Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0065220 | AES60398.2 | 86.567 | 134 | 18 | 0 | 1 | 134 | 1 | 134 | 8.18e-73 | 223 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0065220 | G7I6L4 | 86.567 | 134 | 18 | 0 | 1 | 134 | 1 | 134 | 3.91e-73 | 223 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0065220 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0065220 | MtrunA17_Chr5g0399781 | 51.852 | 54 | 25 | 1 | 3 | 56 | 12 | 64 | 1.26e-11 | 60.1 |
| Msa0065220 | MtrunA17_Chr4g0055611 | 66.667 | 36 | 12 | 0 | 3 | 38 | 12 | 47 | 6.34e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1_2:+27127380 | Msa0065220:CDS |
| GAGCTTTCAAACCAATTTCC+AGG | 0.378813 | 1_2:+27126900 | Msa0065220:CDS |
| CGCTTGTGGACTACATTTCA+AGG | 0.405648 | 1_2:+27126785 | Msa0065220:CDS |
| TGGAAATTGGTTTGAAAGCT+CGG | 0.417197 | 1_2:-27126898 | None:intergenic |
| CCACAAGCGTTACATAGTAT+TGG | 0.448818 | 1_2:-27126771 | None:intergenic |
| GAAACTAAACTTTGGCGTAA+AGG | 0.456777 | 1_2:+27126735 | Msa0065220:CDS |
| GGACTACATTTCAAGGCTAA+AGG | 0.474721 | 1_2:+27126792 | Msa0065220:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1_2:-27126818 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.478624 | 1_2:+27127469 | Msa0065220:CDS |
| ATCAGACCTTGACATTGAAA+AGG | 0.486095 | 1_2:+27126860 | Msa0065220:CDS |
| GGAAATTGGTTTGAAAGCTC+GGG | 0.487200 | 1_2:-27126897 | None:intergenic |
| CATAGTATTGGCTTCTCATA+CGG | 0.490978 | 1_2:-27126759 | None:intergenic |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1_2:+27127348 | Msa0065220:CDS |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1_2:+27127475 | Msa0065220:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1_2:-27127346 | None:intergenic |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1_2:+27127381 | Msa0065220:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1_2:-27127475 | None:intergenic |
| CCAATACTATGTAACGCTTG+TGG | 0.608782 | 1_2:+27126771 | Msa0065220:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1_2:-27126817 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1_2:+27127351 | Msa0065220:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_2:27126960-27126979 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_2:27126961-27126980 | None:intergenic | 15.0% |
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1_2:27127320-27127339 | Msa0065220:intron | 15.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_2:27126960-27126979 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_2:27126961-27126980 | None:intergenic | 15.0% |
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1_2:27127320-27127339 | Msa0065220:intron | 15.0% |
| !!! | ATTTTTTATTACCTGGAAAT+TGG | - | chr1_2:27126914-27126933 | None:intergenic | 20.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_2:27126921-27126940 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_2:27126962-27126981 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_2:27127015-27127034 | Msa0065220:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_2:27127066-27127085 | Msa0065220:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_2:27127159-27127178 | Msa0065220:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_2:27127160-27127179 | Msa0065220:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_2:27127300-27127319 | None:intergenic | 20.0% |
| !!! | ATTTTTTATTACCTGGAAAT+TGG | - | chr1_2:27126914-27126933 | None:intergenic | 20.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_2:27126921-27126940 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_2:27126962-27126981 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_2:27127015-27127034 | Msa0065220:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_2:27127066-27127085 | Msa0065220:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_2:27127159-27127178 | Msa0065220:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_2:27127160-27127179 | Msa0065220:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_2:27127300-27127319 | None:intergenic | 20.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_2:27126727-27126746 | Msa0065220:CDS | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_2:27127026-27127045 | Msa0065220:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_2:27127106-27127125 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_2:27127107-27127126 | None:intergenic | 25.0% |
| ! | ATTCATTCAAAACCTAATAC+CGG | - | chr1_2:27127151-27127170 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_2:27127161-27127180 | Msa0065220:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_2:27127209-27127228 | None:intergenic | 25.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_2:27126727-27126746 | Msa0065220:CDS | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_2:27127026-27127045 | Msa0065220:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_2:27127106-27127125 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_2:27127107-27127126 | None:intergenic | 25.0% |
| ! | ATTCATTCAAAACCTAATAC+CGG | - | chr1_2:27127151-27127170 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_2:27127161-27127180 | Msa0065220:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_2:27127209-27127228 | None:intergenic | 25.0% |
| !! | GTCTGATAACTGATTTTGTT+GGG | - | chr1_2:27126847-27126866 | None:intergenic | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_2:27127114-27127133 | None:intergenic | 30.0% | |
| TTCATTCAAAACCTAATACC+GGG | - | chr1_2:27127150-27127169 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_2:27127203-27127222 | Msa0065220:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_2:27127349-27127368 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_2:27127348-27127367 | Msa0065220:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_2:27127380-27127399 | Msa0065220:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_2:27127381-27127400 | Msa0065220:CDS | 30.0% | |
| !! | GTCTGATAACTGATTTTGTT+GGG | - | chr1_2:27126847-27126866 | None:intergenic | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_2:27127114-27127133 | None:intergenic | 30.0% | |
| TTCATTCAAAACCTAATACC+GGG | - | chr1_2:27127150-27127169 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_2:27127203-27127222 | Msa0065220:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_2:27127349-27127368 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_2:27127348-27127367 | Msa0065220:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_2:27127380-27127399 | Msa0065220:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_2:27127381-27127400 | Msa0065220:CDS | 30.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_2:27126735-27126754 | Msa0065220:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_2:27126762-27126781 | None:intergenic | 35.0% |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_2:27126820-27126839 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_2:27126821-27126840 | None:intergenic | 35.0% | |
| ! | TGATTTTGTTGGGATGTCTT+CGG | - | chr1_2:27126837-27126856 | None:intergenic | 35.0% |
| !! | GGTCTGATAACTGATTTTGT+TGG | - | chr1_2:27126848-27126867 | None:intergenic | 35.0% |
| ATCAGACCTTGACATTGAAA+AGG | + | chr1_2:27126860-27126879 | Msa0065220:CDS | 35.0% | |
| ! | CAGATTCCTTTTCAATGTCA+AGG | - | chr1_2:27126869-27126888 | None:intergenic | 35.0% |
| !! | TGGAAATTGGTTTGAAAGCT+CGG | - | chr1_2:27126901-27126920 | None:intergenic | 35.0% |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_2:27127167-27127186 | Msa0065220:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_2:27127287-27127306 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_2:27127351-27127370 | Msa0065220:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_2:27127469-27127488 | Msa0065220:CDS | 35.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_2:27126735-27126754 | Msa0065220:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_2:27126762-27126781 | None:intergenic | 35.0% |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_2:27126820-27126839 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_2:27126821-27126840 | None:intergenic | 35.0% | |
| ! | TGATTTTGTTGGGATGTCTT+CGG | - | chr1_2:27126837-27126856 | None:intergenic | 35.0% |
| !! | GGTCTGATAACTGATTTTGT+TGG | - | chr1_2:27126848-27126867 | None:intergenic | 35.0% |
| ATCAGACCTTGACATTGAAA+AGG | + | chr1_2:27126860-27126879 | Msa0065220:CDS | 35.0% | |
| ! | CAGATTCCTTTTCAATGTCA+AGG | - | chr1_2:27126869-27126888 | None:intergenic | 35.0% |
| !! | TGGAAATTGGTTTGAAAGCT+CGG | - | chr1_2:27126901-27126920 | None:intergenic | 35.0% |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_2:27127167-27127186 | Msa0065220:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_2:27127287-27127306 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_2:27127351-27127370 | Msa0065220:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_2:27127469-27127488 | Msa0065220:CDS | 35.0% | |
| CCACAAGCGTTACATAGTAT+TGG | - | chr1_2:27126774-27126793 | None:intergenic | 40.0% | |
| CCAATACTATGTAACGCTTG+TGG | + | chr1_2:27126771-27126790 | Msa0065220:CDS | 40.0% | |
| GGACTACATTTCAAGGCTAA+AGG | + | chr1_2:27126792-27126811 | Msa0065220:CDS | 40.0% | |
| !! | GGAAATTGGTTTGAAAGCTC+GGG | - | chr1_2:27126900-27126919 | None:intergenic | 40.0% |
| GAGCTTTCAAACCAATTTCC+AGG | + | chr1_2:27126900-27126919 | Msa0065220:CDS | 40.0% | |
| AGCATTCATTTGAGTAGACC+CGG | + | chr1_2:27127129-27127148 | Msa0065220:intron | 40.0% | |
| ATTTGAGTAGACCCGGTATT+AGG | + | chr1_2:27127136-27127155 | Msa0065220:intron | 40.0% | |
| CCACAAGCGTTACATAGTAT+TGG | - | chr1_2:27126774-27126793 | None:intergenic | 40.0% | |
| CCAATACTATGTAACGCTTG+TGG | + | chr1_2:27126771-27126790 | Msa0065220:CDS | 40.0% | |
| GGACTACATTTCAAGGCTAA+AGG | + | chr1_2:27126792-27126811 | Msa0065220:CDS | 40.0% | |
| !! | GGAAATTGGTTTGAAAGCTC+GGG | - | chr1_2:27126900-27126919 | None:intergenic | 40.0% |
| GAGCTTTCAAACCAATTTCC+AGG | + | chr1_2:27126900-27126919 | Msa0065220:CDS | 40.0% | |
| AGCATTCATTTGAGTAGACC+CGG | + | chr1_2:27127129-27127148 | Msa0065220:intron | 40.0% | |
| ATTTGAGTAGACCCGGTATT+AGG | + | chr1_2:27127136-27127155 | Msa0065220:intron | 40.0% | |
| ! | CGCTTGTGGACTACATTTCA+AGG | + | chr1_2:27126785-27126804 | Msa0065220:CDS | 45.0% |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_2:27127478-27127497 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_2:27127475-27127494 | Msa0065220:CDS | 45.0% | |
| ! | CGCTTGTGGACTACATTTCA+AGG | + | chr1_2:27126785-27126804 | Msa0065220:CDS | 45.0% |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_2:27127478-27127497 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_2:27127475-27127494 | Msa0065220:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 27126723 | 27127543 | 27126723 | ID=Msa0065220;Name=Msa0065220 |
| chr1_2 | mRNA | 27126723 | 27127543 | 27126723 | ID=Msa0065220-mRNA-1;Parent=Msa0065220;Name=Msa0065220-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|134 |
| chr1_2 | exon | 27126723 | 27126921 | 27126723 | ID=Msa0065220-mRNA-1:exon:9502;Parent=Msa0065220-mRNA-1 |
| chr1_2 | exon | 27127338 | 27127543 | 27127338 | ID=Msa0065220-mRNA-1:exon:9503;Parent=Msa0065220-mRNA-1 |
| chr1_2 | CDS | 27126723 | 27126921 | 27126723 | ID=Msa0065220-mRNA-1:cds;Parent=Msa0065220-mRNA-1 |
| chr1_2 | CDS | 27127338 | 27127543 | 27127338 | ID=Msa0065220-mRNA-1:cds;Parent=Msa0065220-mRNA-1 |
| Gene Sequence |
| Protein sequence |
