AlfalfaGEDB | Msa0056950

M. sativa cultivar zhongmu-4 / Msa0056950

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0056950	sp\|Q9S7G7\|MB3R1_ARATH	65.306	147	51	0	5	151	25	171	6.38e-66	216

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0056950	A0A896WCC0	97.973	148	3	0	4	151	32	179	1.89e-104	309

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TFs/TRs:

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Gene ID	Type	Classification
Msa0056950	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0056950	MtrunA17_Chr1g0157731	66.667	147	49	0	5	151	29	175	8.69e-68	221
Msa0056950	MtrunA17_Chr3g0139671	66.667	147	49	0	5	151	23	169	1.14e-67	220
Msa0056950	MtrunA17_Chr7g0238631	63.333	150	54	1	2	151	49	197	6.23e-64	204
Msa0056950	MtrunA17_Chr5g0397841	57.042	142	58	1	13	151	53	194	4.55e-51	169
Msa0056950	MtrunA17_Chr3g0117511	59.091	88	36	0	64	151	180	267	1.09e-29	112
Msa0056950	MtrunA17_Chr3g0117511	41.053	95	55	1	15	109	183	276	4.49e-16	73.9
Msa0056950	MtrunA17_Chr5g0429661	58.621	87	36	0	65	151	120	206	1.58e-29	110
Msa0056950	MtrunA17_Chr5g0429661	39.583	96	55	2	15	109	122	215	1.96e-15	72.0
Msa0056950	MtrunA17_Chr4g0058991	58.621	87	36	0	65	151	207	293	9.19e-29	109
Msa0056950	MtrunA17_Chr4g0058991	34.746	118	67	2	1	109	186	302	1.06e-14	70.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0056950	AT4G32730.5	65.306	147	51	0	5	151	25	171	5.05e-67	219
Msa0056950	AT4G32730.4	65.306	147	51	0	5	151	25	171	5.05e-67	219
Msa0056950	AT4G32730.3	65.306	147	51	0	5	151	25	171	5.05e-67	219
Msa0056950	AT4G32730.2	65.306	147	51	0	5	151	25	171	5.05e-67	219
Msa0056950	AT4G32730.1	65.306	147	51	0	5	151	25	171	6.49e-67	216
Msa0056950	AT5G02320.3	65.541	148	50	1	4	151	5	151	6.54e-66	208
Msa0056950	AT5G02320.1	65.541	148	50	1	4	151	65	211	1.52e-65	209
Msa0056950	AT5G02320.2	65.541	148	50	1	4	151	65	211	1.52e-65	209
Msa0056950	AT5G11510.5	61.905	147	56	0	5	151	19	165	1.43e-63	207
Msa0056950	AT5G11510.2	61.905	147	56	0	5	151	19	165	1.91e-63	207

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Find 36 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCCATGGACCAAAGAAG+AGG	0.612896	1_2:-12725583	Msa0056950:CDS
AAGTCCTTACCAGGTCGCAT+AGG	0.617150	1_2:-12725768	Msa0056950:CDS
ACAAAATATATGGGAATAGT+TGG	0.337373	1_2:-12725539	Msa0056950:CDS
ACAAGAAGATCAACTAAAGG+AGG	0.608718	1_2:-12726709	Msa0056950:CDS
AGCGAGCTTGTTAAGATAAA+TGG	0.141546	1_2:-12725816	Msa0056950:CDS
AGGAGGCTGGACAGAGAAGG+AGG	0.589108	1_2:-12726692	Msa0056950:intron
ATACCTTCCATGCAGAAATT+TGG	0.243914	1_2:+12725506	None:intergenic
ATCAAGACTCACAAAATATA+TGG	0.210499	1_2:-12725549	Msa0056950:CDS
ATGACTAATAGAAGGGTGAC+TGG	0.565778	1_2:-12726736	Msa0056950:CDS
ATGTCTGCATCGCTGGCAAA+AGG	0.482193	1_2:-12726005	Msa0056950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATTGGCAAGAAATTCTCTT+TGG	+	chr1_2:12725631-12725650	None:intergenic	30.0%
GGAAAAAGTTGGAAAAAGAT+AGG	-	chr1_2:12725743-12725762	Msa0056950:intron	30.0%
GCTATTGAAATAGTCAGATT+TGG	-	chr1_2:12725837-12725856	Msa0056950:intron	30.0%
TAGGTAAACAATGTAGAGAA+AGG	-	chr1_2:12726490-12726509	Msa0056950:intron	30.0%
CAGATATAAAGAAAACTCCA+TGG	-	chr1_2:12726643-12726662	Msa0056950:intron	30.0%
TATTGGCAAGAAATTCTCTT+TGG	+	chr1_2:12725631-12725650	None:intergenic	30.0%
GGAAAAAGTTGGAAAAAGAT+AGG	-	chr1_2:12725743-12725762	Msa0056950:intron	30.0%
GCTATTGAAATAGTCAGATT+TGG	-	chr1_2:12725837-12725856	Msa0056950:intron	30.0%
TAGGTAAACAATGTAGAGAA+AGG	-	chr1_2:12726490-12726509	Msa0056950:intron	30.0%
CAGATATAAAGAAAACTCCA+TGG	-	chr1_2:12726643-12726662	Msa0056950:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	12725503	12726758	12725503	ID=Msa0056950;Name=Msa0056950
chr1_2	mRNA	12725503	12726758	12725503	ID=Msa0056950-mRNA-1;Parent=Msa0056950;Name=Msa0056950-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|0\|0\|0.8\|1\|1\|5\|0\|152
chr1_2	exon	12726693	12726758	12726693	ID=Msa0056950-mRNA-1:exon:4978;Parent=Msa0056950-mRNA-1
chr1_2	exon	12726497	12726554	12726497	ID=Msa0056950-mRNA-1:exon:4977;Parent=Msa0056950-mRNA-1
chr1_2	exon	12725964	12726058	12725964	ID=Msa0056950-mRNA-1:exon:4976;Parent=Msa0056950-mRNA-1
chr1_2	exon	12725750	12725853	12725750	ID=Msa0056950-mRNA-1:exon:4975;Parent=Msa0056950-mRNA-1
chr1_2	exon	12725503	12725638	12725503	ID=Msa0056950-mRNA-1:exon:4974;Parent=Msa0056950-mRNA-1
chr1_2	CDS	12726693	12726758	12726693	ID=Msa0056950-mRNA-1:cds;Parent=Msa0056950-mRNA-1
chr1_2	CDS	12726497	12726554	12726497	ID=Msa0056950-mRNA-1:cds;Parent=Msa0056950-mRNA-1
chr1_2	CDS	12725964	12726058	12725964	ID=Msa0056950-mRNA-1:cds;Parent=Msa0056950-mRNA-1
chr1_2	CDS	12725750	12725853	12725750	ID=Msa0056950-mRNA-1:cds;Parent=Msa0056950-mRNA-1
chr1_2	CDS	12725503	12725638	12725503	ID=Msa0056950-mRNA-1:cds;Parent=Msa0056950-mRNA-1

Gene Sequence

>Msa0056950

ATGACTAATAGAAGGGTGACTGGTCCTACAAGAAGATCAACTAAAGGAGGCTGGACAGAGAAGGAGGATAAACTTTTGATTGCTGCTGTGAAAAAGTATAATGGAAAAAGTTGGAAAAAGATAGCTGCTTGTGTTACTGGCAGAACAGATGTTCAATGTCTGCATCGCTGGCAAAAGGTTCTGAATCCTGATCTTATCAAAGGACCTTGGTCAGAACAGGAAGATAATCAACTAAGCGAGCTTGTTAAGATAAATGGGAAAAAAAATTGGTCTCAGGTAGCAAAGTCCTTACCAGGTCGCATAGGTAAACAATGTAGAGAAAGGTGGTATAACCATTTAAATCCAGATATAAAGAAAACTCCATGGACCAAAGAAGAGGAGTCGACTCTAATCAAGACTCACAAAATATATGGGAATAGTTGGGCTGAAATAGCCAAATTTCTGCATGGAAGGTATTGA

Protein sequence

>Msa0056950

MTNRRVTGPTRRSTKGGWTEKEDKLLIAAVKKYNGKSWKKIAACVTGRTDVQCLHRWQKVLNPDLIKGPWSEQEDNQLSELVKINGKKNWSQVAKSLPGRIGKQCRERWYNHLNPDIKKTPWTKEEESTLIKTHKIYGNSWAEIAKFLHGRY*