AlfalfaGEDB | Msa0062510

M. sativa cultivar zhongmu-4 / Msa0062510

KEGG: K02937
GO: GO:0000463 GO:0000470 GO:0001701 GO:0001824 GO:0001825 GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009792 GO:0009987 GO:0010467 GO:0015934 GO:0016070 GO:0016072 GO:0019538 GO:0022613 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042273 GO:0043009 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0048646 GO:0048856 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0062510	XP_013466755.2	73.984	615	98	7	1	615	92	644	0.0	868

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0062510	sp\|Q8L3W1\|VRN1_ARATH	28.367	349	195	10	18	326	6	339	4.23e-35	139
Msa0062510	sp\|Q8L3W1\|VRN1_ARATH	34.906	106	64	3	376	479	230	332	3.28e-11	68.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0062510	A0A072VGT1	91.479	399	30	1	33	431	1	395	0.0	725

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TFs/TRs:

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Gene ID	Type	Classification
Msa0062510	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0062510	MtrunA17_Chr1g0163071	91.391	302	22	1	33	334	1	298	0.0	542
Msa0062510	MtrunA17_Chr1g0208771	46.725	458	216	11	34	479	13	454	1.09e-124	376
Msa0062510	MtrunA17_Chr1g0208771	35.772	123	76	2	476	595	9	131	1.18e-11	67.4
Msa0062510	MtrunA17_Chr8g0390841	41.359	515	238	15	1	478	26	513	8.96e-116	355
Msa0062510	MtrunA17_Chr1g0163081	83.673	147	23	1	475	621	1	146	1.25e-85	263
Msa0062510	MtrunA17_Chr1g0162161	75.839	149	32	1	477	621	1	149	1.67e-75	238
Msa0062510	MtrunA17_Chr1g0163101	75.862	145	33	2	479	621	32	176	5.02e-72	229
Msa0062510	MtrunA17_Chr1g0163101	33.333	144	88	4	13	151	12	152	4.16e-15	73.9
Msa0062510	MtrunA17_Chr1g0163121	72.414	145	33	1	477	621	8	145	2.61e-67	216
Msa0062510	MtrunA17_Chr1g0163121	33.058	121	74	4	32	148	9	126	1.61e-11	62.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0062510	AT3G18990.1	28.367	349	195	10	18	326	6	339	4.30e-36	139
Msa0062510	AT3G18990.1	34.906	106	64	3	376	479	230	332	3.33e-12	68.6
Msa0062510	AT3G18990.2	27.954	347	199	9	18	326	6	339	1.96e-35	137
Msa0062510	AT3G18990.2	34.906	106	64	3	376	479	230	332	4.75e-12	68.6
Msa0062510	AT4G01580.1	32.857	140	85	2	17	154	29	161	6.31e-18	82.8
Msa0062510	AT3G18960.2	36.275	102	65	0	17	118	29	130	9.38e-18	82.4
Msa0062510	AT3G18960.1	36.275	102	65	0	17	118	29	130	1.34e-17	82.4
Msa0062510	AT4G33280.1	24.837	306	201	10	31	326	36	322	2.70e-15	78.2
Msa0062510	AT1G49480.3	31.081	148	94	5	186	326	78	224	3.72e-15	75.5
Msa0062510	AT1G49480.3	34.906	106	64	3	376	479	115	217	5.64e-13	69.3

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Find 101 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTGGCCTGATTTCATGT+TGG	0.576131	1_2:-22060515	None:intergenic
AAAGATTTGCACGATCGCTT+AGG	0.496687	1_2:+22062044	Msa0062510:CDS
AACAACAATGACTAGCAATA+AGG	0.315318	1_2:+22057925	None:intergenic
AACAAGTTGGATTACCTTGA+AGG	0.449668	1_2:-22059771	None:intergenic
AACCTGATTTGGAAGTTTAA+GGG	0.367656	1_2:-22061966	None:intergenic
AACGAATAGTAGAAGCAGAG+AGG	0.651419	1_2:-22057961	None:intergenic
AACTCTTGGATGGGAGAGTT+TGG	0.242668	1_2:+22059584	Msa0062510:CDS
AAGCATCATGTCCAATCTAG+TGG	0.644078	1_2:+22059028	Msa0062510:CDS
AATATTATGCCAAATCAACG+AGG	0.719522	1_2:+22060260	Msa0062510:CDS
AATCAAATAATCCTTCTTTC+AGG	0.198997	1_2:+22059260	Msa0062510:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAGACAAAAATTAACCCTT+TGG	+	chr1_2:22058208-22058227	Msa0062510:intron	30.0%
AATCTATTCCCATTTCCAAA+GGG	-	chr1_2:22058226-22058245	None:intergenic	30.0%
TAATCTATTCCCATTTCCAA+AGG	-	chr1_2:22058227-22058246	None:intergenic	30.0%
GATTTGACATGTCATTCATA+TGG	-	chr1_2:22058601-22058620	None:intergenic	30.0%
ACACATTATTCACTAGATCA+TGG	+	chr1_2:22058719-22058738	Msa0062510:CDS	30.0%
CACATTATTCACTAGATCAT+GGG	+	chr1_2:22058720-22058739	Msa0062510:CDS	30.0%
GAAAGGATATTCAATCTCAA+GGG	-	chr1_2:22058810-22058829	None:intergenic	30.0%
CAAGAATTATACCACTAGAT+TGG	-	chr1_2:22059042-22059061	None:intergenic	30.0%
TTCGACTTCAAAATTTCCAT+TGG	-	chr1_2:22059095-22059114	None:intergenic	30.0%
AATTAATCAAAGACTCACCA+AGG	-	chr1_2:22059320-22059339	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	22057932	22062342	22057932	ID=Msa0062510;Name=Msa0062510
chr1_2	mRNA	22057932	22062342	22057932	ID=Msa0062510-mRNA-1;Parent=Msa0062510;Name=Msa0062510-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|0\|0\|0.87\|1\|1\|8\|0\|621
chr1_2	exon	22057932	22058027	22057932	ID=Msa0062510-mRNA-1:exon:8134;Parent=Msa0062510-mRNA-1
chr1_2	exon	22058572	22059049	22058572	ID=Msa0062510-mRNA-1:exon:8135;Parent=Msa0062510-mRNA-1
chr1_2	exon	22059149	22059321	22059149	ID=Msa0062510-mRNA-1:exon:8136;Parent=Msa0062510-mRNA-1
chr1_2	exon	22059523	22059778	22059523	ID=Msa0062510-mRNA-1:exon:8137;Parent=Msa0062510-mRNA-1
chr1_2	exon	22059868	22059930	22059868	ID=Msa0062510-mRNA-1:exon:8138;Parent=Msa0062510-mRNA-1
chr1_2	exon	22060150	22060295	22060150	ID=Msa0062510-mRNA-1:exon:8139;Parent=Msa0062510-mRNA-1
chr1_2	exon	22060412	22060633	22060412	ID=Msa0062510-mRNA-1:exon:8140;Parent=Msa0062510-mRNA-1
chr1_2	exon	22061911	22062342	22061911	ID=Msa0062510-mRNA-1:exon:8141;Parent=Msa0062510-mRNA-1
chr1_2	CDS	22057932	22058027	22057932	ID=Msa0062510-mRNA-1:cds;Parent=Msa0062510-mRNA-1
chr1_2	CDS	22058572	22059049	22058572	ID=Msa0062510-mRNA-1:cds;Parent=Msa0062510-mRNA-1
chr1_2	CDS	22059149	22059321	22059149	ID=Msa0062510-mRNA-1:cds;Parent=Msa0062510-mRNA-1
chr1_2	CDS	22059523	22059778	22059523	ID=Msa0062510-mRNA-1:cds;Parent=Msa0062510-mRNA-1
chr1_2	CDS	22059868	22059930	22059868	ID=Msa0062510-mRNA-1:cds;Parent=Msa0062510-mRNA-1
chr1_2	CDS	22060150	22060295	22060150	ID=Msa0062510-mRNA-1:cds;Parent=Msa0062510-mRNA-1
chr1_2	CDS	22060412	22060633	22060412	ID=Msa0062510-mRNA-1:cds;Parent=Msa0062510-mRNA-1
chr1_2	CDS	22061911	22062342	22061911	ID=Msa0062510-mRNA-1:cds;Parent=Msa0062510-mRNA-1

Gene Sequence

>Msa0062510

ATGACTAGCAATAAGGGAACTAAGCATACCCTCTCTGCTTCTACTATTCGTTTCTTTAGAGTTATTACCACACCAATTCTTCAAGATGGAAAGCTTATGATACCAAAAGGTTTCACTAAGGACCATATGAATGACATGTCAAATCCAATGTTTCTCAAGACTCCAGATGGCAAAAATTGGGAAATGAGTGCGACTAAAATCAATGGGGATTTTTGGTTTCAAAAGGGTTGGAACGAATTTGCTACACATTATTCACTAGATCATGGGCATATGGTATTATTTCAATACGAAGAAAACTCTCATTTTGTGGTTCATATATTTTCCAAGAGTACCCTTGAGATTGAATATCCTTTCCATGAAAATCAACATGAACAAAGCAACTTTGATCAACAAAGTAGTGATGAGGACTCCATTGAAATTTTGGACAAGTCACCTTCAAGCAGGAAGAAAACTAGACCAAAATCACCAATTATGTGTCCTCAACCACATAAGAAATTGAGAAATGACTCAAGTGAAGGTGTTGGAACAAGCTCGAGATTCCAAAACTTGCCTAAGCATCATGTCCAATCTAGTGAGGATACGGGTGCTAGCGCTGAATTCCGAAAAGTAAAGCACGAGCTAGAACAAGATCAAGACAAAAAGCAATACCAAGAGCAGGAGCAGTTAAATAGAGTTAGAAGCTTCAAATCAAATAATCCTTCTTTCAGGATTACCATGAAGTCTTCACATATTTATAGTCATTCCTTGTATGTCCCATCACAATTTGCTAACAATCACATGAAGAAAGAGCAAAGTGACATTCTTCTTCAACTCTTGGATGGGAGAGTTTGGGATGCTAAATATTTTTCTGGAAGAATTAAAGGTGGATGGAAGAAATTTGCAGTACACAACAAGTTGAAAATTGGGGATGTGTGTATTTTTGAACTAACCAAAAGCCAATACCTTACTTTGAAAGTAATAATATTTAGACTTGAGGAAGATCCACATTCCCCTCCCCTTCAAGAATCAGTTCAACGAGACAAAGACTTCTCGGATGTTGAAAGTGAAACTATTATGACAAATAAAGGAGAGAAGGAAACCGAAAAAACTTCATTGCGGACAAAGGCTGAATTCTTTACTAATGAAGCTGAGAACCAAGCTGATAAATTCAACTCCAAATATCCATTTTTCAAGGTCAATATTATGCCAAATCAACGAGGGCATTCGAGATTGCATGTACCAAATTCCTTCATTACAAATTACTTGGATAACAAGGAACAGATGGTGATGTTAAAGTTTGGGAAGAAATTGTGGCCTGTAAAGTTGCATTGTTATTCCAACATGAAATCAGGCCAGTTTTGTAGAGGTTGGAAGCTATTTTGGGAAGAAAATAAATTGAAAGGTGGAGATGCATGTGTTTTTGAGCTCATCAAGAGGGAAGATGTTGTGCTTAATATGATTCCAAGGATTTTTGTAGAAAGATATTGGAAAGATGTATCAAATCCAATATCCCTTAAACTTCCAAATCAGGTTGAGTGCAAAATGTTTTGGGTACAACGCGGTTATGATATTTGGCTAGTGAATTGGAAAAGATTTGCACGATCGCTTAGGTGTGGAGATCTTTTAGTTTTTCAATATAAAGGAGGGTCAGATTTTCATGTCATTGTATTAGATGATAGTAAATTGGAAATTGACTATTCAAGTATGAGATGCAACGACGAACAAGATTCTAACAAGCACTTTAAACAAGAAGAAGAAAGTGATTATGATTGTGTTGAGATTCTGAGTGATATTTCTACACCTCAAGGGACAAACATTGACAAGAAAAAAATTATTATGTCTTCAACCCAGCAAAAAATCACAGGCAAGTTCATCTACTTGATTTGA

Protein sequence

>Msa0062510

MTSNKGTKHTLSASTIRFFRVITTPILQDGKLMIPKGFTKDHMNDMSNPMFLKTPDGKNWEMSATKINGDFWFQKGWNEFATHYSLDHGHMVLFQYEENSHFVVHIFSKSTLEIEYPFHENQHEQSNFDQQSSDEDSIEILDKSPSSRKKTRPKSPIMCPQPHKKLRNDSSEGVGTSSRFQNLPKHHVQSSEDTGASAEFRKVKHELEQDQDKKQYQEQEQLNRVRSFKSNNPSFRITMKSSHIYSHSLYVPSQFANNHMKKEQSDILLQLLDGRVWDAKYFSGRIKGGWKKFAVHNKLKIGDVCIFELTKSQYLTLKVIIFRLEEDPHSPPLQESVQRDKDFSDVESETIMTNKGEKETEKTSLRTKAEFFTNEAENQADKFNSKYPFFKVNIMPNQRGHSRLHVPNSFITNYLDNKEQMVMLKFGKKLWPVKLHCYSNMKSGQFCRGWKLFWEENKLKGGDACVFELIKREDVVLNMIPRIFVERYWKDVSNPISLKLPNQVECKMFWVQRGYDIWLVNWKRFARSLRCGDLLVFQYKGGSDFHVIVLDDSKLEIDYSSMRCNDEQDSNKHFKQEEESDYDCVEILSDISTPQGTNIDKKKIIMSSTQQKITGKFIYLI*