Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0065210 | G7I6L4 | 87.313 | 134 | 17 | 0 | 1 | 134 | 1 | 134 | 3.38e-74 | 226 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0065210 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0065210 | MtrunA17_Chr5g0399781 | 51.852 | 54 | 25 | 1 | 3 | 56 | 12 | 64 | 1.43e-11 | 59.7 |
| Msa0065210 | MtrunA17_Chr4g0055611 | 66.667 | 36 | 12 | 0 | 3 | 38 | 12 | 47 | 7.05e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1_2:+27113548 | Msa0065210:CDS |
| CCACAAGCATTACATAGTAT+TGG | 0.433061 | 1_2:-27113097 | None:intergenic |
| TGGAGATTGGTTTGAAAGCT+CGG | 0.447768 | 1_2:-27113224 | None:intergenic |
| GAAACTAAACTTTGGCGTAA+AGG | 0.456777 | 1_2:+27113061 | Msa0065210:CDS |
| TGCTTGTGGACTACATTTCA+AGG | 0.461822 | 1_2:+27113111 | Msa0065210:CDS |
| ATCAGACCTTGACGTTGAAA+AGG | 0.465983 | 1_2:+27113186 | Msa0065210:CDS |
| GGACTACATTTCAAGGCTAA+AGG | 0.474721 | 1_2:+27113118 | Msa0065210:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1_2:-27113144 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.478624 | 1_2:+27113637 | Msa0065210:CDS |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1_2:+27113516 | Msa0065210:CDS |
| CATAGTATTGGCTTCTCATA+CGG | 0.492997 | 1_2:-27113085 | None:intergenic |
| GGAGATTGGTTTGAAAGCTC+GGG | 0.493147 | 1_2:-27113223 | None:intergenic |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1_2:+27113643 | Msa0065210:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1_2:-27113514 | None:intergenic |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1_2:+27113549 | Msa0065210:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1_2:-27113643 | None:intergenic |
| GAGCTTTCAAACCAATCTCC+AGG | 0.568278 | 1_2:+27113226 | Msa0065210:CDS |
| CCAATACTATGTAATGCTTG+TGG | 0.579274 | 1_2:+27113097 | Msa0065210:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1_2:-27113143 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1_2:+27113519 | Msa0065210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAATTTTTTTTGTAGAA+AGG | + | chr1_2:27113488-27113507 | Msa0065210:intron | 10.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_2:27113286-27113305 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_2:27113287-27113306 | None:intergenic | 15.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_2:27113247-27113266 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_2:27113288-27113307 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_2:27113341-27113360 | Msa0065210:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_2:27113466-27113485 | None:intergenic | 20.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_2:27113053-27113072 | Msa0065210:CDS | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_2:27113240-27113259 | None:intergenic | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_2:27113374-27113393 | None:intergenic | 25.0% |
| ACTCCACATTCATACATTTA+TGG | + | chr1_2:27113368-27113387 | Msa0065210:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_2:27113517-27113536 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_2:27113516-27113535 | Msa0065210:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_2:27113548-27113567 | Msa0065210:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_2:27113549-27113568 | Msa0065210:CDS | 30.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_2:27113061-27113080 | Msa0065210:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_2:27113088-27113107 | None:intergenic | 35.0% |
| CCACAAGCATTACATAGTAT+TGG | - | chr1_2:27113100-27113119 | None:intergenic | 35.0% | |
| CCAATACTATGTAATGCTTG+TGG | + | chr1_2:27113097-27113116 | Msa0065210:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_2:27113146-27113165 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_2:27113147-27113166 | None:intergenic | 35.0% | |
| !! | GTCTGATAACTGATTTTGCT+GGG | - | chr1_2:27113173-27113192 | None:intergenic | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_2:27113453-27113472 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_2:27113519-27113538 | Msa0065210:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_2:27113637-27113656 | Msa0065210:CDS | 35.0% | |
| ! | TGCTTGTGGACTACATTTCA+AGG | + | chr1_2:27113111-27113130 | Msa0065210:CDS | 40.0% |
| GGACTACATTTCAAGGCTAA+AGG | + | chr1_2:27113118-27113137 | Msa0065210:CDS | 40.0% | |
| ! | TGATTTTGCTGGGATGTCTT+CGG | - | chr1_2:27113163-27113182 | None:intergenic | 40.0% |
| !! | GGTCTGATAACTGATTTTGC+TGG | - | chr1_2:27113174-27113193 | None:intergenic | 40.0% |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_2:27113186-27113205 | Msa0065210:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_2:27113195-27113214 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_2:27113227-27113246 | None:intergenic | 40.0% |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_2:27113226-27113245 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_2:27113226-27113245 | Msa0065210:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_2:27113646-27113665 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_2:27113643-27113662 | Msa0065210:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 27113049 | 27113711 | 27113049 | ID=Msa0065210;Name=Msa0065210 |
| chr1_2 | mRNA | 27113049 | 27113711 | 27113049 | ID=Msa0065210-mRNA-1;Parent=Msa0065210;Name=Msa0065210-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|134 |
| chr1_2 | exon | 27113049 | 27113247 | 27113049 | ID=Msa0065210-mRNA-1:exon:9504;Parent=Msa0065210-mRNA-1 |
| chr1_2 | exon | 27113506 | 27113711 | 27113506 | ID=Msa0065210-mRNA-1:exon:9505;Parent=Msa0065210-mRNA-1 |
| chr1_2 | CDS | 27113049 | 27113247 | 27113049 | ID=Msa0065210-mRNA-1:cds;Parent=Msa0065210-mRNA-1 |
| chr1_2 | CDS | 27113506 | 27113711 | 27113506 | ID=Msa0065210-mRNA-1:cds;Parent=Msa0065210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
