AlfalfaGEDB | Msa0068330

M. sativa cultivar zhongmu-4 / Msa0068330

KEGG: K12592
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068330	AFK40772.1	95.263	190	9	0	1	190	1	190	6.71e-124	364

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068330	sp\|Q6LA43\|APRR2_ARATH	70.513	78	20	2	223	299	293	368	1.61e-27	115

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068330	I3SKI0	95.263	190	9	0	1	190	1	190	3.20e-124	364

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TFs/TRs:

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Gene ID	Type	Classification
Msa0068330	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068330	MtrunA17_Chr3g0136861	95.263	190	9	0	1	190	1	190	8.81e-128	364
Msa0068330	MtrunA17_Chr4g0054981	76.378	127	29	1	174	299	255	381	2.98e-58	196
Msa0068330	MtrunA17_Chr5g0400241	73.196	97	26	0	203	299	286	382	5.42e-40	147
Msa0068330	MtrunA17_Chr1g0150121	40.351	171	66	4	1	169	1	137	4.57e-27	105
Msa0068330	MtrunA17_Chr4g0040481	58.667	75	31	0	225	299	155	229	4.23e-26	107
Msa0068330	MtrunA17_Chr2g0324891	63.636	66	24	0	225	290	158	223	3.27e-25	104
Msa0068330	MtrunA17_Chr2g0304841	58.462	65	27	0	224	288	209	273	3.36e-19	88.2
Msa0068330	MtrunA17_Chr3g0137101	62.295	61	23	0	226	286	196	256	1.45e-18	86.3
Msa0068330	MtrunA17_Chr4g0067981	63.333	60	22	0	227	286	214	273	2.50e-18	85.9
Msa0068330	MtrunA17_Chr4g0074401	62.712	59	22	0	223	281	198	256	2.02e-17	82.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068330	AT5G25080.1	50.000	178	87	1	13	190	16	191	1.67e-59	190
Msa0068330	AT4G18020.8	70.513	78	20	2	223	299	293	368	1.45e-28	115
Msa0068330	AT4G18020.11	70.513	78	20	2	223	299	293	368	1.45e-28	115
Msa0068330	AT4G18020.4	70.513	78	20	2	223	299	293	368	1.45e-28	115
Msa0068330	AT4G18020.5	70.513	78	20	2	223	299	293	368	1.45e-28	115
Msa0068330	AT4G18020.10	70.513	78	20	2	223	299	293	368	1.45e-28	115
Msa0068330	AT4G18020.7	70.513	78	20	2	223	299	293	368	1.45e-28	115
Msa0068330	AT4G18020.3	70.513	78	20	2	223	299	293	368	1.64e-28	115
Msa0068330	AT4G18020.2	70.513	78	20	2	223	299	293	368	1.64e-28	115
Msa0068330	AT4G18020.1	70.513	78	20	2	223	299	293	368	1.64e-28	115

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Find 77 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGTTGAAAATGTGAA+CGG	0.603460	1_2:+34478500	None:intergenic
AAAATGAGGAACTTAAGTAG+TGG	0.647264	1_2:-34477513	Msa0068330:CDS
AACATAGTAGAAGGTCGCAA+TGG	0.635548	1_2:+34477710	None:intergenic
AACCCACCTGGAGTGAGGTC+AGG	0.514003	1_2:+34477647	None:intergenic
AAGAAACAAACCAGAGAAGC+CGG	0.460719	1_2:+34475165	None:intergenic
AATATTGGATCTAATGAAAG+TGG	0.519493	1_2:-34475943	Msa0068330:CDS
AATGTGAGATAAAAGCTAAT+CGG	0.462021	1_2:-34476216	Msa0068330:CDS
ACAACATCTGCAAATAAAGT+AGG	0.528162	1_2:-34476253	Msa0068330:CDS
ACGCCTTCTAGATTTGAATA+AGG	0.277390	1_2:-34478101	Msa0068330:CDS
ACGTTCAAGAGGTGGAAGTT+GGG	0.491157	1_2:+34478450	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATTTGACATGGTTATCATT+TGG	+	chr1_2:34475523-34475542	None:intergenic	30.0%
TACCCTTATTCAAATCTAGA+AGG	+	chr1_2:34475673-34475692	None:intergenic	30.0%
ACATGTTAACCTTCAATATG+AGG	-	chr1_2:34475932-34475951	Msa0068330:CDS	30.0%
AGGCTAAGCAAATAAAAAAG+AGG	+	chr1_2:34475978-34475997	None:intergenic	30.0%
TGAGAATTCAACATAGTAGA+AGG	+	chr1_2:34476070-34476089	None:intergenic	30.0%
TAACTTGGTTTATTGCTTCT+AGG	-	chr1_2:34476146-34476165	Msa0068330:intron	30.0%
AACTTGGTTTATTGCTTCTA+GGG	-	chr1_2:34476147-34476166	Msa0068330:intron	30.0%
TACATGCAATGTGATCAAAA+TGG	+	chr1_2:34476196-34476215	None:intergenic	30.0%
ATTGCATGTATGTAAAGAAG+TGG	-	chr1_2:34476206-34476225	Msa0068330:CDS	30.0%
AAAATGAGGAACTTAAGTAG+TGG	-	chr1_2:34476255-34476274	Msa0068330:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	34475176	34478614	34475176	ID=Msa0068330;Name=Msa0068330
chr1_2	mRNA	34475176	34478614	34475176	ID=Msa0068330-mRNA-1;Parent=Msa0068330;Name=Msa0068330-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0\|0\|0\|0.66\|0.87\|0.88\|9\|0\|324
chr1_2	exon	34478401	34478614	34478401	ID=Msa0068330-mRNA-1:exon:11207;Parent=Msa0068330-mRNA-1
chr1_2	exon	34478241	34478296	34478241	ID=Msa0068330-mRNA-1:exon:11206;Parent=Msa0068330-mRNA-1
chr1_2	exon	34478101	34478155	34478101	ID=Msa0068330-mRNA-1:exon:11205;Parent=Msa0068330-mRNA-1
chr1_2	exon	34477654	34477734	34477654	ID=Msa0068330-mRNA-1:exon:11204;Parent=Msa0068330-mRNA-1
chr1_2	exon	34477358	34477546	34477358	ID=Msa0068330-mRNA-1:exon:11203;Parent=Msa0068330-mRNA-1
chr1_2	exon	34476204	34476298	34476204	ID=Msa0068330-mRNA-1:exon:11202;Parent=Msa0068330-mRNA-1
chr1_2	exon	34475905	34476045	34475905	ID=Msa0068330-mRNA-1:exon:11201;Parent=Msa0068330-mRNA-1
chr1_2	exon	34475704	34475768	34475704	ID=Msa0068330-mRNA-1:exon:11200;Parent=Msa0068330-mRNA-1
chr1_2	exon	34475176	34475251	34475176	ID=Msa0068330-mRNA-1:exon:11199;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34478401	34478614	34478401	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34478241	34478296	34478241	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34478101	34478155	34478101	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34477654	34477734	34477654	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34477358	34477546	34477358	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34476204	34476298	34476204	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34475905	34476045	34475905	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34475704	34475768	34475704	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1
chr1_2	CDS	34475176	34475251	34475176	ID=Msa0068330-mRNA-1:cds;Parent=Msa0068330-mRNA-1

Gene Sequence

>Msa0068330

ATGGTGAAAGGAAGTGAAAGTGAAAGTTGTGGAGGAGTACCCGAAGCGGTGATGGATTCAGTCAACACCACTCTCTCCAATCTTCGTGAATTCCGTTCACATTTTCAACAGTTTTTACCTCTTATGAACCCTCAAACTCTCTCCCAACTTCCACCTCTTGAACGTGCCCACTCTCGCTTTCTCCTCTCCAAAATCACTTCTACCCTCCTCACACTGAAATTGAGGTGTAGTGGAGTTCAACCAAATGATAACCATGTCAAATCAGAGCTTGATAGAGTAGACTTATATCAAGACAAACTTGAACGCCTTCTAGATTTGAATAAGGCTCCATTGCGACCTTCTACTATGTTGAATTCTCAAGCAGCTACTGGCTTCATCGAACACTCTCTGCCTGACCTCACTCCAGAACAACGGCAGAAAATGAGGAACTTAAGTAGTGGCGAGGGAAAGAAGAGGAAACATCAGGAGCAGGCTGGTCAAAAGAGGAAGTATCAATCTTCTGAAAAGCCATCTATTTCTGTTCAAGCTGCTGCTCAGGAGTTTCTGGAGAAAGCACAGAGGGAACTTTTACATTTGTTTCATATGGATTGGATTGTGACAATATTTAATGAAAGTAATACAACATCTGCAAATAAAGTAGGTATTCGCAGTCATAAATGTGAGATAAAAGCTAATCGGAAGAAGATGAAAGTAGACTGGACACCAGAGCTGCACAAAAAATTTGTGAAGGCAGTGGAGCAACTAGGCATTGATCACGCTATTCCTTCTCGAATATTGGATCTAATGAAAGTGGAGGGCTTGACAAGGCATAATGTGGCAAGCCATCTTCAGAAATATAGAATGCATAAGAGACATATCTTACCGAAGCAGGATGATCGAAAATGGCTAAATACAAGGCTGGTTGGTGTTGGCGAGGCTTCATTTATAAGTTTAGCACCACCTTTCATTGATGACAACGCCCCGGCTTCTCTG

Protein sequence

>Msa0068330

MVKGSESESCGGVPEAVMDSVNTTLSNLREFRSHFQQFLPLMNPQTLSQLPPLERAHSRFLLSKITSTLLTLKLRCSGVQPNDNHVKSELDRVDLYQDKLERLLDLNKAPLRPSTMLNSQAATGFIEHSLPDLTPEQRQKMRNLSSGEGKKRKHQEQAGQKRKYQSSEKPSISVQAAAQEFLEKAQRELLHLFHMDWIVTIFNESNTTSANKVGIRSHKCEIKANRKKMKVDWTPELHKKFVKAVEQLGIDHAIPSRILDLMKVEGLTRHNVASHLQKYRMHKRHILPKQDDRKWLNTRLVGVGEASFISLAPPFIDDNAPASL