AlfalfaGEDB | Msa0059500

M. sativa cultivar zhongmu-4 / Msa0059500

KEGG: -
GO: -
PFAM: PF13947.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0059500	sp\|P93604\|LRK10_WHEAT	39.905	629	305	19	29	619	34	627	2.00e-132	406

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0059500	A0A396JMB6	96.794	655	21	0	1	655	1	655	0.0	1318

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0059500	MtrunA17_Chr1g0158761	96.794	655	21	0	1	655	1	655	1318
Msa0059500	MtrunA17_Chr1g0157791	50.423	710	277	14	11	655	6	705	658
Msa0059500	MtrunA17_Chr1g0157991	52.259	664	252	13	24	627	28	686	651
Msa0059500	MtrunA17_Chr1g0158411	50.901	666	255	12	22	625	40	695	635
Msa0059500	MtrunA17_Chr1g0158151	49.779	679	275	15	3	622	2	673	629
Msa0059500	MtrunA17_Chr6g0467711	51.534	652	258	12	29	625	43	691	625
Msa0059500	MtrunA17_Chr1g0158191	48.692	688	274	14	3	622	2	678	621
Msa0059500	MtrunA17_Chr1g0158631	49.475	667	272	14	10	622	17	672	614
Msa0059500	MtrunA17_Chr1g0158301	49.323	665	284	14	4	618	17	678	613
Msa0059500	MtrunA17_Chr1g0158661	49.541	654	277	15	10	622	17	658	613

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0059500	AT1G66920.2	35.703	619	336	18	17	618	19	592	9.86e-109	342
Msa0059500	AT5G38260.1	35.368	639	336	23	10	618	16	607	1.20e-108	342
Msa0059500	AT1G67000.1	35.360	625	353	21	24	618	249	852	4.46e-108	347
Msa0059500	AT5G38250.1	35.804	634	330	21	17	628	23	601	1.83e-107	338
Msa0059500	AT1G66910.2	35.593	649	358	22	1	618	12	631	2.80e-107	339
Msa0059500	AT1G66910.1	35.394	647	365	21	1	618	12	634	9.12e-107	338
Msa0059500	AT1G66920.1	35.057	619	332	18	17	618	19	584	9.52e-107	336
Msa0059500	AT5G38240.3	48.476	361	160	9	277	628	215	558	3.36e-106	333
Msa0059500	AT5G38240.2	48.476	361	160	9	277	628	261	604	1.11e-105	333
Msa0059500	AT5G38240.1	48.476	361	160	9	277	628	279	622	1.83e-105	333

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Find 129 sgRNAs with CRISPR-Local

Find 322 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTTTGATATAGCTCT+TGG	0.488922	1_2:+16340754	Msa0059500:CDS
AAACTTCACTCCAAAGGTTT+CGG	0.385246	1_2:+16340867	Msa0059500:CDS
AAACTTGGCGAAGGTGGCTA+TGG	0.514946	1_2:+16340475	Msa0059500:CDS
AAAGCAGATGTTTATAGCTT+TGG	0.293855	1_2:+16341012	Msa0059500:CDS
AAAGGATTTAAGGATAAACT+TGG	0.544758	1_2:+16340460	Msa0059500:CDS
AAAGTGGTTGAGTTGCTTGA+AGG	0.503408	1_2:+16341258	Msa0059500:CDS
AACATGGTGAATCGTTCCAA+TGG	0.614240	1_2:-16340602	None:intergenic
AACCTACCTTGGCTGCTACC+CGG	0.555240	1_2:+16341363	Msa0059500:CDS
AACGATTCACCATGTTAATG+TGG	0.592628	1_2:+16340609	Msa0059500:CDS
AAGAGGAACTATGGGATACA+TGG	0.574428	1_2:+16340948	Msa0059500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCACACGAAAGTTCATAATT+TGG	-	chr1_2:16339175-16339194	None:intergenic	30.0%
TGAATCTATGATCCTTATTG+TGG	-	chr1_2:16339266-16339285	None:intergenic	30.0%
ACAATAAGGATCATAGATTC+AGG	+	chr1_2:16339265-16339284	Msa0059500:CDS	30.0%
GATACTTCATCTTGTAACAA+AGG	+	chr1_2:16339442-16339461	Msa0059500:CDS	30.0%
TCTTCCAATTATGCTGTATT+CGG	+	chr1_2:16339475-16339494	Msa0059500:CDS	30.0%
TAAGCTGAACAACAAAAATG+AGG	+	chr1_2:16339681-16339700	Msa0059500:CDS	30.0%
AACAACAAAAATGAGGTCAT+TGG	+	chr1_2:16339688-16339707	Msa0059500:CDS	30.0%
TTTACAGGTTCTCACATATT+AGG	+	chr1_2:16340136-16340155	Msa0059500:intron	30.0%
ATTTATATGCCATGTACATC+GGG	-	chr1_2:16340333-16340352	None:intergenic	30.0%
CATTTATATGCCATGTACAT+CGG	-	chr1_2:16340334-16340353	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	16339007	16341428	16339007	ID=Msa0059500;Name=Msa0059500
chr1_2	mRNA	16339007	16341428	16339007	ID=Msa0059500-mRNA-1;Parent=Msa0059500;Name=Msa0059500-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0\|0\|0\|1\|1\|1\|4\|0\|655
chr1_2	exon	16339007	16339760	16339007	ID=Msa0059500-mRNA-1:exon:6568;Parent=Msa0059500-mRNA-1
chr1_2	exon	16339869	16339913	16339869	ID=Msa0059500-mRNA-1:exon:6569;Parent=Msa0059500-mRNA-1
chr1_2	exon	16340143	16340169	16340143	ID=Msa0059500-mRNA-1:exon:6570;Parent=Msa0059500-mRNA-1
chr1_2	exon	16340287	16341428	16340287	ID=Msa0059500-mRNA-1:exon:6571;Parent=Msa0059500-mRNA-1
chr1_2	CDS	16339007	16339760	16339007	ID=Msa0059500-mRNA-1:cds;Parent=Msa0059500-mRNA-1
chr1_2	CDS	16339869	16339913	16339869	ID=Msa0059500-mRNA-1:cds;Parent=Msa0059500-mRNA-1
chr1_2	CDS	16340143	16340169	16340143	ID=Msa0059500-mRNA-1:cds;Parent=Msa0059500-mRNA-1
chr1_2	CDS	16340287	16341428	16340287	ID=Msa0059500-mRNA-1:cds;Parent=Msa0059500-mRNA-1

Gene Sequence

>Msa0059500

ATGTCTTGGAAAATTCTTTTTAATCTAATTGTTTTAATGATCCTCCTATACCCTCTTCCTTGCCTAACAGAAACAAATCACCAATGTATCCCTTCATCTTGTGGCAAAATACAAAACATCACTTACCCTTTTCGTCTACAAACCGATCCCGAAAACTGTGGTGATCCAAATTATGAACTTTCGTGTGAGAACAATCAAACAATACTAAATCTATACAATGGAAAATACTTTGTAGAATCCATCAACTACAAAAATTCCACAATAAGGATCATAGATTCAGGTCTCAACAAAGAAAATTGCTCTTCAATTCCTCTCTACACTTTAACAAATGAAAACTTCACCACTGAAAAACCTTATAGCTTGTTAGATACTAATAACTTAGAAACCATAGCATTTATACGCTGTGCAGCTTCAATTAATTCTCCACTTTACATGGATACTTCATCTTGTAACAAAGGTTTAATCAACTCTTCCAATTATGCTGTATTCGGTGAGTTGAAAATCTCTGAGTTGGAAGATTCATGCAGCATACAAGTCATGGTTGTTATGACTTCAAATTTGAACAACGGCGGCAAAAACTTTTCATATGTGAATATTCATCAGCAACTTGTTTATGGATTGGAGCTTTCTTGGCATGAGATATTCTGCAAAGAGTGTCAAGGTAGAGGGTACTGTAAGCTGAACAACAAAAATGAGGTCATTGGTTGTTCAAGAAGTAGACCTTGTAGTCACGACTATATACCGTCTTTTAAATGTGCTTTAAAAGAGCTAATCCATACAGCCAAAGATGCGACTAAAGGTTCTCACATATTAGGCGCTCTCATCGGAATAATTCTTCTGATAAGAATCATAGGTGGTTTACTATTTATCCCGATGTACATGGCATATAAATGGAGAAGGAGGCATTTATCTGCATATGATTCAGTTGAAGAGTTTCTTAAATCTCACAATAATCTCATGCCAATAAGATACTCGTACTCGGACATTAAAAAAATGACAAAAGGATTTAAGGATAAACTTGGCGAAGGTGGCTATGGTTCTGTTTACAAAGGACAACTTCGTAATGGTCACCTAGTAGCAATCAAGTTGCTAGGTGGAAAATCAAGAGCAAATGGACAAGAATTCATCAATGAAGTTGCTACCATTGGAACGATTCACCATGTTAATGTGGTTCACCTAATTGGCTTTTGTGTTGAGAAAACAAAAAGAGCACTTGTTTATGAGTTCATGCCTAATGGTTCTCTTGAAAAACACATTTTCTCAAGAGAAAGAATGGGTTCTTTGAGTTCCGAGAAAATGTTTGATATAGCTCTTGGTGTAGCGCGTGGTATTGAATATCTACATAGAGGGTGTGACATGAGGATTTTGCATTTTGACATAAAGCCTCATAACATTCTTCTTGACGTAAACTTCACTCCAAAGGTTTCGGATTTTGGACTAGCAAAGTTATATCCAACGGACAATAGCATTGTGTCTCTTACAGCTGCAAGAGGAACTATGGGATACATGGCGCCAGAATTGTTCTATAAGAATATTGGAGGAGTATCGTACAAAGCAGATGTTTATAGCTTTGGAATGTTGTTAATGGAAATGGCGAGTAGAAGAAGGAACATGAATGAGGGTGAAGATCATAAGAGTCAAGTTTATTTCCCTTCTTGGGTTTACGACCAATTTAGCGAAGGGAAGGAAATAGATATGGGGAATGGTACAGAAGAGGAGGAGAAGATACTGAAGAAGATGATAATAGTTGCATTGTGGTGTATACAGATGAAACCAATTGATCGTCCTTCGATGAACAAAGTGGTTGAGTTGCTTGAAGGAGATGTTGAGTCCTTGGAAATGCCTCCTAAGCCATTTCAAACTCCTAAAGGAATGCCTGTACAAGGCATTGGACAACTCAGAAACCTACCTTGGCTGCTACCCGGAGATTCTACAAACTCACTTACCATTGTTATAAATCGACGTTAA

Protein sequence

>Msa0059500

MSWKILFNLIVLMILLYPLPCLTETNHQCIPSSCGKIQNITYPFRLQTDPENCGDPNYELSCENNQTILNLYNGKYFVESINYKNSTIRIIDSGLNKENCSSIPLYTLTNENFTTEKPYSLLDTNNLETIAFIRCAASINSPLYMDTSSCNKGLINSSNYAVFGELKISELEDSCSIQVMVVMTSNLNNGGKNFSYVNIHQQLVYGLELSWHEIFCKECQGRGYCKLNNKNEVIGCSRSRPCSHDYIPSFKCALKELIHTAKDATKGSHILGALIGIILLIRIIGGLLFIPMYMAYKWRRRHLSAYDSVEEFLKSHNNLMPIRYSYSDIKKMTKGFKDKLGEGGYGSVYKGQLRNGHLVAIKLLGGKSRANGQEFINEVATIGTIHHVNVVHLIGFCVEKTKRALVYEFMPNGSLEKHIFSRERMGSLSSEKMFDIALGVARGIEYLHRGCDMRILHFDIKPHNILLDVNFTPKVSDFGLAKLYPTDNSIVSLTAARGTMGYMAPELFYKNIGGVSYKADVYSFGMLLMEMASRRRNMNEGEDHKSQVYFPSWVYDQFSEGKEIDMGNGTEEEEKILKKMIIVALWCIQMKPIDRPSMNKVVELLEGDVESLEMPPKPFQTPKGMPVQGIGQLRNLPWLLPGDSTNSLTIVINRR*