AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0070260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0070260 MtrunA17_Chr1g0175071 93.478 322 19 1 1 322 1 320 0.0 610
Msa0070260 MtrunA17_Chr7g0258531 45.126 277 120 9 53 315 90 348 2.83e-54 181
Msa0070260 MtrunA17_Chr1g0175091 46.512 172 86 3 142 309 218 387 5.27e-35 131
Msa0070260 MtrunA17_Chr7g0258551 51.145 131 64 0 179 309 212 342 6.77e-34 128
Msa0070260 MtrunA17_Chr1g0190301 37.727 220 129 4 91 309 94 306 1.75e-33 127
Msa0070260 MtrunA17_Chr7g0261911 44.667 150 75 3 160 307 248 391 1.63e-32 125
Msa0070260 MtrunA17_Chr2g0291021 47.445 137 63 2 179 309 49 182 7.98e-31 118
Msa0070260 MtrunA17_Chr7g0274471 43.939 132 73 1 179 309 218 349 4.06e-28 113
Msa0070260 MtrunA17_Chr7g0254981 39.437 142 74 2 176 309 47 184 6.53e-26 104
Msa0070260 MtrunA17_Chr8g0356501 38.608 158 85 2 168 317 26 179 1.11e-24 102
Msa0070260 MtrunA17_Chr2g0321461 39.181 171 83 6 167 316 33 203 3.86e-24 101
Msa0070260 MtrunA17_Chr2g0310011 40.845 142 72 2 168 301 24 161 5.65e-24 100
Msa0070260 MtrunA17_Chr6g0461641 37.580 157 73 3 174 309 37 189 3.96e-23 98.6
Msa0070260 MtrunA17_Chr4g0042451 37.931 145 74 3 174 304 12 154 2.19e-20 90.1
Msa0070260 MtrunA17_Chr5g0409731 36.306 157 81 3 179 319 25 178 2.87e-20 89.4
Msa0070260 MtrunA17_Chr5g0417521 37.584 149 74 3 172 304 8 153 2.92e-20 90.1
Msa0070260 MtrunA17_Chr7g0242521 39.007 141 81 3 175 311 9 148 2.59e-18 82.4
Msa0070260 MtrunA17_Chr2g0290991 34.266 143 81 2 174 303 34 176 1.16e-17 82.0
Msa0070260 MtrunA17_Chr1g0197171 33.103 145 94 3 166 309 31 173 4.39e-16 77.4
Msa0070260 MtrunA17_Chr8g0379121 51.429 70 26 1 181 250 113 174 1.63e-14 73.9
Msa0070260 MtrunA17_Chr1g0162931 62.264 53 20 0 181 233 20 72 2.27e-14 72.4
Msa0070260 MtrunA17_Chr1g0175081 59.615 52 21 0 181 232 24 75 2.71e-14 67.8
Msa0070260 MtrunA17_Chr2g0320231 51.429 70 26 1 181 250 99 160 4.90e-14 72.0
Msa0070260 MtrunA17_Chr3g0095611 59.259 54 22 0 181 234 21 74 6.94e-14 69.7
Msa0070260 MtrunA17_Chr1g0210911 60.377 53 21 0 181 233 24 76 8.77e-14 71.2
Msa0070260 MtrunA17_Chr8g0366281 34.815 135 77 3 157 280 146 280 1.17e-13 70.9
Msa0070260 MtrunA17_Chr7g0243081 56.604 53 23 0 181 233 73 125 4.48e-13 69.3
Msa0070260 MtrunA17_Chr6g0479431 50.847 59 29 0 181 239 204 262 6.69e-13 68.6
Msa0070260 MtrunA17_Chr1g0154261 47.368 76 36 2 181 255 128 200 8.45e-13 67.8
Msa0070260 MtrunA17_Chr3g0109981 38.532 109 51 3 181 289 276 368 1.06e-12 68.6
Msa0070260 MtrunA17_Chr2g0283951 50.000 74 25 2 181 250 91 156 2.38e-12 67.0
Msa0070260 MtrunA17_Chr5g0410741 48.333 60 31 0 181 240 221 280 4.50e-12 66.2
Msa0070260 MtrunA17_Chr2g0288601 57.143 49 21 0 181 229 73 121 5.57e-12 62.4
Msa0070260 MtrunA17_Chr7g0275401 34.127 126 54 2 138 235 123 247 2.87e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0070260 AT5G06800.1 42.941 170 94 1 140 309 156 322 9.01e-35 130
Msa0070260 AT5G06800.2 42.286 175 93 2 140 309 156 327 2.11e-34 129
Msa0070260 AT4G28610.1 46.528 144 72 2 166 307 215 355 9.62e-32 123
Msa0070260 AT2G01060.1 48.175 137 61 2 179 309 15 147 1.45e-30 117
Msa0070260 AT5G29000.1 43.165 139 75 2 175 309 184 322 3.43e-29 115
Msa0070260 AT5G29000.3 43.165 139 75 2 175 309 184 322 3.43e-29 115
Msa0070260 AT5G29000.2 43.165 139 75 2 175 309 227 365 3.80e-29 115
Msa0070260 AT3G04450.4 30.800 250 141 5 88 315 99 338 2.76e-28 113
Msa0070260 AT3G04450.2 30.800 250 141 5 88 315 99 338 2.76e-28 113
Msa0070260 AT3G04450.1 40.426 141 80 1 179 315 238 378 4.55e-28 113
Msa0070260 AT3G13040.3 41.096 146 70 2 175 309 237 377 3.31e-24 102
Msa0070260 AT3G13040.1 41.096 146 70 2 175 309 237 377 3.31e-24 102
Msa0070260 AT3G13040.2 41.096 146 70 2 175 309 237 377 3.31e-24 102
Msa0070260 AT4G13640.1 37.107 159 83 3 174 317 32 188 1.04e-23 99.0
Msa0070260 AT1G79430.2 38.854 157 84 2 168 316 23 175 1.69e-23 99.8
Msa0070260 AT5G18240.1 37.821 156 81 2 167 306 33 188 3.54e-23 99.4
Msa0070260 AT5G18240.4 37.821 156 81 2 167 306 33 188 3.54e-23 99.4
Msa0070260 AT2G20400.2 41.985 131 74 1 179 307 231 361 5.15e-23 98.6
Msa0070260 AT3G24120.1 39.490 157 82 2 174 317 36 192 9.38e-23 96.3
Msa0070260 AT3G04030.3 34.940 166 74 2 166 306 32 188 1.39e-22 97.4
Msa0070260 AT2G20400.1 42.748 131 72 2 179 307 231 360 1.48e-22 97.4
Msa0070260 AT4G13640.2 36.420 162 83 4 174 317 32 191 7.44e-22 94.0
Msa0070260 AT3G24120.2 38.750 160 82 3 174 317 36 195 8.93e-21 90.9
Msa0070260 AT5G18240.5 37.179 156 80 3 167 306 33 186 1.02e-20 92.4
Msa0070260 AT3G12730.1 35.135 148 87 2 168 306 12 159 2.29e-20 88.6
Msa0070260 AT3G04030.2 33.735 166 75 3 166 306 32 187 1.00e-19 89.4
Msa0070260 AT1G69580.1 36.913 149 76 2 174 304 29 177 3.80e-19 87.0
Msa0070260 AT5G18240.2 34.615 156 80 3 167 306 33 182 2.47e-18 85.5
Msa0070260 AT5G18240.3 34.615 156 80 3 167 306 33 182 2.47e-18 85.5
Msa0070260 AT1G69580.2 35.065 154 73 2 174 304 29 178 3.60e-18 84.3
Msa0070260 AT3G04030.1 31.928 166 73 3 166 306 32 182 9.34e-18 83.6
Msa0070260 AT5G45580.2 31.902 163 86 3 169 309 13 172 1.02e-17 82.0
Msa0070260 AT5G45580.1 32.278 158 82 3 174 309 2 156 2.12e-17 80.9
Msa0070260 AT5G29000.4 58.333 60 25 0 175 234 227 286 6.18e-17 80.5
Msa0070260 AT5G06800.3 45.161 93 48 1 140 232 156 245 3.12e-16 77.8
Msa0070260 AT3G04450.3 55.172 58 26 0 179 236 238 295 5.18e-16 77.8
Msa0070260 AT2G42660.1 58.491 53 22 0 181 233 52 104 1.41e-14 72.8
Msa0070260 AT4G04605.1 60.377 53 21 0 181 233 16 68 1.80e-14 72.0
Msa0070260 AT2G40260.1 60.377 53 21 0 181 233 84 136 2.83e-14 73.6
Msa0070260 AT5G42630.2 43.478 92 46 3 161 250 86 173 2.87e-14 71.2
Msa0070260 AT5G42630.1 43.478 92 46 3 161 250 86 173 3.19e-14 72.0
Msa0070260 AT5G16560.1 41.111 90 53 0 181 270 221 310 5.38e-14 72.4
Msa0070260 AT4G04580.1 58.491 53 22 0 181 233 16 68 5.51e-14 69.3
Msa0070260 AT1G14600.1 61.111 54 21 0 181 234 25 78 5.57e-14 71.2
Msa0070260 AT2G01060.2 45.098 102 46 2 214 309 1 98 6.12e-14 70.9
Msa0070260 AT2G38300.1 46.575 73 31 1 181 253 56 120 3.49e-13 69.7
Msa0070260 AT1G32240.2 37.143 105 60 2 181 279 215 319 7.89e-13 68.9
Msa0070260 AT1G32240.1 37.143 105 60 2 181 279 215 319 8.79e-13 68.9
Msa0070260 AT2G02060.2 58.491 53 22 0 181 233 31 83 9.36e-13 67.8
Msa0070260 AT2G02060.1 58.491 53 22 0 181 233 31 83 9.36e-13 67.8
Msa0070260 AT4G17695.1 50.000 70 32 2 173 239 155 224 1.18e-12 68.2
Msa0070260 AT2G06020.1 48.276 58 30 0 175 232 82 139 1.82e-12 67.4
Msa0070260 AT4G04555.1 58.824 51 21 0 183 233 18 68 3.66e-12 62.4
Msa0070260 AT4G37180.1 45.333 75 40 1 164 238 196 269 2.14e-11 64.7
Msa0070260 AT4G37180.2 45.333 75 40 1 164 238 203 276 2.53e-11 64.3

Find 53 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAACAACCTGCTTCAAAT+TGG 0.228288 1_2:-40340463 Msa0070260:CDS
AAAGGTGAATCTGTTGTTAT+TGG 0.269136 1_2:+40340543 None:intergenic
CAGCCTTGACAATACATAAA+AGG 0.269493 1_2:+40340525 None:intergenic
ATGAGTTTGACATACCTATT+TGG 0.292306 1_2:-40340584 Msa0070260:CDS
GTGATTCTTCTATTTGCATT+TGG 0.336254 1_2:+40338114 None:intergenic
TATCTTAAAATCAGAGTTGC+TGG 0.350060 1_2:+40339343 None:intergenic
TCATGTCAAAAGTCATTTGC+AGG 0.353917 1_2:-40339084 Msa0070260:intron
CTAAAATGTACATAAATCAT+TGG 0.360570 1_2:-40340734 None:intergenic
TTCCCTCTTGTTCTGATAAG+AGG 0.378026 1_2:-40340377 Msa0070260:CDS
CCCAATTTGAAGCAGGTTGT+TGG 0.384228 1_2:+40340462 None:intergenic
GTAAATTCATTGCTCCAAAT+AGG 0.396813 1_2:+40340570 None:intergenic
TAAAATGTACATAAATCATT+GGG 0.405097 1_2:-40340733 None:intergenic
CCAACAACCTGCTTCAAATT+GGG 0.416004 1_2:-40340462 Msa0070260:CDS
ATTTCATGGGACCTATGATC+TGG 0.435142 1_2:+40340333 None:intergenic
GACATATCAGCAATAATTCA+TGG 0.436755 1_2:-40340660 Msa0070260:CDS
ATTATATAAACTTACTTTCA+GGG 0.437925 1_2:+40339240 None:intergenic
ACAAAAGAAAAGAACCAAAC+AGG 0.442809 1_2:-40337722 Msa0070260:CDS
ATCATTTGTAGTATAGCCTT+AGG 0.450838 1_2:+40339131 None:intergenic
TAAGGCTATACTACAAATGA+TGG 0.452684 1_2:-40339129 Msa0070260:CDS
ATTATGATTGTTAACCAGCT+TGG 0.463406 1_2:-40339266 Msa0070260:CDS
TTGAGCTTGAAAATTCATAT+AGG 0.463644 1_2:+40340620 None:intergenic
TCCCTCTTGTTCTGATAAGA+GGG 0.481140 1_2:-40340376 Msa0070260:CDS
TTCAGCACCCAATTTGAAGC+AGG 0.483419 1_2:+40340455 None:intergenic
TACTTTCAGGGCCACCAAGC+TGG 0.490074 1_2:+40339252 None:intergenic
TTGTTCTGATAAGAGGGTGC+TGG 0.508794 1_2:-40340370 Msa0070260:CDS
TTTCATGGGACCTATGATCT+GGG 0.511209 1_2:+40340334 None:intergenic
GATGCAGCGAAATTTGCAAC+TGG 0.519384 1_2:-40337788 Msa0070260:CDS
ATGATTGTTAACCAGCTTGG+TGG 0.519893 1_2:-40339263 Msa0070260:CDS
CACACGCACAAATGTTTGAA+AGG 0.526179 1_2:-40338793 Msa0070260:intron
TTTCTGAAAGGAAATTTCAT+GGG 0.532722 1_2:+40340320 None:intergenic
GAGGAAGGACAGATAACAGA+TGG 0.535680 1_2:-40338298 Msa0070260:CDS
AGGGTAAAAGAAGATTAAGA+TGG 0.538823 1_2:-40339313 Msa0070260:CDS
AGAGGACTAGCTATGCTACA+AGG 0.548072 1_2:+40340687 None:intergenic
AATCACAGAGGCAAAGCCTA+AGG 0.564212 1_2:-40339147 Msa0070260:intron
GAGCAATCAACGACAATTCG+AGG 0.567446 1_2:-40338066 Msa0070260:intron
AACTACAACTTGAGGTTGAG+AGG 0.574473 1_2:-40338088 Msa0070260:CDS
CACCCTCTTATCAGAACAAG+AGG 0.577698 1_2:+40340374 None:intergenic
AATTTCCTTTCAGAAAACTC+AGG 0.594791 1_2:-40340313 Msa0070260:intron
GACAAACAATCTTCACCTTG+TGG 0.605075 1_2:-40339383 Msa0070260:CDS
AAGGCTATACTACAAATGAT+GGG 0.610409 1_2:-40339128 Msa0070260:CDS
CTGTGTTGCAGGAGTATCTG+AGG 0.610708 1_2:-40338317 Msa0070260:intron
ATTGCTGATATGTCCTCTAG+AGG 0.610808 1_2:+40340669 None:intergenic
AGGAAGGACAGATAACAGAT+GGG 0.621828 1_2:-40338297 Msa0070260:CDS
TGAATCTGTTGTTATTGGCA+TGG 0.626242 1_2:+40340548 None:intergenic
GAGGACTAGCTATGCTACAA+GGG 0.630241 1_2:+40340688 None:intergenic
TAGCATAGCTAGTCCTCTAG+AGG 0.637217 1_2:-40340682 Msa0070260:CDS
ATCACGACAACTACAACTTG+AGG 0.638258 1_2:-40338096 Msa0070260:CDS
TTCATGGGACCTATGATCTG+GGG 0.644180 1_2:+40340335 None:intergenic
TATACTACAAATGATGGGTG+TGG 0.647886 1_2:-40339123 Msa0070260:CDS
GTATGTCAAACTCATGCTGA+TGG 0.650055 1_2:+40340592 None:intergenic
GTTGCAGGAGTATCTGAGGA+AGG 0.655805 1_2:-40338313 Msa0070260:intron
GTTGCAAAATCAAAGCCACA+AGG 0.709667 1_2:+40339368 None:intergenic
ACCCTCTTATCAGAACAAGA+GGG 0.715933 1_2:+40340375 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATGAAACAATTAT+TGG + chr1_2:40338420-40338439 None:intergenic 10.0%
!!! TTTTTTTTTATCAGTAAATA+TGG + chr1_2:40340000-40340019 None:intergenic 10.0%
!!! ATATTTTATATCAAGAATTA+AGG - chr1_2:40340451-40340470 Msa0070260:CDS 10.0%
!! AAAGAGTCATATAAAAATTA+AGG + chr1_2:40338528-40338547 None:intergenic 15.0%
!!! ATTATTGATTACAATGAATT+TGG - chr1_2:40338576-40338595 Msa0070260:intron 15.0%
!!! TTATTGATTACAATGAATTT+GGG - chr1_2:40338577-40338596 Msa0070260:intron 15.0%
!! ATTATATAAACTTACTTTCA+GGG + chr1_2:40339185-40339204 None:intergenic 15.0%
!! AATTATATAAACTTACTTTC+AGG + chr1_2:40339186-40339205 None:intergenic 15.0%
!! TTATTAATTTATTAGCGATA+TGG - chr1_2:40339529-40339548 Msa0070260:intron 15.0%
!! TATTAATTTATTAGCGATAT+GGG - chr1_2:40339530-40339549 Msa0070260:intron 15.0%
!! TTGAAATTGAAAACTAAATT+TGG + chr1_2:40339729-40339748 None:intergenic 15.0%
!! AAATTAATTAAGAATAGAGA+AGG - chr1_2:40339874-40339893 Msa0070260:intron 15.0%
!! AATTAATTAAGAATAGAGAA+GGG - chr1_2:40339875-40339894 Msa0070260:intron 15.0%
!!! ATCTTAATCACATAATTTTA+AGG + chr1_2:40340197-40340216 None:intergenic 15.0%
!! TAACTTCTTCAAAAATATTA+AGG - chr1_2:40340569-40340588 Msa0070260:CDS 15.0%
!! AATTTGAAGAGTAATAAGAA+AGG + chr1_2:40338156-40338175 None:intergenic 20.0%
!!! ATTACTCTTCAAATTTTTGT+TGG - chr1_2:40338161-40338180 Msa0070260:intron 20.0%
!!! TAATAAAATGATACCCTTTT+AGG + chr1_2:40338631-40338650 None:intergenic 20.0%
!!! TCATCATACATATTTAAGAT+TGG + chr1_2:40338804-40338823 None:intergenic 20.0%
!! ATCTTAAATATGTATGATGA+CGG - chr1_2:40338804-40338823 Msa0070260:CDS 20.0%
!! TGAATATGAACAATTAAAGA+AGG + chr1_2:40340220-40340239 None:intergenic 20.0%
!! CTTTAATTGTTCATATTCAT+TGG - chr1_2:40340221-40340240 Msa0070260:intron 20.0%
!! ATTATTGACATCAAGAATTT+AGG - chr1_2:40340393-40340412 Msa0070260:CDS 20.0%
!! GAATTAAGGAAATATTATGT+TGG - chr1_2:40340465-40340484 Msa0070260:CDS 20.0%
! TTGAGCTTGAAAATTCATAT+AGG + chr1_2:40337805-40337824 None:intergenic 25.0%
!! TTTCTGAAAGGAAATTTCAT+GGG + chr1_2:40338105-40338124 None:intergenic 25.0%
!!! TTTTCTGAAAGGAAATTTCA+TGG + chr1_2:40338106-40338125 None:intergenic 25.0%
! AACCATTGTTAATTAGTCAA+TGG - chr1_2:40338188-40338207 Msa0070260:intron 25.0%
!! ATCCATTGACTAATTAACAA+TGG + chr1_2:40338193-40338212 None:intergenic 25.0%
! ATTAGTCAATGGATGAATTT+AGG - chr1_2:40338199-40338218 Msa0070260:intron 25.0%
! TAATTGACTACAGTTTCAAT+AGG - chr1_2:40338552-40338571 Msa0070260:intron 25.0%
! TTAGGTATACATATTGCTTT+GGG + chr1_2:40338613-40338632 None:intergenic 25.0%
! TTTAGGTATACATATTGCTT+TGG + chr1_2:40338614-40338633 None:intergenic 25.0%
! AAGCAATATGTATACCTAAA+AGG - chr1_2:40338614-40338633 Msa0070260:intron 25.0%
! AGCAATATGTATACCTAAAA+GGG - chr1_2:40338615-40338634 Msa0070260:intron 25.0%
!!! CGGTTCAATTAATTTTAAGT+CGG - chr1_2:40338773-40338792 Msa0070260:intron 25.0%
!!! GTAGTTTTTGAAGGAATAAA+AGG - chr1_2:40338902-40338921 Msa0070260:intron 25.0%
! AAACTTCTAAATGGATCATT+GGG + chr1_2:40338938-40338957 None:intergenic 25.0%
! GTTACAAAGAAACTTCTAAA+TGG + chr1_2:40338947-40338966 None:intergenic 25.0%
! ACTATGATAAGACATTTATG+AGG + chr1_2:40338999-40339018 None:intergenic 25.0%
! TCATAAATGTCTTATCATAG+TGG - chr1_2:40338998-40339017 Msa0070260:intron 25.0%
! CATAAATGTCTTATCATAGT+GGG - chr1_2:40338999-40339018 Msa0070260:intron 25.0%
!!! TGATTTTAAGATAACTGCAA+AGG - chr1_2:40339089-40339108 Msa0070260:CDS 25.0%
!!! GATTTTAAGATAACTGCAAA+GGG - chr1_2:40339090-40339109 Msa0070260:CDS 25.0%
! TTATACTTTACCCTCAAAAT+AGG + chr1_2:40339406-40339425 None:intergenic 25.0%
! TGTTAGTAGAAAAATGAACA+AGG + chr1_2:40339677-40339696 None:intergenic 25.0%
! GGTTAAAATGCATTTCATAA+AGG + chr1_2:40339708-40339727 None:intergenic 25.0%
! CAACACAGGTAAATTAATAT+TGG + chr1_2:40340093-40340112 None:intergenic 25.0%
GACATATCAGCAATAATTCA+TGG - chr1_2:40337762-40337781 Msa0070260:CDS 30.0%
ATGAGTTTGACATACCTATT+TGG - chr1_2:40337838-40337857 Msa0070260:intron 30.0%
GTAAATTCATTGCTCCAAAT+AGG + chr1_2:40337855-40337874 None:intergenic 30.0%
AAAGGTGAATCTGTTGTTAT+TGG + chr1_2:40337882-40337901 None:intergenic 30.0%
! TCACCTTTTATGTATTGTCA+AGG - chr1_2:40337894-40337913 Msa0070260:intron 30.0%
AATTTCCTTTCAGAAAACTC+AGG - chr1_2:40338109-40338128 Msa0070260:CDS 30.0%
ATTTAGGAACACATTTGTCA+CGG - chr1_2:40338215-40338234 Msa0070260:intron 30.0%
TATCATATATGTTCCGCATT+GGG + chr1_2:40338252-40338271 None:intergenic 30.0%
ATATCATATATGTTCCGCAT+TGG + chr1_2:40338253-40338272 None:intergenic 30.0%
!!! TTTTGTTTCCTTAACTTGTG+TGG - chr1_2:40338284-40338303 Msa0070260:CDS 30.0%
! GAAACAATTATTGGTTAGAG+AGG + chr1_2:40338411-40338430 None:intergenic 30.0%
! TTTGATTTCATTCGATAAGG+AGG + chr1_2:40338468-40338487 None:intergenic 30.0%
! GAGTTTGATTTCATTCGATA+AGG + chr1_2:40338471-40338490 None:intergenic 30.0%
!! TTGTTGGACATCTTTTAAAG+CGG - chr1_2:40338753-40338772 Msa0070260:intron 30.0%
!!! CTATCGTTTGATTTTTTCAG+CGG + chr1_2:40338837-40338856 None:intergenic 30.0%
GAAACTTCTAAATGGATCAT+TGG + chr1_2:40338939-40338958 None:intergenic 30.0%
TATCTTAAAATCAGAGTTGC+TGG + chr1_2:40339082-40339101 None:intergenic 30.0%
AGGGTAAAAGAAGATTAAGA+TGG - chr1_2:40339109-40339128 Msa0070260:CDS 30.0%
ATTATGATTGTTAACCAGCT+TGG - chr1_2:40339156-40339175 Msa0070260:intron 30.0%
GATTAAATTAAGCACTAGCT+AGG + chr1_2:40339248-40339267 None:intergenic 30.0%
TAATCATTGATCAATCACAG+AGG - chr1_2:40339263-40339282 Msa0070260:CDS 30.0%
ATCATTTGTAGTATAGCCTT+AGG + chr1_2:40339294-40339313 None:intergenic 30.0%
TAAGGCTATACTACAAATGA+TGG - chr1_2:40339293-40339312 Msa0070260:CDS 30.0%
AAGGCTATACTACAAATGAT+GGG - chr1_2:40339294-40339313 Msa0070260:CDS 30.0%
! ATGACTTTTGACATGAGAAA+TGG + chr1_2:40339334-40339353 None:intergenic 30.0%
! ATATGCATGTACCTATTTTG+AGG - chr1_2:40339392-40339411 Msa0070260:CDS 30.0%
!! TATGCATGTACCTATTTTGA+GGG - chr1_2:40339393-40339412 Msa0070260:CDS 30.0%
GTAATTCACTTTAATCTGTG+TGG - chr1_2:40339651-40339670 Msa0070260:intron 30.0%
CACAGGTAAATTAATATTGG+AGG + chr1_2:40340090-40340109 None:intergenic 30.0%
TATTAATTTACCTGTGTTGC+AGG - chr1_2:40340094-40340113 Msa0070260:intron 30.0%
GTGATTCTTCTATTTGCATT+TGG + chr1_2:40340311-40340330 None:intergenic 30.0%
TATTTCAGTGTCAATTTGAG+TGG - chr1_2:40340539-40340558 Msa0070260:CDS 30.0%
ACAAAAGAAAAGAACCAAAC+AGG - chr1_2:40340700-40340719 Msa0070260:CDS 30.0%
! TGAATCTGTTGTTATTGGCA+TGG + chr1_2:40337877-40337896 None:intergenic 35.0%
CAGCCTTGACAATACATAAA+AGG + chr1_2:40337900-40337919 None:intergenic 35.0%
! ATGATTTTTCCCCAGATCAT+AGG - chr1_2:40338078-40338097 Msa0070260:CDS 35.0%
! AATCACCTGAGTTTTCTGAA+AGG + chr1_2:40338117-40338136 None:intergenic 35.0%
AACTTCTACCACACAAGTTA+AGG + chr1_2:40338295-40338314 None:intergenic 35.0%
! AGCTCTGATGTTAGATATGT+CGG - chr1_2:40338371-40338390 Msa0070260:intron 35.0%
! CATATTGCTTTGGGTACAAA+TGG + chr1_2:40338604-40338623 None:intergenic 35.0%
! TTTAGAGCAATTTCAGCAGT+GGG + chr1_2:40338690-40338709 None:intergenic 35.0%
!!! TTTTAGAGCAATTTCAGCAG+TGG + chr1_2:40338691-40338710 None:intergenic 35.0%
AAACTATTCCAGCAATCGTT+CGG - chr1_2:40338708-40338727 Msa0070260:intron 35.0%
! GTGAGAAGAGTAGTTTTTGA+AGG - chr1_2:40338893-40338912 Msa0070260:intron 35.0%
!! TATACTACAAATGATGGGTG+TGG - chr1_2:40339299-40339318 Msa0070260:CDS 35.0%
TCATGTCAAAAGTCATTTGC+AGG - chr1_2:40339338-40339357 Msa0070260:CDS 35.0%
TATAAAGCACAAACACTCCT+CGG - chr1_2:40339421-40339440 Msa0070260:intron 35.0%
TAGAGAAGGGAAAATGTGAA+AGG - chr1_2:40339888-40339907 Msa0070260:intron 35.0%
TCTCGTGAGTATAGTTCAAT+TGG + chr1_2:40339972-40339991 None:intergenic 35.0%
AAAGGATGGAGTAGGAAATT+TGG + chr1_2:40340284-40340303 None:intergenic 35.0%
CTATTTGCATTTGGCTACAA+AGG + chr1_2:40340302-40340321 None:intergenic 35.0%
! GAAATATTATGTTGGCCTGA+TGG - chr1_2:40340473-40340492 Msa0070260:CDS 35.0%
ATTGCTGATATGTCCTCTAG+AGG + chr1_2:40337756-40337775 None:intergenic 40.0%
GTATGTCAAACTCATGCTGA+TGG + chr1_2:40337833-40337852 None:intergenic 40.0%
ACCAACAACCTGCTTCAAAT+TGG - chr1_2:40337959-40337978 Msa0070260:intron 40.0%
CCAACAACCTGCTTCAAATT+GGG - chr1_2:40337960-40337979 Msa0070260:intron 40.0%
TTCCCTCTTGTTCTGATAAG+AGG - chr1_2:40338045-40338064 Msa0070260:intron 40.0%
TCCCTCTTGTTCTGATAAGA+GGG - chr1_2:40338046-40338065 Msa0070260:intron 40.0%
ACCCTCTTATCAGAACAAGA+GGG + chr1_2:40338050-40338069 None:intergenic 40.0%
TTTCATGGGACCTATGATCT+GGG + chr1_2:40338091-40338110 None:intergenic 40.0%
ATTTCATGGGACCTATGATC+TGG + chr1_2:40338092-40338111 None:intergenic 40.0%
GGTATAGATGTATCCCAATG+CGG - chr1_2:40338236-40338255 Msa0070260:intron 40.0%
TTCATTCGATAAGGAGGTTG+AGG + chr1_2:40338462-40338481 None:intergenic 40.0%
! TTCTTTGTTTGTCGAAGTCG+CGG - chr1_2:40338494-40338513 Msa0070260:intron 40.0%
TAGCGGGAGTTAATTGTTGT+TGG - chr1_2:40338737-40338756 Msa0070260:intron 40.0%
GACAAACAATCTTCACCTTG+TGG - chr1_2:40339039-40339058 Msa0070260:intron 40.0%
GTTGCAAAATCAAAGCCACA+AGG + chr1_2:40339057-40339076 None:intergenic 40.0%
ATGATTGTTAACCAGCTTGG+TGG - chr1_2:40339159-40339178 Msa0070260:intron 40.0%
TATATGTGTCAGTGTCGTGT+CGG + chr1_2:40339488-40339507 None:intergenic 40.0%
CACACGCACAAATGTTTGAA+AGG - chr1_2:40339629-40339648 Msa0070260:intron 40.0%
GAAGGGAAAATGTGAAAGGA+AGG - chr1_2:40339892-40339911 Msa0070260:intron 40.0%
AAGGAAGGTTTGCAAAGTAG+AGG - chr1_2:40339907-40339926 Msa0070260:intron 40.0%
AGGAAGGACAGATAACAGAT+GGG - chr1_2:40340125-40340144 Msa0070260:intron 40.0%
TTGCATTTGGCTACAAAGGA+TGG + chr1_2:40340298-40340317 None:intergenic 40.0%
ATCACGACAACTACAACTTG+AGG - chr1_2:40340326-40340345 Msa0070260:CDS 40.0%
AACTACAACTTGAGGTTGAG+AGG - chr1_2:40340334-40340353 Msa0070260:CDS 40.0%
ATCAACGACAATTCGAGGTA+CGG - chr1_2:40340361-40340380 Msa0070260:CDS 40.0%
! TTATGTTGGCCTGATGGTAT+TGG - chr1_2:40340479-40340498 Msa0070260:CDS 40.0%
TGAAATATGAGACCTTGAGG+AGG + chr1_2:40340526-40340545 None:intergenic 40.0%
CACTGAAATATGAGACCTTG+AGG + chr1_2:40340529-40340548 None:intergenic 40.0%
!! ATCGGTCATTTTCTCCTGTT+TGG + chr1_2:40340717-40340736 None:intergenic 40.0%
!! GAGGACTAGCTATGCTACAA+GGG + chr1_2:40337737-40337756 None:intergenic 45.0%
!! AGAGGACTAGCTATGCTACA+AGG + chr1_2:40337738-40337757 None:intergenic 45.0%
TAGCATAGCTAGTCCTCTAG+AGG - chr1_2:40337740-40337759 Msa0070260:CDS 45.0%
! CCCAATTTGAAGCAGGTTGT+TGG + chr1_2:40337963-40337982 None:intergenic 45.0%
! TTCAGCACCCAATTTGAAGC+AGG + chr1_2:40337970-40337989 None:intergenic 45.0%
CACCCTCTTATCAGAACAAG+AGG + chr1_2:40338051-40338070 None:intergenic 45.0%
! TTGTTCTGATAAGAGGGTGC+TGG - chr1_2:40338052-40338071 Msa0070260:intron 45.0%
TTCATGGGACCTATGATCTG+GGG + chr1_2:40338090-40338109 None:intergenic 45.0%
TAGAAAAGCCGAACGATTGC+TGG + chr1_2:40338719-40338738 None:intergenic 45.0%
! CAATCGTTCGGCTTTTCTAG+CGG - chr1_2:40338720-40338739 Msa0070260:intron 45.0%
!! AATCGTTCGGCTTTTCTAGC+GGG - chr1_2:40338721-40338740 Msa0070260:intron 45.0%
AATCACAGAGGCAAAGCCTA+AGG - chr1_2:40339275-40339294 Msa0070260:CDS 45.0%
GATTAGACGTGTCCTGGTAT+CGG - chr1_2:40339443-40339462 Msa0070260:intron 45.0%
GAAGGTTTGCAAAGTAGAGG+AGG - chr1_2:40339910-40339929 Msa0070260:intron 45.0%
GAGGAAGGACAGATAACAGA+TGG - chr1_2:40340124-40340143 Msa0070260:intron 45.0%
TTGGCTACAAAGGATGGAGT+AGG + chr1_2:40340292-40340311 None:intergenic 45.0%
GAGCAATCAACGACAATTCG+AGG - chr1_2:40340356-40340375 Msa0070260:CDS 45.0%
GGTTTCAAGCCAATACCATC+AGG + chr1_2:40340491-40340510 None:intergenic 45.0%
GATGCAGCGAAATTTGCAAC+TGG - chr1_2:40340634-40340653 Msa0070260:CDS 45.0%
!! TATTATATCATAAAAATAAA+TGG - chr1_2:40338345-40338364 Msa0070260:intron 5.0%
TTTGTTTGTCGAAGTCGCGG+TGG - chr1_2:40338497-40338516 Msa0070260:intron 50.0%
TTGTTTGTCGAAGTCGCGGT+GGG - chr1_2:40338498-40338517 Msa0070260:intron 50.0%
TACGACATGTGTCCGATACC+AGG + chr1_2:40339458-40339477 None:intergenic 50.0%
CTCAGATACTCCTGCAACAC+AGG + chr1_2:40340107-40340126 None:intergenic 50.0%
!! CTGTGTTGCAGGAGTATCTG+AGG - chr1_2:40340105-40340124 Msa0070260:intron 50.0%
!! GTTGCAGGAGTATCTGAGGA+AGG - chr1_2:40340109-40340128 Msa0070260:intron 50.0%
TACTTTCAGGGCCACCAAGC+TGG + chr1_2:40339173-40339192 None:intergenic 55.0%
! TCCTCGGATTAGACGTGTCC+TGG - chr1_2:40339437-40339456 Msa0070260:intron 55.0%
ACCAGGACACGTCTAATCCG+AGG + chr1_2:40339441-40339460 None:intergenic 55.0%
! TTGAGGAGGAGCACACTCTC+AGG + chr1_2:40340512-40340531 None:intergenic 55.0%
! TGAGAGTGTGCTCCTCCTCA+AGG - chr1_2:40340511-40340530 Msa0070260:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 40337693 40340751 40337693 ID=Msa0070260;Name=Msa0070260
chr1_2 mRNA 40337693 40340751 40337693 ID=Msa0070260-mRNA-1;Parent=Msa0070260;Name=Msa0070260-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|7|0|322
chr1_2 exon 40340314 40340751 40340314 ID=Msa0070260-mRNA-1:exon:12356;Parent=Msa0070260-mRNA-1
chr1_2 exon 40339255 40339426 40339255 ID=Msa0070260-mRNA-1:exon:12355;Parent=Msa0070260-mRNA-1
chr1_2 exon 40339085 40339161 40339085 ID=Msa0070260-mRNA-1:exon:12354;Parent=Msa0070260-mRNA-1
chr1_2 exon 40338794 40338833 40338794 ID=Msa0070260-mRNA-1:exon:12353;Parent=Msa0070260-mRNA-1
chr1_2 exon 40338274 40338328 40338274 ID=Msa0070260-mRNA-1:exon:12352;Parent=Msa0070260-mRNA-1
chr1_2 exon 40338067 40338136 40338067 ID=Msa0070260-mRNA-1:exon:12351;Parent=Msa0070260-mRNA-1
chr1_2 exon 40337693 40337809 40337693 ID=Msa0070260-mRNA-1:exon:12350;Parent=Msa0070260-mRNA-1
chr1_2 CDS 40340314 40340751 40340314 ID=Msa0070260-mRNA-1:cds;Parent=Msa0070260-mRNA-1
chr1_2 CDS 40339255 40339426 40339255 ID=Msa0070260-mRNA-1:cds;Parent=Msa0070260-mRNA-1
chr1_2 CDS 40339085 40339161 40339085 ID=Msa0070260-mRNA-1:cds;Parent=Msa0070260-mRNA-1
chr1_2 CDS 40338794 40338833 40338794 ID=Msa0070260-mRNA-1:cds;Parent=Msa0070260-mRNA-1
chr1_2 CDS 40338274 40338328 40338274 ID=Msa0070260-mRNA-1:cds;Parent=Msa0070260-mRNA-1
chr1_2 CDS 40338067 40338136 40338067 ID=Msa0070260-mRNA-1:cds;Parent=Msa0070260-mRNA-1
chr1_2 CDS 40337693 40337809 40337693 ID=Msa0070260-mRNA-1:cds;Parent=Msa0070260-mRNA-1
Gene Sequence

>Msa0070260

ATGTACATAAATCATTGGGATTATGAACATCAAAGCTATGACCCTTGTAGCATAGCTAGTCCTCTAGAGGACATATCAGCAATAATTCATGGATCAAATGTAAATGAAACCTATATGAATTTTCAAGCTCAACAACACCATCAGCATGAGTTTGACATACCTATTTGGAGCAATGAATTTACCATGCCAATAACAACAGATTCACCTTTTATGTATTGTCAAGGCTGTAAAGATAACTACTTAGAAAATATCACAAAACAAGATCAACCAACAACCTGCTTCAAATTGGGTGCTGAATCTTTAATGTCTAGCAGTGAAGATAGTATTTCTTCTTGTGAAAAGTGTAGTGAGTTTCCCTCTTGTTCTGATAAGAGGGTGCTGGAGAATGATTTTTCCCCAGATCATAGGTCCCATGAAATTTCCTTTCAGAAAACTCAGTGGGAATCATGTACAAAGCAAGACAAACAATCTTCACCTTGTGGCTTTGATTTTGCAACATCCAGCAACTCTGATTTTAAGATAACTGCAAAGGGTAAAAGAAGATTAAGATGGACAAAAGAATTGAATGAGTCATTTATTATGATTGTTAACCAGCTTGGTGGCCCTGAAAAGGCAAAGCCTAAGGCTATACTACAAATGATGGGTGTGGATGTGTTGACCATTTCTCATGTCAAAAGTCATTTGCAGAAATATCGTTCTACATTACACACGCACAAATGTTTGAAAGGAGTATCTGAGGAAGGACAGATAACAGATGGGATTAATGAGCTTCAAGTTAAAATCCAAATGCAAATAGAAGAATCACGACAACTACAACTTGAGGTTGAGAGGAGCAATCAACGACAATTCGAGATGCAGCGAAATTTGCAACTGGTGATTGAACAGCAGAAGAAGCAGCTCAAGTTAATGTTAGATCAACAAAAGAAAAGAACCAAACAGGAGAAAATGACCGATTTAAGACGAAAATGA
Protein sequence

>Msa0070260

MYINHWDYEHQSYDPCSIASPLEDISAIIHGSNVNETYMNFQAQQHHQHEFDIPIWSNEFTMPITTDSPFMYCQGCKDNYLENITKQDQPTTCFKLGAESLMSSSEDSISSCEKCSEFPSCSDKRVLENDFSPDHRSHEISFQKTQWESCTKQDKQSSPCGFDFATSSNSDFKITAKGKRRLRWTKELNESFIMIVNQLGGPEKAKPKAILQMMGVDVLTISHVKSHLQKYRSTLHTHKCLKGVSEEGQITDGINELQVKIQMQIEESRQLQLEVERSNQRQFEMQRNLQLVIEQQKKQLKLMLDQQKKRTKQEKMTDLRRK*