Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0065230 | G7I6L4 | 86.567 | 134 | 18 | 0 | 1 | 134 | 1 | 134 | 9.81e-74 | 225 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0065230 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0065230 | MtrunA17_Chr5g0399781 | 52.830 | 53 | 24 | 1 | 3 | 55 | 12 | 63 | 4.50e-11 | 58.5 |
| Msa0065230 | MtrunA17_Chr4g0055611 | 66.667 | 36 | 12 | 0 | 3 | 38 | 12 | 47 | 6.46e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0065230 | AT5G47140.1 | 38.889 | 72 | 40 | 1 | 3 | 70 | 12 | 83 | 4.13e-11 | 59.7 |
Find 20 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1_2:+27156297 | Msa0065230:CDS |
| CCACAAGCATTACATAGTAT+TGG | 0.433061 | 1_2:-27155688 | None:intergenic |
| TGGAGATTGGTTTGAAAGCT+CGG | 0.447768 | 1_2:-27155815 | None:intergenic |
| GAAACTAAACTTTGGCGTAA+AGG | 0.456777 | 1_2:+27155652 | Msa0065230:CDS |
| TGCTTGTGGACTACATTTCA+AGG | 0.461822 | 1_2:+27155702 | Msa0065230:CDS |
| GGACTACATTTCAAGGCTAA+AGG | 0.474721 | 1_2:+27155709 | Msa0065230:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1_2:-27155735 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.478624 | 1_2:+27156386 | Msa0065230:CDS |
| ATCAGACCATGACGTTGAAA+AGG | 0.486360 | 1_2:+27155777 | Msa0065230:CDS |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1_2:+27156265 | Msa0065230:CDS |
| CATAGTATTGGCTTCTCATA+CGG | 0.492997 | 1_2:-27155676 | None:intergenic |
| GGAGATTGGTTTGAAAGCTC+GGG | 0.493147 | 1_2:-27155814 | None:intergenic |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1_2:+27156392 | Msa0065230:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1_2:-27156263 | None:intergenic |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1_2:+27156298 | Msa0065230:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1_2:-27156392 | None:intergenic |
| GAGCTTTCAAACCAATCTCC+AGG | 0.568278 | 1_2:+27155817 | Msa0065230:CDS |
| CCAATACTATGTAATGCTTG+TGG | 0.579274 | 1_2:+27155688 | Msa0065230:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1_2:-27155734 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1_2:+27156268 | Msa0065230:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATATTTGTTGAAAATTG+GGG | - | chr1_2:27155877-27155896 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAATT+GGG | - | chr1_2:27155878-27155897 | None:intergenic | 15.0% |
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1_2:27156237-27156256 | Msa0065230:intron | 15.0% |
| !!! | TTTATATTTGTTGAAAATTG+GGG | - | chr1_2:27155877-27155896 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAATT+GGG | - | chr1_2:27155878-27155897 | None:intergenic | 15.0% |
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1_2:27156237-27156256 | Msa0065230:intron | 15.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_2:27155838-27155857 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAT+TGG | - | chr1_2:27155879-27155898 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_2:27155932-27155951 | Msa0065230:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_2:27155983-27156002 | Msa0065230:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_2:27156076-27156095 | Msa0065230:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_2:27156077-27156096 | Msa0065230:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_2:27156217-27156236 | None:intergenic | 20.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_2:27155838-27155857 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAT+TGG | - | chr1_2:27155879-27155898 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_2:27155932-27155951 | Msa0065230:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_2:27155983-27156002 | Msa0065230:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_2:27156076-27156095 | Msa0065230:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_2:27156077-27156096 | Msa0065230:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_2:27156217-27156236 | None:intergenic | 20.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_2:27155644-27155663 | Msa0065230:CDS | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_2:27155831-27155850 | None:intergenic | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_2:27155943-27155962 | Msa0065230:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_2:27156023-27156042 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_2:27156024-27156043 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_2:27156078-27156097 | Msa0065230:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_2:27156126-27156145 | None:intergenic | 25.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_2:27155644-27155663 | Msa0065230:CDS | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_2:27155831-27155850 | None:intergenic | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_2:27155943-27155962 | Msa0065230:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_2:27156023-27156042 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_2:27156024-27156043 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_2:27156078-27156097 | Msa0065230:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_2:27156126-27156145 | None:intergenic | 25.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_2:27156031-27156050 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_2:27156120-27156139 | Msa0065230:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_2:27156266-27156285 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_2:27156265-27156284 | Msa0065230:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_2:27156297-27156316 | Msa0065230:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_2:27156298-27156317 | Msa0065230:CDS | 30.0% | |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_2:27156031-27156050 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_2:27156120-27156139 | Msa0065230:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_2:27156266-27156285 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_2:27156265-27156284 | Msa0065230:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_2:27156297-27156316 | Msa0065230:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_2:27156298-27156317 | Msa0065230:CDS | 30.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_2:27155652-27155671 | Msa0065230:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_2:27155679-27155698 | None:intergenic | 35.0% |
| CCACAAGCATTACATAGTAT+TGG | - | chr1_2:27155691-27155710 | None:intergenic | 35.0% | |
| CCAATACTATGTAATGCTTG+TGG | + | chr1_2:27155688-27155707 | Msa0065230:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_2:27155737-27155756 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_2:27155738-27155757 | None:intergenic | 35.0% | |
| !! | GTCTGATAACTGATTTTGCT+GGG | - | chr1_2:27155764-27155783 | None:intergenic | 35.0% |
| AGCATTCATTTGAGTAGACA+CGG | + | chr1_2:27156046-27156065 | Msa0065230:intron | 35.0% | |
| ATTTGAGTAGACACGGTATT+AGG | + | chr1_2:27156053-27156072 | Msa0065230:intron | 35.0% | |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_2:27156084-27156103 | Msa0065230:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_2:27156204-27156223 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_2:27156268-27156287 | Msa0065230:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_2:27156386-27156405 | Msa0065230:CDS | 35.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_2:27155652-27155671 | Msa0065230:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_2:27155679-27155698 | None:intergenic | 35.0% |
| CCACAAGCATTACATAGTAT+TGG | - | chr1_2:27155691-27155710 | None:intergenic | 35.0% | |
| CCAATACTATGTAATGCTTG+TGG | + | chr1_2:27155688-27155707 | Msa0065230:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_2:27155737-27155756 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_2:27155738-27155757 | None:intergenic | 35.0% | |
| !! | GTCTGATAACTGATTTTGCT+GGG | - | chr1_2:27155764-27155783 | None:intergenic | 35.0% |
| AGCATTCATTTGAGTAGACA+CGG | + | chr1_2:27156046-27156065 | Msa0065230:intron | 35.0% | |
| ATTTGAGTAGACACGGTATT+AGG | + | chr1_2:27156053-27156072 | Msa0065230:intron | 35.0% | |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_2:27156084-27156103 | Msa0065230:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_2:27156204-27156223 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_2:27156268-27156287 | Msa0065230:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_2:27156386-27156405 | Msa0065230:CDS | 35.0% | |
| ! | TGCTTGTGGACTACATTTCA+AGG | + | chr1_2:27155702-27155721 | Msa0065230:CDS | 40.0% |
| GGACTACATTTCAAGGCTAA+AGG | + | chr1_2:27155709-27155728 | Msa0065230:CDS | 40.0% | |
| ! | TGATTTTGCTGGGATGTCTT+CGG | - | chr1_2:27155754-27155773 | None:intergenic | 40.0% |
| !! | GGTCTGATAACTGATTTTGC+TGG | - | chr1_2:27155765-27155784 | None:intergenic | 40.0% |
| ATCAGACCATGACGTTGAAA+AGG | + | chr1_2:27155777-27155796 | Msa0065230:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+TGG | - | chr1_2:27155786-27155805 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_2:27155818-27155837 | None:intergenic | 40.0% |
| ! | TGCTTGTGGACTACATTTCA+AGG | + | chr1_2:27155702-27155721 | Msa0065230:CDS | 40.0% |
| GGACTACATTTCAAGGCTAA+AGG | + | chr1_2:27155709-27155728 | Msa0065230:CDS | 40.0% | |
| ! | TGATTTTGCTGGGATGTCTT+CGG | - | chr1_2:27155754-27155773 | None:intergenic | 40.0% |
| !! | GGTCTGATAACTGATTTTGC+TGG | - | chr1_2:27155765-27155784 | None:intergenic | 40.0% |
| ATCAGACCATGACGTTGAAA+AGG | + | chr1_2:27155777-27155796 | Msa0065230:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+TGG | - | chr1_2:27155786-27155805 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_2:27155818-27155837 | None:intergenic | 40.0% |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_2:27155817-27155836 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_2:27155817-27155836 | Msa0065230:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_2:27156395-27156414 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_2:27156392-27156411 | Msa0065230:CDS | 45.0% | |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_2:27155817-27155836 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_2:27155817-27155836 | Msa0065230:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_2:27156395-27156414 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_2:27156392-27156411 | Msa0065230:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 27155640 | 27156460 | 27155640 | ID=Msa0065230;Name=Msa0065230 |
| chr1_2 | mRNA | 27155640 | 27156460 | 27155640 | ID=Msa0065230-mRNA-1;Parent=Msa0065230;Name=Msa0065230-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|134 |
| chr1_2 | exon | 27155640 | 27155838 | 27155640 | ID=Msa0065230-mRNA-1:exon:9506;Parent=Msa0065230-mRNA-1 |
| chr1_2 | exon | 27156255 | 27156460 | 27156255 | ID=Msa0065230-mRNA-1:exon:9507;Parent=Msa0065230-mRNA-1 |
| chr1_2 | CDS | 27155640 | 27155838 | 27155640 | ID=Msa0065230-mRNA-1:cds;Parent=Msa0065230-mRNA-1 |
| chr1_2 | CDS | 27156255 | 27156460 | 27156255 | ID=Msa0065230-mRNA-1:cds;Parent=Msa0065230-mRNA-1 |
| Gene Sequence |
| Protein sequence |
