AlfalfaGEDB | Msa0053300

M. sativa cultivar zhongmu-4 / Msa0053300

KEGG: K21630
GO: GO:0000003 GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001085 GO:0001228 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0040034 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0046983 GO:0048316 GO:0048367 GO:0048437 GO:0048506 GO:0048509 GO:0048510 GO:0048518 GO:0048522 GO:0048580 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000026 GO:2000112 GO:2000241 GO:2001141
PFAM: PF00320.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053300	AES59248.1	53.571	112	35	5	9	112	2	104	2.32e-19	87.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053300	sp\|Q6QPM2\|GAT19_ARATH	52.000	50	24	0	68	117	75	124	6.28e-11	60.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053300	G7I9Y7	53.571	112	35	5	9	112	2	104	1.11e-19	87.4

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TFs/TRs:

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Gene ID	Type	Classification
Msa0053300	TF	C2C2-GATA

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053300	MtrunA17_Chr1g0151471	53.571	112	35	5	9	112	2	104	2.14e-23	87.4
Msa0053300	MtrunA17_Chr1g0151461	43.158	95	45	2	27	112	140	234	9.69e-15	68.9
Msa0053300	MtrunA17_Chr7g0271861	44.068	59	33	0	68	126	22	80	2.00e-11	57.4
Msa0053300	MtrunA17_Chr2g0299081	75.000	32	8	0	66	97	167	198	8.95e-11	57.8

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053300	AT4G36620.1	52.000	50	24	68	117	75	124	6.39e-12	60.5
Msa0053300	AT5G26930.1	58.974	39	16	64	102	22	60	1.00e-11	58.2
Msa0053300	AT2G18380.1	54.000	50	23	68	117	92	141	3.14e-11	58.5

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Find 27 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTCTCTTGACACTGAAAA+GGG	0.520040	1_2:+7716258	Msa0053300:CDS
AATGGCGAATACTATATCCA+AGG	0.653439	1_2:+7715900	Msa0053300:CDS
ACCGAATACGAATACCCAAA+AGG	0.493067	1_2:+7715875	Msa0053300:CDS
ACGAATACCCAAAAGGGCAA+TGG	0.610371	1_2:+7715882	Msa0053300:CDS
ACTCCGACGAAGAAGGGAAG+AGG	0.589069	1_2:+7716224	Msa0053300:CDS
ACTGAAAAGGGAAAGGGAAA+CGG	0.566578	1_2:+7716270	Msa0053300:CDS
AGTATTCGCCATTGCCCTTT+TGG	0.253039	1_2:-7715890	None:intergenic
ATACGAACACTACTCCTCTT+TGG	0.387924	1_2:+7715978	Msa0053300:CDS
ATCGCAATCGAAACAAACCC+TGG	0.664892	1_2:-7715957	None:intergenic
ATGTGGACTCCGACGAAGAA+GGG	0.540307	1_2:+7716218	Msa0053300:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATACTATATCCAAGGAAT+TGG	+	chr1_2:7715906-7715925	Msa0053300:CDS	30.0%
AATGGCGAATACTATATCCA+AGG	+	chr1_2:7715900-7715919	Msa0053300:CDS	35.0%
TCCTAGTAAAAACACAACCA+GGG	+	chr1_2:7715940-7715959	Msa0053300:CDS	35.0%
TTCAGTGTCAAGAGACTTAT+TGG	-	chr1_2:7716256-7716275	None:intergenic	35.0%
TAAGTCTCTTGACACTGAAA+AGG	+	chr1_2:7716257-7716276	Msa0053300:CDS	35.0%
AAGTCTCTTGACACTGAAAA+GGG	+	chr1_2:7716258-7716277	Msa0053300:CDS	35.0%
ACCGAATACGAATACCCAAA+AGG	+	chr1_2:7715875-7715894	Msa0053300:CDS	40.0%
CCGAATACGAATACCCAAAA+GGG	+	chr1_2:7715876-7715895	Msa0053300:CDS	40.0%
CTCCTAGTAAAAACACAACC+AGG	+	chr1_2:7715939-7715958	Msa0053300:CDS	40.0%
ATACGAACACTACTCCTCTT+TGG	+	chr1_2:7715978-7715997	Msa0053300:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	7715758	7716329	7715758	ID=Msa0053300;Name=Msa0053300
chr1_2	mRNA	7715758	7716329	7715758	ID=Msa0053300-mRNA-1;Parent=Msa0053300;Name=Msa0053300-mRNA-1;_AED=0.10;_eAED=0.12;_QI=0\|0\|0\|1\|1\|1\|2\|0\|130
chr1_2	exon	7715758	7716024	7715758	ID=Msa0053300-mRNA-1:exon:2677;Parent=Msa0053300-mRNA-1
chr1_2	exon	7716204	7716329	7716204	ID=Msa0053300-mRNA-1:exon:2678;Parent=Msa0053300-mRNA-1
chr1_2	CDS	7715758	7716024	7715758	ID=Msa0053300-mRNA-1:cds;Parent=Msa0053300-mRNA-1
chr1_2	CDS	7716204	7716329	7716204	ID=Msa0053300-mRNA-1:cds;Parent=Msa0053300-mRNA-1

Gene Sequence

>Msa0053300

ATGAGAGTAAGTAACGATGATCCAGTTCCAGCCACAAAGAAGAAAAAAACTGCTGCTAGTGCTGCCAAAAAGAAGAAATCAGTTGCTAGTGCTTCCAAAAAGAAAATAAAAACTGCTACCGAATACGAATACCCAAAAGGGCAATGGCGAATACTATATCCAAGGAATTGGTTCAGCCCTCCTCCTAGTAAAAACACAACCAGGGTTTGTTTCGATTGCGATACGAACACTACTCCTCTTTGGAGGAGTGATCCTACGGGTTCCAAGTCGTTGTGCAATGCATGTGGACTCCGACGAAGAAGGGAAGAGGCAAAAGCCAATAAGTCTCTTGACACTGAAAAGGGAAAGGGAAACGGCGAAAGCTCATCATCTGAGAATGTGAAGAAGTTTTAA

Protein sequence

>Msa0053300

MRVSNDDPVPATKKKKTAASAAKKKKSVASASKKKIKTATEYEYPKGQWRILYPRNWFSPPPSKNTTRVCFDCDTNTTPLWRSDPTGSKSLCNACGLRRRREEAKANKSLDTEKGKGNGESSSSENVKKF*