AlfalfaGEDB | Msa0032630

M. sativa cultivar zhongmu-4 / Msa0032630

KEGG: K09422
GO: GO:0000003 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006996 GO:0007033 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008285 GO:0009555 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009735 GO:0009739 GO:0009740 GO:0009751 GO:0009755 GO:0009787 GO:0009789 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022412 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033993 GO:0040008 GO:0042127 GO:0042221 GO:0042493 GO:0043067 GO:0043068 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0044703 GO:0045893 GO:0045926 GO:0045935 GO:0046677 GO:0048229 GO:0048232 GO:0048235 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048466 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048609 GO:0048653 GO:0048654 GO:0048655 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051716 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071840 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1905957 GO:1905959 GO:1990019 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032630	sp\|Q8W1W6\|MYB33_ARATH	65.060	83	29	0	6	88	28	110	2.59e-33	124

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0032630	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032630	MtrunA17_Chr1g0192411	83.516	91	15	2	92	4	94	2.77e-52	169
Msa0032630	MtrunA17_Chr8g0354661	65.823	79	27	10	88	37	115	1.64e-32	119
Msa0032630	MtrunA17_Chr3g0099141	60.241	83	33	6	88	36	118	9.06e-32	109
Msa0032630	MtrunA17_Chr3g0099131	63.291	79	29	10	88	41	119	1.55e-31	117
Msa0032630	MtrunA17_Chr1g0154031	62.025	79	30	10	88	76	154	4.43e-31	114
Msa0032630	MtrunA17_Chr3g0080861	59.494	79	32	10	88	37	115	1.36e-30	114
Msa0032630	MtrunA17_Chr2g0322521	61.728	81	31	8	88	27	107	7.25e-29	108
Msa0032630	MtrunA17_Chr1g0187761	51.899	79	38	10	88	12	90	2.18e-25	96.7
Msa0032630	MtrunA17_Chr7g0274581	47.619	84	44	10	93	12	95	6.31e-25	94.4
Msa0032630	MtrunA17_Chr8g0346561	50.000	84	42	10	93	12	95	6.85e-25	96.3

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032630	AT5G06100.1	65.060	83	29	6	88	28	110	1.22e-34	124
Msa0032630	AT5G06100.5	65.060	83	29	6	88	28	110	2.63e-34	124
Msa0032630	AT5G06100.2	65.060	83	29	6	88	28	110	2.63e-34	124
Msa0032630	AT5G06100.3	65.060	83	29	6	88	28	110	2.63e-34	124
Msa0032630	AT5G06100.4	65.060	83	29	6	88	28	110	2.63e-34	124
Msa0032630	AT2G32460.2	64.557	79	28	10	88	6	84	7.70e-34	122
Msa0032630	AT2G32460.1	64.557	79	28	10	88	18	96	8.66e-34	122
Msa0032630	AT3G11440.2	64.557	79	28	10	88	41	119	1.31e-33	122
Msa0032630	AT3G11440.3	64.557	79	28	10	88	41	119	2.65e-33	122
Msa0032630	AT3G11440.4	64.557	79	28	10	88	41	119	2.65e-33	122

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Find 26 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATATGTGAGAAATTA+TGG	0.216509	1_1:+59808030	Msa0032630:CDS
AAATTATGGAGAGGGTAATT+GGG	0.400713	1_1:+59808044	Msa0032630:CDS
AACAAATATATCCTATGGTT+AGG	0.447208	1_1:+59808270	Msa0032630:CDS
AAGGAGGACTGTATATCCTA+TGG	0.529147	1_1:+59808233	Msa0032630:CDS
AATAAGCAATTCCTAACCAT+AGG	0.585656	1_1:-59808281	None:intergenic
AATGGTCTCTATGGTTAAGG+AGG	0.630284	1_1:+59808217	Msa0032630:CDS
AGACCATTTGGGTCCGATCT+TGG	0.400185	1_1:-59808202	None:intergenic
AGGACCATGGTCATTACATG+AGG	0.640554	1_1:+59807996	Msa0032630:CDS
ATATGTGAGAAATTATGGAG+AGG	0.669714	1_1:+59808035	Msa0032630:CDS
ATGCCAAGATCGGACCCAAA+TGG	0.560899	1_1:+59808199	Msa0032630:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATGTGAGAAATTATGGAG+AGG	+	chr1_1:59808035-59808054	Msa0032630:CDS	30.0%
TATGTGAGAAATTATGGAGA+GGG	+	chr1_1:59808036-59808055	Msa0032630:CDS	30.0%
AAATTATGGAGAGGGTAATT+GGG	+	chr1_1:59808044-59808063	Msa0032630:CDS	30.0%
TTTACCACATCGAAAAAGTT+CGG	-	chr1_1:59808088-59808107	None:intergenic	30.0%
CCTACATCCTAATTTAGAAA+AGG	+	chr1_1:59808134-59808153	Msa0032630:CDS	30.0%
TGTTCTTCTTCTCTAGTAAT+TGG	-	chr1_1:59808162-59808181	None:intergenic	30.0%
AATAAGCAATTCCTAACCAT+AGG	-	chr1_1:59808284-59808303	None:intergenic	30.0%
ATGGTTAGGAATTGCTTATT+TGG	+	chr1_1:59808284-59808303	Msa0032630:CDS	30.0%
GAAATTATGGAGAGGGTAAT+TGG	+	chr1_1:59808043-59808062	Msa0032630:CDS	35.0%
GTAAAAGTTGTCGTCTAAGA+TGG	+	chr1_1:59808103-59808122	Msa0032630:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	59807961	59808356	59807961	ID=Msa0032630;Name=Msa0032630
chr1_1	mRNA	59807961	59808356	59807961	ID=Msa0032630-mRNA-1;Parent=Msa0032630;Name=Msa0032630-mRNA-1;_AED=0.55;_eAED=0.55;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|131
chr1_1	exon	59807961	59808356	59807961	ID=Msa0032630-mRNA-1:exon:18601;Parent=Msa0032630-mRNA-1
chr1_1	CDS	59807961	59808356	59807961	ID=Msa0032630-mRNA-1:cds;Parent=Msa0032630-mRNA-1

Gene Sequence

>Msa0032630

ATGTCGTACGGTGCTGATACTACTAGGTTGAAAAAAGGACCATGGTCATTACATGAGGATGCAATATTAAAAGAATATGTGAGAAATTATGGAGAGGGTAATTGGGATGCGGTAAAAGAGAAAACCGAACTTTTTCGATGTGGTAAAAGTTGTCGTCTAAGATGGCTTAATCACCTACATCCTAATTTAGAAAAGGTTCCAATTACTAGAGAAGAAGAACAGAAAATCGTTGAATTGCATGCCAAGATCGGACCCAAATGGTCTCTATGGTTAAGGAGGACTGTATATCCTATGGTCAGGATTGATAAAAACAAATATATCCTATGGTTAGGAATTGCTTATTTGGCAATCCTCGTCAAATTAATCTTTTTGGAATTGCTTATAATTCTAGCCTAA

Protein sequence

>Msa0032630

MSYGADTTRLKKGPWSLHEDAILKEYVRNYGEGNWDAVKEKTELFRCGKSCRLRWLNHLHPNLEKVPITREEEQKIVELHAKIGPKWSLWLRRTVYPMVRIDKNKYILWLGIAYLAILVKLIFLELLIILA*