AlfalfaGEDB | Msa0069120

M. sativa cultivar zhongmu-4 / Msa0069120

KEGG: K02218
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069120	XP_003627159.1	85.799	169	18	3	1	166	1	166	4.41e-92	286

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069120	sp\|Q9CAI5\|CKL2_ARATH	79.290	169	29	3	1	166	1	166	3.68e-88	268

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069120	G7LI85	85.799	169	18	3	1	166	1	166	2.11e-92	286

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069120	MtrunA17_Chr8g0342821	85.799	169	18	3	1	166	1	166	4.05e-96	286
Msa0069120	MtrunA17_Chr4g0071031	78.824	170	29	4	1	166	1	167	1.04e-88	266
Msa0069120	MtrunA17_Chr2g0329981	80.000	170	27	4	1	166	1	167	1.09e-88	266
Msa0069120	MtrunA17_Chr2g0299661	73.529	170	38	4	1	166	1	167	1.66e-80	246
Msa0069120	MtrunA17_Chr1g0166821	67.273	165	49	3	5	166	6	168	2.12e-76	234
Msa0069120	MtrunA17_Chr1g0183631	65.089	169	54	3	1	166	1	167	7.81e-75	230
Msa0069120	MtrunA17_Chr7g0261501	63.905	169	56	3	1	166	1	167	3.50e-74	229
Msa0069120	MtrunA17_Chr1g0187571	65.089	169	54	3	1	166	2	168	2.42e-73	227
Msa0069120	MtrunA17_Chr6g0486611	65.294	170	52	4	1	166	1	167	8.32e-69	214
Msa0069120	MtrunA17_Chr8g0390211	61.765	170	58	4	1	166	1	167	3.12e-63	200

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0069120	AT1G72710.1	79.290	169	29	3	1	166	1	166	3.74e-89	268
Msa0069120	AT4G26100.1	77.059	170	32	4	1	166	1	167	2.76e-86	260
Msa0069120	AT2G19470.1	72.781	169	41	3	1	166	1	167	1.70e-84	255
Msa0069120	AT5G57015.1	74.706	170	36	4	1	166	1	167	2.10e-84	255
Msa0069120	AT1G03930.1	68.485	165	47	3	5	166	5	167	1.46e-77	238
Msa0069120	AT5G44100.1	66.667	165	50	3	5	166	5	167	1.84e-75	233
Msa0069120	AT5G44100.2	66.667	165	50	3	5	166	5	167	1.84e-75	233
Msa0069120	AT3G23340.1	62.722	169	58	3	1	166	1	167	1.84e-72	224
Msa0069120	AT5G43320.1	63.030	165	56	3	5	166	5	167	3.47e-72	225
Msa0069120	AT5G43320.2	62.130	169	59	3	1	166	1	167	4.18e-72	221

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Find 47 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGTAGCGGATCTTT+CGG	0.404618	1_2:-37025042	Msa0069120:CDS
AAACCCATGAGGAAATTGTC+TGG	0.531680	1_2:+37024138	None:intergenic
AAAGCCAGACAATTTCCTCA+TGG	0.544831	1_2:-37024142	Msa0069120:CDS
AACGATTGAGAGTGAGATTG+AGG	0.486175	1_2:+37025194	None:intergenic
AAGCCAGACAATTTCCTCAT+GGG	0.517628	1_2:-37024141	Msa0069120:CDS
AATATCCTTCAATCTCACTA+TGG	0.430325	1_2:-37025240	Msa0069120:five_prime_UTR
ACAATTTCCTCATGGGTTTA+GGG	0.340991	1_2:-37024134	Msa0069120:CDS
ACACCGGACCATTTCACATT+CGG	0.480710	1_2:+37024414	None:intergenic
AGAGTACAATATCCTAGTGA+TGG	0.570391	1_2:-37024385	Msa0069120:CDS
AGTGAGATTGAAGGATATTT+AGG	0.218210	1_2:+37025244	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTGAGATTGAAGGATATTT+AGG	+	chr1_2:37023971-37023990	None:intergenic	30.0%
AATATCCTTCAATCTCACTA+TGG	-	chr1_2:37023972-37023991	Msa0069120:CDS	30.0%
AAAGAGTTGTATAGGCAATA+TGG	-	chr1_2:37024510-37024529	Msa0069120:intron	30.0%
AAGAGTTGTATAGGCAATAT+GGG	-	chr1_2:37024511-37024530	Msa0069120:intron	30.0%
TATGGGATATTGAATTTCGT+TGG	-	chr1_2:37024528-37024547	Msa0069120:intron	30.0%
TATTACTCACTTGGCTTAAA+CGG	-	chr1_2:37024739-37024758	Msa0069120:intron	30.0%
TGAATAAATCCTCAAGACTA+GGG	+	chr1_2:37024863-37024882	None:intergenic	30.0%
TTGAATAAATCCTCAAGACT+AGG	+	chr1_2:37024864-37024883	None:intergenic	30.0%
TTACAAGCTTATGCAATACA+GGG	+	chr1_2:37024950-37024969	None:intergenic	30.0%
ATTACAAGCTTATGCAATAC+AGG	+	chr1_2:37024951-37024970	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	37023955	37025279	37023955	ID=Msa0069120;Name=Msa0069120
chr1_2	mRNA	37023955	37025279	37023955	ID=Msa0069120-mRNA-1;Parent=Msa0069120;Name=Msa0069120-mRNA-1;_AED=0.21;_eAED=0.22;_QI=176\|0.4\|0.33\|0.83\|0.8\|0.66\|6\|0\|166
chr1_2	exon	37023955	37024020	37023955	ID=Msa0069120-mRNA-1:exon:11713;Parent=Msa0069120-mRNA-1
chr1_2	exon	37024113	37024214	37024113	ID=Msa0069120-mRNA-1:exon:11712;Parent=Msa0069120-mRNA-1
chr1_2	exon	37024296	37024444	37024296	ID=Msa0069120-mRNA-1:exon:11711;Parent=Msa0069120-mRNA-1
chr1_2	exon	37024610	37024673	37024610	ID=Msa0069120-mRNA-1:exon:11710;Parent=Msa0069120-mRNA-1
chr1_2	exon	37024903	37024943	37024903	ID=Msa0069120-mRNA-1:exon:11709;Parent=Msa0069120-mRNA-1
chr1_2	exon	37025028	37025279	37025028	ID=Msa0069120-mRNA-1:exon:11708;Parent=Msa0069120-mRNA-1
chr1_2	five_prime_UTR	37025104	37025279	37025104	ID=Msa0069120-mRNA-1:five_prime_utr;Parent=Msa0069120-mRNA-1
chr1_2	CDS	37025028	37025103	37025028	ID=Msa0069120-mRNA-1:cds;Parent=Msa0069120-mRNA-1
chr1_2	CDS	37024903	37024943	37024903	ID=Msa0069120-mRNA-1:cds;Parent=Msa0069120-mRNA-1
chr1_2	CDS	37024610	37024673	37024610	ID=Msa0069120-mRNA-1:cds;Parent=Msa0069120-mRNA-1
chr1_2	CDS	37024296	37024444	37024296	ID=Msa0069120-mRNA-1:cds;Parent=Msa0069120-mRNA-1
chr1_2	CDS	37024113	37024214	37024113	ID=Msa0069120-mRNA-1:cds;Parent=Msa0069120-mRNA-1
chr1_2	CDS	37023955	37024020	37023955	ID=Msa0069120-mRNA-1:cds;Parent=Msa0069120-mRNA-1

Gene Sequence

>Msa0069120

ATGGAACCTCGAATTGGGAATAAGTTCCGTCTTGGGAGGAAAATTGGTAGCGGATCTTTCGGAGAGATCTATTTAGGTACCAATATTCAAACTAATGAAGAAGTCGCGATTAAGCTTTTTTTGATACTGAACTTGTTGGTTTATTTTCGCTTTCTAATTGAAGTATTGATTACAGAAGCAGCTGGGATACCGAATGTGAAATGGTCCGGTGTCGAAGGAGAGTACAATATCCTAGTGATGGATTTACTTGGCCCTAGTCTTGAGGATTTATTCAATTTCTGTAATAGGAAATTGTCTCTCAAAACTGTTCTTATGCTTGCTGATCAAATGATAAATCGAGCTGAATTTGTTCATACCAAGTCATTTTTACATCGGGACATAAAGCCAGACAATTTCCTCATGGGTTTAGGGAGGCGTGCAAATCAAGTACTTGTGTACATCATTGACTTTGGTCTTGCTAAGAAATTCAGAGACAGTGCACATCAGCATATTCCGTAC

Protein sequence

>Msa0069120

MEPRIGNKFRLGRKIGSGSFGEIYLGTNIQTNEEVAIKLFLILNLLVYFRFLIEVLITEAAGIPNVKWSGVEGEYNILVMDLLGPSLEDLFNFCNRKLSLKTVLMLADQMINRAEFVHTKSFLHRDIKPDNFLMGLGRRANQVLVYIIDFGLAKKFRDSAHQHIPY