AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0042450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0042450 MtrunA17_Chr1g0191011 90.831 349 30 2 130 477 28 375 0.0 605
Msa0042450 MtrunA17_Chr1g0191021 51.630 184 28 2 1 123 86 269 8.43e-42 153
Msa0042450 MtrunA17_Chr1g0191021 32.295 353 186 12 160 473 32 370 1.38e-40 149
Msa0042450 MtrunA17_Chr7g0273821 44.444 171 76 7 174 331 42 206 5.03e-28 115
Msa0042450 MtrunA17_Chr7g0273821 68.627 51 16 0 7 57 106 156 1.76e-16 81.6
Msa0042450 MtrunA17_Chr7g0273821 37.607 117 50 5 378 475 323 435 8.43e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0042450 AT5G28040.2 33.425 365 160 13 179 474 72 422 2.05e-43 159
Msa0042450 AT5G28040.1 33.425 365 160 13 179 474 72 422 2.05e-43 159
Msa0042450 AT3G04930.2 38.012 171 86 6 179 337 78 240 9.33e-27 112
Msa0042450 AT3G04930.2 59.259 54 20 2 6 57 132 185 2.25e-11 65.9
Msa0042450 AT3G04930.1 38.012 171 86 6 179 337 78 240 1.99e-26 112
Msa0042450 AT3G04930.1 59.259 54 20 2 6 57 132 185 2.62e-11 65.9
Msa0042450 AT1G11510.1 39.394 99 53 2 234 331 138 230 1.10e-12 69.7
Msa0042450 AT1G11510.2 39.394 99 53 2 234 331 109 201 1.13e-12 69.3
Msa0042450 AT1G61730.1 31.206 141 88 5 190 330 114 245 2.06e-11 65.9

Find 106 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAATTCCACGCTGAATT+TGG 0.141013 1_1:-73313122 None:intergenic
TCAGTCAAATGAGCTGAATT+AGG 0.184630 1_1:-73310835 None:intergenic
TCGAAATTTCCCACAAGATT+TGG 0.203495 1_1:+73313257 Msa0042450:CDS
CGAAATTTCCCACAAGATTT+GGG 0.213735 1_1:+73313258 Msa0042450:CDS
CTGAATTAGGGTTAGGGTTT+GGG 0.227113 1_1:-73310822 None:intergenic
TCTTCTTATCATAATGATAT+TGG 0.242509 1_1:+73310556 Msa0042450:CDS
CTTGTGGGAAATTTCGAAAA+TGG 0.256771 1_1:-73313251 None:intergenic
AGAAGTTACGTAGGTTAAAA+AGG 0.283260 1_1:+73313161 Msa0042450:CDS
TCAATAAGATCAGGACTTTC+AGG 0.284560 1_1:-73313328 None:intergenic
AAGATTACTGTTACTCTTAT+CGG 0.285919 1_1:-73310986 None:intergenic
ACCATTGTTGAAGGAACTAT+TGG 0.296222 1_1:+73313525 Msa0042450:CDS
TGAACTGTTGAAGGGATATC+TGG 0.305389 1_1:+73313024 Msa0042450:CDS
ATTTCCTCATATTCTTCAAT+AGG 0.320555 1_1:-73310796 None:intergenic
CAATGACACCGACCACGTTT+TGG 0.330065 1_1:+73313282 Msa0042450:CDS
ATGGTTAAAACGGAGGATTT+TGG 0.338805 1_1:+73313618 Msa0042450:CDS
CAGTCAAATGAGCTGAATTA+GGG 0.341975 1_1:-73310834 None:intergenic
GCTGAATTAGGGTTAGGGTT+TGG 0.352389 1_1:-73310823 None:intergenic
AAATGAGCTGAATTAGGGTT+AGG 0.355729 1_1:-73310829 None:intergenic
TGGTCTTCATCCACATTGTA+TGG 0.361996 1_1:-73312785 None:intergenic
TTGAATTCCACGCTGAATTT+GGG 0.366580 1_1:-73313121 None:intergenic
GCCATGGCCACCGTCACAAC+GGG 0.369780 1_1:-73312851 None:intergenic
GACGCACTGGGTGGCTCTAC+TGG 0.372415 1_1:-73313556 None:intergenic
ATTGTGAAGAAACAGATAAA+AGG 0.382017 1_1:+73313383 Msa0042450:CDS
CATTGTATGGACATAGAAAA+TGG 0.389529 1_1:-73312772 None:intergenic
TGGTGAGAAACAAGACTTCA+TGG 0.394662 1_1:-73313506 None:intergenic
AACAATAACTCAACTCCAAT+TGG 0.394865 1_1:+73310769 Msa0042450:CDS
CATCATCCACATTGTCCATT+TGG 0.402335 1_1:-73312739 None:intergenic
GCCGTGTTTGTGTCATCTGC+GGG 0.417687 1_1:-73312878 None:intergenic
ATCGTTCTATGATCAAATTA+AGG 0.428455 1_1:+73310579 Msa0042450:CDS
TAGTAGCATTGCCATTGTTC+CGG 0.431642 1_1:-73313459 None:intergenic
TGAGGGATCTTGAAAGAAAT+CGG 0.433564 1_1:-73313220 None:intergenic
TTGTTTCTCACCATTGTTGA+AGG 0.439029 1_1:+73313516 Msa0042450:CDS
TAACAACATCGGCGACGCAC+TGG 0.453222 1_1:-73313569 None:intergenic
AGATCAGGACTTTCAGGAAC+AGG 0.454735 1_1:-73313322 None:intergenic
CAATGGGAATGTTGTCCATT+TGG 0.457011 1_1:-73312805 None:intergenic
CCCCGTTGTGACGGTGGCCA+TGG 0.457760 1_1:+73312850 Msa0042450:CDS
TCTTCAATAGGAACACCAAT+TGG 0.467577 1_1:-73310784 None:intergenic
TGACAATGTTGAAGACCAAA+TGG 0.469140 1_1:+73312724 Msa0042450:CDS
AATTCAGCGTGGAATTCAAC+AGG 0.472407 1_1:+73313125 Msa0042450:CDS
AATGAGCTGAATTAGGGTTA+GGG 0.478935 1_1:-73310828 None:intergenic
TGTTCCTATTGAAGAATATG+AGG 0.484128 1_1:+73310792 Msa0042450:CDS
TCAGAAACGGCAAGTGCAAT+GGG 0.486716 1_1:-73312821 None:intergenic
GGACGAGATTGAACTGTTGA+AGG 0.488252 1_1:+73313015 Msa0042450:CDS
CTTCTTTAGTCCAGAGACAT+TGG 0.490028 1_1:-73310483 None:intergenic
TATTGACAACGTTAAGATGA+AGG 0.490932 1_1:+73313345 Msa0042450:CDS
AAGGAAACACAAGATGACTT+TGG 0.497734 1_1:+73313180 Msa0042450:CDS
CAATGTGGATGAAGACCAAA+TGG 0.497943 1_1:+73312790 Msa0042450:CDS
CTCGATTACAACGCCAATCG+TGG 0.500789 1_1:+73310532 Msa0042450:CDS
ATCAGAAACGGCAAGTGCAA+TGG 0.502613 1_1:-73312822 None:intergenic
TTAAACAATATCAGAGGCTA+TGG 0.508336 1_1:+73312984 Msa0042450:CDS
TCAGATCAAGGCTAGGTTGG+AGG 0.516669 1_1:+73313681 Msa0042450:CDS
TTGTGAAGAAACAGATAAAA+GGG 0.517946 1_1:+73313384 Msa0042450:CDS
TCGAAAATGGCTTGTTCCTG+AGG 0.518443 1_1:-73313238 None:intergenic
TTAACGTTGTCAATAAGATC+AGG 0.519347 1_1:-73313337 None:intergenic
GGAGGTGTATTTGAATCGGT+TGG 0.520311 1_1:+73313645 Msa0042450:CDS
GTCATTGCCCCAAATCTTGT+GGG 0.523632 1_1:-73313266 None:intergenic
AACTGGAGGTGTATTTGAAT+CGG 0.526700 1_1:+73313641 Msa0042450:CDS
CGAAAATGGCTTGTTCCTGA+GGG 0.533678 1_1:-73313237 None:intergenic
GATTTCTTTCAAGATCCCTC+AGG 0.542535 1_1:+73313222 Msa0042450:CDS
TGTCATTGCCCCAAATCTTG+TGG 0.546121 1_1:-73313267 None:intergenic
AATCGGTTGGAGTTGTTGCA+AGG 0.547050 1_1:+73313658 Msa0042450:CDS
GGCAACAAACACAGCCACCA+CGG 0.547339 1_1:+73312898 Msa0042450:CDS
GACGAGATTACGATCTTGCA+AGG 0.547606 1_1:+73310505 Msa0042450:CDS
CGCCGATGTTGTTATCGACA+AGG 0.549485 1_1:+73313578 Msa0042450:CDS
AACAACATCGGCGACGCACT+GGG 0.551267 1_1:-73313568 None:intergenic
TCCAATAGTTCCTTCAACAA+TGG 0.555130 1_1:-73313526 None:intergenic
ATGGCAATGCTACTAGCATA+CGG 0.556871 1_1:+73313467 Msa0042450:CDS
TGGCAATGCTACTAGCATAC+GGG 0.563532 1_1:+73313468 Msa0042450:CDS
GGGATGAACAATGGTTAAAA+CGG 0.566430 1_1:+73313608 Msa0042450:CDS
CTGGATTACATTAAACAGCA+TGG 0.577356 1_1:+73313043 Msa0042450:CDS
TGCCGTGTTTGTGTCATCTG+CGG 0.578987 1_1:-73312879 None:intergenic
AGCCATGGCCACCGTCACAA+CGG 0.580091 1_1:-73312852 None:intergenic
CAATGTGGATGATGACGAAA+AGG 0.583654 1_1:+73312748 Msa0042450:CDS
GACTTTGGACAAAGGCAAAG+AGG 0.584181 1_1:+73313195 Msa0042450:CDS
TACGTAACTTCTCAACAAGC+TGG 0.586441 1_1:-73313150 None:intergenic
GTTGTTGCAAGGTCAGATCA+AGG 0.586461 1_1:+73313669 Msa0042450:CDS
TCAGAGGCTATGGACAAAGC+AGG 0.591463 1_1:+73312994 Msa0042450:CDS
AGGTCAGATCAAGGCTAGGT+TGG 0.595411 1_1:+73313678 Msa0042450:CDS
ATGATAAGAAGATCCACGAT+TGG 0.603760 1_1:-73310545 None:intergenic
GAAATTTCCCACAAGATTTG+GGG 0.610345 1_1:+73313259 Msa0042450:CDS
TGTTATCGACAAGGGAAGTG+AGG 0.611514 1_1:+73313587 Msa0042450:CDS
CCCCGCAGATGACACAAACA+CGG 0.617705 1_1:+73312877 Msa0042450:CDS
GTTATCGACAAGGGAAGTGA+GGG 0.619048 1_1:+73313588 Msa0042450:CDS
GACGAGATTGAACTGTTGAA+GGG 0.620700 1_1:+73313016 Msa0042450:CDS
CACAAGATGACTTTGGACAA+AGG 0.621465 1_1:+73313187 Msa0042450:CDS
CGTGTTTGTGTCATCTGCGG+GGG 0.627292 1_1:-73312876 None:intergenic
GCCGATGTTGTTATCGACAA+GGG 0.629409 1_1:+73313579 Msa0042450:CDS
ATCAACTTCCAAAACGTGGT+CGG 0.637946 1_1:-73313290 None:intergenic
CCGTGTTTGTGTCATCTGCG+GGG 0.639190 1_1:-73312877 None:intergenic
TGCAAGGTCAGATCAAGGCT+AGG 0.648837 1_1:+73313674 Msa0042450:CDS
GGCAATGCTACTAGCATACG+GGG 0.651150 1_1:+73313469 Msa0042450:CDS
GGGAAGTGAGGGATGAACAA+TGG 0.665323 1_1:+73313599 Msa0042450:CDS
ATGAACAATGGTTAAAACGG+AGG 0.670080 1_1:+73313611 Msa0042450:CDS
CATGGCCACCGTCACAACGG+GGG 0.676320 1_1:-73312849 None:intergenic
CCATGGCCACCGTCACAACG+GGG 0.678792 1_1:-73312850 None:intergenic
TCCCTTGTCGATAACAACAT+CGG 0.682290 1_1:-73313580 None:intergenic
ACGGCGTTAAACAATATCAG+AGG 0.682928 1_1:+73312978 Msa0042450:CDS
GCAAATACTACTATTCAAAG+CGG 0.683552 1_1:+73313070 Msa0042450:CDS
ATTCATCAACTTCCAAAACG+TGG 0.687883 1_1:-73313294 None:intergenic
AGTTATTCCCAAATTCAGCG+TGG 0.688982 1_1:+73313114 Msa0042450:CDS
AGTGGTAGTAGCCACCGTGG+TGG 0.692224 1_1:-73312912 None:intergenic
TGAAGACCAAATGGACAATG+TGG 0.693003 1_1:+73312733 Msa0042450:CDS
AACATCGGCGACGCACTGGG+TGG 0.698538 1_1:-73313565 None:intergenic
AGCTTGTTGAGAAGTTACGT+AGG 0.729557 1_1:+73313152 Msa0042450:CDS
AACAAACACAGCCACCACGG+TGG 0.758033 1_1:+73312901 Msa0042450:CDS
TATAGTGGTAGTAGCCACCG+TGG 0.762058 1_1:-73312915 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAATATTTTTATAACTT+TGG + chr1_1:73311556-73311575 Msa0042450:intron 10.0%
!!! AAAAAGAAATAGTTTTTTTT+AGG + chr1_1:73311601-73311620 Msa0042450:intron 10.0%
!!! AAAAGAAATAGTTTTTTTTA+GGG + chr1_1:73311602-73311621 Msa0042450:intron 10.0%
!! TTAGAATTAGATTTATATAT+TGG + chr1_1:73311627-73311646 Msa0042450:intron 10.0%
!!! TAGTTTTTTTTTTTTTTTGA+AGG + chr1_1:73311652-73311671 Msa0042450:intron 10.0%
!! ATAATTAAAATCTAAACAAA+TGG + chr1_1:73311923-73311942 Msa0042450:intron 10.0%
!! ATATTTATACTATGTATAAA+AGG + chr1_1:73311955-73311974 Msa0042450:intron 10.0%
!! ATACAATAATGTTATATTAT+TGG + chr1_1:73312099-73312118 Msa0042450:intron 10.0%
!! ATACAATAATGTTATATTAT+TGG + chr1_1:73312099-73312118 Msa0042450:intron 10.0%
!! AAATTCAGTTAAATATATAA+AGG - chr1_1:73312425-73312444 None:intergenic 10.0%
!!! TTAACTGAATTTAAAATTTA+CGG + chr1_1:73312433-73312452 Msa0042450:intron 10.0%
!! TGAATAGAAATAATTATAAA+TGG - chr1_1:73312491-73312510 None:intergenic 10.0%
!! TTAATCAATATACAAAAATC+AGG - chr1_1:73311231-73311250 None:intergenic 15.0%
!!! AAAGAAATAGTTTTTTTTAG+GGG + chr1_1:73311603-73311622 Msa0042450:intron 15.0%
!!! TTTTTTTGAAGGAAATATAT+TGG + chr1_1:73311663-73311682 Msa0042450:intron 15.0%
!! GAAAAAGAGTAAAAAAAATT+TGG - chr1_1:73311729-73311748 None:intergenic 15.0%
!! AATTTATATTTATACTCTTC+AGG + chr1_1:73312140-73312159 Msa0042450:intron 15.0%
!! ATTTATATTTATACTCTTCA+GGG + chr1_1:73312141-73312160 Msa0042450:intron 15.0%
!! AATTTATATTTATACTCTTC+AGG + chr1_1:73312140-73312159 Msa0042450:intron 15.0%
!! ATTTATATTTATACTCTTCA+GGG + chr1_1:73312141-73312160 Msa0042450:intron 15.0%
!! GTTAAATATATAAAGGATAA+AGG - chr1_1:73312418-73312437 None:intergenic 15.0%
!! TCTTCTTATCATAATGATAT+TGG + chr1_1:73310556-73310575 Msa0042450:CDS 20.0%
!! GAAATCAAATTTCTTAAGAA+CGG - chr1_1:73310677-73310696 None:intergenic 20.0%
!!! GTTCTTAAGAAATTTGATTT+CGG + chr1_1:73310676-73310695 Msa0042450:intron 20.0%
!!! AGTAGATTATTTTGATATGT+AGG - chr1_1:73311036-73311055 None:intergenic 20.0%
!! AAATAATCTACTGTCATATT+TGG + chr1_1:73311044-73311063 Msa0042450:intron 20.0%
!! CTTTGCAACAAATAAAAAAA+AGG + chr1_1:73311267-73311286 Msa0042450:intron 20.0%
!! TTTATACTATGTATAAAAGG+TGG + chr1_1:73311958-73311977 Msa0042450:intron 20.0%
!!! TATAACAAAAAGTATTCAGA+CGG + chr1_1:73312959-73312978 Msa0042450:CDS 20.0%
! ATCGTTCTATGATCAAATTA+AGG + chr1_1:73310579-73310598 Msa0042450:CDS 25.0%
!! TAAGAAATTTGATTTCGGTA+AGG + chr1_1:73310681-73310700 Msa0042450:intron 25.0%
!! GATTTTGTGTGAAATTTCAA+AGG - chr1_1:73310740-73310759 None:intergenic 25.0%
! ATTTCCTCATATTCTTCAAT+AGG - chr1_1:73310799-73310818 None:intergenic 25.0%
! AAGATTACTGTTACTCTTAT+CGG - chr1_1:73310989-73311008 None:intergenic 25.0%
! TTCAACAAGATATGTTCTAT+TGG + chr1_1:73311302-73311321 Msa0042450:intron 25.0%
! GAAGACTATCTATTGTTTAA+GGG - chr1_1:73311797-73311816 None:intergenic 25.0%
! AGAAGACTATCTATTGTTTA+AGG - chr1_1:73311798-73311817 None:intergenic 25.0%
!!! TTCCAACTTTTTTTTTTTGG+TGG - chr1_1:73312033-73312052 None:intergenic 25.0%
! ACCAAAAAAAAAAAGTTGGA+AGG + chr1_1:73312032-73312051 Msa0042450:intron 25.0%
!!! TCCTTCCAACTTTTTTTTTT+TGG - chr1_1:73312036-73312055 None:intergenic 25.0%
!! AAAAAAAGTTGGAAGGATTT+TGG + chr1_1:73312039-73312058 Msa0042450:intron 25.0%
!! AAAAAAGTTGGAAGGATTTT+GGG + chr1_1:73312040-73312059 Msa0042450:intron 25.0%
! ATTGTGAAGAAACAGATAAA+AGG + chr1_1:73313383-73313402 Msa0042450:CDS 25.0%
! TTGTGAAGAAACAGATAAAA+GGG + chr1_1:73313384-73313403 Msa0042450:CDS 25.0%
AACAATAACTCAACTCCAAT+TGG + chr1_1:73310769-73310788 Msa0042450:CDS 30.0%
TGTTCCTATTGAAGAATATG+AGG + chr1_1:73310792-73310811 Msa0042450:CDS 30.0%
TAACAGTAATCTTGTGCATA+AGG + chr1_1:73310996-73311015 Msa0042450:intron 30.0%
TGTGCATAAGGAATAGTATT+TGG + chr1_1:73311008-73311027 Msa0042450:intron 30.0%
TAATCTACTGTCATATTTGG+AGG + chr1_1:73311047-73311066 Msa0042450:intron 30.0%
!! ACTTTGAAGCATTTGCAATA+TGG - chr1_1:73311104-73311123 None:intergenic 30.0%
AAATGCTTCAAAGTCTAGAA+CGG + chr1_1:73311110-73311129 Msa0042450:intron 30.0%
AAATCAGGAAATTGCTCATT+AGG - chr1_1:73311216-73311235 None:intergenic 30.0%
!! TCATGTCTCTTTAAAATCCA+AGG - chr1_1:73311343-73311362 None:intergenic 30.0%
TTATGCAGATGATTGTTCTT+GGG + chr1_1:73311436-73311455 Msa0042450:intron 30.0%
ACTATGTATAAAAGGTGGTT+TGG + chr1_1:73311963-73311982 Msa0042450:intron 30.0%
GACAATGCATAAAATATGCA+AGG + chr1_1:73311985-73312004 Msa0042450:intron 30.0%
!!! CAACTTTTTTTTTTTGGTGG+TGG - chr1_1:73312030-73312049 None:intergenic 30.0%
CACCACCAAAAAAAAAAAGT+TGG + chr1_1:73312028-73312047 Msa0042450:intron 30.0%
! CTTGATTAGTTAGACAATGA+CGG - chr1_1:73312391-73312410 None:intergenic 30.0%
AACTACCATCAACAAACAAA+CGG + chr1_1:73312523-73312542 Msa0042450:intron 30.0%
TGGTTCAATACTCTAATATG+CGG + chr1_1:73312547-73312566 Msa0042450:intron 30.0%
ATACTCTAATATGCGGAAAA+TGG + chr1_1:73312554-73312573 Msa0042450:intron 30.0%
ACTTTCTTCTTCTTCTTCTT+CGG - chr1_1:73312660-73312679 None:intergenic 30.0%
CATTGTATGGACATAGAAAA+TGG - chr1_1:73312775-73312794 None:intergenic 30.0%
! TTTTCTATGTCCATACAATG+TGG + chr1_1:73312775-73312794 Msa0042450:CDS 30.0%
TTAAACAATATCAGAGGCTA+TGG + chr1_1:73312984-73313003 Msa0042450:CDS 30.0%
GCAAATACTACTATTCAAAG+CGG + chr1_1:73313070-73313089 Msa0042450:CDS 30.0%
AGAAGTTACGTAGGTTAAAA+AGG + chr1_1:73313161-73313180 Msa0042450:CDS 30.0%
TTAACGTTGTCAATAAGATC+AGG - chr1_1:73313340-73313359 None:intergenic 30.0%
TATTGACAACGTTAAGATGA+AGG + chr1_1:73313345-73313364 Msa0042450:CDS 30.0%
ATGATAAGAAGATCCACGAT+TGG - chr1_1:73310548-73310567 None:intergenic 35.0%
TCTTCAATAGGAACACCAAT+TGG - chr1_1:73310787-73310806 None:intergenic 35.0%
AATGAGCTGAATTAGGGTTA+GGG - chr1_1:73310831-73310850 None:intergenic 35.0%
AAATGAGCTGAATTAGGGTT+AGG - chr1_1:73310832-73310851 None:intergenic 35.0%
CAGTCAAATGAGCTGAATTA+GGG - chr1_1:73310837-73310856 None:intergenic 35.0%
TCAGTCAAATGAGCTGAATT+AGG - chr1_1:73310838-73310857 None:intergenic 35.0%
TTCACAATCGTAACTTCTGA+TGG + chr1_1:73311146-73311165 Msa0042450:intron 35.0%
TCACAATCGTAACTTCTGAT+GGG + chr1_1:73311147-73311166 Msa0042450:intron 35.0%
!! GGGGTATTTTATGAAGTTTG+AGG + chr1_1:73311168-73311187 Msa0042450:intron 35.0%
GGATATTATTATCGTGTCCT+TGG + chr1_1:73311323-73311342 Msa0042450:intron 35.0%
AGACATGAACAATAGTCTAG+TGG + chr1_1:73311355-73311374 Msa0042450:intron 35.0%
GTTATGCAGATGATTGTTCT+TGG + chr1_1:73311435-73311454 Msa0042450:intron 35.0%
TGCATAAAATATGCAAGGTC+CGG + chr1_1:73311990-73312009 Msa0042450:intron 35.0%
GACAAAATCCAAGAAACCAA+TGG - chr1_1:73312611-73312630 None:intergenic 35.0%
TGACAATGTTGAAGACCAAA+TGG + chr1_1:73312724-73312743 Msa0042450:CDS 35.0%
!! TTTTGAGCGTGTTGTTATAG+TGG - chr1_1:73312933-73312952 None:intergenic 35.0%
CTGGATTACATTAAACAGCA+TGG + chr1_1:73313043-73313062 Msa0042450:CDS 35.0%
TTGAATTCCACGCTGAATTT+GGG - chr1_1:73313124-73313143 None:intergenic 35.0%
AAGGAAACACAAGATGACTT+TGG + chr1_1:73313180-73313199 Msa0042450:CDS 35.0%
TGAGGGATCTTGAAAGAAAT+CGG - chr1_1:73313223-73313242 None:intergenic 35.0%
CTTGTGGGAAATTTCGAAAA+TGG - chr1_1:73313254-73313273 None:intergenic 35.0%
TCGAAATTTCCCACAAGATT+TGG + chr1_1:73313257-73313276 Msa0042450:CDS 35.0%
CGAAATTTCCCACAAGATTT+GGG + chr1_1:73313258-73313277 Msa0042450:CDS 35.0%
GAAATTTCCCACAAGATTTG+GGG + chr1_1:73313259-73313278 Msa0042450:CDS 35.0%
ATTCATCAACTTCCAAAACG+TGG - chr1_1:73313297-73313316 None:intergenic 35.0%
TCAATAAGATCAGGACTTTC+AGG - chr1_1:73313331-73313350 None:intergenic 35.0%
ACGTGAACACAAAAAATGAC+CGG + chr1_1:73313440-73313459 Msa0042450:CDS 35.0%
ACAAAAAATGACCGGAACAA+TGG + chr1_1:73313448-73313467 Msa0042450:CDS 35.0%
! TTGTTTCTCACCATTGTTGA+AGG + chr1_1:73313516-73313535 Msa0042450:CDS 35.0%
ACCATTGTTGAAGGAACTAT+TGG + chr1_1:73313525-73313544 Msa0042450:CDS 35.0%
TCCAATAGTTCCTTCAACAA+TGG - chr1_1:73313529-73313548 None:intergenic 35.0%
GGGATGAACAATGGTTAAAA+CGG + chr1_1:73313608-73313627 Msa0042450:CDS 35.0%
ATGAACAATGGTTAAAACGG+AGG + chr1_1:73313611-73313630 Msa0042450:CDS 35.0%
! ATGGTTAAAACGGAGGATTT+TGG + chr1_1:73313618-73313637 Msa0042450:CDS 35.0%
! AACTGGAGGTGTATTTGAAT+CGG + chr1_1:73313641-73313660 Msa0042450:CDS 35.0%
! AGAAGCTTTTCCAATGTCTC+TGG + chr1_1:73310473-73310492 Msa0042450:CDS 40.0%
CTTCTTTAGTCCAGAGACAT+TGG - chr1_1:73310486-73310505 None:intergenic 40.0%
! CTTATCAACAAGCTAGCTCT+TGG - chr1_1:73310626-73310645 None:intergenic 40.0%
!! AGCTTGTTGATAAGCTTCGA+AGG + chr1_1:73310632-73310651 Msa0042450:intron 40.0%
CTGAATTAGGGTTAGGGTTT+GGG - chr1_1:73310825-73310844 None:intergenic 40.0%
TACGATTGTGAACGATGGTT+AGG - chr1_1:73311138-73311157 None:intergenic 40.0%
GAAGTTACGATTGTGAACGA+TGG - chr1_1:73311143-73311162 None:intergenic 40.0%
CACAATCGTAACTTCTGATG+GGG + chr1_1:73311148-73311167 Msa0042450:intron 40.0%
ACAATCGTAACTTCTGATGG+GGG + chr1_1:73311149-73311168 Msa0042450:intron 40.0%
GCTCATTAGGCATTTCAGAT+CGG - chr1_1:73311203-73311222 None:intergenic 40.0%
CTGAGATGATTCTCATGTGT+GGG + chr1_1:73311402-73311421 Msa0042450:intron 40.0%
GCATAAAATATGCAAGGTCC+GGG + chr1_1:73311991-73312010 Msa0042450:intron 40.0%
CATAAAATATGCAAGGTCCG+GGG + chr1_1:73311992-73312011 Msa0042450:intron 40.0%
!!! TTTTTTTTTGGTGGTGGCCA+GGG - chr1_1:73312024-73312043 None:intergenic 40.0%
!!! TTTTTTTTTTGGTGGTGGCC+AGG - chr1_1:73312025-73312044 None:intergenic 40.0%
TCTTCAGGGACGTTACATTA+CGG + chr1_1:73312155-73312174 Msa0042450:intron 40.0%
TCTTCAGGGACGTTACATTA+CGG + chr1_1:73312155-73312174 Msa0042450:intron 40.0%
CGAAGGTTAAGGTTTCAAGA+AGG - chr1_1:73312358-73312377 None:intergenic 40.0%
! GTCGTTGATTACGAAGGTTA+AGG - chr1_1:73312369-73312388 None:intergenic 40.0%
ACCATCAACAAACAAACGGT+TGG + chr1_1:73312527-73312546 Msa0042450:intron 40.0%
! ACCAACCGTTTGTTTGTTGA+TGG - chr1_1:73312531-73312550 None:intergenic 40.0%
!! CAATGGTGAAAGCATTGTTG+AGG - chr1_1:73312594-73312613 None:intergenic 40.0%
CTCAACAATGCTTTCACCAT+TGG + chr1_1:73312592-73312611 Msa0042450:intron 40.0%
!! TGCTTTCACCATTGGTTTCT+TGG + chr1_1:73312600-73312619 Msa0042450:intron 40.0%
TGAAGACCAAATGGACAATG+TGG + chr1_1:73312733-73312752 Msa0042450:CDS 40.0%
CATCATCCACATTGTCCATT+TGG - chr1_1:73312742-73312761 None:intergenic 40.0%
! CAATGTGGATGATGACGAAA+AGG + chr1_1:73312748-73312767 Msa0042450:CDS 40.0%
TGGTCTTCATCCACATTGTA+TGG - chr1_1:73312788-73312807 None:intergenic 40.0%
CAATGTGGATGAAGACCAAA+TGG + chr1_1:73312790-73312809 Msa0042450:CDS 40.0%
CAATGGGAATGTTGTCCATT+TGG - chr1_1:73312808-73312827 None:intergenic 40.0%
ACGGCGTTAAACAATATCAG+AGG + chr1_1:73312978-73312997 Msa0042450:CDS 40.0%
GACGAGATTGAACTGTTGAA+GGG + chr1_1:73313016-73313035 Msa0042450:CDS 40.0%
TGAACTGTTGAAGGGATATC+TGG + chr1_1:73313024-73313043 Msa0042450:CDS 40.0%
AGTTATTCCCAAATTCAGCG+TGG + chr1_1:73313114-73313133 Msa0042450:CDS 40.0%
GTTGAATTCCACGCTGAATT+TGG - chr1_1:73313125-73313144 None:intergenic 40.0%
AATTCAGCGTGGAATTCAAC+AGG + chr1_1:73313125-73313144 Msa0042450:CDS 40.0%
TACGTAACTTCTCAACAAGC+TGG - chr1_1:73313153-73313172 None:intergenic 40.0%
!! AGCTTGTTGAGAAGTTACGT+AGG + chr1_1:73313152-73313171 Msa0042450:CDS 40.0%
CACAAGATGACTTTGGACAA+AGG + chr1_1:73313187-73313206 Msa0042450:CDS 40.0%
GATTTCTTTCAAGATCCCTC+AGG + chr1_1:73313222-73313241 Msa0042450:CDS 40.0%
ATCAACTTCCAAAACGTGGT+CGG - chr1_1:73313293-73313312 None:intergenic 40.0%
TAGTAGCATTGCCATTGTTC+CGG - chr1_1:73313462-73313481 None:intergenic 40.0%
ATGGCAATGCTACTAGCATA+CGG + chr1_1:73313467-73313486 Msa0042450:CDS 40.0%
TGGTGAGAAACAAGACTTCA+TGG - chr1_1:73313509-73313528 None:intergenic 40.0%
TCCCTTGTCGATAACAACAT+CGG - chr1_1:73313583-73313602 None:intergenic 40.0%
!!! AAAACGGAGGATTTTGGAAC+TGG + chr1_1:73313624-73313643 Msa0042450:CDS 40.0%
GACGAGATTACGATCTTGCA+AGG + chr1_1:73310505-73310524 Msa0042450:CDS 45.0%
GCTGAATTAGGGTTAGGGTT+TGG - chr1_1:73310826-73310845 None:intergenic 45.0%
GAGATTGCGAGCATCTTAGT+AGG + chr1_1:73310882-73310901 Msa0042450:intron 45.0%
GCTGAGATGATTCTCATGTG+TGG + chr1_1:73311401-73311420 Msa0042450:intron 45.0%
AGACATTTGTGTGACCATCC+TGG - chr1_1:73311474-73311493 None:intergenic 45.0%
TTCAAGAAGGCTTAGAGCCT+CGG - chr1_1:73312345-73312364 None:intergenic 45.0%
! ATGACGGTCGTTGATTACGA+AGG - chr1_1:73312375-73312394 None:intergenic 45.0%
TTCTTCTTCGGATGACGAAG+AGG - chr1_1:73312648-73312667 None:intergenic 45.0%
TCAGAAACGGCAAGTGCAAT+GGG - chr1_1:73312824-73312843 None:intergenic 45.0%
ATCAGAAACGGCAAGTGCAA+TGG - chr1_1:73312825-73312844 None:intergenic 45.0%
GGACGAGATTGAACTGTTGA+AGG + chr1_1:73313015-73313034 Msa0042450:CDS 45.0%
GACTTTGGACAAAGGCAAAG+AGG + chr1_1:73313195-73313214 Msa0042450:CDS 45.0%
!! CGAAAATGGCTTGTTCCTGA+GGG - chr1_1:73313240-73313259 None:intergenic 45.0%
!! TCGAAAATGGCTTGTTCCTG+AGG - chr1_1:73313241-73313260 None:intergenic 45.0%
GTCATTGCCCCAAATCTTGT+GGG - chr1_1:73313269-73313288 None:intergenic 45.0%
TGTCATTGCCCCAAATCTTG+TGG - chr1_1:73313270-73313289 None:intergenic 45.0%
AGATCAGGACTTTCAGGAAC+AGG - chr1_1:73313325-73313344 None:intergenic 45.0%
TGGCAATGCTACTAGCATAC+GGG + chr1_1:73313468-73313487 Msa0042450:CDS 45.0%
GCCGATGTTGTTATCGACAA+GGG + chr1_1:73313579-73313598 Msa0042450:CDS 45.0%
TGTTATCGACAAGGGAAGTG+AGG + chr1_1:73313587-73313606 Msa0042450:CDS 45.0%
GTTATCGACAAGGGAAGTGA+GGG + chr1_1:73313588-73313607 Msa0042450:CDS 45.0%
! GGAGGTGTATTTGAATCGGT+TGG + chr1_1:73313645-73313664 Msa0042450:CDS 45.0%
AATCGGTTGGAGTTGTTGCA+AGG + chr1_1:73313658-73313677 Msa0042450:CDS 45.0%
GTTGTTGCAAGGTCAGATCA+AGG + chr1_1:73313669-73313688 Msa0042450:CDS 45.0%
!!! AATAAATTATTATTTTTTGA+AGG - chr1_1:73311517-73311536 None:intergenic 5.0%
CTCGATTACAACGCCAATCG+TGG + chr1_1:73310532-73310551 Msa0042450:CDS 50.0%
CTTGGGCAATGATACTGACC+AGG + chr1_1:73311453-73311472 Msa0042450:intron 50.0%
GGCAATGATACTGACCAGGA+TGG + chr1_1:73311457-73311476 Msa0042450:intron 50.0%
TTAGAGCCTCGGCTTCTGAA+GGG - chr1_1:73312334-73312353 None:intergenic 50.0%
AACGGGGGATGCATCAGAAA+CGG - chr1_1:73312837-73312856 None:intergenic 50.0%
TGCCGTGTTTGTGTCATCTG+CGG - chr1_1:73312882-73312901 None:intergenic 50.0%
TATAGTGGTAGTAGCCACCG+TGG - chr1_1:73312918-73312937 None:intergenic 50.0%
TCAGAGGCTATGGACAAAGC+AGG + chr1_1:73312994-73313013 Msa0042450:CDS 50.0%
! CAATGACACCGACCACGTTT+TGG + chr1_1:73313282-73313301 Msa0042450:CDS 50.0%
GGCAATGCTACTAGCATACG+GGG + chr1_1:73313469-73313488 Msa0042450:CDS 50.0%
CGCCGATGTTGTTATCGACA+AGG + chr1_1:73313578-73313597 Msa0042450:CDS 50.0%
GGGAAGTGAGGGATGAACAA+TGG + chr1_1:73313599-73313618 Msa0042450:CDS 50.0%
!!! ACGGAGGATTTTGGAACTGG+AGG + chr1_1:73313627-73313646 Msa0042450:CDS 50.0%
TGCAAGGTCAGATCAAGGCT+AGG + chr1_1:73313674-73313693 Msa0042450:CDS 50.0%
AGGTCAGATCAAGGCTAGGT+TGG + chr1_1:73313678-73313697 Msa0042450:CDS 50.0%
! TCAGATCAAGGCTAGGTTGG+AGG + chr1_1:73313681-73313700 Msa0042450:CDS 50.0%
ATCTTCCCCTTCAGAAGCCG+AGG + chr1_1:73312325-73312344 Msa0042450:intron 55.0%
! TAGAGCCTCGGCTTCTGAAG+GGG - chr1_1:73312333-73312352 None:intergenic 55.0%
CTTAGAGCCTCGGCTTCTGA+AGG - chr1_1:73312335-73312354 None:intergenic 55.0%
TGATGCATCCCCCGTTGTGA+CGG + chr1_1:73312841-73312860 Msa0042450:CDS 55.0%
CGTGTTTGTGTCATCTGCGG+GGG - chr1_1:73312879-73312898 None:intergenic 55.0%
CCGTGTTTGTGTCATCTGCG+GGG - chr1_1:73312880-73312899 None:intergenic 55.0%
CCCCGCAGATGACACAAACA+CGG + chr1_1:73312877-73312896 Msa0042450:CDS 55.0%
GCCGTGTTTGTGTCATCTGC+GGG - chr1_1:73312881-73312900 None:intergenic 55.0%
GGCAACAAACACAGCCACCA+CGG + chr1_1:73312898-73312917 Msa0042450:CDS 55.0%
AACAAACACAGCCACCACGG+TGG + chr1_1:73312901-73312920 Msa0042450:CDS 55.0%
AACAACATCGGCGACGCACT+GGG - chr1_1:73313571-73313590 None:intergenic 55.0%
TAACAACATCGGCGACGCAC+TGG - chr1_1:73313572-73313591 None:intergenic 55.0%
AAGGTCCGGGGTTCAAACCC+TGG + chr1_1:73312004-73312023 Msa0042450:intron 60.0%
AGCCATGGCCACCGTCACAA+CGG - chr1_1:73312855-73312874 None:intergenic 60.0%
AGTGGTAGTAGCCACCGTGG+TGG - chr1_1:73312915-73312934 None:intergenic 60.0%
GGTGGCCAGGGTTTGAACCC+CGG - chr1_1:73312012-73312031 None:intergenic 65.0%
TGCATCCCCCGTTGTGACGG+TGG + chr1_1:73312844-73312863 Msa0042450:CDS 65.0%
CATGGCCACCGTCACAACGG+GGG - chr1_1:73312852-73312871 None:intergenic 65.0%
CCATGGCCACCGTCACAACG+GGG - chr1_1:73312853-73312872 None:intergenic 65.0%
GCCATGGCCACCGTCACAAC+GGG - chr1_1:73312854-73312873 None:intergenic 65.0%
! GACGCACTGGGTGGCTCTAC+TGG - chr1_1:73313559-73313578 None:intergenic 65.0%
AACATCGGCGACGCACTGGG+TGG - chr1_1:73313568-73313587 None:intergenic 65.0%
CCCCGTTGTGACGGTGGCCA+TGG + chr1_1:73312850-73312869 Msa0042450:CDS 70.0%
GTCATCTGCGGGGGCAGCCA+TGG - chr1_1:73312870-73312889 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 73310448 73313726 73310448 ID=Msa0042450;Name=Msa0042450
chr1_1 mRNA 73310448 73313726 73310448 ID=Msa0042450-mRNA-1;Parent=Msa0042450;Name=Msa0042450-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|0.33|0.25|4|0|477
chr1_1 exon 73310448 73310618 73310448 ID=Msa0042450-mRNA-1:exon:24521;Parent=Msa0042450-mRNA-1
chr1_1 exon 73310763 73310864 73310763 ID=Msa0042450-mRNA-1:exon:24522;Parent=Msa0042450-mRNA-1
chr1_1 exon 73310904 73311000 73310904 ID=Msa0042450-mRNA-1:exon:24523;Parent=Msa0042450-mRNA-1
chr1_1 exon 73312663 73313726 73312663 ID=Msa0042450-mRNA-1:exon:24524;Parent=Msa0042450-mRNA-1
chr1_1 CDS 73310448 73310618 73310448 ID=Msa0042450-mRNA-1:cds;Parent=Msa0042450-mRNA-1
chr1_1 CDS 73310763 73310864 73310763 ID=Msa0042450-mRNA-1:cds;Parent=Msa0042450-mRNA-1
chr1_1 CDS 73310904 73311000 73310904 ID=Msa0042450-mRNA-1:cds;Parent=Msa0042450-mRNA-1
chr1_1 CDS 73312663 73313726 73312663 ID=Msa0042450-mRNA-1:cds;Parent=Msa0042450-mRNA-1
Gene Sequence

>Msa0042450

ATGGAAGAGAAGAACAACGATTCACAGAAGCTTTTCCAATGTCTCTGGACTAAAGAAGACGAGATTACGATCTTGCAAGGTTTGCTCGATTACAACGCCAATCGTGGATCTTCTTATCATAATGATATTGGATCGTTCTATGATCAAATTAAGGCAAAGATTCAACTCGATAACTGTAACAATAACTCAACTCCAATTGGTGTTCCTATTGAAGAATATGAGGAAATCATCCCAAACCCTAACCCTAATTCAGCTCATTTGACTGAGAAGAAAGAGAAGCGTGAGTTGAATGATTCTAAAGACAATCATCATAATAATTCAAGTAATAACAATAATAGCAATGTTGAAGAAAATGCCGATAAGAGTAACAAAGAAGAAGAAGAAAGTCTTGACATTAACAATGACGAAGCAGACTATGAAAACTTTGAAAATGACAATGTTGAAGACCAAATGGACAATGTGGATGATGACGAAAAGGACCATTTTCTATGTCCATACAATGTGGATGAAGACCAAATGGACAACATTCCCATTGCACTTGCCGTTTCTGATGCATCCCCCGTTGTGACGGTGGCCATGGCTGCCCCCGCAGATGACACAAACACGGCAACAAACACAGCCACCACGGTGGCTACTACCACTATAACAACACGCTCAAAACGACAATATAACAAAAAGTATTCAGACGGCGTTAAACAATATCAGAGGCTATGGACAAAGCAGGACGAGATTGAACTGTTGAAGGGATATCTGGATTACATTAAACAGCATGGAAGAGCAAATACTACTATTCAAAGCGGCGTAGCTTCATTCTATGATCAAGTTATTCCCAAATTCAGCGTGGAATTCAACAGGAACCAGCTTGTTGAGAAGTTACGTAGGTTAAAAAGGAAACACAAGATGACTTTGGACAAAGGCAAAGAGGTTCCGATTTCTTTCAAGATCCCTCAGGAACAAGCCATTTTCGAAATTTCCCACAAGATTTGGGGCAATGACACCGACCACGTTTTGGAAGTTGATGAATCAAGACCTGTTCCTGAAAGTCCTGATCTTATTGACAACGTTAAGATGAAGGATGAAGATGTTGACGATTGTGAAGAAACAGATAAAAGGGCGCACAAGCGTGTGAAGATAACAACAGATGACGACGTGAACACAAAAAATGACCGGAACAATGGCAATGCTACTAGCATACGGGGCTTCATTGAAGAAACCATGAAGTCTTGTTTCTCACCATTGTTGAAGGAACTATTGGATGATGTTCCAGTAGAGCCACCCAGTGCGTCGCCGATGTTGTTATCGACAAGGGAAGTGAGGGATGAACAATGGTTAAAACGGAGGATTTTGGAACTGGAGGTGTATTTGAATCGGTTGGAGTTGTTGCAAGGTCAGATCAAGGCTAGGTTGGAGGAGTTAAAGTCTTGCTTAGATTGA
Protein sequence

>Msa0042450

MEEKNNDSQKLFQCLWTKEDEITILQGLLDYNANRGSSYHNDIGSFYDQIKAKIQLDNCNNNSTPIGVPIEEYEEIIPNPNPNSAHLTEKKEKRELNDSKDNHHNNSSNNNNSNVEENADKSNKEEEESLDINNDEADYENFENDNVEDQMDNVDDDEKDHFLCPYNVDEDQMDNIPIALAVSDASPVVTVAMAAPADDTNTATNTATTVATTTITTRSKRQYNKKYSDGVKQYQRLWTKQDEIELLKGYLDYIKQHGRANTTIQSGVASFYDQVIPKFSVEFNRNQLVEKLRRLKRKHKMTLDKGKEVPISFKIPQEQAIFEISHKIWGNDTDHVLEVDESRPVPESPDLIDNVKMKDEDVDDCEETDKRAHKRVKITTDDDVNTKNDRNNGNATSIRGFIEETMKSCFSPLLKELLDDVPVEPPSASPMLLSTREVRDEQWLKRRILELEVYLNRLELLQGQIKARLEELKSCLD*