AlfalfaGEDB | Msa0065230

M. sativa cultivar zhongmu-4 / Msa0065230

KEGG: -
GO: -
PFAM: PF00320.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0065230	G7I6L4	86.567	134	18	0	1	134	1	134	9.81e-74	225

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TFs/TRs:

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Gene ID	Type	Classification
Msa0065230	TF	C2C2-GATA

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0065230	MtrunA17_Chr5g0399781	52.830	53	24	1	3	55	12	63	4.50e-11	58.5
Msa0065230	MtrunA17_Chr4g0055611	66.667	36	12	0	3	38	12	47	6.46e-11	58.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0065230	AT5G47140.1	38.889	72	40	1	3	70	12	83	4.13e-11	59.7

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Find 20 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAAAGCTTCAAAGTCTA+TGG	0.184389	1_2:+27156297	Msa0065230:CDS
ACAAAAGCTTCAAAGTCTAT+GGG	0.556384	1_2:+27156298	Msa0065230:CDS
ATCAGACCATGACGTTGAAA+AGG	0.486360	1_2:+27155777	Msa0065230:CDS
ATCGATTGTGAAGAATCAAA+TGG	0.491491	1_2:+27156265	Msa0065230:CDS
ATTTGATTCTTCACAATCGA+TGG	0.523804	1_2:-27156263	None:intergenic
CATAGTATTGGCTTCTCATA+CGG	0.492997	1_2:-27155676	None:intergenic
CCAAGCAATGAGATAGGCAT+TGG	0.496933	1_2:+27156392	Msa0065230:CDS
CCAATACTATGTAATGCTTG+TGG	0.579274	1_2:+27155688	Msa0065230:CDS
CCAATGCCTATCTCATTGCT+TGG	0.566560	1_2:-27156392	None:intergenic
CCACAAGCATTACATAGTAT+TGG	0.433061	1_2:-27155688	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGCTCTTATTGACAATGAA+AGG	-	chr1_2:27156031-27156050	None:intergenic	30.0%
ACTCCACATTCATACATTTA+TGG	+	chr1_2:27156120-27156139	Msa0065230:intron	30.0%
ATTTGATTCTTCACAATCGA+TGG	-	chr1_2:27156266-27156285	None:intergenic	30.0%
ATCGATTGTGAAGAATCAAA+TGG	+	chr1_2:27156265-27156284	Msa0065230:CDS	30.0%
AACAAAAGCTTCAAAGTCTA+TGG	+	chr1_2:27156297-27156316	Msa0065230:CDS	30.0%
ACAAAAGCTTCAAAGTCTAT+GGG	+	chr1_2:27156298-27156317	Msa0065230:CDS	30.0%
ATGCTCTTATTGACAATGAA+AGG	-	chr1_2:27156031-27156050	None:intergenic	30.0%
ACTCCACATTCATACATTTA+TGG	+	chr1_2:27156120-27156139	Msa0065230:intron	30.0%
ATTTGATTCTTCACAATCGA+TGG	-	chr1_2:27156266-27156285	None:intergenic	30.0%
ATCGATTGTGAAGAATCAAA+TGG	+	chr1_2:27156265-27156284	Msa0065230:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	27155640	27156460	27155640	ID=Msa0065230;Name=Msa0065230
chr1_2	mRNA	27155640	27156460	27155640	ID=Msa0065230-mRNA-1;Parent=Msa0065230;Name=Msa0065230-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0\|0\|0\|1\|1\|1\|2\|0\|134
chr1_2	exon	27155640	27155838	27155640	ID=Msa0065230-mRNA-1:exon:9506;Parent=Msa0065230-mRNA-1
chr1_2	exon	27156255	27156460	27156255	ID=Msa0065230-mRNA-1:exon:9507;Parent=Msa0065230-mRNA-1
chr1_2	CDS	27155640	27155838	27155640	ID=Msa0065230-mRNA-1:cds;Parent=Msa0065230-mRNA-1
chr1_2	CDS	27156255	27156460	27156255	ID=Msa0065230-mRNA-1:cds;Parent=Msa0065230-mRNA-1

Gene Sequence

>Msa0065230

ATGGTTTTGACAGAAACTAAACTTTGGCGTAAAGGACCGTATGAGAAGCCAATACTATGTAATGCTTGTGGACTACATTTCAAGGCTAAAGGAACCCTTGATAACTATCTTCCGAAGACATCCCAGCAAAATCAGTTATCAGACCATGACGTTGAAAAGGAATCTGACAGTGATCCCGAGCTTTCAAACCAATCTCCAGAAAGGACACCATCGATTGTGAAGAATCAAATGGAGGAGTTTCAACAAAAGCTTCAAAGTCTATGGGAATCTGAGAAAGAATCAAATGAATCATCTGAAGACAATATTTTGTTGTTTGATAATATGAACAACTGTATACCAAGCAATGAGATAGGCATTGGATGCACTCTACTAAAGACAGATGTTACTTCTAAAAAATCTGCATGA

Protein sequence

>Msa0065230

MVLTETKLWRKGPYEKPILCNACGLHFKAKGTLDNYLPKTSQQNQLSDHDVEKESDSDPELSNQSPERTPSIVKNQMEEFQQKLQSLWESEKESNESSEDNILLFDNMNNCIPSNEIGIGCTLLKTDVTSKKSA*