AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0039300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0039300 MtrunA17_Chr1g0203441 90.909 154 8 2 39 190 92 241 1.53e-82 246
Msa0039300 MtrunA17_Chr6g0470971 57.692 156 43 3 39 171 93 248 7.24e-50 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0039300 AT5G52260.1 41.509 106 46 4 39 128 91 196 2.59e-11 61.6

Find 48 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAACCAACCTGCCTTAAT+TGG 0.183204 1_1:-68850709 None:intergenic
AACAACCATGACCATGTTTA+AGG 0.206159 1_1:-68850675 None:intergenic
TACAAATTCAGTTGAATCTT+TGG 0.229080 1_1:+68852535 Msa0039300:CDS
ATCTCATTGTCTGTCCTTCC+TGG 0.240508 1_1:-68852266 None:intergenic
CTGAGTTTGTGATCTTCTTC+AGG 0.242651 1_1:-68850643 None:intergenic
GCAAATCAAATTGTGGGAAA+TGG 0.281472 1_1:+68852691 Msa0039300:CDS
TTGGAGCTCTGTCCCAATTA+AGG 0.299321 1_1:+68850696 Msa0039300:CDS
CATTCAGCAAACAATAGTTT+TGG 0.334613 1_1:-68852479 None:intergenic
TGATTCCATTTCCATAGCTT+TGG 0.339299 1_1:-68852327 None:intergenic
GGTGCTTTGATGTGTCTCTT+TGG 0.342200 1_1:-68852453 None:intergenic
CAAATCAAATTGTGGGAAAT+GGG 0.348116 1_1:+68852692 Msa0039300:CDS
TTTATTAGGCAAAACAAAAT+GGG 0.375550 1_1:+68850570 Msa0039300:five_prime_UTR
AACTTTCCTCTCTTTAATCC+TGG 0.382456 1_1:-68851146 None:intergenic
CAAAACTATTGTTTGCTGAA+TGG 0.398678 1_1:+68852480 Msa0039300:CDS
GGGTTTGTCCATTGCATGCC+TGG 0.405137 1_1:+68852712 Msa0039300:CDS
GAATCTTTGGTCATGAGAAA+TGG 0.412406 1_1:+68852548 Msa0039300:CDS
GAGAGGAAAGTTCAGCAAAC+AGG 0.416883 1_1:+68851157 Msa0039300:CDS
GTTTGCTGAATGGCTTTCAT+TGG 0.418059 1_1:+68852490 Msa0039300:CDS
TTAAACATGGTCATGGTTGT+TGG 0.431623 1_1:+68850677 Msa0039300:exon
AAATTTGCAAATCAAATTGT+GGG 0.441722 1_1:+68852685 Msa0039300:CDS
ACCAAACATAGAAAAGGTTA+TGG 0.452214 1_1:+68850617 Msa0039300:five_prime_UTR
TAAATTTGCAAATCAAATTG+TGG 0.468216 1_1:+68852684 Msa0039300:CDS
AGTGGCCAAAGCTATGGAAA+TGG 0.470398 1_1:+68852322 Msa0039300:CDS
CTCACCCTTCATCACATGTT+AGG 0.480777 1_1:+68851194 Msa0039300:CDS
GAAACCAACCTGCCTTAATT+GGG 0.488214 1_1:-68850708 None:intergenic
GACAAAACCAAACATAGAAA+AGG 0.495774 1_1:+68850611 Msa0039300:five_prime_UTR
ATGCAAGAGGGACCATTCAA+TGG 0.504386 1_1:+68852605 Msa0039300:CDS
AAAGAGAGTGGCCAAAGCTA+TGG 0.515504 1_1:+68852316 Msa0039300:CDS
TTATGAAACTCTCCATTGAA+TGG 0.518250 1_1:-68852617 None:intergenic
AAAGTTCAGCAAACAGGAGG+AGG 0.533564 1_1:+68851163 Msa0039300:CDS
GTGATGTTGTTATAATTGTG+TGG 0.534849 1_1:-68852431 None:intergenic
AGCTCTGTCCCAATTAAGGC+AGG 0.541894 1_1:+68850700 Msa0039300:CDS
CATCTCAGTGGCTGAAATGT+TGG 0.543027 1_1:-68852648 None:intergenic
TTGTTACTTAAATCAACTCC+AGG 0.543581 1_1:-68852730 None:intergenic
CAGATAGCACAACATTTGCC+AGG 0.553213 1_1:+68852248 Msa0039300:intron
TGTTGCCTAACATGTGATGA+AGG 0.568808 1_1:-68851199 None:intergenic
AATCAACTCCAGGCATGCAA+TGG 0.573572 1_1:-68852720 None:intergenic
TAGCACAACATTTGCCAGGA+AGG 0.581725 1_1:+68852252 Msa0039300:CDS
TCAAGAAGCTACAATGCAAG+AGG 0.583454 1_1:+68852592 Msa0039300:CDS
TACATCCTTAAACATGGTCA+TGG 0.598095 1_1:+68850670 Msa0039300:exon
TTATTAGGCAAAACAAAATG+GGG 0.614230 1_1:+68850571 Msa0039300:five_prime_UTR
CTAAGGCCAGGATTAAAGAG+AGG 0.629264 1_1:+68851140 Msa0039300:intron
AGGAAAGTTCAGCAAACAGG+AGG 0.649041 1_1:+68851160 Msa0039300:CDS
CAAGAAGCTACAATGCAAGA+GGG 0.649433 1_1:+68852593 Msa0039300:CDS
AGAAACTACATCCTTAAACA+TGG 0.675410 1_1:+68850664 Msa0039300:exon
CTAACATGTGATGAAGGGTG+AGG 0.683728 1_1:-68851193 None:intergenic
GTTGCCTAACATGTGATGAA+GGG 0.702714 1_1:-68851198 None:intergenic
GCTGTGAATGAACATCTCAG+TGG 0.777293 1_1:-68852660 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGATAATTTTAAATAG+TGG - chr1_1:68851748-68851767 None:intergenic 10.0%
!! AGAATTAACTTAAATTATAT+TGG + chr1_1:68852214-68852233 Msa0039300:intron 10.0%
!!! TTTTAGATAATTTTAAATAG+TGG - chr1_1:68851748-68851767 None:intergenic 10.0%
!! AGAATTAACTTAAATTATAT+TGG + chr1_1:68852214-68852233 Msa0039300:intron 10.0%
!!! ATTTAAAGTGAAGTTTTATT+AGG + chr1_1:68850556-68850575 Msa0039300:five_prime_UTR 15.0%
!!! TTTAAGTTAGAGAATATAAT+TGG + chr1_1:68851320-68851339 Msa0039300:intron 15.0%
!! ATATTAAATCATCTATCAAA+TGG - chr1_1:68851722-68851741 None:intergenic 15.0%
!!! ATTTAAAGTGAAGTTTTATT+AGG + chr1_1:68850556-68850575 Msa0039300:five_prime_UTR 15.0%
!!! TTTAAGTTAGAGAATATAAT+TGG + chr1_1:68851320-68851339 Msa0039300:intron 15.0%
!! ATATTAAATCATCTATCAAA+TGG - chr1_1:68851722-68851741 None:intergenic 15.0%
!!! TTTTATTAGGCAAAACAAAA+TGG + chr1_1:68850569-68850588 Msa0039300:five_prime_UTR 20.0%
!! TTTATTAGGCAAAACAAAAT+GGG + chr1_1:68850570-68850589 Msa0039300:five_prime_UTR 20.0%
!! CCTACTTATAAATATGATTA+GGG - chr1_1:68850758-68850777 None:intergenic 20.0%
!! ACCTACTTATAAATATGATT+AGG - chr1_1:68850759-68850778 None:intergenic 20.0%
!!! TTGATTTTTGCTAAAATGTA+TGG + chr1_1:68850992-68851011 Msa0039300:intron 20.0%
!! TATTCAATTGTTCTATCTAA+TGG + chr1_1:68851047-68851066 Msa0039300:intron 20.0%
!!! TTTATTTTATAGAAGCAACT+AGG + chr1_1:68851372-68851391 Msa0039300:intron 20.0%
!! GGAAAAAAAAAAAAAAAGCA+TGG + chr1_1:68851406-68851425 Msa0039300:intron 20.0%
!!! TTTGTTTTCCTTTGTTTTTA+TGG + chr1_1:68851895-68851914 Msa0039300:intron 20.0%
!! TAACTTAAATTATATTGGTC+AGG + chr1_1:68852219-68852238 Msa0039300:intron 20.0%
!! ACAATGAGATAAAAAACTAT+TGG + chr1_1:68852279-68852298 Msa0039300:CDS 20.0%
!! TAAATTTGCAAATCAAATTG+TGG + chr1_1:68852684-68852703 Msa0039300:CDS 20.0%
!! AAATTTGCAAATCAAATTGT+GGG + chr1_1:68852685-68852704 Msa0039300:CDS 20.0%
!!! TTTTATTAGGCAAAACAAAA+TGG + chr1_1:68850569-68850588 Msa0039300:five_prime_UTR 20.0%
!! TTTATTAGGCAAAACAAAAT+GGG + chr1_1:68850570-68850589 Msa0039300:five_prime_UTR 20.0%
!! CCTACTTATAAATATGATTA+GGG - chr1_1:68850758-68850777 None:intergenic 20.0%
!! ACCTACTTATAAATATGATT+AGG - chr1_1:68850759-68850778 None:intergenic 20.0%
!!! TTGATTTTTGCTAAAATGTA+TGG + chr1_1:68850992-68851011 Msa0039300:intron 20.0%
!! TATTCAATTGTTCTATCTAA+TGG + chr1_1:68851047-68851066 Msa0039300:intron 20.0%
!!! TTTATTTTATAGAAGCAACT+AGG + chr1_1:68851372-68851391 Msa0039300:intron 20.0%
!! GGAAAAAAAAAAAAAAAGCA+TGG + chr1_1:68851406-68851425 Msa0039300:intron 20.0%
!!! TTTGTTTTCCTTTGTTTTTA+TGG + chr1_1:68851895-68851914 Msa0039300:intron 20.0%
!! TAACTTAAATTATATTGGTC+AGG + chr1_1:68852219-68852238 Msa0039300:intron 20.0%
!! ACAATGAGATAAAAAACTAT+TGG + chr1_1:68852279-68852298 Msa0039300:CDS 20.0%
!! TAAATTTGCAAATCAAATTG+TGG + chr1_1:68852684-68852703 Msa0039300:CDS 20.0%
!! AAATTTGCAAATCAAATTGT+GGG + chr1_1:68852685-68852704 Msa0039300:CDS 20.0%
! TTATTAGGCAAAACAAAATG+GGG + chr1_1:68850571-68850590 Msa0039300:five_prime_UTR 25.0%
! CCCTAATCATATTTATAAGT+AGG + chr1_1:68850755-68850774 Msa0039300:intron 25.0%
! TAAGAGTTTGAATCCTAAAA+TGG + chr1_1:68850823-68850842 Msa0039300:intron 25.0%
!! AATTCACTACTTTGAACTTT+TGG - chr1_1:68851288-68851307 None:intergenic 25.0%
! GTTAGAGAATATAATTGGAT+TGG + chr1_1:68851325-68851344 Msa0039300:intron 25.0%
!! CATGCTCTTCTTTTTAATAA+TGG + chr1_1:68851467-68851486 Msa0039300:intron 25.0%
! AATATACAGAATTGAGGAAT+AGG - chr1_1:68851520-68851539 None:intergenic 25.0%
! AGTTTGAATATACAGAATTG+AGG - chr1_1:68851526-68851545 None:intergenic 25.0%
! TCATTTATTTCCAATTACCA+AGG + chr1_1:68851657-68851676 Msa0039300:intron 25.0%
!! AAAAGTCTAATGATGATGAA+CGG + chr1_1:68851764-68851783 Msa0039300:intron 25.0%
!! TCTTTAGAAGTAGAAGTTTA+CGG + chr1_1:68851790-68851809 Msa0039300:intron 25.0%
! TGTTAGTTCCATAAAAACAA+AGG - chr1_1:68851906-68851925 None:intergenic 25.0%
!!! TCTTTTTTCCCTTTAAATGA+AGG + chr1_1:68852183-68852202 Msa0039300:intron 25.0%
! CTTATCATCCTTCATTTAAA+GGG - chr1_1:68852194-68852213 None:intergenic 25.0%
! TCTTATCATCCTTCATTTAA+AGG - chr1_1:68852195-68852214 None:intergenic 25.0%
! TTCATATTTGAAAAAGAGAG+TGG + chr1_1:68852304-68852323 Msa0039300:CDS 25.0%
! TACAAATTCAGTTGAATCTT+TGG + chr1_1:68852535-68852554 Msa0039300:CDS 25.0%
! TTATTAGGCAAAACAAAATG+GGG + chr1_1:68850571-68850590 Msa0039300:five_prime_UTR 25.0%
! CCCTAATCATATTTATAAGT+AGG + chr1_1:68850755-68850774 Msa0039300:intron 25.0%
! TAAGAGTTTGAATCCTAAAA+TGG + chr1_1:68850823-68850842 Msa0039300:intron 25.0%
!! AATTCACTACTTTGAACTTT+TGG - chr1_1:68851288-68851307 None:intergenic 25.0%
! GTTAGAGAATATAATTGGAT+TGG + chr1_1:68851325-68851344 Msa0039300:intron 25.0%
!! CATGCTCTTCTTTTTAATAA+TGG + chr1_1:68851467-68851486 Msa0039300:intron 25.0%
! AATATACAGAATTGAGGAAT+AGG - chr1_1:68851520-68851539 None:intergenic 25.0%
! AGTTTGAATATACAGAATTG+AGG - chr1_1:68851526-68851545 None:intergenic 25.0%
! TCATTTATTTCCAATTACCA+AGG + chr1_1:68851657-68851676 Msa0039300:intron 25.0%
!! AAAAGTCTAATGATGATGAA+CGG + chr1_1:68851764-68851783 Msa0039300:intron 25.0%
!! TCTTTAGAAGTAGAAGTTTA+CGG + chr1_1:68851790-68851809 Msa0039300:intron 25.0%
! TGTTAGTTCCATAAAAACAA+AGG - chr1_1:68851906-68851925 None:intergenic 25.0%
!!! TCTTTTTTCCCTTTAAATGA+AGG + chr1_1:68852183-68852202 Msa0039300:intron 25.0%
! CTTATCATCCTTCATTTAAA+GGG - chr1_1:68852194-68852213 None:intergenic 25.0%
! TCTTATCATCCTTCATTTAA+AGG - chr1_1:68852195-68852214 None:intergenic 25.0%
! TTCATATTTGAAAAAGAGAG+TGG + chr1_1:68852304-68852323 Msa0039300:CDS 25.0%
! TACAAATTCAGTTGAATCTT+TGG + chr1_1:68852535-68852554 Msa0039300:CDS 25.0%
!!! TTTGGTTTTGTCTTTTCTAG+TGG - chr1_1:68850603-68850622 None:intergenic 30.0%
GACAAAACCAAACATAGAAA+AGG + chr1_1:68850611-68850630 Msa0039300:five_prime_UTR 30.0%
ACCAAACATAGAAAAGGTTA+TGG + chr1_1:68850617-68850636 Msa0039300:five_prime_UTR 30.0%
! ACCATAACCTTTTCTATGTT+TGG - chr1_1:68850621-68850640 None:intergenic 30.0%
AGAAACTACATCCTTAAACA+TGG + chr1_1:68850664-68850683 Msa0039300:exon 30.0%
TTGGTTTCTTTCTTCTTCAA+AGG + chr1_1:68850723-68850742 Msa0039300:intron 30.0%
TCAACAGTTCTTAATAGCTA+AGG - chr1_1:68850976-68850995 None:intergenic 30.0%
GTACAATGTCATACATGTAT+AGG + chr1_1:68851071-68851090 Msa0039300:intron 30.0%
!! TAAGGTGGATTAACTATCTA+AGG + chr1_1:68851123-68851142 Msa0039300:intron 30.0%
! AGAGAATATAATTGGATTGG+AGG + chr1_1:68851328-68851347 Msa0039300:intron 30.0%
! GAGAATATAATTGGATTGGA+GGG + chr1_1:68851329-68851348 Msa0039300:intron 30.0%
! AAAAAAAAGCATGGCTTGAT+TGG + chr1_1:68851415-68851434 Msa0039300:intron 30.0%
TTCAAACTAAGCCTATTTCA+AGG + chr1_1:68851538-68851557 Msa0039300:intron 30.0%
AGAGATGAAATAAACCTATG+TGG + chr1_1:68851680-68851699 Msa0039300:intron 30.0%
ATGTCATCGTCTTAATAGTA+AGG - chr1_1:68851983-68852002 None:intergenic 30.0%
CTTAAATTATATTGGTCAGG+TGG + chr1_1:68852222-68852241 Msa0039300:intron 30.0%
GTGATGTTGTTATAATTGTG+TGG - chr1_1:68852434-68852453 None:intergenic 30.0%
!!! AGCAAACAATAGTTTTGGTA+AGG - chr1_1:68852477-68852496 None:intergenic 30.0%
! CATTCAGCAAACAATAGTTT+TGG - chr1_1:68852482-68852501 None:intergenic 30.0%
! CAAAACTATTGTTTGCTGAA+TGG + chr1_1:68852480-68852499 Msa0039300:CDS 30.0%
TTATGAAACTCTCCATTGAA+TGG - chr1_1:68852620-68852639 None:intergenic 30.0%
CAAATCAAATTGTGGGAAAT+GGG + chr1_1:68852692-68852711 Msa0039300:CDS 30.0%
!!! TTTGGTTTTGTCTTTTCTAG+TGG - chr1_1:68850603-68850622 None:intergenic 30.0%
GACAAAACCAAACATAGAAA+AGG + chr1_1:68850611-68850630 Msa0039300:five_prime_UTR 30.0%
ACCAAACATAGAAAAGGTTA+TGG + chr1_1:68850617-68850636 Msa0039300:five_prime_UTR 30.0%
! ACCATAACCTTTTCTATGTT+TGG - chr1_1:68850621-68850640 None:intergenic 30.0%
AGAAACTACATCCTTAAACA+TGG + chr1_1:68850664-68850683 Msa0039300:exon 30.0%
TTGGTTTCTTTCTTCTTCAA+AGG + chr1_1:68850723-68850742 Msa0039300:intron 30.0%
TCAACAGTTCTTAATAGCTA+AGG - chr1_1:68850976-68850995 None:intergenic 30.0%
GTACAATGTCATACATGTAT+AGG + chr1_1:68851071-68851090 Msa0039300:intron 30.0%
!! TAAGGTGGATTAACTATCTA+AGG + chr1_1:68851123-68851142 Msa0039300:intron 30.0%
! AGAGAATATAATTGGATTGG+AGG + chr1_1:68851328-68851347 Msa0039300:intron 30.0%
! GAGAATATAATTGGATTGGA+GGG + chr1_1:68851329-68851348 Msa0039300:intron 30.0%
! AAAAAAAAGCATGGCTTGAT+TGG + chr1_1:68851415-68851434 Msa0039300:intron 30.0%
TTCAAACTAAGCCTATTTCA+AGG + chr1_1:68851538-68851557 Msa0039300:intron 30.0%
AGAGATGAAATAAACCTATG+TGG + chr1_1:68851680-68851699 Msa0039300:intron 30.0%
ATGTCATCGTCTTAATAGTA+AGG - chr1_1:68851983-68852002 None:intergenic 30.0%
CTTAAATTATATTGGTCAGG+TGG + chr1_1:68852222-68852241 Msa0039300:intron 30.0%
GTGATGTTGTTATAATTGTG+TGG - chr1_1:68852434-68852453 None:intergenic 30.0%
!!! AGCAAACAATAGTTTTGGTA+AGG - chr1_1:68852477-68852496 None:intergenic 30.0%
! CATTCAGCAAACAATAGTTT+TGG - chr1_1:68852482-68852501 None:intergenic 30.0%
! CAAAACTATTGTTTGCTGAA+TGG + chr1_1:68852480-68852499 Msa0039300:CDS 30.0%
TTATGAAACTCTCCATTGAA+TGG - chr1_1:68852620-68852639 None:intergenic 30.0%
CAAATCAAATTGTGGGAAAT+GGG + chr1_1:68852692-68852711 Msa0039300:CDS 30.0%
TACATCCTTAAACATGGTCA+TGG + chr1_1:68850670-68850689 Msa0039300:exon 35.0%
AACAACCATGACCATGTTTA+AGG - chr1_1:68850678-68850697 None:intergenic 35.0%
TTAAACATGGTCATGGTTGT+TGG + chr1_1:68850677-68850696 Msa0039300:exon 35.0%
!! ATTTAAAGTTTTTGCCCGTG+AGG + chr1_1:68850786-68850805 Msa0039300:intron 35.0%
!! GTGACATTGATGACCATTTT+AGG - chr1_1:68850839-68850858 None:intergenic 35.0%
ATACATGTATAGGCTTGCAA+AGG + chr1_1:68851081-68851100 Msa0039300:intron 35.0%
AACTTTCCTCTCTTTAATCC+TGG - chr1_1:68851149-68851168 None:intergenic 35.0%
AGCAACTAGGCTGTATTTAA+TGG + chr1_1:68851385-68851404 Msa0039300:intron 35.0%
GGAACACATAACCTTGAAAT+AGG - chr1_1:68851552-68851571 None:intergenic 35.0%
GTGAAGGAAAACTCTATAGA+AGG - chr1_1:68851573-68851592 None:intergenic 35.0%
! CTACTGATTGAAGTTTGTGA+AGG - chr1_1:68851589-68851608 None:intergenic 35.0%
! TTTCATCTCTCCTTGGTAAT+TGG - chr1_1:68851670-68851689 None:intergenic 35.0%
AGGTTTATTTCATCTCTCCT+TGG - chr1_1:68851677-68851696 None:intergenic 35.0%
TAAACCTATGTGGTGAGATT+TGG + chr1_1:68851690-68851709 Msa0039300:intron 35.0%
AAACCTATGTGGTGAGATTT+GGG + chr1_1:68851691-68851710 Msa0039300:intron 35.0%
CTCCTCATGTTTACTATGAT+CGG + chr1_1:68851815-68851834 Msa0039300:intron 35.0%
ATGTTTACTATGATCGGTGT+TGG + chr1_1:68851821-68851840 Msa0039300:intron 35.0%
AAACACCATCGATCATCATT+GGG - chr1_1:68851929-68851948 None:intergenic 35.0%
AAAACACCATCGATCATCAT+TGG - chr1_1:68851930-68851949 None:intergenic 35.0%
! GAAGTTTAAGATTCTCCTTG+TGG - chr1_1:68852121-68852140 None:intergenic 35.0%
TGATTCCATTTCCATAGCTT+TGG - chr1_1:68852330-68852349 None:intergenic 35.0%
!! GTTGCAAGTTTTTGAAGAGA+GGG - chr1_1:68852404-68852423 None:intergenic 35.0%
!! TGTTGCAAGTTTTTGAAGAG+AGG - chr1_1:68852405-68852424 None:intergenic 35.0%
GAATCTTTGGTCATGAGAAA+TGG + chr1_1:68852548-68852567 Msa0039300:CDS 35.0%
GCAAATCAAATTGTGGGAAA+TGG + chr1_1:68852691-68852710 Msa0039300:CDS 35.0%
TACATCCTTAAACATGGTCA+TGG + chr1_1:68850670-68850689 Msa0039300:exon 35.0%
AACAACCATGACCATGTTTA+AGG - chr1_1:68850678-68850697 None:intergenic 35.0%
TTAAACATGGTCATGGTTGT+TGG + chr1_1:68850677-68850696 Msa0039300:exon 35.0%
!! ATTTAAAGTTTTTGCCCGTG+AGG + chr1_1:68850786-68850805 Msa0039300:intron 35.0%
!! GTGACATTGATGACCATTTT+AGG - chr1_1:68850839-68850858 None:intergenic 35.0%
ATACATGTATAGGCTTGCAA+AGG + chr1_1:68851081-68851100 Msa0039300:intron 35.0%
AACTTTCCTCTCTTTAATCC+TGG - chr1_1:68851149-68851168 None:intergenic 35.0%
AGCAACTAGGCTGTATTTAA+TGG + chr1_1:68851385-68851404 Msa0039300:intron 35.0%
GGAACACATAACCTTGAAAT+AGG - chr1_1:68851552-68851571 None:intergenic 35.0%
GTGAAGGAAAACTCTATAGA+AGG - chr1_1:68851573-68851592 None:intergenic 35.0%
! CTACTGATTGAAGTTTGTGA+AGG - chr1_1:68851589-68851608 None:intergenic 35.0%
! TTTCATCTCTCCTTGGTAAT+TGG - chr1_1:68851670-68851689 None:intergenic 35.0%
AGGTTTATTTCATCTCTCCT+TGG - chr1_1:68851677-68851696 None:intergenic 35.0%
TAAACCTATGTGGTGAGATT+TGG + chr1_1:68851690-68851709 Msa0039300:intron 35.0%
AAACCTATGTGGTGAGATTT+GGG + chr1_1:68851691-68851710 Msa0039300:intron 35.0%
CTCCTCATGTTTACTATGAT+CGG + chr1_1:68851815-68851834 Msa0039300:intron 35.0%
ATGTTTACTATGATCGGTGT+TGG + chr1_1:68851821-68851840 Msa0039300:intron 35.0%
AAACACCATCGATCATCATT+GGG - chr1_1:68851929-68851948 None:intergenic 35.0%
AAAACACCATCGATCATCAT+TGG - chr1_1:68851930-68851949 None:intergenic 35.0%
! GAAGTTTAAGATTCTCCTTG+TGG - chr1_1:68852121-68852140 None:intergenic 35.0%
TGATTCCATTTCCATAGCTT+TGG - chr1_1:68852330-68852349 None:intergenic 35.0%
!! GTTGCAAGTTTTTGAAGAGA+GGG - chr1_1:68852404-68852423 None:intergenic 35.0%
!! TGTTGCAAGTTTTTGAAGAG+AGG - chr1_1:68852405-68852424 None:intergenic 35.0%
GAATCTTTGGTCATGAGAAA+TGG + chr1_1:68852548-68852567 Msa0039300:CDS 35.0%
GCAAATCAAATTGTGGGAAA+TGG + chr1_1:68852691-68852710 Msa0039300:CDS 35.0%
!! GTTTTGTCTTTTCTAGTGGC+TGG - chr1_1:68850599-68850618 None:intergenic 40.0%
CTGAGTTTGTGATCTTCTTC+AGG - chr1_1:68850646-68850665 None:intergenic 40.0%
GAAACCAACCTGCCTTAATT+GGG - chr1_1:68850711-68850730 None:intergenic 40.0%
AGAAACCAACCTGCCTTAAT+TGG - chr1_1:68850712-68850731 None:intergenic 40.0%
TGTATAGGCTTGCAAAGGAA+TGG + chr1_1:68851086-68851105 Msa0039300:intron 40.0%
ATGGAAAAAGCTGCAGACTA+AGG + chr1_1:68851105-68851124 Msa0039300:intron 40.0%
! TGGATTAACTATCTAAGGCC+AGG + chr1_1:68851128-68851147 Msa0039300:intron 40.0%
GTTGCCTAACATGTGATGAA+GGG - chr1_1:68851201-68851220 None:intergenic 40.0%
TGTTGCCTAACATGTGATGA+AGG - chr1_1:68851202-68851221 None:intergenic 40.0%
CTTGATTGGTAAGTCAAACC+AGG + chr1_1:68851429-68851448 Msa0039300:intron 40.0%
TGGTAAGTCAAACCAGGAAT+TGG + chr1_1:68851435-68851454 Msa0039300:intron 40.0%
AACCTATGTGGTGAGATTTG+GGG + chr1_1:68851692-68851711 Msa0039300:intron 40.0%
CACCGATCATAGTAAACATG+AGG - chr1_1:68851820-68851839 None:intergenic 40.0%
TAACACCCAATGATGATCGA+TGG + chr1_1:68851921-68851940 Msa0039300:intron 40.0%
TCGTCTTAATAGTAAGGACG+AGG - chr1_1:68851977-68851996 None:intergenic 40.0%
! GTTTGCTGAATGGCTTTCAT+TGG + chr1_1:68852490-68852509 Msa0039300:CDS 40.0%
TCAAGAAGCTACAATGCAAG+AGG + chr1_1:68852592-68852611 Msa0039300:CDS 40.0%
CAAGAAGCTACAATGCAAGA+GGG + chr1_1:68852593-68852612 Msa0039300:CDS 40.0%
!! GTTTTGTCTTTTCTAGTGGC+TGG - chr1_1:68850599-68850618 None:intergenic 40.0%
CTGAGTTTGTGATCTTCTTC+AGG - chr1_1:68850646-68850665 None:intergenic 40.0%
GAAACCAACCTGCCTTAATT+GGG - chr1_1:68850711-68850730 None:intergenic 40.0%
AGAAACCAACCTGCCTTAAT+TGG - chr1_1:68850712-68850731 None:intergenic 40.0%
TGTATAGGCTTGCAAAGGAA+TGG + chr1_1:68851086-68851105 Msa0039300:intron 40.0%
ATGGAAAAAGCTGCAGACTA+AGG + chr1_1:68851105-68851124 Msa0039300:intron 40.0%
! TGGATTAACTATCTAAGGCC+AGG + chr1_1:68851128-68851147 Msa0039300:intron 40.0%
GTTGCCTAACATGTGATGAA+GGG - chr1_1:68851201-68851220 None:intergenic 40.0%
TGTTGCCTAACATGTGATGA+AGG - chr1_1:68851202-68851221 None:intergenic 40.0%
CTTGATTGGTAAGTCAAACC+AGG + chr1_1:68851429-68851448 Msa0039300:intron 40.0%
TGGTAAGTCAAACCAGGAAT+TGG + chr1_1:68851435-68851454 Msa0039300:intron 40.0%
AACCTATGTGGTGAGATTTG+GGG + chr1_1:68851692-68851711 Msa0039300:intron 40.0%
CACCGATCATAGTAAACATG+AGG - chr1_1:68851820-68851839 None:intergenic 40.0%
TAACACCCAATGATGATCGA+TGG + chr1_1:68851921-68851940 Msa0039300:intron 40.0%
TCGTCTTAATAGTAAGGACG+AGG - chr1_1:68851977-68851996 None:intergenic 40.0%
! GTTTGCTGAATGGCTTTCAT+TGG + chr1_1:68852490-68852509 Msa0039300:CDS 40.0%
TCAAGAAGCTACAATGCAAG+AGG + chr1_1:68852592-68852611 Msa0039300:CDS 40.0%
CAAGAAGCTACAATGCAAGA+GGG + chr1_1:68852593-68852612 Msa0039300:CDS 40.0%
TTGGAGCTCTGTCCCAATTA+AGG + chr1_1:68850696-68850715 Msa0039300:CDS 45.0%
ACTACAAAGCTGACACCTCA+CGG - chr1_1:68850804-68850823 None:intergenic 45.0%
GAAAAAGCTGCAGACTAAGG+TGG + chr1_1:68851108-68851127 Msa0039300:intron 45.0%
CTAAGGCCAGGATTAAAGAG+AGG + chr1_1:68851140-68851159 Msa0039300:intron 45.0%
GAGAGGAAAGTTCAGCAAAC+AGG + chr1_1:68851157-68851176 Msa0039300:CDS 45.0%
AGGAAAGTTCAGCAAACAGG+AGG + chr1_1:68851160-68851179 Msa0039300:CDS 45.0%
AAAGTTCAGCAAACAGGAGG+AGG + chr1_1:68851163-68851182 Msa0039300:CDS 45.0%
CTAACATGTGATGAAGGGTG+AGG - chr1_1:68851196-68851215 None:intergenic 45.0%
CTCACCCTTCATCACATGTT+AGG + chr1_1:68851194-68851213 Msa0039300:CDS 45.0%
AGTCAAACCAGGAATTGGCT+TGG + chr1_1:68851440-68851459 Msa0039300:intron 45.0%
ATGCTAACCAAGCCAATTCC+TGG - chr1_1:68851450-68851469 None:intergenic 45.0%
GACTATGACGCAATAGGTGT+TGG - chr1_1:68852094-68852113 None:intergenic 45.0%
GGTTGAGACTATGACGCAAT+AGG - chr1_1:68852100-68852119 None:intergenic 45.0%
CAGATAGCACAACATTTGCC+AGG + chr1_1:68852248-68852267 Msa0039300:intron 45.0%
TAGCACAACATTTGCCAGGA+AGG + chr1_1:68852252-68852271 Msa0039300:CDS 45.0%
ATCTCATTGTCTGTCCTTCC+TGG - chr1_1:68852269-68852288 None:intergenic 45.0%
AAAGAGAGTGGCCAAAGCTA+TGG + chr1_1:68852316-68852335 Msa0039300:CDS 45.0%
AGTGGCCAAAGCTATGGAAA+TGG + chr1_1:68852322-68852341 Msa0039300:CDS 45.0%
GGTGCTTTGATGTGTCTCTT+TGG - chr1_1:68852456-68852475 None:intergenic 45.0%
ATGCAAGAGGGACCATTCAA+TGG + chr1_1:68852605-68852624 Msa0039300:CDS 45.0%
CATCTCAGTGGCTGAAATGT+TGG - chr1_1:68852651-68852670 None:intergenic 45.0%
GCTGTGAATGAACATCTCAG+TGG - chr1_1:68852663-68852682 None:intergenic 45.0%
AATCAACTCCAGGCATGCAA+TGG - chr1_1:68852723-68852742 None:intergenic 45.0%
TTGGAGCTCTGTCCCAATTA+AGG + chr1_1:68850696-68850715 Msa0039300:CDS 45.0%
ACTACAAAGCTGACACCTCA+CGG - chr1_1:68850804-68850823 None:intergenic 45.0%
GAAAAAGCTGCAGACTAAGG+TGG + chr1_1:68851108-68851127 Msa0039300:intron 45.0%
CTAAGGCCAGGATTAAAGAG+AGG + chr1_1:68851140-68851159 Msa0039300:intron 45.0%
GAGAGGAAAGTTCAGCAAAC+AGG + chr1_1:68851157-68851176 Msa0039300:CDS 45.0%
AGGAAAGTTCAGCAAACAGG+AGG + chr1_1:68851160-68851179 Msa0039300:CDS 45.0%
AAAGTTCAGCAAACAGGAGG+AGG + chr1_1:68851163-68851182 Msa0039300:CDS 45.0%
CTAACATGTGATGAAGGGTG+AGG - chr1_1:68851196-68851215 None:intergenic 45.0%
CTCACCCTTCATCACATGTT+AGG + chr1_1:68851194-68851213 Msa0039300:CDS 45.0%
AGTCAAACCAGGAATTGGCT+TGG + chr1_1:68851440-68851459 Msa0039300:intron 45.0%
ATGCTAACCAAGCCAATTCC+TGG - chr1_1:68851450-68851469 None:intergenic 45.0%
GACTATGACGCAATAGGTGT+TGG - chr1_1:68852094-68852113 None:intergenic 45.0%
GGTTGAGACTATGACGCAAT+AGG - chr1_1:68852100-68852119 None:intergenic 45.0%
CAGATAGCACAACATTTGCC+AGG + chr1_1:68852248-68852267 Msa0039300:intron 45.0%
TAGCACAACATTTGCCAGGA+AGG + chr1_1:68852252-68852271 Msa0039300:CDS 45.0%
ATCTCATTGTCTGTCCTTCC+TGG - chr1_1:68852269-68852288 None:intergenic 45.0%
AAAGAGAGTGGCCAAAGCTA+TGG + chr1_1:68852316-68852335 Msa0039300:CDS 45.0%
AGTGGCCAAAGCTATGGAAA+TGG + chr1_1:68852322-68852341 Msa0039300:CDS 45.0%
GGTGCTTTGATGTGTCTCTT+TGG - chr1_1:68852456-68852475 None:intergenic 45.0%
ATGCAAGAGGGACCATTCAA+TGG + chr1_1:68852605-68852624 Msa0039300:CDS 45.0%
CATCTCAGTGGCTGAAATGT+TGG - chr1_1:68852651-68852670 None:intergenic 45.0%
GCTGTGAATGAACATCTCAG+TGG - chr1_1:68852663-68852682 None:intergenic 45.0%
AATCAACTCCAGGCATGCAA+TGG - chr1_1:68852723-68852742 None:intergenic 45.0%
AGCTCTGTCCCAATTAAGGC+AGG + chr1_1:68850700-68850719 Msa0039300:CDS 50.0%
CTGTCCCAATTAAGGCAGGT+TGG + chr1_1:68850704-68850723 Msa0039300:intron 50.0%
CTACAAAGCTGACACCTCAC+GGG - chr1_1:68850803-68850822 None:intergenic 50.0%
CACCCCAAATCTCACCACAT+AGG - chr1_1:68851697-68851716 None:intergenic 50.0%
CTATGTGGTGAGATTTGGGG+TGG + chr1_1:68851695-68851714 Msa0039300:intron 50.0%
GCGTCATAGTCTCAACCACA+AGG + chr1_1:68852103-68852122 Msa0039300:intron 50.0%
AGCTCTGTCCCAATTAAGGC+AGG + chr1_1:68850700-68850719 Msa0039300:CDS 50.0%
CTGTCCCAATTAAGGCAGGT+TGG + chr1_1:68850704-68850723 Msa0039300:intron 50.0%
CTACAAAGCTGACACCTCAC+GGG - chr1_1:68850803-68850822 None:intergenic 50.0%
CACCCCAAATCTCACCACAT+AGG - chr1_1:68851697-68851716 None:intergenic 50.0%
CTATGTGGTGAGATTTGGGG+TGG + chr1_1:68851695-68851714 Msa0039300:intron 50.0%
GCGTCATAGTCTCAACCACA+AGG + chr1_1:68852103-68852122 Msa0039300:intron 50.0%
GGGTTTGTCCATTGCATGCC+TGG + chr1_1:68852712-68852731 Msa0039300:CDS 55.0%
GGGTTTGTCCATTGCATGCC+TGG + chr1_1:68852712-68852731 Msa0039300:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 68850501 68852745 68850501 ID=Msa0039300;Name=Msa0039300
chr1_1 mRNA 68850501 68852745 68850501 ID=Msa0039300-mRNA-1;Parent=Msa0039300;Name=Msa0039300-mRNA-1;_AED=0.35;_eAED=0.38;_QI=182|0|0.33|1|1|1|3|0|203
chr1_1 exon 68850501 68850721 68850501 ID=Msa0039300-mRNA-1:exon:22516;Parent=Msa0039300-mRNA-1
chr1_1 exon 68851145 68851222 68851145 ID=Msa0039300-mRNA-1:exon:22517;Parent=Msa0039300-mRNA-1
chr1_1 exon 68852251 68852745 68852251 ID=Msa0039300-mRNA-1:exon:22518;Parent=Msa0039300-mRNA-1
chr1_1 five_prime_UTR 68850501 68850682 68850501 ID=Msa0039300-mRNA-1:five_prime_utr;Parent=Msa0039300-mRNA-1
chr1_1 CDS 68850683 68850721 68850683 ID=Msa0039300-mRNA-1:cds;Parent=Msa0039300-mRNA-1
chr1_1 CDS 68851145 68851222 68851145 ID=Msa0039300-mRNA-1:cds;Parent=Msa0039300-mRNA-1
chr1_1 CDS 68852251 68852745 68852251 ID=Msa0039300-mRNA-1:cds;Parent=Msa0039300-mRNA-1
Gene Sequence

>Msa0039300

ATGGTCATGGTTGTTGGAGCTCTGTCCCAATTAAGGCAGGCCAGGATTAAAGAGAGGAAAGTTCAGCAAACAGGAGGAGGAAACAATCCTCACCCTTCATCACATGTTAGGCAACAAATAGCACAACATTTGCCAGGAAGGACAGACAATGAGATAAAAAACTATTGGCATTCATATTTGAAAAAGAGAGTGGCCAAAGCTATGGAAATGGAATCACACAAACAATTTCAATATGCTAGCTCAAGCTCAGATACAATGAACTCTTCACCCTCTCTTCAAAAACTTGCAACACAAGATCCACACAATTATAACAACATCACCAAAGAGACACATCAAAGCACCTTACCAAAACTATTGTTTGCTGAATGGCTTTCATTGGATAATGTTAATAGTAGAAACTCTACAAATTCAGTTGAATCTTTGGTCATGAGAAATGGATTTGATCAAAATTCAGCTTTTCAAGAAGCTACAATGCAAGAGGGACCATTCAATGGAGAGTTTCATAATAGTGTGACCAACATTTCAGCCACTGAGATGTTCATTCACAGCTTAAATTTGCAAATCAAATTGTGGGAAATGGGTTTGTCCATTGCATGCCTGGAGTTGATTTAA
Protein sequence

>Msa0039300

MVMVVGALSQLRQARIKERKVQQTGGGNNPHPSSHVRQQIAQHLPGRTDNEIKNYWHSYLKKRVAKAMEMESHKQFQYASSSSDTMNSSPSLQKLATQDPHNYNNITKETHQSTLPKLLFAEWLSLDNVNSRNSTNSVESLVMRNGFDQNSAFQEATMQEGPFNGEFHNSVTNISATEMFIHSLNLQIKLWEMGLSIACLELI*