AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0067430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0067430 MtrunA17_Chr1g0171291 89.753 283 14 1 1 283 1 268 7.29e-163 452
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0067430 AT1G42990.1 37.255 153 78 5 135 283 155 293 2.67e-14 72.0

Find 90 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCTTCTTCGTAATTTC+TGG 0.193725 1_2:+32427367 Msa0067430:five_prime_UTR
GGGTTCCCTGCTATGGTTCC+TGG 0.201475 1_2:+32429388 Msa0067430:CDS
GAGAGATTCGAAATCGATTT+GGG 0.252626 1_2:-32427411 None:intergenic
CTAAGAAGGGTGGCTCTAAA+AGG 0.288472 1_2:+32429494 Msa0067430:CDS
CAAACATGTTGGGATGCTTT+TGG 0.296968 1_2:+32429778 Msa0067430:three_prime_UTR
AGAGAGATTCGAAATCGATT+TGG 0.334822 1_2:-32427412 None:intergenic
TTCTTCCTATTTGAAACATA+TGG 0.336637 1_2:+32429844 Msa0067430:three_prime_UTR
CAAAAGCATCCCAACATGTT+TGG 0.338640 1_2:-32429777 None:intergenic
GAAGGCTCTTGAGGAGTTAC+TGG 0.356888 1_2:-32427674 None:intergenic
GGAGTCTGCTGTGCTCTTGT+TGG 0.367032 1_2:+32428146 Msa0067430:CDS
AACTTCCATATGTTTCAAAT+AGG 0.377154 1_2:-32429849 None:intergenic
GGAACCTCTTGAGGAGTTAC+TGG 0.381189 1_2:-32427719 None:intergenic
TCTTCTTCTTCGTAATTTCT+GGG 0.384078 1_2:+32427368 Msa0067430:five_prime_UTR
TTGTGTTGTCTTGGTGGTTA+AGG 0.394204 1_2:-32427259 None:intergenic
TTGCAATCGCGTGGTGGCTT+TGG 0.414223 1_2:+32428105 Msa0067430:CDS
TGCTGTTGGGTTCCCTGCTA+TGG 0.415075 1_2:+32429381 Msa0067430:CDS
TTTGCAGAATCCCTGCTGTT+GGG 0.426943 1_2:+32429368 Msa0067430:intron
GAATATCTTCGAGAAACTTA+TGG 0.427802 1_2:-32427586 None:intergenic
CCTCGACCCGATCCCTCAAT+CGG 0.430109 1_2:+32427610 Msa0067430:CDS
GGTTCCCTGCTATGGTTCCT+GGG 0.440801 1_2:+32429389 Msa0067430:CDS
AGCCTTCTTCCGATGAAGCC+AGG 0.446524 1_2:+32427690 Msa0067430:CDS
GAGTCTGCTGTGCTCTTGTT+GGG 0.447976 1_2:+32428147 Msa0067430:CDS
TCATCTTCGTTCTTGAAGCT+TGG 0.448828 1_2:-32429580 None:intergenic
TGGATCAACCCTGGCTTCAT+CGG 0.451066 1_2:-32427699 None:intergenic
TGATCATCCGATTGAGGGAT+CGG 0.459847 1_2:-32427617 None:intergenic
TGATGCCCAGGAACCATAGC+AGG 0.462353 1_2:-32429394 None:intergenic
CGTTGATTGTGTTGTCTTGG+TGG 0.465407 1_2:-32427265 None:intergenic
TGGCGTTGATTGTGTTGTCT+TGG 0.484926 1_2:-32427268 None:intergenic
AAAGAAAATGCAAAGAAAGA+AGG 0.485287 1_2:-32429664 None:intergenic
TCTTTACAAACGATTGAGTC+AGG 0.488376 1_2:-32429547 None:intergenic
ATATGTAAACCAAACATGTT+GGG 0.490186 1_2:+32429768 Msa0067430:three_prime_UTR
TGAAGGGGAATGCAGAAGAC+TGG 0.491226 1_2:+32428032 Msa0067430:CDS
TGGAATTGCGTCGTCATCAT+CGG 0.494775 1_2:+32427553 Msa0067430:CDS
AGCAGACTCCTGCATGGTCA+TGG 0.499805 1_2:-32428133 None:intergenic
AATATGTAAACCAAACATGT+TGG 0.501452 1_2:+32429767 Msa0067430:three_prime_UTR
AACCTTGATCATCCGATTGA+GGG 0.507174 1_2:-32427622 None:intergenic
ATGCTGAGAACCATGCTTTG+CGG 0.510527 1_2:+32428076 Msa0067430:CDS
AAACCTTGATCATCCGATTG+AGG 0.512112 1_2:-32427623 None:intergenic
CCGATTGAGGGATCGGGTCG+AGG 0.513783 1_2:-32427610 None:intergenic
CTGTTGTTAGCCACAGTGGA+AGG 0.514860 1_2:-32429436 None:intergenic
TGGAACACAAAGGTCTAAGA+AGG 0.519321 1_2:+32429480 Msa0067430:CDS
TGGTGCTCCCATGACCATGC+AGG 0.523079 1_2:+32428125 Msa0067430:CDS
ACCCTGGCTTCATCGGAAGA+AGG 0.528508 1_2:-32427692 None:intergenic
TTGGGGTTCTAAATTGTCCA+TGG 0.534572 1_2:-32427393 None:intergenic
TCATCGGAAGAAGGCTCTTG+AGG 0.542473 1_2:-32427683 None:intergenic
AGGAGTTACTGGATCAACTC+TGG 0.544068 1_2:-32427663 None:intergenic
TTCGAGAAACTTATGGTAGT+CGG 0.545198 1_2:-32427579 None:intergenic
GTTGTTAGCCACAGTGGAAG+GGG 0.545341 1_2:-32429434 None:intergenic
ATCCCTCAATCGGATGATCA+AGG 0.545906 1_2:+32427620 Msa0067430:CDS
TCAACTCTGGCTTCATCAGA+AGG 0.547608 1_2:-32427650 None:intergenic
ATAGCAGGGAACCCAACAGC+AGG 0.548068 1_2:-32429379 None:intergenic
CTTTGTTTGCAATCGCGTGG+TGG 0.550397 1_2:+32428099 Msa0067430:CDS
TAAGAAGGGTGGCTCTAAAA+GGG 0.555483 1_2:+32429495 Msa0067430:CDS
GCCTTCTTCCGATGAAGCCA+GGG 0.556367 1_2:+32427691 Msa0067430:CDS
TTCGTAATTTCTGGGTACCA+TGG 0.560503 1_2:+32427376 Msa0067430:exon
GCAGACTCCTGCATGGTCAT+GGG 0.561161 1_2:-32428132 None:intergenic
CTTCTCATGTCTGATGCCGA+TGG 0.561993 1_2:+32427533 Msa0067430:CDS
GCAAACAAAGCCGCAAAGCA+TGG 0.573484 1_2:-32428086 None:intergenic
TGAGTTGGCACATGATGCCC+AGG 0.575121 1_2:-32429406 None:intergenic
TCAATCTCTGAGAGAGGCGA+GGG 0.577892 1_2:-32427506 None:intergenic
GATCATCCGATTGAGGGATC+GGG 0.580413 1_2:-32427616 None:intergenic
AAGATGATGAATGATTTGAA+TGG 0.581112 1_2:-32427349 None:intergenic
TGTTGTTAGCCACAGTGGAA+GGG 0.582265 1_2:-32429435 None:intergenic
CTCAATCTCTGAGAGAGGCG+AGG 0.584965 1_2:-32427507 None:intergenic
GGTTCAGGAGGAACCTCTTG+AGG 0.589392 1_2:-32427728 None:intergenic
TGATGACGACGCAATTCCAT+CGG 0.590462 1_2:-32427549 None:intergenic
GTTACCTCCAAGAGAAAACA+TGG 0.596124 1_2:+32429460 Msa0067430:CDS
CTGCTGTGAAGTCAAGAGAG+AGG 0.599823 1_2:+32427965 Msa0067430:CDS
GTGGAAGGGGTAGACTGAGT+TGG 0.606292 1_2:-32429421 None:intergenic
AGGAGTTACTGGATCAACCC+TGG 0.607536 1_2:-32427708 None:intergenic
CGGCTTTGTTTGCAATCGCG+TGG 0.608761 1_2:+32428096 Msa0067430:CDS
GTGTCTGCGACGGAAACAGA+AGG 0.621465 1_2:-32427482 None:intergenic
GAACAAATAGAACATCTGAA+AGG 0.627981 1_2:-32429642 None:intergenic
AACACTGTTGTTAGCCACAG+TGG 0.628137 1_2:-32429440 None:intergenic
AAAATCTTGAGACGAAGAGT+AGG 0.630085 1_2:+32428004 Msa0067430:CDS
AGGCGAGGGAGTGTCTGCGA+CGG 0.634385 1_2:-32427492 None:intergenic
TGATCCAGTAACTCCTCAAG+AGG 0.636811 1_2:+32427715 Msa0067430:CDS
AAAAGTGAGAGAGATAGAGA+GGG 0.638866 1_2:-32429697 None:intergenic
AGATTCTCAATCTCTGAGAG+AGG 0.640144 1_2:-32427512 None:intergenic
AGAGATTCGAAATCGATTTG+GGG 0.646761 1_2:-32427410 None:intergenic
GATTTGAATGGAATTAGTGA+AGG 0.655321 1_2:-32427337 None:intergenic
TTTCAGGCAGATGAGGAACA+GGG 0.660559 1_2:+32427939 Msa0067430:intron
GGAACACAAAGGTCTAAGAA+GGG 0.664648 1_2:+32429481 Msa0067430:CDS
GTGAGAGCGGTTATAAACAG+TGG 0.666996 1_2:-32427288 None:intergenic
TAGCAGGGAACCCAACAGCA+GGG 0.674308 1_2:-32429378 None:intergenic
ACACAAAGGTCTAAGAAGGG+TGG 0.678689 1_2:+32429484 Msa0067430:CDS
GAGCACAGCAGACTCCTGCA+TGG 0.688063 1_2:-32428139 None:intergenic
GATGCCCAGGAACCATAGCA+GGG 0.695327 1_2:-32429393 None:intergenic
AGAGAAAACATGGAACACAA+AGG 0.721160 1_2:+32429470 Msa0067430:CDS
TCAGTCTACCCCTTCCACTG+TGG 0.742334 1_2:+32429426 Msa0067430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTCATTATCATTTTATTTT+AGG + chr1_2:32428221-32428240 Msa0067430:intron 15.0%
!! ATAGTTTCAATTTCATATAT+TGG + chr1_2:32428398-32428417 Msa0067430:intron 15.0%
!!! TTACATATTTTTTTGTAACT+AGG - chr1_2:32429756-32429775 None:intergenic 15.0%
!!! TAATTTGTAATTTGTATTCG+AGG + chr1_2:32427850-32427869 Msa0067430:intron 20.0%
!! GTAAGGAAAAAAAAATTGTT+AGG - chr1_2:32428344-32428363 None:intergenic 20.0%
!! ATACATAAACTCACTAATTA+TGG - chr1_2:32428890-32428909 None:intergenic 20.0%
!! TAAATGGAAAAAAATTGTGT+GGG + chr1_2:32429004-32429023 Msa0067430:intron 20.0%
!! TACATAAAACATAAACAACA+TGG + chr1_2:32429164-32429183 Msa0067430:intron 20.0%
!! ATTCATTTAACATGCATAAA+GGG - chr1_2:32429222-32429241 None:intergenic 20.0%
!! TATTCATTTAACATGCATAA+AGG - chr1_2:32429223-32429242 None:intergenic 20.0%
!!! ATGTTATAAAACTTTTGAAG+TGG + chr1_2:32429617-32429636 Msa0067430:exon 20.0%
!!! TGTTATAAAACTTTTGAAGT+GGG + chr1_2:32429618-32429637 Msa0067430:exon 20.0%
! AAAAGAAAAAAAAGTGAGAG+CGG - chr1_2:32427304-32427323 None:intergenic 25.0%
! AAGATGATGAATGATTTGAA+TGG - chr1_2:32427352-32427371 None:intergenic 25.0%
!!! CTAATTAGCTTTTTTGAAAC+GGG - chr1_2:32427752-32427771 None:intergenic 25.0%
!!! CCTAATTAGCTTTTTTGAAA+CGG - chr1_2:32427753-32427772 None:intergenic 25.0%
!!! TTTTTTTTTTTTCAACCTGC+TGG + chr1_2:32427825-32427844 Msa0067430:intron 25.0%
!! CTAAAAGTATAACCATATGA+AGG + chr1_2:32428260-32428279 Msa0067430:intron 25.0%
!! TTTTTCCTTACTTTCTTGAA+TGG + chr1_2:32428353-32428372 Msa0067430:intron 25.0%
! AACTATATAGCATTAGCAAA+AGG - chr1_2:32428384-32428403 None:intergenic 25.0%
! AGAGATTAAGGTTACAATTT+GGG - chr1_2:32428438-32428457 None:intergenic 25.0%
! TAGAGATTAAGGTTACAATT+TGG - chr1_2:32428439-32428458 None:intergenic 25.0%
!!! ACGTCTTTAGGTTTTTTATT+GGG + chr1_2:32428524-32428543 Msa0067430:intron 25.0%
! TTTAACAGTGTTATGAATAG+TGG + chr1_2:32428586-32428605 Msa0067430:intron 25.0%
!! AAACAGAAAACCTTAGATTT+TGG + chr1_2:32428759-32428778 Msa0067430:intron 25.0%
! CTAAATGGAAAAAAATTGTG+TGG + chr1_2:32429003-32429022 Msa0067430:intron 25.0%
!!! TCAGAGATATTTTTACTGTT+TGG - chr1_2:32429521-32429540 None:intergenic 25.0%
!!! GTTATAAAACTTTTGAAGTG+GGG + chr1_2:32429619-32429638 Msa0067430:exon 25.0%
! AAAGAAAATGCAAAGAAAGA+AGG - chr1_2:32429667-32429686 None:intergenic 25.0%
! AATATGTAAACCAAACATGT+TGG + chr1_2:32429767-32429786 Msa0067430:three_prime_UTR 25.0%
! ATATGTAAACCAAACATGTT+GGG + chr1_2:32429768-32429787 Msa0067430:three_prime_UTR 25.0%
!! TTCTTCCTATTTGAAACATA+TGG + chr1_2:32429844-32429863 Msa0067430:three_prime_UTR 25.0%
! AACTTCCATATGTTTCAAAT+AGG - chr1_2:32429852-32429871 None:intergenic 25.0%
GATTTGAATGGAATTAGTGA+AGG - chr1_2:32427340-32427359 None:intergenic 30.0%
ATCTTCTTCTTCGTAATTTC+TGG + chr1_2:32427367-32427386 Msa0067430:five_prime_UTR 30.0%
TCTTCTTCTTCGTAATTTCT+GGG + chr1_2:32427368-32427387 Msa0067430:five_prime_UTR 30.0%
GAATATCTTCGAGAAACTTA+TGG - chr1_2:32427589-32427608 None:intergenic 30.0%
CCGTTTCAAAAAAGCTAATT+AGG + chr1_2:32427750-32427769 Msa0067430:CDS 30.0%
AAATTACAAATTACTCCAGC+AGG - chr1_2:32427843-32427862 None:intergenic 30.0%
!!! TGTTTTGGTATCTGATTTTC+AGG + chr1_2:32427923-32427942 Msa0067430:intron 30.0%
GGTTTCATTATTGCATTCTT+TGG + chr1_2:32428310-32428329 Msa0067430:intron 30.0%
TATTGCATTCTTTGGTTGTT+TGG + chr1_2:32428318-32428337 Msa0067430:intron 30.0%
ACAGTAGTCATTAGAGATTA+AGG - chr1_2:32428450-32428469 None:intergenic 30.0%
ATAACAGCAAGAAGAAAACA+TGG - chr1_2:32428486-32428505 None:intergenic 30.0%
!!! GACGTCTTTAGGTTTTTTAT+TGG + chr1_2:32428523-32428542 Msa0067430:intron 30.0%
!! TCTAAGGTTTTCTGTTTCAT+GGG - chr1_2:32428756-32428775 None:intergenic 30.0%
!! ATCTAAGGTTTTCTGTTTCA+TGG - chr1_2:32428757-32428776 None:intergenic 30.0%
CTAAGATACTCCAAAATCTA+AGG - chr1_2:32428772-32428791 None:intergenic 30.0%
!!! CTTAGATTTTGGAGTATCTT+AGG + chr1_2:32428770-32428789 Msa0067430:intron 30.0%
ATAATATTTCACCTTCTCAC+TGG + chr1_2:32428857-32428876 Msa0067430:intron 30.0%
TAGTGAGTTTATGTATGTAC+TGG + chr1_2:32428894-32428913 Msa0067430:intron 30.0%
!! TTTAGGTTTTTGCGTCTAAA+TGG + chr1_2:32428988-32429007 Msa0067430:intron 30.0%
ACCTTTGGACCAAAAAAAAA+AGG - chr1_2:32429100-32429119 None:intergenic 30.0%
TTTGCATAAGTACACAAATC+TGG - chr1_2:32429143-32429162 None:intergenic 30.0%
!!! TACTTTTTCTCTGAGAGTTT+TGG - chr1_2:32429195-32429214 None:intergenic 30.0%
! GTTTTATGAAGTTGCTCTAA+TGG + chr1_2:32429294-32429313 Msa0067430:intron 30.0%
GAACAAATAGAACATCTGAA+AGG - chr1_2:32429645-32429664 None:intergenic 30.0%
!! AGAGATTCGAAATCGATTTG+GGG - chr1_2:32427413-32427432 None:intergenic 35.0%
! GAGAGATTCGAAATCGATTT+GGG - chr1_2:32427414-32427433 None:intergenic 35.0%
AGAGAGATTCGAAATCGATT+TGG - chr1_2:32427415-32427434 None:intergenic 35.0%
ATCATCCTCCTGCAAAAAAT+CGG - chr1_2:32427456-32427475 None:intergenic 35.0%
TTCGAGAAACTTATGGTAGT+CGG - chr1_2:32427582-32427601 None:intergenic 35.0%
!!! GGTTCTAATGAGAAGTGTTT+TGG + chr1_2:32427908-32427927 Msa0067430:intron 35.0%
AAAATCTTGAGACGAAGAGT+AGG + chr1_2:32428004-32428023 Msa0067430:CDS 35.0%
!! ACGAAGAGTAGGTATTTTGA+AGG + chr1_2:32428015-32428034 Msa0067430:CDS 35.0%
!! CGAAGAGTAGGTATTTTGAA+GGG + chr1_2:32428016-32428035 Msa0067430:CDS 35.0%
!! GAAGAGTAGGTATTTTGAAG+GGG + chr1_2:32428017-32428036 Msa0067430:CDS 35.0%
TCCTTACTTTCTTGAATGGA+TGG + chr1_2:32428357-32428376 Msa0067430:intron 35.0%
TCCATCCATTCAAGAAAGTA+AGG - chr1_2:32428361-32428380 None:intergenic 35.0%
CCTAAAGACGTCTTTAACAT+CGG - chr1_2:32428515-32428534 None:intergenic 35.0%
TGTTATGAATAGTGGTGTAG+CGG + chr1_2:32428594-32428613 Msa0067430:intron 35.0%
AAAAACACCATGACAGTGTT+CGG + chr1_2:32428808-32428827 Msa0067430:intron 35.0%
TGACCTCAAAAACACTATAG+CGG - chr1_2:32428925-32428944 None:intergenic 35.0%
AGCAACTATGCTATGTTATC+TGG + chr1_2:32428944-32428963 Msa0067430:intron 35.0%
GCAACTATGCTATGTTATCT+GGG + chr1_2:32428945-32428964 Msa0067430:intron 35.0%
TAACAATCACAACCACTTGT+TGG + chr1_2:32429042-32429061 Msa0067430:intron 35.0%
CAAAAAAAAAAGGCTACAGG+AGG - chr1_2:32429090-32429109 None:intergenic 35.0%
!!! CTCCTGTAGCCTTTTTTTTT+TGG + chr1_2:32429088-32429107 Msa0067430:intron 35.0%
GACCAAAAAAAAAAGGCTAC+AGG - chr1_2:32429093-32429112 None:intergenic 35.0%
!!! GCCTTTTTTTTTTGGTCCAA+AGG + chr1_2:32429096-32429115 Msa0067430:intron 35.0%
CAAATCTGGATTACAGCAAA+AGG - chr1_2:32429129-32429148 None:intergenic 35.0%
! TTGTGTTCCATGTTTTCTCT+TGG - chr1_2:32429470-32429489 None:intergenic 35.0%
AGAGAAAACATGGAACACAA+AGG + chr1_2:32429470-32429489 Msa0067430:CDS 35.0%
TCTTTACAAACGATTGAGTC+AGG - chr1_2:32429550-32429569 None:intergenic 35.0%
AAAAGTGAGAGAGATAGAGA+GGG - chr1_2:32429700-32429719 None:intergenic 35.0%
AAAAAGTGAGAGAGATAGAG+AGG - chr1_2:32429701-32429720 None:intergenic 35.0%
!! TCTCTATCTCTCTCACTTTT+TGG + chr1_2:32429700-32429719 Msa0067430:three_prime_UTR 35.0%
! TTGTGTTGTCTTGGTGGTTA+AGG - chr1_2:32427262-32427281 None:intergenic 40.0%
! TTCGTAATTTCTGGGTACCA+TGG + chr1_2:32427376-32427395 Msa0067430:exon 40.0%
!! TTGGGGTTCTAAATTGTCCA+TGG - chr1_2:32427396-32427415 None:intergenic 40.0%
! AGATTCTCAATCTCTGAGAG+AGG - chr1_2:32427515-32427534 None:intergenic 40.0%
AACCTTGATCATCCGATTGA+GGG - chr1_2:32427625-32427644 None:intergenic 40.0%
AAACCTTGATCATCCGATTG+AGG - chr1_2:32427626-32427645 None:intergenic 40.0%
!!! TTTTTTGAAACGGGTTCAGG+AGG - chr1_2:32427743-32427762 None:intergenic 40.0%
!!! AGCTTTTTTGAAACGGGTTC+AGG - chr1_2:32427746-32427765 None:intergenic 40.0%
TTCGAGGTTACCGAATTTGA+TGG + chr1_2:32427866-32427885 Msa0067430:intron 40.0%
TCGAGGTTACCGAATTTGAT+GGG + chr1_2:32427867-32427886 Msa0067430:intron 40.0%
CCATAAGACCCCATCAAATT+CGG - chr1_2:32427879-32427898 None:intergenic 40.0%
! CGAATTTGATGGGGTCTTAT+GGG + chr1_2:32427877-32427896 Msa0067430:intron 40.0%
!! ATCTGATTTTCAGGCAGATG+AGG + chr1_2:32427932-32427951 Msa0067430:intron 40.0%
GGTCACTGTAATCCTTCATA+TGG - chr1_2:32428275-32428294 None:intergenic 40.0%
GAAACCAAATCATTCGTGCA+AGG - chr1_2:32428296-32428315 None:intergenic 40.0%
CCGATGTTAAAGACGTCTTT+AGG + chr1_2:32428512-32428531 Msa0067430:intron 40.0%
ATTTCAGATCTCATCTCGTG+CGG - chr1_2:32428704-32428723 None:intergenic 40.0%
AAAACACCATGACAGTGTTC+GGG + chr1_2:32428809-32428828 Msa0067430:intron 40.0%
!! ATCCCGCTATAGTGTTTTTG+AGG + chr1_2:32428919-32428938 Msa0067430:intron 40.0%
GACCTCAAAAACACTATAGC+GGG - chr1_2:32428924-32428943 None:intergenic 40.0%
! GATACATACCACTGCTGTTT+AGG + chr1_2:32428971-32428990 Msa0067430:intron 40.0%
ATCTGGATTACAGCAAAAGG+AGG - chr1_2:32429126-32429145 None:intergenic 40.0%
TCTTCGAATGATGGCAACAT+TGG - chr1_2:32429270-32429289 None:intergenic 40.0%
AAAACCCACTCTTCGAATGA+TGG - chr1_2:32429279-32429298 None:intergenic 40.0%
GTTACCTCCAAGAGAAAACA+TGG + chr1_2:32429460-32429479 Msa0067430:CDS 40.0%
! TGTTCCATGTTTTCTCTTGG+AGG - chr1_2:32429467-32429486 None:intergenic 40.0%
TGGAACACAAAGGTCTAAGA+AGG + chr1_2:32429480-32429499 Msa0067430:CDS 40.0%
GGAACACAAAGGTCTAAGAA+GGG + chr1_2:32429481-32429500 Msa0067430:CDS 40.0%
!! TAAGAAGGGTGGCTCTAAAA+GGG + chr1_2:32429495-32429514 Msa0067430:CDS 40.0%
TCATCTTCGTTCTTGAAGCT+TGG - chr1_2:32429583-32429602 None:intergenic 40.0%
!! CAAAAGCATCCCAACATGTT+TGG - chr1_2:32429780-32429799 None:intergenic 40.0%
! CAAACATGTTGGGATGCTTT+TGG + chr1_2:32429778-32429797 Msa0067430:three_prime_UTR 40.0%
! CGTTGATTGTGTTGTCTTGG+TGG - chr1_2:32427268-32427287 None:intergenic 45.0%
!! TGGCGTTGATTGTGTTGTCT+TGG - chr1_2:32427271-32427290 None:intergenic 45.0%
GTGAGAGCGGTTATAAACAG+TGG - chr1_2:32427291-32427310 None:intergenic 45.0%
! TGATGACGACGCAATTCCAT+CGG - chr1_2:32427552-32427571 None:intergenic 45.0%
TGGAATTGCGTCGTCATCAT+CGG + chr1_2:32427553-32427572 Msa0067430:CDS 45.0%
TGATCATCCGATTGAGGGAT+CGG - chr1_2:32427620-32427639 None:intergenic 45.0%
ATCCCTCAATCGGATGATCA+AGG + chr1_2:32427620-32427639 Msa0067430:CDS 45.0%
! TCAACTCTGGCTTCATCAGA+AGG - chr1_2:32427653-32427672 None:intergenic 45.0%
AGGAGTTACTGGATCAACTC+TGG - chr1_2:32427666-32427685 None:intergenic 45.0%
TGATCCAGTAACTCCTCAAG+AGG + chr1_2:32427670-32427689 Msa0067430:CDS 45.0%
CGAGGTTACCGAATTTGATG+GGG + chr1_2:32427868-32427887 Msa0067430:intron 45.0%
CCGAATTTGATGGGGTCTTA+TGG + chr1_2:32427876-32427895 Msa0067430:intron 45.0%
! GGGGTCTTATGGGATGATTA+TGG + chr1_2:32427887-32427906 Msa0067430:intron 45.0%
!! TTTTCAGGCAGATGAGGAAC+AGG + chr1_2:32427938-32427957 Msa0067430:intron 45.0%
TTTCAGGCAGATGAGGAACA+GGG + chr1_2:32427939-32427958 Msa0067430:intron 45.0%
ATGCTGAGAACCATGCTTTG+CGG + chr1_2:32428076-32428095 Msa0067430:CDS 45.0%
GTGACCTTGCACGAATGATT+TGG + chr1_2:32428289-32428308 Msa0067430:intron 45.0%
! AGCTTGTTTGTAGCAGCCAA+GGG - chr1_2:32428625-32428644 None:intergenic 45.0%
GGCCAATATAGCTCATGTTC+CGG + chr1_2:32428667-32428686 Msa0067430:intron 45.0%
CTTCTCATGCTGTCTCATTC+AGG + chr1_2:32428832-32428851 Msa0067430:intron 45.0%
CGCAAAAACCTAAACAGCAG+TGG - chr1_2:32428982-32429001 None:intergenic 45.0%
CTCTGACAAAGACCAACAAG+TGG - chr1_2:32429057-32429076 None:intergenic 45.0%
! AGCAAAAGGAGGTACACCTT+TGG - chr1_2:32429115-32429134 None:intergenic 45.0%
TGTTGCCATCATTCGAAGAG+TGG + chr1_2:32429271-32429290 Msa0067430:intron 45.0%
GTTGCCATCATTCGAAGAGT+GGG + chr1_2:32429272-32429291 Msa0067430:intron 45.0%
! TTTTGCAGAATCCCTGCTGT+TGG + chr1_2:32429367-32429386 Msa0067430:intron 45.0%
TTTGCAGAATCCCTGCTGTT+GGG + chr1_2:32429368-32429387 Msa0067430:intron 45.0%
TGTTGTTAGCCACAGTGGAA+GGG - chr1_2:32429438-32429457 None:intergenic 45.0%
AACACTGTTGTTAGCCACAG+TGG - chr1_2:32429443-32429462 None:intergenic 45.0%
ACACAAAGGTCTAAGAAGGG+TGG + chr1_2:32429484-32429503 Msa0067430:CDS 45.0%
!! CTAAGAAGGGTGGCTCTAAA+AGG + chr1_2:32429494-32429513 Msa0067430:CDS 45.0%
!! AAAAGTAAAATAAATAAAAA+AGG - chr1_2:32427239-32427258 None:intergenic 5.0%
!!! CGAAGTCGCCGATTTTTTGC+AGG + chr1_2:32427445-32427464 Msa0067430:CDS 50.0%
!!! AGTCGCCGATTTTTTGCAGG+AGG + chr1_2:32427448-32427467 Msa0067430:CDS 50.0%
! TCAATCTCTGAGAGAGGCGA+GGG - chr1_2:32427509-32427528 None:intergenic 50.0%
CTTCTCATGTCTGATGCCGA+TGG + chr1_2:32427533-32427552 Msa0067430:CDS 50.0%
GATCATCCGATTGAGGGATC+GGG - chr1_2:32427619-32427638 None:intergenic 50.0%
GAAGGCTCTTGAGGAGTTAC+TGG - chr1_2:32427677-32427696 None:intergenic 50.0%
TCATCGGAAGAAGGCTCTTG+AGG - chr1_2:32427686-32427705 None:intergenic 50.0%
TGGATCAACCCTGGCTTCAT+CGG - chr1_2:32427702-32427721 None:intergenic 50.0%
AGGAGTTACTGGATCAACCC+TGG - chr1_2:32427711-32427730 None:intergenic 50.0%
GGAACCTCTTGAGGAGTTAC+TGG - chr1_2:32427722-32427741 None:intergenic 50.0%
CTGCTGTGAAGTCAAGAGAG+AGG + chr1_2:32427965-32427984 Msa0067430:CDS 50.0%
TGAAGGGGAATGCAGAAGAC+TGG + chr1_2:32428032-32428051 Msa0067430:CDS 50.0%
GCAAACAAAGCCGCAAAGCA+TGG - chr1_2:32428089-32428108 None:intergenic 50.0%
! CTTTGTTTGCAATCGCGTGG+TGG + chr1_2:32428099-32428118 Msa0067430:CDS 50.0%
GAGTCTGCTGTGCTCTTGTT+GGG + chr1_2:32428147-32428166 Msa0067430:CDS 50.0%
TGCTGTGCTCTTGTTGGGTA+AGG + chr1_2:32428152-32428171 Msa0067430:intron 50.0%
! TGAATAGTGGTGTAGCGGAG+TGG + chr1_2:32428599-32428618 Msa0067430:intron 50.0%
! CAGCTTGTTTGTAGCAGCCA+AGG - chr1_2:32428626-32428645 None:intergenic 50.0%
GGCCGGAACATGAGCTATAT+TGG - chr1_2:32428672-32428691 None:intergenic 50.0%
ATGGTGACTGACCAGTGAGA+AGG - chr1_2:32428871-32428890 None:intergenic 50.0%
GTTGTTAGCCACAGTGGAAG+GGG - chr1_2:32429437-32429456 None:intergenic 50.0%
CTGTTGTTAGCCACAGTGGA+AGG - chr1_2:32429439-32429458 None:intergenic 50.0%
GTGTCTGCGACGGAAACAGA+AGG - chr1_2:32427485-32427504 None:intergenic 55.0%
! CTCAATCTCTGAGAGAGGCG+AGG - chr1_2:32427510-32427529 None:intergenic 55.0%
AGCCTTCTTCCGATGAAGCC+AGG + chr1_2:32427690-32427709 Msa0067430:CDS 55.0%
GCCTTCTTCCGATGAAGCCA+GGG + chr1_2:32427691-32427710 Msa0067430:CDS 55.0%
ACCCTGGCTTCATCGGAAGA+AGG - chr1_2:32427695-32427714 None:intergenic 55.0%
GGTTCAGGAGGAACCTCTTG+AGG - chr1_2:32427731-32427750 None:intergenic 55.0%
!! CGGCTTTGTTTGCAATCGCG+TGG + chr1_2:32428096-32428115 Msa0067430:CDS 55.0%
TTGCAATCGCGTGGTGGCTT+TGG + chr1_2:32428105-32428124 Msa0067430:CDS 55.0%
GCAGACTCCTGCATGGTCAT+GGG - chr1_2:32428135-32428154 None:intergenic 55.0%
AGCAGACTCCTGCATGGTCA+TGG - chr1_2:32428136-32428155 None:intergenic 55.0%
GGAGTCTGCTGTGCTCTTGT+TGG + chr1_2:32428146-32428165 Msa0067430:CDS 55.0%
TAGTGCAGCTGCTGCTAGAG+CGG + chr1_2:32428646-32428665 Msa0067430:intron 55.0%
CATCTCGTGCGGCACTTTAG+AGG - chr1_2:32428693-32428712 None:intergenic 55.0%
GAGAAGCCCGAACACTGTCA+TGG - chr1_2:32428818-32428837 None:intergenic 55.0%
TAGCAGGGAACCCAACAGCA+GGG - chr1_2:32429381-32429400 None:intergenic 55.0%
ATAGCAGGGAACCCAACAGC+AGG - chr1_2:32429382-32429401 None:intergenic 55.0%
TGCTGTTGGGTTCCCTGCTA+TGG + chr1_2:32429381-32429400 Msa0067430:CDS 55.0%
GGTTCCCTGCTATGGTTCCT+GGG + chr1_2:32429389-32429408 Msa0067430:CDS 55.0%
GATGCCCAGGAACCATAGCA+GGG - chr1_2:32429396-32429415 None:intergenic 55.0%
TGATGCCCAGGAACCATAGC+AGG - chr1_2:32429397-32429416 None:intergenic 55.0%
! TGAGTTGGCACATGATGCCC+AGG - chr1_2:32429409-32429428 None:intergenic 55.0%
GTGGAAGGGGTAGACTGAGT+TGG - chr1_2:32429424-32429443 None:intergenic 55.0%
TCAGTCTACCCCTTCCACTG+TGG + chr1_2:32429426-32429445 Msa0067430:CDS 55.0%
CCTCGACCCGATCCCTCAAT+CGG + chr1_2:32427610-32427629 Msa0067430:CDS 60.0%
TGGTGCTCCCATGACCATGC+AGG + chr1_2:32428125-32428144 Msa0067430:CDS 60.0%
GAGCACAGCAGACTCCTGCA+TGG - chr1_2:32428142-32428161 None:intergenic 60.0%
! TCGTGCGGCACTTTAGAGGC+CGG - chr1_2:32428689-32428708 None:intergenic 60.0%
GGGTTCCCTGCTATGGTTCC+TGG + chr1_2:32429388-32429407 Msa0067430:CDS 60.0%
!! AGGCGAGGGAGTGTCTGCGA+CGG - chr1_2:32427495-32427514 None:intergenic 65.0%
CCGATTGAGGGATCGGGTCG+AGG - chr1_2:32427613-32427632 None:intergenic 65.0%
! TGGTGTAGCGGAGTGGCCCT+TGG + chr1_2:32428606-32428625 Msa0067430:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 32427233 32429913 32427233 ID=Msa0067430;Name=Msa0067430
chr1_2 mRNA 32427233 32429913 32427233 ID=Msa0067430-mRNA-1;Parent=Msa0067430;Name=Msa0067430-mRNA-1;_AED=0.03;_eAED=0.03;_QI=162|1|1|1|0.5|1|3|288|283
chr1_2 exon 32427233 32427771 32427233 ID=Msa0067430-mRNA-1:exon:10605;Parent=Msa0067430-mRNA-1
chr1_2 exon 32427945 32428168 32427945 ID=Msa0067430-mRNA-1:exon:10606;Parent=Msa0067430-mRNA-1
chr1_2 exon 32429375 32429913 32429375 ID=Msa0067430-mRNA-1:exon:10607;Parent=Msa0067430-mRNA-1
chr1_2 five_prime_UTR 32427233 32427394 32427233 ID=Msa0067430-mRNA-1:five_prime_utr;Parent=Msa0067430-mRNA-1
chr1_2 CDS 32427395 32427771 32427395 ID=Msa0067430-mRNA-1:cds;Parent=Msa0067430-mRNA-1
chr1_2 CDS 32427945 32428168 32427945 ID=Msa0067430-mRNA-1:cds;Parent=Msa0067430-mRNA-1
chr1_2 CDS 32429375 32429625 32429375 ID=Msa0067430-mRNA-1:cds;Parent=Msa0067430-mRNA-1
chr1_2 three_prime_UTR 32429626 32429913 32429626 ID=Msa0067430-mRNA-1:three_prime_utr;Parent=Msa0067430-mRNA-1
Gene Sequence

>Msa0067430

ATGGACAATTTAGAACCCCAAATCGATTTCGAATCTCTCTTCGCAGAATACGAAGTCGCCGATTTTTTGCAGGAGGATGATATCAATCCTTCTGTTTCCGTCGCAGACACTCCCTCGCCTCTCTCAGAGATTGAGAATCTTCTCATGTCTGATGCCGATGGAATTGCGTCGTCATCATCGGATTCCGACTACCATAAGTTTCTCGAAGATATTCTCCTCGACCCGATCCCTCAATCGGATGATCAAGGTTTCGTTCCTTCTGATGAAGCCAGAGTTGATCCAGTAACTCCTCAAGAGCCTTCTTCCGATGAAGCCAGGGTTGATCCAGTAACTCCTCAAGAGGTTCCTCCTGAACCCGTTTCAAAAAAGCTAATTAGGCAGATGAGGAACAGGGATGCTGCTGTGAAGTCAAGAGAGAGGAAGAAGATGTACGTGAAAAATCTTGAGACGAAGAGTAGGTATTTTGAAGGGGAATGCAGAAGACTGGAGCATTTGCTTCAGTGCTGCTATGCTGAGAACCATGCTTTGCGGCTTTGTTTGCAATCGCGTGGTGGCTTTGGTGCTCCCATGACCATGCAGGAGTCTGCTGTGCTCTTGTTGGAATCCCTGCTGTTGGGTTCCCTGCTATGGTTCCTGGGCATCATGTGCCAACTCAGTCTACCCCTTCCACTGTGGCTAACAACAGTGTTACCTCCAAGAGAAAACATGGAACACAAAGGTCTAAGAAGGGTGGCTCTAAAAGGGCCAAACAGTAAAAATATCTCTGATTATTTCCTGACTCAATCGTTTGTAAAGAGTAGAAGATGCCAAGCTTCAAGAACGAAGATGAAATTTGATTTTATCATGTTATAA
Protein sequence

>Msa0067430

MDNLEPQIDFESLFAEYEVADFLQEDDINPSVSVADTPSPLSEIENLLMSDADGIASSSSDSDYHKFLEDILLDPIPQSDDQGFVPSDEARVDPVTPQEPSSDEARVDPVTPQEVPPEPVSKKLIRQMRNRDAAVKSRERKKMYVKNLETKSRYFEGECRRLEHLLQCCYAENHALRLCLQSRGGFGAPMTMQESAVLLLESLLLGSLLWFLGIMCQLSLPLPLWLTTVLPPRENMEHKGLRRVALKGPNSKNISDYFLTQSFVKSRRCQASRTKMKFDFIML*