AlfalfaGEDB | Msa0051310

M. sativa cultivar zhongmu-4 / Msa0051310

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051310	XP_003589277.1	92.063	378	19	2	1	377	1	368	0.0	712

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051310	sp\|Q8RYD1\|REM16_ARATH	32.240	366	202	10	8	367	3	328	5.01e-50	174

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051310	G7I576	92.063	378	19	2	1	377	1	368	0.0	712

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TFs/TRs:

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Gene ID	Type	Classification
Msa0051310	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051310	MtrunA17_Chr1g0154161	81.217	378	17	3	1	377	1	325	0.0	611
Msa0051310	MtrunA17_Chr1g0154171	80.163	368	60	3	11	376	4	360	0.0	579
Msa0051310	MtrunA17_Chr1g0154191	68.831	385	83	8	13	377	5	372	0.0	514
Msa0051310	MtrunA17_Chr1g0154211	58.561	403	113	6	11	370	4	395	2.05e-155	443
Msa0051310	MtrunA17_Chr1g0154151	65.861	331	82	8	44	369	19	323	1.49e-144	413
Msa0051310	MtrunA17_Chr1g0154131	59.777	358	95	7	14	369	168	478	5.41e-140	407
Msa0051310	MtrunA17_Chr1g0154131	70.482	166	43	1	6	165	2	167	5.12e-73	235
Msa0051310	MtrunA17_Chr1g0154111	57.796	372	128	6	13	368	9	367	1.55e-139	409
Msa0051310	MtrunA17_Chr1g0154111	57.727	220	72	5	44	243	368	586	4.16e-69	228
Msa0051310	MtrunA17_Chr1g0154101	50.780	449	128	8	14	372	168	613	7.24e-130	392

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0051310	AT4G33280.1	32.240	366	202	10	8	367	3	328	5.09e-51	174
Msa0051310	AT4G33280.3	31.017	403	219	13	8	367	3	389	1.88e-46	164
Msa0051310	AT4G33280.2	52.555	137	63	2	8	143	3	138	9.97e-45	156
Msa0051310	AT3G18990.2	25.000	348	228	10	27	358	5	335	2.68e-20	91.7
Msa0051310	AT3G18990.1	25.000	348	228	10	27	358	5	335	2.97e-20	91.3
Msa0051310	AT4G34400.2	38.372	86	53	0	43	128	29	114	2.69e-16	80.1
Msa0051310	AT4G34400.1	38.372	86	53	0	43	128	29	114	2.69e-16	80.1
Msa0051310	AT5G66980.1	24.000	325	186	12	41	349	21	300	7.60e-14	72.4
Msa0051310	AT3G06220.1	24.731	186	111	7	30	210	11	172	2.17e-13	68.2
Msa0051310	AT3G06160.5	25.490	204	136	4	30	219	11	212	9.67e-13	68.9

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Find 73 sgRNAs with CRISPR-Local

Find 322 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGAGGTAAATGATT+CGG	0.487480	1_2:+5416079	None:intergenic
AACAAAATATGGAAAAGAGG+AGG	0.585641	1_2:+5416067	None:intergenic
AACTCCGTCCCATGTCCTAA+AGG	0.478589	1_2:-5415799	Msa0051310:CDS
AACTGAACAAGGGGATAGCA+AGG	0.567712	1_2:-5416835	Msa0051310:CDS
AACTGACTGTTTCAAATGGG+AGG	0.581709	1_2:-5419854	None:intergenic
AAGAGAAGGAGGACAAAACT+GGG	0.592018	1_2:-5419831	Msa0051310:CDS
AAGGTCCTAGTGGAGTTGTG+TGG	0.624686	1_2:-5417061	Msa0051310:CDS
ACAAGGGGATAGCAAGGCAA+AGG	0.631522	1_2:-5416829	Msa0051310:CDS
ACAGGCGTAACTTCAATAAC+AGG	0.455079	1_2:+5416681	None:intergenic
ACATGCTCAAACTGAACAAG+GGG	0.631696	1_2:-5416844	Msa0051310:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGGAAAGAATTATGAAAGA+GGG	-	chr1_2:5416255-5416274	Msa0051310:intron	30.0%
ATCATATCCTATACACCAAA+CGG	+	chr1_2:5416524-5416543	None:intergenic	30.0%
TGCCTCAAAATTGAAGAAAT+TGG	-	chr1_2:5416646-5416665	Msa0051310:CDS	30.0%
TTGTATAGAGTTTGTCTCTT+TGG	-	chr1_2:5416953-5416972	Msa0051310:CDS	30.0%
CGTTACAAACAGATTTCAAT+GGG	+	chr1_2:5417098-5417117	None:intergenic	30.0%
ACGTTACAAACAGATTTCAA+TGG	+	chr1_2:5417099-5417118	None:intergenic	30.0%
GATTGTATGATTGTGTTTGT+TGG	+	chr1_2:5417400-5417419	None:intergenic	30.0%
TTGGGAAATGAGTAGAAATT+AGG	-	chr1_2:5417537-5417556	Msa0051310:intron	30.0%
TACAATTTGATTACCCAGTT+CGG	+	chr1_2:5417680-5417699	None:intergenic	30.0%
TATCGGGGACAAATTTAAAA+TGG	+	chr1_2:5417960-5417979	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	5415618	5419861	5415618	ID=Msa0051310;Name=Msa0051310
chr1_2	mRNA	5415618	5419861	5415618	ID=Msa0051310-mRNA-1;Parent=Msa0051310;Name=Msa0051310-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0\|0\|0\|1\|0.66\|0.5\|4\|0\|377
chr1_2	exon	5419733	5419861	5419733	ID=Msa0051310-mRNA-1:exon:1640;Parent=Msa0051310-mRNA-1
chr1_2	exon	5416534	5417144	5416534	ID=Msa0051310-mRNA-1:exon:1639;Parent=Msa0051310-mRNA-1
chr1_2	exon	5416025	5416124	5416025	ID=Msa0051310-mRNA-1:exon:1638;Parent=Msa0051310-mRNA-1
chr1_2	exon	5415618	5415911	5415618	ID=Msa0051310-mRNA-1:exon:1637;Parent=Msa0051310-mRNA-1
chr1_2	CDS	5419733	5419861	5419733	ID=Msa0051310-mRNA-1:cds;Parent=Msa0051310-mRNA-1
chr1_2	CDS	5416534	5417144	5416534	ID=Msa0051310-mRNA-1:cds;Parent=Msa0051310-mRNA-1
chr1_2	CDS	5416025	5416124	5416025	ID=Msa0051310-mRNA-1:cds;Parent=Msa0051310-mRNA-1
chr1_2	CDS	5415618	5415911	5415618	ID=Msa0051310-mRNA-1:cds;Parent=Msa0051310-mRNA-1

Gene Sequence

>Msa0051310

ATGGGAGGAAGAGAAGGAGGACAAAACTGGGATGGAAGCAGATCTTGGGAGGAAGATATTTACTGGAACCATTTTCAATTCATCCATTTCACCCAATTTCTTAAAACTACAGATTTTCAACAACAACTTGCACTTCCTAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACTCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGGACTGACAACTAGAGATGATACTGTTTACTTCGTGGACAGTTGGCAACAATTCGTGAACGATCATTCTTTGAAAGAGAATGATTTCCTTGTCTTTAAGTACAATGGTGAATCACTCTTTGAGGTTTTAATCTTTGATGGAAATAGTTTTTGTGAGAAGGCAAGTTCTTATTTTGTCGGAAAATGCGGACATGCTCAAACTGAACAAGGGGATAGCAAGGCAAAGGACAATAACACTTCTGCTTTTAACGCTGGTGTTGAGTCTGCTTCCCCTCAGGTGGCTGATGCAGTCACTAAGACCACACCTGCTGCTGTTCCATCCCAAACAACCAGCAAAAGGACCAAGAAGAAGCCTGTTATTGAAGTTACGCCTGTCCAGACTAAGAAGCGAGGAAGACCACCAAAATCAGATGATTCTGGTGAGAAGCTACTACGTGATTTGGTGGCTTGTAACAAGGAACACTCAGGTTCAAATATTTCTTCCTTTTTCATTAACCTTTACTATATTTTACAAATTATTTGGAAGGAAGATGAGAAAAAAATAGCCGAATCATTTACCTCCTCTTTTCCATATTTTGTTAAAATACTTAAAGCCGGCAATGTCGGTGGTTCACGTACTCTGAGAATCCCATATCATTTCTCTGCTGCACATCTGCCTGACTTCAAAATAGAAGTTACGCTTCGCAATTCAAAAGGCGAATGTTGGACTGTGAACTCCGTCCCATGTCCTAAAGGGAAAATAATTCACTCCTTTTGTGGAGGTTGGATGGCCTTTGTTCGTGATAATGGCGTCAATTTTGGCGACACGTGCATTTTTGAGCTCGTTACTAATTATGTGATGCAGGTTTATATATTTGGGTCTGGAAAGGAAGGTGCTGACAATCAAAATGGACATGTGAAACTTGATAGCGTGTAG

Protein sequence

>Msa0051310

MGGREGGQNWDGSRSWEEDIYWNHFQFIHFTQFLKTTDFQQQLALPKTFSDNLKKKLPENVTLKGPSGVVWNIGLTTRDDTVYFVDSWQQFVNDHSLKENDFLVFKYNGESLFEVLIFDGNSFCEKASSYFVGKCGHAQTEQGDSKAKDNNTSAFNAGVESASPQVADAVTKTTPAAVPSQTTSKRTKKKPVIEVTPVQTKKRGRPPKSDDSGEKLLRDLVACNKEHSGSNISSFFINLYYILQIIWKEDEKKIAESFTSSFPYFVKILKAGNVGGSRTLRIPYHFSAAHLPDFKIEVTLRNSKGECWTVNSVPCPKGKIIHSFCGGWMAFVRDNGVNFGDTCIFELVTNYVMQVYIFGSGKEGADNQNGHVKLDSV*