AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0067080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0067080 MtrunA17_Chr1g0170581 71.220 205 19 2 4 168 43 247 4.38e-92 268
Msa0067080 MtrunA17_Chr1g0170571 67.822 202 25 2 4 165 35 236 2.94e-85 250
Msa0067080 MtrunA17_Chr1g0170591 67.327 202 26 2 4 165 35 236 2.56e-84 248
Msa0067080 MtrunA17_Chr7g0256671 48.259 201 61 6 3 162 44 242 1.24e-34 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0067080 AT3G56970.1 36.585 164 80 2 3 144 49 210 5.99e-25 97.1
Msa0067080 AT3G56980.1 34.568 162 86 3 3 144 54 215 1.05e-16 75.5
Msa0067080 AT5G04150.2 37.241 145 61 4 21 140 78 217 1.27e-15 72.0
Msa0067080 AT5G04150.1 36.429 140 62 3 21 135 78 215 1.92e-15 72.0
Msa0067080 AT2G41240.1 29.834 181 84 3 24 163 60 238 7.88e-14 67.4
Msa0067080 AT2G41240.2 30.337 178 86 4 24 163 60 237 2.56e-13 65.9

Find 22 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATCAAGATCTCAGAAAC+TGG 0.369301 1_2:+31672407 None:intergenic
GATCGCGTTCACTAGCATTA+TGG 0.375108 1_2:+31672842 None:intergenic
TAGAATAACCTCTTCTACTT+GGG 0.395863 1_2:+31672547 None:intergenic
AGTACCATTTGATCTGAAAC+AGG 0.414053 1_2:+31672772 None:intergenic
TCTTCCTGTTTCAGATCAAA+TGG 0.439923 1_2:-31672776 Msa0067080:intron
TTAGAATAACCTCTTCTACT+TGG 0.448901 1_2:+31672546 None:intergenic
CATGCCAAGGTCACTGCTTG+TGG 0.499479 1_2:+31672879 None:intergenic
ACAAATTATTGATCTTCTTG+CGG 0.529574 1_2:+31672818 None:intergenic
ACTTTCTCCCCAAGTAGAAG+AGG 0.556979 1_2:-31672555 Msa0067080:CDS
CAATCCACAAGCAGTGACCT+TGG 0.569748 1_2:-31672883 Msa0067080:CDS
GCTCTTATCAATAATCGAGA+AGG 0.576892 1_2:-31671771 Msa0067080:CDS
TTGATGTGTTTAGAAAATGA+TGG 0.580377 1_2:-31672392 Msa0067080:CDS
AGAATAACCTCTTCTACTTG+GGG 0.586846 1_2:+31672548 None:intergenic
ACTAGTTAAGAGAAAAGAAG+AGG 0.589176 1_2:-31672588 Msa0067080:CDS
GTCACTGCTTGTGGATTGTG+AGG 0.591686 1_2:+31672888 None:intergenic
CACAAGCAGTGACCTTGGCA+TGG 0.602895 1_2:-31672878 Msa0067080:CDS
TTATCAATAATCGAGAAGGA+AGG 0.636620 1_2:-31671767 Msa0067080:CDS
CAAGCTTCTTAACCATGCCA+AGG 0.655760 1_2:+31672866 None:intergenic
GAATTACAGAAACAGGTGGA+AGG 0.659206 1_2:-31672611 Msa0067080:intron
TAAGCAAAGAATCACAAAGG+AGG 0.661765 1_2:-31672526 Msa0067080:CDS
TTCTAAGCAAAGAATCACAA+AGG 0.666729 1_2:-31672529 Msa0067080:CDS
TCACTGCTTGTGGATTGTGA+GGG 0.698792 1_2:+31672889 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAGTTTTTTTTTTTTAGAT+CGG + chr1_2:31671818-31671837 None:intergenic 15.0%
!! ATTAACAAAATCATATAGAA+GGG + chr1_2:31672357-31672376 None:intergenic 15.0%
!! AATTAACAAAATCATATAGA+AGG + chr1_2:31672358-31672377 None:intergenic 15.0%
!! GATCATATATAATTATGATT+TGG - chr1_2:31672919-31672938 Msa0067080:CDS 15.0%
!! TATACAAATAATTGATTACT+TGG - chr1_2:31673142-31673161 Msa0067080:intron 15.0%
!! ATACAAATAATTGATTACTT+GGG - chr1_2:31673143-31673162 Msa0067080:intron 15.0%
!!! ACAGTTTTTTTTTTTTAGAT+CGG + chr1_2:31671818-31671837 None:intergenic 15.0%
!! ATTAACAAAATCATATAGAA+GGG + chr1_2:31672357-31672376 None:intergenic 15.0%
!! AATTAACAAAATCATATAGA+AGG + chr1_2:31672358-31672377 None:intergenic 15.0%
!! GATCATATATAATTATGATT+TGG - chr1_2:31672919-31672938 Msa0067080:CDS 15.0%
!! TATACAAATAATTGATTACT+TGG - chr1_2:31673142-31673161 Msa0067080:intron 15.0%
!! ATACAAATAATTGATTACTT+GGG - chr1_2:31673143-31673162 Msa0067080:intron 15.0%
!!! CATTACTCTTTTGAAAAAAT+AGG + chr1_2:31671878-31671897 None:intergenic 20.0%
!!! TCATTTCTCTTTTCAAAAAT+TGG - chr1_2:31672065-31672084 Msa0067080:intron 20.0%
!! TTAATAACATCTAAGAAGTA+AGG - chr1_2:31673083-31673102 Msa0067080:intron 20.0%
!!! ACACAAATAGCTTTTAATTA+TGG + chr1_2:31673251-31673270 None:intergenic 20.0%
!!! CATTACTCTTTTGAAAAAAT+AGG + chr1_2:31671878-31671897 None:intergenic 20.0%
!!! TCATTTCTCTTTTCAAAAAT+TGG - chr1_2:31672065-31672084 Msa0067080:intron 20.0%
!! TTAATAACATCTAAGAAGTA+AGG - chr1_2:31673083-31673102 Msa0067080:intron 20.0%
!!! ACACAAATAGCTTTTAATTA+TGG + chr1_2:31673251-31673270 None:intergenic 20.0%
!!! TACTCTTTTGAAAAAATAGG+AGG + chr1_2:31671875-31671894 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG + chr1_2:31671939-31671958 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG - chr1_2:31671938-31671957 Msa0067080:intron 25.0%
! TTCTTATTGGTCAAAAACAA+TGG - chr1_2:31671951-31671970 Msa0067080:intron 25.0%
!! TTCTCTTTTCAAAAATTGGA+TGG - chr1_2:31672069-31672088 Msa0067080:intron 25.0%
! ACAAATTATTGATCTTCTTG+CGG + chr1_2:31672280-31672299 None:intergenic 25.0%
!! GTAATTCTGGTATGTATTTT+AGG + chr1_2:31672471-31672490 None:intergenic 25.0%
!! ATTTTGTAGTTTCAACTAGT+AGG - chr1_2:31672611-31672630 Msa0067080:intron 25.0%
! TTGATGTGTTTAGAAAATGA+TGG - chr1_2:31672703-31672722 Msa0067080:intron 25.0%
!! AATCTATAATTTGCATTTCC+AGG - chr1_2:31672751-31672770 Msa0067080:intron 25.0%
!!! TACTCTTTTGAAAAAATAGG+AGG + chr1_2:31671875-31671894 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG + chr1_2:31671939-31671958 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG - chr1_2:31671938-31671957 Msa0067080:intron 25.0%
! TTCTTATTGGTCAAAAACAA+TGG - chr1_2:31671951-31671970 Msa0067080:intron 25.0%
!! TTCTCTTTTCAAAAATTGGA+TGG - chr1_2:31672069-31672088 Msa0067080:intron 25.0%
! ACAAATTATTGATCTTCTTG+CGG + chr1_2:31672280-31672299 None:intergenic 25.0%
!! GTAATTCTGGTATGTATTTT+AGG + chr1_2:31672471-31672490 None:intergenic 25.0%
!! ATTTTGTAGTTTCAACTAGT+AGG - chr1_2:31672611-31672630 Msa0067080:intron 25.0%
! TTGATGTGTTTAGAAAATGA+TGG - chr1_2:31672703-31672722 Msa0067080:intron 25.0%
!! AATCTATAATTTGCATTTCC+AGG - chr1_2:31672751-31672770 Msa0067080:intron 25.0%
TCAACAAAGTTGTCATCAAA+TGG + chr1_2:31671913-31671932 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG - chr1_2:31671964-31671983 Msa0067080:intron 30.0%
!!! AACCACTTTTTGACAACTTT+TGG + chr1_2:31672036-31672055 None:intergenic 30.0%
! TCATTTTCAGCATCAATTTG+AGG - chr1_2:31672163-31672182 Msa0067080:intron 30.0%
ACTAGTTAAGAGAAAAGAAG+AGG - chr1_2:31672507-31672526 Msa0067080:CDS 30.0%
TAGAATAACCTCTTCTACTT+GGG + chr1_2:31672551-31672570 None:intergenic 30.0%
TTAGAATAACCTCTTCTACT+TGG + chr1_2:31672552-31672571 None:intergenic 30.0%
TTCTAAGCAAAGAATCACAA+AGG - chr1_2:31672566-31672585 Msa0067080:CDS 30.0%
GATGTATAGACTCATAAAAG+AGG - chr1_2:31673210-31673229 Msa0067080:intron 30.0%
TTATCAATAATCGAGAAGGA+AGG - chr1_2:31673328-31673347 Msa0067080:intron 30.0%
TCAACAAAGTTGTCATCAAA+TGG + chr1_2:31671913-31671932 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG - chr1_2:31671964-31671983 Msa0067080:intron 30.0%
!!! AACCACTTTTTGACAACTTT+TGG + chr1_2:31672036-31672055 None:intergenic 30.0%
! TCATTTTCAGCATCAATTTG+AGG - chr1_2:31672163-31672182 Msa0067080:intron 30.0%
ACTAGTTAAGAGAAAAGAAG+AGG - chr1_2:31672507-31672526 Msa0067080:CDS 30.0%
TAGAATAACCTCTTCTACTT+GGG + chr1_2:31672551-31672570 None:intergenic 30.0%
TTAGAATAACCTCTTCTACT+TGG + chr1_2:31672552-31672571 None:intergenic 30.0%
TTCTAAGCAAAGAATCACAA+AGG - chr1_2:31672566-31672585 Msa0067080:CDS 30.0%
GATGTATAGACTCATAAAAG+AGG - chr1_2:31673210-31673229 Msa0067080:intron 30.0%
TTATCAATAATCGAGAAGGA+AGG - chr1_2:31673328-31673347 Msa0067080:intron 30.0%
TGACATATTGATGGAGTAGT+TGG + chr1_2:31671843-31671862 None:intergenic 35.0%
AGATATCGCTGACATATTGA+TGG + chr1_2:31671852-31671871 None:intergenic 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG - chr1_2:31672031-31672050 Msa0067080:intron 35.0%
!! ATGGATTTTGATGCTGGTTA+TGG + chr1_2:31672115-31672134 None:intergenic 35.0%
!! TGTAGAATGGATTTTGATGC+TGG + chr1_2:31672121-31672140 None:intergenic 35.0%
GCATCAAAATCCATTCTACA+AGG - chr1_2:31672121-31672140 Msa0067080:intron 35.0%
TCAGTAATGTCCTTGTAGAA+TGG + chr1_2:31672134-31672153 None:intergenic 35.0%
TCTTCCTGTTTCAGATCAAA+TGG - chr1_2:31672319-31672338 Msa0067080:intron 35.0%
! AGTACCATTTGATCTGAAAC+AGG + chr1_2:31672326-31672345 None:intergenic 35.0%
CATACCAGAATTACAGAAAC+AGG - chr1_2:31672477-31672496 Msa0067080:CDS 35.0%
AGAATAACCTCTTCTACTTG+GGG + chr1_2:31672550-31672569 None:intergenic 35.0%
TAAGCAAAGAATCACAAAGG+AGG - chr1_2:31672569-31672588 Msa0067080:CDS 35.0%
! AAGCTATTTGTGTTTTCTGC+AGG - chr1_2:31673258-31673277 Msa0067080:intron 35.0%
GCTCTTATCAATAATCGAGA+AGG - chr1_2:31673324-31673343 Msa0067080:intron 35.0%
TGACATATTGATGGAGTAGT+TGG + chr1_2:31671843-31671862 None:intergenic 35.0%
AGATATCGCTGACATATTGA+TGG + chr1_2:31671852-31671871 None:intergenic 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG - chr1_2:31672031-31672050 Msa0067080:intron 35.0%
!! ATGGATTTTGATGCTGGTTA+TGG + chr1_2:31672115-31672134 None:intergenic 35.0%
!! TGTAGAATGGATTTTGATGC+TGG + chr1_2:31672121-31672140 None:intergenic 35.0%
GCATCAAAATCCATTCTACA+AGG - chr1_2:31672121-31672140 Msa0067080:intron 35.0%
TCAGTAATGTCCTTGTAGAA+TGG + chr1_2:31672134-31672153 None:intergenic 35.0%
TCTTCCTGTTTCAGATCAAA+TGG - chr1_2:31672319-31672338 Msa0067080:intron 35.0%
! AGTACCATTTGATCTGAAAC+AGG + chr1_2:31672326-31672345 None:intergenic 35.0%
CATACCAGAATTACAGAAAC+AGG - chr1_2:31672477-31672496 Msa0067080:CDS 35.0%
AGAATAACCTCTTCTACTTG+GGG + chr1_2:31672550-31672569 None:intergenic 35.0%
TAAGCAAAGAATCACAAAGG+AGG - chr1_2:31672569-31672588 Msa0067080:CDS 35.0%
! AAGCTATTTGTGTTTTCTGC+AGG - chr1_2:31673258-31673277 Msa0067080:intron 35.0%
GCTCTTATCAATAATCGAGA+AGG - chr1_2:31673324-31673343 Msa0067080:intron 35.0%
AGAGAGAAAAAGGAAGAGAG+AGG - chr1_2:31671974-31671993 Msa0067080:intron 40.0%
!! TGGCTTATTGGCTCTTCAAA+GGG + chr1_2:31672095-31672114 None:intergenic 40.0%
!! ATGGCTTATTGGCTCTTCAA+AGG + chr1_2:31672096-31672115 None:intergenic 40.0%
! TGATGCTGGTTATGGCTTAT+TGG + chr1_2:31672107-31672126 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG + chr1_2:31672451-31672470 None:intergenic 40.0%
ACCAGAATTACAGAAACAGG+TGG - chr1_2:31672480-31672499 Msa0067080:CDS 40.0%
!! TCCACCTGTTTCTGTAATTC+TGG + chr1_2:31672484-31672503 None:intergenic 40.0%
GAATTACAGAAACAGGTGGA+AGG - chr1_2:31672484-31672503 Msa0067080:CDS 40.0%
CACATCAAGATCTCAGAAAC+TGG + chr1_2:31672691-31672710 None:intergenic 40.0%
CCTAGTGAAATTAACACACC+TGG + chr1_2:31672772-31672791 None:intergenic 40.0%
CCAGGTGTGTTAATTTCACT+AGG - chr1_2:31672769-31672788 Msa0067080:intron 40.0%
! CTATTTGTGTTTTCTGCAGG+TGG - chr1_2:31673261-31673280 Msa0067080:intron 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG - chr1_2:31671974-31671993 Msa0067080:intron 40.0%
!! TGGCTTATTGGCTCTTCAAA+GGG + chr1_2:31672095-31672114 None:intergenic 40.0%
!! ATGGCTTATTGGCTCTTCAA+AGG + chr1_2:31672096-31672115 None:intergenic 40.0%
! TGATGCTGGTTATGGCTTAT+TGG + chr1_2:31672107-31672126 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG + chr1_2:31672451-31672470 None:intergenic 40.0%
ACCAGAATTACAGAAACAGG+TGG - chr1_2:31672480-31672499 Msa0067080:CDS 40.0%
!! TCCACCTGTTTCTGTAATTC+TGG + chr1_2:31672484-31672503 None:intergenic 40.0%
GAATTACAGAAACAGGTGGA+AGG - chr1_2:31672484-31672503 Msa0067080:CDS 40.0%
CACATCAAGATCTCAGAAAC+TGG + chr1_2:31672691-31672710 None:intergenic 40.0%
CCTAGTGAAATTAACACACC+TGG + chr1_2:31672772-31672791 None:intergenic 40.0%
CCAGGTGTGTTAATTTCACT+AGG - chr1_2:31672769-31672788 Msa0067080:intron 40.0%
! CTATTTGTGTTTTCTGCAGG+TGG - chr1_2:31673261-31673280 Msa0067080:intron 40.0%
! TCACTGCTTGTGGATTGTGA+GGG + chr1_2:31672209-31672228 None:intergenic 45.0%
CAAGCTTCTTAACCATGCCA+AGG + chr1_2:31672232-31672251 None:intergenic 45.0%
GATCGCGTTCACTAGCATTA+TGG + chr1_2:31672256-31672275 None:intergenic 45.0%
ACTTTCTCCCCAAGTAGAAG+AGG - chr1_2:31672540-31672559 Msa0067080:CDS 45.0%
! TCACTGCTTGTGGATTGTGA+GGG + chr1_2:31672209-31672228 None:intergenic 45.0%
CAAGCTTCTTAACCATGCCA+AGG + chr1_2:31672232-31672251 None:intergenic 45.0%
GATCGCGTTCACTAGCATTA+TGG + chr1_2:31672256-31672275 None:intergenic 45.0%
ACTTTCTCCCCAAGTAGAAG+AGG - chr1_2:31672540-31672559 Msa0067080:CDS 45.0%
! GTCACTGCTTGTGGATTGTG+AGG + chr1_2:31672210-31672229 None:intergenic 50.0%
CAATCCACAAGCAGTGACCT+TGG - chr1_2:31672212-31672231 Msa0067080:intron 50.0%
! GTCACTGCTTGTGGATTGTG+AGG + chr1_2:31672210-31672229 None:intergenic 50.0%
CAATCCACAAGCAGTGACCT+TGG - chr1_2:31672212-31672231 Msa0067080:intron 50.0%
CATGCCAAGGTCACTGCTTG+TGG + chr1_2:31672219-31672238 None:intergenic 55.0%
CACAAGCAGTGACCTTGGCA+TGG - chr1_2:31672217-31672236 Msa0067080:intron 55.0%
CATGCCAAGGTCACTGCTTG+TGG + chr1_2:31672219-31672238 None:intergenic 55.0%
CACAAGCAGTGACCTTGGCA+TGG - chr1_2:31672217-31672236 Msa0067080:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 31671748 31673369 31671748 ID=Msa0067080;Name=Msa0067080
chr1_2 mRNA 31671748 31673369 31671748 ID=Msa0067080-mRNA-1;Parent=Msa0067080;Name=Msa0067080-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|0.75|0|0.25|4|0|168
chr1_2 exon 31673364 31673369 31673364 ID=Msa0067080-mRNA-1:exon:10445;Parent=Msa0067080-mRNA-1
chr1_2 exon 31672777 31672944 31672777 ID=Msa0067080-mRNA-1:exon:10444;Parent=Msa0067080-mRNA-1
chr1_2 exon 31672376 31672618 31672376 ID=Msa0067080-mRNA-1:exon:10443;Parent=Msa0067080-mRNA-1
chr1_2 exon 31671748 31671837 31671748 ID=Msa0067080-mRNA-1:exon:10442;Parent=Msa0067080-mRNA-1
chr1_2 CDS 31673364 31673369 31673364 ID=Msa0067080-mRNA-1:cds;Parent=Msa0067080-mRNA-1
chr1_2 CDS 31672777 31672944 31672777 ID=Msa0067080-mRNA-1:cds;Parent=Msa0067080-mRNA-1
chr1_2 CDS 31672376 31672618 31672376 ID=Msa0067080-mRNA-1:cds;Parent=Msa0067080-mRNA-1
chr1_2 CDS 31671748 31671837 31671748 ID=Msa0067080-mRNA-1:cds;Parent=Msa0067080-mRNA-1
Gene Sequence

>Msa0067080

ATGATGCATCAATTTGAGGCAGAAATTAATTTAACAGATCCCTCACAATCCACAAGCAGTGACCTTGGCATGGTTAAGAAGCTTGTCCATAATGCTAGTGAACGCGATCGCCGCAAGAAGATCAATAATTTGTATTCTTCTCTTCGTTCACTTCTTCCTGTTTCAGATCAAATGGTGGAAGGACTAGTTAAGAGAAAAGAAGAGGTCTTATTGAGACTTTCTCCCCAAGTAGAAGAGGTTATTCTAAGCAAAGAATCACAAAGGAGGAAGCATAGTTACAACTCTGATTTTGTAGTTTCAACTAGTAGGCTAAATGATAGTGAAGTTACTATTCAGATATCATGTTACACTGTCAACAAGATTCCAGTTTCTGAGATCTTGATGTGTTTAGAAAATGATGGCTTTTTGCTGCTTAATGTGGATAAAGCACAGAGATTAGAATCTCATATTCTAAATGAGAAGCTCTTATCAATAATCGAGAAGGAAGGAGAGTTTTTAAAACAATAA

Protein sequence

>Msa0067080

MMHQFEAEINLTDPSQSTSSDLGMVKKLVHNASERDRRKKINNLYSSLRSLLPVSDQMVEGLVKRKEEVLLRLSPQVEEVILSKESQRRKHSYNSDFVVSTSRLNDSEVTIQISCYTVNKIPVSEILMCLENDGFLLLNVDKAQRLESHILNEKLLSIIEKEGEFLKQ*