AlfalfaGEDB | Msa0049700

M. sativa cultivar zhongmu-4 / Msa0049700

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0098542
PFAM: PF12819.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049700	sp\|O65924\|Y2921_ARATH	36.388	371	120	10	1	262	284	647	2.18e-55	194

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049700	G7IHW2	62.155	362	32	5	1	262	268	624	8.40e-135	407

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049700	MtrunA17_Chr2g0330591	56.873	371	45	6	1	262	282	646	3.63e-125	376
Msa0049700	MtrunA17_Chr2g0330631	71.519	158	16	1	134	262	2	159	2.04e-72	222
Msa0049700	MtrunA17_Chr2g0330621	71.875	160	15	3	1	135	49	203	5.14e-71	216
Msa0049700	MtrunA17_Chr8g0341011	33.871	372	126	12	1	262	281	642	2.26e-49	174
Msa0049700	MtrunA17_Chr8g0341151	33.151	365	131	9	2	262	277	632	2.45e-49	173
Msa0049700	MtrunA17_Chr8g0340931	33.784	370	127	11	1	262	280	639	2.90e-49	173
Msa0049700	MtrunA17_Chr8g0340891	32.329	365	133	8	1	262	284	637	1.87e-48	171
Msa0049700	MtrunA17_Chr8g0340911	33.333	366	132	9	1	262	282	639	3.79e-48	170
Msa0049700	MtrunA17_Chr8g0340871	31.903	373	133	9	1	262	286	648	3.13e-46	165
Msa0049700	MtrunA17_Chr8g0347661	31.608	367	136	9	1	262	288	644	4.46e-45	161

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049700	AT2G19210.1	36.388	371	120	10	1	262	284	647	2.21e-56	194
Msa0049700	AT1G51880.2	36.585	369	119	9	1	262	295	655	2.04e-53	186
Msa0049700	AT1G51880.1	36.585	369	119	9	1	262	277	637	2.07e-53	185
Msa0049700	AT1G51870.1	36.527	334	131	7	1	262	278	602	3.68e-53	184
Msa0049700	AT2G19230.2	34.540	359	136	11	1	262	284	640	1.73e-52	183
Msa0049700	AT1G51880.3	35.809	377	119	9	1	262	295	663	2.46e-52	182
Msa0049700	AT2G19230.1	34.444	360	136	11	1	262	284	641	4.02e-52	182
Msa0049700	AT1G51805.2	32.134	389	127	9	1	262	247	625	7.93e-51	178
Msa0049700	AT1G51805.1	32.134	389	127	9	1	262	271	649	8.31e-51	178
Msa0049700	AT1G51890.5	32.973	370	139	6	1	262	1	369	5.31e-50	173

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Find 59 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTGCAGCTATGTTGGTA+CGG	0.585202	1_2:-3168969	Msa0049700:CDS
AAATACTTGACAGGTCTTAT+AGG	0.242464	1_2:-3167130	Msa0049700:CDS
AAGTAAGACACTGGAGTACA+AGG	0.521279	1_2:+3169004	None:intergenic
AATACTTGACAGGTCTTATA+GGG	0.376188	1_2:-3167129	Msa0049700:CDS
ACTACAAGAGACACCAAGGT+AGG	0.680841	1_2:-3167712	Msa0049700:intron
ACTCTAGATTAGAGCCATGT+TGG	0.527280	1_2:+3167090	None:intergenic
ACTTACAAGTATATGATCCT+TGG	0.514030	1_2:+3168941	None:intergenic
AGTTATTAGTGAAACGAAGT+TGG	0.493413	1_2:-3169991	Msa0049700:intron
ATAGAAAGCCAAACCTACCT+TGG	0.547821	1_2:+3167699	None:intergenic
ATATACTACAAGAGACACCA+AGG	0.741720	1_2:-3167716	Msa0049700:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAGATACAAAGGAATGAAT+AGG	+	chr1_2:3167243-3167262	None:intergenic	30.0%
AGTTATTAGTGAAACGAAGT+TGG	-	chr1_2:3167282-3167301	Msa0049700:intron	30.0%
TCAAAAACATATCAAACCGA+TGG	-	chr1_2:3167391-3167410	Msa0049700:CDS	30.0%
CATGTAAATAATGAAGTGCA+TGG	+	chr1_2:3167481-3167500	None:intergenic	30.0%
CATGCACTTCATTATTTACA+TGG	-	chr1_2:3167479-3167498	Msa0049700:intron	30.0%
ACCAAACCGAAAATTTCTTA+GGG	+	chr1_2:3167574-3167593	None:intergenic	30.0%
AACCAAACCGAAAATTTCTT+AGG	+	chr1_2:3167575-3167594	None:intergenic	30.0%
TGGAAAATTAATTTCACACG+TGG	+	chr1_2:3167706-3167725	None:intergenic	30.0%
CTAAATGTGAAGTCAACTTA+TGG	-	chr1_2:3168222-3168241	Msa0049700:intron	30.0%
ACTTACAAGTATATGATCCT+TGG	+	chr1_2:3168335-3168354	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	3167096	3170199	3167096	ID=Msa0049700;Name=Msa0049700
chr1_2	mRNA	3167096	3170199	3167096	ID=Msa0049700-mRNA-1;Parent=Msa0049700;Name=Msa0049700-mRNA-1;_AED=0.21;_eAED=0.29;_QI=58\|0\|0\|1\|0.16\|0\|7\|0\|262
chr1_2	exon	3170026	3170199	3170026	ID=Msa0049700-mRNA-1:exon:756;Parent=Msa0049700-mRNA-1
chr1_2	exon	3169883	3170004	3169883	ID=Msa0049700-mRNA-1:exon:755;Parent=Msa0049700-mRNA-1
chr1_2	exon	3168947	3169031	3168947	ID=Msa0049700-mRNA-1:exon:754;Parent=Msa0049700-mRNA-1
chr1_2	exon	3168809	3168895	3168809	ID=Msa0049700-mRNA-1:exon:753;Parent=Msa0049700-mRNA-1
chr1_2	exon	3167713	3167871	3167713	ID=Msa0049700-mRNA-1:exon:752;Parent=Msa0049700-mRNA-1
chr1_2	exon	3167295	3167427	3167295	ID=Msa0049700-mRNA-1:exon:751;Parent=Msa0049700-mRNA-1
chr1_2	exon	3167096	3167179	3167096	ID=Msa0049700-mRNA-1:exon:750;Parent=Msa0049700-mRNA-1
chr1_2	five_prime_UTR	3170142	3170199	3170142	ID=Msa0049700-mRNA-1:five_prime_utr;Parent=Msa0049700-mRNA-1
chr1_2	CDS	3170026	3170141	3170026	ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2	CDS	3169883	3170004	3169883	ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2	CDS	3168947	3169031	3168947	ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2	CDS	3168809	3168895	3168809	ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2	CDS	3167713	3167871	3167713	ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2	CDS	3167295	3167427	3167295	ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1
chr1_2	CDS	3167096	3167179	3167096	ID=Msa0049700-mRNA-1:cds;Parent=Msa0049700-mRNA-1

Gene Sequence

>Msa0049700

ATGCACTTTGCTGAAATCCAGAAGCTCGATGCAAATCAAATTAGAGAGTTCAACATTTTTGTAAATGGGCAGCTTATAAATAGTGACCTATTCATTCCTTTGTATCTTCAGAACCTTGAAACGAAGTTGGAACTTTGGTTTAATAAGACTAGTAGATCAACTCTTCCACCACTATTCAATGTCGTCGAGACATATATGGCAAAGGACTTCTTACAATCAAAAACATATCAAACCGATGGAGATCCTTGTACTCCAGTGTCTTACTTGTGGAATGGCCTAAACTGCAGCTATGTTGGTACGGATTCGCCAAGGATCATATACTTGAACTATAGCTTCTGGAACTTAACTTCCAGCGGTTTAATTGGAACTATAGCTTCTGGGATATCCAATCTGAAGTCAATAGAATATTTTTTTGGTAGAAATTCAAATCTTTGCACCTCAGATTCATGCAACAAGAGAAATGGGAGTAAGGTTTTAGTTCCATTAGTAGTATCAGTTGGTGGAGCTTTCATTATACTAGTAGTAGCAATGATTTCTTTTCGCATATACTACAAGAGACACCAAGGTAGAAACTTTGAAAAGGTTGTGGAGAAAGGAGCATCTGGAACAGTCTACCATGGCTGGATTGATCATGATACTGAGGTAGCTGTCAAAATGCTATCTTCCTCATCAACTCAAGGATATCTGCAATTTCAAGCAGAGGCCAAACTTTTTGCTGTAGTTCATCATAAATACTTGACAGGTCTTATAGGGTATTGTGATGATGGCACCAACATGGCTCTAATC

Protein sequence

>Msa0049700

MHFAEIQKLDANQIREFNIFVNGQLINSDLFIPLYLQNLETKLELWFNKTSRSTLPPLFNVVETYMAKDFLQSKTYQTDGDPCTPVSYLWNGLNCSYVGTDSPRIIYLNYSFWNLTSSGLIGTIASGISNLKSIEYFFGRNSNLCTSDSCNKRNGSKVLVPLVVSVGGAFIILVVAMISFRIYYKRHQGRNFEKVVEKGASGTVYHGWIDHDTEVAVKMLSSSSTQGYLQFQAEAKLFAVVHHKYLTGLIGYCDDGTNMALI