AlfalfaGEDB | Msa0039520

M. sativa cultivar zhongmu-4 / Msa0039520

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0039520	sp\|Q9SZL7\|FRS9_ARATH	25.939	293	194	3	187	478	203	473	1.11e-29	127

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0039520	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0039520	MtrunA17_Chr1g0203721	94.760	458	22	1	187	644	428	883	0.0	874
Msa0039520	MtrunA17_Chr1g0203721	93.750	192	12	0	1	192	1	192	1.30e-122	385
Msa0039520	MtrunA17_Chr6g0458391	44.907	216	112	1	168	383	558	766	1.71e-58	210
Msa0039520	MtrunA17_Chr6g0458391	51.562	128	60	1	59	186	221	346	2.19e-34	139
Msa0039520	MtrunA17_Chr6g0458391	43.353	173	87	4	22	190	50	215	1.98e-32	133
Msa0039520	MtrunA17_Chr3g0082121	40.293	273	154	3	174	445	385	649	1.69e-44	169
Msa0039520	MtrunA17_Chr3g0082121	53.684	95	43	1	69	162	49	143	1.39e-23	105
Msa0039520	MtrunA17_Chr2g0324011	32.093	215	145	1	187	400	372	586	1.56e-34	139
Msa0039520	MtrunA17_Chr5g0448511	27.027	259	188	1	187	444	201	459	1.47e-28	120
Msa0039520	MtrunA17_Chr5g0448761	26.712	292	209	4	187	476	429	717	7.66e-26	113

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0039520	AT4G38170.1	25.939	293	194	3	187	478	203	473	1.13e-30	127
Msa0039520	AT2G32250.2	29.825	285	182	8	187	463	358	632	5.84e-29	123
Msa0039520	AT2G32250.2	33.333	150	82	4	14	163	4	135	2.04e-11	67.8
Msa0039520	AT2G32250.4	29.825	285	182	8	187	463	358	632	5.84e-29	123
Msa0039520	AT2G32250.4	33.333	150	82	4	14	163	4	135	2.04e-11	67.8
Msa0039520	AT2G32250.6	29.825	285	182	8	187	463	358	632	5.86e-29	123
Msa0039520	AT2G32250.6	33.333	150	82	4	14	163	4	135	2.05e-11	67.8
Msa0039520	AT2G32250.1	29.825	285	182	8	187	463	358	632	5.86e-29	123
Msa0039520	AT2G32250.1	33.333	150	82	4	14	163	4	135	2.05e-11	67.8
Msa0039520	AT2G32250.3	29.825	285	182	8	187	463	358	632	5.86e-29	123

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Find 141 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCATGGAGAACTCTGT+TGG	0.577643	1_1:+69221041	None:intergenic
AAATTTACCTGCTCCAGAGA+GGG	0.588352	1_1:-69223406	Msa0039520:CDS
AACAACATGTTAGAATTGAT+TGG	0.361206	1_1:+69223229	None:intergenic
AACAAGTATGCCAGATTGAT+GGG	0.458593	1_1:-69223490	Msa0039520:CDS
AACATTGAACCCCATCAATC+TGG	0.425557	1_1:+69223480	None:intergenic
AAGAGCTTCTGAATCTGTGG+AGG	0.632101	1_1:-69221751	Msa0039520:CDS
AAGCAATAATCAGATGCAAC+AGG	0.498238	1_1:-69220885	Msa0039520:intron
AATAATGGCATTGCCAATAC+AGG	0.438574	1_1:+69220630	None:intergenic
AATATGAGCTGATGAATATT+TGG	0.194524	1_1:+69220521	None:intergenic
AATCTTGATATTGAAAAGTG+AGG	0.582977	1_1:-69221676	Msa0039520:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATGTAGAAAATGTTGATGT+TGG	-	chr1_1:69220470-69220489	Msa0039520:three_prime_UTR	30.0%
GGTTCAATGTTAGAAAAGAT+TGG	-	chr1_1:69220696-69220715	Msa0039520:CDS	30.0%
AGATTGGATTAATAGGAGTA+AGG	-	chr1_1:69220712-69220731	Msa0039520:CDS	30.0%
ATTAATAGGAGTAAGGTACA+TGG	-	chr1_1:69220719-69220738	Msa0039520:CDS	30.0%
GATTATGTTGTGCTTCAAAA+TGG	+	chr1_1:69220901-69220920	None:intergenic	30.0%
ACAATTATCTTCATTCTGTG+AGG	-	chr1_1:69221107-69221126	Msa0039520:CDS	30.0%
CAATTATCTTCATTCTGTGA+GGG	-	chr1_1:69221108-69221127	Msa0039520:CDS	30.0%
TGTATGGCATAAAAAAATGC+TGG	+	chr1_1:69221202-69221221	None:intergenic	30.0%
ACACCTAAAAACTGTACAAA+TGG	+	chr1_1:69221349-69221368	None:intergenic	30.0%
CAGCCAATTAAATGATTCAA+TGG	+	chr1_1:69221425-69221444	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	69220437	69223749	69220437	ID=Msa0039520;Name=Msa0039520
chr1_1	mRNA	69220437	69223749	69220437	ID=Msa0039520-mRNA-1;Parent=Msa0039520;Name=Msa0039520-mRNA-1;_AED=0.15;_eAED=0.25;_QI=0\|0.75\|0.6\|1\|1\|1\|5\|245\|644
chr1_1	exon	69220437	69220768	69220437	ID=Msa0039520-mRNA-1:exon:22689;Parent=Msa0039520-mRNA-1
chr1_1	exon	69220886	69220945	69220886	ID=Msa0039520-mRNA-1:exon:22688;Parent=Msa0039520-mRNA-1
chr1_1	exon	69221027	69221179	69221027	ID=Msa0039520-mRNA-1:exon:22687;Parent=Msa0039520-mRNA-1
chr1_1	exon	69221392	69222462	69221392	ID=Msa0039520-mRNA-1:exon:22686;Parent=Msa0039520-mRNA-1
chr1_1	exon	69223186	69223749	69223186	ID=Msa0039520-mRNA-1:exon:22685;Parent=Msa0039520-mRNA-1
chr1_1	CDS	69223186	69223749	69223186	ID=Msa0039520-mRNA-1:cds;Parent=Msa0039520-mRNA-1
chr1_1	CDS	69221392	69222462	69221392	ID=Msa0039520-mRNA-1:cds;Parent=Msa0039520-mRNA-1
chr1_1	CDS	69221027	69221179	69221027	ID=Msa0039520-mRNA-1:cds;Parent=Msa0039520-mRNA-1
chr1_1	CDS	69220886	69220945	69220886	ID=Msa0039520-mRNA-1:cds;Parent=Msa0039520-mRNA-1
chr1_1	CDS	69220682	69220768	69220682	ID=Msa0039520-mRNA-1:cds;Parent=Msa0039520-mRNA-1
chr1_1	three_prime_UTR	69220437	69220681	69220437	ID=Msa0039520-mRNA-1:three_prime_utr;Parent=Msa0039520-mRNA-1

Gene Sequence

>Msa0039520

ATGGATGGTGATCATAACAACTTAGGTAATGTGCATGTAGAAAATGTTGATGTTGGACTTGGAACATCCAATAGTTTGGACTTGAATGTGGACCAAAATTGTTGCAGTCCGAACGTTCTTCACGCTAATGGCTCTCAAACTCAATCTGGTAATGCATCAGCAAATGGAGGCTTTGTCAACACAGTGTTGGGAATCGGTACGGTATTTGAGTCTGATGAACATGCGTATAGATTCTATAACAAGTATGCCAGATTGATGGGGTTCAATGTTAGAAAAGATTGGATTAATAGGAGTAAGGTACATGGTATGGCGGTTTCTAGGAAATTTACCTGCTCCAGAGAGGGATATCGTAGGAAAGACAAGAGAGATTTTACTGTGAAGAAACATCGGAAGGAGACGAGAACTGGGTGTTTGGCACACATGATCATTACTCGTCAACAAGATGGAAAATACCAGGTTACCCATTTTGAAGCACAACATAATCATGATGATATAAACCCAATCAATTCTAACATGTTGTTACTAGAGTTGCAAAATGAATTTTGTGTTGCTCAAGCTGTGGAGCAAAATGAATGGTTGAGATGGATGTATAGAGAAAGGGAGAAATGGGCTGTCTGCTTTGGTCAGAATAGATTTTTTGTAGATGTCAAAGGCTTTCATTTAGGTGAGATGTTGTCTCACAAGCTTAGAAGCTATCTAAATTCTGACCTTGATGTAGTTCAATTTCTTAACCATTTTGAAAGGATAGTGGAGGAGCAACGATATAAAGAAATAGAAGCTAGTGATGAAATGAAGGGACGTCTTCCAAAACTAATGGGTAATGTGGTTGTGTTGAAACATGCTAGTGTTGCTTATACTCCAAGAGCATTTGAAATATTTCAGCAACGATATGAGAACTCTTTGAATGTTATTGTCAACCAGCACAAAAGAGATGGATATTTGTTTGAGTATAAAGTGAATACATTTGGGCATGCTAGACAGTACACTGTCACCTTCAATTCTTCAGATAATACAGTTGTCTGCAGTTGTATGAAGTTTGAGCATGTTGGCTTTCTTTGTAGCCATGCACTAAAAGTTCTTGATAATAGAAACATAAAAGTAGTTCCATCTCGATATATCTTGAAGAGATGGACAAAAGATGCAAGGTTGGGTAATGTCAGGGAGATTAAGCAATTTAAAATTCTAGATAACCCTAAGATGGTTGTAGCAAGCTGTTACAAAGATTTATGCCACAGACTTGTCAAGTTATCGGCAAGAGCTTCTGAATCTGTGGAGGCTTATCAGTTTGCTGTGAGGCAACTTGACGAAGTTATGGAAGGAGTGCAGAAAATCTTGATATTGAAAAGTGAGGAAGCTCAAGTTATCACCTCAAATAGTATTCATGTAGATGCTTCAGAAAGTGAGCCTGCGGTGATCTTTCCGAATGGACATGCGACTGAGGATCAGGATGAAAGTAACAGAGTAAGCGAAGAAATTGACCGGACCGCTACACCGGATAGGTGCCAACCAACGGTGAATTATAGTCAAACTGATTCAGATAGGATTTTAAATGTAGAACCTTCTTCAAATACTGTTGTCTGCATTTCAAGCCCCCCATCTCCATATGTTTCATCTCAACCAAATCTCCTTTTGCAGGGCTTATTTGGTTTTGAAACCAATGAAGTGGTTCAATGCATGTATGAACAGCCTGATCTTGTGTTGGATCATCAATCTAATACTAACCTGTTCCAACCGCCAAACTTCTATTCTAACCAACAGAGTTCTCCATGCCTTTCTCAACTATCGCAGGAGCCTATAATCCAAAACACATACCCTGAGTCCGTGCCAAGCAATAATCAGATGCAACAGGGTATGGATCTTGATATCCAAAATCCTCATTCAGAATCCTGTATCCTGCTGTGTGACCATAGATACCGAAGTTCTGACTCTGCATAA

Protein sequence

>Msa0039520

MDGDHNNLGNVHVENVDVGLGTSNSLDLNVDQNCCSPNVLHANGSQTQSGNASANGGFVNTVLGIGTVFESDEHAYRFYNKYARLMGFNVRKDWINRSKVHGMAVSRKFTCSREGYRRKDKRDFTVKKHRKETRTGCLAHMIITRQQDGKYQVTHFEAQHNHDDINPINSNMLLLELQNEFCVAQAVEQNEWLRWMYREREKWAVCFGQNRFFVDVKGFHLGEMLSHKLRSYLNSDLDVVQFLNHFERIVEEQRYKEIEASDEMKGRLPKLMGNVVVLKHASVAYTPRAFEIFQQRYENSLNVIVNQHKRDGYLFEYKVNTFGHARQYTVTFNSSDNTVVCSCMKFEHVGFLCSHALKVLDNRNIKVVPSRYILKRWTKDARLGNVREIKQFKILDNPKMVVASCYKDLCHRLVKLSARASESVEAYQFAVRQLDEVMEGVQKILILKSEEAQVITSNSIHVDASESEPAVIFPNGHATEDQDESNRVSEEIDRTATPDRCQPTVNYSQTDSDRILNVEPSSNTVVCISSPPSPYVSSQPNLLLQGLFGFETNEVVQCMYEQPDLVLDHQSNTNLFQPPNFYSNQQSSPCLSQLSQEPIIQNTYPESVPSNNQMQQGMDLDIQNPHSESCILLCDHRYRSSDSA*