AlfalfaGEDB | Msa0032640

M. sativa cultivar zhongmu-4 / Msa0032640

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF13921.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032640	ABD28344.1	62.873	369	71	13	3	318	15	370	5.85e-132	392

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032640	sp\|Q8W1W6\|MYB33_ARATH	53.913	115	43	1	3	107	35	149	5.94e-34	133

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0032640	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032640	MtrunA17_Chr1g0192411	62.873	369	71	13	3	318	15	370	5.38e-136	392
Msa0032640	MtrunA17_Chr1g0154031	50.000	132	50	2	3	124	79	204	9.64e-35	130
Msa0032640	MtrunA17_Chr8g0354661	53.982	113	42	1	3	105	40	152	4.38e-33	128
Msa0032640	MtrunA17_Chr3g0099131	53.571	112	42	1	3	104	44	155	1.67e-32	126
Msa0032640	MtrunA17_Chr3g0080861	49.558	113	47	1	3	105	40	152	1.43e-31	124
Msa0032640	MtrunA17_Chr2g0322521	52.174	115	45	1	3	107	32	146	5.53e-30	117
Msa0032640	MtrunA17_Chr5g0440781	39.227	181	87	6	3	171	15	184	1.53e-29	111
Msa0032640	MtrunA17_Chr5g0441141	39.227	181	87	6	3	171	15	184	5.33e-29	112
Msa0032640	MtrunA17_Chr5g0440761	39.227	181	87	6	3	171	15	184	5.41e-29	112
Msa0032640	MtrunA17_Chr3g0099141	60.759	79	31	0	3	81	43	121	6.87e-27	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032640	AT5G06100.1	52.991	117	45	1	3	109	35	151	2.27e-35	133
Msa0032640	AT5G06100.5	53.913	115	43	1	3	107	35	149	6.04e-35	133
Msa0032640	AT5G06100.2	53.913	115	43	1	3	107	35	149	6.04e-35	133
Msa0032640	AT5G06100.3	53.913	115	43	1	3	107	35	149	6.04e-35	133
Msa0032640	AT5G06100.4	53.913	115	43	1	3	107	35	149	6.04e-35	133
Msa0032640	AT2G32460.1	51.282	117	47	1	3	109	21	137	1.07e-34	132
Msa0032640	AT2G32460.2	51.282	117	47	1	3	109	9	125	1.26e-34	132
Msa0032640	AT4G26930.1	50.862	116	47	1	3	108	22	137	1.57e-34	130
Msa0032640	AT3G11440.2	53.097	113	43	1	3	105	44	156	8.56e-34	129
Msa0032640	AT3G11440.3	53.097	113	43	1	3	105	44	156	2.33e-33	129

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Find 47 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGACCATGGTCACTTG+AGG	0.594806	1_1:+59813743	Msa0032640:intron
AAATATGCTATGCAACAATG+TGG	0.683001	1_1:+59815414	Msa0032640:CDS
AAGATCTGATTCCGTGGCTT+TGG	0.389101	1_1:-59815590	None:intergenic
AATCATGAATTGAATGCCAA+TGG	0.537217	1_1:+59815229	Msa0032640:CDS
AATGGAGAAACTTGTCTGAT+TGG	0.452375	1_1:+59816295	Msa0032640:CDS
ACAAGGAAACGCACAAAACT+TGG	0.439340	1_1:+59815193	Msa0032640:CDS
ACTCAGACTACTTTCTCCAT+TGG	0.498643	1_1:-59815245	None:intergenic
ACTTACGTGATTGTTAATGT+TGG	0.581736	1_1:-59815466	None:intergenic
ACTTCCACTTCGATCACCTA+TGG	0.464348	1_1:+59816501	Msa0032640:CDS
ACTTCTGGAATATAAGAACA+AGG	0.663405	1_1:+59815176	Msa0032640:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAGATTGTTAACGAAGTT+CGG	-	chr1_1:59813572-59813591	None:intergenic	30.0%
AAGTAGAAGTGGAATAAATG+CGG	-	chr1_1:59813606-59813625	None:intergenic	30.0%
AAGCAAGAAAAATGTAACGA+TGG	+	chr1_1:59813702-59813721	Msa0032640:intron	30.0%
ATACATTGAAAAAAGGACCA+TGG	+	chr1_1:59813733-59813752	Msa0032640:intron	30.0%
AGAGTATACGTAAGTCAATA+TGG	+	chr1_1:59813780-59813799	Msa0032640:CDS	30.0%
TAATTAAGTACGTACGGAAT+AGG	-	chr1_1:59814140-59814159	None:intergenic	30.0%
ATTCCGTACGTACTTAATTA+TGG	+	chr1_1:59814140-59814159	Msa0032640:intron	30.0%
GAACCATAATTAAGTACGTA+CGG	-	chr1_1:59814146-59814165	None:intergenic	30.0%
GTACTTAATTATGGTTCAAG+AGG	+	chr1_1:59814149-59814168	Msa0032640:intron	30.0%
TAATCCAAAATAACAGTGTG+TGG	-	chr1_1:59814452-59814471	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	59813250	59816549	59813250	ID=Msa0032640;Name=Msa0032640
chr1_1	mRNA	59813250	59816549	59813250	ID=Msa0032640-mRNA-1;Parent=Msa0032640;Name=Msa0032640-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0\|0.4\|0.16\|0.83\|0.4\|0.33\|6\|0\|326
chr1_1	exon	59813250	59813256	59813250	ID=Msa0032640-mRNA-1:exon:18585;Parent=Msa0032640-mRNA-1
chr1_1	exon	59813748	59813989	59813748	ID=Msa0032640-mRNA-1:exon:18586;Parent=Msa0032640-mRNA-1
chr1_1	exon	59815175	59815340	59815175	ID=Msa0032640-mRNA-1:exon:18587;Parent=Msa0032640-mRNA-1
chr1_1	exon	59815350	59815482	59815350	ID=Msa0032640-mRNA-1:exon:18588;Parent=Msa0032640-mRNA-1
chr1_1	exon	59815570	59815660	59815570	ID=Msa0032640-mRNA-1:exon:18589;Parent=Msa0032640-mRNA-1
chr1_1	exon	59816208	59816549	59816208	ID=Msa0032640-mRNA-1:exon:18590;Parent=Msa0032640-mRNA-1
chr1_1	CDS	59813250	59813256	59813250	ID=Msa0032640-mRNA-1:cds;Parent=Msa0032640-mRNA-1
chr1_1	CDS	59813748	59813989	59813748	ID=Msa0032640-mRNA-1:cds;Parent=Msa0032640-mRNA-1
chr1_1	CDS	59815175	59815340	59815175	ID=Msa0032640-mRNA-1:cds;Parent=Msa0032640-mRNA-1
chr1_1	CDS	59815350	59815482	59815350	ID=Msa0032640-mRNA-1:cds;Parent=Msa0032640-mRNA-1
chr1_1	CDS	59815570	59815660	59815570	ID=Msa0032640-mRNA-1:cds;Parent=Msa0032640-mRNA-1
chr1_1	CDS	59816208	59816549	59816208	ID=Msa0032640-mRNA-1:cds;Parent=Msa0032640-mRNA-1

Gene Sequence

>Msa0032640

ATGTTAGGACCATGGTCACTTGAGGAAGATGAAAAATTAAGAGTATACGTAAGTCAATATGGAGAAGGCAACTGGGATGCAGTTAGAAAGAAAACCGAACTTTTTCGATGTGTTAAAAGTTGTCGTTTAAGATGGCTTAATCACCTAAATCCTAATTTAAAAAAGGGTCCAATTACTAAAGAAGAAGAACTTAAAATCGTTGAACTTCATGCCAAGATCGGACCCAAATGGTCTCTAATGGTTCAAGAGAACTTCTGGAATATAAGAACAAGGAAACGCACAAAACTTGGTTTGCCACTCTACAATCATGAATTGAATGCCAATGGAGAAAGTAGTCTGAGTACTTTGAGCAACCCGGAGCAAGCTGATGTCTCACATGAAATGAAATTCAAAAATGATATTTTCAGCAGAAAACATTCAAATGAGTTTGAACAACTTTCAAACTCATTGCATGTCTCAATTACACGGCCTCATTTTTCAAATATGCTATGCAACAATGTGGTCAATACTTCTATAAACAATAATGCTTCACCAACATTAACAATCACGGATGGAACTCCTTTTAGGACCAAAGCCACGGAATCAGATCTTCCACAATTTCAAGGGTGCAACTCCAAGTGCTATAAAATTGTTGAAGAGTTGCCTGCAAGAAGAGGAAATGAGATAGATAACTACCATAATATAAGAACAAAGAAACGTGCAAGAGCTGATGACGAATGGAATGACAATGGAGAAACTTGTCTGATTGGTTTGAGCAACCAACATCAAGCTGATGTCTCAAAATCAGACAAATCCGACATACATGGAGTGAACTTCAAAAAAGACATTTTTGGCGGAAAAGATCCAAAAATTTTGAATGAAATAAATGGAAGCAACTTTGAAGAACTTTCAAACTCATTTTATTCATATTCACTACTTCACAAGTATCCTTTACTTCCACTTCGATCACCTATGGAGATCATTAAAGCAAGACAAAAATGA

Protein sequence

>Msa0032640

MLGPWSLEEDEKLRVYVSQYGEGNWDAVRKKTELFRCVKSCRLRWLNHLNPNLKKGPITKEEELKIVELHAKIGPKWSLMVQENFWNIRTRKRTKLGLPLYNHELNANGESSLSTLSNPEQADVSHEMKFKNDIFSRKHSNEFEQLSNSLHVSITRPHFSNMLCNNVVNTSINNNASPTLTITDGTPFRTKATESDLPQFQGCNSKCYKIVEELPARRGNEIDNYHNIRTKKRARADDEWNDNGETCLIGLSNQHQADVSKSDKSDIHGVNFKKDIFGGKDPKILNEINGSNFEELSNSFYSYSLLHKYPLLPLRSPMEIIKARQK*