AlfalfaGEDB | Msa0067950

M. sativa cultivar zhongmu-4 / Msa0067950

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009909 GO:0010048 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0065007 GO:0070013 GO:0097159 GO:1901363 GO:2000026 GO:2000241
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0067950	KEH26464.1	69.133	473	92	6	8	435	7	470	0.0	634

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0067950	sp\|Q8L3W1\|VRN1_ARATH	24.551	334	215	12	19	321	6	333	1.43e-18	89.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0067950	A0A072U9K2	69.133	473	92	6	8	435	7	470	0.0	634

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TFs/TRs:

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Gene ID	Type	Classification
Msa0067950	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0067950	MtrunA17_Chr6g0476171	76.016	246	54	3	193	435	2	245	7.36e-131	377
Msa0067950	MtrunA17_Chr1g0163141	91.975	162	13	0	31	192	7	168	1.63e-99	295
Msa0067950	MtrunA17_Chr6g0476181	79.245	159	26	1	34	192	1	152	4.88e-83	252
Msa0067950	MtrunA17_Chr1g0163131	45.401	337	139	11	1	323	1	306	3.13e-81	253
Msa0067950	MtrunA17_Chr1g0163131	34.615	130	82	2	306	435	180	306	9.29e-15	74.7
Msa0067950	MtrunA17_Chr1g0163101	63.830	188	45	3	5	192	5	169	1.31e-76	236
Msa0067950	MtrunA17_Chr1g0163181	32.273	440	242	11	28	435	14	429	1.17e-66	219
Msa0067950	MtrunA17_Chr1g0162161	65.409	159	36	2	34	192	3	142	3.36e-65	206
Msa0067950	MtrunA17_Chr1g0163201	34.129	419	213	12	35	435	19	392	1.80e-63	210
Msa0067950	MtrunA17_Chr1g0163261	38.700	323	155	10	19	335	9	294	8.73e-57	189

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0067950	AT3G18990.1	24.551	334	215	12	19	321	6	333	1.46e-19	89.7
Msa0067950	AT3G18990.2	24.551	334	215	12	19	321	6	333	2.40e-19	89.7
Msa0067950	AT4G33280.1	27.640	322	195	17	18	323	19	318	1.49e-14	75.1

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Find 75 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGAATTGGGTGCAACG+CGG	0.811094	1_2:-33280511	Msa0067950:CDS
AAACTAAAAGATCTCCACAT+CGG	0.700040	1_2:+33280443	Msa0067940:intron
AACAATTAGAAGTGTTGGTG+AGG	0.601806	1_2:-33279262	Msa0067950:CDS
AACCACAAAGGAACCAGGCC+CGG	0.431169	1_2:-33279073	Msa0067950:CDS
AAGAAGCATGGTATATTTAG+TGG	0.374789	1_2:-33277226	Msa0067950:CDS
AAGGAACATGAGTTGAAAGT+TGG	0.478819	1_2:-33279159	Msa0067950:CDS
AAGGCAACAATTAGAAGTGT+TGG	0.542696	1_2:-33279267	Msa0067950:CDS
AATCTGACCCTCCTTTGTAT+TGG	0.385935	1_2:+33280419	Msa0067940:intron
AATGGAAACACAGGAGGCAT+GGG	0.555197	1_2:-33279831	Msa0067950:CDS
AATTCAGTGGAGTTGAGAGT+AGG	0.518002	1_2:-33277202	Msa0067950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTCTATTGGACTTAAGATG+TGG	-	chr1_2:33277404-33277423	Msa0067950:intron	30.0%
TAAAAACAAACAACCCTGTA+CGG	-	chr1_2:33277518-33277537	Msa0067950:intron	30.0%
AGCATGATCAAAACAGTATT+GGG	+	chr1_2:33277654-33277673	Msa0067940:intron	30.0%
AAGCATGATCAAAACAGTAT+TGG	+	chr1_2:33277655-33277674	Msa0067940:intron	30.0%
AGTTTAAGGGATATTGGATT+TGG	+	chr1_2:33277917-33277936	Msa0067940:intron	30.0%
TTTGGAAGTTTAAGGGATAT+TGG	+	chr1_2:33277923-33277942	Msa0067940:intron	30.0%
ATATCCCTTAAACTTCCAAA+TGG	-	chr1_2:33277923-33277942	Msa0067950:intron	30.0%
TATCCCTTAAACTTCCAAAT+GGG	-	chr1_2:33277924-33277943	Msa0067950:intron	30.0%
AAACTAAAAGATCTCCACAT+CGG	+	chr1_2:33278027-33278046	Msa0067940:intron	30.0%
AAAAAGAGAACCAACTCAAA+TGG	-	chr1_2:33278241-33278260	Msa0067950:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	33277033	33281456	33277033	ID=Msa0067950;Name=Msa0067950
chr1_2	mRNA	33277033	33281456	33277033	ID=Msa0067950-mRNA-1;Parent=Msa0067950;Name=Msa0067950-mRNA-1;_AED=0.15;_eAED=0.16;_QI=0\|0\|0\|1\|1\|1\|6\|0\|435
chr1_2	exon	33281358	33281456	33281358	ID=Msa0067950-mRNA-1:exon:10988;Parent=Msa0067950-mRNA-1
chr1_2	exon	33280140	33280617	33280140	ID=Msa0067950-mRNA-1:exon:10987;Parent=Msa0067950-mRNA-1
chr1_2	exon	33279710	33279858	33279710	ID=Msa0067950-mRNA-1:exon:10986;Parent=Msa0067950-mRNA-1
chr1_2	exon	33279061	33279340	33279061	ID=Msa0067950-mRNA-1:exon:10985;Parent=Msa0067950-mRNA-1
chr1_2	exon	33277359	33277417	33277359	ID=Msa0067950-mRNA-1:exon:10984;Parent=Msa0067950-mRNA-1
chr1_2	exon	33277033	33277275	33277033	ID=Msa0067950-mRNA-1:exon:10983;Parent=Msa0067950-mRNA-1
chr1_2	CDS	33281358	33281456	33281358	ID=Msa0067950-mRNA-1:cds;Parent=Msa0067950-mRNA-1
chr1_2	CDS	33280140	33280617	33280140	ID=Msa0067950-mRNA-1:cds;Parent=Msa0067950-mRNA-1
chr1_2	CDS	33279710	33279858	33279710	ID=Msa0067950-mRNA-1:cds;Parent=Msa0067950-mRNA-1
chr1_2	CDS	33279061	33279340	33279061	ID=Msa0067950-mRNA-1:cds;Parent=Msa0067950-mRNA-1
chr1_2	CDS	33277359	33277417	33277359	ID=Msa0067950-mRNA-1:cds;Parent=Msa0067950-mRNA-1
chr1_2	CDS	33277033	33277275	33277033	ID=Msa0067950-mRNA-1:cds;Parent=Msa0067950-mRNA-1

Gene Sequence

>Msa0067950

ATGTGGTTTGAACAACACGGCAATGAGCGTGGTAGTGAAGCTAAAGGAATGCATTTCTATTTACATGTTCAACAATGCGAGCTTCAACACAAAGAACTCATGATTCCAAGGAATTTCGTAGAGAGGTATTGGAAAGATGTACCAAATCCAATATCCCTTAAACTTCCAAATGGGTCAGCATGCAAAATGAATTGGGTGCAACGCGGTGATGGTATTTGGCTTCAGAATTGGAAGAGGTTTGCATGGTCGCTCCGATGTGGAGATCTTTTAGTTTTCCAATACAAAGGAGGGTCAGATTTTCAAGTCATTATATTAGATGATAGTAAATTAGAAATTGACTATTCAAGTATGAGATGCAACAACGATCAAGAAAATACTAAAGAGAGTGATGATGATGACGACGACGACGATGAATATGAGTGTGTTGAGATTCCTAGTGACACTGAAAATACAAAAATTCCCCTCAAGAAAAAGAGAACCAACTCAAATGGCATTGCTACTACTGCTGCTACACCTCAAGGGACTAACATTCACAAGAGAAAAACTAATATCAATGCAACCCAGCAAAAAGTTTCGGACGCTAATGGAAACACAGGAGGCATGGGTAAGAATGCTAAAAATGCACAAAAACTAAATGAGAGAAGTTGCACAAATTCTCATCCCAATGAGAGTCCTAGTTTCACTGTCAAATTATCGCCATATCATGTTGAAGGTGATAATAGATTGCCGATACCTAGTTGGTTTTCGAAAGAATACATGAATGAGTTATTGCAAGGGAAGGCAACAATTAGAAGTGTTGGTGAGGACAGTGCATGGCACGTGACACTAAACTTTCAAAAAAGCAGGAGCAGTTTTGTAATGAGGGCTGGTTGGAAGTCATTTTCAAAGGAACATGAGTTGAAAGTTGGTGATGTATGTAAATTTGAGATGACTCAACGTGAACCACTTTCATTCACAATTACTATTATCCCAACCACAAAGGAACCAGGCCCGGAGAAGTTTCAAGATTCTGTAAGTCTGAATTTACATGAGATGTTCAAGGTTTTTATAACATCATCAGACAAGTTGGTTGTACTACCAAGTGAGCTGTTGAAGAAGCATGGTATATTTAGTGGAAATTCAGTGGAGTTGAGAGTAGGGGAAGGAACATGGTTTGTAGATGTGAGTTATAATGAACATCTCGACTATGGTTGGTTCACTAAAGGTTGGCAAGAATTTGTGAGAGATTGCAAAGTAAAGACTGGAGACACTTGCTTGTTTGAGACGATTGATGTGCAAAATCATGTGTTCAAGGTTTCAATTGTTTGA

Protein sequence

>Msa0067950

MWFEQHGNERGSEAKGMHFYLHVQQCELQHKELMIPRNFVERYWKDVPNPISLKLPNGSACKMNWVQRGDGIWLQNWKRFAWSLRCGDLLVFQYKGGSDFQVIILDDSKLEIDYSSMRCNNDQENTKESDDDDDDDDEYECVEIPSDTENTKIPLKKKRTNSNGIATTAATPQGTNIHKRKTNINATQQKVSDANGNTGGMGKNAKNAQKLNERSCTNSHPNESPSFTVKLSPYHVEGDNRLPIPSWFSKEYMNELLQGKATIRSVGEDSAWHVTLNFQKSRSSFVMRAGWKSFSKEHELKVGDVCKFEMTQREPLSFTITIIPTTKEPGPEKFQDSVSLNLHEMFKVFITSSDKLVVLPSELLKKHGIFSGNSVELRVGEGTWFVDVSYNEHLDYGWFTKGWQEFVRDCKVKTGDTCLFETIDVQNHVFKVSIV*