AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0040250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0040250 MtrunA17_Chr1g0205001 92.500 40 3 0 30 69 186 225 1.08e-21 85.9
Msa0040250 MtrunA17_Chr1g0205031 76.471 34 8 0 31 64 419 452 2.30e-12 62.4
Msa0040250 MtrunA17_Chr5g0394661 70.968 31 9 0 31 61 502 532 7.06e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCACAAGCTCTTTGGTTT+TGG 0.211080 1_1:+70326807 None:intergenic
AGAACTGAATCTTCATTAAT+AGG 0.233906 1_1:+70327788 None:intergenic
GGCTTTCCGATTGATGAATA+AGG 0.277084 1_1:+70326860 None:intergenic
ATTTGATTCCACAAGCTCTT+TGG 0.335151 1_1:+70326801 None:intergenic
CCATTACCTTATTCATCAAT+CGG 0.402551 1_1:-70326866 Msa0040250:CDS
TGTTGTGAGTGCTTTGCCGC+TGG 0.450078 1_1:-70327857 Msa0040250:CDS
CTTGTGGAATCAAATGCTAT+TGG 0.456145 1_1:-70326793 Msa0040250:CDS
CCGATTGATGAATAAGGTAA+TGG 0.456999 1_1:+70326866 None:intergenic
GCCGCTGGAGTCTACTGTAC+AGG 0.525113 1_1:-70327842 Msa0040250:CDS
GAAGATTCAGTTCTGAAAGC+TGG 0.550155 1_1:-70327779 Msa0040250:CDS
ATTCAGTTCTGAAAGCTGGC+AGG 0.556614 1_1:-70327775 Msa0040250:CDS
GAATAAGGTAATGGAAGCAA+TGG 0.561489 1_1:+70326875 None:intergenic
ATCCAAAACCAAAGAGCTTG+TGG 0.570201 1_1:-70326809 Msa0040250:CDS
TCCTGTACAGTAGACTCCAG+CGG 0.695681 1_1:+70327841 None:intergenic
CAAAGCACTCACAACAACTG+AGG 0.698243 1_1:+70327864 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATATTTAGGATTACAAT+TGG + chr1_1:70327188-70327207 None:intergenic 20.0%
!! TCAACTTCAATTTCATATTT+AGG + chr1_1:70327199-70327218 None:intergenic 20.0%
!!! TTCATATTTAGGATTACAAT+TGG + chr1_1:70327188-70327207 None:intergenic 20.0%
!! TCAACTTCAATTTCATATTT+AGG + chr1_1:70327199-70327218 None:intergenic 20.0%
!! ATGTAAGCTTGACAAAATTT+TGG - chr1_1:70326823-70326842 Msa0040250:CDS 25.0%
!! TGTAAGCTTGACAAAATTTT+GGG - chr1_1:70326824-70326843 Msa0040250:CDS 25.0%
! TGCAAAAGAGTAAAAAAAGA+AGG + chr1_1:70326870-70326889 None:intergenic 25.0%
! AGAACTGAATCTTCATTAAT+AGG + chr1_1:70326979-70326998 None:intergenic 25.0%
!! TTACAATAGTAATGCTACTT+AGG - chr1_1:70327284-70327303 Msa0040250:intron 25.0%
! AATTTAAATTCAAGCATGCA+TGG + chr1_1:70327342-70327361 None:intergenic 25.0%
! GTTGTGATTATATGAAAAAG+CGG + chr1_1:70327695-70327714 None:intergenic 25.0%
!! TTTTTCATATAATCACAACC+AGG - chr1_1:70327696-70327715 Msa0040250:intron 25.0%
! ACACTTAACATTTACAACTT+GGG + chr1_1:70327765-70327784 None:intergenic 25.0%
!! AATTAAGCACAAGATCTTTT+CGG + chr1_1:70327928-70327947 None:intergenic 25.0%
!!! AGATCTTGTGCTTAATTTTT+TGG - chr1_1:70327931-70327950 Msa0040250:intron 25.0%
!! ATGTAAGCTTGACAAAATTT+TGG - chr1_1:70326823-70326842 Msa0040250:CDS 25.0%
!! TGTAAGCTTGACAAAATTTT+GGG - chr1_1:70326824-70326843 Msa0040250:CDS 25.0%
! TGCAAAAGAGTAAAAAAAGA+AGG + chr1_1:70326870-70326889 None:intergenic 25.0%
! AGAACTGAATCTTCATTAAT+AGG + chr1_1:70326979-70326998 None:intergenic 25.0%
!! TTACAATAGTAATGCTACTT+AGG - chr1_1:70327284-70327303 Msa0040250:intron 25.0%
! AATTTAAATTCAAGCATGCA+TGG + chr1_1:70327342-70327361 None:intergenic 25.0%
! GTTGTGATTATATGAAAAAG+CGG + chr1_1:70327695-70327714 None:intergenic 25.0%
!! TTTTTCATATAATCACAACC+AGG - chr1_1:70327696-70327715 Msa0040250:intron 25.0%
! ACACTTAACATTTACAACTT+GGG + chr1_1:70327765-70327784 None:intergenic 25.0%
!! AATTAAGCACAAGATCTTTT+CGG + chr1_1:70327928-70327947 None:intergenic 25.0%
!!! AGATCTTGTGCTTAATTTTT+TGG - chr1_1:70327931-70327950 Msa0040250:intron 25.0%
!! AATAACACAAGTATTGATGC+TGG + chr1_1:70327152-70327171 None:intergenic 30.0%
TAACCACAATGGATAATGAT+CGG - chr1_1:70327402-70327421 Msa0040250:intron 30.0%
AGCAAAATTAGATAGAGCTA+TGG + chr1_1:70327491-70327510 None:intergenic 30.0%
CACTTAACATTTACAACTTG+GGG + chr1_1:70327764-70327783 None:intergenic 30.0%
CACACTTAACATTTACAACT+TGG + chr1_1:70327766-70327785 None:intergenic 30.0%
TAAGTGTGCGAAAATATACA+TGG - chr1_1:70327778-70327797 Msa0040250:CDS 30.0%
CCATTACCTTATTCATCAAT+CGG - chr1_1:70327898-70327917 Msa0040250:CDS 30.0%
!! AATAACACAAGTATTGATGC+TGG + chr1_1:70327152-70327171 None:intergenic 30.0%
TAACCACAATGGATAATGAT+CGG - chr1_1:70327402-70327421 Msa0040250:intron 30.0%
AGCAAAATTAGATAGAGCTA+TGG + chr1_1:70327491-70327510 None:intergenic 30.0%
CACTTAACATTTACAACTTG+GGG + chr1_1:70327764-70327783 None:intergenic 30.0%
CACACTTAACATTTACAACT+TGG + chr1_1:70327766-70327785 None:intergenic 30.0%
TAAGTGTGCGAAAATATACA+TGG - chr1_1:70327778-70327797 Msa0040250:CDS 30.0%
CCATTACCTTATTCATCAAT+CGG - chr1_1:70327898-70327917 Msa0040250:CDS 30.0%
! ATTTCCCTAGATCTTGGTTT+TGG + chr1_1:70327051-70327070 None:intergenic 35.0%
! CATTTGATTTCCCTAGATCT+TGG + chr1_1:70327057-70327076 None:intergenic 35.0%
CTAGGGAAATCAAATGCTAT+TGG - chr1_1:70327061-70327080 Msa0040250:intron 35.0%
TTATCTCCTAGTTACAGTGA+TGG - chr1_1:70327220-70327239 Msa0040250:intron 35.0%
TAAATTCAAGCATGCATGGT+TGG + chr1_1:70327338-70327357 None:intergenic 35.0%
GAACAGATCATAGCTCAAAT+TGG + chr1_1:70327376-70327395 None:intergenic 35.0%
ATTTGAGCTATGATCTGTTC+AGG - chr1_1:70327376-70327395 Msa0040250:intron 35.0%
GTCAACTATTTCCTAATGCT+CGG + chr1_1:70327453-70327472 None:intergenic 35.0%
! ATTCCATATGATGGAGAGAT+TGG - chr1_1:70327580-70327599 Msa0040250:intron 35.0%
CATTTGTAACAACATGCATG+AGG + chr1_1:70327661-70327680 None:intergenic 35.0%
TCATATAATCACAACCAGGT+CGG - chr1_1:70327700-70327719 Msa0040250:intron 35.0%
TGTGCGAAAATATACATGGT+AGG - chr1_1:70327782-70327801 Msa0040250:CDS 35.0%
CGAAAATATACATGGTAGGA+AGG - chr1_1:70327786-70327805 Msa0040250:CDS 35.0%
!! GAATAAGGTAATGGAAGCAA+TGG + chr1_1:70327892-70327911 None:intergenic 35.0%
CCGATTGATGAATAAGGTAA+TGG + chr1_1:70327901-70327920 None:intergenic 35.0%
ATTTGATTCCACAAGCTCTT+TGG + chr1_1:70327966-70327985 None:intergenic 35.0%
CTTGTGGAATCAAATGCTAT+TGG - chr1_1:70327971-70327990 Msa0040250:CDS 35.0%
! ATTTCCCTAGATCTTGGTTT+TGG + chr1_1:70327051-70327070 None:intergenic 35.0%
! CATTTGATTTCCCTAGATCT+TGG + chr1_1:70327057-70327076 None:intergenic 35.0%
CTAGGGAAATCAAATGCTAT+TGG - chr1_1:70327061-70327080 Msa0040250:intron 35.0%
TTATCTCCTAGTTACAGTGA+TGG - chr1_1:70327220-70327239 Msa0040250:intron 35.0%
TAAATTCAAGCATGCATGGT+TGG + chr1_1:70327338-70327357 None:intergenic 35.0%
GAACAGATCATAGCTCAAAT+TGG + chr1_1:70327376-70327395 None:intergenic 35.0%
ATTTGAGCTATGATCTGTTC+AGG - chr1_1:70327376-70327395 Msa0040250:intron 35.0%
GTCAACTATTTCCTAATGCT+CGG + chr1_1:70327453-70327472 None:intergenic 35.0%
! ATTCCATATGATGGAGAGAT+TGG - chr1_1:70327580-70327599 Msa0040250:intron 35.0%
CATTTGTAACAACATGCATG+AGG + chr1_1:70327661-70327680 None:intergenic 35.0%
TCATATAATCACAACCAGGT+CGG - chr1_1:70327700-70327719 Msa0040250:intron 35.0%
TGTGCGAAAATATACATGGT+AGG - chr1_1:70327782-70327801 Msa0040250:CDS 35.0%
CGAAAATATACATGGTAGGA+AGG - chr1_1:70327786-70327805 Msa0040250:CDS 35.0%
!! GAATAAGGTAATGGAAGCAA+TGG + chr1_1:70327892-70327911 None:intergenic 35.0%
CCGATTGATGAATAAGGTAA+TGG + chr1_1:70327901-70327920 None:intergenic 35.0%
ATTTGATTCCACAAGCTCTT+TGG + chr1_1:70327966-70327985 None:intergenic 35.0%
CTTGTGGAATCAAATGCTAT+TGG - chr1_1:70327971-70327990 Msa0040250:CDS 35.0%
!! GAAGATTCAGTTCTGAAAGC+TGG - chr1_1:70326985-70327004 Msa0040250:intron 40.0%
TGTTCAGGAACTAACCACAA+TGG - chr1_1:70327391-70327410 Msa0040250:intron 40.0%
CTTCCGATCATTATCCATTG+TGG + chr1_1:70327408-70327427 None:intergenic 40.0%
CAATGGATAATGATCGGAAG+TGG - chr1_1:70327408-70327427 Msa0040250:intron 40.0%
ACTATTTCCTAATGCTCGGT+TGG + chr1_1:70327449-70327468 None:intergenic 40.0%
GAGCTATGGAAAAAGAAGCT+TGG + chr1_1:70327477-70327496 None:intergenic 40.0%
GCTGATGAGATTCCATATGA+TGG - chr1_1:70327571-70327590 Msa0040250:intron 40.0%
GGCTTTCCGATTGATGAATA+AGG + chr1_1:70327907-70327926 None:intergenic 40.0%
ATCCAAAACCAAAGAGCTTG+TGG - chr1_1:70327955-70327974 Msa0040250:intron 40.0%
!! TTCCACAAGCTCTTTGGTTT+TGG + chr1_1:70327960-70327979 None:intergenic 40.0%
!! GAAGATTCAGTTCTGAAAGC+TGG - chr1_1:70326985-70327004 Msa0040250:intron 40.0%
TGTTCAGGAACTAACCACAA+TGG - chr1_1:70327391-70327410 Msa0040250:intron 40.0%
CTTCCGATCATTATCCATTG+TGG + chr1_1:70327408-70327427 None:intergenic 40.0%
CAATGGATAATGATCGGAAG+TGG - chr1_1:70327408-70327427 Msa0040250:intron 40.0%
ACTATTTCCTAATGCTCGGT+TGG + chr1_1:70327449-70327468 None:intergenic 40.0%
GAGCTATGGAAAAAGAAGCT+TGG + chr1_1:70327477-70327496 None:intergenic 40.0%
GCTGATGAGATTCCATATGA+TGG - chr1_1:70327571-70327590 Msa0040250:intron 40.0%
GGCTTTCCGATTGATGAATA+AGG + chr1_1:70327907-70327926 None:intergenic 40.0%
ATCCAAAACCAAAGAGCTTG+TGG - chr1_1:70327955-70327974 Msa0040250:intron 40.0%
!! TTCCACAAGCTCTTTGGTTT+TGG + chr1_1:70327960-70327979 None:intergenic 40.0%
! CAAAGCACTCACAACAACTG+AGG + chr1_1:70326903-70326922 None:intergenic 45.0%
!! ATTCAGTTCTGAAAGCTGGC+AGG - chr1_1:70326989-70327008 Msa0040250:intron 45.0%
!! GTATGTGACAGAGCGTTGTT+TGG - chr1_1:70327022-70327041 Msa0040250:intron 45.0%
GCAGCCAAAACCAAGATCTA+GGG - chr1_1:70327044-70327063 Msa0040250:intron 45.0%
TTCCTGCCATCACTGTAACT+AGG + chr1_1:70327229-70327248 None:intergenic 45.0%
GGCAGGAACTCATTAGAATG+TGG - chr1_1:70327241-70327260 Msa0040250:intron 45.0%
ATTCAAGCATGCATGGTTGG+TGG + chr1_1:70327335-70327354 None:intergenic 45.0%
!! TGACAAAGCTCGGTGCAAAA+AGG + chr1_1:70327527-70327546 None:intergenic 45.0%
GGTGACAACATGACAAAGCT+CGG + chr1_1:70327537-70327556 None:intergenic 45.0%
TTGTCATGTTGTCACCTTGC+TGG - chr1_1:70327541-70327560 Msa0040250:intron 45.0%
TTGTCACCTTGCTGGCAAAT+AGG - chr1_1:70327549-70327568 Msa0040250:intron 45.0%
AGGCCAATCTCTCCATCATA+TGG + chr1_1:70327586-70327605 None:intergenic 45.0%
CAAAGGAGTGACAATCACAG+AGG - chr1_1:70327623-70327642 Msa0040250:intron 45.0%
! CAAAGCACTCACAACAACTG+AGG + chr1_1:70326903-70326922 None:intergenic 45.0%
!! ATTCAGTTCTGAAAGCTGGC+AGG - chr1_1:70326989-70327008 Msa0040250:intron 45.0%
!! GTATGTGACAGAGCGTTGTT+TGG - chr1_1:70327022-70327041 Msa0040250:intron 45.0%
GCAGCCAAAACCAAGATCTA+GGG - chr1_1:70327044-70327063 Msa0040250:intron 45.0%
TTCCTGCCATCACTGTAACT+AGG + chr1_1:70327229-70327248 None:intergenic 45.0%
GGCAGGAACTCATTAGAATG+TGG - chr1_1:70327241-70327260 Msa0040250:intron 45.0%
ATTCAAGCATGCATGGTTGG+TGG + chr1_1:70327335-70327354 None:intergenic 45.0%
!! TGACAAAGCTCGGTGCAAAA+AGG + chr1_1:70327527-70327546 None:intergenic 45.0%
GGTGACAACATGACAAAGCT+CGG + chr1_1:70327537-70327556 None:intergenic 45.0%
TTGTCATGTTGTCACCTTGC+TGG - chr1_1:70327541-70327560 Msa0040250:intron 45.0%
TTGTCACCTTGCTGGCAAAT+AGG - chr1_1:70327549-70327568 Msa0040250:intron 45.0%
AGGCCAATCTCTCCATCATA+TGG + chr1_1:70327586-70327605 None:intergenic 45.0%
CAAAGGAGTGACAATCACAG+AGG - chr1_1:70327623-70327642 Msa0040250:intron 45.0%
TCCTGTACAGTAGACTCCAG+CGG + chr1_1:70326926-70326945 None:intergenic 50.0%
GGCAGCCAAAACCAAGATCT+AGG - chr1_1:70327043-70327062 Msa0040250:intron 50.0%
CTCCTAGTTACAGTGATGGC+AGG - chr1_1:70327224-70327243 Msa0040250:intron 50.0%
GATCGGAAGTGGTAGTTGCT+AGG - chr1_1:70327419-70327438 Msa0040250:intron 50.0%
CATCAGCCTATTTGCCAGCA+AGG + chr1_1:70327558-70327577 None:intergenic 50.0%
TTGTGCACATTGCCACAACG+AGG + chr1_1:70327606-70327625 None:intergenic 50.0%
CGTTGTGGCAATGTGCACAA+AGG - chr1_1:70327606-70327625 Msa0040250:intron 50.0%
AGGAGTGACAATCACAGAGG+TGG - chr1_1:70327626-70327645 Msa0040250:intron 50.0%
AGTGACAATCACAGAGGTGG+AGG - chr1_1:70327629-70327648 Msa0040250:intron 50.0%
AATCACAACCAGGTCGGTGT+TGG - chr1_1:70327706-70327725 Msa0040250:intron 50.0%
TGCATATTCCAACACCGACC+TGG + chr1_1:70327717-70327736 None:intergenic 50.0%
TCCTGTACAGTAGACTCCAG+CGG + chr1_1:70326926-70326945 None:intergenic 50.0%
GGCAGCCAAAACCAAGATCT+AGG - chr1_1:70327043-70327062 Msa0040250:intron 50.0%
CTCCTAGTTACAGTGATGGC+AGG - chr1_1:70327224-70327243 Msa0040250:intron 50.0%
GATCGGAAGTGGTAGTTGCT+AGG - chr1_1:70327419-70327438 Msa0040250:intron 50.0%
CATCAGCCTATTTGCCAGCA+AGG + chr1_1:70327558-70327577 None:intergenic 50.0%
TTGTGCACATTGCCACAACG+AGG + chr1_1:70327606-70327625 None:intergenic 50.0%
CGTTGTGGCAATGTGCACAA+AGG - chr1_1:70327606-70327625 Msa0040250:intron 50.0%
AGGAGTGACAATCACAGAGG+TGG - chr1_1:70327626-70327645 Msa0040250:intron 50.0%
AGTGACAATCACAGAGGTGG+AGG - chr1_1:70327629-70327648 Msa0040250:intron 50.0%
AATCACAACCAGGTCGGTGT+TGG - chr1_1:70327706-70327725 Msa0040250:intron 50.0%
TGCATATTCCAACACCGACC+TGG + chr1_1:70327717-70327736 None:intergenic 50.0%
TGTTGTGAGTGCTTTGCCGC+TGG - chr1_1:70326907-70326926 Msa0040250:CDS 55.0%
AGGCACTCCAACCGAGCATT+AGG - chr1_1:70327439-70327458 Msa0040250:intron 55.0%
!! TGGAGAGATTGGCCTCGTTG+TGG - chr1_1:70327591-70327610 Msa0040250:intron 55.0%
TGTTGTGAGTGCTTTGCCGC+TGG - chr1_1:70326907-70326926 Msa0040250:CDS 55.0%
AGGCACTCCAACCGAGCATT+AGG - chr1_1:70327439-70327458 Msa0040250:intron 55.0%
!! TGGAGAGATTGGCCTCGTTG+TGG - chr1_1:70327591-70327610 Msa0040250:intron 55.0%
GCCGCTGGAGTCTACTGTAC+AGG - chr1_1:70326922-70326941 Msa0040250:intron 60.0%
GCCGCTGGAGTCTACTGTAC+AGG - chr1_1:70326922-70326941 Msa0040250:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 70326783 70328003 70326783 ID=Msa0040250;Name=Msa0040250
chr1_1 mRNA 70326783 70328003 70326783 ID=Msa0040250-mRNA-1;Parent=Msa0040250;Name=Msa0040250-mRNA-1;_AED=0.47;_eAED=0.76;_QI=0|0|0|1|0|0|3|0|120
chr1_1 exon 70327962 70328003 70327962 ID=Msa0040250-mRNA-1:exon:23186;Parent=Msa0040250-mRNA-1
chr1_1 exon 70327761 70327924 70327761 ID=Msa0040250-mRNA-1:exon:23185;Parent=Msa0040250-mRNA-1
chr1_1 exon 70326783 70326939 70326783 ID=Msa0040250-mRNA-1:exon:23184;Parent=Msa0040250-mRNA-1
chr1_1 CDS 70327962 70328003 70327962 ID=Msa0040250-mRNA-1:cds;Parent=Msa0040250-mRNA-1
chr1_1 CDS 70327761 70327924 70327761 ID=Msa0040250-mRNA-1:cds;Parent=Msa0040250-mRNA-1
chr1_1 CDS 70326783 70326939 70326783 ID=Msa0040250-mRNA-1:cds;Parent=Msa0040250-mRNA-1
Gene Sequence

>Msa0040250

ATGCTTAGCGTGTTCTCTCTCAGTTCAATATTTTTGTTACATATTAACCTTCTTTTTTTACTCTTTTGCAACAATTTTATCCTCAGTTGTTGTGAGTGCTTTGCCGCTGGAGTCTACTGTACAGGAACTTGCTCATGTGAAGACTGCTTGAATAAACCTATTAATGAAGATTCAGTTCTGAAAGCTGGCAGGAATGAGCATTGTATAAGCATCACAGATAAAGCTACTAATTTGTATGTGACAGCAGACCATTGCTTCCATTACCTTATTCATCAATCGGAAAGCCGAAAAGATCTTGTGCTTAATTTTTTGGCATCCAAAACCAAAGAGCTTGTGGAATCAAATGCTATTGGACAAACGTGA

Protein sequence

>Msa0040250

MLSVFSLSSIFLLHINLLFLLFCNNFILSCCECFAAGVYCTGTCSCEDCLNKPINEDSVLKAGRNEHCISITDKATNLYVTADHCFHYLIHQSESRKDLVLNFLASKTKELVESNAIGQT*