AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0049700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0049700 MtrunA17_Chr2g0330591 56.873 371 45 6 1 262 282 646 3.63e-125 376
Msa0049700 MtrunA17_Chr2g0330631 71.519 158 16 1 134 262 2 159 2.04e-72 222
Msa0049700 MtrunA17_Chr2g0330621 71.875 160 15 3 1 135 49 203 5.14e-71 216
Msa0049700 MtrunA17_Chr8g0341011 33.871 372 126 12 1 262 281 642 2.26e-49 174
Msa0049700 MtrunA17_Chr8g0341151 33.151 365 131 9 2 262 277 632 2.45e-49 173
Msa0049700 MtrunA17_Chr8g0340931 33.784 370 127 11 1 262 280 639 2.90e-49 173
Msa0049700 MtrunA17_Chr8g0340891 32.329 365 133 8 1 262 284 637 1.87e-48 171
Msa0049700 MtrunA17_Chr8g0340911 33.333 366 132 9 1 262 282 639 3.79e-48 170
Msa0049700 MtrunA17_Chr8g0340871 31.903 373 133 9 1 262 286 648 3.13e-46 165
Msa0049700 MtrunA17_Chr8g0347661 31.608 367 136 9 1 262 288 644 4.46e-45 161
Msa0049700 MtrunA17_Chr8g0340881 33.067 375 121 13 2 262 285 643 1.30e-42 154
Msa0049700 MtrunA17_Chr6g0487571 30.663 362 148 8 1 262 285 643 7.24e-42 152
Msa0049700 MtrunA17_Chr8g0358831 31.492 362 135 8 6 262 254 607 1.11e-38 143
Msa0049700 MtrunA17_Chr8g0341101 29.775 356 150 9 2 262 339 689 1.17e-37 140
Msa0049700 MtrunA17_Chr8g0341361 34.586 266 76 6 89 262 1 260 2.08e-33 126
Msa0049700 MtrunA17_Chr8g0341061 30.878 353 150 10 2 262 336 686 2.83e-33 127
Msa0049700 MtrunA17_Chr8g0341111 28.775 351 135 9 18 262 211 552 3.51e-30 119
Msa0049700 MtrunA17_Chr8g0340941 30.597 268 92 9 1 189 280 532 8.15e-26 105
Msa0049700 MtrunA17_Chr8g0341231 36.571 175 75 5 1 144 302 471 1.20e-23 99.8
Msa0049700 MtrunA17_Chr8g0340971 35.333 150 65 4 141 262 17 162 4.16e-20 87.0
Msa0049700 MtrunA17_Chr8g0341131 52.778 72 34 0 191 262 19 90 5.71e-19 81.6
Msa0049700 MtrunA17_Chr2g0330691 86.364 44 6 0 219 262 1 44 6.43e-19 82.8
Msa0049700 MtrunA17_Chr8g0341221 54.167 72 33 0 191 262 39 110 1.00e-17 79.3
Msa0049700 MtrunA17_Chr8g0341081 51.389 72 35 0 191 262 49 120 1.71e-17 80.5
Msa0049700 MtrunA17_Chr8g0341251 51.389 72 35 0 191 262 50 121 3.24e-17 79.7
Msa0049700 MtrunA17_Chr8g0340951 50.000 72 36 0 191 262 44 115 1.08e-16 76.6
Msa0049700 MtrunA17_Chr2g0330701 76.744 43 10 0 147 189 29 71 1.41e-16 72.8
Msa0049700 MtrunA17_Chr5g0423891 31.746 189 96 4 103 262 51 235 4.59e-13 68.6
Msa0049700 MtrunA17_Chr7g0263591 22.365 389 174 12 2 262 288 676 8.54e-13 68.2
Msa0049700 MtrunA17_Chr5g0411631 34.409 93 61 0 170 262 576 668 2.93e-12 66.2
Msa0049700 MtrunA17_Chr6g0485721 36.047 86 55 0 176 261 84 169 3.63e-11 62.8
Msa0049700 MtrunA17_Chr7g0219671 38.824 85 50 1 180 262 521 605 4.62e-11 62.8
Msa0049700 MtrunA17_Chr8g0365691 41.429 70 41 0 193 262 314 383 6.11e-11 62.0
Msa0049700 MtrunA17_Chr7g0219651 39.535 86 50 1 179 262 1507 1592 6.17e-11 62.8
Msa0049700 MtrunA17_Chr7g0219781 38.824 85 50 1 180 262 524 608 6.51e-11 62.4
Msa0049700 MtrunA17_Chr7g0219631 38.750 80 47 1 180 257 50 129 8.07e-11 58.5
Msa0049700 MtrunA17_Chr7g0219571 31.973 147 82 4 118 262 477 607 8.18e-11 62.0
Msa0049700 MtrunA17_Chr3g0123141 41.538 65 38 0 191 255 609 673 9.51e-11 62.0
Msa0049700 MtrunA17_Chr7g0219601 39.535 86 50 1 179 262 506 591 9.82e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0049700 AT2G19210.1 36.388 371 120 10 1 262 284 647 2.21e-56 194
Msa0049700 AT1G51880.2 36.585 369 119 9 1 262 295 655 2.04e-53 186
Msa0049700 AT1G51880.1 36.585 369 119 9 1 262 277 637 2.07e-53 185
Msa0049700 AT1G51870.1 36.527 334 131 7 1 262 278 602 3.68e-53 184
Msa0049700 AT2G19230.2 34.540 359 136 11 1 262 284 640 1.73e-52 183
Msa0049700 AT1G51880.3 35.809 377 119 9 1 262 295 663 2.46e-52 182
Msa0049700 AT2G19230.1 34.444 360 136 11 1 262 284 641 4.02e-52 182
Msa0049700 AT1G51805.2 32.134 389 127 9 1 262 247 625 7.93e-51 178
Msa0049700 AT1G51805.1 32.134 389 127 9 1 262 271 649 8.31e-51 178
Msa0049700 AT1G51890.5 32.973 370 139 6 1 262 1 369 5.31e-50 173
Msa0049700 AT1G51890.3 32.973 370 139 6 1 262 273 641 4.28e-49 172
Msa0049700 AT1G51890.4 32.973 370 139 6 1 262 209 577 5.77e-49 172
Msa0049700 AT1G51890.2 32.973 370 139 6 1 262 273 641 7.45e-49 172
Msa0049700 AT1G51890.1 32.973 370 139 6 1 262 273 641 1.01e-48 172
Msa0049700 AT1G51860.2 34.896 384 122 11 1 262 134 511 1.50e-47 168
Msa0049700 AT1G51870.2 33.420 383 128 8 1 262 278 654 1.94e-47 169
Msa0049700 AT1G05700.1 33.604 369 131 9 1 262 280 641 3.37e-47 168
Msa0049700 AT1G51860.1 35.156 384 121 11 1 262 278 655 3.74e-47 168
Msa0049700 AT4G29990.2 31.635 373 137 10 1 262 280 645 2.51e-45 162
Msa0049700 AT4G29990.1 31.635 373 137 10 1 262 280 645 3.08e-45 162
Msa0049700 AT3G46420.1 32.754 345 130 9 1 262 278 603 1.24e-44 160
Msa0049700 AT1G51850.2 31.088 386 137 10 1 262 270 650 8.11e-44 158
Msa0049700 AT1G51850.1 31.088 386 137 10 1 262 250 630 9.52e-44 158
Msa0049700 AT1G51810.1 30.909 385 136 10 1 262 145 522 1.21e-43 157
Msa0049700 AT1G51820.1 29.974 387 140 9 1 262 270 650 8.38e-43 155
Msa0049700 AT1G51830.1 30.287 383 141 9 1 262 81 458 1.01e-42 154
Msa0049700 AT1G51830.2 30.287 383 141 9 1 262 88 465 1.25e-42 154
Msa0049700 AT1G51820.2 29.504 383 139 9 5 262 12 388 4.50e-41 149
Msa0049700 AT1G07560.1 30.811 370 141 11 1 262 280 642 1.32e-40 149
Msa0049700 AT1G51910.1 33.333 375 130 10 1 262 278 645 1.63e-39 146
Msa0049700 AT2G28970.1 30.504 377 146 10 1 262 176 551 2.14e-39 145
Msa0049700 AT2G28970.2 30.504 377 146 10 1 262 141 516 2.78e-39 145
Msa0049700 AT2G19190.1 32.143 364 143 12 1 262 284 645 7.61e-39 144
Msa0049700 AT3G21340.2 29.610 385 146 10 1 262 282 664 2.37e-38 142
Msa0049700 AT3G21340.1 29.610 385 146 10 1 262 282 664 3.27e-38 142
Msa0049700 AT1G51800.1 28.198 383 153 10 1 262 276 657 3.64e-37 139
Msa0049700 AT4G20450.2 29.268 369 153 9 1 262 296 663 5.79e-37 139
Msa0049700 AT4G20450.1 29.268 369 153 9 1 262 296 663 7.14e-37 138
Msa0049700 AT1G49100.1 28.947 380 147 10 1 262 279 653 4.16e-36 136
Msa0049700 AT3G46330.2 30.267 337 159 9 1 262 279 614 4.45e-36 136
Msa0049700 AT2G14440.1 29.640 361 149 9 1 262 281 635 1.23e-35 135
Msa0049700 AT5G59680.1 30.971 381 136 11 2 262 279 652 1.45e-35 134
Msa0049700 AT2G28990.2 27.778 378 156 7 1 262 273 649 3.11e-35 134
Msa0049700 AT2G28990.1 27.778 378 156 7 1 262 273 649 3.11e-35 134
Msa0049700 AT3G46400.1 28.108 370 156 9 2 262 280 648 6.90e-35 132
Msa0049700 AT2G14510.1 29.363 361 150 9 1 262 280 634 8.63e-35 132
Msa0049700 AT2G14510.2 29.363 361 150 9 1 262 288 642 8.66e-35 132
Msa0049700 AT5G16900.4 28.342 374 156 9 1 262 86 459 1.56e-34 131
Msa0049700 AT5G16900.1 28.342 374 156 9 1 262 272 645 3.56e-34 130
Msa0049700 AT5G16900.3 28.342 374 156 9 1 262 272 645 3.56e-34 130
Msa0049700 AT3G46370.2 29.144 374 147 9 2 262 191 559 4.62e-34 130
Msa0049700 AT3G46370.1 29.144 374 147 9 2 262 191 559 4.62e-34 130
Msa0049700 AT2G29000.1 31.250 368 143 11 1 262 274 637 7.26e-34 130
Msa0049700 AT3G46330.1 29.363 361 155 11 1 262 279 638 7.49e-34 130
Msa0049700 AT1G07550.1 29.917 361 147 13 1 262 278 631 1.95e-32 125
Msa0049700 AT1G51790.3 26.984 378 150 11 1 262 281 648 9.36e-32 124
Msa0049700 AT1G51790.1 26.984 378 150 11 1 262 281 648 9.36e-32 124
Msa0049700 AT4G29180.2 29.973 377 140 16 1 262 285 652 6.16e-29 115
Msa0049700 AT1G05700.2 32.959 267 94 7 1 188 280 540 1.21e-28 114
Msa0049700 AT1G05700.2 50.000 72 35 1 191 262 602 672 1.98e-14 73.2
Msa0049700 AT4G29180.3 29.947 374 141 16 1 262 285 649 3.09e-28 113
Msa0049700 AT5G59650.1 29.867 375 147 13 2 262 279 651 3.09e-28 113
Msa0049700 AT5G59660.2 32.215 298 97 7 40 238 245 536 4.85e-28 113
Msa0049700 AT5G59660.1 32.215 298 97 7 40 238 267 558 5.69e-28 112
Msa0049700 AT5G16900.2 27.147 361 151 9 1 249 272 632 2.97e-27 110
Msa0049700 AT5G59660.3 32.226 301 96 8 40 238 267 561 1.03e-26 109
Msa0049700 AT5G59650.2 28.460 383 150 13 2 262 279 659 3.79e-25 104
Msa0049700 AT2G04300.1 29.341 334 161 8 1 262 283 613 6.43e-25 103
Msa0049700 AT4G29180.1 28.571 378 142 16 1 262 285 650 1.99e-24 102
Msa0049700 AT3G46340.1 36.364 176 73 6 1 144 281 449 5.68e-22 95.5
Msa0049700 AT3G46340.1 48.649 74 37 1 190 262 585 658 6.35e-15 74.7
Msa0049700 AT2G04300.2 58.333 72 30 0 191 262 583 654 1.31e-21 94.4
Msa0049700 AT2G04300.2 36.000 175 75 5 1 144 283 451 3.35e-21 93.2
Msa0049700 AT1G51790.2 26.117 291 117 9 1 202 281 562 3.78e-20 90.1
Msa0049700 AT5G59616.1 37.226 137 56 4 2 108 17 153 3.76e-19 82.0
Msa0049700 AT5G59670.2 33.735 166 73 5 2 135 277 437 2.83e-18 84.7
Msa0049700 AT5G59670.2 49.315 73 37 0 190 262 564 636 7.46e-16 77.4
Msa0049700 AT5G59670.1 33.735 166 73 5 2 135 277 437 2.83e-18 84.7
Msa0049700 AT5G59670.1 49.315 73 37 0 190 262 564 636 7.46e-16 77.4
Msa0049700 AT2G28960.1 50.000 72 36 0 191 262 442 513 2.88e-17 81.6
Msa0049700 AT2G28960.1 35.152 165 68 6 2 134 145 302 1.20e-16 79.7
Msa0049700 AT2G28960.2 50.000 72 36 0 191 262 574 645 3.44e-17 81.3
Msa0049700 AT2G28960.2 34.940 166 69 6 2 135 277 435 1.58e-16 79.3
Msa0049700 AT5G28680.1 41.176 85 48 1 180 262 509 593 1.28e-12 67.8
Msa0049700 AT5G28680.2 41.176 85 48 1 180 262 509 593 1.28e-12 67.8
Msa0049700 AT4G29450.1 44.048 84 35 2 191 262 567 650 1.42e-12 67.8
Msa0049700 AT3G04690.1 41.176 85 48 1 180 262 505 589 1.63e-12 67.4
Msa0049700 AT2G37050.2 21.717 396 165 12 2 259 285 673 3.32e-12 66.6
Msa0049700 AT1G51620.2 47.887 71 36 1 192 262 49 118 1.68e-11 63.9
Msa0049700 AT4G32710.1 38.043 92 57 0 171 262 370 461 1.87e-11 64.3
Msa0049700 AT5G38990.1 32.824 131 80 3 140 262 468 598 2.41e-11 63.9
Msa0049700 AT4G39110.1 36.471 85 52 1 180 262 514 598 3.10e-11 63.9
Msa0049700 AT2G37050.4 37.037 81 50 1 180 259 594 674 7.66e-11 62.4

Find 59 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAACTTCCAGCGGTTTAAT+TGG 0.192363 1_2:-3168854 Msa0049700:CDS
TTTGATTTGCATCGAGCTTC+TGG 0.227717 1_2:+3170102 None:intergenic
AAATACTTGACAGGTCTTAT+AGG 0.242464 1_2:-3167130 Msa0049700:CDS
TTAATTGGAACTATAGCTTC+TGG 0.326254 1_2:-3168839 Msa0049700:CDS
AATACTTGACAGGTCTTATA+GGG 0.376188 1_2:-3167129 Msa0049700:CDS
CAATGTCGTCGAGACATATA+TGG 0.381553 1_2:-3169922 Msa0049700:CDS
GATTCATGCAACAAGAGAAA+TGG 0.383099 1_2:-3167818 Msa0049700:CDS
TACCAACATAGCTGCAGTTT+AGG 0.411492 1_2:+3168972 None:intergenic
GAAGATACAAAGGAATGAAT+AGG 0.428109 1_2:+3170033 None:intergenic
TTTGAAAAGGTTGTGGAGAA+AGG 0.428970 1_2:-3167401 Msa0049700:CDS
CAGAAACTTTGAAAAGGTTG+TGG 0.430911 1_2:-3167408 Msa0049700:CDS
ATATTCTATTGACTTCAGAT+TGG 0.437899 1_2:+3168811 None:intergenic
CCACCAACTGATACTACTAA+TGG 0.439249 1_2:+3167779 None:intergenic
CTAAGCTGCCAGTGACAACT+TGG 0.444235 1_2:-3170189 None:intergenic
TAATTGGAACTATAGCTTCT+GGG 0.444237 1_2:-3168838 Msa0049700:CDS
CCTTCAGGAACTATAGCTTC+TGG 0.445608 1_2:-3168880 Msa0049700:intron
GTGAAACGAAGTTGGAACTT+TGG 0.448803 1_2:-3169983 Msa0049700:CDS
GTTGTGGAGAAAGGAGCATC+TGG 0.469808 1_2:-3167392 Msa0049700:CDS
CTTATAGGGTATTGTGATGA+TGG 0.472954 1_2:-3167115 Msa0049700:CDS
ATTCATGCAACAAGAGAAAT+GGG 0.480576 1_2:-3167817 Msa0049700:CDS
GGCCTAAACTGCAGCTATGT+TGG 0.481031 1_2:-3168974 Msa0049700:CDS
GGCATTATACTTGGGAAGGA+AGG 0.485026 1_2:+3170160 None:intergenic
CAACAAGAGAAATGGGAGTA+AGG 0.487221 1_2:-3167810 Msa0049700:CDS
ATATGTGGAGGCATTATACT+TGG 0.487647 1_2:+3170151 None:intergenic
CTGGAACAGTCTACCATGGC+TGG 0.488403 1_2:-3167373 Msa0049700:CDS
AGTTATTAGTGAAACGAAGT+TGG 0.493413 1_2:-3169991 Msa0049700:intron
GTACTCCAGTGTCTTACTTG+TGG 0.508313 1_2:-3169000 Msa0049700:CDS
CATTACCAGAAACTTTGAAA+AGG 0.512003 1_2:-3167414 Msa0049700:intron
TCTCTTGTTGCATGAATCTG+AGG 0.513564 1_2:+3167823 None:intergenic
ACTTACAAGTATATGATCCT+TGG 0.514030 1_2:+3168941 None:intergenic
AAGTAAGACACTGGAGTACA+AGG 0.521279 1_2:+3169004 None:intergenic
ACTCTAGATTAGAGCCATGT+TGG 0.527280 1_2:+3167090 None:intergenic
GTTCATCATAAATACTTGAC+AGG 0.530148 1_2:-3167139 Msa0049700:CDS
CCAGTGTCTTACTTGTGGAA+TGG 0.545016 1_2:-3168995 Msa0049700:CDS
ATAGAAAGCCAAACCTACCT+TGG 0.547821 1_2:+3167699 None:intergenic
TATAGTTCCAATTAAACCGC+TGG 0.550918 1_2:+3168847 None:intergenic
GTCTCGACGACATTGAATAG+TGG 0.551109 1_2:+3169930 None:intergenic
GTTCCATTAGTAGTATCAGT+TGG 0.554832 1_2:-3167782 Msa0049700:CDS
CAGATATCCTTGAGTTGATG+AGG 0.561128 1_2:+3167310 None:intergenic
GTGCATGTACACATAATATG+TGG 0.566614 1_2:+3170136 None:intergenic
CCATTAGTAGTATCAGTTGG+TGG 0.569905 1_2:-3167779 Msa0049700:CDS
TTCTGGAACTTAACTTCCAG+CGG 0.576842 1_2:-3168863 Msa0049700:CDS
CTATCTTCCTCATCAACTCA+AGG 0.579253 1_2:-3167317 Msa0049700:CDS
TGGAGGCATTATACTTGGGA+AGG 0.584867 1_2:+3170156 None:intergenic
AAACTGCAGCTATGTTGGTA+CGG 0.585202 1_2:-3168969 Msa0049700:CDS
TACAGGTTCTGAAGATACAA+AGG 0.605212 1_2:+3170023 None:intergenic
CCATTCCACAAGTAAGACAC+TGG 0.617935 1_2:+3168995 None:intergenic
CGTCGAGACATATATGGCAA+AGG 0.622385 1_2:-3169916 Msa0049700:CDS
TATGTGGAGGCATTATACTT+GGG 0.630511 1_2:+3170152 None:intergenic
TATCATGATCAATCCAGCCA+TGG 0.635701 1_2:+3167360 None:intergenic
TCGACGACATTGAATAGTGG+TGG 0.637303 1_2:+3169933 None:intergenic
ATATCTGCAATTTCAAGCAG+AGG 0.641697 1_2:-3167294 Msa0049700:intron
CATGTACACATAATATGTGG+AGG 0.657421 1_2:+3170139 None:intergenic
GCATCTGGAACAGTCTACCA+TGG 0.666857 1_2:-3167377 Msa0049700:CDS
ATGTTGGTACGGATTCGCCA+AGG 0.677061 1_2:-3168958 Msa0049700:CDS
ACTACAAGAGACACCAAGGT+AGG 0.680841 1_2:-3167712 Msa0049700:intron
CTGGATTGATCATGATACTG+AGG 0.708911 1_2:-3167354 Msa0049700:CDS
TTGTGATGATGGCACCAACA+TGG 0.719558 1_2:-3167104 Msa0049700:CDS
ATATACTACAAGAGACACCA+AGG 0.741720 1_2:-3167716 Msa0049700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATATTTTTATTAAA+TGG - chr1_2:3167680-3167699 Msa0049700:intron 0.0%
!!! AAAATAAAAAAACAGTTTTT+TGG - chr1_2:3168016-3168035 Msa0049700:intron 10.0%
!! TAAAAAAATTGAAAAAATAC+TGG + chr1_2:3168133-3168152 None:intergenic 10.0%
!!! TTTTATTTCTTCTTTATATT+TGG - chr1_2:3169155-3169174 Msa0049700:intron 10.0%
!! ATTTATATATCAAAAATGTA+TGG - chr1_2:3169349-3169368 Msa0049700:intron 10.0%
!! ATAAAAATAGATGATTTAAT+TGG - chr1_2:3169629-3169648 Msa0049700:intron 10.0%
!! TAAAAATAGATGATTTAATT+GGG - chr1_2:3169630-3169649 Msa0049700:intron 10.0%
!! TAATAACTGATATATAATAC+AGG + chr1_2:3167270-3167289 None:intergenic 15.0%
!! TTTAAAAATAAAATCTGATG+TGG + chr1_2:3167726-3167745 None:intergenic 15.0%
!!! ATTTTTCTACTAAAAAAATC+TGG + chr1_2:3167995-3168014 None:intergenic 15.0%
!!! TCTATTTTTATTCTATTGTT+CGG + chr1_2:3169620-3169639 None:intergenic 15.0%
!! ATAATTTGACAATTTATGAT+CGG - chr1_2:3169758-3169777 Msa0049700:intron 15.0%
!!! AGTTCAACATTTTTGTAAAT+GGG - chr1_2:3167200-3167219 Msa0049700:intron 20.0%
!! TCTGAAAAAAAAAATTTACG+AGG + chr1_2:3167603-3167622 None:intergenic 20.0%
!! TTGAAAAAATACTGGAATTT+AGG + chr1_2:3168125-3168144 None:intergenic 20.0%
!! ATACTGGAATTTAAAAATAC+TGG + chr1_2:3168165-3168184 None:intergenic 20.0%
!! TCAATTAATTATATTGCTTG+AGG - chr1_2:3168681-3168700 Msa0049700:intron 20.0%
!!! TTTTATTTACTGAAGAAACT+TGG - chr1_2:3168756-3168775 Msa0049700:intron 20.0%
!! AAAACTATACAAATTATGCA+AGG + chr1_2:3168859-3168878 None:intergenic 20.0%
!! TAGCCAAGTAATATATTAAT+GGG + chr1_2:3168895-3168914 None:intergenic 20.0%
!! AACTTTAAATAGAACATACT+TGG + chr1_2:3169274-3169293 None:intergenic 20.0%
!! GAGTTCAACATTTTTGTAAA+TGG - chr1_2:3167199-3167218 Msa0049700:intron 25.0%
! AACTAAATAGTACATACCAT+CGG + chr1_2:3167410-3167429 None:intergenic 25.0%
! TTCTTACAATCTTATTCGTA+TGG - chr1_2:3167506-3167525 Msa0049700:intron 25.0%
! TCTTACAATCTTATTCGTAT+GGG - chr1_2:3167507-3167526 Msa0049700:intron 25.0%
! GGTAAGACATTTAAAAATAC+TGG + chr1_2:3168104-3168123 None:intergenic 25.0%
! AAGGAAAACATTGAAAATAC+TGG + chr1_2:3168181-3168200 None:intergenic 25.0%
! TAGCATCAACTATTATCATA+AGG + chr1_2:3168200-3168219 None:intergenic 25.0%
! TAAATGTGAAGTCAACTTAT+GGG - chr1_2:3168223-3168242 Msa0049700:intron 25.0%
! TCATATACTTGTAAGTTATG+TGG - chr1_2:3168339-3168358 Msa0049700:intron 25.0%
!!! ATATTCTATTGACTTCAGAT+TGG + chr1_2:3168465-3168484 None:intergenic 25.0%
! GATCACAAAATAGAATGTTA+AGG - chr1_2:3168541-3168560 Msa0049700:intron 25.0%
!!! CAAGGTTTATTTTTCTTTTG+GGG + chr1_2:3168841-3168860 None:intergenic 25.0%
!!! GCAAGGTTTATTTTTCTTTT+GGG + chr1_2:3168842-3168861 None:intergenic 25.0%
!!! TGCAAGGTTTATTTTTCTTT+TGG + chr1_2:3168843-3168862 None:intergenic 25.0%
! TACCCCATTAATATATTACT+TGG - chr1_2:3168889-3168908 Msa0049700:intron 25.0%
! AGCCAAGTAATATATTAATG+GGG + chr1_2:3168894-3168913 None:intergenic 25.0%
! GTAGCCAAGTAATATATTAA+TGG + chr1_2:3168896-3168915 None:intergenic 25.0%
!! TTGTCGCTTCATTAAATATA+AGG - chr1_2:3169069-3169088 Msa0049700:intron 25.0%
!! AGCTAATACAATTTTTACGA+GGG + chr1_2:3169183-3169202 None:intergenic 25.0%
!! AAGCTAATACAATTTTTACG+AGG + chr1_2:3169184-3169203 None:intergenic 25.0%
!!! GTGTGATTTTTAAGATAATC+AGG - chr1_2:3169713-3169732 Msa0049700:intron 25.0%
! ACAATTTATGATCGGTAAAT+TGG - chr1_2:3169766-3169785 Msa0049700:intron 25.0%
GAAGATACAAAGGAATGAAT+AGG + chr1_2:3167243-3167262 None:intergenic 30.0%
AGTTATTAGTGAAACGAAGT+TGG - chr1_2:3167282-3167301 Msa0049700:intron 30.0%
TCAAAAACATATCAAACCGA+TGG - chr1_2:3167391-3167410 Msa0049700:CDS 30.0%
CATGTAAATAATGAAGTGCA+TGG + chr1_2:3167481-3167500 None:intergenic 30.0%
CATGCACTTCATTATTTACA+TGG - chr1_2:3167479-3167498 Msa0049700:intron 30.0%
ACCAAACCGAAAATTTCTTA+GGG + chr1_2:3167574-3167593 None:intergenic 30.0%
AACCAAACCGAAAATTTCTT+AGG + chr1_2:3167575-3167594 None:intergenic 30.0%
TGGAAAATTAATTTCACACG+TGG + chr1_2:3167706-3167725 None:intergenic 30.0%
! CATAAGTTGACTTCACATTT+AGG + chr1_2:3168224-3168243 None:intergenic 30.0%
CTAAATGTGAAGTCAACTTA+TGG - chr1_2:3168222-3168241 Msa0049700:intron 30.0%
ACTTACAAGTATATGATCCT+TGG + chr1_2:3168335-3168354 None:intergenic 30.0%
TTAATTGGAACTATAGCTTC+TGG - chr1_2:3168434-3168453 Msa0049700:intron 30.0%
TAATTGGAACTATAGCTTCT+GGG - chr1_2:3168435-3168454 Msa0049700:intron 30.0%
TTGTCGAATAACAATTTGAC+TGG - chr1_2:3168566-3168585 Msa0049700:intron 30.0%
ATCTTCATCTAAGTTATCCT+AGG - chr1_2:3168645-3168664 Msa0049700:intron 30.0%
ATTTACTGAAGAAACTTGGA+AGG - chr1_2:3168760-3168779 Msa0049700:intron 30.0%
! GCTACTCTAAACAATTTTGT+TGG - chr1_2:3168911-3168930 Msa0049700:intron 30.0%
! CTAATACAATTTTTACGAGG+GGG + chr1_2:3169181-3169200 None:intergenic 30.0%
! GCTAATACAATTTTTACGAG+GGG + chr1_2:3169182-3169201 None:intergenic 30.0%
! ATTACTGTATGTGCAAGTTT+TGG - chr1_2:3169407-3169426 Msa0049700:intron 30.0%
ATTCATGCAACAAGAGAAAT+GGG - chr1_2:3169456-3169475 Msa0049700:intron 30.0%
! CATTACCAGAAACTTTGAAA+AGG - chr1_2:3169859-3169878 Msa0049700:intron 30.0%
ATGAACTACAGCAAAAAGTT+TGG + chr1_2:3170120-3170139 None:intergenic 30.0%
GTTCATCATAAATACTTGAC+AGG - chr1_2:3170134-3170153 Msa0049700:exon 30.0%
! AAATACTTGACAGGTCTTAT+AGG - chr1_2:3170143-3170162 Msa0049700:five_prime_UTR 30.0%
! AATACTTGACAGGTCTTATA+GGG - chr1_2:3170144-3170163 Msa0049700:five_prime_UTR 30.0%
!! TATGTGGAGGCATTATACTT+GGG + chr1_2:3167124-3167143 None:intergenic 35.0%
!! ATATGTGGAGGCATTATACT+TGG + chr1_2:3167125-3167144 None:intergenic 35.0%
CATGTACACATAATATGTGG+AGG + chr1_2:3167137-3167156 None:intergenic 35.0%
GTGCATGTACACATAATATG+TGG + chr1_2:3167140-3167159 None:intergenic 35.0%
!! TACAGGTTCTGAAGATACAA+AGG + chr1_2:3167253-3167272 None:intergenic 35.0%
! TTCCTTTTGTAGTCATTTCC+AGG - chr1_2:3167542-3167561 Msa0049700:intron 35.0%
AACCTGGAAATGACTACAAA+AGG + chr1_2:3167547-3167566 None:intergenic 35.0%
AAACCGAAAATTTCTTAGGG+GGG + chr1_2:3167571-3167590 None:intergenic 35.0%
CAAACCGAAAATTTCTTAGG+GGG + chr1_2:3167572-3167591 None:intergenic 35.0%
CCAAACCGAAAATTTCTTAG+GGG + chr1_2:3167573-3167592 None:intergenic 35.0%
!! TACATTTTGCTGAGTTGGAT+GGG + chr1_2:3167638-3167657 None:intergenic 35.0%
!! TTACATTTTGCTGAGTTGGA+TGG + chr1_2:3167639-3167658 None:intergenic 35.0%
! CGTATTACATTTTGCTGAGT+TGG + chr1_2:3167643-3167662 None:intergenic 35.0%
ACTCAGCAAAATGTAATACG+TGG - chr1_2:3167643-3167662 Msa0049700:intron 35.0%
AGTTCCTGAAGGATTATCAT+CGG + chr1_2:3168385-3168404 None:intergenic 35.0%
TTAACTTCCAGCGGTTTAAT+TGG - chr1_2:3168419-3168438 Msa0049700:intron 35.0%
TATAGTTCCAATTAAACCGC+TGG + chr1_2:3168429-3168448 None:intergenic 35.0%
TTGAATAATCAAGTCAGCCT+AGG + chr1_2:3168665-3168684 None:intergenic 35.0%
TACTATTCTTTGAACGCACA+AGG + chr1_2:3169242-3169261 None:intergenic 35.0%
ACTAACACATCACGTACAAA+AGG + chr1_2:3169319-3169338 None:intergenic 35.0%
GATTCATGCAACAAGAGAAA+TGG - chr1_2:3169455-3169474 Msa0049700:intron 35.0%
GTTCCATTAGTAGTATCAGT+TGG - chr1_2:3169491-3169510 Msa0049700:intron 35.0%
ATATACTACAAGAGACACCA+AGG - chr1_2:3169557-3169576 Msa0049700:intron 35.0%
GCTTTCTATTCCATTGACAT+TGG - chr1_2:3169588-3169607 Msa0049700:intron 35.0%
TCTATTCCATTGACATTGGT+AGG - chr1_2:3169592-3169611 Msa0049700:intron 35.0%
! CACAACCTTTTCAAAGTTTC+TGG + chr1_2:3169867-3169886 None:intergenic 35.0%
! CAGAAACTTTGAAAAGGTTG+TGG - chr1_2:3169865-3169884 Msa0049700:intron 35.0%
TTTGAAAAGGTTGTGGAGAA+AGG - chr1_2:3169872-3169891 Msa0049700:intron 35.0%
ATATCTGCAATTTCAAGCAG+AGG - chr1_2:3169979-3169998 Msa0049700:CDS 35.0%
CTTGTGAACTGAAGGAATTA+TGG - chr1_2:3170073-3170092 Msa0049700:CDS 35.0%
!! CTTATAGGGTATTGTGATGA+TGG - chr1_2:3170158-3170177 Msa0049700:five_prime_UTR 35.0%
!! TTTGATTTGCATCGAGCTTC+TGG + chr1_2:3167174-3167193 None:intergenic 40.0%
GTGAAACGAAGTTGGAACTT+TGG - chr1_2:3167290-3167309 Msa0049700:intron 40.0%
CAATGTCGTCGAGACATATA+TGG - chr1_2:3167351-3167370 Msa0049700:CDS 40.0%
! TTCCCCCCCTAAGAAATTTT+CGG - chr1_2:3167565-3167584 Msa0049700:intron 40.0%
AACCGAAAATTTCTTAGGGG+GGG + chr1_2:3167570-3167589 None:intergenic 40.0%
! CCCCTAAGAAATTTTCGGTT+TGG - chr1_2:3167570-3167589 Msa0049700:intron 40.0%
!! ACATTTTGCTGAGTTGGATG+GGG + chr1_2:3167637-3167656 None:intergenic 40.0%
CTTATGGGATCAAGAGAAAC+TGG - chr1_2:3168238-3168257 Msa0049700:intron 40.0%
AAGTAAGACACTGGAGTACA+AGG + chr1_2:3168272-3168291 None:intergenic 40.0%
TACCAACATAGCTGCAGTTT+AGG + chr1_2:3168304-3168323 None:intergenic 40.0%
AAACTGCAGCTATGTTGGTA+CGG - chr1_2:3168304-3168323 Msa0049700:intron 40.0%
!! TTCTGGAACTTAACTTCCAG+CGG - chr1_2:3168410-3168429 Msa0049700:intron 40.0%
AAGGAAATCAGCTATCAGCA+GGG - chr1_2:3168779-3168798 Msa0049700:intron 40.0%
GCGAATCCCTTCATAACAAT+GGG - chr1_2:3168802-3168821 Msa0049700:intron 40.0%
CGAATCCCTTCATAACAATG+GGG - chr1_2:3168803-3168822 Msa0049700:intron 40.0%
TGCTACCCCATTGTTATGAA+GGG + chr1_2:3168811-3168830 None:intergenic 40.0%
ATGCTACCCCATTGTTATGA+AGG + chr1_2:3168812-3168831 None:intergenic 40.0%
TCATAACAATGGGGTAGCAT+TGG - chr1_2:3168812-3168831 Msa0049700:CDS 40.0%
TCTCTTGTTGCATGAATCTG+AGG + chr1_2:3169453-3169472 None:intergenic 40.0%
CAACAAGAGAAATGGGAGTA+AGG - chr1_2:3169463-3169482 Msa0049700:intron 40.0%
CCACCAACTGATACTACTAA+TGG + chr1_2:3169497-3169516 None:intergenic 40.0%
CCATTAGTAGTATCAGTTGG+TGG - chr1_2:3169494-3169513 Msa0049700:intron 40.0%
ATAGAAAGCCAAACCTACCT+TGG + chr1_2:3169577-3169596 None:intergenic 40.0%
TATCATGATCAATCCAGCCA+TGG + chr1_2:3169916-3169935 None:intergenic 40.0%
CTGGATTGATCATGATACTG+AGG - chr1_2:3169919-3169938 Msa0049700:CDS 40.0%
CTATCTTCCTCATCAACTCA+AGG - chr1_2:3169956-3169975 Msa0049700:CDS 40.0%
!! CAGATATCCTTGAGTTGATG+AGG + chr1_2:3169966-3169985 None:intergenic 40.0%
ACGATGTTCTTGTGAACTGA+AGG - chr1_2:3170065-3170084 Msa0049700:CDS 40.0%
! GGTTTTCCTTTCTGATTCAC+AGG - chr1_2:3170094-3170113 Msa0049700:CDS 40.0%
GGCATTATACTTGGGAAGGA+AGG + chr1_2:3167116-3167135 None:intergenic 45.0%
!! TGGAGGCATTATACTTGGGA+AGG + chr1_2:3167120-3167139 None:intergenic 45.0%
TCGACGACATTGAATAGTGG+TGG + chr1_2:3167343-3167362 None:intergenic 45.0%
GTCTCGACGACATTGAATAG+TGG + chr1_2:3167346-3167365 None:intergenic 45.0%
CGTCGAGACATATATGGCAA+AGG - chr1_2:3167357-3167376 Msa0049700:CDS 45.0%
!! CATTTTGCTGAGTTGGATGG+GGG + chr1_2:3167636-3167655 None:intergenic 45.0%
!! TGTAATACGTGGCGCTGATT+TGG - chr1_2:3167654-3167673 Msa0049700:intron 45.0%
GTACTCCAGTGTCTTACTTG+TGG - chr1_2:3168273-3168292 Msa0049700:intron 45.0%
CCATTCCACAAGTAAGACAC+TGG + chr1_2:3168281-3168300 None:intergenic 45.0%
CCAGTGTCTTACTTGTGGAA+TGG - chr1_2:3168278-3168297 Msa0049700:intron 45.0%
TACGCCGATGATAATCCTTC+AGG - chr1_2:3168378-3168397 Msa0049700:intron 45.0%
CCAGAAGCTATAGTTCCTGA+AGG + chr1_2:3168396-3168415 None:intergenic 45.0%
CCTTCAGGAACTATAGCTTC+TGG - chr1_2:3168393-3168412 Msa0049700:intron 45.0%
CGTAGCTGAGATAGGAAATC+AGG + chr1_2:3168599-3168618 None:intergenic 45.0%
! TCAAGAAGCGTAGCTGAGAT+AGG + chr1_2:3168607-3168626 None:intergenic 45.0%
GAAGGAAATCAGCTATCAGC+AGG - chr1_2:3168778-3168797 Msa0049700:intron 45.0%
AGGAAATCAGCTATCAGCAG+GGG - chr1_2:3168780-3168799 Msa0049700:intron 45.0%
GGCGAATCCCTTCATAACAA+TGG - chr1_2:3168801-3168820 Msa0049700:intron 45.0%
! AATTGTATTAGCTTCGCCGC+TGG - chr1_2:3169190-3169209 Msa0049700:intron 45.0%
GAATTGATCCTGATAGCCAG+CGG + chr1_2:3169209-3169228 None:intergenic 45.0%
GAACGCACAAGGAGTTGTAT+TGG + chr1_2:3169231-3169250 None:intergenic 45.0%
ACTACAAGAGACACCAAGGT+AGG - chr1_2:3169561-3169580 Msa0049700:intron 45.0%
AAGAGACACCAAGGTAGGTT+TGG - chr1_2:3169566-3169585 Msa0049700:intron 45.0%
GTTTGGCCTGTGAATCAGAA+AGG + chr1_2:3170103-3170122 None:intergenic 45.0%
!! TTGTGATGATGGCACCAACA+TGG - chr1_2:3170169-3170188 Msa0049700:five_prime_UTR 45.0%
!!! TTTAATATTTTTTTAGTTTT+GGG + chr1_2:3167783-3167802 None:intergenic 5.0%
!!! TTTTAATATTTTTTTAGTTT+TGG + chr1_2:3167784-3167803 None:intergenic 5.0%
AATTTCTTAGGGGGGGAACC+TGG + chr1_2:3167563-3167582 None:intergenic 50.0%
TGGGATCAAGAGAAACTGGC+AGG - chr1_2:3168242-3168261 Msa0049700:intron 50.0%
GGCCTAAACTGCAGCTATGT+TGG - chr1_2:3168299-3168318 Msa0049700:intron 50.0%
ATGTTGGTACGGATTCGCCA+AGG - chr1_2:3168315-3168334 Msa0049700:intron 50.0%
TCGGCTCCTACCAATGTCAA+TGG + chr1_2:3169601-3169620 None:intergenic 50.0%
!! GTTGTGGAGAAAGGAGCATC+TGG - chr1_2:3169881-3169900 Msa0049700:intron 50.0%
! GCATCTGGAACAGTCTACCA+TGG - chr1_2:3169896-3169915 Msa0049700:CDS 50.0%
! TTGCTGAGTTGGATGGGGGA+GGG + chr1_2:3167632-3167651 None:intergenic 55.0%
! TTTGCTGAGTTGGATGGGGG+AGG + chr1_2:3167633-3167652 None:intergenic 55.0%
CTGGAACAGTCTACCATGGC+TGG - chr1_2:3169900-3169919 Msa0049700:CDS 55.0%
! TGCTGAGTTGGATGGGGGAG+GGG + chr1_2:3167631-3167650 None:intergenic 60.0%
! TAGCTTCGCCGCTGGCTATC+AGG - chr1_2:3169198-3169217 Msa0049700:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 3167096 3170199 3167096 ID=Msa0049700;Name=Msa0049700
chr1_2 mRNA 3167096 3170199 3167096 ID=Msa0049700-mRNA-1;Parent=Msa0049700;Name=Msa0049700-mRNA-1;_AED=0.21;_eAED=0.29;_QI=58|0|0|1|0.16|0|7|0|262
chr1_2 exon 3170026 3170199 3170026 ID=Msa0049700-mRNA-1:exon:756;Parent=Msa0049700-mRNA-1
chr1_2 exon 3169883 3170004 3169883 ID=Msa0049700-mRNA-1:exon:755;Parent=Msa0049700-mRNA-1
chr1_2 exon 3168947 3169031 3168947 ID=Msa0049700-mRNA-1:exon:754;Parent=Msa0049700-mRNA-1
chr1_2 exon 3168809 3168895 3168809 ID=Msa0049700-mRNA-1:exon:753;Parent=Msa0049700-mRNA-1
chr1_2 exon 3167713 3167871 3167713 ID=Msa0049700-mRNA-1:exon:752;Parent=Msa0049700-mRNA-1
chr1_2 exon 3167295 3167427 3167295 ID=Msa0049700-mRNA-1:exon:751;Parent=Msa0049700-mRNA-1
chr1_2 exon 3167096 3167179 3167096 ID=Msa0049700-mRNA-1:exon:750;Parent=Msa0049700-mRNA-1
chr1_2 five_prime_UTR 3170142 3170199 3170142 ID=Msa0049700-mRNA-1:five_prime_utr;Parent=Msa0049700-mRNA-1
chr1_2 CDS 3170026 3170141 3170026 ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2 CDS 3169883 3170004 3169883 ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2 CDS 3168947 3169031 3168947 ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2 CDS 3168809 3168895 3168809 ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2 CDS 3167713 3167871 3167713 ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2 CDS 3167295 3167427 3167295 ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2 CDS 3167096 3167179 3167096 ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
Gene Sequence

>Msa0049700

ATGCACTTTGCTGAAATCCAGAAGCTCGATGCAAATCAAATTAGAGAGTTCAACATTTTTGTAAATGGGCAGCTTATAAATAGTGACCTATTCATTCCTTTGTATCTTCAGAACCTTGAAACGAAGTTGGAACTTTGGTTTAATAAGACTAGTAGATCAACTCTTCCACCACTATTCAATGTCGTCGAGACATATATGGCAAAGGACTTCTTACAATCAAAAACATATCAAACCGATGGAGATCCTTGTACTCCAGTGTCTTACTTGTGGAATGGCCTAAACTGCAGCTATGTTGGTACGGATTCGCCAAGGATCATATACTTGAACTATAGCTTCTGGAACTTAACTTCCAGCGGTTTAATTGGAACTATAGCTTCTGGGATATCCAATCTGAAGTCAATAGAATATTTTTTTGGTAGAAATTCAAATCTTTGCACCTCAGATTCATGCAACAAGAGAAATGGGAGTAAGGTTTTAGTTCCATTAGTAGTATCAGTTGGTGGAGCTTTCATTATACTAGTAGTAGCAATGATTTCTTTTCGCATATACTACAAGAGACACCAAGGTAGAAACTTTGAAAAGGTTGTGGAGAAAGGAGCATCTGGAACAGTCTACCATGGCTGGATTGATCATGATACTGAGGTAGCTGTCAAAATGCTATCTTCCTCATCAACTCAAGGATATCTGCAATTTCAAGCAGAGGCCAAACTTTTTGCTGTAGTTCATCATAAATACTTGACAGGTCTTATAGGGTATTGTGATGATGGCACCAACATGGCTCTAATC
Protein sequence

>Msa0049700

MHFAEIQKLDANQIREFNIFVNGQLINSDLFIPLYLQNLETKLELWFNKTSRSTLPPLFNVVETYMAKDFLQSKTYQTDGDPCTPVSYLWNGLNCSYVGTDSPRIIYLNYSFWNLTSSGLIGTIASGISNLKSIEYFFGRNSNLCTSDSCNKRNGSKVLVPLVVSVGGAFIILVVAMISFRIYYKRHQGRNFEKVVEKGASGTVYHGWIDHDTEVAVKMLSSSSTQGYLQFQAEAKLFAVVHHKYLTGLIGYCDDGTNMALI