AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0060940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0060940 MtrunA17_Chr5g0437131 84.667 300 38 1 367 658 239 538 6.06e-160 486
Msa0060940 MtrunA17_Chr8g0371291 79.195 149 24 1 659 807 81 222 2.97e-81 262
Msa0060940 MtrunA17_Chr6g0480541 61.991 221 62 5 492 700 859 1069 4.57e-72 259
Msa0060940 MtrunA17_Chr5g0406031 68.000 150 47 1 658 807 83 231 2.88e-63 213
Msa0060940 MtrunA17_Chr6g0480481 52.174 161 33 2 506 658 9 133 1.53e-41 151
Msa0060940 MtrunA17_Chr3g0126701 84.337 83 13 0 807 889 510 592 3.81e-39 157
Msa0060940 MtrunA17_Chr2g0286611 69.811 106 31 1 113 217 10 115 1.86e-35 140
Msa0060940 MtrunA17_Chr1g0147121 61.728 81 31 0 811 891 483 563 4.05e-26 115
Msa0060940 MtrunA17_Chr8g0369241 38.503 187 92 6 489 658 91 271 1.27e-24 110
Msa0060940 MtrunA17_Chr4g0060981 35.443 158 86 4 658 807 67 216 5.93e-21 92.4
Msa0060940 MtrunA17_Chr3g0081691 70.833 72 21 0 181 252 195 266 1.40e-18 91.7
Msa0060940 MtrunA17_Chr8g0389491 83.333 66 8 1 6 71 1 63 1.89e-16 79.3
Msa0060940 MtrunA17_Chr8g0389491 77.083 48 11 0 69 116 143 190 1.30e-14 73.9
Msa0060940 MtrunA17_Chr7g0275611 34.746 118 75 2 808 924 425 541 2.44e-15 80.9
Msa0060940 MtrunA17_Chr8g0347071 42.105 76 44 0 808 883 410 485 3.43e-15 80.5
Msa0060940 MtrunA17_Chr5g0444161 33.333 132 66 6 687 807 107 227 9.47e-14 72.0
Msa0060940 MtrunA17_Chr1g0210061 33.562 146 80 4 526 658 605 746 1.05e-12 72.4
Msa0060940 MtrunA17_Chr5g0444141 34.400 125 74 3 687 807 142 262 3.22e-12 68.2
Msa0060940 MtrunA17_Chr6g0451031 48.889 45 23 0 763 807 67 111 3.56e-12 64.7
Msa0060940 MtrunA17_Chr4g0067591 35.065 77 45 1 731 807 75 146 5.69e-12 67.4
Msa0060940 MtrunA17_Chr7g0246761 32.558 129 64 5 685 807 21 132 6.14e-12 65.5
Msa0060940 MtrunA17_Chr3g0128771 41.026 78 39 2 735 809 90 163 7.38e-12 65.1
Msa0060940 MtrunA17_Chr8g0388231 43.421 76 42 1 736 811 309 383 8.41e-12 68.6
Msa0060940 MtrunA17_Chr8g0366561 43.243 74 41 1 736 809 114 186 1.49e-11 67.4
Msa0060940 MtrunA17_Chr5g0411081 37.209 86 47 1 722 807 112 190 1.73e-11 67.0
Msa0060940 MtrunA17_Chr8g0369571 48.889 45 23 0 763 807 88 132 3.29e-11 63.2
Msa0060940 MtrunA17_Chr5g0437361 38.750 80 48 1 735 814 74 152 6.71e-11 62.0
Msa0060940 MtrunA17_Chr7g0273051 36.986 73 46 0 735 807 90 162 9.69e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0060940 AT2G40120.1 72.941 170 38 1 497 658 227 396 3.25e-75 258
Msa0060940 AT1G73460.2 59.276 221 68 5 492 700 819 1029 5.87e-69 251
Msa0060940 AT1G73460.1 59.276 221 68 5 492 700 819 1029 5.87e-69 251
Msa0060940 AT1G73450.1 59.276 221 68 5 492 700 802 1012 7.35e-69 250
Msa0060940 AT3G17750.1 64.571 175 54 1 492 658 788 962 4.52e-65 239
Msa0060940 AT2G17730.1 64.667 150 53 0 658 807 89 238 3.26e-61 208
Msa0060940 AT4G35840.1 64.667 150 53 0 658 807 84 233 1.03e-60 207
Msa0060940 AT5G66070.5 63.758 149 49 1 659 807 75 218 4.15e-60 204
Msa0060940 AT5G66070.1 63.758 149 49 1 659 807 75 218 4.15e-60 204
Msa0060940 AT2G17730.2 64.336 143 51 0 658 800 89 231 1.81e-56 196
Msa0060940 AT4G35840.2 53.297 182 53 1 658 807 84 265 3.39e-54 190
Msa0060940 AT5G66070.2 54.598 174 50 2 658 807 74 242 7.29e-52 182
Msa0060940 AT5G66070.4 58.929 112 41 1 659 770 75 181 2.22e-35 133
Msa0060940 AT5G66070.3 48.529 136 41 2 659 770 75 205 2.13e-30 120
Msa0060940 AT1G74410.1 36.000 150 93 1 660 809 74 220 2.85e-25 105
Msa0060940 AT5G53430.1 50.000 108 33 2 803 889 463 570 4.49e-25 113
Msa0060940 AT5G35980.1 37.705 183 91 6 507 672 92 268 3.35e-23 107
Msa0060940 AT5G35980.2 37.705 183 91 6 507 672 92 268 3.87e-23 106
Msa0060940 AT5G35980.3 37.705 183 91 6 507 672 57 233 4.18e-23 106
Msa0060940 AT4G27910.1 48.148 108 35 2 803 889 448 555 8.31e-23 105
Msa0060940 AT3G20395.1 33.333 123 76 2 687 808 97 214 1.61e-15 77.4
Msa0060940 AT3G61740.2 41.667 72 42 0 814 885 444 515 2.32e-14 78.2
Msa0060940 AT3G61740.1 41.667 72 42 0 814 885 444 515 2.34e-14 78.2
Msa0060940 AT3G18773.1 39.560 91 49 2 730 816 97 185 5.60e-13 69.7
Msa0060940 AT1G49200.1 41.892 74 41 1 734 807 107 178 1.76e-12 68.6
Msa0060940 AT2G17450.1 37.179 78 45 1 734 807 67 144 8.93e-12 65.5
Msa0060940 AT5G05280.1 41.333 75 40 1 735 809 87 157 3.08e-11 63.5
Msa0060940 AT2G27940.1 39.241 79 38 2 735 810 114 185 3.38e-11 65.1
Msa0060940 AT3G16720.1 38.356 73 41 1 735 807 93 161 3.64e-11 65.9
Msa0060940 AT5G17600.1 40.000 90 49 2 718 807 100 184 3.72e-11 66.6
Msa0060940 AT1G49210.1 40.541 74 42 1 734 807 106 177 4.65e-11 64.3
Msa0060940 AT5G01880.1 41.558 77 41 2 734 809 76 149 5.71e-11 62.4
Msa0060940 AT1G72310.1 37.838 74 42 1 736 809 102 171 8.38e-11 65.1

Find 204 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAGCTACCTTAATTTAA+AGG 0.143063 1_2:-18920872 Msa0060940:CDS
GGATCCTTGATTCATCTTTC+TGG 0.165412 1_2:-18921249 Msa0060940:CDS
GAGAACCAATTCCGGTTATT+AGG 0.172098 1_2:-18922264 Msa0060940:CDS
TTAACATAATCAATTGTCTT+AGG 0.186245 1_2:+18921603 None:intergenic
CTTTGGCATTCGAATAATTC+TGG 0.197162 1_2:-18920049 Msa0060940:CDS
CAAGCTCCACCTAGACCTTT+AGG 0.219512 1_2:-18921777 Msa0060940:CDS
CATAAATTCAACCAAGAATA+TGG 0.227119 1_2:-18920226 Msa0060940:CDS
GCTTAATTTCCTTTAAATTA+AGG 0.237843 1_2:+18920863 None:intergenic
AAGCTCCACCTAGACCTTTA+GGG 0.263196 1_2:-18921776 Msa0060940:CDS
ATGCTTCACCACCATATTCT+TGG 0.272074 1_2:+18920215 None:intergenic
TTAAGGTAGCTAATGTTTAT+AGG 0.288253 1_2:+18920880 None:intergenic
TGCTCACTGTCTCTGCATAA+TGG 0.302576 1_2:+18919653 None:intergenic
ACAGAAAAGCAAGCACTTAA+TGG 0.303631 1_2:-18919530 Msa0060940:CDS
TTCCTGTTCAATTTGCCTTC+AGG 0.305106 1_2:-18919774 Msa0060940:CDS
TTGTATGTTCTATTGATCTT+TGG 0.316674 1_2:-18920066 Msa0060940:CDS
CATTACTCGACAGTGTTTAG+AGG 0.319594 1_2:-18920167 Msa0060940:CDS
GGTTAAACGTATGATGATAA+TGG 0.326660 1_2:+18919717 None:intergenic
CATTCGAATAATTCTGGAAT+AGG 0.333342 1_2:-18920043 Msa0060940:CDS
GAGGGTGGTCTCTTGTTCAT+TGG 0.339243 1_2:-18919313 Msa0060940:CDS
CACTTGTGATTGAGACTATT+GGG 0.340792 1_2:+18919828 None:intergenic
AAGGTTCCAAATTAAGAGAT+TGG 0.344326 1_2:-18919493 Msa0060940:CDS
CACCGCTGGTGGAATGGTTC+TGG 0.344551 1_2:-18921745 Msa0060940:CDS
GTTAAACGTATGATGATAAT+GGG 0.346709 1_2:+18919718 None:intergenic
TAGCTTCGATCCGGAGGTTT+GGG 0.350467 1_2:+18921138 None:intergenic
CGAACCCTAATAACCGGAAT+TGG 0.360144 1_2:+18922259 None:intergenic
ATGCTACTATATTTCTTCAA+TGG 0.361525 1_2:-18919400 Msa0060940:CDS
CCACCTAGACCTTTAGGGTT+TGG 0.362434 1_2:-18921771 Msa0060940:CDS
ATGCGCAATGGCTTCTTTCT+CGG 0.367067 1_2:+18920963 None:intergenic
TCACTTGTGATTGAGACTAT+TGG 0.368196 1_2:+18919827 None:intergenic
AGAACCAATTCCGGTTATTA+GGG 0.369041 1_2:-18922263 Msa0060940:CDS
CGAAGCGTTTCCGACGTCTA+TGG 0.377428 1_2:-18921925 Msa0060940:CDS
ATAGCTTCGATCCGGAGGTT+TGG 0.378202 1_2:+18921137 None:intergenic
TTCGGTACGTAACAATAGTA+TGG 0.379474 1_2:+18922227 None:intergenic
TTCTAACAGTCTCTCCCATT+TGG 0.383802 1_2:+18919747 None:intergenic
TGCTACTATATTTCTTCAAT+GGG 0.390336 1_2:-18919399 Msa0060940:CDS
ATGACTGAATGTTCTTCAAC+TGG 0.393738 1_2:+18921900 None:intergenic
GCTCACTGTCTCTGCATAAT+GGG 0.397596 1_2:+18919654 None:intergenic
CGAACCTGTTGTTATTATTG+AGG 0.398188 1_2:-18921877 Msa0060940:CDS
GCAATCTGAACTATGCTTCA+TGG 0.399375 1_2:+18920780 None:intergenic
AGACAGAATTATGAGGCTTC+AGG 0.401315 1_2:+18920104 None:intergenic
ATCACTGATATCATTACTTT+CGG 0.403949 1_2:+18921548 None:intergenic
TAACATAATCAATTGTCTTA+GGG 0.407009 1_2:+18921604 None:intergenic
ATTGGTTCTCCTCCTCTGTT+CGG 0.409429 1_2:+18922277 None:intergenic
ATTGCAATATTTACATAACT+TGG 0.409904 1_2:-18920143 Msa0060940:CDS
TAAGAAGCTTGATTTCATCT+AGG 0.411237 1_2:+18920371 None:intergenic
CATCCTCGCATTTCACTCAA+TGG 0.419013 1_2:-18919343 Msa0060940:CDS
TGCCTTCAGGATTTCCAAAT+GGG 0.419108 1_2:-18919761 Msa0060940:CDS
GAGTGAAGAACTCCATGTTC+CGG 0.421411 1_2:-18920665 Msa0060940:CDS
CGATCCGGAGGTTTGGGTAG+TGG 0.425881 1_2:+18921144 None:intergenic
TTTCAACATATCTTTGACAT+TGG 0.427382 1_2:-18919890 Msa0060940:CDS
ACTCTCAAAGTTTCTCTAAA+GGG 0.428966 1_2:-18921719 Msa0060940:CDS
GTTCAGCAGGTTCTTGTAGT+GGG 0.431132 1_2:-18920906 Msa0060940:CDS
ATCCTGAAGGCAAATTGAAC+AGG 0.433788 1_2:+18919772 None:intergenic
TTATTACGAACCCTAATAAC+CGG 0.438088 1_2:+18922253 None:intergenic
GGATGTGACAGAGGCTTACT+AGG 0.444182 1_2:-18919869 Msa0060940:CDS
GGCTTCTTTCTCGGCACTGA+GGG 0.450284 1_2:+18920972 None:intergenic
TCTGGTGGTGCAGGAAGTGG+TGG 0.450710 1_2:+18921066 None:intergenic
ATGACGAGGGCAGAGTTTGC+CGG 0.451565 1_2:-18922409 Msa0060940:CDS
AGGGTCTGATTTAAGAAGGC+TGG 0.452779 1_2:+18921623 None:intergenic
GAGCTTGAAGTGAAATATCT+AGG 0.452943 1_2:+18921793 None:intergenic
GGTGGTCTCTTGTTCATTGG+AGG 0.455783 1_2:-18919310 Msa0060940:CDS
TATTTCAACAAACATGTAAT+GGG 0.455848 1_2:+18920996 None:intergenic
CAGAGGAGGAGAACCAATTC+CGG 0.457954 1_2:-18922272 Msa0060940:CDS
GTCTCCTCAATAATAACAAC+AGG 0.458553 1_2:+18921873 None:intergenic
CATACATGAGTTAGTAAAGA+TGG 0.460709 1_2:-18921487 Msa0060940:CDS
TTATGACTATTTCTACCATC+AGG 0.460782 1_2:-18920299 Msa0060940:CDS
TCTCCATCTGTGGCAGCCTC+CGG 0.462617 1_2:+18920646 None:intergenic
TGAACAAGAGACCACCCTCC+TGG 0.466679 1_2:+18919317 None:intergenic
TTGCCTTCAGGATTTCCAAA+TGG 0.467728 1_2:-18919762 Msa0060940:CDS
CTACAAGAACCTGCTGAACC+TGG 0.473048 1_2:+18920910 None:intergenic
AACAAAGAAATATCAGATAG+TGG 0.475294 1_2:-18921831 Msa0060940:CDS
GTGTCTGGTGGTGCAGGAAG+TGG 0.476291 1_2:+18921063 None:intergenic
TCTTAGGGTCTGATTTAAGA+AGG 0.478323 1_2:+18921619 None:intergenic
TTGGGTAGTGGCGGCAGAGT+CGG 0.481200 1_2:+18921156 None:intergenic
AATTCTTACGCAATTGAAAG+AGG 0.482578 1_2:-18921970 Msa0060940:CDS
TAAGTTTGCACGCAGAAGTT+CGG 0.485105 1_2:+18920258 None:intergenic
GGTAAGAGTCGCGGTGATGC+CGG 0.490474 1_2:+18922390 None:intergenic
TGGCAACACCGCTGGTGGAA+TGG 0.491231 1_2:-18921751 Msa0060940:CDS
ATCCTCGCATTTCACTCAAT+GGG 0.493681 1_2:-18919342 Msa0060940:CDS
GTCAATTTGACGAGCTGCAA+AGG 0.493930 1_2:-18921270 Msa0060940:CDS
TGATATTTCTTTGTTTAGCA+CGG 0.494833 1_2:+18921839 None:intergenic
AAAAGCAGTCCAGGTTCAGC+AGG 0.497375 1_2:-18920919 Msa0060940:CDS
AAGAGGAGATCGACGTTGCT+CGG 0.499224 1_2:-18921953 Msa0060940:CDS
TTGCCAAACCCTAAAGGTCT+AGG 0.500192 1_2:+18921768 None:intergenic
CACTTAGTTGTGTGCAAGTG+TGG 0.500438 1_2:-18919563 Msa0060940:CDS
CTGTGGCAGCCTCCGGAACA+TGG 0.502526 1_2:+18920653 None:intergenic
CATTTCACTCAATGGGATCC+AGG 0.504780 1_2:-18919335 Msa0060940:CDS
GGTTCAGCAGGTTCTTGTAG+TGG 0.505056 1_2:-18920907 Msa0060940:CDS
TTGCAATATTTACATAACTT+GGG 0.506178 1_2:-18920142 Msa0060940:CDS
AGTATGTGGCGTTTATCTCC+CGG 0.506715 1_2:+18920328 None:intergenic
TGAACTTTCTATTGCAGCAT+CGG 0.506969 1_2:-18921520 Msa0060940:CDS
TTAGGGTTTGGCAACACCGC+TGG 0.507478 1_2:-18921759 Msa0060940:CDS
CATAGACGTCGGAAACGCTT+CGG 0.508149 1_2:+18921926 None:intergenic
GTTCCGGAGGCTGCCACAGA+TGG 0.508408 1_2:-18920649 Msa0060940:CDS
ATCTTCTGATAGCTTCGATC+CGG 0.508560 1_2:+18921129 None:intergenic
ATAGAGAAGACAGAATTATG+AGG 0.508809 1_2:+18920097 None:intergenic
CACTCTCAAAGTTTCTCTAA+AGG 0.509641 1_2:-18921720 Msa0060940:CDS
CACACAACTAAGTGTAGGCT+TGG 0.511853 1_2:+18919572 None:intergenic
GTATTTCAACAAACATGTAA+TGG 0.512226 1_2:+18920995 None:intergenic
CCATTGCTTTCAATCCCATT+CGG 0.516665 1_2:+18920709 None:intergenic
ACCTACCTTGCATTGACAAG+TGG 0.517649 1_2:-18919697 Msa0060940:CDS
ACCAGACACCGAAAATAGAT+TGG 0.519369 1_2:-18921049 Msa0060940:CDS
GGAGTTCTTCACTCAAATAT+CGG 0.520249 1_2:+18920674 None:intergenic
GATGTCGGCGTGGACAACTA+AGG 0.529317 1_2:-18922341 Msa0060940:CDS
ATCTGTTTCAACAACTTCGA+TGG 0.530198 1_2:+18920735 None:intergenic
CATACCAGAAAGATGAATCA+AGG 0.532240 1_2:+18921245 None:intergenic
CGTCATCAGTTGATCTCCCA+TGG 0.534348 1_2:+18922426 None:intergenic
GAGGTGGAAGAGACTGATGC+AGG 0.534361 1_2:-18919230 Msa0060940:CDS
TTGAAACAGATCTACCGAAT+GGG 0.535513 1_2:-18920723 Msa0060940:CDS
TAAAGGAAATTAAGCTGAGT+CGG 0.536864 1_2:-18920855 Msa0060940:CDS
CTAGGCTACCTCTAGAACAA+TGG 0.538104 1_2:-18919445 Msa0060940:CDS
GGTGGTGCAGGAAGTGGTGG+AGG 0.539230 1_2:+18921069 None:intergenic
TCGGTTCAGCTACCTTCAGT+AGG 0.540246 1_2:-18920453 Msa0060940:CDS
TTCTGATAGCTTCGATCCGG+AGG 0.545370 1_2:+18921132 None:intergenic
GATTGTGCTGAACACGGTCA+TGG 0.546919 1_2:-18920503 Msa0060940:CDS
TATCAGATAGTGGAGCTAAC+GGG 0.549949 1_2:-18921821 Msa0060940:CDS
GTGCATGGAAAGAATAGGAC+TGG 0.553009 1_2:-18920550 Msa0060940:CDS
ATATCAGATAGTGGAGCTAA+CGG 0.555900 1_2:-18921822 Msa0060940:CDS
GTATTATTGTGAAAGACTCT+AGG 0.560834 1_2:-18919463 Msa0060940:CDS
GCCGCCACTACCCAAACCTC+CGG 0.562559 1_2:-18921148 Msa0060940:CDS
GGGTTTGGCAACACCGCTGG+TGG 0.563437 1_2:-18921756 Msa0060940:CDS
AACTTCAGAATTATGCGCAA+TGG 0.563755 1_2:+18920951 None:intergenic
GTATGTGGCGTTTATCTCCC+GGG 0.564712 1_2:+18920329 None:intergenic
TTCACCATCCATTGTTCTAG+AGG 0.564813 1_2:+18919437 None:intergenic
TTCAGCAGGTTCTTGTAGTG+GGG 0.568017 1_2:-18920905 Msa0060940:CDS
GCGGTGTTGCCAAACCCTAA+AGG 0.568245 1_2:+18921762 None:intergenic
CTCTCAAAGTTTCTCTAAAG+GGG 0.571073 1_2:-18921718 Msa0060940:CDS
GTACAAGTCTCCCACTCACT+AGG 0.571667 1_2:+18919987 None:intergenic
AGTCATAAAGACGCAGTATG+TGG 0.573921 1_2:+18920314 None:intergenic
TGCAGGAAGTGGTGGAGGCA+AGG 0.579538 1_2:+18921074 None:intergenic
GACAAGTGGCTCATCAAGCA+TGG 0.579677 1_2:-18919683 Msa0060940:CDS
CTCCCATTTGGAAATCCTGA+AGG 0.579740 1_2:+18919759 None:intergenic
GAATTGTGCATGGAAAGAAT+AGG 0.580039 1_2:-18920555 Msa0060940:CDS
TTGGAGGAGTAAACATGCAT+GGG 0.580232 1_2:-18919294 Msa0060940:CDS
TTCAGTAGGGATGCTCTCGA+TGG 0.583985 1_2:-18920439 Msa0060940:CDS
ATTGGAGGAGTAAACATGCA+TGG 0.584144 1_2:-18919295 Msa0060940:CDS
CGGTTCAGCTACCTTCAGTA+GGG 0.590898 1_2:-18920452 Msa0060940:CDS
CTTTAGAGAAACTTTGAGAG+TGG 0.596298 1_2:+18921721 None:intergenic
GTTCAAAGTCAGATGGGTGC+AGG 0.598923 1_2:-18919932 Msa0060940:CDS
AAAAGCAAGCACTTAATGGA+TGG 0.599900 1_2:-18919526 Msa0060940:CDS
CAATGAATAAATGTTCCTGA+TGG 0.601057 1_2:+18920284 None:intergenic
GAATGCTGTTCAAAGTCAGA+TGG 0.601226 1_2:-18919939 Msa0060940:CDS
GGGATTGAAAGCAATGGAGT+AGG 0.601533 1_2:-18920703 Msa0060940:CDS
TTATTACTAGCCTAGTGAGT+GGG 0.602053 1_2:-18919997 Msa0060940:CDS
ATAAAAGTGAATGTAGCAGA+TGG 0.603482 1_2:-18921654 Msa0060940:CDS
TGTGAATGTTTCGATAGTAG+TGG 0.604151 1_2:+18921195 None:intergenic
GGTGAAATTCATCACAACAT+CGG 0.604562 1_2:-18921028 Msa0060940:CDS
TCCGGAGGTTTGGGTAGTGG+CGG 0.605445 1_2:+18921147 None:intergenic
ATGAGCCACTTGTCAATGCA+AGG 0.605600 1_2:+18919692 None:intergenic
GTTGAAACAGATCTACCGAA+TGG 0.607989 1_2:-18920724 Msa0060940:CDS
CCGAATGGGATTGAAAGCAA+TGG 0.608264 1_2:-18920709 Msa0060940:CDS
TTTGACATTGGATGTGACAG+AGG 0.609823 1_2:-18919878 Msa0060940:CDS
TTTGAGCTAAGAATTGTGCA+TGG 0.611242 1_2:-18920565 Msa0060940:CDS
CATCACCGCGACTCTTACCA+CGG 0.611655 1_2:-18922386 Msa0060940:CDS
GACTGGATTTGAAGAAAACA+AGG 0.612197 1_2:-18920533 Msa0060940:CDS
TGGCTTCTTTCTCGGCACTG+AGG 0.612609 1_2:+18920971 None:intergenic
ATCGAGAGCATCCCTACTGA+AGG 0.612994 1_2:+18920441 None:intergenic
CTCAATGGGATCCAGGAGGG+TGG 0.615498 1_2:-18919328 Msa0060940:CDS
CTCGAGGTCGAGTTCTTCAG+AGG 0.615630 1_2:-18919249 Msa0060940:CDS
GCATAGTTCAGATTGCTACA+AGG 0.615746 1_2:-18920773 Msa0060940:CDS
TCACTCAATGGGATCCAGGA+GGG 0.616384 1_2:-18919331 Msa0060940:CDS
AGAAACAATCCGAACAGAGG+AGG 0.617511 1_2:-18922286 Msa0060940:CDS
TACTATGTGACAGATTATCT+CGG 0.618368 1_2:-18920472 Msa0060940:CDS
ATTATTATCCTCTCCATCTG+TGG 0.619270 1_2:+18920636 None:intergenic
TGAACACGGTCATGGCTGAA+AGG 0.620232 1_2:-18920495 Msa0060940:CDS
TTTATCAAAATGAGAACTCG+AGG 0.621879 1_2:-18919265 Msa0060940:CDS
GTGGAAGAGACTGATGCAGG+AGG 0.622444 1_2:-18919227 Msa0060940:CDS
ACTTTCTATTGCAGCATCGG+AGG 0.622968 1_2:-18921517 Msa0060940:CDS
TCTAGAACAATGGATGGTGA+AGG 0.627891 1_2:-18919435 Msa0060940:CDS
CTTGTCGATAAGCACGACCC+GGG 0.628203 1_2:-18920346 Msa0060940:CDS
GTCCAGAACCATTCCACCAG+CGG 0.631736 1_2:+18921743 None:intergenic
TGGAAGAGACTGATGCAGGA+GGG 0.637702 1_2:-18919226 Msa0060940:CDS
ACTTGCACACAACTAAGTGT+AGG 0.640898 1_2:+18919567 None:intergenic
TCTCAAAGTTTCTCTAAAGG+GGG 0.641090 1_2:-18921717 Msa0060940:CDS
AACAGAAACAATCCGAACAG+AGG 0.642385 1_2:-18922289 Msa0060940:CDS
TAACAGAGCTTTGATGTCCG+TGG 0.643055 1_2:+18922369 None:intergenic
TGAGTTAGTAAAGATGGAGT+CGG 0.643279 1_2:-18921481 Msa0060940:CDS
ACTTTGAACAGCATTCAACA+TGG 0.643300 1_2:+18919946 None:intergenic
GGAGGCTGCCACAGATGGAG+AGG 0.645603 1_2:-18920644 Msa0060940:CDS
TGGGAGATCAACTGATGACG+AGG 0.645863 1_2:-18922423 Msa0060940:CDS
GCTACCTCTAGAACAATGGA+TGG 0.646462 1_2:-18919441 Msa0060940:CDS
AGCTCTGTTAGATCAGATGT+CGG 0.647374 1_2:-18922356 Msa0060940:CDS
GAGGTCGAGTTCTTCAGAGG+TGG 0.650920 1_2:-18919246 Msa0060940:CDS
AAGCTATCAGAAGATTCTCG+TGG 0.653064 1_2:-18921121 Msa0060940:CDS
ATTATTACTAGCCTAGTGAG+TGG 0.653310 1_2:-18919998 Msa0060940:CDS
TGAAGAACTCCATGTTCCGG+AGG 0.656619 1_2:-18920662 Msa0060940:CDS
AATGCTGTTCAAAGTCAGAT+GGG 0.656650 1_2:-18919938 Msa0060940:CDS
ACTTGTCGATAAGCACGACC+CGG 0.656700 1_2:-18920347 Msa0060940:CDS
TCAACTGGTTCCATAGACGT+CGG 0.662770 1_2:+18921915 None:intergenic
GGGAGATCAACTGATGACGA+GGG 0.662978 1_2:-18922422 Msa0060940:CDS
GCTTCTTTCTCGGCACTGAG+GGG 0.663612 1_2:+18920973 None:intergenic
CCAAACCCTAAAGGTCTAGG+TGG 0.663816 1_2:+18921771 None:intergenic
GATGTCCGTGGTAAGAGTCG+CGG 0.666618 1_2:+18922381 None:intergenic
AAATTCAACCAAGAATATGG+TGG 0.668921 1_2:-18920223 Msa0060940:CDS
TTAAACGTATGATGATAATG+GGG 0.679275 1_2:+18919719 None:intergenic
AGACTTGTACGTGAAAGAGT+TGG 0.691935 1_2:-18919974 Msa0060940:CDS
GCCACTTGTCAATGCAAGGT+AGG 0.694066 1_2:+18919696 None:intergenic
AATGGATGGCAACAACACAA+AGG 0.695166 1_2:-18919512 Msa0060940:CDS
CAGATAGTGGAGCTAACGGG+AGG 0.697790 1_2:-18921818 Msa0060940:CDS
TTCACTCAATGGGATCCAGG+AGG 0.704558 1_2:-18919332 Msa0060940:CDS
TGTTAGATCAGATGTCGGCG+TGG 0.711421 1_2:-18922351 Msa0060940:CDS
AGTGCTGATTGTGCTGAACA+CGG 0.717577 1_2:-18920509 Msa0060940:CDS
AGAGTCGGAGTGAAAACACG+TGG 0.744559 1_2:+18921171 None:intergenic
ATCCCATTGAGTGAAATGCG+AGG 0.759058 1_2:+18919340 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATAATACAACAAATTGA+CGG - chr1_2:18919480-18919499 Msa0060940:CDS 20.0%
!!! TAATCTTTGTTGTAACAAAA+CGG + chr1_2:18919550-18919569 None:intergenic 20.0%
!! TAACATAATCAATTGTCTTA+GGG + chr1_2:18920040-18920059 None:intergenic 20.0%
!! TTAACATAATCAATTGTCTT+AGG + chr1_2:18920041-18920060 None:intergenic 20.0%
!! TATTTCAACAAACATGTAAT+GGG + chr1_2:18920648-18920667 None:intergenic 20.0%
!! GCTTAATTTCCTTTAAATTA+AGG + chr1_2:18920781-18920800 None:intergenic 20.0%
!! GAAGATTATTAAAAATGACA+AGG - chr1_2:18921231-18921250 Msa0060940:CDS 20.0%
!! ATTGCAATATTTACATAACT+TGG - chr1_2:18921498-18921517 Msa0060940:CDS 20.0%
!! TTGCAATATTTACATAACTT+GGG - chr1_2:18921499-18921518 Msa0060940:CDS 20.0%
!!! AAGATTAATGTTTTTTGCAA+TGG - chr1_2:18922027-18922046 Msa0060940:intron 20.0%
!! TGATATTTCTTTGTTTAGCA+CGG + chr1_2:18919805-18919824 None:intergenic 25.0%
! AACAAAGAAATATCAGATAG+TGG - chr1_2:18919810-18919829 Msa0060940:CDS 25.0%
! ACAACAGAAAGAAAAATCAA+AGG - chr1_2:18920061-18920080 Msa0060940:CDS 25.0%
! ATCACTGATATCATTACTTT+CGG + chr1_2:18920096-18920115 None:intergenic 25.0%
!!! TCTGATGAATCTTTAGTTTT+TGG + chr1_2:18920224-18920243 None:intergenic 25.0%
!! GAATTTCACCAATCTATTTT+CGG + chr1_2:18920603-18920622 None:intergenic 25.0%
! GTATTTCAACAAACATGTAA+TGG + chr1_2:18920649-18920668 None:intergenic 25.0%
! TTAAGGTAGCTAATGTTTAT+AGG + chr1_2:18920764-18920783 None:intergenic 25.0%
! CATTAGCTACCTTAATTTAA+AGG - chr1_2:18920769-18920788 Msa0060940:CDS 25.0%
! CATAAATTCAACCAAGAATA+TGG - chr1_2:18921415-18921434 Msa0060940:intron 25.0%
! TTGTATGTTCTATTGATCTT+TGG - chr1_2:18921575-18921594 Msa0060940:CDS 25.0%
! TTTCAACATATCTTTGACAT+TGG - chr1_2:18921751-18921770 Msa0060940:CDS 25.0%
!! TTAAACGTATGATGATAATG+GGG + chr1_2:18921925-18921944 None:intergenic 25.0%
! GTTAAACGTATGATGATAAT+GGG + chr1_2:18921926-18921945 None:intergenic 25.0%
!! TGTTTTCCAATCTCTTAATT+TGG + chr1_2:18922157-18922176 None:intergenic 25.0%
! ATGCTACTATATTTCTTCAA+TGG - chr1_2:18922241-18922260 Msa0060940:CDS 25.0%
! TGCTACTATATTTCTTCAAT+GGG - chr1_2:18922242-18922261 Msa0060940:CDS 25.0%
TTATTACGAACCCTAATAAC+CGG + chr1_2:18919391-18919410 None:intergenic 30.0%
!!! CACAGGTATATTTTGACTTT+CGG + chr1_2:18919435-18919454 None:intergenic 30.0%
ATATACCTGTGAATCTGATT+TGG - chr1_2:18919444-18919463 Msa0060940:CDS 30.0%
AATTCTTACGCAATTGAAAG+AGG - chr1_2:18919671-18919690 Msa0060940:CDS 30.0%
ACTCTCAAAGTTTCTCTAAA+GGG - chr1_2:18919922-18919941 Msa0060940:CDS 30.0%
ATAAAAGTGAATGTAGCAGA+TGG - chr1_2:18919987-18920006 Msa0060940:CDS 30.0%
CATACATGAGTTAGTAAAGA+TGG - chr1_2:18920154-18920173 Msa0060940:CDS 30.0%
ATTAAAGTGTACTCAAGTGA+AGG - chr1_2:18920251-18920270 Msa0060940:CDS 30.0%
TTAAAGTGTACTCAAGTGAA+GGG - chr1_2:18920252-18920271 Msa0060940:CDS 30.0%
TAAAGGAAATTAAGCTGAGT+CGG - chr1_2:18920786-18920805 Msa0060940:CDS 30.0%
! TCGGAATGATTTTCATATGA+AGG - chr1_2:18920805-18920824 Msa0060940:CDS 30.0%
! CGGAATGATTTTCATATGAA+GGG - chr1_2:18920806-18920825 Msa0060940:CDS 30.0%
!! CATCTTGACTGTATTTTAAG+AGG + chr1_2:18921042-18921061 None:intergenic 30.0%
TACTATGTGACAGATTATCT+CGG - chr1_2:18921169-18921188 Msa0060940:CDS 30.0%
TAAGAAGCTTGATTTCATCT+AGG + chr1_2:18921273-18921292 None:intergenic 30.0%
!! TTATGACTATTTCTACCATC+AGG - chr1_2:18921342-18921361 Msa0060940:intron 30.0%
CAATGAATAAATGTTCCTGA+TGG + chr1_2:18921360-18921379 None:intergenic 30.0%
AAATTCAACCAAGAATATGG+TGG - chr1_2:18921418-18921437 Msa0060940:CDS 30.0%
ATAGAGAAGACAGAATTATG+AGG + chr1_2:18921547-18921566 None:intergenic 30.0%
!! CATTCGAATAATTCTGGAAT+AGG - chr1_2:18921598-18921617 Msa0060940:CDS 30.0%
GGTTAAACGTATGATGATAA+TGG + chr1_2:18921927-18921946 None:intergenic 30.0%
AAGGTTCCAAATTAAGAGAT+TGG - chr1_2:18922148-18922167 Msa0060940:intron 30.0%
GTATTATTGTGAAAGACTCT+AGG - chr1_2:18922178-18922197 Msa0060940:intron 30.0%
TTTATCAAAATGAGAACTCG+AGG - chr1_2:18922376-18922395 Msa0060940:CDS 30.0%
! GTTTCTGTTGTTGTTGTTGT+TGG + chr1_2:18919341-18919360 None:intergenic 35.0%
AGAACCAATTCCGGTTATTA+GGG - chr1_2:18919378-18919397 Msa0060940:CDS 35.0%
TTCGGTACGTAACAATAGTA+TGG + chr1_2:18919417-18919436 None:intergenic 35.0%
GTATTCCAAATCAGATTCAC+AGG + chr1_2:18919452-18919471 None:intergenic 35.0%
AATAGCAGATAACTCACGTA+AGG - chr1_2:18919603-18919622 Msa0060940:CDS 35.0%
ATGACTGAATGTTCTTCAAC+TGG + chr1_2:18919744-18919763 None:intergenic 35.0%
! CGAACCTGTTGTTATTATTG+AGG - chr1_2:18919764-18919783 Msa0060940:CDS 35.0%
GTCTCCTCAATAATAACAAC+AGG + chr1_2:18919771-18919790 None:intergenic 35.0%
!! ATATCAGATAGTGGAGCTAA+CGG - chr1_2:18919819-18919838 Msa0060940:CDS 35.0%
GAGCTTGAAGTGAAATATCT+AGG + chr1_2:18919851-18919870 None:intergenic 35.0%
! CTTTAGAGAAACTTTGAGAG+TGG + chr1_2:18919923-18919942 None:intergenic 35.0%
CACTCTCAAAGTTTCTCTAA+AGG - chr1_2:18919921-18919940 Msa0060940:CDS 35.0%
! CTCTCAAAGTTTCTCTAAAG+GGG - chr1_2:18919923-18919942 Msa0060940:CDS 35.0%
! TCTCAAAGTTTCTCTAAAGG+GGG - chr1_2:18919924-18919943 Msa0060940:CDS 35.0%
TCTTAGGGTCTGATTTAAGA+AGG + chr1_2:18920025-18920044 None:intergenic 35.0%
TGAACTTTCTATTGCAGCAT+CGG - chr1_2:18920121-18920140 Msa0060940:CDS 35.0%
TGAGTTAGTAAAGATGGAGT+CGG - chr1_2:18920160-18920179 Msa0060940:CDS 35.0%
TAAAGTGTACTCAAGTGAAG+GGG - chr1_2:18920253-18920272 Msa0060940:CDS 35.0%
CATACCAGAAAGATGAATCA+AGG + chr1_2:18920399-18920418 None:intergenic 35.0%
TGTGAATGTTTCGATAGTAG+TGG + chr1_2:18920449-18920468 None:intergenic 35.0%
GGTGAAATTCATCACAACAT+CGG - chr1_2:18920613-18920632 Msa0060940:CDS 35.0%
AACTTCAGAATTATGCGCAA+TGG + chr1_2:18920693-18920712 None:intergenic 35.0%
ATCTGTTTCAACAACTTCGA+TGG + chr1_2:18920909-18920928 None:intergenic 35.0%
TTGAAACAGATCTACCGAAT+GGG - chr1_2:18920918-18920937 Msa0060940:CDS 35.0%
GGAGTTCTTCACTCAAATAT+CGG + chr1_2:18920970-18920989 None:intergenic 35.0%
ATTATTATCCTCTCCATCTG+TGG + chr1_2:18921008-18921027 None:intergenic 35.0%
TTTGAGCTAAGAATTGTGCA+TGG - chr1_2:18921076-18921095 Msa0060940:CDS 35.0%
GAATTGTGCATGGAAAGAAT+AGG - chr1_2:18921086-18921105 Msa0060940:CDS 35.0%
GACTGGATTTGAAGAAAACA+AGG - chr1_2:18921108-18921127 Msa0060940:CDS 35.0%
CTTTGGCATTCGAATAATTC+TGG - chr1_2:18921592-18921611 Msa0060940:CDS 35.0%
! ATTATTACTAGCCTAGTGAG+TGG - chr1_2:18921643-18921662 Msa0060940:CDS 35.0%
! TTATTACTAGCCTAGTGAGT+GGG - chr1_2:18921644-18921663 Msa0060940:CDS 35.0%
ACTTTGAACAGCATTCAACA+TGG + chr1_2:18921698-18921717 None:intergenic 35.0%
AATGCTGTTCAAAGTCAGAT+GGG - chr1_2:18921703-18921722 Msa0060940:CDS 35.0%
CACTTGTGATTGAGACTATT+GGG + chr1_2:18921816-18921835 None:intergenic 35.0%
TCACTTGTGATTGAGACTAT+TGG + chr1_2:18921817-18921836 None:intergenic 35.0%
!! GACAACAATTTTGATGCTTG+TGG - chr1_2:18921835-18921854 Msa0060940:CDS 35.0%
ACAGAAAAGCAAGCACTTAA+TGG - chr1_2:18922111-18922130 Msa0060940:intron 35.0%
AAAAGCAAGCACTTAATGGA+TGG - chr1_2:18922115-18922134 Msa0060940:intron 35.0%
AGCTCTGTTAGATCAGATGT+CGG - chr1_2:18919285-18919304 Msa0060940:CDS 40.0%
AACAGAAACAATCCGAACAG+AGG - chr1_2:18919352-18919371 Msa0060940:CDS 40.0%
GAGAACCAATTCCGGTTATT+AGG - chr1_2:18919377-18919396 Msa0060940:CDS 40.0%
CGGACAACGATATATCGAAT+CGG + chr1_2:18919530-18919549 None:intergenic 40.0%
!! TATCAGATAGTGGAGCTAAC+GGG - chr1_2:18919820-18919839 Msa0060940:CDS 40.0%
! ATCACCAAAAGGCTTTTTGC+AGG - chr1_2:18919950-18919969 Msa0060940:CDS 40.0%
! AAAGCCTGCAAAAAGCCTTT+TGG + chr1_2:18919957-18919976 None:intergenic 40.0%
! TAGCCTACTCTTTTGTGTCA+TGG - chr1_2:18920284-18920303 Msa0060940:CDS 40.0%
GGCTCAGGTGTATTCTATAA+CGG - chr1_2:18920305-18920324 Msa0060940:CDS 40.0%
! ACCACCTGAAGTGCTAAATA+CGG + chr1_2:18920353-18920372 None:intergenic 40.0%
GGATCCTTGATTCATCTTTC+TGG - chr1_2:18920392-18920411 Msa0060940:CDS 40.0%
ATCTTCTGATAGCTTCGATC+CGG + chr1_2:18920515-18920534 None:intergenic 40.0%
AAGCTATCAGAAGATTCTCG+TGG - chr1_2:18920520-18920539 Msa0060940:CDS 40.0%
!!! AATCTATTTTCGGTGTCTGG+TGG + chr1_2:18920593-18920612 None:intergenic 40.0%
ACCAGACACCGAAAATAGAT+TGG - chr1_2:18920592-18920611 Msa0060940:CDS 40.0%
!!! ACCAATCTATTTTCGGTGTC+TGG + chr1_2:18920596-18920615 None:intergenic 40.0%
GCAATCTGAACTATGCTTCA+TGG + chr1_2:18920864-18920883 None:intergenic 40.0%
GCATAGTTCAGATTGCTACA+AGG - chr1_2:18920868-18920887 Msa0060940:CDS 40.0%
GTTGAAACAGATCTACCGAA+TGG - chr1_2:18920917-18920936 Msa0060940:CDS 40.0%
CCATTGCTTTCAATCCCATT+CGG + chr1_2:18920935-18920954 None:intergenic 40.0%
AGTCATAAAGACGCAGTATG+TGG + chr1_2:18921330-18921349 None:intergenic 40.0%
TAAGTTTGCACGCAGAAGTT+CGG + chr1_2:18921386-18921405 None:intergenic 40.0%
ATGCTTCACCACCATATTCT+TGG + chr1_2:18921429-18921448 None:intergenic 40.0%
CATTACTCGACAGTGTTTAG+AGG - chr1_2:18921474-18921493 Msa0060940:CDS 40.0%
AGACAGAATTATGAGGCTTC+AGG + chr1_2:18921540-18921559 None:intergenic 40.0%
AGACTTGTACGTGAAAGAGT+TGG - chr1_2:18921667-18921686 Msa0060940:CDS 40.0%
GAATGCTGTTCAAAGTCAGA+TGG - chr1_2:18921702-18921721 Msa0060940:CDS 40.0%
TTTGACATTGGATGTGACAG+AGG - chr1_2:18921763-18921782 Msa0060940:CDS 40.0%
TTCCTGTTCAATTTGCCTTC+AGG - chr1_2:18921867-18921886 Msa0060940:CDS 40.0%
ATCCTGAAGGCAAATTGAAC+AGG + chr1_2:18921872-18921891 None:intergenic 40.0%
TTGCCTTCAGGATTTCCAAA+TGG - chr1_2:18921879-18921898 Msa0060940:CDS 40.0%
TGCCTTCAGGATTTCCAAAT+GGG - chr1_2:18921880-18921899 Msa0060940:CDS 40.0%
TTCTAACAGTCTCTCCCATT+TGG + chr1_2:18921897-18921916 None:intergenic 40.0%
ACTTGCACACAACTAAGTGT+AGG + chr1_2:18922077-18922096 None:intergenic 40.0%
! TGTGCAAGTGTGGTTTTTGT+GGG - chr1_2:18922088-18922107 Msa0060940:intron 40.0%
AATGGATGGCAACAACACAA+AGG - chr1_2:18922129-18922148 Msa0060940:intron 40.0%
TTCACCATCCATTGTTCTAG+AGG + chr1_2:18922207-18922226 None:intergenic 40.0%
! TCTAGAACAATGGATGGTGA+AGG - chr1_2:18922206-18922225 Msa0060940:intron 40.0%
ATCCTCGCATTTCACTCAAT+GGG - chr1_2:18922299-18922318 Msa0060940:CDS 40.0%
ATTGGAGGAGTAAACATGCA+TGG - chr1_2:18922346-18922365 Msa0060940:CDS 40.0%
TTGGAGGAGTAAACATGCAT+GGG - chr1_2:18922347-18922366 Msa0060940:CDS 40.0%
! TAACAGAGCTTTGATGTCCG+TGG + chr1_2:18919275-18919294 None:intergenic 45.0%
AGAAACAATCCGAACAGAGG+AGG - chr1_2:18919355-18919374 Msa0060940:CDS 45.0%
! ATTGGTTCTCCTCCTCTGTT+CGG + chr1_2:18919367-18919386 None:intergenic 45.0%
CGAACCCTAATAACCGGAAT+TGG + chr1_2:18919385-18919404 None:intergenic 45.0%
GTGTCTCAAAAGGTCAGTCA+AGG + chr1_2:18919575-18919594 None:intergenic 45.0%
! TTCCAGAAGCGTGTCTCAAA+AGG + chr1_2:18919585-18919604 None:intergenic 45.0%
! TCAACTGGTTCCATAGACGT+CGG + chr1_2:18919729-18919748 None:intergenic 45.0%
AAGCTCCACCTAGACCTTTA+GGG - chr1_2:18919865-18919884 Msa0060940:CDS 45.0%
TTGCCAAACCCTAAAGGTCT+AGG + chr1_2:18919876-18919895 None:intergenic 45.0%
AAAGGGGGATCATCACCAAA+AGG - chr1_2:18919939-18919958 Msa0060940:CDS 45.0%
AGGGTCTGATTTAAGAAGGC+TGG + chr1_2:18920021-18920040 None:intergenic 45.0%
! ACTTTCTATTGCAGCATCGG+AGG - chr1_2:18920124-18920143 Msa0060940:CDS 45.0%
GAGCCATGACACAAAAGAGT+AGG + chr1_2:18920290-18920309 None:intergenic 45.0%
! ACTCTTTTGTGTCATGGCTC+AGG - chr1_2:18920290-18920309 Msa0060940:CDS 45.0%
CACACCGTATTTAGCACTTC+AGG - chr1_2:18920346-18920365 Msa0060940:CDS 45.0%
ACCGTATTTAGCACTTCAGG+TGG - chr1_2:18920349-18920368 Msa0060940:CDS 45.0%
GTCAATTTGACGAGCTGCAA+AGG - chr1_2:18920371-18920390 Msa0060940:CDS 45.0%
ATGCGCAATGGCTTCTTTCT+CGG + chr1_2:18920681-18920700 None:intergenic 45.0%
!! CCTGGACTGCTTTTGCAAAA+TGG + chr1_2:18920716-18920735 None:intergenic 45.0%
! CCATTTTGCAAAAGCAGTCC+AGG - chr1_2:18920713-18920732 Msa0060940:CDS 45.0%
GTTCAGCAGGTTCTTGTAGT+GGG - chr1_2:18920735-18920754 Msa0060940:CDS 45.0%
TTCAGCAGGTTCTTGTAGTG+GGG - chr1_2:18920736-18920755 Msa0060940:CDS 45.0%
CCGAATGGGATTGAAAGCAA+TGG - chr1_2:18920932-18920951 Msa0060940:CDS 45.0%
GGGATTGAAAGCAATGGAGT+AGG - chr1_2:18920938-18920957 Msa0060940:CDS 45.0%
GAGTGAAGAACTCCATGTTC+CGG - chr1_2:18920976-18920995 Msa0060940:CDS 45.0%
GTGCATGGAAAGAATAGGAC+TGG - chr1_2:18921091-18921110 Msa0060940:CDS 45.0%
!! AGTGCTGATTGTGCTGAACA+CGG - chr1_2:18921132-18921151 Msa0060940:CDS 45.0%
AGTATGTGGCGTTTATCTCC+CGG + chr1_2:18921316-18921335 None:intergenic 45.0%
CTCCCATTTGGAAATCCTGA+AGG + chr1_2:18921885-18921904 None:intergenic 45.0%
! ACCTACCTTGCATTGACAAG+TGG - chr1_2:18921944-18921963 Msa0060940:CDS 45.0%
ATGAGCCACTTGTCAATGCA+AGG + chr1_2:18921952-18921971 None:intergenic 45.0%
GCTCACTGTCTCTGCATAAT+GGG + chr1_2:18921990-18922009 None:intergenic 45.0%
TGCTCACTGTCTCTGCATAA+TGG + chr1_2:18921991-18922010 None:intergenic 45.0%
CACACAACTAAGTGTAGGCT+TGG + chr1_2:18922072-18922091 None:intergenic 45.0%
CACTTAGTTGTGTGCAAGTG+TGG - chr1_2:18922078-18922097 Msa0060940:intron 45.0%
! GTGTGCAAGTGTGGTTTTTG+TGG - chr1_2:18922087-18922106 Msa0060940:intron 45.0%
!! CTAGGCTACCTCTAGAACAA+TGG - chr1_2:18922196-18922215 Msa0060940:intron 45.0%
!! GCTACCTCTAGAACAATGGA+TGG - chr1_2:18922200-18922219 Msa0060940:intron 45.0%
CATCCTCGCATTTCACTCAA+TGG - chr1_2:18922298-18922317 Msa0060940:CDS 45.0%
ATCCCATTGAGTGAAATGCG+AGG + chr1_2:18922304-18922323 None:intergenic 45.0%
CATTTCACTCAATGGGATCC+AGG - chr1_2:18922306-18922325 Msa0060940:CDS 45.0%
! TGGGAGATCAACTGATGACG+AGG - chr1_2:18919218-18919237 Msa0060940:CDS 50.0%
! GGGAGATCAACTGATGACGA+GGG - chr1_2:18919219-18919238 Msa0060940:CDS 50.0%
TGTTAGATCAGATGTCGGCG+TGG - chr1_2:18919290-18919309 Msa0060940:CDS 50.0%
CAGAGGAGGAGAACCAATTC+CGG - chr1_2:18919369-18919388 Msa0060940:CDS 50.0%
!!! GACCTTTTGAGACACGCTTC+TGG - chr1_2:18919580-18919599 Msa0060940:CDS 50.0%
AAGAGGAGATCGACGTTGCT+CGG - chr1_2:18919688-18919707 Msa0060940:CDS 50.0%
CATAGACGTCGGAAACGCTT+CGG + chr1_2:18919718-18919737 None:intergenic 50.0%
CAAGCTCCACCTAGACCTTT+AGG - chr1_2:18919864-18919883 Msa0060940:CDS 50.0%
CCAAACCCTAAAGGTCTAGG+TGG + chr1_2:18919873-18919892 None:intergenic 50.0%
!! CCACCTAGACCTTTAGGGTT+TGG - chr1_2:18919870-18919889 Msa0060940:CDS 50.0%
AGAGTCGGAGTGAAAACACG+TGG + chr1_2:18920473-18920492 None:intergenic 50.0%
TAGCTTCGATCCGGAGGTTT+GGG + chr1_2:18920506-18920525 None:intergenic 50.0%
ATAGCTTCGATCCGGAGGTT+TGG + chr1_2:18920507-18920526 None:intergenic 50.0%
TTCTGATAGCTTCGATCCGG+AGG + chr1_2:18920512-18920531 None:intergenic 50.0%
AAAAGCAGTCCAGGTTCAGC+AGG - chr1_2:18920722-18920741 Msa0060940:CDS 50.0%
CTACAAGAACCTGCTGAACC+TGG + chr1_2:18920734-18920753 None:intergenic 50.0%
GGTTCAGCAGGTTCTTGTAG+TGG - chr1_2:18920734-18920753 Msa0060940:CDS 50.0%
TGAAGAACTCCATGTTCCGG+AGG - chr1_2:18920979-18920998 Msa0060940:CDS 50.0%
GATTGTGCTGAACACGGTCA+TGG - chr1_2:18921138-18921157 Msa0060940:CDS 50.0%
TGAACACGGTCATGGCTGAA+AGG - chr1_2:18921146-18921165 Msa0060940:CDS 50.0%
TCGGTTCAGCTACCTTCAGT+AGG - chr1_2:18921188-18921207 Msa0060940:CDS 50.0%
CGGTTCAGCTACCTTCAGTA+GGG - chr1_2:18921189-18921208 Msa0060940:CDS 50.0%
!! ATCGAGAGCATCCCTACTGA+AGG + chr1_2:18921203-18921222 None:intergenic 50.0%
TTCAGTAGGGATGCTCTCGA+TGG - chr1_2:18921202-18921221 Msa0060940:CDS 50.0%
ACTTGTCGATAAGCACGACC+CGG - chr1_2:18921294-18921313 Msa0060940:intron 50.0%
GTATGTGGCGTTTATCTCCC+GGG + chr1_2:18921315-18921334 None:intergenic 50.0%
GTACAAGTCTCCCACTCACT+AGG + chr1_2:18921657-18921676 None:intergenic 50.0%
GTTCAAAGTCAGATGGGTGC+AGG - chr1_2:18921709-18921728 Msa0060940:CDS 50.0%
GGATGTGACAGAGGCTTACT+AGG - chr1_2:18921772-18921791 Msa0060940:CDS 50.0%
GCCACTTGTCAATGCAAGGT+AGG + chr1_2:18921948-18921967 None:intergenic 50.0%
GACAAGTGGCTCATCAAGCA+TGG - chr1_2:18921958-18921977 Msa0060940:CDS 50.0%
TTCACTCAATGGGATCCAGG+AGG - chr1_2:18922309-18922328 Msa0060940:CDS 50.0%
TCACTCAATGGGATCCAGGA+GGG - chr1_2:18922310-18922329 Msa0060940:CDS 50.0%
GAGGGTGGTCTCTTGTTCAT+TGG - chr1_2:18922328-18922347 Msa0060940:CDS 50.0%
GGTGGTCTCTTGTTCATTGG+AGG - chr1_2:18922331-18922350 Msa0060940:CDS 50.0%
TGGAAGAGACTGATGCAGGA+GGG - chr1_2:18922415-18922434 Msa0060940:CDS 50.0%
ATGACGAGGGCAGAGTTTGC+CGG - chr1_2:18919232-18919251 Msa0060940:CDS 55.0%
CATCACCGCGACTCTTACCA+CGG - chr1_2:18919255-18919274 Msa0060940:CDS 55.0%
GATGTCCGTGGTAAGAGTCG+CGG + chr1_2:18919263-18919282 None:intergenic 55.0%
GATGTCGGCGTGGACAACTA+AGG - chr1_2:18919300-18919319 Msa0060940:CDS 55.0%
! CGAAGCGTTTCCGACGTCTA+TGG - chr1_2:18919716-18919735 Msa0060940:CDS 55.0%
!! CAGATAGTGGAGCTAACGGG+AGG - chr1_2:18919823-18919842 Msa0060940:CDS 55.0%
!! GCGGTGTTGCCAAACCCTAA+AGG + chr1_2:18919882-18919901 None:intergenic 55.0%
TTAGGGTTTGGCAACACCGC+TGG - chr1_2:18919882-18919901 Msa0060940:CDS 55.0%
GTCCAGAACCATTCCACCAG+CGG + chr1_2:18919901-18919920 None:intergenic 55.0%
!!! TTTTCGGTGTCTGGTGGTGC+AGG + chr1_2:18920587-18920606 None:intergenic 55.0%
! GCTTCTTTCTCGGCACTGAG+GGG + chr1_2:18920671-18920690 None:intergenic 55.0%
! GGCTTCTTTCTCGGCACTGA+GGG + chr1_2:18920672-18920691 None:intergenic 55.0%
TGGCTTCTTTCTCGGCACTG+AGG + chr1_2:18920673-18920692 None:intergenic 55.0%
CTTGTCGATAAGCACGACCC+GGG - chr1_2:18921295-18921314 Msa0060940:intron 55.0%
TGAACAAGAGACCACCCTCC+TGG + chr1_2:18922327-18922346 None:intergenic 55.0%
CTCGAGGTCGAGTTCTTCAG+AGG - chr1_2:18922392-18922411 Msa0060940:CDS 55.0%
GAGGTCGAGTTCTTCAGAGG+TGG - chr1_2:18922395-18922414 Msa0060940:CDS 55.0%
GAGGTGGAAGAGACTGATGC+AGG - chr1_2:18922411-18922430 Msa0060940:CDS 55.0%
GTGGAAGAGACTGATGCAGG+AGG - chr1_2:18922414-18922433 Msa0060940:CDS 55.0%
GGTAAGAGTCGCGGTGATGC+CGG + chr1_2:18919254-18919273 None:intergenic 60.0%
TGGCAACACCGCTGGTGGAA+TGG - chr1_2:18919890-18919909 Msa0060940:CDS 60.0%
CACCGCTGGTGGAATGGTTC+TGG - chr1_2:18919896-18919915 Msa0060940:CDS 60.0%
! TTGGGTAGTGGCGGCAGAGT+CGG + chr1_2:18920488-18920507 None:intergenic 60.0%
! TCCGGAGGTTTGGGTAGTGG+CGG + chr1_2:18920497-18920516 None:intergenic 60.0%
CGATCCGGAGGTTTGGGTAG+TGG + chr1_2:18920500-18920519 None:intergenic 60.0%
! TGCAGGAAGTGGTGGAGGCA+AGG + chr1_2:18920570-18920589 None:intergenic 60.0%
! TCTGGTGGTGCAGGAAGTGG+TGG + chr1_2:18920578-18920597 None:intergenic 60.0%
! GTGTCTGGTGGTGCAGGAAG+TGG + chr1_2:18920581-18920600 None:intergenic 60.0%
TCTCCATCTGTGGCAGCCTC+CGG + chr1_2:18920998-18921017 None:intergenic 60.0%
CTCAATGGGATCCAGGAGGG+TGG - chr1_2:18922313-18922332 Msa0060940:CDS 60.0%
GGGTTTGGCAACACCGCTGG+TGG - chr1_2:18919885-18919904 Msa0060940:CDS 65.0%
GCCGCCACTACCCAAACCTC+CGG - chr1_2:18920493-18920512 Msa0060940:CDS 65.0%
GGTGGTGCAGGAAGTGGTGG+AGG + chr1_2:18920575-18920594 None:intergenic 65.0%
CTGTGGCAGCCTCCGGAACA+TGG + chr1_2:18920991-18921010 None:intergenic 65.0%
GTTCCGGAGGCTGCCACAGA+TGG - chr1_2:18920992-18921011 Msa0060940:CDS 65.0%
GGAGGCTGCCACAGATGGAG+AGG - chr1_2:18920997-18921016 Msa0060940:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 18919217 18922446 18919217 ID=Msa0060940;Name=Msa0060940
chr1_2 mRNA 18919217 18922446 18919217 ID=Msa0060940-mRNA-1;Parent=Msa0060940;Name=Msa0060940-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|3|0|955
chr1_2 exon 18922235 18922446 18922235 ID=Msa0060940-mRNA-1:exon:7326;Parent=Msa0060940-mRNA-1
chr1_2 exon 18921416 18921992 18921416 ID=Msa0060940-mRNA-1:exon:7325;Parent=Msa0060940-mRNA-1
chr1_2 exon 18919217 18921295 18919217 ID=Msa0060940-mRNA-1:exon:7324;Parent=Msa0060940-mRNA-1
chr1_2 CDS 18922235 18922446 18922235 ID=Msa0060940-mRNA-1:cds;Parent=Msa0060940-mRNA-1
chr1_2 CDS 18921416 18921992 18921416 ID=Msa0060940-mRNA-1:cds;Parent=Msa0060940-mRNA-1
chr1_2 CDS 18919217 18921295 18919217 ID=Msa0060940-mRNA-1:cds;Parent=Msa0060940-mRNA-1
Gene Sequence

>Msa0060940

ATGGGAGATCAACTGATGACGAGGGCAGAGTTTGCCGGCATCACCGCGACTCTTACCACGGACATCAAAGCTCTGTTAGATCAGATGTCGGCGTGGACAACTAAGGTTTACAACAACAACGCCAACAACAACAACAACAGAAACAATCCGAACAGAGGAGGAGAACCAATTCCGGTTATTAGGGTTCGTAATAACAACCATACTATTGTTACAATTCTTACGCAATTGAAAGAGGAGATCGACGTTGCTCGGACGACCGAAGCGTTTCCGACGTCTATGGAACCAGTTGAAGAACATTCAGTCATCGAACCTGTTGTTATTATTGAGGAGACTGATACTCAAACCGTGCTAAACAAAGAAATATCAGATAGTGGAGCTAACGGGAGGTGCCTAGATATTTCACTTCAAGCTCCACCTAGACCTTTAGGGTTTGGCAACACCGCTGGTGGAATGGTTCTGGACCACTCTCAAAGTTTCTCTAAAGGGGGATCATCACCAAAAGGCTTTTTGCAGGCTTTGAGTTTCAAAATAAAAGTGAATGTAGCAGATGGTGAAAGAACCAGCCTTCTTAAATCAGACCCTAAGACAATTGATTATGTTAAACAACAGAAAGAAAAATCAAAGGTCGAATGTTCCGAAAGTAATGATATCAGTGATGCAGTTGAACTTTCTATTGCAGCATCGGAGGCACTTGTCATACATGAGTTAGTAAAGATGGAGTCGGTATCAGAAACATTGATTGCAGAAGCTGTAATCAATGAACCAAAAACTAAAGATTCATCAGAAGATGTGGTCAATTTGACGAGCTGCAAAGGATCCTTGATTCATCTTTCTGGTATGTTAAACACATCATCTTTGAATTGTAATCCACTACTATCGAAACATTCACATCCACGTGTTTTCACTCCGACTCTGCCGCCACTACCCAAACCTCCGGATCGAAGCTATCAGAAGATTCTCGTGGATTTTCAAGTGACACAAAATTTCACCTTGCCTCCACCACTTCCTGCACCACCAGACACCGAAAATAGATTGGTGAAATTCATCACAACATCGGTTTCTTGCGCCCATTACATGTTTGTTGAAATACCCCTCAGTGCCGAGAAAGAAGCCATTGCGCATAATTCTGAAGTTCCATTTTGCAAAAGCAGTCCAGGTTCAGCAGGTTCTTGTAGTGGGGATCCTATAAACATTAGCTACCTTAATTTAAAGGAAATTAAGCTGAGTCGGAATGATTTTCATATGAAGGGTATTAATTCTTTTGATTTTGTTCATGTGCTTACCATGAAGCATAGTTCAGATTGCTACAAGGATCTCTATGACTTAACCATCGAAGTTGTTGAAACAGATCTACCGAATGGGATTGAAAGCAATGGAGTAGGTGATGACCGATATTTGAGTGAAGAACTCCATGTTCCGGAGGCTGCCACAGATGGAGAGGATAATAATACTAATGACGACCTCTTAAAATACAGTCAAGATGATGAATATGAAGTATTTGAGCTAAGAATTGTGCATGGAAAGAATAGGACTGGATTTGAAGAAAACAAGGAAGTGCTGATTGTGCTGAACACGGTCATGGCTGAAAGGTACTATGTGACAGATTATCTCGGTTCAGCTACCTTCAGTAGGGATGCTCTCGATGGTTGTTTGAAGATTATTAAAAATGACAAGGATTTCTTTCATCAAAGCCTAGATGAAATCAAGCTTCTTAAACTTGTCGATAAGCACGACCCGGGAGATAAACGCCACATACTGCGTCTTTATGACTATTTCTACCATCAGGAACATTTATTCATTGTGACCGAACTTCTGCGTGCAAACTTATATGAATTTCATAAATTCAACCAAGAATATGGTGGTGAAGCATATTTTACATTAAAGAGATTGCAGCTCATTACTCGACAGTGTTTAGAGGCATTGCAATATTTACATAACTTGGGAATTATTCAATGTGACCTGAAGCCTCATAATTCTGTCTTCTCTATTGAAGTATTTGTATGTTCTATTGATCTTTGGCATTCGAATAATTCTGGAATAGGATGTTTCTTATACTTGATTGATATTATTACTAGCCTAGTGAGTGGGAGACTTGTACGTGAAAGAGTTGGTGAAGCCATGTTGAATGCTGTTCAAAGTCAGATGGGTGCAGGTGAAATGAGATTTTATGAGTTTCAACATATCTTTGACATTGGATGTGACAGAGGCTTACTAGGAGCTTCAGTTGAAAAGATCCCAATAGTCTCAATCACAAGTGACAACAATTTTGATGCTTGTGGCGATATAATTTCCTGTTCAATTTGCCTTCAGGATTTCCAAATGGGAGAGACTGTTAGAAGCTTGCCCCATTATCATCATACGTTTAACCTACCTTGCATTGACAAGTGGCTCATCAAGCATGGCTTGTGCCCATTATGCAGAGACAGTGAGCAGCTAGTGCTTTCAAATAAGATTAATGTTTTTTGCAATGGCGTGAAAAGCATATATTTTCCAAGCCTACACTTAGTTGTGTGCAAGTGTGGTTTTTGTGGGACAGAAAAGCAAGCACTTAATGGATGGCAACAACACAAAGGTTCCAAATTAAGAGATTGGAAAACAAGTATTATTGTGAAAGACTCTAGGCTACCTCTAGAACAATGGATGGTGAAGGTTGCAAAATGTCATGCTACTATATTTCTTCAATGGGATCAAGTCAACAACTATATTTTTCAAGTCAAATCATCCTCGCATTTCACTCAATGGGATCCAGGAGGGTGGTCTCTTGTTCATTGGAGGAGTAAACATGCATGGGAAGCTCTTTATCAAAATGAGAACTCGAGGTCGAGTTCTTCAGAGGTGGAAGAGACTGATGCAGGAGGGTTTTATTAG
Protein sequence

>Msa0060940

MGDQLMTRAEFAGITATLTTDIKALLDQMSAWTTKVYNNNANNNNNRNNPNRGGEPIPVIRVRNNNHTIVTILTQLKEEIDVARTTEAFPTSMEPVEEHSVIEPVVIIEETDTQTVLNKEISDSGANGRCLDISLQAPPRPLGFGNTAGGMVLDHSQSFSKGGSSPKGFLQALSFKIKVNVADGERTSLLKSDPKTIDYVKQQKEKSKVECSESNDISDAVELSIAASEALVIHELVKMESVSETLIAEAVINEPKTKDSSEDVVNLTSCKGSLIHLSGMLNTSSLNCNPLLSKHSHPRVFTPTLPPLPKPPDRSYQKILVDFQVTQNFTLPPPLPAPPDTENRLVKFITTSVSCAHYMFVEIPLSAEKEAIAHNSEVPFCKSSPGSAGSCSGDPINISYLNLKEIKLSRNDFHMKGINSFDFVHVLTMKHSSDCYKDLYDLTIEVVETDLPNGIESNGVGDDRYLSEELHVPEAATDGEDNNTNDDLLKYSQDDEYEVFELRIVHGKNRTGFEENKEVLIVLNTVMAERYYVTDYLGSATFSRDALDGCLKIIKNDKDFFHQSLDEIKLLKLVDKHDPGDKRHILRLYDYFYHQEHLFIVTELLRANLYEFHKFNQEYGGEAYFTLKRLQLITRQCLEALQYLHNLGIIQCDLKPHNSVFSIEVFVCSIDLWHSNNSGIGCFLYLIDIITSLVSGRLVRERVGEAMLNAVQSQMGAGEMRFYEFQHIFDIGCDRGLLGASVEKIPIVSITSDNNFDACGDIISCSICLQDFQMGETVRSLPHYHHTFNLPCIDKWLIKHGLCPLCRDSEQLVLSNKINVFCNGVKSIYFPSLHLVVCKCGFCGTEKQALNGWQQHKGSKLRDWKTSIIVKDSRLPLEQWMVKVAKCHATIFLQWDQVNNYIFQVKSSSHFTQWDPGGWSLVHWRSKHAWEALYQNENSRSSSSEVEETDAGGFY*