AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0068130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0068130 MtrunA17_Chr1g0164761 87.952 581 49 4 1 580 1 561 0.0 1047
Msa0068130 MtrunA17_Chr1g0164731 76.614 573 105 4 2 572 39 584 0.0 873
Msa0068130 MtrunA17_Chr5g0410721 52.610 498 230 2 2 494 83 579 0.0 552
Msa0068130 MtrunA17_Chr3g0116441 38.611 360 185 8 8 346 18 362 2.16e-68 241
Msa0068130 MtrunA17_Chr8g0380981 31.551 561 276 14 4 477 39 578 2.83e-58 210
Msa0068130 MtrunA17_Chr7g0214951 78.333 120 8 2 181 299 99 201 6.46e-57 190
Msa0068130 MtrunA17_Chr1g0189211 30.168 537 282 16 6 463 148 670 6.11e-55 201
Msa0068130 MtrunA17_Chr1g0199511 46.277 188 97 1 152 339 291 474 1.45e-53 197
Msa0068130 MtrunA17_Chr5g0397501 42.642 265 122 7 109 354 323 576 8.66e-52 192
Msa0068130 MtrunA17_Chr2g0303101 32.759 406 200 9 4 344 48 445 9.87e-52 191
Msa0068130 MtrunA17_Chr7g0261401 30.631 333 184 12 156 462 338 649 5.35e-30 125
Msa0068130 MtrunA17_Chr7g0214941 75.342 73 17 1 470 541 2 74 2.84e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0068130 AT5G46910.1 54.936 466 209 1 7 472 102 566 0.0 542
Msa0068130 AT5G46910.2 54.936 466 209 1 7 472 102 566 0.0 542
Msa0068130 AT2G34880.1 31.332 533 277 15 7 467 60 575 1.79e-59 212
Msa0068130 AT3G48430.1 35.326 368 196 6 9 339 21 383 2.09e-59 214
Msa0068130 AT1G63490.1 36.533 375 169 11 94 431 53 395 3.94e-57 208
Msa0068130 AT1G30810.3 29.870 539 290 13 4 467 55 580 3.97e-57 206
Msa0068130 AT1G30810.2 29.870 539 290 13 4 467 55 580 3.97e-57 206
Msa0068130 AT1G30810.1 29.870 539 290 13 4 467 55 580 3.97e-57 206
Msa0068130 AT1G63490.2 36.533 375 169 11 94 431 306 648 4.77e-57 208
Msa0068130 AT1G63490.4 36.533 375 169 11 94 431 306 648 4.91e-57 207
Msa0068130 AT4G20400.1 28.649 555 285 16 4 470 52 583 1.58e-56 205
Msa0068130 AT4G20400.2 27.907 602 317 18 16 529 7 579 1.93e-53 196
Msa0068130 AT5G04240.1 46.809 188 96 1 152 339 259 442 1.60e-52 194
Msa0068130 AT1G08620.3 40.417 240 123 4 117 343 327 559 1.64e-45 173
Msa0068130 AT1G08620.2 40.417 240 123 4 117 343 313 545 1.67e-45 173
Msa0068130 AT1G08620.4 40.417 240 123 4 117 343 313 545 1.67e-45 173
Msa0068130 AT1G08620.1 40.417 240 123 4 117 343 313 545 1.67e-45 173
Msa0068130 AT2G38950.1 25.912 521 312 15 2 466 102 604 2.24e-34 139
Msa0068130 AT1G63490.3 34.848 198 86 5 254 431 15 189 2.82e-24 108

Find 87 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCATTCATTTATGTTAT+TGG 0.159815 1_2:-33675589 None:intergenic
ACACAATTGTACAGCCTCTT+AGG 0.195974 1_2:+33673332 Msa0068130:CDS
TTGGAAAATATATTTGTTAA+AGG 0.217464 1_2:-33675570 None:intergenic
AATCCAGCATGATATGAATT+TGG 0.254423 1_2:-33674430 None:intergenic
TTCTTTACCACGCAATCTAT+TGG 0.264442 1_2:-33673541 None:intergenic
TGTCTTAACGGAAGAGAAAA+AGG 0.267528 1_2:+33673297 Msa0068130:CDS
TCCTTCAATATCTTCCCTTT+TGG 0.303945 1_2:+33676028 Msa0068130:CDS
ACTGAAAACAGAATAATAAT+TGG 0.313275 1_2:+33675787 Msa0068130:intron
AGTTCCTCATATGGAATTAT+AGG 0.316333 1_2:-33675048 None:intergenic
GAGAAAGGAACAGTTGAGTA+TGG 0.324983 1_2:+33673618 Msa0068130:CDS
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1_2:-33675057 None:intergenic
ATATAGGACTCGTTGTCTTT+TGG 0.352203 1_2:-33675967 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.359872 1_2:-33675333 None:intergenic
AGCTCTTCAAATACATCATT+AGG 0.370354 1_2:+33675237 Msa0068130:CDS
TGACATGCAGCCTCTGCAAT+AGG 0.375602 1_2:+33675265 Msa0068130:CDS
AACAAGCAATTTCAATTTGA+AGG 0.380018 1_2:+33673692 Msa0068130:CDS
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1_2:+33674974 Msa0068130:CDS
CTCCATTGCGTTTAGTTGAC+AGG 0.390855 1_2:+33673899 Msa0068130:CDS
GACAAAAGAAGCAGGATTAG+AGG 0.392437 1_2:-33673278 None:intergenic
TTGGTGATTGGTTTCCACTT+GGG 0.409363 1_2:+33674986 Msa0068130:CDS
GGAAGATATTGAAGGAACTT+TGG 0.410410 1_2:-33676021 None:intergenic
TGCTGCCAAAAGTCTTCAAC+AGG 0.414775 1_2:+33675524 Msa0068130:CDS
CTGATCCTATGCTTTACATT+GGG 0.424948 1_2:+33674023 Msa0068130:CDS
CACAATTGTACAGCCTCTTA+GGG 0.435355 1_2:+33673333 Msa0068130:CDS
CTTGTTCCAAGCTTATCATC+AGG 0.440827 1_2:-33673675 None:intergenic
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1_2:+33674967 Msa0068130:CDS
TTTGATTCGAAAACCCTAAG+AGG 0.457545 1_2:-33673346 None:intergenic
GCTACCTATAATTCCATATG+AGG 0.458631 1_2:+33675044 Msa0068130:CDS
TTAAAAGAACATGGAGAGAA+TGG 0.460534 1_2:+33674292 Msa0068130:CDS
TAAAAGAACATGGAGAGAAT+GGG 0.463284 1_2:+33674293 Msa0068130:CDS
TGGTGATTGGTTTCCACTTG+GGG 0.467102 1_2:+33674987 Msa0068130:CDS
AATTGAGAGGCTGCAGAACT+AGG 0.476304 1_2:-33674229 None:intergenic
TGCTCAAACTCTTGTTCTGA+TGG 0.476367 1_2:-33672773 None:intergenic
ATACCAAATTTAGAAGCCTC+GGG 0.480285 1_2:-33672824 None:intergenic
TAACTTGAGAAAACATGAAT+CGG 0.480484 1_2:+33675989 Msa0068130:CDS
TACAAGGCGGTGCAAAAGCC+AGG 0.481676 1_2:+33674388 Msa0068130:CDS
ACATAATGTGCCGGTTTACA+AGG 0.487938 1_2:+33674372 Msa0068130:CDS
TTGAAGATCACTATTTGTAC+AGG 0.488775 1_2:+33674074 Msa0068130:CDS
ATTGGTGATTGGTTTCCACT+TGG 0.491227 1_2:+33674985 Msa0068130:CDS
ATTTGAAGCTCTTGCAAACA+AGG 0.496352 1_2:+33673512 Msa0068130:CDS
TTTCCAAATTCATATCATGC+TGG 0.496493 1_2:+33674427 Msa0068130:CDS
TGCCACAAAATTGTTTATGC+GGG 0.497644 1_2:+33675454 Msa0068130:CDS
GACATGCAGCCTCTGCAATA+GGG 0.498995 1_2:+33675266 Msa0068130:CDS
CATACCAAATTTAGAAGCCT+CGG 0.500972 1_2:-33672825 None:intergenic
ATCATCAGGATCACTTGAAA+AGG 0.501772 1_2:-33673661 None:intergenic
GGTGCAAATAAAACTTGGTA+TGG 0.503293 1_2:+33674202 Msa0068130:CDS
ACGAGGTCTTACCATGGAAA+AGG 0.511206 1_2:-33675341 None:intergenic
GAAATAGTACATGGAGAGAA+AGG 0.511259 1_2:+33673603 Msa0068130:CDS
CAAAATCAAACTTGAAGATA+CGG 0.514277 1_2:+33675122 Msa0068130:CDS
ACTGATCCTATGCTTTACAT+TGG 0.515442 1_2:+33674022 Msa0068130:CDS
AGTGATCCTGATGATAAGCT+TGG 0.516084 1_2:+33673669 Msa0068130:CDS
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1_2:+33674047 Msa0068130:CDS
TCCGTTAAGACAAAAGAAGC+AGG 0.523048 1_2:-33673286 None:intergenic
TAAGACATCGCTATCGCAGT+TGG 0.526345 1_2:+33675194 Msa0068130:CDS
ATCTTCTGCAGATAAACTAA+AGG 0.527225 1_2:-33672797 None:intergenic
TTGGTACTCATCATCACTGA+TGG 0.536804 1_2:-33675948 None:intergenic
ACTCAGGTGCAAATAAAACT+TGG 0.537060 1_2:+33674197 Msa0068130:CDS
TTATGTTCTAACAAGACATC+AGG 0.538881 1_2:-33674355 None:intergenic
AGAAGAACTGTTAACTCGCT+AGG 0.545814 1_2:+33675828 Msa0068130:CDS
CTTGTTAGAACATAATGTGC+CGG 0.547471 1_2:+33674363 Msa0068130:CDS
AATGGAGACTGTGGCAGCCG+CGG 0.554838 1_2:-33673883 None:intergenic
GCAGGATTAGAGGCAGCGAT+TGG 0.560174 1_2:-33673268 None:intergenic
AAGGTGACGACAAACTCTCC+TGG 0.561754 1_2:-33674406 None:intergenic
CAAGACATCAGGAGAGAACA+TGG 0.563327 1_2:-33674344 None:intergenic
TGTTCTGATGGATAATACGT+TGG 0.567602 1_2:-33672761 None:intergenic
GCCAAAAGGGAAGATATTGA+AGG 0.571210 1_2:-33676029 None:intergenic
TTCCTGTCAACTAAACGCAA+TGG 0.577093 1_2:-33673901 None:intergenic
GTGCCACAAAATTGTTTATG+CGG 0.580719 1_2:+33675453 Msa0068130:CDS
TATCATGCTGGATTTAGTCA+TGG 0.587724 1_2:+33674439 Msa0068130:CDS
TTGCTAGCTGCAGCCCCAAG+TGG 0.592133 1_2:-33675000 None:intergenic
TTCTGGCACGAAATAGTACA+TGG 0.597269 1_2:+33673594 Msa0068130:CDS
ATTTAAGAGAAATGACATGT+TGG 0.608728 1_2:+33675491 Msa0068130:CDS
AAAAGAACATGGAGAGAATG+GGG 0.614492 1_2:+33674294 Msa0068130:CDS
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1_2:-33674028 None:intergenic
TACAACTCCTCAAAAGCTAG+AGG 0.624733 1_2:+33675096 Msa0068130:CDS
AGCATAAATTACCATCACTC+AGG 0.628951 1_2:+33674181 Msa0068130:intron
ACAGAATAATAATTGGCATG+AGG 0.629929 1_2:+33675794 Msa0068130:intron
AGCATGATATGAATTTGGAA+AGG 0.636839 1_2:-33674425 None:intergenic
AACAAAGTCTTAAAAGAACA+TGG 0.646276 1_2:+33674283 Msa0068130:CDS
CATAACAATCCCTATTGCAG+AGG 0.648673 1_2:-33675275 None:intergenic
TGAATGCATCAAAGAACACA+AGG 0.658928 1_2:+33675602 Msa0068130:CDS
ATTTGTTAAAGGTAACGCGT+CGG 0.660718 1_2:-33675559 None:intergenic
ACCAAATTTAGAAGCCTCGG+GGG 0.680089 1_2:-33672822 None:intergenic
TACCAAATTTAGAAGCCTCG+GGG 0.686654 1_2:-33672823 None:intergenic
ACTAAACGCAATGGAGACTG+TGG 0.698121 1_2:-33673892 None:intergenic
TAATGTGCCGGTTTACAAGG+CGG 0.701604 1_2:+33674375 Msa0068130:CDS
TGAGTATGGAGTCAATGTTG+AGG 0.703101 1_2:+33673632 Msa0068130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTTAATTCAAAATTTC+AGG + chr1_2:33673994-33674013 Msa0068130:intron 10.0%
!! TAATTTAATTCAAAATTTCA+GGG + chr1_2:33673995-33674014 Msa0068130:intron 10.0%
!! ATATTTGATCTTAATATTTC+AGG + chr1_2:33673226-33673245 Msa0068130:intron 15.0%
!!! AACAACTTTTAATTCATATT+AGG - chr1_2:33673955-33673974 None:intergenic 15.0%
!!! TTGGAAAATATATTTGTTAA+AGG - chr1_2:33675573-33675592 None:intergenic 15.0%
!! AGAACGATATAATCTAATAT+AGG + chr1_2:33673135-33673154 Msa0068130:intron 20.0%
!!! TAACTTTTTCTAAGAGAAAA+AGG + chr1_2:33673383-33673402 Msa0068130:CDS 20.0%
!! TGATGAAAAATTAACTAACT+TGG + chr1_2:33673449-33673468 Msa0068130:intron 20.0%
!!! CTTATATTTTACGAATAAGT+AGG + chr1_2:33674551-33674570 Msa0068130:intron 20.0%
!!! AATCTGTTTATTTTTGTACA+AGG + chr1_2:33674723-33674742 Msa0068130:intron 20.0%
!!! TTTTTTTCCATAATAATACC+TGG + chr1_2:33674803-33674822 Msa0068130:intron 20.0%
!!! ACATAAGTATTTAAAATAGC+AGG - chr1_2:33674842-33674861 None:intergenic 20.0%
!!! ACTATTTTAATGAAGTTTAG+AGG - chr1_2:33675671-33675690 None:intergenic 20.0%
!!! ATCTGATTGATTTTAACTTT+TGG + chr1_2:33675757-33675776 Msa0068130:intron 20.0%
!! ACTGAAAACAGAATAATAAT+TGG + chr1_2:33675787-33675806 Msa0068130:intron 20.0%
! ATAAAAAAACCCTAGAAACA+TGG - chr1_2:33672885-33672904 None:intergenic 25.0%
! AGTAGAATTCTTACTATACT+TGG - chr1_2:33672949-33672968 None:intergenic 25.0%
!! AGATTTTACTAAAATCACAG+TGG + chr1_2:33673096-33673115 Msa0068130:intron 25.0%
! AACAAGCAATTTCAATTTGA+AGG + chr1_2:33673692-33673711 Msa0068130:CDS 25.0%
! AACAAAGTCTTAAAAGAACA+TGG + chr1_2:33674283-33674302 Msa0068130:CDS 25.0%
!!! TTGTTTTTTGTGCAAGAAAT+TGG - chr1_2:33674324-33674343 None:intergenic 25.0%
!! AGGTTGATTGAATAATAGAA+AGG + chr1_2:33674575-33674594 Msa0068130:intron 25.0%
!! TGAATAATAGAAAGGTACAT+AGG + chr1_2:33674583-33674602 Msa0068130:intron 25.0%
! CTTTCTATTATTCAAGCTTA+GGG - chr1_2:33674630-33674649 None:intergenic 25.0%
!! ATCATTTTCTTGTTGAGTTT+AGG + chr1_2:33674931-33674950 Msa0068130:intron 25.0%
! CAAAATCAAACTTGAAGATA+CGG + chr1_2:33675122-33675141 Msa0068130:CDS 25.0%
! TGTCTGAATATAACCATAAT+AGG - chr1_2:33675431-33675450 None:intergenic 25.0%
! ATTTAAGAGAAATGACATGT+TGG + chr1_2:33675491-33675510 Msa0068130:CDS 25.0%
! GATGCATTCATTTATGTTAT+TGG - chr1_2:33675592-33675611 None:intergenic 25.0%
!! CATGTTTTCTCAAGTTATAT+AGG - chr1_2:33675986-33676005 None:intergenic 25.0%
! TAACTTGAGAAAACATGAAT+CGG + chr1_2:33675989-33676008 Msa0068130:CDS 25.0%
ATCTTCTGCAGATAAACTAA+AGG - chr1_2:33672800-33672819 None:intergenic 30.0%
!! TTTTCATCACCATGTTTCTA+GGG + chr1_2:33672873-33672892 Msa0068130:intron 30.0%
AAAGAAAGTTGAGGGATAAA+AGG - chr1_2:33672983-33673002 None:intergenic 30.0%
TACGTGCATGTTTAGATTAA+AGG + chr1_2:33673030-33673049 Msa0068130:intron 30.0%
!! TAGATATCGAAAAAGCATTC+TGG + chr1_2:33673577-33673596 Msa0068130:CDS 30.0%
!!! TTTGATTTTAGAATTTGCCG+CGG + chr1_2:33673866-33673885 Msa0068130:intron 30.0%
TACAAAAATTGCTTCCTTAC+TGG - chr1_2:33673931-33673950 None:intergenic 30.0%
TTGAAGATCACTATTTGTAC+AGG + chr1_2:33674074-33674093 Msa0068130:CDS 30.0%
TTAAAAGAACATGGAGAGAA+TGG + chr1_2:33674292-33674311 Msa0068130:CDS 30.0%
TAAAAGAACATGGAGAGAAT+GGG + chr1_2:33674293-33674312 Msa0068130:CDS 30.0%
TTATGTTCTAACAAGACATC+AGG - chr1_2:33674358-33674377 None:intergenic 30.0%
AGCATGATATGAATTTGGAA+AGG - chr1_2:33674428-33674447 None:intergenic 30.0%
TTTCCAAATTCATATCATGC+TGG + chr1_2:33674427-33674446 Msa0068130:CDS 30.0%
AATCCAGCATGATATGAATT+TGG - chr1_2:33674433-33674452 None:intergenic 30.0%
! TATTTTACGAATAAGTAGGC+AGG + chr1_2:33674555-33674574 Msa0068130:intron 30.0%
! AGAAAGGTACATAGGTTTAA+AGG + chr1_2:33674591-33674610 Msa0068130:intron 30.0%
CCTTTCTATTATTCAAGCTT+AGG - chr1_2:33674631-33674650 None:intergenic 30.0%
CCTAAGCTTGAATAATAGAA+AGG + chr1_2:33674628-33674647 Msa0068130:intron 30.0%
GTAGTAACTATTAGAACTTC+AGG - chr1_2:33674749-33674768 None:intergenic 30.0%
! TTTTCCATAATAATACCTGG+CGG + chr1_2:33674806-33674825 Msa0068130:intron 30.0%
!!! TTGAGTTTAGGTTTTAACTG+TGG + chr1_2:33674943-33674962 Msa0068130:intron 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG + chr1_2:33674974-33674993 Msa0068130:CDS 30.0%
AGTTCCTCATATGGAATTAT+AGG - chr1_2:33675051-33675070 None:intergenic 30.0%
TTACAAAGAAGTTCCTCATA+TGG - chr1_2:33675060-33675079 None:intergenic 30.0%
AGCTCTTCAAATACATCATT+AGG + chr1_2:33675237-33675256 Msa0068130:CDS 30.0%
AATACTCAAAGAGAATAACG+AGG - chr1_2:33675361-33675380 None:intergenic 30.0%
! TAAGTTGCTTGTGTTATTGA+TGG + chr1_2:33675396-33675415 Msa0068130:intron 30.0%
ATGGATAAACTTGCCTATTA+TGG + chr1_2:33675415-33675434 Msa0068130:intron 30.0%
! TTTTTCCTGTTGAAGACTTT+TGG - chr1_2:33675532-33675551 None:intergenic 30.0%
AAACACATTGCTTTATCTTC+AGG + chr1_2:33675718-33675737 Msa0068130:intron 30.0%
ACAGAATAATAATTGGCATG+AGG + chr1_2:33675794-33675813 Msa0068130:intron 30.0%
! TGTTCTGATGGATAATACGT+TGG - chr1_2:33672764-33672783 None:intergenic 35.0%
ATACCAAATTTAGAAGCCTC+GGG - chr1_2:33672827-33672846 None:intergenic 35.0%
CATACCAAATTTAGAAGCCT+CGG - chr1_2:33672828-33672847 None:intergenic 35.0%
! CTTTTCATCACCATGTTTCT+AGG + chr1_2:33672872-33672891 Msa0068130:intron 35.0%
TGGATGCAAAAGAAAGTTGA+GGG - chr1_2:33672991-33673010 None:intergenic 35.0%
ATGGATGCAAAAGAAAGTTG+AGG - chr1_2:33672992-33673011 None:intergenic 35.0%
! GCAACAGATTCTGCATTTTT+AGG + chr1_2:33673170-33673189 Msa0068130:intron 35.0%
! TCCTGCTTCTTTTGTCTTAA+CGG + chr1_2:33673285-33673304 Msa0068130:CDS 35.0%
TGTCTTAACGGAAGAGAAAA+AGG + chr1_2:33673297-33673316 Msa0068130:CDS 35.0%
! TTTGATTCGAAAACCCTAAG+AGG - chr1_2:33673349-33673368 None:intergenic 35.0%
! GGTTTTCGAATCAAATGAGA+TGG + chr1_2:33673354-33673373 Msa0068130:CDS 35.0%
! ATTTGAAGCTCTTGCAAACA+AGG + chr1_2:33673512-33673531 Msa0068130:CDS 35.0%
TTCTTTACCACGCAATCTAT+TGG - chr1_2:33673544-33673563 None:intergenic 35.0%
GAAATAGTACATGGAGAGAA+AGG + chr1_2:33673603-33673622 Msa0068130:CDS 35.0%
ATCATCAGGATCACTTGAAA+AGG - chr1_2:33673664-33673683 None:intergenic 35.0%
TTGACAGGAAAATTCCAGTA+AGG + chr1_2:33673914-33673933 Msa0068130:intron 35.0%
ACTGATCCTATGCTTTACAT+TGG + chr1_2:33674022-33674041 Msa0068130:CDS 35.0%
CTGATCCTATGCTTTACATT+GGG + chr1_2:33674023-33674042 Msa0068130:CDS 35.0%
TGCTATTCAGTATGTTTGCA+TGG + chr1_2:33674047-33674066 Msa0068130:CDS 35.0%
AGCATAAATTACCATCACTC+AGG + chr1_2:33674181-33674200 Msa0068130:intron 35.0%
!! TTTTATTTGCACCTGAGTGA+TGG - chr1_2:33674195-33674214 None:intergenic 35.0%
ACTCAGGTGCAAATAAAACT+TGG + chr1_2:33674197-33674216 Msa0068130:CDS 35.0%
GGTGCAAATAAAACTTGGTA+TGG + chr1_2:33674202-33674221 Msa0068130:CDS 35.0%
! GACAGTCTTTTCAAATTGAG+AGG - chr1_2:33674245-33674264 None:intergenic 35.0%
AAAAGAACATGGAGAGAATG+GGG + chr1_2:33674294-33674313 Msa0068130:CDS 35.0%
CTTGTTAGAACATAATGTGC+CGG + chr1_2:33674363-33674382 Msa0068130:CDS 35.0%
TATCATGCTGGATTTAGTCA+TGG + chr1_2:33674439-33674458 Msa0068130:CDS 35.0%
!! TGGTGTTATTACGTTTCTGA+TGG + chr1_2:33674522-33674541 Msa0068130:intron 35.0%
CGAGATTTGATTATGGAGTT+CGG - chr1_2:33674691-33674710 None:intergenic 35.0%
AATTCCGCCAGGTATTATTA+TGG - chr1_2:33674813-33674832 None:intergenic 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG + chr1_2:33674967-33674986 Msa0068130:CDS 35.0%
GCTACCTATAATTCCATATG+AGG + chr1_2:33675044-33675063 Msa0068130:CDS 35.0%
TTACCATGGAAAAGGCAAAT+TGG - chr1_2:33675336-33675355 None:intergenic 35.0%
GTGCCACAAAATTGTTTATG+CGG + chr1_2:33675453-33675472 Msa0068130:CDS 35.0%
TGCCACAAAATTGTTTATGC+GGG + chr1_2:33675454-33675473 Msa0068130:CDS 35.0%
! ATTTGTTAAAGGTAACGCGT+CGG - chr1_2:33675562-33675581 None:intergenic 35.0%
TGAATGCATCAAAGAACACA+AGG + chr1_2:33675602-33675621 Msa0068130:CDS 35.0%
! ATATAGGACTCGTTGTCTTT+TGG - chr1_2:33675970-33675989 None:intergenic 35.0%
GGAAGATATTGAAGGAACTT+TGG - chr1_2:33676024-33676043 None:intergenic 35.0%
! TCCTTCAATATCTTCCCTTT+TGG + chr1_2:33676028-33676047 Msa0068130:CDS 35.0%
!! TTTTTCAGATGTCGCCAAAA+GGG - chr1_2:33676045-33676064 None:intergenic 35.0%
!! ATTTTTCAGATGTCGCCAAA+AGG - chr1_2:33676046-33676065 None:intergenic 35.0%
TGCTCAAACTCTTGTTCTGA+TGG - chr1_2:33672776-33672795 None:intergenic 40.0%
TACCAAATTTAGAAGCCTCG+GGG - chr1_2:33672826-33672845 None:intergenic 40.0%
GACAAAAGAAGCAGGATTAG+AGG - chr1_2:33673281-33673300 None:intergenic 40.0%
TCCGTTAAGACAAAAGAAGC+AGG - chr1_2:33673289-33673308 None:intergenic 40.0%
ACACAATTGTACAGCCTCTT+AGG + chr1_2:33673332-33673351 Msa0068130:CDS 40.0%
CACAATTGTACAGCCTCTTA+GGG + chr1_2:33673333-33673352 Msa0068130:CDS 40.0%
TTCTGGCACGAAATAGTACA+TGG + chr1_2:33673594-33673613 Msa0068130:CDS 40.0%
GAGAAAGGAACAGTTGAGTA+TGG + chr1_2:33673618-33673637 Msa0068130:CDS 40.0%
TGAGTATGGAGTCAATGTTG+AGG + chr1_2:33673632-33673651 Msa0068130:CDS 40.0%
AGTGATCCTGATGATAAGCT+TGG + chr1_2:33673669-33673688 Msa0068130:CDS 40.0%
CTTGTTCCAAGCTTATCATC+AGG - chr1_2:33673678-33673697 None:intergenic 40.0%
TTCCTGTCAACTAAACGCAA+TGG - chr1_2:33673904-33673923 None:intergenic 40.0%
AGCATCCCAATGTAAAGCAT+AGG - chr1_2:33674031-33674050 None:intergenic 40.0%
!! ACATAATGTGCCGGTTTACA+AGG + chr1_2:33674372-33674391 Msa0068130:CDS 40.0%
GAACACGCGAGATTTGATTA+TGG - chr1_2:33674698-33674717 None:intergenic 40.0%
!! ATTGGTGATTGGTTTCCACT+TGG + chr1_2:33674985-33675004 Msa0068130:CDS 40.0%
!! TTGGTGATTGGTTTCCACTT+GGG + chr1_2:33674986-33675005 Msa0068130:CDS 40.0%
TACAACTCCTCAAAAGCTAG+AGG + chr1_2:33675096-33675115 Msa0068130:CDS 40.0%
!!! TTTGTCACCTCTAGCTTTTG+AGG - chr1_2:33675106-33675125 None:intergenic 40.0%
CATAACAATCCCTATTGCAG+AGG - chr1_2:33675278-33675297 None:intergenic 40.0%
! GATCCAATTTGCCTTTTCCA+TGG + chr1_2:33675330-33675349 Msa0068130:CDS 40.0%
AGAATAACGAGGTCTTACCA+TGG - chr1_2:33675350-33675369 None:intergenic 40.0%
! CTCCCGCATAAACAATTTTG+TGG - chr1_2:33675459-33675478 None:intergenic 40.0%
AGAAGAACTGTTAACTCGCT+AGG + chr1_2:33675828-33675847 Msa0068130:CDS 40.0%
TTGGTACTCATCATCACTGA+TGG - chr1_2:33675951-33675970 None:intergenic 40.0%
GCCAAAAGGGAAGATATTGA+AGG - chr1_2:33676032-33676051 None:intergenic 40.0%
ACCAAATTTAGAAGCCTCGG+GGG - chr1_2:33672825-33672844 None:intergenic 45.0%
AAACTCGAGCACTTCAGAGA+TGG - chr1_2:33673011-33673030 None:intergenic 45.0%
! GCGTTTTCCAATAGATTGCG+TGG + chr1_2:33673534-33673553 Msa0068130:CDS 45.0%
ACTAAACGCAATGGAGACTG+TGG - chr1_2:33673895-33673914 None:intergenic 45.0%
CTCCATTGCGTTTAGTTGAC+AGG + chr1_2:33673899-33673918 Msa0068130:CDS 45.0%
AATTGAGAGGCTGCAGAACT+AGG - chr1_2:33674232-33674251 None:intergenic 45.0%
CAAGACATCAGGAGAGAACA+TGG - chr1_2:33674347-33674366 None:intergenic 45.0%
!! TAATGTGCCGGTTTACAAGG+CGG + chr1_2:33674375-33674394 Msa0068130:CDS 45.0%
! TTTTGCACCGCCTTGTAAAC+CGG - chr1_2:33674385-33674404 None:intergenic 45.0%
CTCTCTCTCTCTACATGTGT+TGG + chr1_2:33674502-33674521 Msa0068130:intron 45.0%
!! TGGTGATTGGTTTCCACTTG+GGG + chr1_2:33674987-33675006 Msa0068130:CDS 45.0%
TAAGACATCGCTATCGCAGT+TGG + chr1_2:33675194-33675213 Msa0068130:CDS 45.0%
ACGAGGTCTTACCATGGAAA+AGG - chr1_2:33675344-33675363 None:intergenic 45.0%
TGCTGCCAAAAGTCTTCAAC+AGG + chr1_2:33675524-33675543 Msa0068130:CDS 45.0%
AAGGTGACGACAAACTCTCC+TGG - chr1_2:33674409-33674428 None:intergenic 50.0%
TGCAGCAGCAGAGGAACAAT+AGG + chr1_2:33674665-33674684 Msa0068130:intron 50.0%
TGACATGCAGCCTCTGCAAT+AGG + chr1_2:33675265-33675284 Msa0068130:CDS 50.0%
GACATGCAGCCTCTGCAATA+GGG + chr1_2:33675266-33675285 Msa0068130:CDS 50.0%
GCCCCCGAGGCTTCTAAATT+TGG + chr1_2:33672821-33672840 Msa0068130:CDS 55.0%
GCAGGATTAGAGGCAGCGAT+TGG - chr1_2:33673271-33673290 None:intergenic 55.0%
TACAAGGCGGTGCAAAAGCC+AGG + chr1_2:33674388-33674407 Msa0068130:CDS 55.0%
GTTCCTCTGCTGCTGCATAG+TGG - chr1_2:33674662-33674681 None:intergenic 55.0%
GCAGGAAGTGAAATTCCGCC+AGG - chr1_2:33674824-33674843 None:intergenic 55.0%
TCTGCAGAAGATTGCCCCCG+AGG + chr1_2:33672808-33672827 Msa0068130:CDS 60.0%
AATGGAGACTGTGGCAGCCG+CGG - chr1_2:33673886-33673905 None:intergenic 60.0%
CCTCTGCTGCTGCATAGTGG+CGG - chr1_2:33674659-33674678 None:intergenic 60.0%
TTGCTAGCTGCAGCCCCAAG+TGG - chr1_2:33675003-33675022 None:intergenic 60.0%
CCGCCACTATGCAGCAGCAG+AGG + chr1_2:33674656-33674675 Msa0068130:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 33672737 33676073 33672737 ID=Msa0068130;Name=Msa0068130
chr1_2 mRNA 33672737 33676073 33672737 ID=Msa0068130-mRNA-1;Parent=Msa0068130;Name=Msa0068130-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.12|0|1|1|1|9|0|581
chr1_2 exon 33672737 33672842 33672737 ID=Msa0068130-mRNA-1:exon:11064;Parent=Msa0068130-mRNA-1
chr1_2 exon 33673248 33673404 33673248 ID=Msa0068130-mRNA-1:exon:11065;Parent=Msa0068130-mRNA-1
chr1_2 exon 33673494 33673713 33673494 ID=Msa0068130-mRNA-1:exon:11066;Parent=Msa0068130-mRNA-1
chr1_2 exon 33673877 33673930 33673877 ID=Msa0068130-mRNA-1:exon:11067;Parent=Msa0068130-mRNA-1
chr1_2 exon 33674016 33674095 33674016 ID=Msa0068130-mRNA-1:exon:11068;Parent=Msa0068130-mRNA-1
chr1_2 exon 33674183 33674460 33674183 ID=Msa0068130-mRNA-1:exon:11069;Parent=Msa0068130-mRNA-1
chr1_2 exon 33674953 33675351 33674953 ID=Msa0068130-mRNA-1:exon:11070;Parent=Msa0068130-mRNA-1
chr1_2 exon 33675448 33675623 33675448 ID=Msa0068130-mRNA-1:exon:11071;Parent=Msa0068130-mRNA-1
chr1_2 exon 33675798 33676073 33675798 ID=Msa0068130-mRNA-1:exon:11072;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33672737 33672842 33672737 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33673248 33673404 33673248 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33673494 33673713 33673494 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33673877 33673930 33673877 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33674016 33674095 33674016 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33674183 33674460 33674183 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33674953 33675351 33674953 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33675448 33675623 33675448 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
chr1_2 CDS 33675798 33676073 33675798 ID=Msa0068130-mRNA-1:cds;Parent=Msa0068130-mRNA-1
Gene Sequence

>Msa0068130

ATGACGAACATTACTCAAGAATGTCCAACGTATTATCCATCAGAACAAGAGTTTGAGCATCCTTTAGTTTATCTGCAGAAGATTGCCCCCGAGGCTTCTAAATTTGGTATATGCAAAATTGTTTCACCAATCGCTGCCTCTAATCCTGCTTCTTTTGTCTTAACGGAAGAGAAAAAGGATTTTAAGTTTAACACAATTGTACAGCCTCTTAGGGTTTTCGAATCAAATGAGATGGATAAAATAACTTTTTCTAAGAGAAAAAGAAAATATACATATCATGAATTTGAAGCTCTTGCAAACAAGGCGTTTTCCAATAGATTGCGTGGTAAAGAAGATCTCTCTTGTTTAGATATCGAAAAAGCATTCTGGCACGAAATAGTACATGGAGAGAAAGGAACAGTTGAGTATGGAGTCAATGTTGAGGAAAGCGCCTTTTCAAGTGATCCTGATGATAAGCTTGGAACAAGCAATTTCAATTTGAAGAATTTGCCGCGGCTGCCACAGTCTCCATTGCGTTTAGTTGACAGGAAAATTCCAGGATTAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTTGAAGATCACTATTTGTACAGCATAAATTACCATCACTCAGGTGCAAATAAAACTTGGTATGGTGTGCCTAGTTCTGCAGCCTCTCAATTTGAAAAGACTGTCTTGAATCATGTGTATTGCAACAAAGTCTTAAAAGAACATGGAGAGAATGGGGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTAGAACATAATGTGCCGGTTTACAAGGCGGTGCAAAAGCCAGGAGAGTTTGTCGTCACCTTTCCAAATTCATATCATGCTGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCACTTGGGGCTGCAGCTAGCAAACGTTATTCACATCTCAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCAAAAGCTAGAGGTGACAAAATCAAACTTGAAGATACGGCAACTTATCGAGCTATTGCACTATCTTTCTTGCATCTTATTCAATTTTATAAGACATCGCTATCGCAGTTGGACAGTTCGAAAAAGTTGTCAAGCTCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTCTGCAATAGGGATTGTTATGTAGCTTTCTTGTTGTGCAAGAAATGCTATTCAGATCCAATTTGCCTTTTCCATGACAATGTGCCACAAAATTGTTTATGCGGGAGAGAACATACTGTATTTAAGAGAAATGACATGTTGGAACTCGAAGCTGCTGCCAAAAGTCTTCAACAGGAAAAAGAATGCACCGACGCGTTACCTTTAACAAATATATTTTCCAATAACATAAATGAATGCATCAAAGAACACAAGAATAATAATTGGCATGAGGAAAAAAGTGCTAGAAGAACTGTTAACTCGCTAGGTGCAACATCTAAACTGAAAACAAGAGCTGCGAACAACATCAAGCACACTGTTGAAAATAATCAAAAGACAAAGAATCGTTGTAGAAACAATGTATCTCCATCAGTGATGATGAGTACCAAAAGACAACGAGTCCTATATAACTTGAGAAAACATGAATCGGAACTGATTACCAAAGTTCCTTCAATATCTTCCCTTTTGGCGACATCTGAAAAATCAAATTAA
Protein sequence

>Msa0068130

MTNITQECPTYYPSEQEFEHPLVYLQKIAPEASKFGICKIVSPIAASNPASFVLTEEKKDFKFNTIVQPLRVFESNEMDKITFSKRKRKYTYHEFEALANKAFSNRLRGKEDLSCLDIEKAFWHEIVHGEKGTVEYGVNVEESAFSSDPDDKLGTSNFNLKNLPRLPQSPLRLVDRKIPGLTDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGANKTWYGVPSSAASQFEKTVLNHVYCNKVLKEHGENGAFQFLAQKTTMFSPDVLLEHNVPVYKAVQKPGEFVVTFPNSYHAGFSHGFNCGEAVNFAIGDWFPLGAAASKRYSHLKMLPIIPYEELLCKEALLIYNSSKARGDKIKLEDTATYRAIALSFLHLIQFYKTSLSQLDSSKKLSSSSNTSLGSVTCSLCNRDCYVAFLLCKKCYSDPICLFHDNVPQNCLCGREHTVFKRNDMLELEAAAKSLQQEKECTDALPLTNIFSNNINECIKEHKNNNWHEEKSARRTVNSLGATSKLKTRAANNIKHTVENNQKTKNRCRNNVSPSVMMSTKRQRVLYNLRKHESELITKVPSISSLLATSEKSN*