AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0072230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0072230 MtrunA17_Chr1g0179061 94.628 242 10 2 1 241 1 240 4.81e-168 466
Msa0072230 MtrunA17_Chr2g0311101 81.677 322 25 10 1 312 13 310 3.28e-163 456
Msa0072230 MtrunA17_Chr8g0384141 58.491 53 22 0 182 234 125 177 2.04e-13 68.9
Msa0072230 MtrunA17_Chr3g0134001 58.491 53 22 0 182 234 182 234 1.33e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0072230 AT1G30670.1 41.520 171 97 2 135 305 95 262 3.07e-29 113
Msa0072230 AT2G34820.1 42.254 213 115 4 97 305 81 289 2.91e-28 110
Msa0072230 AT3G21330.1 60.377 53 21 0 182 234 280 332 2.16e-13 70.5
Msa0072230 AT5G01305.1 50.000 58 29 0 182 239 48 105 1.37e-12 65.5
Msa0072230 AT4G00120.1 52.830 53 25 0 182 234 123 175 6.64e-12 63.9

Find 54 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTGGAATGCTTCAACTTA+TGG 0.195386 1_2:-44911113 Msa0072230:CDS
ATCTCTGCTGTGTTCATCTT+TGG 0.238213 1_2:+44911178 None:intergenic
TTGGTGAAATCTTCCTCATT+TGG 0.290806 1_2:+44911499 None:intergenic
ATCAAAACCTACCATTCTTA+AGG 0.300143 1_2:-44910067 Msa0072230:CDS
TCAAATAGTTAGAGGGAAAA+TGG 0.318116 1_2:+44911674 None:intergenic
AAATAAAACTATTCTTGCTT+TGG 0.325467 1_2:+44910006 None:intergenic
TGGTGAAATCTTCCTCATTT+GGG 0.332786 1_2:+44911500 None:intergenic
GAAGCAAAAGGGTTAGGGTT+AGG 0.345524 1_2:+44911364 None:intergenic
AAGCAAAAGGGTTAGGGTTA+GGG 0.345934 1_2:+44911365 None:intergenic
TCTCCTCGTAGCATAGCTTT+TGG 0.368098 1_2:+44911455 None:intergenic
AGTTGATCTTGACAAGGGTT+AGG 0.369040 1_2:+44911583 None:intergenic
TCAAAGGAAGCAAAAGGGTT+AGG 0.369513 1_2:+44911358 None:intergenic
AGTAGTGATTTCTAAGTTGT+TGG 0.372241 1_2:+44911738 None:intergenic
GTAGTTTGAATAGGTTGATA+AGG 0.378786 1_2:+44911760 None:intergenic
TACAGTTTCTCTTGAACAAA+TGG 0.383908 1_2:+44910156 None:intergenic
AAGGGTCTGAAACAGCTTGC+TGG 0.388228 1_2:-44910048 Msa0072230:CDS
AATGTGGTGACAAACTCTTT+TGG 0.390869 1_2:+44910111 None:intergenic
GAGCTTGGGAAGTTGGTTCC+TGG 0.392352 1_2:-44911205 Msa0072230:CDS
GACACAAGAGCTTGGGAAGT+TGG 0.397999 1_2:-44911212 Msa0072230:CDS
AAGTTGTTGGTAGTTTGAAT+AGG 0.402079 1_2:+44911751 None:intergenic
TTGATTATTTCAGGAAGAAA+AGG 0.406675 1_2:-44910220 Msa0072230:intron
TAGTTTGAATAGGTTGATAA+GGG 0.429054 1_2:+44911761 None:intergenic
AGTTTCATTCAAATAGTTAG+AGG 0.435549 1_2:+44911666 None:intergenic
GTTGATCTTGACAAGGGTTA+GGG 0.453473 1_2:+44911584 None:intergenic
TCAAAACCTACCATTCTTAA+GGG 0.456430 1_2:-44910066 Msa0072230:CDS
CAAAGGAAGCAAAAGGGTTA+GGG 0.461232 1_2:+44911359 None:intergenic
AGAGTATCGCGGCGAGAGAA+AGG 0.466876 1_2:-44911255 Msa0072230:CDS
TCTACCTCAAAGGAAGCAAA+AGG 0.468900 1_2:+44911352 None:intergenic
TTGTGTAGATCTTCACTTGG+AGG 0.473071 1_2:+44910192 None:intergenic
GTTCAATGTGAGAAGAAAGT+TGG 0.488552 1_2:-44911298 Msa0072230:CDS
TGTAGATCTTCACTTGGAGG+AGG 0.494574 1_2:+44910195 None:intergenic
GTGTTCATCTTTGGTCCACC+AGG 0.499817 1_2:+44911187 None:intergenic
ACTGAGAAGACACAAGAGCT+TGG 0.499993 1_2:-44911220 Msa0072230:CDS
CTGAGAAGACACAAGAGCTT+GGG 0.500383 1_2:-44911219 Msa0072230:CDS
CTGTTTCAGACCCTTAAGAA+TGG 0.516519 1_2:+44910056 None:intergenic
AGGGTCTGAAACAGCTTGCT+GGG 0.522554 1_2:-44910047 Msa0072230:CDS
GTTTCATTCAAATAGTTAGA+GGG 0.549802 1_2:+44911667 None:intergenic
CTTGGGAAGTTGGTTCCTGG+TGG 0.551828 1_2:-44911202 Msa0072230:CDS
GTGAGAAGAAAGTTGGTGAA+AGG 0.566992 1_2:-44911291 Msa0072230:CDS
AAGTTTCTTCAAGCTCAAGT+TGG 0.567049 1_2:-44911130 Msa0072230:CDS
AGCAAAGGGTTCTACCTCAA+AGG 0.572532 1_2:+44911342 None:intergenic
AGTTTGTGTAGATCTTCACT+TGG 0.582386 1_2:+44910189 None:intergenic
TTCAGACCCTTAAGAATGGT+AGG 0.583734 1_2:+44910060 None:intergenic
GAGTTCAAAAGCTCTTGTTG+TGG 0.586358 1_2:+44911421 None:intergenic
TCGCGGCGAGAGAAAGGAGA+AGG 0.587504 1_2:-44911249 Msa0072230:CDS
ACTTATGGAAACATTTAACA+AGG 0.595324 1_2:-44911098 Msa0072230:intron
CTACCTCAAAGGAAGCAAAA+GGG 0.620756 1_2:+44911353 None:intergenic
AATAGTTAGAGGGAAAATGG+TGG 0.625963 1_2:+44911677 None:intergenic
ACAAGTAGTTGATCTTGACA+AGG 0.633389 1_2:+44911577 None:intergenic
CAAGTAGTTGATCTTGACAA+GGG 0.656926 1_2:+44911578 None:intergenic
ATCATTATGATTGCTCAATG+TGG 0.666694 1_2:+44910095 None:intergenic
ATGCTACGAGGAGAAAAGAG+AGG 0.681365 1_2:-44911446 Msa0072230:CDS
TATCTCGTCGCAGAGTATCG+CGG 0.686396 1_2:-44911266 Msa0072230:CDS
ACGCCAAAAGCTATGCTACG+AGG 0.715032 1_2:-44911458 Msa0072230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTTGTAAATATCTAAA+TGG - chr1_2:44911091-44911110 Msa0072230:intron 15.0%
!! TATTGTTGTAAATATCTAAA+TGG - chr1_2:44911091-44911110 Msa0072230:intron 15.0%
!! TTTCATTTGAGTAAAAACAA+GGG + chr1_2:44910205-44910224 None:intergenic 20.0%
!! CGTTTGATAAAAAAATATAG+AGG - chr1_2:44911035-44911054 Msa0072230:intron 20.0%
!! CATAATAACACTACAATAAA+TGG + chr1_2:44911066-44911085 None:intergenic 20.0%
!! TTTACTCCATGAAAATATTT+AGG - chr1_2:44911226-44911245 Msa0072230:CDS 20.0%
!!! ACAATTCCTAAATATTTTCA+TGG + chr1_2:44911235-44911254 None:intergenic 20.0%
!! GGAATTGTTATAAAAATTGA+CGG - chr1_2:44911247-44911266 Msa0072230:CDS 20.0%
!!! TTTTGCTAATTGATTATTTC+AGG - chr1_2:44911584-44911603 Msa0072230:CDS 20.0%
!! TTTCATTTGAGTAAAAACAA+GGG + chr1_2:44910205-44910224 None:intergenic 20.0%
!! CGTTTGATAAAAAAATATAG+AGG - chr1_2:44911035-44911054 Msa0072230:intron 20.0%
!! CATAATAACACTACAATAAA+TGG + chr1_2:44911066-44911085 None:intergenic 20.0%
!! TTTACTCCATGAAAATATTT+AGG - chr1_2:44911226-44911245 Msa0072230:CDS 20.0%
!!! ACAATTCCTAAATATTTTCA+TGG + chr1_2:44911235-44911254 None:intergenic 20.0%
!! GGAATTGTTATAAAAATTGA+CGG - chr1_2:44911247-44911266 Msa0072230:CDS 20.0%
!!! TTTTGCTAATTGATTATTTC+AGG - chr1_2:44911584-44911603 Msa0072230:CDS 20.0%
!!! TAGTTTGAATAGGTTGATAA+GGG + chr1_2:44910055-44910074 None:intergenic 25.0%
! GTTTCATTCAAATAGTTAGA+GGG + chr1_2:44910149-44910168 None:intergenic 25.0%
! AGTTTCATTCAAATAGTTAG+AGG + chr1_2:44910150-44910169 None:intergenic 25.0%
! TTCATTTGAGTAAAAACAAG+GGG + chr1_2:44910204-44910223 None:intergenic 25.0%
! GTTTCATTTGAGTAAAAACA+AGG + chr1_2:44910206-44910225 None:intergenic 25.0%
!!! ATCATTTTGAGAAGAAAAAG+TGG + chr1_2:44910267-44910286 None:intergenic 25.0%
!!! ACTTTTTCTTCTCAAAATGA+TGG - chr1_2:44910266-44910285 Msa0072230:intron 25.0%
! AAACTCATCCACAAAAAAAT+TGG + chr1_2:44910420-44910439 None:intergenic 25.0%
! ACTTATGGAAACATTTAACA+AGG - chr1_2:44910715-44910734 Msa0072230:intron 25.0%
!! TTAAACTGTTTTGTGTACTT+AGG - chr1_2:44910827-44910846 Msa0072230:intron 25.0%
! AGAAAAACATCAAATTCACT+TGG + chr1_2:44910867-44910886 None:intergenic 25.0%
! TTGTTTGTTCATTGCATATT+TGG - chr1_2:44910967-44910986 Msa0072230:intron 25.0%
! AAAGACAAAATTGAGACAAT+AGG + chr1_2:44911179-44911198 None:intergenic 25.0%
!! TATTTTCATGGAGTAAAAGT+TGG + chr1_2:44911223-44911242 None:intergenic 25.0%
! TTGTTATAAAAATTGACGGA+TGG - chr1_2:44911251-44911270 Msa0072230:CDS 25.0%
! TGTTAGGTTTAAGAAAATGT+TGG - chr1_2:44911277-44911296 Msa0072230:CDS 25.0%
! TAACTGTTTAACAAGGAATT+TGG - chr1_2:44911445-44911464 Msa0072230:CDS 25.0%
! TTGATTATTTCAGGAAGAAA+AGG - chr1_2:44911593-44911612 Msa0072230:CDS 25.0%
!!! TAGTTTGAATAGGTTGATAA+GGG + chr1_2:44910055-44910074 None:intergenic 25.0%
! GTTTCATTCAAATAGTTAGA+GGG + chr1_2:44910149-44910168 None:intergenic 25.0%
! AGTTTCATTCAAATAGTTAG+AGG + chr1_2:44910150-44910169 None:intergenic 25.0%
! TTCATTTGAGTAAAAACAAG+GGG + chr1_2:44910204-44910223 None:intergenic 25.0%
! GTTTCATTTGAGTAAAAACA+AGG + chr1_2:44910206-44910225 None:intergenic 25.0%
!!! ATCATTTTGAGAAGAAAAAG+TGG + chr1_2:44910267-44910286 None:intergenic 25.0%
!!! ACTTTTTCTTCTCAAAATGA+TGG - chr1_2:44910266-44910285 Msa0072230:intron 25.0%
! AAACTCATCCACAAAAAAAT+TGG + chr1_2:44910420-44910439 None:intergenic 25.0%
! ACTTATGGAAACATTTAACA+AGG - chr1_2:44910715-44910734 Msa0072230:intron 25.0%
!! TTAAACTGTTTTGTGTACTT+AGG - chr1_2:44910827-44910846 Msa0072230:intron 25.0%
! AGAAAAACATCAAATTCACT+TGG + chr1_2:44910867-44910886 None:intergenic 25.0%
! TTGTTTGTTCATTGCATATT+TGG - chr1_2:44910967-44910986 Msa0072230:intron 25.0%
! AAAGACAAAATTGAGACAAT+AGG + chr1_2:44911179-44911198 None:intergenic 25.0%
!! TATTTTCATGGAGTAAAAGT+TGG + chr1_2:44911223-44911242 None:intergenic 25.0%
! TTGTTATAAAAATTGACGGA+TGG - chr1_2:44911251-44911270 Msa0072230:CDS 25.0%
! TGTTAGGTTTAAGAAAATGT+TGG - chr1_2:44911277-44911296 Msa0072230:CDS 25.0%
! TAACTGTTTAACAAGGAATT+TGG - chr1_2:44911445-44911464 Msa0072230:CDS 25.0%
! TTGATTATTTCAGGAAGAAA+AGG - chr1_2:44911593-44911612 Msa0072230:CDS 25.0%
!! GTAGTTTGAATAGGTTGATA+AGG + chr1_2:44910056-44910075 None:intergenic 30.0%
!! AAGTTGTTGGTAGTTTGAAT+AGG + chr1_2:44910065-44910084 None:intergenic 30.0%
AGTAGTGATTTCTAAGTTGT+TGG + chr1_2:44910078-44910097 None:intergenic 30.0%
TCAAATAGTTAGAGGGAAAA+TGG + chr1_2:44910142-44910161 None:intergenic 30.0%
! GAATGAAATTTTCCCAAATG+AGG - chr1_2:44910301-44910320 Msa0072230:intron 30.0%
! TTTTGGACATGTAAGGTAAT+TGG + chr1_2:44910336-44910355 None:intergenic 30.0%
!!! GAACTCAACCAATTTTTTTG+TGG - chr1_2:44910409-44910428 Msa0072230:intron 30.0%
!!! ACAATTTTTGAAGCAGCAAA+GGG + chr1_2:44910488-44910507 None:intergenic 30.0%
!!! AACAATTTTTGAAGCAGCAA+AGG + chr1_2:44910489-44910508 None:intergenic 30.0%
ACTCAAATGATTACAAGCAA+GGG + chr1_2:44911009-44911028 None:intergenic 30.0%
TACTCAAATGATTACAAGCA+AGG + chr1_2:44911010-44911029 None:intergenic 30.0%
TAATGCTGTTGTTGTAGATT+TGG - chr1_2:44911118-44911137 Msa0072230:CDS 30.0%
!! CAATAAACGAGAGAGTATTT+AGG - chr1_2:44911389-44911408 Msa0072230:CDS 30.0%
TACAGTTTCTCTTGAACAAA+TGG + chr1_2:44911660-44911679 None:intergenic 30.0%
ATCATTATGATTGCTCAATG+TGG + chr1_2:44911721-44911740 None:intergenic 30.0%
ATCAAAACCTACCATTCTTA+AGG - chr1_2:44911746-44911765 Msa0072230:CDS 30.0%
TCAAAACCTACCATTCTTAA+GGG - chr1_2:44911747-44911766 Msa0072230:CDS 30.0%
!! GTAGTTTGAATAGGTTGATA+AGG + chr1_2:44910056-44910075 None:intergenic 30.0%
!! AAGTTGTTGGTAGTTTGAAT+AGG + chr1_2:44910065-44910084 None:intergenic 30.0%
AGTAGTGATTTCTAAGTTGT+TGG + chr1_2:44910078-44910097 None:intergenic 30.0%
TCAAATAGTTAGAGGGAAAA+TGG + chr1_2:44910142-44910161 None:intergenic 30.0%
! GAATGAAATTTTCCCAAATG+AGG - chr1_2:44910301-44910320 Msa0072230:intron 30.0%
! TTTTGGACATGTAAGGTAAT+TGG + chr1_2:44910336-44910355 None:intergenic 30.0%
!!! GAACTCAACCAATTTTTTTG+TGG - chr1_2:44910409-44910428 Msa0072230:intron 30.0%
!!! ACAATTTTTGAAGCAGCAAA+GGG + chr1_2:44910488-44910507 None:intergenic 30.0%
!!! AACAATTTTTGAAGCAGCAA+AGG + chr1_2:44910489-44910508 None:intergenic 30.0%
ACTCAAATGATTACAAGCAA+GGG + chr1_2:44911009-44911028 None:intergenic 30.0%
TACTCAAATGATTACAAGCA+AGG + chr1_2:44911010-44911029 None:intergenic 30.0%
TAATGCTGTTGTTGTAGATT+TGG - chr1_2:44911118-44911137 Msa0072230:CDS 30.0%
!! CAATAAACGAGAGAGTATTT+AGG - chr1_2:44911389-44911408 Msa0072230:CDS 30.0%
TACAGTTTCTCTTGAACAAA+TGG + chr1_2:44911660-44911679 None:intergenic 30.0%
ATCATTATGATTGCTCAATG+TGG + chr1_2:44911721-44911740 None:intergenic 30.0%
ATCAAAACCTACCATTCTTA+AGG - chr1_2:44911746-44911765 Msa0072230:CDS 30.0%
TCAAAACCTACCATTCTTAA+GGG - chr1_2:44911747-44911766 Msa0072230:CDS 30.0%
AATAGTTAGAGGGAAAATGG+TGG + chr1_2:44910139-44910158 None:intergenic 35.0%
CAAGTAGTTGATCTTGACAA+GGG + chr1_2:44910238-44910257 None:intergenic 35.0%
ACAAGTAGTTGATCTTGACA+AGG + chr1_2:44910239-44910258 None:intergenic 35.0%
TGGTGAAATCTTCCTCATTT+GGG + chr1_2:44910316-44910335 None:intergenic 35.0%
TTGGTGAAATCTTCCTCATT+TGG + chr1_2:44910317-44910336 None:intergenic 35.0%
GTTCAATGTGAGAAGAAAGT+TGG - chr1_2:44910515-44910534 Msa0072230:intron 35.0%
AAGTTTCTTCAAGCTCAAGT+TGG - chr1_2:44910683-44910702 Msa0072230:intron 35.0%
AGTTGGAATGCTTCAACTTA+TGG - chr1_2:44910700-44910719 Msa0072230:intron 35.0%
GCAATGAACAAACAAACTCT+AGG + chr1_2:44910962-44910981 None:intergenic 35.0%
ATTGAGACAATAGGTAGTCA+TGG + chr1_2:44911170-44911189 None:intergenic 35.0%
! AATTGACGGATGGAATTGTT+AGG - chr1_2:44911261-44911280 Msa0072230:CDS 35.0%
AGGAAACGGTTAATAAGAAG+AGG - chr1_2:44911359-44911378 Msa0072230:CDS 35.0%
CGGTTAATAAGAAGAGGAAA+TGG - chr1_2:44911365-44911384 Msa0072230:CDS 35.0%
CAAGTGTATATCAAATGAGC+TGG - chr1_2:44911417-44911436 Msa0072230:CDS 35.0%
GGCTATGTAACTGTTTAACA+AGG - chr1_2:44911438-44911457 Msa0072230:CDS 35.0%
AGTTTGTGTAGATCTTCACT+TGG + chr1_2:44911627-44911646 None:intergenic 35.0%
! AATGTGGTGACAAACTCTTT+TGG + chr1_2:44911705-44911724 None:intergenic 35.0%
AATAGTTAGAGGGAAAATGG+TGG + chr1_2:44910139-44910158 None:intergenic 35.0%
CAAGTAGTTGATCTTGACAA+GGG + chr1_2:44910238-44910257 None:intergenic 35.0%
ACAAGTAGTTGATCTTGACA+AGG + chr1_2:44910239-44910258 None:intergenic 35.0%
TGGTGAAATCTTCCTCATTT+GGG + chr1_2:44910316-44910335 None:intergenic 35.0%
TTGGTGAAATCTTCCTCATT+TGG + chr1_2:44910317-44910336 None:intergenic 35.0%
GTTCAATGTGAGAAGAAAGT+TGG - chr1_2:44910515-44910534 Msa0072230:intron 35.0%
AAGTTTCTTCAAGCTCAAGT+TGG - chr1_2:44910683-44910702 Msa0072230:intron 35.0%
AGTTGGAATGCTTCAACTTA+TGG - chr1_2:44910700-44910719 Msa0072230:intron 35.0%
GCAATGAACAAACAAACTCT+AGG + chr1_2:44910962-44910981 None:intergenic 35.0%
ATTGAGACAATAGGTAGTCA+TGG + chr1_2:44911170-44911189 None:intergenic 35.0%
! AATTGACGGATGGAATTGTT+AGG - chr1_2:44911261-44911280 Msa0072230:CDS 35.0%
AGGAAACGGTTAATAAGAAG+AGG - chr1_2:44911359-44911378 Msa0072230:CDS 35.0%
CGGTTAATAAGAAGAGGAAA+TGG - chr1_2:44911365-44911384 Msa0072230:CDS 35.0%
CAAGTGTATATCAAATGAGC+TGG - chr1_2:44911417-44911436 Msa0072230:CDS 35.0%
GGCTATGTAACTGTTTAACA+AGG - chr1_2:44911438-44911457 Msa0072230:CDS 35.0%
AGTTTGTGTAGATCTTCACT+TGG + chr1_2:44911627-44911646 None:intergenic 35.0%
! AATGTGGTGACAAACTCTTT+TGG + chr1_2:44911705-44911724 None:intergenic 35.0%
GTTGATCTTGACAAGGGTTA+GGG + chr1_2:44910232-44910251 None:intergenic 40.0%
AGTTGATCTTGACAAGGGTT+AGG + chr1_2:44910233-44910252 None:intergenic 40.0%
!! TTTGGCGTTTTGGACATGTA+AGG + chr1_2:44910343-44910362 None:intergenic 40.0%
! TAGCATAGCTTTTGGCGTTT+TGG + chr1_2:44910353-44910372 None:intergenic 40.0%
GAGTTCAAAAGCTCTTGTTG+TGG + chr1_2:44910395-44910414 None:intergenic 40.0%
AAGCAAAAGGGTTAGGGTTA+GGG + chr1_2:44910451-44910470 None:intergenic 40.0%
CAAAGGAAGCAAAAGGGTTA+GGG + chr1_2:44910457-44910476 None:intergenic 40.0%
TCAAAGGAAGCAAAAGGGTT+AGG + chr1_2:44910458-44910477 None:intergenic 40.0%
! TAACCCTTTTGCTTCCTTTG+AGG - chr1_2:44910457-44910476 Msa0072230:intron 40.0%
CTACCTCAAAGGAAGCAAAA+GGG + chr1_2:44910463-44910482 None:intergenic 40.0%
TCTACCTCAAAGGAAGCAAA+AGG + chr1_2:44910464-44910483 None:intergenic 40.0%
!! GTGAGAAGAAAGTTGGTGAA+AGG - chr1_2:44910522-44910541 Msa0072230:intron 40.0%
ATCTCTGCTGTGTTCATCTT+TGG + chr1_2:44910638-44910657 None:intergenic 40.0%
GAGTGTGTGTGTTAAGGAAA+CGG - chr1_2:44911345-44911364 Msa0072230:CDS 40.0%
TTGTGTAGATCTTCACTTGG+AGG + chr1_2:44911624-44911643 None:intergenic 40.0%
TTCAGACCCTTAAGAATGGT+AGG + chr1_2:44911756-44911775 None:intergenic 40.0%
CTGTTTCAGACCCTTAAGAA+TGG + chr1_2:44911760-44911779 None:intergenic 40.0%
GTTGATCTTGACAAGGGTTA+GGG + chr1_2:44910232-44910251 None:intergenic 40.0%
AGTTGATCTTGACAAGGGTT+AGG + chr1_2:44910233-44910252 None:intergenic 40.0%
!! TTTGGCGTTTTGGACATGTA+AGG + chr1_2:44910343-44910362 None:intergenic 40.0%
! TAGCATAGCTTTTGGCGTTT+TGG + chr1_2:44910353-44910372 None:intergenic 40.0%
GAGTTCAAAAGCTCTTGTTG+TGG + chr1_2:44910395-44910414 None:intergenic 40.0%
AAGCAAAAGGGTTAGGGTTA+GGG + chr1_2:44910451-44910470 None:intergenic 40.0%
CAAAGGAAGCAAAAGGGTTA+GGG + chr1_2:44910457-44910476 None:intergenic 40.0%
TCAAAGGAAGCAAAAGGGTT+AGG + chr1_2:44910458-44910477 None:intergenic 40.0%
! TAACCCTTTTGCTTCCTTTG+AGG - chr1_2:44910457-44910476 Msa0072230:intron 40.0%
CTACCTCAAAGGAAGCAAAA+GGG + chr1_2:44910463-44910482 None:intergenic 40.0%
TCTACCTCAAAGGAAGCAAA+AGG + chr1_2:44910464-44910483 None:intergenic 40.0%
!! GTGAGAAGAAAGTTGGTGAA+AGG - chr1_2:44910522-44910541 Msa0072230:intron 40.0%
ATCTCTGCTGTGTTCATCTT+TGG + chr1_2:44910638-44910657 None:intergenic 40.0%
GAGTGTGTGTGTTAAGGAAA+CGG - chr1_2:44911345-44911364 Msa0072230:CDS 40.0%
TTGTGTAGATCTTCACTTGG+AGG + chr1_2:44911624-44911643 None:intergenic 40.0%
TTCAGACCCTTAAGAATGGT+AGG + chr1_2:44911756-44911775 None:intergenic 40.0%
CTGTTTCAGACCCTTAAGAA+TGG + chr1_2:44911760-44911779 None:intergenic 40.0%
! TCTCCTCGTAGCATAGCTTT+TGG + chr1_2:44910361-44910380 None:intergenic 45.0%
ATGCTACGAGGAGAAAAGAG+AGG - chr1_2:44910367-44910386 Msa0072230:intron 45.0%
GAAGCAAAAGGGTTAGGGTT+AGG + chr1_2:44910452-44910471 None:intergenic 45.0%
AGCAAAGGGTTCTACCTCAA+AGG + chr1_2:44910474-44910493 None:intergenic 45.0%
ACTGAGAAGACACAAGAGCT+TGG - chr1_2:44910593-44910612 Msa0072230:intron 45.0%
CTGAGAAGACACAAGAGCTT+GGG - chr1_2:44910594-44910613 Msa0072230:intron 45.0%
! GAGAGAGAGTGTGTGTGTTA+AGG - chr1_2:44911339-44911358 Msa0072230:CDS 45.0%
TGTAGATCTTCACTTGGAGG+AGG + chr1_2:44911621-44911640 None:intergenic 45.0%
! TCTCCTCGTAGCATAGCTTT+TGG + chr1_2:44910361-44910380 None:intergenic 45.0%
ATGCTACGAGGAGAAAAGAG+AGG - chr1_2:44910367-44910386 Msa0072230:intron 45.0%
GAAGCAAAAGGGTTAGGGTT+AGG + chr1_2:44910452-44910471 None:intergenic 45.0%
AGCAAAGGGTTCTACCTCAA+AGG + chr1_2:44910474-44910493 None:intergenic 45.0%
ACTGAGAAGACACAAGAGCT+TGG - chr1_2:44910593-44910612 Msa0072230:intron 45.0%
CTGAGAAGACACAAGAGCTT+GGG - chr1_2:44910594-44910613 Msa0072230:intron 45.0%
! GAGAGAGAGTGTGTGTGTTA+AGG - chr1_2:44911339-44911358 Msa0072230:CDS 45.0%
TGTAGATCTTCACTTGGAGG+AGG + chr1_2:44911621-44911640 None:intergenic 45.0%
ACGCCAAAAGCTATGCTACG+AGG - chr1_2:44910355-44910374 Msa0072230:intron 50.0%
!! TATCTCGTCGCAGAGTATCG+CGG - chr1_2:44910547-44910566 Msa0072230:intron 50.0%
GACACAAGAGCTTGGGAAGT+TGG - chr1_2:44910601-44910620 Msa0072230:intron 50.0%
GTGTTCATCTTTGGTCCACC+AGG + chr1_2:44910629-44910648 None:intergenic 50.0%
! AAGGGTCTGAAACAGCTTGC+TGG - chr1_2:44911765-44911784 Msa0072230:CDS 50.0%
! AGGGTCTGAAACAGCTTGCT+GGG - chr1_2:44911766-44911785 Msa0072230:CDS 50.0%
ACGCCAAAAGCTATGCTACG+AGG - chr1_2:44910355-44910374 Msa0072230:intron 50.0%
!! TATCTCGTCGCAGAGTATCG+CGG - chr1_2:44910547-44910566 Msa0072230:intron 50.0%
GACACAAGAGCTTGGGAAGT+TGG - chr1_2:44910601-44910620 Msa0072230:intron 50.0%
GTGTTCATCTTTGGTCCACC+AGG + chr1_2:44910629-44910648 None:intergenic 50.0%
! AAGGGTCTGAAACAGCTTGC+TGG - chr1_2:44911765-44911784 Msa0072230:CDS 50.0%
! AGGGTCTGAAACAGCTTGCT+GGG - chr1_2:44911766-44911785 Msa0072230:CDS 50.0%
!! AGAGTATCGCGGCGAGAGAA+AGG - chr1_2:44910558-44910577 Msa0072230:intron 55.0%
! GAGCTTGGGAAGTTGGTTCC+TGG - chr1_2:44910608-44910627 Msa0072230:intron 55.0%
! CTTGGGAAGTTGGTTCCTGG+TGG - chr1_2:44910611-44910630 Msa0072230:intron 55.0%
!! AGAGTATCGCGGCGAGAGAA+AGG - chr1_2:44910558-44910577 Msa0072230:intron 55.0%
! GAGCTTGGGAAGTTGGTTCC+TGG - chr1_2:44910608-44910627 Msa0072230:intron 55.0%
! CTTGGGAAGTTGGTTCCTGG+TGG - chr1_2:44910611-44910630 Msa0072230:intron 55.0%
TCGCGGCGAGAGAAAGGAGA+AGG - chr1_2:44910564-44910583 Msa0072230:intron 60.0%
TCGCGGCGAGAGAAAGGAGA+AGG - chr1_2:44910564-44910583 Msa0072230:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 44910014 44911821 44910014 ID=Msa0072230;Name=Msa0072230
chr1_2 mRNA 44910014 44911821 44910014 ID=Msa0072230-mRNA-1;Parent=Msa0072230;Name=Msa0072230-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|312
chr1_2 exon 44911099 44911821 44911099 ID=Msa0072230-mRNA-1:exon:13552;Parent=Msa0072230-mRNA-1
chr1_2 exon 44910014 44910229 44910014 ID=Msa0072230-mRNA-1:exon:13551;Parent=Msa0072230-mRNA-1
chr1_2 CDS 44911099 44911821 44911099 ID=Msa0072230-mRNA-1:cds;Parent=Msa0072230-mRNA-1
chr1_2 CDS 44910014 44910229 44910014 ID=Msa0072230-mRNA-1:cds;Parent=Msa0072230-mRNA-1
Gene Sequence

>Msa0072230

ATGGGAGCACTTAGCACATACTTCAACTATAATTGTTACCCTTATCAACCTATTCAAACTACCAACAACTTAGAAATCACTACTTTTCAACATCATGAACAAGAACAATTTGTGCAACAAAACCACCATTTTCCCTCTAACTATTTGAATGAAACTTCTCTTTTAGATGAAAGCTTCATTTTTCAAACCCCTTGTTTTTACTCAAATGAAACTTACCCTAACCCTTGTCAAGATCAACTACTTGTTGACTCCACTTTTTCTTCTCAAAATGATGGCTTTGTTTCAATGAATGAAATTTTCCCAAATGAGGAAGATTTCACCAATTACCTTACATGTCCAAAACGCCAAAAGCTATGCTACGAGGAGAAAAGAGAGGAACCACAACAAGAGCTTTTGAACTCAACCAATTTTTTTGTGGATGAGTTTATGACTAACCCTAACCCTAACCCTTTTGCTTCCTTTGAGGTAGAACCCTTTGCTGCTTCAAAAATTGTTGATGTTGTTCAATGTGAGAAGAAAGTTGGTGAAAGGACTATCTCGTCGCAGAGTATCGCGGCGAGAGAAAGGAGAAGGAAGATAACTGAGAAGACACAAGAGCTTGGGAAGTTGGTTCCTGGTGGACCAAAGATGAACACAGCAGAGATGCTTAATGCTGCTGCTAATTATGTCAAGTTTCTTCAAGCTCAAGTTGGAATGCTTCAACTTATGGAAACATTTAACAAGGAAGAAAAGGAACCTCCTCCAAGTGAAGATCTACACAAACTTCTTCTTTCTCCATTTGTTCAAGAGAAACTGTATTCAGAAGAAAAGTGCTTTGTTCCAAAAGAGTTTGTCACCACATTGAGCAATCATAATGATGTTCAATCAAAACCTACCATTCTTAAGGGTCTGAAACAGCTTGCTGGGACTGAGATTGAGAAGAAACCAAAGCAAGAATAG
Protein sequence

>Msa0072230

MGALSTYFNYNCYPYQPIQTTNNLEITTFQHHEQEQFVQQNHHFPSNYLNETSLLDESFIFQTPCFYSNETYPNPCQDQLLVDSTFSSQNDGFVSMNEIFPNEEDFTNYLTCPKRQKLCYEEKREEPQQELLNSTNFFVDEFMTNPNPNPFASFEVEPFAASKIVDVVQCEKKVGERTISSQSIAARERRRKITEKTQELGKLVPGGPKMNTAEMLNAAANYVKFLQAQVGMLQLMETFNKEEKEPPPSEDLHKLLLSPFVQEKLYSEEKCFVPKEFVTTLSNHNDVQSKPTILKGLKQLAGTEIEKKPKQE*