AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0067110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0067110 MtrunA17_Chr1g0170581 85.656 244 35 0 1 244 1 244 3.81e-153 426
Msa0067110 MtrunA17_Chr1g0170591 84.836 244 29 1 1 244 1 236 3.74e-150 418
Msa0067110 MtrunA17_Chr1g0170571 83.333 246 33 1 1 246 1 238 3.71e-140 392
Msa0067110 MtrunA17_Chr7g0256671 56.972 251 89 7 1 241 1 242 8.27e-62 194
Msa0067110 MtrunA17_Chr6g0474681 32.680 153 88 5 65 212 6 148 5.04e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0067110 AT3G56970.1 39.442 251 137 6 1 240 1 247 1.84e-49 163
Msa0067110 AT3G56980.1 38.462 273 118 6 1 242 1 254 2.05e-41 142
Msa0067110 AT2G41240.1 36.437 247 143 4 1 242 1 238 2.16e-40 139
Msa0067110 AT2G41240.2 36.032 247 143 5 1 242 1 237 7.00e-38 133
Msa0067110 AT5G04150.1 41.081 185 101 4 60 239 78 259 3.58e-32 119
Msa0067110 AT5G04150.2 42.945 163 88 2 60 219 78 238 3.61e-32 118

Find 40 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACATGTTTCTGTATTTC+TGG 0.048296 1_2:+31710184 None:intergenic
TGATGCTGGTTATGGCTTAT+TGG 0.196697 1_2:+31710685 None:intergenic
AGTACCATTTGATCAGAAAT+AGG 0.267145 1_2:+31710466 None:intergenic
TGAGAAGCTGTTATCAATAA+TGG 0.294727 1_2:-31709742 Msa0067110:CDS
ATGGATTCTGATGCTGGTTA+TGG 0.357369 1_2:+31710677 None:intergenic
AGGACTCTTGAAATTGTTGC+TGG 0.367920 1_2:+31710217 None:intergenic
TCAATAGTGTCTTTGTAGAA+TGG 0.399191 1_2:+31710658 None:intergenic
TCTTCCTATTTCTGATCAAA+TGG 0.404052 1_2:-31710470 Msa0067110:intron
ATGGCTTATTGGCTCTTCAA+AGG 0.435523 1_2:+31710696 None:intergenic
ATTTCTTCATCTCAAACCTA+TGG 0.440745 1_2:-31709923 Msa0067110:CDS
TGTAGAATGGATTCTGATGC+TGG 0.456368 1_2:+31710671 None:intergenic
AAGAAATATTAAGCAGCAAA+AGG 0.464396 1_2:+31709939 None:intergenic
GCTGTTATCAATAATGGAAA+AGG 0.470141 1_2:-31709736 Msa0067110:CDS
TGGCTTATTGGCTCTTCAAA+GGG 0.488560 1_2:+31710697 None:intergenic
GCAGAAGACCCTCCCTCCAT+AGG 0.512036 1_2:+31709907 None:intergenic
ATATTAACTTCATTTACTTG+TGG 0.516009 1_2:+31710118 None:intergenic
CACATCAGGATCTTAGAAAG+TGG 0.517114 1_2:+31709977 None:intergenic
ACAAATTATTCATCTTCTTG+CGG 0.523820 1_2:+31710512 None:intergenic
TCTTCATCTCAAACCTATGG+AGG 0.533541 1_2:-31709920 Msa0067110:CDS
AAGAAACATAGTTACAACTC+TGG 0.547575 1_2:-31710076 Msa0067110:CDS
CAATAATGGAAAAGGAAGGA+GGG 0.549179 1_2:-31709728 Msa0067110:CDS
TCAATAATGGAAAAGGAAGG+AGG 0.551798 1_2:-31709729 Msa0067110:CDS
CATGCCAAGGTCACTGCTTG+TGG 0.551876 1_2:+31710573 None:intergenic
CATCCAATTGTTGAAAACAT+AGG 0.558093 1_2:+31710721 None:intergenic
CAATCCACAAGCAGTGACCT+TGG 0.569748 1_2:-31710577 Msa0067110:CDS
ATCTCAAACCTATGGAGGGA+GGG 0.571867 1_2:-31709915 Msa0067110:CDS
AGTGTAACATGAAATGTGAA+TGG 0.579724 1_2:+31710012 None:intergenic
CCAATTGTTGAAAACATAGG+AGG 0.582074 1_2:+31710724 None:intergenic
TCTTAGAAAGTGGAATCTTG+TGG 0.583213 1_2:+31709987 None:intergenic
CTTCATCTCAAACCTATGGA+GGG 0.583617 1_2:-31709919 Msa0067110:CDS
GTCACTGCTTGTGGATTGTG+AGG 0.591686 1_2:+31710582 None:intergenic
CACAAGCAGTGACCTTGGCA+TGG 0.603384 1_2:-31710572 Msa0067110:CDS
CAGAAATAGGAAGAAGTGAA+CGG 0.605298 1_2:+31710479 None:intergenic
CATCTCAAACCTATGGAGGG+AGG 0.626178 1_2:-31709916 Msa0067110:CDS
GAAATACAGAAACATGTGGA+AGG 0.627895 1_2:-31710181 Msa0067110:CDS
TTATCAATAATGGAAAAGGA+AGG 0.643476 1_2:-31709732 Msa0067110:CDS
ACCAGAAATACAGAAACATG+TGG 0.649518 1_2:-31710185 Msa0067110:CDS
CTGATGTGTTTAGAAAATGA+TGG 0.652952 1_2:-31709962 Msa0067110:CDS
CAAGCTTCTTGACCATGCCA+AGG 0.656633 1_2:+31710560 None:intergenic
TCACTGCTTGTGGATTGTGA+GGG 0.698792 1_2:+31710583 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAAGTAATATCGAAAAA+CGG + chr1_2:31710136-31710155 None:intergenic 15.0%
!!! TTTAAAGTAATATCGAAAAA+CGG + chr1_2:31710136-31710155 None:intergenic 15.0%
!! TTCGATATTACTTTAAATTG+TGG - chr1_2:31710139-31710158 Msa0067110:CDS 20.0%
!! ATATTAACTTCATTTACTTG+TGG + chr1_2:31710337-31710356 None:intergenic 20.0%
!! TTCGATATTACTTTAAATTG+TGG - chr1_2:31710139-31710158 Msa0067110:CDS 20.0%
!! ATATTAACTTCATTTACTTG+TGG + chr1_2:31710337-31710356 None:intergenic 20.0%
! ACAAATTATTCATCTTCTTG+CGG + chr1_2:31709943-31709962 None:intergenic 25.0%
! ACAATTAGAAACAGAAAGAA+GGG + chr1_2:31710020-31710039 None:intergenic 25.0%
!!! GTATTTCTGGTATGTATTTT+AGG + chr1_2:31710258-31710277 None:intergenic 25.0%
! AAGAAATATTAAGCAGCAAA+AGG + chr1_2:31710516-31710535 None:intergenic 25.0%
!! ATTCAACTAAAAGCTTCTTT+TGG - chr1_2:31710638-31710657 Msa0067110:CDS 25.0%
!! TTCAACTAAAAGCTTCTTTT+GGG - chr1_2:31710639-31710658 Msa0067110:CDS 25.0%
!! TTATCAATAATGGAAAAGGA+AGG - chr1_2:31710720-31710739 Msa0067110:CDS 25.0%
! ACAAATTATTCATCTTCTTG+CGG + chr1_2:31709943-31709962 None:intergenic 25.0%
! ACAATTAGAAACAGAAAGAA+GGG + chr1_2:31710020-31710039 None:intergenic 25.0%
!!! GTATTTCTGGTATGTATTTT+AGG + chr1_2:31710258-31710277 None:intergenic 25.0%
! AAGAAATATTAAGCAGCAAA+AGG + chr1_2:31710516-31710535 None:intergenic 25.0%
!! ATTCAACTAAAAGCTTCTTT+TGG - chr1_2:31710638-31710657 Msa0067110:CDS 25.0%
!! TTCAACTAAAAGCTTCTTTT+GGG - chr1_2:31710639-31710658 Msa0067110:CDS 25.0%
!! TTATCAATAATGGAAAAGGA+AGG - chr1_2:31710720-31710739 Msa0067110:CDS 25.0%
CATCCAATTGTTGAAAACAT+AGG + chr1_2:31709734-31709753 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG - chr1_2:31709732-31709751 Msa0067110:CDS 30.0%
TCAATAGTGTCTTTGTAGAA+TGG + chr1_2:31709797-31709816 None:intergenic 30.0%
!! TCATTTTGATGATCAAGTAG+AGG - chr1_2:31709826-31709845 Msa0067110:intron 30.0%
!! TCTTCCTATTTCTGATCAAA+TGG - chr1_2:31709982-31710001 Msa0067110:CDS 30.0%
AGTACCATTTGATCAGAAAT+AGG + chr1_2:31709989-31710008 None:intergenic 30.0%
GACAATTAGAAACAGAAAGA+AGG + chr1_2:31710021-31710040 None:intergenic 30.0%
!!! GTTTTGTTTTGACATGAGTA+TGG - chr1_2:31710088-31710107 Msa0067110:CDS 30.0%
TATGGTTGCAATTTCCTAAT+TGG - chr1_2:31710106-31710125 Msa0067110:CDS 30.0%
ATGGTTGCAATTTCCTAATT+GGG - chr1_2:31710107-31710126 Msa0067110:CDS 30.0%
AAGAAACATAGTTACAACTC+TGG - chr1_2:31710376-31710395 Msa0067110:intron 30.0%
! GTTTTGTAGTTTCAACAAGT+AGG - chr1_2:31710398-31710417 Msa0067110:intron 30.0%
AGTGTAACATGAAATGTGAA+TGG + chr1_2:31710443-31710462 None:intergenic 30.0%
! CATCATTTTCTAAACACATC+AGG + chr1_2:31710492-31710511 None:intergenic 30.0%
CTGATGTGTTTAGAAAATGA+TGG - chr1_2:31710490-31710509 Msa0067110:CDS 30.0%
ATTTCTTCATCTCAAACCTA+TGG - chr1_2:31710529-31710548 Msa0067110:CDS 30.0%
TGAGAAGCTGTTATCAATAA+TGG - chr1_2:31710710-31710729 Msa0067110:CDS 30.0%
! GCTGTTATCAATAATGGAAA+AGG - chr1_2:31710716-31710735 Msa0067110:CDS 30.0%
CATCCAATTGTTGAAAACAT+AGG + chr1_2:31709734-31709753 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG - chr1_2:31709732-31709751 Msa0067110:CDS 30.0%
TCAATAGTGTCTTTGTAGAA+TGG + chr1_2:31709797-31709816 None:intergenic 30.0%
!! TCATTTTGATGATCAAGTAG+AGG - chr1_2:31709826-31709845 Msa0067110:intron 30.0%
!! TCTTCCTATTTCTGATCAAA+TGG - chr1_2:31709982-31710001 Msa0067110:CDS 30.0%
AGTACCATTTGATCAGAAAT+AGG + chr1_2:31709989-31710008 None:intergenic 30.0%
GACAATTAGAAACAGAAAGA+AGG + chr1_2:31710021-31710040 None:intergenic 30.0%
!!! GTTTTGTTTTGACATGAGTA+TGG - chr1_2:31710088-31710107 Msa0067110:CDS 30.0%
TATGGTTGCAATTTCCTAAT+TGG - chr1_2:31710106-31710125 Msa0067110:CDS 30.0%
ATGGTTGCAATTTCCTAATT+GGG - chr1_2:31710107-31710126 Msa0067110:CDS 30.0%
AAGAAACATAGTTACAACTC+TGG - chr1_2:31710376-31710395 Msa0067110:intron 30.0%
! GTTTTGTAGTTTCAACAAGT+AGG - chr1_2:31710398-31710417 Msa0067110:intron 30.0%
AGTGTAACATGAAATGTGAA+TGG + chr1_2:31710443-31710462 None:intergenic 30.0%
! CATCATTTTCTAAACACATC+AGG + chr1_2:31710492-31710511 None:intergenic 30.0%
CTGATGTGTTTAGAAAATGA+TGG - chr1_2:31710490-31710509 Msa0067110:CDS 30.0%
ATTTCTTCATCTCAAACCTA+TGG - chr1_2:31710529-31710548 Msa0067110:CDS 30.0%
TGAGAAGCTGTTATCAATAA+TGG - chr1_2:31710710-31710729 Msa0067110:CDS 30.0%
! GCTGTTATCAATAATGGAAA+AGG - chr1_2:31710716-31710735 Msa0067110:CDS 30.0%
CCAATTGTTGAAAACATAGG+AGG + chr1_2:31709731-31709750 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG - chr1_2:31709728-31709747 Msa0067110:CDS 35.0%
CAGAAATAGGAAGAAGTGAA+CGG + chr1_2:31709976-31709995 None:intergenic 35.0%
ACCAGAAATACAGAAACATG+TGG - chr1_2:31710267-31710286 Msa0067110:intron 35.0%
! TCCACATGTTTCTGTATTTC+TGG + chr1_2:31710271-31710290 None:intergenic 35.0%
GAAATACAGAAACATGTGGA+AGG - chr1_2:31710271-31710290 Msa0067110:intron 35.0%
TCTTAGAAAGTGGAATCTTG+TGG + chr1_2:31710468-31710487 None:intergenic 35.0%
! TCAATAATGGAAAAGGAAGG+AGG - chr1_2:31710723-31710742 Msa0067110:CDS 35.0%
! CAATAATGGAAAAGGAAGGA+GGG - chr1_2:31710724-31710743 Msa0067110:CDS 35.0%
CCAATTGTTGAAAACATAGG+AGG + chr1_2:31709731-31709750 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG - chr1_2:31709728-31709747 Msa0067110:CDS 35.0%
CAGAAATAGGAAGAAGTGAA+CGG + chr1_2:31709976-31709995 None:intergenic 35.0%
ACCAGAAATACAGAAACATG+TGG - chr1_2:31710267-31710286 Msa0067110:intron 35.0%
! TCCACATGTTTCTGTATTTC+TGG + chr1_2:31710271-31710290 None:intergenic 35.0%
GAAATACAGAAACATGTGGA+AGG - chr1_2:31710271-31710290 Msa0067110:intron 35.0%
TCTTAGAAAGTGGAATCTTG+TGG + chr1_2:31710468-31710487 None:intergenic 35.0%
! TCAATAATGGAAAAGGAAGG+AGG - chr1_2:31710723-31710742 Msa0067110:CDS 35.0%
! CAATAATGGAAAAGGAAGGA+GGG - chr1_2:31710724-31710743 Msa0067110:CDS 35.0%
!! TGGCTTATTGGCTCTTCAAA+GGG + chr1_2:31709758-31709777 None:intergenic 40.0%
!! ATGGCTTATTGGCTCTTCAA+AGG + chr1_2:31709759-31709778 None:intergenic 40.0%
! TGATGCTGGTTATGGCTTAT+TGG + chr1_2:31709770-31709789 None:intergenic 40.0%
! ATGGATTCTGATGCTGGTTA+TGG + chr1_2:31709778-31709797 None:intergenic 40.0%
! TGTAGAATGGATTCTGATGC+TGG + chr1_2:31709784-31709803 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG + chr1_2:31710238-31710257 None:intergenic 40.0%
CACATCAGGATCTTAGAAAG+TGG + chr1_2:31710478-31710497 None:intergenic 40.0%
TCTTCATCTCAAACCTATGG+AGG - chr1_2:31710532-31710551 Msa0067110:CDS 40.0%
CTTCATCTCAAACCTATGGA+GGG - chr1_2:31710533-31710552 Msa0067110:CDS 40.0%
! CTTCTGCAATTCGCATTTTC+AGG - chr1_2:31710561-31710580 Msa0067110:CDS 40.0%
!! TGGCTTATTGGCTCTTCAAA+GGG + chr1_2:31709758-31709777 None:intergenic 40.0%
!! ATGGCTTATTGGCTCTTCAA+AGG + chr1_2:31709759-31709778 None:intergenic 40.0%
! TGATGCTGGTTATGGCTTAT+TGG + chr1_2:31709770-31709789 None:intergenic 40.0%
! ATGGATTCTGATGCTGGTTA+TGG + chr1_2:31709778-31709797 None:intergenic 40.0%
! TGTAGAATGGATTCTGATGC+TGG + chr1_2:31709784-31709803 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG + chr1_2:31710238-31710257 None:intergenic 40.0%
CACATCAGGATCTTAGAAAG+TGG + chr1_2:31710478-31710497 None:intergenic 40.0%
TCTTCATCTCAAACCTATGG+AGG - chr1_2:31710532-31710551 Msa0067110:CDS 40.0%
CTTCATCTCAAACCTATGGA+GGG - chr1_2:31710533-31710552 Msa0067110:CDS 40.0%
! CTTCTGCAATTCGCATTTTC+AGG - chr1_2:31710561-31710580 Msa0067110:CDS 40.0%
! TCACTGCTTGTGGATTGTGA+GGG + chr1_2:31709872-31709891 None:intergenic 45.0%
CGAAAAACGGCATCCCAATT+AGG + chr1_2:31710123-31710142 None:intergenic 45.0%
ATCTCAAACCTATGGAGGGA+GGG - chr1_2:31710537-31710556 Msa0067110:CDS 45.0%
!!! GCTTCTTTTGGGTTTTCTGC+AGG - chr1_2:31710650-31710669 Msa0067110:CDS 45.0%
!!! TCTTTTGGGTTTTCTGCAGG+TGG - chr1_2:31710653-31710672 Msa0067110:CDS 45.0%
! TCACTGCTTGTGGATTGTGA+GGG + chr1_2:31709872-31709891 None:intergenic 45.0%
CGAAAAACGGCATCCCAATT+AGG + chr1_2:31710123-31710142 None:intergenic 45.0%
ATCTCAAACCTATGGAGGGA+GGG - chr1_2:31710537-31710556 Msa0067110:CDS 45.0%
!!! GCTTCTTTTGGGTTTTCTGC+AGG - chr1_2:31710650-31710669 Msa0067110:CDS 45.0%
!!! TCTTTTGGGTTTTCTGCAGG+TGG - chr1_2:31710653-31710672 Msa0067110:CDS 45.0%
! GTCACTGCTTGTGGATTGTG+AGG + chr1_2:31709873-31709892 None:intergenic 50.0%
CAATCCACAAGCAGTGACCT+TGG - chr1_2:31709875-31709894 Msa0067110:intron 50.0%
CAAGCTTCTTGACCATGCCA+AGG + chr1_2:31709895-31709914 None:intergenic 50.0%
CATCTCAAACCTATGGAGGG+AGG - chr1_2:31710536-31710555 Msa0067110:CDS 50.0%
! GTCACTGCTTGTGGATTGTG+AGG + chr1_2:31709873-31709892 None:intergenic 50.0%
CAATCCACAAGCAGTGACCT+TGG - chr1_2:31709875-31709894 Msa0067110:intron 50.0%
CAAGCTTCTTGACCATGCCA+AGG + chr1_2:31709895-31709914 None:intergenic 50.0%
CATCTCAAACCTATGGAGGG+AGG - chr1_2:31710536-31710555 Msa0067110:CDS 50.0%
CATGCCAAGGTCACTGCTTG+TGG + chr1_2:31709882-31709901 None:intergenic 55.0%
CACAAGCAGTGACCTTGGCA+TGG - chr1_2:31709880-31709899 Msa0067110:intron 55.0%
CATGCCAAGGTCACTGCTTG+TGG + chr1_2:31709882-31709901 None:intergenic 55.0%
CACAAGCAGTGACCTTGGCA+TGG - chr1_2:31709880-31709899 Msa0067110:intron 55.0%
GCAGAAGACCCTCCCTCCAT+AGG + chr1_2:31710548-31710567 None:intergenic 60.0%
GCAGAAGACCCTCCCTCCAT+AGG + chr1_2:31710548-31710567 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 31709713 31710761 31709713 ID=Msa0067110;Name=Msa0067110
chr1_2 mRNA 31709713 31710761 31709713 ID=Msa0067110-mRNA-1;Parent=Msa0067110;Name=Msa0067110-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|3|0|247
chr1_2 exon 31710471 31710761 31710471 ID=Msa0067110-mRNA-1:exon:10450;Parent=Msa0067110-mRNA-1
chr1_2 exon 31709892 31710254 31709892 ID=Msa0067110-mRNA-1:exon:10449;Parent=Msa0067110-mRNA-1
chr1_2 exon 31709713 31709802 31709713 ID=Msa0067110-mRNA-1:exon:10448;Parent=Msa0067110-mRNA-1
chr1_2 CDS 31710471 31710761 31710471 ID=Msa0067110-mRNA-1:cds;Parent=Msa0067110-mRNA-1
chr1_2 CDS 31709892 31710254 31709892 ID=Msa0067110-mRNA-1:cds;Parent=Msa0067110-mRNA-1
chr1_2 CDS 31709713 31709802 31709713 ID=Msa0067110-mRNA-1:cds;Parent=Msa0067110-mRNA-1
Gene Sequence

>Msa0067110

ATGTTAGCGATATCTCCTCCTATGTTTTCAACAATTGGATGGCCCTTTGAAGAGCCAATAAGCCATAACCAGCATCAGAATCCATTCTACAAAGACACTATTGATCAATTATTTCATTTTGATGATCAAGTAGAGGCAGAAATTAATTCAACAGATCCCTCACAATCCACAAGCAGTGACCTTGGCATGGTCAAGAAGCTTGTTCATAATGCTAGTGAACGTCATCGCCGCAAGAAGATGAATAATTTGTATTCTTCACTCCGTTCACTTCTTCCTATTTCTGATCAAATGAAGAAATTGAGTATTCCAGCAACAATTTCAAGAGTCCTAAAATACATACCAGAAATACAGAAACATGTGGAAGGACTAATTAAGAGAAAAGAAGAGATCTTACTGAGACTTTCTCCACAAGTAAATGAAGTTAATATAAGCAAAGAATCTCAAAAGAAGAAACATAGTTACAACTCTGGTTTTGTAGTTTCAACAAGTAGGCTCAATGATAGTGAAATTACCATTCACATTTCATGTTACACTGTCCACAAGATTCCACTTTCTAAGATCCTGATGTGTTTAGAAAATGATGGCCTTTTGCTGCTTAATATTTCTTCATCTCAAACCTATGGAGGGAGGGTCTTCTGCAATTCGCATTTTCAGGTGGATAAAGCGCAAAGATTAGAATTCGATATTTTAAATGAGAAGCTGTTATCAATAATGGAAAAGGAAGGAGGGTTTTCAATACAATAA

Protein sequence

>Msa0067110

MLAISPPMFSTIGWPFEEPISHNQHQNPFYKDTIDQLFHFDDQVEAEINSTDPSQSTSSDLGMVKKLVHNASERHRRKKMNNLYSSLRSLLPISDQMKKLSIPATISRVLKYIPEIQKHVEGLIKRKEEILLRLSPQVNEVNISKESQKKKHSYNSGFVVSTSRLNDSEITIHISCYTVHKIPLSKILMCLENDGLLLLNISSSQTYGGRVFCNSHFQVDKAQRLEFDILNEKLLSIMEKEGGFSIQ*