Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0043410 | sp|Q9FJ10|GAT16_ARATH | 49.412 | 85 | 32 | 2 | 15 | 96 | 32 | 108 | 1.35e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0043410 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0043410 | MtrunA17_Chr4g0068031 | 74.757 | 103 | 21 | 2 | 5 | 102 | 16 | 118 | 1.68e-36 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0043410 | AT5G49300.1 | 49.412 | 85 | 32 | 2 | 15 | 96 | 32 | 108 | 1.37e-15 | 67.8 |
| Msa0043410 | AT3G06740.1 | 45.055 | 91 | 37 | 3 | 15 | 102 | 37 | 117 | 3.62e-15 | 67.0 |
| Msa0043410 | AT4G16141.1 | 63.265 | 49 | 15 | 1 | 16 | 61 | 34 | 82 | 1.90e-13 | 63.2 |
| Msa0043410 | AT3G16870.1 | 48.611 | 72 | 30 | 2 | 16 | 84 | 39 | 106 | 5.48e-12 | 59.3 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAAGATCATTGAATTTA+AGG | 0.099753 | 1_1:-74571818 | Msa0043410:CDS |
| AACAAAGCTAGTAAAGTTTA+TGG | 0.213203 | 1_1:+74572171 | None:intergenic |
| AAGAAGAAGAGAGCAATATT+AGG | 0.216353 | 1_1:-74571936 | Msa0043410:CDS |
| ACCACGACATTGAGGAGTTT+TGG | 0.295427 | 1_1:+74572099 | None:intergenic |
| TCTCTTCTTCTTCCTGCTCC+TGG | 0.356990 | 1_1:+74571947 | None:intergenic |
| GCGGCGGCGGAAGCAAAGTT+GGG | 0.384378 | 1_1:-74571860 | Msa0043410:CDS |
| AGAAGAAGAGAGCAATATTA+GGG | 0.387155 | 1_1:-74571935 | Msa0043410:CDS |
| GGCGGCGGCGGAAGCAAAGT+TGG | 0.388449 | 1_1:-74571861 | Msa0043410:CDS |
| AACGAAGAAGGAACAAGAAA+GGG | 0.398579 | 1_1:-74571897 | Msa0043410:CDS |
| AGAGAGCAATATTAGGGATA+AGG | 0.418283 | 1_1:-74571929 | Msa0043410:CDS |
| ATGCTTGCGGGACCAGGAGC+AGG | 0.445349 | 1_1:-74571959 | Msa0043410:CDS |
| CAACGAAGAAGGAACAAGAA+AGG | 0.448446 | 1_1:-74571898 | Msa0043410:CDS |
| AATAAGAGTAACGGCGGCGG+CGG | 0.473501 | 1_1:-74571873 | Msa0043410:CDS |
| TGTGCAATGCTTGCGGGACC+AGG | 0.480073 | 1_1:-74571965 | Msa0043410:CDS |
| GGACGTTCCGCAACCGGCAC+AGG | 0.481338 | 1_1:+74572120 | None:intergenic |
| AAGAAAACCTGTGCCGGTTG+CGG | 0.485045 | 1_1:-74572127 | Msa0043410:CDS |
| AGTCACTGTGCAATGCTTGC+GGG | 0.493499 | 1_1:-74571971 | Msa0043410:CDS |
| AGGAAAGGAAACAACGAAGA+AGG | 0.523485 | 1_1:-74571909 | Msa0043410:CDS |
| CGGCGGCGGAAGCAAAGTTG+GGG | 0.548379 | 1_1:-74571859 | Msa0043410:CDS |
| AATCCAAAGAAAACCTGTGC+CGG | 0.567940 | 1_1:-74572133 | Msa0043410:CDS |
| GCAATATTAGGGATAAGGAA+AGG | 0.573561 | 1_1:-74571924 | Msa0043410:CDS |
| CAGTCACTGTGCAATGCTTG+CGG | 0.581160 | 1_1:-74571972 | Msa0043410:CDS |
| TTTGGACCACCACGACATTG+AGG | 0.585914 | 1_1:+74572091 | None:intergenic |
| GGGAATAAGAGTAACGGCGG+CGG | 0.586228 | 1_1:-74571876 | Msa0043410:CDS |
| TCCAAAACTCCTCAATGTCG+TGG | 0.628200 | 1_1:-74572100 | Msa0043410:CDS |
| ACGAAGAAGGAACAAGAAAG+GGG | 0.636309 | 1_1:-74571896 | Msa0043410:CDS |
| GGCGGCGGAAGCAAAGTTGG+GGG | 0.642920 | 1_1:-74571858 | Msa0043410:CDS |
| AGAAAGGGGAATAAGAGTAA+CGG | 0.665248 | 1_1:-74571882 | Msa0043410:CDS |
| AAAACTCCTCAATGTCGTGG+TGG | 0.684546 | 1_1:-74572097 | Msa0043410:CDS |
| AAGGGGAATAAGAGTAACGG+CGG | 0.727683 | 1_1:-74571879 | Msa0043410:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAAGATCATTGAATTTA+AGG | - | chr1_1:74572154-74572173 | Msa0043410:CDS | 20.0% |
| ! | AACAAAGCTAGTAAAGTTTA+TGG | + | chr1_1:74571804-74571823 | None:intergenic | 25.0% |
| !! | TGATTAGCGTTAATTACTTT+TGG | + | chr1_1:74571902-74571921 | None:intergenic | 25.0% |
| AAGAAGAAGAGAGCAATATT+AGG | - | chr1_1:74572036-74572055 | Msa0043410:intron | 30.0% | |
| AGAAGAAGAGAGCAATATTA+GGG | - | chr1_1:74572037-74572056 | Msa0043410:intron | 30.0% | |
| AGAGAGCAATATTAGGGATA+AGG | - | chr1_1:74572043-74572062 | Msa0043410:intron | 35.0% | |
| GCAATATTAGGGATAAGGAA+AGG | - | chr1_1:74572048-74572067 | Msa0043410:intron | 35.0% | |
| AACGAAGAAGGAACAAGAAA+GGG | - | chr1_1:74572075-74572094 | Msa0043410:intron | 35.0% | |
| AGAAAGGGGAATAAGAGTAA+CGG | - | chr1_1:74572090-74572109 | Msa0043410:CDS | 35.0% | |
| AATCCAAAGAAAACCTGTGC+CGG | - | chr1_1:74571839-74571858 | Msa0043410:CDS | 40.0% | |
| AGGAAAGGAAACAACGAAGA+AGG | - | chr1_1:74572063-74572082 | Msa0043410:intron | 40.0% | |
| CAACGAAGAAGGAACAAGAA+AGG | - | chr1_1:74572074-74572093 | Msa0043410:intron | 40.0% | |
| ACGAAGAAGGAACAAGAAAG+GGG | - | chr1_1:74572076-74572095 | Msa0043410:intron | 40.0% | |
| TCCAAAACTCCTCAATGTCG+TGG | - | chr1_1:74571872-74571891 | Msa0043410:CDS | 45.0% | |
| ! | ACCACGACATTGAGGAGTTT+TGG | + | chr1_1:74571876-74571895 | None:intergenic | 45.0% |
| AAAACTCCTCAATGTCGTGG+TGG | - | chr1_1:74571875-74571894 | Msa0043410:CDS | 45.0% | |
| AAGGGGAATAAGAGTAACGG+CGG | - | chr1_1:74572093-74572112 | Msa0043410:CDS | 45.0% | |
| ! | CAACCGGCACAGGTTTTCTT+TGG | + | chr1_1:74571845-74571864 | None:intergenic | 50.0% |
| !! | AAGAAAACCTGTGCCGGTTG+CGG | - | chr1_1:74571845-74571864 | Msa0043410:CDS | 50.0% |
| !!! | AGTTTTGGACGTTCCGCAAC+CGG | + | chr1_1:74571861-74571880 | None:intergenic | 50.0% |
| TTTGGACCACCACGACATTG+AGG | + | chr1_1:74571884-74571903 | None:intergenic | 50.0% | |
| CAGTCACTGTGCAATGCTTG+CGG | - | chr1_1:74572000-74572019 | Msa0043410:intron | 50.0% | |
| AGTCACTGTGCAATGCTTGC+GGG | - | chr1_1:74572001-74572020 | Msa0043410:intron | 50.0% | |
| TCTCTTCTTCTTCCTGCTCC+TGG | + | chr1_1:74572028-74572047 | None:intergenic | 50.0% | |
| GGGAATAAGAGTAACGGCGG+CGG | - | chr1_1:74572096-74572115 | Msa0043410:CDS | 55.0% | |
| AATAAGAGTAACGGCGGCGG+CGG | - | chr1_1:74572099-74572118 | Msa0043410:CDS | 55.0% | |
| TGTGCAATGCTTGCGGGACC+AGG | - | chr1_1:74572007-74572026 | Msa0043410:intron | 60.0% | |
| ATGCTTGCGGGACCAGGAGC+AGG | - | chr1_1:74572013-74572032 | Msa0043410:intron | 65.0% | |
| GCGGCGGCGGAAGCAAAGTT+GGG | - | chr1_1:74572112-74572131 | Msa0043410:CDS | 65.0% | |
| CGGCGGCGGAAGCAAAGTTG+GGG | - | chr1_1:74572113-74572132 | Msa0043410:CDS | 65.0% | |
| GGCGGCGGAAGCAAAGTTGG+GGG | - | chr1_1:74572114-74572133 | Msa0043410:CDS | 65.0% | |
| GGACGTTCCGCAACCGGCAC+AGG | + | chr1_1:74571855-74571874 | None:intergenic | 70.0% | |
| GGCGGCGGCGGAAGCAAAGT+TGG | - | chr1_1:74572111-74572130 | Msa0043410:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 74571794 | 74572200 | 74571794 | ID=Msa0043410;Name=Msa0043410 |
| chr1_1 | mRNA | 74571794 | 74572200 | 74571794 | ID=Msa0043410-mRNA-1;Parent=Msa0043410;Name=Msa0043410-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|1|1|1|2|0|102 |
| chr1_1 | exon | 74572090 | 74572200 | 74572090 | ID=Msa0043410-mRNA-1:exon:25135;Parent=Msa0043410-mRNA-1 |
| chr1_1 | exon | 74571794 | 74571991 | 74571794 | ID=Msa0043410-mRNA-1:exon:25134;Parent=Msa0043410-mRNA-1 |
| chr1_1 | CDS | 74572090 | 74572200 | 74572090 | ID=Msa0043410-mRNA-1:cds;Parent=Msa0043410-mRNA-1 |
| chr1_1 | CDS | 74571794 | 74571991 | 74571794 | ID=Msa0043410-mRNA-1:cds;Parent=Msa0043410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
