AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0036360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036360 MtrunA17_Chr1g0198801 98.824 340 1 2 1 339 1 338 0.0 689
Msa0036360 MtrunA17_Chr7g0255961 49.738 191 89 3 142 331 58 242 7.48e-53 174
Msa0036360 MtrunA17_Chr3g0113721 41.935 217 112 4 124 331 9 220 4.38e-49 164
Msa0036360 MtrunA17_Chr4g0041051 40.625 192 103 2 155 339 64 251 5.13e-31 117
Msa0036360 MtrunA17_Chr2g0325521 42.458 179 92 3 145 316 45 219 1.98e-30 115
Msa0036360 MtrunA17_Chr5g0412371 36.145 166 89 3 157 316 43 197 6.00e-30 113
Msa0036360 MtrunA17_Chr5g0427361 34.934 229 107 4 136 327 8 231 1.16e-26 106
Msa0036360 MtrunA17_Chr4g0045901 43.407 182 89 6 143 317 62 236 1.05e-24 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036360 AT1G68810.1 64.394 264 68 8 96 337 99 358 1.21e-93 283
Msa0036360 AT3G25710.1 50.000 320 97 8 72 333 22 336 7.99e-80 247
Msa0036360 AT3G56770.1 47.059 170 82 4 151 317 37 201 6.30e-43 148
Msa0036360 AT2G41130.1 46.629 178 84 5 143 317 55 224 2.44e-38 137
Msa0036360 AT2G40200.1 42.105 190 89 8 130 311 38 214 1.45e-33 124
Msa0036360 AT4G38070.1 37.324 142 77 3 153 294 87 216 3.91e-25 102
Msa0036360 AT1G01260.1 31.111 180 99 8 38 207 314 478 1.25e-11 66.2
Msa0036360 AT1G01260.2 31.111 180 99 8 38 207 314 478 1.25e-11 66.2
Msa0036360 AT1G01260.3 31.111 180 99 8 38 207 314 478 1.25e-11 66.2
Msa0036360 AT5G57150.1 25.137 183 99 5 166 319 58 231 9.04e-11 62.0

Find 85 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAGAATTGATTAAATTA+TGG 0.270913 1_1:-64859271 Msa0036360:three_prime_UTR
ATTATTATTATATTAGTATA+TGG 0.273119 1_1:-64859218 Msa0036360:three_prime_UTR
ATGCAAGTTTGTTCAATAGA+AGG 0.283417 1_1:-64861085 Msa0036360:CDS
GATGAGTGCTCAAGAAATTA+TGG 0.286315 1_1:-64860937 Msa0036360:CDS
TCAAGACATTGAAAGCATTA+AGG 0.296728 1_1:-64859499 Msa0036360:CDS
TGTTCTAATTGATTCTATAA+TGG 0.302929 1_1:+64861810 None:intergenic
TTCAATGTCTTGATTAGTTC+AGG 0.303436 1_1:+64859509 None:intergenic
ATGCAGGTTCTGAGCATCTT+AGG 0.326044 1_1:-64861031 Msa0036360:CDS
TATACTAATATAATAATAAT+AGG 0.326223 1_1:+64859221 None:intergenic
GCAGATGATGATGAAGATTA+TGG 0.331671 1_1:-64859596 Msa0036360:CDS
GTTGGAGATGAATCTGCTTC+TGG 0.334849 1_1:-64859338 Msa0036360:CDS
AATAGTGATCATCATATCTT+TGG 0.353704 1_1:-64861266 Msa0036360:CDS
TGATCTTCTTGTATCATACT+TGG 0.359086 1_1:+64861377 None:intergenic
GTAAGATGTTGTTATGTATA+TGG 0.364483 1_1:-64859090 Msa0036360:three_prime_UTR
GGATCAGGTTCAGGCCCCTT+TGG 0.366768 1_1:-64860984 Msa0036360:CDS
ACAATGAGGTAATGATAATT+TGG 0.372247 1_1:-64859045 Msa0036360:three_prime_UTR
GACCATGTTTGTTGCAAGAT+TGG 0.409233 1_1:+64861188 None:intergenic
GTTTGTTTCTCCTGTGGTGG+TGG 0.415527 1_1:+64861861 None:intergenic
GATGAAGATTATGGATCAAA+TGG 0.416803 1_1:-64859587 Msa0036360:CDS
TTCAGCTTCACTATGACTCT+TGG 0.417321 1_1:+64860893 None:intergenic
TTATATTAGTATATGGTACT+TGG 0.421823 1_1:-64859211 Msa0036360:three_prime_UTR
TCATCACATTCAGTTGGAAC+TGG 0.424698 1_1:+64859635 None:intergenic
CCTCACTTTGCTGTGATGAT+AGG 0.438078 1_1:-64859544 Msa0036360:CDS
ATACTTACTTTGGTTGTGCT+TGG 0.449724 1_1:+64860816 None:intergenic
CTATTGAACAAACTTGCATA+AGG 0.450006 1_1:+64861089 None:intergenic
GAGAGATAAATCTAAAGCCT+TGG 0.462647 1_1:-64861900 Msa0036360:five_prime_UTR
AGAGAGCAACAAAGCACAAA+GGG 0.469426 1_1:-64862041 Msa0036360:five_prime_UTR
GCCTCATCATCTGTTGTTGA+AGG 0.470688 1_1:+64861294 None:intergenic
CTTTGGCCGCCCACCACCAC+AGG 0.474997 1_1:-64861871 Msa0036360:five_prime_UTR
TGCAAGTTTGTTCAATAGAA+GGG 0.484018 1_1:-64861084 Msa0036360:CDS
CTTCAACATGGATCAGGTTC+AGG 0.485258 1_1:-64860993 Msa0036360:CDS
TCAGGTTCAGGCCCCTTTGG+TGG 0.487292 1_1:-64860981 Msa0036360:CDS
TTCTTTGTTTGTTTCTCCTG+TGG 0.494108 1_1:+64861855 None:intergenic
CTAATTGATTCTATAATGGT+GGG 0.495009 1_1:+64861814 None:intergenic
GTTAATTCATCACATTCAGT+TGG 0.495856 1_1:+64859629 None:intergenic
TTCCCTACAGCAGGAGGAGG+GGG 0.504915 1_1:-64861227 Msa0036360:CDS
TCTAATTGATTCTATAATGG+TGG 0.508231 1_1:+64861813 None:intergenic
ATGAGGTAATGATAATTTGG+TGG 0.510319 1_1:-64859042 Msa0036360:three_prime_UTR
GCAACAAAGCACAAAGGGAA+AGG 0.511848 1_1:-64862036 Msa0036360:five_prime_UTR
TCAAATCTCTTTCACTTGGT+AGG 0.518755 1_1:+64861954 None:intergenic
TCACTACGCACAAATGTTTG+TGG 0.519274 1_1:-64861986 Msa0036360:five_prime_UTR
TTTGTTTGTTTCTCCTGTGG+TGG 0.520687 1_1:+64861858 None:intergenic
AGTGATCATCATATCTTTGG+TGG 0.524209 1_1:-64861263 Msa0036360:CDS
TCACCTTGAAAACCACCAAA+GGG 0.525646 1_1:+64860969 None:intergenic
ATGTTCCCTACAGCAGGAGG+AGG 0.528325 1_1:-64861230 Msa0036360:CDS
AGGTCTATGTTCCCTACAGC+AGG 0.528893 1_1:-64861236 Msa0036360:CDS
GCTGATATAACAACACTTGG+AGG 0.532615 1_1:-64859461 Msa0036360:CDS
CACCAATCTTGCAACAAACA+TGG 0.533876 1_1:-64861190 Msa0036360:CDS
TTTGCATATGATCATCATGC+AGG 0.543634 1_1:-64861047 Msa0036360:CDS
TCAAGAAATTATGGAGGCTA+AGG 0.546278 1_1:-64860928 Msa0036360:CDS
AATTGATTCTATAATGGTGG+GGG 0.552128 1_1:+64861816 None:intergenic
GATACACTTCAACATGGATC+AGG 0.552437 1_1:-64860999 Msa0036360:CDS
TAATTGATTCTATAATGGTG+GGG 0.553540 1_1:+64861815 None:intergenic
TTTGTTTCTCCTGTGGTGGT+GGG 0.561793 1_1:+64861862 None:intergenic
ATCATATCTTTGGTGGAGGA+AGG 0.562826 1_1:-64861256 Msa0036360:CDS
TGTTCCCTACAGCAGGAGGA+GGG 0.570414 1_1:-64861229 Msa0036360:CDS
TCATATGCAAACTGAAGAGA+AGG 0.572922 1_1:+64861059 None:intergenic
AGAAGCACTCAAAGCTGTGA+TGG 0.573378 1_1:-64859369 Msa0036360:CDS
GTTTCTCCTGTGGTGGTGGG+CGG 0.576111 1_1:+64861865 None:intergenic
AGCTGCGTAGCTTAGCAAGA+TGG 0.576612 1_1:+64860844 None:intergenic
TCCTTCAACAACAGATGATG+AGG 0.577140 1_1:-64861295 Msa0036360:CDS
GATCATCATATCTTTGGTGG+AGG 0.579767 1_1:-64861260 Msa0036360:CDS
TTCACCTTGAAAACCACCAA+AGG 0.583876 1_1:+64860968 None:intergenic
GTTCCCTACAGCAGGAGGAG+GGG 0.584625 1_1:-64861228 Msa0036360:CDS
AATGTCAAATCTCTTTCACT+TGG 0.587124 1_1:+64861950 None:intergenic
AAAGGGTCATGAGGGTTGAA+AGG 0.588818 1_1:+64861137 None:intergenic
TGTTGTTGAGGAATAACGAA+AGG 0.589817 1_1:+64861119 None:intergenic
TAAGGTGAAGCTTGTTGTTG+AGG 0.593376 1_1:+64861107 None:intergenic
ATTAGAACAATATCTATCAA+AGG 0.593991 1_1:-64861796 Msa0036360:intron
AAGAGAGCAACAAAGCACAA+AGG 0.595215 1_1:-64862042 Msa0036360:five_prime_UTR
GTTGTTGAGGAATAACGAAA+GGG 0.597793 1_1:+64861120 None:intergenic
AGAGTCATAGTGAAGCTGAA+AGG 0.601072 1_1:-64860890 Msa0036360:CDS
AAAGCTGATATAACAACACT+TGG 0.602897 1_1:-64859464 Msa0036360:CDS
CTGGAAGTGTCAAGAGACAA+AGG 0.603991 1_1:-64859319 Msa0036360:CDS
GCTGTGATGGAGAAGAGTGT+TGG 0.619796 1_1:-64859356 Msa0036360:CDS
GAATAACGAAAGGGTCATGA+GGG 0.626099 1_1:+64861129 None:intergenic
CCTATCATCACAGCAAAGTG+AGG 0.634985 1_1:+64859544 None:intergenic
AGTATGATACAAGAAGATCA+AGG 0.636259 1_1:-64861374 Msa0036360:CDS
GGAATAACGAAAGGGTCATG+AGG 0.647080 1_1:+64861128 None:intergenic
TCTATGTTCCCTACAGCAGG+AGG 0.647545 1_1:-64861233 Msa0036360:CDS
GGCGGCCAAAGAAAAGTCCA+AGG 0.663085 1_1:+64861883 None:intergenic
CACCTTGAAAACCACCAAAG+GGG 0.675283 1_1:+64860970 None:intergenic
ATATCAGATACACTTCAACA+TGG 0.699194 1_1:-64861005 Msa0036360:CDS
GAGTGCTCAAGAAATTATGG+AGG 0.703562 1_1:-64860934 Msa0036360:CDS
AACATGATGATGAGACAATG+AGG 0.726295 1_1:-64859059 Msa0036360:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATATTAGTACTATAT+TGG + chr1_1:64860436-64860455 None:intergenic 10.0%
!! AAATTTATAGTTAATTAAAG+TGG + chr1_1:64861260-64861279 None:intergenic 10.0%
!! AATTAGAATTGATTAAATTA+TGG - chr1_1:64861810-64861829 Msa0036360:five_prime_UTR 10.0%
!!! TTTTTATCTTTTTTTTTTTG+GGG - chr1_1:64861925-64861944 Msa0036360:five_prime_UTR 10.0%
!! ATATATACATATGAATATGA+TGG + chr1_1:64859639-64859658 None:intergenic 15.0%
!! ATATGTATATATATAAACCT+TGG - chr1_1:64859647-64859666 Msa0036360:CDS 15.0%
!! TTACTAATATATACTTACTT+TGG + chr1_1:64860278-64860297 None:intergenic 15.0%
!!! AGATATTTTGAATTTACAAA+AGG + chr1_1:64860541-64860560 None:intergenic 15.0%
!!! TTGTTCTCTTTTTTTTTTTT+TGG - chr1_1:64860686-64860705 Msa0036360:intron 15.0%
!!! TTTTATCTTTTTTTTTTTGG+GGG - chr1_1:64861926-64861945 Msa0036360:five_prime_UTR 15.0%
!!! TGTTCTAATTGATTCTATAA+TGG + chr1_1:64859274-64859293 None:intergenic 20.0%
!! ATTAGAACAATATCTATCAA+AGG - chr1_1:64859285-64859304 Msa0036360:exon 20.0%
!! ACAAGGAATAAAAATGAAAA+GGG + chr1_1:64859381-64859400 None:intergenic 20.0%
!!! GATTTTGATTTTGTTGTTTA+TGG + chr1_1:64860576-64860595 None:intergenic 20.0%
!!! ATATAACCTTTTAGAAAATG+AGG - chr1_1:64860598-64860617 Msa0036360:intron 20.0%
!!! TGTAACTATATGTTTTGTTT+TGG - chr1_1:64860739-64860758 Msa0036360:intron 20.0%
!!! GTAACTATATGTTTTGTTTT+GGG - chr1_1:64860740-64860759 Msa0036360:intron 20.0%
!!! AATAGTGGATATATTTTGAA+TGG + chr1_1:64861047-64861066 None:intergenic 20.0%
!! TATGATATAAGAATGTGAAA+TGG + chr1_1:64861071-64861090 None:intergenic 20.0%
!! TGAAAATATTTACAATCTCA+TGG + chr1_1:64861145-64861164 None:intergenic 20.0%
!! TTATATTAGTATATGGTACT+TGG - chr1_1:64861870-64861889 Msa0036360:five_prime_UTR 20.0%
!!! TTTATCTTTTTTTTTTTGGG+GGG - chr1_1:64861927-64861946 Msa0036360:five_prime_UTR 20.0%
!!! TCTTTTCTTTTCTTTTTTCA+TGG - chr1_1:64861962-64861981 Msa0036360:five_prime_UTR 20.0%
!! TAATTGATTCTATAATGGTG+GGG + chr1_1:64859269-64859288 None:intergenic 25.0%
!! CTAATTGATTCTATAATGGT+GGG + chr1_1:64859270-64859289 None:intergenic 25.0%
!! TCTAATTGATTCTATAATGG+TGG + chr1_1:64859271-64859290 None:intergenic 25.0%
!!! AAATGAAAAGGGTATTTGAA+AGG + chr1_1:64859370-64859389 None:intergenic 25.0%
! CACAAGGAATAAAAATGAAA+AGG + chr1_1:64859382-64859401 None:intergenic 25.0%
! ACAAAATAAAACCTAGCTAT+AGG - chr1_1:64859582-64859601 Msa0036360:CDS 25.0%
! TATATATAAACCTTGGAGTT+TGG - chr1_1:64859654-64859673 Msa0036360:CDS 25.0%
! AATAGTGATCATCATATCTT+TGG - chr1_1:64859815-64859834 Msa0036360:intron 25.0%
!! GGTTTGCATTTTAAATTTCT+TGG + chr1_1:64860392-64860411 None:intergenic 25.0%
! GGCATATATTGAATTCAAAA+AGG + chr1_1:64860520-64860539 None:intergenic 25.0%
! TGAAAATATATTGTCTTTGC+CGG - chr1_1:64860904-64860923 Msa0036360:CDS 25.0%
! TGGAGAATGAAAAGAAATAA+AGG - chr1_1:64860954-64860973 Msa0036360:CDS 25.0%
! ATCTGATATATACATAAGGT+TGG + chr1_1:64861108-64861127 None:intergenic 25.0%
! ATGGATCTGATATATACATA+AGG + chr1_1:64861112-64861131 None:intergenic 25.0%
! ATATTTACAATCTCATGGTA+GGG + chr1_1:64861140-64861159 None:intergenic 25.0%
! AATATTTACAATCTCATGGT+AGG + chr1_1:64861141-64861160 None:intergenic 25.0%
!!! AAGAATGTTTTGTTCATTAC+TGG - chr1_1:64861650-64861669 Msa0036360:intron 25.0%
! GTAAGATGTTGTTATGTATA+TGG - chr1_1:64861991-64862010 Msa0036360:five_prime_UTR 25.0%
! ACAATGAGGTAATGATAATT+TGG - chr1_1:64862036-64862055 Msa0036360:five_prime_UTR 25.0%
!!! GTTTTATTTTTACATGTGTG+TGG - chr1_1:64862061-64862080 Msa0036360:five_prime_UTR 25.0%
AATGTCAAATCTCTTTCACT+TGG + chr1_1:64859134-64859153 None:intergenic 30.0%
! AATTGATTCTATAATGGTGG+GGG + chr1_1:64859268-64859287 None:intergenic 30.0%
GGTATTTGAAAGGACAAAAT+GGG + chr1_1:64859360-64859379 None:intergenic 30.0%
! TGTTTTATATCCAAACTCCA+AGG + chr1_1:64859667-64859686 None:intergenic 30.0%
TTGGAGTTTGGATATAAAAC+AGG - chr1_1:64859666-64859685 Msa0036360:CDS 30.0%
TGATCTTCTTGTATCATACT+TGG + chr1_1:64859707-64859726 None:intergenic 30.0%
AGTATGATACAAGAAGATCA+AGG - chr1_1:64859707-64859726 Msa0036360:CDS 30.0%
CTATTGAACAAACTTGCATA+AGG + chr1_1:64859995-64860014 None:intergenic 30.0%
! ATGCAAGTTTGTTCAATAGA+AGG - chr1_1:64859996-64860015 Msa0036360:intron 30.0%
! TGCAAGTTTGTTCAATAGAA+GGG - chr1_1:64859997-64860016 Msa0036360:intron 30.0%
ATATCAGATACACTTCAACA+TGG - chr1_1:64860076-64860095 Msa0036360:intron 30.0%
!! TTGAGTTGAGATCTTTGATT+AGG + chr1_1:64860413-64860432 None:intergenic 30.0%
! TTTGCACCTCATTTTCTAAA+AGG + chr1_1:64860607-64860626 None:intergenic 30.0%
! GCTTTTGTTAACATCCTTTT+TGG - chr1_1:64860646-64860665 Msa0036360:intron 30.0%
GAATTAGTGTTAATGTCTCT+TGG - chr1_1:64860835-64860854 Msa0036360:CDS 30.0%
TGACTGATCAACATATTATC+CGG + chr1_1:64860926-64860945 None:intergenic 30.0%
!! AGTGGATATATTTTGAATGG+TGG + chr1_1:64861044-64861063 None:intergenic 30.0%
AGAATGTGAAATGGAAATAG+TGG + chr1_1:64861062-64861081 None:intergenic 30.0%
! GATATATACATAAGGTTGGT+AGG + chr1_1:64861104-64861123 None:intergenic 30.0%
GTTAATTCATCACATTCAGT+TGG + chr1_1:64861455-64861474 None:intergenic 30.0%
GATGAAGATTATGGATCAAA+TGG - chr1_1:64861494-64861513 Msa0036360:intron 30.0%
! TTCAATGTCTTGATTAGTTC+AGG + chr1_1:64861575-64861594 None:intergenic 30.0%
!! TCAAGACATTGAAAGCATTA+AGG - chr1_1:64861582-64861601 Msa0036360:intron 30.0%
AAAGCTGATATAACAACACT+TGG - chr1_1:64861617-64861636 Msa0036360:intron 30.0%
ATGAGGTAATGATAATTTGG+TGG - chr1_1:64862039-64862058 Msa0036360:five_prime_UTR 30.0%
TCAAATCTCTTTCACTTGGT+AGG + chr1_1:64859130-64859149 None:intergenic 35.0%
! GAGAGATAAATCTAAAGCCT+TGG - chr1_1:64859181-64859200 Msa0036360:three_prime_UTR 35.0%
! TAAAGCCTTGGACTTTTCTT+TGG - chr1_1:64859193-64859212 Msa0036360:three_prime_UTR 35.0%
! TTCTTTGTTTGTTTCTCCTG+TGG + chr1_1:64859229-64859248 None:intergenic 35.0%
GGGTATTTGAAAGGACAAAA+TGG + chr1_1:64859361-64859380 None:intergenic 35.0%
TATAGGCACTTAATTGCACA+AGG + chr1_1:64859398-64859417 None:intergenic 35.0%
AAATGTCAGAACCTATAGCT+AGG + chr1_1:64859596-64859615 None:intergenic 35.0%
AGTGATCATCATATCTTTGG+TGG - chr1_1:64859818-64859837 Msa0036360:intron 35.0%
GTTGTTGAGGAATAACGAAA+GGG + chr1_1:64859964-64859983 None:intergenic 35.0%
TGTTGTTGAGGAATAACGAA+AGG + chr1_1:64859965-64859984 None:intergenic 35.0%
TCATATGCAAACTGAAGAGA+AGG + chr1_1:64860025-64860044 None:intergenic 35.0%
TTTGCATATGATCATCATGC+AGG - chr1_1:64860034-64860053 Msa0036360:intron 35.0%
GATGAGTGCTCAAGAAATTA+TGG - chr1_1:64860144-64860163 Msa0036360:intron 35.0%
TCAAGAAATTATGGAGGCTA+AGG - chr1_1:64860153-64860172 Msa0036360:intron 35.0%
ATACTTACTTTGGTTGTGCT+TGG + chr1_1:64860268-64860287 None:intergenic 35.0%
!!! GCAAGAGTGTTTTTTCTAAG+TGG - chr1_1:64860494-64860513 Msa0036360:intron 35.0%
!!! AACATCCTTTTTGGCTTGTT+TGG - chr1_1:64860655-64860674 Msa0036360:intron 35.0%
GTATACCAAACAAGCCAAAA+AGG + chr1_1:64860663-64860682 None:intergenic 35.0%
TATAATGTGTGGTAGTTCCA+AGG - chr1_1:64860771-64860790 Msa0036360:intron 35.0%
TGTCTCTTGGTATGATAAAG+AGG - chr1_1:64860848-64860867 Msa0036360:CDS 35.0%
!! TTTTCAACTGCATGCTGTTT+TGG + chr1_1:64860876-64860895 None:intergenic 35.0%
GTTGATCAGTCAAACTCAAT+TGG - chr1_1:64860934-64860953 Msa0036360:CDS 35.0%
GTTATGTTTGTGACTGACAA+AGG + chr1_1:64860998-64861017 None:intergenic 35.0%
! GCAGATGATGATGAAGATTA+TGG - chr1_1:64861485-64861504 Msa0036360:intron 35.0%
!!! ATCTTTTTTTTTTTGGGGGG+TGG - chr1_1:64861930-64861949 Msa0036360:five_prime_UTR 35.0%
! AACATGATGATGAGACAATG+AGG - chr1_1:64862022-64862041 Msa0036360:five_prime_UTR 35.0%
!! GTGCTTTGTTGCTCTCTTTT+TGG + chr1_1:64859037-64859056 None:intergenic 40.0%
AAGAGAGCAACAAAGCACAA+AGG - chr1_1:64859039-64859058 Msa0036360:three_prime_UTR 40.0%
AGAGAGCAACAAAGCACAAA+GGG - chr1_1:64859040-64859059 Msa0036360:three_prime_UTR 40.0%
TCACTACGCACAAATGTTTG+TGG - chr1_1:64859095-64859114 Msa0036360:three_prime_UTR 40.0%
! TTTGTTTGTTTCTCCTGTGG+TGG + chr1_1:64859226-64859245 None:intergenic 40.0%
TCCTTCAACAACAGATGATG+AGG - chr1_1:64859786-64859805 Msa0036360:intron 40.0%
GATCATCATATCTTTGGTGG+AGG - chr1_1:64859821-64859840 Msa0036360:intron 40.0%
ATCATATCTTTGGTGGAGGA+AGG - chr1_1:64859825-64859844 Msa0036360:intron 40.0%
CACCAATCTTGCAACAAACA+TGG - chr1_1:64859891-64859910 Msa0036360:intron 40.0%
! GACCATGTTTGTTGCAAGAT+TGG + chr1_1:64859896-64859915 None:intergenic 40.0%
GAATAACGAAAGGGTCATGA+GGG + chr1_1:64859955-64859974 None:intergenic 40.0%
!! TAAGGTGAAGCTTGTTGTTG+AGG + chr1_1:64859977-64859996 None:intergenic 40.0%
GATACACTTCAACATGGATC+AGG - chr1_1:64860082-64860101 Msa0036360:intron 40.0%
TCACCTTGAAAACCACCAAA+GGG + chr1_1:64860115-64860134 None:intergenic 40.0%
TTCACCTTGAAAACCACCAA+AGG + chr1_1:64860116-64860135 None:intergenic 40.0%
GAGTGCTCAAGAAATTATGG+AGG - chr1_1:64860147-64860166 Msa0036360:intron 40.0%
TTCAGCTTCACTATGACTCT+TGG + chr1_1:64860191-64860210 None:intergenic 40.0%
AGAGTCATAGTGAAGCTGAA+AGG - chr1_1:64860191-64860210 Msa0036360:intron 40.0%
CTAGCTGGCTAAAGAAAAAG+TGG - chr1_1:64860800-64860819 Msa0036360:intron 40.0%
TCATCACATTCAGTTGGAAC+TGG + chr1_1:64861449-64861468 None:intergenic 40.0%
GCTGATATAACAACACTTGG+AGG - chr1_1:64861620-64861639 Msa0036360:intron 40.0%
GCAACAAAGCACAAAGGGAA+AGG - chr1_1:64859045-64859064 Msa0036360:three_prime_UTR 45.0%
! TTTGTTTCTCCTGTGGTGGT+GGG + chr1_1:64859222-64859241 None:intergenic 45.0%
GCCTCATCATCTGTTGTTGA+AGG + chr1_1:64859790-64859809 None:intergenic 45.0%
AAAGGGTCATGAGGGTTGAA+AGG + chr1_1:64859947-64859966 None:intergenic 45.0%
GGAATAACGAAAGGGTCATG+AGG + chr1_1:64859956-64859975 None:intergenic 45.0%
!! ATGCAGGTTCTGAGCATCTT+AGG - chr1_1:64860050-64860069 Msa0036360:intron 45.0%
CTTCAACATGGATCAGGTTC+AGG - chr1_1:64860088-64860107 Msa0036360:intron 45.0%
CACCTTGAAAACCACCAAAG+GGG + chr1_1:64860114-64860133 None:intergenic 45.0%
!! GGTGGTTTTCAAGGTGAAGT+TGG - chr1_1:64860118-64860137 Msa0036360:intron 45.0%
!! GGGAGTGTCTCTATAATGTG+TGG - chr1_1:64860760-64860779 Msa0036360:intron 45.0%
ATCTCATGGTAGGGCTTGAA+TGG + chr1_1:64861131-64861150 None:intergenic 45.0%
CCTATCATCACAGCAAAGTG+AGG + chr1_1:64861540-64861559 None:intergenic 45.0%
! CCTCACTTTGCTGTGATGAT+AGG - chr1_1:64861537-64861556 Msa0036360:intron 45.0%
!! AGAAGCACTCAAAGCTGTGA+TGG - chr1_1:64861712-64861731 Msa0036360:intron 45.0%
GTTGGAGATGAATCTGCTTC+TGG - chr1_1:64861743-64861762 Msa0036360:intron 45.0%
!! CTGGAAGTGTCAAGAGACAA+AGG - chr1_1:64861762-64861781 Msa0036360:intron 45.0%
!! AAATAAAAAAAAATCTATAT+AGG + chr1_1:64859415-64859434 None:intergenic 5.0%
!!! TATAAATTTTATCTAATTAA+AGG - chr1_1:64861837-64861856 Msa0036360:five_prime_UTR 5.0%
!! TATACTAATATAATAATAAT+AGG + chr1_1:64861863-64861882 None:intergenic 5.0%
!! ATTATTATTATATTAGTATA+TGG - chr1_1:64861863-64861882 Msa0036360:five_prime_UTR 5.0%
!!! GTTATTATATATTTTTTATT+TGG - chr1_1:64861892-64861911 Msa0036360:five_prime_UTR 5.0%
!!! AATTTTTATCTTTTTTTTTT+TGG - chr1_1:64861923-64861942 Msa0036360:five_prime_UTR 5.0%
!!! ATTTTTATCTTTTTTTTTTT+GGG - chr1_1:64861924-64861943 Msa0036360:five_prime_UTR 5.0%
! GTTTGTTTCTCCTGTGGTGG+TGG + chr1_1:64859223-64859242 None:intergenic 50.0%
AGGTCTATGTTCCCTACAGC+AGG - chr1_1:64859845-64859864 Msa0036360:intron 50.0%
TCTATGTTCCCTACAGCAGG+AGG - chr1_1:64859848-64859867 Msa0036360:intron 50.0%
AGCTGCGTAGCTTAGCAAGA+TGG + chr1_1:64860240-64860259 None:intergenic 50.0%
TGAGCTGAGCTGAGATCTCA+TGG + chr1_1:64861171-64861190 None:intergenic 50.0%
! GCTGTGATGGAGAAGAGTGT+TGG - chr1_1:64861725-64861744 Msa0036360:intron 50.0%
GGCGGCCAAAGAAAAGTCCA+AGG + chr1_1:64859201-64859220 None:intergenic 55.0%
ATGTTCCCTACAGCAGGAGG+AGG - chr1_1:64859851-64859870 Msa0036360:intron 55.0%
TGTTCCCTACAGCAGGAGGA+GGG - chr1_1:64859852-64859871 Msa0036360:intron 55.0%
!! GGCCCCTTTGGTGGTTTTCA+AGG - chr1_1:64860109-64860128 Msa0036360:intron 55.0%
GTTCCAAGGAAGCAGCTAGC+TGG - chr1_1:64860785-64860804 Msa0036360:intron 55.0%
TAGCCAGCTAGCTGCTTCCT+TGG + chr1_1:64860791-64860810 None:intergenic 55.0%
!! GTTTCTCCTGTGGTGGTGGG+CGG + chr1_1:64859219-64859238 None:intergenic 60.0%
GTTCCCTACAGCAGGAGGAG+GGG - chr1_1:64859853-64859872 Msa0036360:intron 60.0%
TTCCCTACAGCAGGAGGAGG+GGG - chr1_1:64859854-64859873 Msa0036360:intron 60.0%
GGATCAGGTTCAGGCCCCTT+TGG - chr1_1:64860097-64860116 Msa0036360:intron 60.0%
TCAGGTTCAGGCCCCTTTGG+TGG - chr1_1:64860100-64860119 Msa0036360:intron 60.0%
CACCCCCTCCTCCTGCTGTA+GGG + chr1_1:64859859-64859878 None:intergenic 65.0%
TCACCCCCTCCTCCTGCTGT+AGG + chr1_1:64859860-64859879 None:intergenic 65.0%
CTTTGGCCGCCCACCACCAC+AGG - chr1_1:64859210-64859229 Msa0036360:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 64859013 64862090 64859013 ID=Msa0036360;Name=Msa0036360
chr1_1 mRNA 64859013 64862090 64859013 ID=Msa0036360-mRNA-1;Parent=Msa0036360;Name=Msa0036360-mRNA-1;_AED=0.00;_eAED=0.00;_QI=316|1|1|1|0.5|0.33|3|279|339
chr1_1 exon 64859013 64859741 64859013 ID=Msa0036360-mRNA-1:exon:20845;Parent=Msa0036360-mRNA-1
chr1_1 exon 64860824 64861415 64860824 ID=Msa0036360-mRNA-1:exon:20844;Parent=Msa0036360-mRNA-1
chr1_1 exon 64861797 64862090 64861797 ID=Msa0036360-mRNA-1:exon:20843;Parent=Msa0036360-mRNA-1
chr1_1 five_prime_UTR 64861797 64862090 64861797 ID=Msa0036360-mRNA-1:five_prime_utr;Parent=Msa0036360-mRNA-1
chr1_1 five_prime_UTR 64861394 64861415 64861394 ID=Msa0036360-mRNA-1:five_prime_utr;Parent=Msa0036360-mRNA-1
chr1_1 CDS 64860824 64861393 64860824 ID=Msa0036360-mRNA-1:cds;Parent=Msa0036360-mRNA-1
chr1_1 CDS 64859292 64859741 64859292 ID=Msa0036360-mRNA-1:cds;Parent=Msa0036360-mRNA-1
chr1_1 three_prime_UTR 64859013 64859291 64859013 ID=Msa0036360-mRNA-1:three_prime_utr;Parent=Msa0036360-mRNA-1
Gene Sequence

>Msa0036360

ATGATACAAGAAGATCAAGGACAGTGTTCATCTCAAACAATCAATAACTTTGAAACTTATCAACAAGAGCAGTTTCTCCTTCAACAACAGATGATGAGGCAACAAAATAGTGATCATCATATCTTTGGTGGAGGAAGGTCTATGTTCCCTACAGCAGGAGGAGGGGGTGATCATCAAGTTTCACCAATCTTGCAACAAACATGGTCTATGCAACAACTTCATCATCATCATCATCCTTTCAACCCTCATGACCCTTTCGTTATTCCTCAACAACAAGCTTCACCTTATGCAAGTTTGTTCAATAGAAGGGTTCCTTCTCTTCAGTTTGCATATGATCATCATGCAGGTTCTGAGCATCTTAGGATCATATCAGATACACTTCAACATGGATCAGGTTCAGGCCCCTTTGGTGGTTTTCAAGGTGAAGTTGGAAAGATGAGTGCTCAAGAAATTATGGAGGCTAAGGCTCTTGCTGCTTCCAAGAGTCATAGTGAAGCTGAAAGGAGACGCAGAGAGAGAATCAATAACCATCTTGCTAAGCTACGCAGCTTGTTGCCAAGCACAACCAAAACAGACAAAGCATCATTGCTAGCAGAAGTGATACAACATGTAAAAGAATTGAAGCGTCAAACATCATTGATAGCAGAAACAAGTCCAGTTCCAACTGAATGTGATGAATTAACAGTAGATGCTGCAGATGATGATGAAGATTATGGATCAAATGGAAACAAGTTTATCATAAAAGCCTCACTTTGCTGTGATGATAGGTCAGATCTTTTGCCTGAACTAATCAAGACATTGAAAGCATTAAGGTTAAGAACACTCAAAGCTGATATAACAACACTTGGAGGACGTGTTAAGAATGTTTTGTTCATTACTGGTGAAGAAGATGATCATGAATATTGCATTAGTTCAATACAAGAAGCACTCAAAGCTGTGATGGAGAAGAGTGTTGGAGATGAATCTGCTTCTGGAAGTGTCAAGAGACAAAGGACTAATATAATATCAATATCTAATTAA
Protein sequence

>Msa0036360

MIQEDQGQCSSQTINNFETYQQEQFLLQQQMMRQQNSDHHIFGGGRSMFPTAGGGGDHQVSPILQQTWSMQQLHHHHHPFNPHDPFVIPQQQASPYASLFNRRVPSLQFAYDHHAGSEHLRIISDTLQHGSGSGPFGGFQGEVGKMSAQEIMEAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPVPTECDELTVDAADDDEDYGSNGNKFIIKASLCCDDRSDLLPELIKTLKALRLRTLKADITTLGGRVKNVLFITGEEDDHEYCISSIQEALKAVMEKSVGDESASGSVKRQRTNIISISN*