AlfalfaGEDB | Msa0073290

M. sativa cultivar zhongmu-4 / Msa0073290

KEGG: -
GO: -
PFAM: PF20481.1

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0073290	A0A396JYN9	55.970	268	36	7	15	279	29	217	4.77e-74	238

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TFs/TRs:

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Gene ID	Type	Classification
Msa0073290	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0073290	MtrunA17_Chr1g0180581	55.970	268	36	7	15	279	29	217	9.18e-78	238
Msa0073290	MtrunA17_Chr1g0180581	34.783	184	109	4	180	360	45	220	4.34e-13	67.8
Msa0073290	MtrunA17_Chr1g0193731	48.773	326	81	15	15	314	23	288	1.16e-60	197
Msa0073290	MtrunA17_Chr7g0214511	48.551	276	58	11	22	250	43	281	2.12e-57	188
Msa0073290	MtrunA17_Chr1g0180571	72.308	130	36	0	192	321	13	142	1.23e-54	176
Msa0073290	MtrunA17_Chr1g0169561	40.664	241	88	9	41	250	94	310	8.05e-34	127
Msa0073290	MtrunA17_Chr1g0169521	39.256	242	90	9	41	250	94	310	9.45e-30	116
Msa0073290	MtrunA17_Chr1g0174001	42.778	180	67	6	103	250	14	189	7.67e-29	110

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 58 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGAAAGAAGGGTATTT+GGG	0.313760	1_2:-46766708	None:intergenic
AAATCGAACACCCTGATCCA+AGG	0.552565	1_2:-46766935	None:intergenic
AAATTCTTCAAATCATCACT+TGG	0.555556	1_2:-46768588	None:intergenic
AAATTCTTCAAATCATTACT+TGG	0.447095	1_2:-46768705	None:intergenic
AAATTCTTCACACCATCACT+TGG	0.508172	1_2:-46768666	None:intergenic
AAATTCTTCACATCATCACT+TGG	0.564670	1_2:-46768627	None:intergenic
AAATTCTTCACATCATCACT+TGG	0.564670	1_2:-46768744	None:intergenic
AAATTCTTCACATCTTCACT+TGG	0.536473	1_2:-46768549	None:intergenic
AAATTCTTTACATCATAACT+TGG	0.493765	1_2:-46768471	None:intergenic
AACACCCTGATCCAAGGGCC+TGG	0.529902	1_2:-46766929	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGACATTCGTAAAGTATGTT+TGG	-	chr1_2:46767107-46767126	None:intergenic	30.0%
TATACCAAGAGATATGTGTT+CGG	-	chr1_2:46767185-46767204	None:intergenic	30.0%
CACCAAAGAAAATTCACTTT+CGG	+	chr1_2:46767333-46767352	Msa0073290:intron	30.0%
ACCAAAGAAAATTCACTTTC+GGG	+	chr1_2:46767334-46767353	Msa0073290:intron	30.0%
GAACAATTGTCTCATGAATT+TGG	-	chr1_2:46767385-46767404	None:intergenic	30.0%
AATTAACACGAAACTCCATA+GGG	-	chr1_2:46767493-46767512	None:intergenic	30.0%
TTTGTTTGTATGATATGTGC+TGG	-	chr1_2:46767541-46767560	None:intergenic	30.0%
TGCAATCTTTATCTGATTCT+TGG	-	chr1_2:46767585-46767604	None:intergenic	30.0%
AGTATCAAGAATGTATCGTA+GGG	-	chr1_2:46767657-46767676	None:intergenic	30.0%
AAGTATCAAGAATGTATCGT+AGG	-	chr1_2:46767658-46767677	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	46766682	46768815	46766682	ID=Msa0073290;Name=Msa0073290
chr1_2	mRNA	46766682	46768815	46766682	ID=Msa0073290-mRNA-1;Parent=Msa0073290;Name=Msa0073290-mRNA-1;_AED=0.05;_eAED=0.06;_QI=0\|0\|0\|1\|1\|1\|2\|0\|360
chr1_2	exon	46766682	46766946	46766682	ID=Msa0073290-mRNA-1:exon:14221;Parent=Msa0073290-mRNA-1
chr1_2	exon	46767998	46768815	46767998	ID=Msa0073290-mRNA-1:exon:14222;Parent=Msa0073290-mRNA-1
chr1_2	CDS	46766682	46766946	46766682	ID=Msa0073290-mRNA-1:cds;Parent=Msa0073290-mRNA-1
chr1_2	CDS	46767998	46768815	46767998	ID=Msa0073290-mRNA-1:cds;Parent=Msa0073290-mRNA-1

Gene Sequence

>Msa0073290

ATGTTCATCATAGCTCCCTTCAAAAACCCAAATACCCTTCTTTCTCTTTCATTGATTATTTACCCTAATCATAACAAAACCAAAAACATTTCTTCGACAACTAATGATGCTGGAAAACAGAAGATCATATGGCTTTTTTCTTATTCTGTTCCTAATGATGCTGGAAAACAAAAGAAACATTCATGTGATGTTTGCAACAAGGTTTTTACTTCTAACAAAGCTTTGAATGGACAATATAGTGGCGTTGCCAGGCCCTTGGATCAGGGGTTGGCAAGTGCATCCCCTCACCACAATGTGGCTCCGCCAATATGTCACTCCCCAACTGTTGCACCATCTTCCTCTGCAAGCTATGAGGATCACTTTCTACCACCTAAGAAACGGCAAGCGCTAAGAAGTAGAAGAAGTTATATGAATAATGATGATGATGCTATTGCAGCTGAAACTCTGTTGCATATATCTCGTGGCAGTTTCGAGGGATCAACTACCAATATCAATGTGAATTACCGTGGTGCTAAAAGGAAAAAACTTTTTTGCAATACGATGAATGATGATAATAATAATAAGAAGAATAAGGAACAAGATGCATTCCTAACAAGTGCTAAAGGCAAGATTTTTCAAACATCACATGATTGCAAGGAACTTGCTGCTTCTAATCATGGTATTGAAGAGAAGAAGATTAATGAAGGTGAAACAGAATTAGGGTCAAAAGTTGTGAAGAATTTTGATTTGAACGAGCTTCCAAGTTATGATGTAAAGAATTTTGATTTGAACGTGCTTCCAAGTGATGATGTGAAGAATCTTGATTTGAACAAGCTTCCAAGTGAAGATGTGAAGAATTTTGATTTAAACGAGCTTCCAAGTGATGATTTGAAGAATTTTTATTTGAACGAGCTTCCAAGTGATGATGTGAAGAATTTTGATTTGAACGAGTTTCCAAGTGATGGTGTGAAGAATTTTGATTTGAACGAGCTTCCAAGTAATGATTTGAAGAATTTTTATTTGAACGAGCTTCCAAGTGATGATGTGAAGAATTTTGATTTGAACGAGCTTCCAAATGATGATTTTGAAGATGAAACCAATTAG

Protein sequence

>Msa0073290

MFIIAPFKNPNTLLSLSLIIYPNHNKTKNISSTTNDAGKQKIIWLFSYSVPNDAGKQKKHSCDVCNKVFTSNKALNGQYSGVARPLDQGLASASPHHNVAPPICHSPTVAPSSSASYEDHFLPPKKRQALRSRRSYMNNDDDAIAAETLLHISRGSFEGSTTNINVNYRGAKRKKLFCNTMNDDNNNKKNKEQDAFLTSAKGKIFQTSHDCKELAASNHGIEEKKINEGETELGSKVVKNFDLNELPSYDVKNFDLNVLPSDDVKNLDLNKLPSEDVKNFDLNELPSDDLKNFYLNELPSDDVKNFDLNEFPSDGVKNFDLNELPSNDLKNFYLNELPSDDVKNFDLNELPNDDFEDETN*