGCAAGGAA+AGG + chr1_1:58102645-58102664 Msa0031370:CDS 40.0%
TCAAGTTTCAGATACACCTG+CGG + chr1_1:58102755-58102774 Msa0031370:CDS 40.0%
!! TTGCTGCAATATGATGATGG+TGG + chr1_1:58102810-58102829 Msa0031370:CDS 40.0%
TGCAATTCAGGAAGAGAAAG+AGG + chr1_1:58102965-58102984 Msa0031370:intron 40.0%
GCAATTCAGGAAGAGAAAGA+GGG + chr1_1:58102966-58102985 Msa0031370:intron 40.0%
TACGGATGCAAAGAGATCAA+AGG + chr1_1:58103102-58103121 Msa0031370:CDS 40.0%
TCTATATATACACCAAGGCC+AGG - chr1_1:58103221-58103240 None:intergenic 40.0%
! GCCTTGGTGTATATATAGAG+AGG + chr1_1:58103222-58103241 Msa0031370:intron 40.0%
TTGACGGTTTGACGAAAACA+TGG + chr1_1:58103386-58103405 Msa0031370:intron 40.0%
TGACGGTTTGACGAAAACAT+GGG + chr1_1:58103387-58103406 Msa0031370:intron 40.0%
TGAACCATAGACAATGCAGA+TGG - chr1_1:58104061-58104080 None:intergenic 40.0%
AGCCATATTGGTGAAAAGGA+GGG - chr1_1:58104090-58104109 None:intergenic 40.0%
TAGCCATATTGGTGAAAAGG+AGG - chr1_1:58104091-58104110 None:intergenic 40.0%
CGGCCTTTATTAAACATGGT+GGG + chr1_1:58104154-58104173 Msa0031370:CDS 40.0%
TGTTCACTTATGGGTCTGTA+AGG - chr1_1:58104196-58104215 None:intergenic 40.0%
! CAGGTTGGACCAAATATCAA+CGG + chr1_1:58104259-58104278 Msa0031370:CDS 40.0%
AGGTTGGACCAAATATCAAC+GGG + chr1_1:58104260-58104279 Msa0031370:CDS 40.0%
GATATCGGAAATACAGGAAG+CGG - chr1_1:58104292-58104311 None:intergenic 40.0%
!! ATAGGAAATGGTGGTAATGG+TGG - chr1_1:58104316-58104335 None:intergenic 40.0%
GTCATAGGAAATGGTGGTAA+TGG - chr1_1:58104319-58104338 None:intergenic 40.0%
TGAGTTGCAGTCATAGGAAA+TGG - chr1_1:58104328-58104347 None:intergenic 40.0%
!! ATGTGAAATGGTGGTAATGG+TGG - chr1_1:58104403-58104422 None:intergenic 40.0%
TCACATTGTACCATATGGTG+GGG + chr1_1:58104417-58104436 Msa0031370:CDS 40.0%
TGAAGAAAATCCCCACCATA+TGG - chr1_1:58104430-58104449 None:intergenic 40.0%
!! ATATGGTGGGGATTTTCTTC+AGG + chr1_1:58104429-58104448 Msa0031370:CDS 40.0%
!! TTTCTTCAGGTTGGTGGAAA+TGG + chr1_1:58104442-58104461 Msa0031370:CDS 40.0%
TGCTCAAGTCTTTCCTTTCA+TGG + chr1_1:58104474-58104493 Msa0031370:CDS 40.0%
GCTCAAGTCTTTCCTTTCAT+GGG + chr1_1:58104475-58104494 Msa0031370:CDS 40.0%
!!! GTTGGTGGTGTTGTTTTCCA+TGG - chr1_1:58101168-58101187 None:intergenic 45.0%
!! GTTGTTGGTGTTGTTGGTGT+TGG - chr1_1:58101186-58101205 None:intergenic 45.0%
TCGGAATAACCAGAAGAAGG+AGG - chr1_1:58101299-58101318 None:intergenic 45.0%
! TCCTTCTTCTGGTTATTCCG+AGG + chr1_1:58101298-58101317 Msa0031370:CDS 45.0%
ACCTCGGAATAACCAGAAGA+AGG - chr1_1:58101302-58101321 None:intergenic 45.0%
TAATAACCACACAAGCGGAG+AGG - chr1_1:58102015-58102034 None:intergenic 45.0%
TTCTGCTGATGATTGCCATG+TGG + chr1_1:58102470-58102489 Msa0031370:CDS 45.0%
GGGGATGAGAAATTCAACAC+TGG - chr1_1:58102543-58102562 None:intergenic 45.0%
GATGATGAAGCAACTGCCAT+TGG + chr1_1:58102777-58102796 Msa0031370:CDS 45.0%
CAGCAACATCATTCCTCCAA+TGG - chr1_1:58102796-58102815 None:intergenic 45.0%
!! TATGATGATGGTGGTGCTAG+TGG + chr1_1:58102819-58102838 Msa0031370:CDS 45.0%
! CTAGTGGATCTTGTCCACAA+AGG + chr1_1:58102835-58102854 Msa0031370:CDS 45.0%
! TTTTGCCCGACATCTGTCAT+GGG - chr1_1:58103021-58103040 None:intergenic 45.0%
! TTTTTGCCCGACATCTGTCA+TGG - chr1_1:58103022-58103041 None:intergenic 45.0%
GGTTATTGTTGGCGCAAGTA+CGG + chr1_1:58103084-58103103 Msa0031370:CDS 45.0%
! TTTTCATTCACTCCTGGCCT+TGG + chr1_1:58103206-58103225 Msa0031370:intron 45.0%
TTTGTCAATGACAGGTCCGT+GGG - chr1_1:58103960-58103979 None:intergenic 45.0%
! TTTTGTCAATGACAGGTCCG+TGG - chr1_1:58103961-58103980 None:intergenic 45.0%
ACCACCATCTGCATTGTCTA+TGG + chr1_1:58104054-58104073 Msa0031370:CDS 45.0%
ACCATAGACAATGCAGATGG+TGG - chr1_1:58104058-58104077 None:intergenic 45.0%
ATTGGTGAAAAGGAGGGAGA+GGG - chr1_1:58104084-58104103 None:intergenic 45.0%
TATTGGTGAAAAGGAGGGAG+AGG - chr1_1:58104085-58104104 None:intergenic 45.0%
! CTCCCTCCTTTTCACCAATA+TGG + chr1_1:58104085-58104104 Msa0031370:CDS 45.0%
GTTTAATAAAGGCCGCGTCA+TGG - chr1_1:58104149-58104168 None:intergenic 45.0%
GACGCGGCCTTTATTAAACA+TGG + chr1_1:58104150-58104169 Msa0031370:CDS 45.0%
GCGGCCTTTATTAAACATGG+TGG + chr1_1:58104153-58104172 Msa0031370:CDS 45.0%
GGAATTGGCCCGTTGATATT+TGG - chr1_1:58104271-58104290 None:intergenic 45.0%
GGAAATACAGGAAGCGGAAT+TGG - chr1_1:58104286-58104305 None:intergenic 45.0%
GGTGGTGATATCGGAAATAC+AGG - chr1_1:58104298-58104317 None:intergenic 45.0%
!! GGTGGTAATGGTGGTGATAT+CGG - chr1_1:58104307-58104326 None:intergenic 45.0%
GTTGCAGTCATAGGAAATGG+TGG - chr1_1:58104325-58104344 None:intergenic 45.0%
! CCAACTTGAGTTGCAGTCAT+AGG - chr1_1:58104334-58104353 None:intergenic 45.0%
CCTATGACTGCAACTCAAGT+TGG + chr1_1:58104331-58104350 Msa0031370:CDS 45.0%
GGTGGTGATATCGGAAATAC+AGG - chr1_1:58104298-58104317 None:intergenic 45.0%
!! GGTGGTAATGGTGGTGATAT+CGG - chr1_1:58104307-58104326 None:intergenic 45.0%
!! TGTAATAATTATAATTTAAA+TGG - chr1_1:58102376-58102395 None:intergenic 5.0%
!! GTTGGTGTTGTTGGTGTTGG+TGG - chr1_1:58101183-58101202 None:intergenic 50.0%
! TTTTCACCTCTCCGCTTGTG+TGG + chr1_1:58102006-58102025 Msa0031370:intron 50.0%
GATTGCCATGTGGCAACAAG+AGG + chr1_1:58102480-58102499 Msa0031370:CDS 50.0%
CATCTCCTCTTGTTGCCACA+TGG - chr1_1:58102488-58102507 None:intergenic 50.0%
! TCCTTGATCAGGTGGTGTTG+GGG - chr1_1:58102562-58102581 None:intergenic 50.0%
GTCCTTGATCAGGTGGTGTT+GGG - chr1_1:58102563-58102582 None:intergenic 50.0%
TGTCCTTGATCAGGTGGTGT+TGG - chr1_1:58102564-58102583 None:intergenic 50.0%
ACACCACCTGATCAAGGACA+TGG + chr1_1:58102564-58102583 Msa0031370:CDS 50.0%
TCTGCACCATGTCCTTGATC+AGG - chr1_1:58102573-58102592 None:intergenic 50.0%
GATGAAGCAACTGCCATTGG+AGG + chr1_1:58102780-58102799 Msa0031370:CDS 50.0%
TTTGCCCGACATCTGTCATG+GGG - chr1_1:58103020-58103039 None:intergenic 50.0%
!! AATGCAGATGGTGGTGATGG+TGG - chr1_1:58104049-58104068 None:intergenic 50.0%
! GACAATGCAGATGGTGGTGA+TGG - chr1_1:58104052-58104071 None:intergenic 50.0%
! GTTGGCCTAATACCATGACG+CGG + chr1_1:58104134-58104153 Msa0031370:CDS 50.0%
AAAGGCCGCGTCATGGTATT+AGG - chr1_1:58104142-58104161 None:intergenic 50.0%
GGGTCTGTAAGGTTGCATCA+TGG - chr1_1:58104185-58104204 None:intergenic 50.0%
! GGTGGGGATTTTCTTCAGGT+TGG + chr1_1:58104433-58104452 Msa0031370:CDS 50.0%
!!! GGGGATTTTCTTCAGGTTGG+TGG + chr1_1:58104436-58104455 Msa0031370:CDS 50.0%
TCCCCAACACCACCTGATCA+AGG + chr1_1:58102558-58102577 Msa0031370:CDS 55.0%
GCACCATGTCCTTGATCAGG+TGG - chr1_1:58102570-58102589 None:intergenic 55.0%
GCAGTTGCTTCATCATCCGC+AGG - chr1_1:58102774-58102793 None:intergenic 55.0%
ACATCTGTCATGGGGCAAGG+TGG - chr1_1:58103012-58103031 None:intergenic 55.0%
CCTTGCCCCATGACAGATGT+CGG + chr1_1:58103012-58103031 Msa0031370:CDS 55.0%
CTTGCCCCATGACAGATGTC+GGG + chr1_1:58103013-58103032 Msa0031370:CDS 55.0%
!! TGATGGTGGTGGCAGCATGT+TGG - chr1_1:58104035-58104054 None:intergenic 55.0%
CGTAGCTTCCTGATCAGCTG+GGG - chr1_1:58104506-58104525 None:intergenic 55.0%
CCGTAGCTTCCTGATCAGCT+GGG - chr1_1:58104507-58104526 None:intergenic 55.0%
CCCAGCTGATCAGGAAGCTA+CGG + chr1_1:58104504-58104523 Msa0031370:CDS 55.0%
TCCGTAGCTTCCTGATCAGC+TGG - chr1_1:58104508-58104527 None:intergenic 55.0%
GTCGTGTGTGCGCGCATACA+CGG + chr1_1:58101751-58101770 Msa0031370:intron 60.0%
TACACGGCCGTGGTTGCTAC+TGG + chr1_1:58101767-58101786 Msa0031370:intron 60.0%
CCGACATCTGTCATGGGGCA+AGG - chr1_1:58103015-58103034 None:intergenic 60.0%
!! GCAGATGGTGGTGATGGTGG+TGG - chr1_1:58104046-58104065 None:intergenic 60.0%
!! GCTGGGGAAGCACCCATGAA+AGG - chr1_1:58104490-58104509 None:intergenic 60.0%
GGGTGCTTCCCCAGCTGATC+AGG + chr1_1:58104495-58104514 Msa0031370:CDS 65.0%
TGTGCGCGCATACACGGCCG+TGG + chr1_1:58101757-58101776 Msa0031370:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 58101155 58104546 58101155 ID=Msa0031370;Name=Msa0031370
chr1_1 mRNA 58101155 58104546 58101155 ID=Msa0031370-mRNA-1;Parent=Msa0031370;Name=Msa0031370-mRNA-1;_AED=0.02;_eAED=0.03;_QI=0|0|0|1|0.66|0.75|4|0|489
chr1_1 exon 58101155 58101373 58101155 ID=Msa0031370-mRNA-1:exon:17674;Parent=Msa0031370-mRNA-1
chr1_1 exon 58102468 58102856 58102468 ID=Msa0031370-mRNA-1:exon:17675;Parent=Msa0031370-mRNA-1
chr1_1 exon 58102975 58103142 58102975 ID=Msa0031370-mRNA-1:exon:17676;Parent=Msa0031370-mRNA-1
chr1_1 exon 58103853 58104546 58103853 ID=Msa0031370-mRNA-1:exon:17677;Parent=Msa0031370-mRNA-1
chr1_1 CDS 58101155 58101373 58101155 ID=Msa0031370-mRNA-1:cds;Parent=Msa0031370-mRNA-1
chr1_1 CDS 58102468 58102856 58102468 ID=Msa0031370-mRNA-1:cds;Parent=Msa0031370-mRNA-1
chr1_1 CDS 58102975 58103142 58102975 ID=Msa0031370-mRNA-1:cds;Parent=Msa0031370-mRNA-1
chr1_1 CDS 58103853 58104546 58103853 ID=Msa0031370-mRNA-1:cds;Parent=Msa0031370-mRNA-1
Gene Sequence

>Msa0031370

ATGTCTAATACCATGGAAAACAACACCACCAACACCAACAACACCAACAACAACAACAATGAAAATCAAGTATCGAACACTGAAGTTGGTTGCTCTGCTAAACAAAGTAAGAGAAGAGATTTGATCGATTTAAATGAATCTCCTCCTTCTTCTGGTTATTCCGAGGTATTTATATACTCTCTTTACTCTAATACATGTAAAGATGATGATCAAGATCATGCTTCTGCTGATGATTGCCATGTGGCAACAAGAGGAGATGTAAAACTCCAAGCTGAAACTGAAGTTCAACAACCAGTGTTGAATTTCTCATCCCCAACACCACCTGATCAAGGACATGGTGCAGAAGAAGAACAAGACCATGTGCAAAATGATGCAAATGTAGTGGTGAAAAATGCTGAAATTCTAAGGGCAAGGAAAGGGAAGATGCCAATGAACTTTTCTGAAGCAGTGCATGCCAAAGAAGATAGTACAACTGATGATAAAGAAACAAATGTACCAACTGATGATCAAGTTTCAGATACACCTGCGGATGATGAAGCAACTGCCATTGGAGGAATGATGTTGCTGCAATATGATGATGGTGGTGCTAGTGGATCTTGTCCACAAAGGAAGAGAAAGAGGGACTTCTCTGAATACTATAAACCACCTTGCCCCATGACAGATGTCGGGCAAAAAATAGTGATCCGAGTTGAAAGTGATGAAGACATTTTGGATGATGGTTATTGTTGGCGCAAGTACGGATGCAAAGAGATCAAAGGAAATCCTAACAATCGTAGGGGGTACTACAGATGCGCAACACACAATTGTGACATTAAAAAACAAGTGGAAAGGGATGCTAAAGATCCAAAATATGTACTTGTTACATATGATGGAAAACATACCCACGGACCTGTCATTGACAAAAAAAGACCAACATACTCGAGAAATATTAATGCATCTGGAGTTCGTAGAAATGCCAACATGCTGCCACCACCATCACCACCATCTGCATTGTCTATGGTTCACCCTCTCCCTCCTTTTCACCAATATGGCTATATACCTTATGGTATGAATTACGGTTGGCCTAATACCATGACGCGGCCTTTATTAAACATGGTGGGTAACACCATGATGCAACCTTACAGACCCATAAGTGAACAAAATTTTCTAGAACATCGTGCTTCAACTCCTATTGATACAAATCAGGTTGGACCAAATATCAACGGGCCAATTCCGCTTCCTGTATTTCCGATATCACCACCATTACCACCATTTCCTATGACTGCAACTCAAGTTGGACATAATATCAATGGGTCAGTTTCGCTTCCTGTATTTCCGATATCACCACCATTACCACCATTTCACATTGTACCATATGGTGGGGATTTTCTTCAGGTTGGTGGAAATGGTATTGTTGCTGCTCAAGTCTTTCCTTTCATGGGTGCTTCCCCAGCTGATCAGGAAGCTACGGATGTTCCTGACACCGAATGA
Protein sequence

>Msa0031370

MSNTMENNTTNTNNTNNNNNENQVSNTEVGCSAKQSKRRDLIDLNESPPSSGYSEVFIYSLYSNTCKDDDQDHASADDCHVATRGDVKLQAETEVQQPVLNFSSPTPPDQGHGAEEEQDHVQNDANVVVKNAEILRARKGKMPMNFSEAVHAKEDSTTDDKETNVPTDDQVSDTPADDEATAIGGMMLLQYDDGGASGSCPQRKRKRDFSEYYKPPCPMTDVGQKIVIRVESDEDILDDGYCWRKYGCKEIKGNPNNRRGYYRCATHNCDIKKQVERDAKDPKYVLVTYDGKHTHGPVIDKKRPTYSRNINASGVRRNANMLPPPSPPSALSMVHPLPPFHQYGYIPYGMNYGWPNTMTRPLLNMVGNTMMQPYRPISEQNFLEHRASTPIDTNQVGPNINGPIPLPVFPISPPLPPFPMTATQVGHNINGSVSLPVFPISPPLPPFHIVPYGGDFLQVGGNGIVAAQVFPFMGASPADQEATDVPDTE*