AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0041920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0041920 MtrunA17_Chr4g0059701 88.462 52 6 0 36 87 79 130 7.86e-30 107
Msa0041920 MtrunA17_Chr8g0389241 67.143 70 18 1 20 89 89 153 2.47e-29 105
Msa0041920 MtrunA17_Chr6g0488471 81.481 54 10 0 36 89 92 145 4.72e-29 105
Msa0041920 MtrunA17_Chr5g0415191 81.132 53 10 0 36 88 97 149 2.01e-28 103
Msa0041920 MtrunA17_Chr2g0331031 74.074 54 14 0 36 89 104 157 6.95e-27 100
Msa0041920 MtrunA17_Chr4g0055931 75.926 54 13 0 36 89 90 143 9.12e-27 99.8
Msa0041920 MtrunA17_Chr6g0452791 87.500 48 6 0 41 88 80 127 1.46e-26 95.9
Msa0041920 MtrunA17_Chr3g0092621 80.769 52 10 0 37 88 80 131 1.90e-26 95.5
Msa0041920 MtrunA17_Chr3g0104071 75.926 54 13 0 36 89 142 195 2.08e-26 98.6
Msa0041920 MtrunA17_Chr5g0429521 75.926 54 13 0 36 89 127 180 2.61e-26 98.2
Msa0041920 MtrunA17_Chr7g0241761 74.074 54 14 0 36 89 135 188 5.03e-26 97.4
Msa0041920 MtrunA17_Chr4g0062741 74.074 54 14 0 36 89 133 186 5.62e-26 97.4
Msa0041920 MtrunA17_Chr1g0162081 74.074 54 14 0 36 89 117 170 3.18e-25 95.1
Msa0041920 MtrunA17_Chr1g0210471 66.154 65 22 0 25 89 108 172 1.98e-24 92.0
Msa0041920 MtrunA17_Chr5g0436501 70.370 54 16 0 36 89 120 173 5.73e-24 91.3
Msa0041920 MtrunA17_Chr3g0135671 76.000 50 12 0 40 89 90 139 1.34e-23 89.7
Msa0041920 MtrunA17_Chr5g0440731 62.500 48 18 0 41 88 105 152 1.43e-17 74.7
Msa0041920 MtrunA17_Chr5g0440741 65.217 46 16 0 43 88 102 147 1.55e-17 74.7
Msa0041920 MtrunA17_Chr5g0409831 62.500 48 18 0 41 88 77 124 3.86e-17 72.8
Msa0041920 MtrunA17_Chr5g0409841 63.043 46 17 0 43 88 94 139 4.96e-16 69.7
Msa0041920 MtrunA17_Chr8g0365011 52.632 57 15 1 43 87 94 150 1.73e-14 66.2
Msa0041920 MtrunA17_Chr5g0432921 63.043 46 16 1 43 88 115 159 5.90e-13 61.6
Msa0041920 MtrunA17_Chr5g0432941 50.820 61 29 1 28 88 100 159 7.19e-13 61.2
Msa0041920 MtrunA17_Chr1g0192061 47.619 63 30 2 27 89 49 108 9.39e-13 60.5
Msa0041920 MtrunA17_Chr8g0364731 70.000 40 12 0 50 89 10 49 5.96e-12 58.2
Msa0041920 MtrunA17_Chr3g0113121 55.319 47 21 0 43 89 97 143 8.98e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0041920 AT5G47390.1 79.630 54 11 0 36 89 91 144 5.41e-28 103
Msa0041920 AT1G70000.1 81.132 53 10 0 36 88 93 145 4.43e-27 99.8
Msa0041920 AT1G70000.2 81.132 53 10 0 36 88 93 145 4.43e-27 99.8
Msa0041920 AT3G16350.1 77.358 53 12 0 36 88 132 184 6.96e-27 101
Msa0041920 AT5G58900.1 75.926 54 13 0 36 89 136 189 1.73e-26 98.6
Msa0041920 AT1G19000.2 81.250 48 9 0 40 87 101 148 3.64e-26 97.8
Msa0041920 AT1G19000.1 81.250 48 9 0 40 87 101 148 3.64e-26 97.8
Msa0041920 AT2G38090.1 72.222 54 15 0 36 89 136 189 1.60e-25 96.3
Msa0041920 AT1G74840.2 81.250 48 9 0 40 87 95 142 2.11e-25 94.7
Msa0041920 AT1G74840.1 81.250 48 9 0 40 87 95 142 2.64e-25 95.1
Msa0041920 AT1G49010.1 74.074 54 14 0 36 89 129 182 3.13e-25 95.9
Msa0041920 AT5G56840.1 78.000 50 11 0 40 89 90 139 3.86e-25 94.0
Msa0041920 AT5G08520.1 72.222 54 15 0 36 89 113 166 1.52e-24 93.6
Msa0041920 AT5G61620.1 70.370 54 16 0 36 89 103 156 2.17e-24 93.6
Msa0041920 AT5G04760.1 72.727 55 15 0 35 89 93 147 5.48e-24 90.5
Msa0041920 AT5G05790.2 72.000 50 14 0 40 89 130 179 6.89e-23 89.0
Msa0041920 AT5G05790.1 72.000 50 14 0 40 89 130 179 6.89e-23 89.0
Msa0041920 AT5G01200.1 68.519 54 17 0 36 89 141 194 6.73e-22 86.3
Msa0041920 AT3G11280.2 72.000 50 14 0 40 89 126 175 1.07e-21 85.5
Msa0041920 AT3G11280.1 72.000 50 14 0 40 89 126 175 1.07e-21 85.5
Msa0041920 AT3G10580.1 68.000 50 16 0 40 89 94 143 1.85e-20 82.8
Msa0041920 AT3G10580.2 68.000 50 16 0 40 89 94 143 1.30e-19 80.1
Msa0041920 AT4G09450.1 68.627 51 16 0 39 89 89 139 1.44e-19 79.0
Msa0041920 AT5G23650.1 68.750 48 15 0 40 87 119 166 1.03e-17 75.9
Msa0041920 AT3G10590.1 41.667 84 47 1 5 86 73 156 1.69e-12 60.5

Find 10 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTTGGATTGGAGAAAGT+GGG 0.463944 1_1:-72504723 Msa0041920:CDS
AGAAAGTGGGAAAAGGTGAT+TGG 0.482958 1_1:-72504710 Msa0041920:CDS
TTTGGTTGGATTGGAGAAAG+TGG 0.531455 1_1:-72504724 Msa0041920:CDS
GGATTGGAGAAAGTGGGAAA+AGG 0.562648 1_1:-72504717 Msa0041920:CDS
TTGTGCTCTTCCTCAGTCCA+CGG 0.578432 1_1:+72504753 None:intergenic
GTGATTGGAGAGGAATCTCA+AGG 0.619264 1_1:-72504695 Msa0041920:CDS
GTGGGAAAAGGTGATTGGAG+AGG 0.624127 1_1:-72504705 Msa0041920:CDS
TGAATGGATCAGGGATTCCG+TGG 0.626840 1_1:-72504770 Msa0041920:intron
GCATGACTTGCAACATGCGT+AGG 0.636946 1_1:+72504651 None:intergenic
ATCAGGGATTCCGTGGACTG+AGG 0.660333 1_1:-72504763 Msa0041920:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAATAATAAAAATTGGA+TGG + chr1_1:72504727-72504746 None:intergenic 15.0%
!! CTTGCAAAAATAATAAAAAT+TGG + chr1_1:72504731-72504750 None:intergenic 15.0%
!! CAAAAATAATAAAAATTGGA+TGG + chr1_1:72504727-72504746 None:intergenic 15.0%
!! CTTGCAAAAATAATAAAAAT+TGG + chr1_1:72504731-72504750 None:intergenic 15.0%
!! TATTGAAGCAAAAATATTCA+TGG + chr1_1:72504671-72504690 None:intergenic 20.0%
!! ATTTCAATCAATTCAAACAA+AGG + chr1_1:72505235-72505254 None:intergenic 20.0%
!!! TGTTATTGATTTTCTTTGAA+TGG - chr1_1:72505269-72505288 Msa0041920:CDS 20.0%
!! TATTGAAGCAAAAATATTCA+TGG + chr1_1:72504671-72504690 None:intergenic 20.0%
!! ATTTCAATCAATTCAAACAA+AGG + chr1_1:72505235-72505254 None:intergenic 20.0%
!!! TGTTATTGATTTTCTTTGAA+TGG - chr1_1:72505269-72505288 Msa0041920:CDS 20.0%
! CTTCAATATTAATTGTGTCT+TGG - chr1_1:72504683-72504702 Msa0041920:CDS 25.0%
! AATATCTACTTAAAATCCTG+AGG + chr1_1:72505084-72505103 None:intergenic 25.0%
!! TCTCACATTTAAATGGTTTT+TGG - chr1_1:72505140-72505159 Msa0041920:intron 25.0%
! CTTCAATATTAATTGTGTCT+TGG - chr1_1:72504683-72504702 Msa0041920:CDS 25.0%
! AATATCTACTTAAAATCCTG+AGG + chr1_1:72505084-72505103 None:intergenic 25.0%
!! TCTCACATTTAAATGGTTTT+TGG - chr1_1:72505140-72505159 Msa0041920:intron 25.0%
TTTAATCTTTCATCTCTAGC+AGG - chr1_1:72504765-72504784 Msa0041920:intron 30.0%
!!! ACTCAGCAACAAACATTTTT+TGG + chr1_1:72504816-72504835 None:intergenic 30.0%
CAAATTCACAAAAAACACAC+AGG + chr1_1:72504870-72504889 None:intergenic 30.0%
!!! GTTTTTTGTGAATTTGCTTC+TGG - chr1_1:72504874-72504893 Msa0041920:intron 30.0%
AATGCAGCACAATTCAAAAT+TGG + chr1_1:72504936-72504955 None:intergenic 30.0%
ACATGTGCACAAACTTTAAT+TGG - chr1_1:72504999-72505018 Msa0041920:intron 30.0%
ATAACTACATCTTATGCAAG+AGG - chr1_1:72505023-72505042 Msa0041920:intron 30.0%
CTACATGTCTCACATTTAAA+TGG - chr1_1:72505133-72505152 Msa0041920:intron 30.0%
! TGATTTTCTTTGAATGGATC+AGG - chr1_1:72505275-72505294 Msa0041920:intron 30.0%
! GATTTTCTTTGAATGGATCA+GGG - chr1_1:72505276-72505295 Msa0041920:intron 30.0%
TTTAATCTTTCATCTCTAGC+AGG - chr1_1:72504765-72504784 Msa0041920:intron 30.0%
!!! ACTCAGCAACAAACATTTTT+TGG + chr1_1:72504816-72504835 None:intergenic 30.0%
CAAATTCACAAAAAACACAC+AGG + chr1_1:72504870-72504889 None:intergenic 30.0%
!!! GTTTTTTGTGAATTTGCTTC+TGG - chr1_1:72504874-72504893 Msa0041920:intron 30.0%
AATGCAGCACAATTCAAAAT+TGG + chr1_1:72504936-72504955 None:intergenic 30.0%
ACATGTGCACAAACTTTAAT+TGG - chr1_1:72504999-72505018 Msa0041920:intron 30.0%
ATAACTACATCTTATGCAAG+AGG - chr1_1:72505023-72505042 Msa0041920:intron 30.0%
CTACATGTCTCACATTTAAA+TGG - chr1_1:72505133-72505152 Msa0041920:intron 30.0%
! TGATTTTCTTTGAATGGATC+AGG - chr1_1:72505275-72505294 Msa0041920:intron 30.0%
! GATTTTCTTTGAATGGATCA+GGG - chr1_1:72505276-72505295 Msa0041920:intron 30.0%
GATGGAGCAAATTGAAACAA+AGG + chr1_1:72504709-72504728 None:intergenic 35.0%
ACAAAAAACACACAGGATAG+GGG + chr1_1:72504863-72504882 None:intergenic 35.0%
CACAAAAAACACACAGGATA+GGG + chr1_1:72504864-72504883 None:intergenic 35.0%
TCACAAAAAACACACAGGAT+AGG + chr1_1:72504865-72504884 None:intergenic 35.0%
!! GAATTTGCTTCTGGTTTGTA+AGG - chr1_1:72504883-72504902 Msa0041920:intron 35.0%
!! TAGCAGGTTTGTTACTTACA+AGG - chr1_1:72504906-72504925 Msa0041920:intron 35.0%
TTGTGCTGCATTATTCATCT+AGG - chr1_1:72504944-72504963 Msa0041920:intron 35.0%
TGTGCTGCATTATTCATCTA+GGG - chr1_1:72504945-72504964 Msa0041920:intron 35.0%
TCACTTGGATTGAGCATTAA+AGG - chr1_1:72505051-72505070 Msa0041920:intron 35.0%
CATTAAAGGTTGTTTACCTC+AGG - chr1_1:72505065-72505084 Msa0041920:intron 35.0%
!!! CAAGATGTTTTTGGTTGGAT+TGG - chr1_1:72505322-72505341 Msa0041920:intron 35.0%
GATGGAGCAAATTGAAACAA+AGG + chr1_1:72504709-72504728 None:intergenic 35.0%
ACAAAAAACACACAGGATAG+GGG + chr1_1:72504863-72504882 None:intergenic 35.0%
CACAAAAAACACACAGGATA+GGG + chr1_1:72504864-72504883 None:intergenic 35.0%
TCACAAAAAACACACAGGAT+AGG + chr1_1:72504865-72504884 None:intergenic 35.0%
!! GAATTTGCTTCTGGTTTGTA+AGG - chr1_1:72504883-72504902 Msa0041920:intron 35.0%
!! TAGCAGGTTTGTTACTTACA+AGG - chr1_1:72504906-72504925 Msa0041920:intron 35.0%
TTGTGCTGCATTATTCATCT+AGG - chr1_1:72504944-72504963 Msa0041920:intron 35.0%
TGTGCTGCATTATTCATCTA+GGG - chr1_1:72504945-72504964 Msa0041920:intron 35.0%
TCACTTGGATTGAGCATTAA+AGG - chr1_1:72505051-72505070 Msa0041920:intron 35.0%
CATTAAAGGTTGTTTACCTC+AGG - chr1_1:72505065-72505084 Msa0041920:intron 35.0%
!!! CAAGATGTTTTTGGTTGGAT+TGG - chr1_1:72505322-72505341 Msa0041920:intron 35.0%
CAAAAAACACACAGGATAGG+GGG + chr1_1:72504862-72504881 None:intergenic 40.0%
GTGCTGCATTATTCATCTAG+GGG - chr1_1:72504946-72504965 Msa0041920:intron 40.0%
ATGCAAGAGGAACTTTCACT+TGG - chr1_1:72505036-72505055 Msa0041920:intron 40.0%
CATTCTCTCTGAGCAAACAA+TGG - chr1_1:72505110-72505129 Msa0041920:intron 40.0%
!! GGAAGAGCACAAGATGTTTT+TGG - chr1_1:72505313-72505332 Msa0041920:intron 40.0%
!!! GAGCACAAGATGTTTTTGGT+TGG - chr1_1:72505317-72505336 Msa0041920:intron 40.0%
! TTTGGTTGGATTGGAGAAAG+TGG - chr1_1:72505331-72505350 Msa0041920:intron 40.0%
! TTGGTTGGATTGGAGAAAGT+GGG - chr1_1:72505332-72505351 Msa0041920:intron 40.0%
AGAAAGTGGGAAAAGGTGAT+TGG - chr1_1:72505345-72505364 Msa0041920:intron 40.0%
CAAAAAACACACAGGATAGG+GGG + chr1_1:72504862-72504881 None:intergenic 40.0%
GTGCTGCATTATTCATCTAG+GGG - chr1_1:72504946-72504965 Msa0041920:intron 40.0%
ATGCAAGAGGAACTTTCACT+TGG - chr1_1:72505036-72505055 Msa0041920:intron 40.0%
CATTCTCTCTGAGCAAACAA+TGG - chr1_1:72505110-72505129 Msa0041920:intron 40.0%
!! GGAAGAGCACAAGATGTTTT+TGG - chr1_1:72505313-72505332 Msa0041920:intron 40.0%
!!! GAGCACAAGATGTTTTTGGT+TGG - chr1_1:72505317-72505336 Msa0041920:intron 40.0%
! TTTGGTTGGATTGGAGAAAG+TGG - chr1_1:72505331-72505350 Msa0041920:intron 40.0%
! TTGGTTGGATTGGAGAAAGT+GGG - chr1_1:72505332-72505351 Msa0041920:intron 40.0%
AGAAAGTGGGAAAAGGTGAT+TGG - chr1_1:72505345-72505364 Msa0041920:intron 40.0%
!! CTTCTGGTTTGTAAGGTAGC+AGG - chr1_1:72504890-72504909 Msa0041920:intron 45.0%
GGATTGGAGAAAGTGGGAAA+AGG - chr1_1:72505338-72505357 Msa0041920:intron 45.0%
GTGATTGGAGAGGAATCTCA+AGG - chr1_1:72505360-72505379 Msa0041920:intron 45.0%
!! CTTCTGGTTTGTAAGGTAGC+AGG - chr1_1:72504890-72504909 Msa0041920:intron 45.0%
GGATTGGAGAAAGTGGGAAA+AGG - chr1_1:72505338-72505357 Msa0041920:intron 45.0%
GTGATTGGAGAGGAATCTCA+AGG - chr1_1:72505360-72505379 Msa0041920:intron 45.0%
GGCTGAGAAGAGACTGCTAT+TGG - chr1_1:72504967-72504986 Msa0041920:intron 50.0%
TGAATGGATCAGGGATTCCG+TGG - chr1_1:72505285-72505304 Msa0041920:intron 50.0%
TTGTGCTCTTCCTCAGTCCA+CGG + chr1_1:72505305-72505324 None:intergenic 50.0%
GTGGGAAAAGGTGATTGGAG+AGG - chr1_1:72505350-72505369 Msa0041920:intron 50.0%
! GCATGACTTGCAACATGCGT+AGG + chr1_1:72505407-72505426 None:intergenic 50.0%
GGCTGAGAAGAGACTGCTAT+TGG - chr1_1:72504967-72504986 Msa0041920:intron 50.0%
TGAATGGATCAGGGATTCCG+TGG - chr1_1:72505285-72505304 Msa0041920:intron 50.0%
TTGTGCTCTTCCTCAGTCCA+CGG + chr1_1:72505305-72505324 None:intergenic 50.0%
GTGGGAAAAGGTGATTGGAG+AGG - chr1_1:72505350-72505369 Msa0041920:intron 50.0%
! GCATGACTTGCAACATGCGT+AGG + chr1_1:72505407-72505426 None:intergenic 50.0%
! ATCAGGGATTCCGTGGACTG+AGG - chr1_1:72505292-72505311 Msa0041920:intron 55.0%
! ATCAGGGATTCCGTGGACTG+AGG - chr1_1:72505292-72505311 Msa0041920:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 72504629 72505448 72504629 ID=Msa0041920;Name=Msa0041920
chr1_1 mRNA 72504629 72505448 72504629 ID=Msa0041920-mRNA-1;Parent=Msa0041920;Name=Msa0041920-mRNA-1;_AED=0.21;_eAED=0.20;_QI=0|0|0|0.66|0.5|0.66|3|0|89
chr1_1 exon 72505440 72505448 72505440 ID=Msa0041920-mRNA-1:exon:24168;Parent=Msa0041920-mRNA-1
chr1_1 exon 72505182 72505290 72505182 ID=Msa0041920-mRNA-1:exon:24167;Parent=Msa0041920-mRNA-1
chr1_1 exon 72504629 72504780 72504629 ID=Msa0041920-mRNA-1:exon:24166;Parent=Msa0041920-mRNA-1
chr1_1 CDS 72505440 72505448 72505440 ID=Msa0041920-mRNA-1:cds;Parent=Msa0041920-mRNA-1
chr1_1 CDS 72505182 72505290 72505182 ID=Msa0041920-mRNA-1:cds;Parent=Msa0041920-mRNA-1
chr1_1 CDS 72504629 72504780 72504629 ID=Msa0041920-mRNA-1:cds;Parent=Msa0041920-mRNA-1
Gene Sequence

>Msa0041920

ATGGATAGAGCAATGTCTATCATGAAGATTCAATACCAAAAAATGTTTGTTGCTGAGTTTTTGAGAGCTGTTTTTTGTGTGCCCCCTATCCTGTGTGTTTTTTGTGAATTTGCTTCTGGGATTCCGTGGACTGAGGAAGAGCACAAGATGTTTTTGGTTGGATTGGAGAAAGTGGGAAAAGGTGATTGGAGAGGAATCTCAAGGAACTATGTGAAAACAAGAACGCCTACGCATGTTGCAAGTCATGCTCAGAAGTACTTTCTCCGCTGA

Protein sequence

>Msa0041920

MDRAMSIMKIQYQKMFVAEFLRAVFCVPPILCVFCEFASGIPWTEEEHKMFLVGLEKVGKGDWRGISRNYVKTRTPTHVASHAQKYFLR*