Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0057030 | QSD99710.1 | 97.973 | 148 | 3 | 0 | 4 | 151 | 32 | 179 | 3.96e-104 | 309 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0057030 | sp|Q9S7G7|MB3R1_ARATH | 65.306 | 147 | 51 | 0 | 5 | 151 | 25 | 171 | 6.38e-66 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0057030 | A0A896WCC0 | 97.973 | 148 | 3 | 0 | 4 | 151 | 32 | 179 | 1.89e-104 | 309 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0057030 | MtrunA17_Chr1g0157731 | 66.667 | 147 | 49 | 0 | 5 | 151 | 29 | 175 | 8.69e-68 | 221 |
| Msa0057030 | MtrunA17_Chr3g0139671 | 66.667 | 147 | 49 | 0 | 5 | 151 | 23 | 169 | 1.14e-67 | 220 |
| Msa0057030 | MtrunA17_Chr7g0238631 | 63.333 | 150 | 54 | 1 | 2 | 151 | 49 | 197 | 6.23e-64 | 204 |
| Msa0057030 | MtrunA17_Chr5g0397841 | 57.042 | 142 | 58 | 1 | 13 | 151 | 53 | 194 | 4.55e-51 | 169 |
| Msa0057030 | MtrunA17_Chr3g0117511 | 59.091 | 88 | 36 | 0 | 64 | 151 | 180 | 267 | 1.09e-29 | 112 |
| Msa0057030 | MtrunA17_Chr3g0117511 | 41.053 | 95 | 55 | 1 | 15 | 109 | 183 | 276 | 4.49e-16 | 73.9 |
| Msa0057030 | MtrunA17_Chr5g0429661 | 58.621 | 87 | 36 | 0 | 65 | 151 | 120 | 206 | 1.58e-29 | 110 |
| Msa0057030 | MtrunA17_Chr5g0429661 | 39.583 | 96 | 55 | 2 | 15 | 109 | 122 | 215 | 1.96e-15 | 72.0 |
| Msa0057030 | MtrunA17_Chr4g0058991 | 58.621 | 87 | 36 | 0 | 65 | 151 | 207 | 293 | 9.19e-29 | 109 |
| Msa0057030 | MtrunA17_Chr4g0058991 | 34.746 | 118 | 67 | 2 | 1 | 109 | 186 | 302 | 1.06e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0057030 | AT4G32730.5 | 65.306 | 147 | 51 | 0 | 5 | 151 | 25 | 171 | 5.05e-67 | 219 |
| Msa0057030 | AT4G32730.4 | 65.306 | 147 | 51 | 0 | 5 | 151 | 25 | 171 | 5.05e-67 | 219 |
| Msa0057030 | AT4G32730.3 | 65.306 | 147 | 51 | 0 | 5 | 151 | 25 | 171 | 5.05e-67 | 219 |
| Msa0057030 | AT4G32730.2 | 65.306 | 147 | 51 | 0 | 5 | 151 | 25 | 171 | 5.05e-67 | 219 |
| Msa0057030 | AT4G32730.1 | 65.306 | 147 | 51 | 0 | 5 | 151 | 25 | 171 | 6.49e-67 | 216 |
| Msa0057030 | AT5G02320.3 | 65.541 | 148 | 50 | 1 | 4 | 151 | 5 | 151 | 6.54e-66 | 208 |
| Msa0057030 | AT5G02320.1 | 65.541 | 148 | 50 | 1 | 4 | 151 | 65 | 211 | 1.52e-65 | 209 |
| Msa0057030 | AT5G02320.2 | 65.541 | 148 | 50 | 1 | 4 | 151 | 65 | 211 | 1.52e-65 | 209 |
| Msa0057030 | AT5G11510.5 | 61.905 | 147 | 56 | 0 | 5 | 151 | 19 | 165 | 1.43e-63 | 207 |
| Msa0057030 | AT5G11510.2 | 61.905 | 147 | 56 | 0 | 5 | 151 | 19 | 165 | 1.91e-63 | 207 |
Find 36 sgRNAs with CRISPR-Local
Find 148 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCCATGGACCAAAGAAG+AGG | 0.612896 | 1_2:-12910987 | Msa0057030:CDS |
| AAGTCCTTACCAGGTCGCAT+AGG | 0.617150 | 1_2:-12911172 | Msa0057030:CDS |
| ACAAAATATATGGGAATAGT+TGG | 0.337373 | 1_2:-12910943 | Msa0057030:CDS |
| ACAAGAAGATCAACTAAAGG+AGG | 0.608718 | 1_2:-12912113 | Msa0057030:CDS |
| AGCGAGCTTGTTAAGATAAA+TGG | 0.141546 | 1_2:-12911220 | Msa0057030:CDS |
| AGGAGGCTGGACAGAGAAGG+AGG | 0.589108 | 1_2:-12912096 | Msa0057030:intron |
| ATACCTTCCATGCAGAAATT+TGG | 0.243914 | 1_2:+12910910 | None:intergenic |
| ATCAAGACTCACAAAATATA+TGG | 0.210499 | 1_2:-12910953 | Msa0057030:CDS |
| ATGACTAATAGAAGGGTGAC+TGG | 0.565778 | 1_2:-12912140 | Msa0057030:CDS |
| ATGTCTGCATCGCTGGCAAA+AGG | 0.482193 | 1_2:-12911409 | Msa0057030:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTGGCAAGAAATTCTCTT+TGG | + | chr1_2:12911035-12911054 | None:intergenic | 30.0% | |
| GGAAAAAGTTGGAAAAAGAT+AGG | - | chr1_2:12911147-12911166 | Msa0057030:intron | 30.0% | |
| GCTATTGAAATAGTCAGATT+TGG | - | chr1_2:12911241-12911260 | Msa0057030:intron | 30.0% | |
| TAGGTAAACAATGTAGAGAA+AGG | - | chr1_2:12911894-12911913 | Msa0057030:intron | 30.0% | |
| CAGATATAAAGAAAACTCCA+TGG | - | chr1_2:12912047-12912066 | Msa0057030:intron | 30.0% | |
| TATTGGCAAGAAATTCTCTT+TGG | + | chr1_2:12911035-12911054 | None:intergenic | 30.0% | |
| GGAAAAAGTTGGAAAAAGAT+AGG | - | chr1_2:12911147-12911166 | Msa0057030:intron | 30.0% | |
| GCTATTGAAATAGTCAGATT+TGG | - | chr1_2:12911241-12911260 | Msa0057030:intron | 30.0% | |
| TAGGTAAACAATGTAGAGAA+AGG | - | chr1_2:12911894-12911913 | Msa0057030:intron | 30.0% | |
| CAGATATAAAGAAAACTCCA+TGG | - | chr1_2:12912047-12912066 | Msa0057030:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 12910907 | 12912162 | 12910907 | ID=Msa0057030;Name=Msa0057030 |
| chr1_2 | mRNA | 12910907 | 12912162 | 12910907 | ID=Msa0057030-mRNA-1;Parent=Msa0057030;Name=Msa0057030-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0|0|0.8|1|1|5|0|152 |
| chr1_2 | exon | 12912097 | 12912162 | 12912097 | ID=Msa0057030-mRNA-1:exon:5063;Parent=Msa0057030-mRNA-1 |
| chr1_2 | exon | 12911901 | 12911958 | 12911901 | ID=Msa0057030-mRNA-1:exon:5062;Parent=Msa0057030-mRNA-1 |
| chr1_2 | exon | 12911368 | 12911462 | 12911368 | ID=Msa0057030-mRNA-1:exon:5061;Parent=Msa0057030-mRNA-1 |
| chr1_2 | exon | 12911154 | 12911257 | 12911154 | ID=Msa0057030-mRNA-1:exon:5060;Parent=Msa0057030-mRNA-1 |
| chr1_2 | exon | 12910907 | 12911042 | 12910907 | ID=Msa0057030-mRNA-1:exon:5059;Parent=Msa0057030-mRNA-1 |
| chr1_2 | CDS | 12912097 | 12912162 | 12912097 | ID=Msa0057030-mRNA-1:cds;Parent=Msa0057030-mRNA-1 |
| chr1_2 | CDS | 12911901 | 12911958 | 12911901 | ID=Msa0057030-mRNA-1:cds;Parent=Msa0057030-mRNA-1 |
| chr1_2 | CDS | 12911368 | 12911462 | 12911368 | ID=Msa0057030-mRNA-1:cds;Parent=Msa0057030-mRNA-1 |
| chr1_2 | CDS | 12911154 | 12911257 | 12911154 | ID=Msa0057030-mRNA-1:cds;Parent=Msa0057030-mRNA-1 |
| chr1_2 | CDS | 12910907 | 12911042 | 12910907 | ID=Msa0057030-mRNA-1:cds;Parent=Msa0057030-mRNA-1 |
| Gene Sequence |
>Msa0057030 ATGACTAATAGAAGGGTGACTGGTCCTACAAGAAGATCAACTAAAGGAGGCTGGACAGAGAAGGAGGATAAACTTTTGATTGCTGCTGTGAAAAAGTATAATGGAAAAAGTTGGAAAAAGATAGCTGCTTGTGTTACTGGCAGAACAGATGTTCAATGTCTGCATCGCTGGCAAAAGGTTCTGAATCCTGATCTTATCAAAGGACCTTGGTCAGAACAGGAAGATAATCAACTAAGCGAGCTTGTTAAGATAAATGGGAAAAAAAATTGGTCTCAGGTAGCAAAGTCCTTACCAGGTCGCATAGGTAAACAATGTAGAGAAAGGTGGTATAACCATTTAAATCCAGATATAAAGAAAACTCCATGGACCAAAGAAGAGGAGTCGACTCTAATCAAGACTCACAAAATATATGGGAATAGTTGGGCTGAAATAGCCAAATTTCTGCATGGAAGGTATTGA |
| Protein sequence |
>Msa0057030 MTNRRVTGPTRRSTKGGWTEKEDKLLIAAVKKYNGKSWKKIAACVTGRTDVQCLHRWQKVLNPDLIKGPWSEQEDNQLSELVKINGKKNWSQVAKSLPGRIGKQCRERWYNHLNPDIKKTPWTKEEESTLIKTHKIYGNSWAEIAKFLHGRY* |
