AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0079470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0079470 MtrunA17_Chr1g0191141 85.843 1109 138 5 21 1123 8 1103 0.0 1946
Msa0079470 MtrunA17_Chr7g0224051 66.726 1127 313 16 47 1122 1 1116 0.0 1454
Msa0079470 MtrunA17_Chr3g0114161 72.430 856 208 10 292 1122 395 1247 0.0 1211
Msa0079470 MtrunA17_Chr3g0114161 64.966 294 89 4 21 300 25 318 7.81e-111 374
Msa0079470 MtrunA17_Chr5g0437621 59.836 854 308 17 297 1122 457 1303 0.0 964
Msa0079470 MtrunA17_Chr5g0437621 43.438 320 143 8 5 299 4 310 5.48e-59 222
Msa0079470 MtrunA17_Chr7g0225791 34.813 721 426 16 398 1103 577 1268 2.09e-115 388
Msa0079470 MtrunA17_Chr7g0233031 34.345 725 404 16 416 1109 549 1232 2.27e-114 384
Msa0079470 MtrunA17_Chr7g0233021 35.784 721 386 18 416 1103 537 1213 6.04e-114 383
Msa0079470 MtrunA17_Chr4g0047081 34.722 720 409 17 416 1113 548 1228 2.66e-112 379
Msa0079470 MtrunA17_Chr7g0232971 35.217 761 402 22 398 1118 584 1293 3.24e-112 379
Msa0079470 MtrunA17_Chr7g0224031 68.493 219 68 1 685 902 24 242 3.09e-96 306
Msa0079470 MtrunA17_Chr2g0296421 31.664 679 378 16 411 1078 398 1001 3.79e-91 316
Msa0079470 MtrunA17_Chr7g0232991 33.280 628 342 16 524 1109 54 646 9.29e-89 300
Msa0079470 MtrunA17_Chr8g0352361 31.143 700 387 19 394 1078 233 852 1.37e-88 305
Msa0079470 MtrunA17_Chr7g0233001 33.764 542 307 13 593 1113 3 513 1.26e-74 256
Msa0079470 MtrunA17_Chr7g0224021 70.536 112 31 1 513 624 2 111 2.17e-47 165
Msa0079470 MtrunA17_Chr2g0320781 37.247 247 139 7 831 1071 4 240 4.93e-39 145
Msa0079470 MtrunA17_Chr8g0384531 25.087 578 327 21 530 1070 372 880 8.20e-30 128
Msa0079470 MtrunA17_Chr5g0393461 24.329 633 371 24 505 1108 163 716 1.46e-29 127
Msa0079470 MtrunA17_Chr5g0403921 24.821 560 329 19 577 1121 206 688 1.04e-23 108
Msa0079470 MtrunA17_Chr1g0190391 27.731 238 144 6 536 769 576 789 4.69e-22 102
Msa0079470 MtrunA17_Chr3g0137091 25.050 499 296 16 576 1061 310 743 3.28e-20 97.4
Msa0079470 MtrunA17_Chr1g0190381 35.526 152 88 3 941 1083 205 355 9.66e-19 91.3
Msa0079470 MtrunA17_Chr5g0394201 23.346 544 329 20 577 1110 385 850 7.56e-16 83.2
Msa0079470 MtrunA17_Chr5g0442141 22.374 514 309 18 569 1067 633 1071 7.22e-15 80.1
Msa0079470 MtrunA17_Chr3g0095091 24.376 521 296 21 569 1070 587 1028 7.25e-15 80.1
Msa0079470 MtrunA17_Chr4g0066741 23.017 517 299 21 577 1080 210 640 5.07e-14 77.0
Msa0079470 MtrunA17_Chr4g0040301 31.138 167 102 4 933 1092 389 549 6.19e-14 76.6
Msa0079470 MtrunA17_Chr2g0286291 23.183 509 308 18 577 1080 213 643 1.35e-13 75.9
Msa0079470 MtrunA17_Chr3g0114111 88.235 34 4 0 1076 1109 1 34 1.84e-12 63.9
Msa0079470 MtrunA17_Chr7g0257101 29.545 176 116 5 905 1079 1173 1341 7.08e-12 70.1
Msa0079470 MtrunA17_Chr7g0255821 39.362 94 56 1 969 1061 690 783 4.06e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0079470 AT3G42670.2 57.967 797 297 12 352 1123 472 1255 0.0 900
Msa0079470 AT3G42670.2 36.333 300 162 8 4 290 2 285 2.05e-44 176
Msa0079470 AT3G42670.3 57.967 797 297 12 352 1123 472 1255 0.0 900
Msa0079470 AT3G42670.3 36.333 300 162 8 4 290 2 285 2.05e-44 176
Msa0079470 AT3G42670.4 57.967 797 299 12 352 1123 472 1257 0.0 900
Msa0079470 AT3G42670.4 36.333 300 162 8 4 290 2 285 2.22e-44 176
Msa0079470 AT3G42670.1 57.967 797 297 12 352 1123 472 1255 0.0 900
Msa0079470 AT3G42670.1 36.333 300 162 8 4 290 2 285 2.05e-44 176
Msa0079470 AT5G20420.1 55.664 821 328 10 331 1123 449 1261 0.0 886
Msa0079470 AT5G20420.1 35.443 316 156 8 4 294 2 294 1.15e-45 180
Msa0079470 AT1G05490.1 32.964 722 422 18 416 1107 695 1384 4.96e-105 361
Msa0079470 AT3G24340.1 32.222 720 421 15 405 1111 449 1114 5.35e-102 348
Msa0079470 AT2G16390.2 31.909 702 384 19 394 1078 226 850 8.70e-88 303
Msa0079470 AT2G16390.1 31.909 702 384 19 394 1078 223 847 1.04e-87 303
Msa0079470 AT2G21450.3 32.209 652 357 20 451 1078 92 682 1.35e-84 291
Msa0079470 AT2G21450.1 31.902 652 359 19 451 1078 183 773 3.09e-84 292
Msa0079470 AT2G21450.2 31.902 652 359 19 451 1078 190 780 4.88e-84 291
Msa0079470 AT3G19210.2 23.810 588 358 20 542 1102 180 704 2.57e-27 120
Msa0079470 AT3G19210.1 23.986 592 354 21 542 1102 180 706 3.45e-27 120
Msa0079470 AT3G19210.3 24.237 590 355 21 542 1102 180 706 5.71e-27 119
Msa0079470 AT5G63950.2 24.417 557 315 23 542 1070 377 855 1.02e-24 112
Msa0079470 AT5G63950.1 24.643 560 327 23 542 1070 377 872 1.07e-24 112
Msa0079470 AT1G08600.4 26.866 335 189 12 542 867 719 1006 6.77e-24 110
Msa0079470 AT1G08600.4 34.969 163 94 4 930 1083 1123 1282 1.06e-17 89.7
Msa0079470 AT1G08600.3 26.866 335 189 12 542 867 719 1006 6.77e-24 110
Msa0079470 AT1G08600.3 34.969 163 94 4 930 1083 1123 1282 1.06e-17 89.7
Msa0079470 AT1G08600.2 26.866 335 189 12 542 867 719 1006 6.77e-24 110
Msa0079470 AT1G08600.2 34.969 163 94 4 930 1083 1123 1282 1.06e-17 89.7
Msa0079470 AT5G44800.1 25.992 504 285 21 573 1061 707 1137 1.47e-21 102
Msa0079470 AT4G31900.3 23.801 542 302 19 532 1061 214 656 7.08e-20 96.7
Msa0079470 AT4G31900.1 23.801 542 302 19 532 1061 214 656 7.61e-20 96.7
Msa0079470 AT4G31900.2 23.801 542 302 19 532 1061 173 615 1.13e-19 95.9
Msa0079470 AT1G08600.1 34.969 163 94 4 930 1083 1102 1261 1.09e-17 89.7
Msa0079470 AT1G08600.1 24.024 333 180 9 542 867 719 985 6.19e-17 87.4
Msa0079470 AT2G13370.1 23.092 511 301 18 576 1070 646 1080 1.77e-15 82.4
Msa0079470 AT2G13370.2 23.092 511 301 18 576 1070 646 1080 1.77e-15 82.4
Msa0079470 AT2G13370.3 23.092 511 301 18 576 1070 646 1080 1.77e-15 82.4
Msa0079470 AT1G03750.1 30.682 176 108 5 925 1092 528 697 5.64e-15 80.5
Msa0079470 AT2G44980.1 25.000 516 301 21 583 1084 80 523 3.74e-13 74.7
Msa0079470 AT3G57300.1 30.114 176 115 5 905 1079 1187 1355 1.09e-12 73.6
Msa0079470 AT3G57300.4 30.114 176 115 5 905 1079 1187 1355 1.09e-12 73.6
Msa0079470 AT3G57300.2 30.114 176 115 5 905 1079 1220 1388 1.14e-12 73.2
Msa0079470 AT2G18760.4 31.387 137 88 3 942 1078 745 875 9.45e-12 70.1
Msa0079470 AT2G18760.1 31.387 137 88 3 942 1078 745 875 9.45e-12 70.1
Msa0079470 AT2G18760.2 31.387 137 88 3 942 1078 745 875 9.45e-12 70.1
Msa0079470 AT2G18760.7 31.387 137 88 3 942 1078 745 875 9.45e-12 70.1
Msa0079470 AT2G18760.5 31.387 137 88 3 942 1078 745 875 9.45e-12 70.1
Msa0079470 AT2G18760.6 31.387 137 88 3 942 1078 745 875 9.45e-12 70.1
Msa0079470 AT2G18760.3 31.387 137 88 3 942 1078 804 934 1.04e-11 70.1
Msa0079470 AT5G66750.1 29.940 167 112 2 942 1108 542 703 3.04e-11 68.2

Find 166 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTGTTTCAAAACAATTT+TGG 0.098097 1_2:+57377014 Msa0079470:CDS
TAAGAGAAGGTGGTTGTTTA+AGG 0.177258 1_2:+57375107 Msa0079470:CDS
CCAGGCAGAACCATTGATTT+TGG 0.203037 1_2:-57376708 None:intergenic
AGAACTTCGTGAATAAATTT+TGG 0.216798 1_2:-57377068 None:intergenic
TGCTTGACACCATTAGAATT+TGG 0.218550 1_2:-57376735 None:intergenic
AACTTTGATGTGGTACTTCT+AGG 0.221583 1_2:-57376939 None:intergenic
CTTATCTTCTGTTCTCATTA+AGG 0.232667 1_2:-57376838 None:intergenic
TATCGATTGGATGAACAAAT+TGG 0.242680 1_2:+57375850 Msa0079470:CDS
ATATGGATTTGATAGCTTTA+TGG 0.245952 1_2:+57375133 Msa0079470:CDS
AGACCTCCAATTTCGCTTCT+TGG 0.261495 1_2:-57374964 None:intergenic
TCGACCTGGTCAAGAGAAAA+TGG 0.262902 1_2:+57377892 Msa0079470:CDS
ACATCAAGTTATCTTCTTGA+TGG 0.278990 1_2:+57375435 Msa0079470:CDS
TCACTGATAGTTGGATCTTC+TGG 0.280474 1_2:-57375306 None:intergenic
AGTTATTTCGTTGAAGTCTT+TGG 0.292757 1_2:-57374630 None:intergenic
GGTAGGTGACAAGGCTAAAA+TGG 0.298106 1_2:+57376220 Msa0079470:intron
TAATGGTGTCAAGCATGATT+TGG 0.304673 1_2:+57376743 Msa0079470:CDS
TATGGATTTGATAGCTTTAT+GGG 0.316460 1_2:+57375134 Msa0079470:CDS
CCAATGCCAAGAAGCGAAAT+TGG 0.318140 1_2:+57374958 Msa0079470:CDS
CCAAAGAAAATGATAGTGTT+TGG 0.318198 1_2:+57376346 Msa0079470:CDS
TTGAAGTTCAAAACATTCAT+AGG 0.322383 1_2:-57374883 None:intergenic
CAAGCTTGTGCATCAAGTTA+TGG 0.328101 1_2:+57374837 Msa0079470:CDS
AACAAATTTGCTTTGAGAAA+AGG 0.336871 1_2:+57377521 Msa0079470:CDS
TTGGTGTCGTTGAATGTATC+TGG 0.339974 1_2:-57376689 None:intergenic
AGTTCTTCTGATACTCTTCC+TGG 0.348560 1_2:+57377287 Msa0079470:CDS
AAAGTTGAGGAAGTGCTTAA+TGG 0.350370 1_2:+57376956 Msa0079470:CDS
GCAGAAACTGCAAAAGAAAA+TGG 0.357505 1_2:+57377364 Msa0079470:CDS
GCTCTTCATTGCCACAATCT+AGG 0.360216 1_2:+57374722 Msa0079470:CDS
CCAATTTCGCTTCTTGGCAT+TGG 0.361638 1_2:-57374958 None:intergenic
AAAATTTATTCACGAAGTTC+TGG 0.379765 1_2:+57377070 Msa0079470:CDS
TTCATAGGACTACTAATATT+AGG 0.382189 1_2:-57374868 None:intergenic
GTGGTTGTTTAAGGAGCATA+TGG 0.383291 1_2:+57375116 Msa0079470:CDS
TGTCTTTCTTCACTGATAGT+TGG 0.384151 1_2:-57375315 None:intergenic
ATATGTCAATTCCAGGTCTT+AGG 0.387141 1_2:-57374264 None:intergenic
AAGCTTGTGCATCAAGTTAT+GGG 0.390339 1_2:+57374838 Msa0079470:CDS
AGGTGGTGATAATGTTTCTT+CGG 0.390951 1_2:+57375053 Msa0079470:CDS
CATGTGTAGAGTGTGGTCTT+TGG 0.395080 1_2:-57376603 None:intergenic
GCTCAAAACAAATCTTACTT+TGG 0.400942 1_2:-57377546 None:intergenic
GGTGGTGATAATGTTTCTTC+GGG 0.402375 1_2:+57375054 Msa0079470:CDS
AATTCCAGGTCTTAGGAAAC+TGG 0.403179 1_2:-57374257 None:intergenic
CCTGGAGTTCCAAATTCTAA+TGG 0.403216 1_2:+57376726 Msa0079470:CDS
AAGAAAATGGCGGGCTGTAC+CGG 0.405933 1_2:+57377377 Msa0079470:CDS
CAAAGAAAATGATAGTGTTT+GGG 0.407919 1_2:+57376347 Msa0079470:CDS
GGTAGAAAATCCTGATGATT+TGG 0.409165 1_2:+57375380 Msa0079470:CDS
GTCTTTGGAGCTAGGACTAA+TGG 0.409580 1_2:-57376588 None:intergenic
AAAGAGATTCTGCAACTAAC+TGG 0.420585 1_2:+57377680 Msa0079470:CDS
CTCAACCTCAAGAGAGTAAC+CGG 0.423138 1_2:-57377396 None:intergenic
ATCATGTTCACATGTTTCCT+TGG 0.426716 1_2:-57375827 None:intergenic
CACCCTAGTGTTCTTGTCAT+GGG 0.427375 1_2:+57376798 Msa0079470:CDS
TCAACTGATGCTACTGATCT+TGG 0.430086 1_2:+57376312 Msa0079470:CDS
ACGCGCGAGAAAATTCTTCT+TGG 0.431056 1_2:+57377157 Msa0079470:CDS
AAGGAAGAGGATAATGTGAT+TGG 0.437162 1_2:+57374586 Msa0079470:CDS
TACGTAATGTTCAAGTTGTA+TGG 0.439233 1_2:-57375007 None:intergenic
AAGAAAAGAGATGATGAGAA+AGG 0.450749 1_2:-57376532 None:intergenic
ACACCCTAGTGTTCTTGTCA+TGG 0.450907 1_2:+57376797 Msa0079470:CDS
TCTATATTTATCTTCCTCCA+TGG 0.451446 1_2:-57377945 None:intergenic
TTGAACTTCAAATCAGAAAA+CGG 0.451497 1_2:+57374895 Msa0079470:CDS
CTGATGATTTGGCTGGCATA+TGG 0.454793 1_2:+57375391 Msa0079470:CDS
AGCACAGATATGTCAATTCC+AGG 0.458031 1_2:-57374271 None:intergenic
ATATCTCATGCTCCTGGAGC+TGG 0.462480 1_2:+57376504 Msa0079470:CDS
TACTATGAGTTTGTTGTGCA+TGG 0.462547 1_2:-57374180 None:intergenic
TCAATCAATGCTTGTTCTGA+AGG 0.465693 1_2:+57377782 Msa0079470:CDS
TATCGATGATCATGAAGAAC+AGG 0.466478 1_2:+57375203 Msa0079470:CDS
GCATTTGAAGCAGTATTGAA+TGG 0.467351 1_2:+57374097 Msa0079470:intron
AGTAAAATTCTCAATGCTGC+TGG 0.475556 1_2:-57376984 None:intergenic
GTATATCACTTGTTGATGAC+TGG 0.488667 1_2:+57377920 Msa0079470:CDS
AGAGTGTGGTCTTTGGAGCT+AGG 0.489103 1_2:-57376596 None:intergenic
CACAATCTCGTGTTGTTCAT+CGG 0.491623 1_2:-57377342 None:intergenic
CCATTAGAATTTGGAACTCC+AGG 0.495262 1_2:-57376726 None:intergenic
TACCGCGTCATCTTTAAAGA+AGG 0.495527 1_2:+57374786 Msa0079470:CDS
CATATGCCAGCCAAATCATC+AGG 0.497067 1_2:-57375390 None:intergenic
ACGTGTGATGAGCAAGGAAA+AGG 0.497284 1_2:+57377577 Msa0079470:CDS
AATCTTCTCAGCTTGCCATT+CGG 0.505551 1_2:-57378032 None:intergenic
TATTTCGTTGAAGTCTTTGG+AGG 0.508106 1_2:-57374627 None:intergenic
AGATTGTAATGATGAAAAGT+TGG 0.511319 1_2:+57375029 Msa0079470:CDS
GTAATTTGTCATCAGACATC+CGG 0.513031 1_2:+57374200 Msa0079470:CDS
GCGCATGGCTAAAGCATTGA+GGG 0.513248 1_2:+57376878 Msa0079470:CDS
TGCTCCAGTTTCCTAAGACC+TGG 0.515668 1_2:+57374253 Msa0079470:CDS
CATGAAATCGACATGAACAT+TGG 0.516088 1_2:-57374513 None:intergenic
CTTGTTGATGACTGGATCCA+TGG 0.517661 1_2:+57377928 Msa0079470:CDS
GAAGAACTACTTGGAAAGAG+TGG 0.517977 1_2:+57377966 Msa0079470:CDS
AAGCAGTATTGAATGGCTCA+TGG 0.520661 1_2:+57374104 Msa0079470:CDS
GAAACAATGCAGTGAAGATA+CGG 0.522442 1_2:+57376254 Msa0079470:CDS
CCAAAATCAATGGTTCTGCC+TGG 0.525654 1_2:+57376708 Msa0079470:CDS
TGTGTTATATCTCATGCTCC+TGG 0.525908 1_2:+57376498 Msa0079470:CDS
TTATCTTCTTGATGGAAATG+AGG 0.528274 1_2:+57375443 Msa0079470:CDS
TGGATAAGATACACCGGCAC+GGG 0.534854 1_2:-57376654 None:intergenic
GGTTGCATTTGTCATAAGAT+CGG 0.534985 1_2:+57374807 Msa0079470:CDS
TGAAGAAAGACAGTTTGAGC+AGG 0.538289 1_2:+57375326 Msa0079470:CDS
TATGATGATCAAGGTTCACT+TGG 0.538490 1_2:+57374544 Msa0079470:CDS
TTCAACGACACCAAAATCAA+TGG 0.539455 1_2:+57376698 Msa0079470:CDS
CTGTACCGGTTACTCTCTTG+AGG 0.542553 1_2:+57377391 Msa0079470:CDS
TGATGATTTGGCTGGCATAT+GGG 0.542896 1_2:+57375392 Msa0079470:CDS
TTCTCAGCTTGCCATTCGGA+AGG 0.544538 1_2:-57378028 None:intergenic
TTGTGCATGGAGGAAATGTA+TGG 0.544715 1_2:-57374167 None:intergenic
CGTATCGAAAAGCGGTGATG+AGG 0.549235 1_2:+57375359 Msa0079470:CDS
GAAAATCCTGATGATTTGGC+TGG 0.557990 1_2:+57375384 Msa0079470:CDS
GTCTAGAAAAGATCAAAAGC+TGG 0.560297 1_2:+57376769 Msa0079470:CDS
TTACGATAATTAGCATCGAA+TGG 0.560548 1_2:-57373606 None:intergenic
ATAAATATAGAAGAACTACT+TGG 0.563098 1_2:+57377957 Msa0079470:CDS
TTAGATTCTAAGTACAGAAG+AGG 0.564016 1_2:+57377098 Msa0079470:CDS
AGATTGAAGATGAAATACTC+AGG 0.564407 1_2:+57378050 Msa0079470:CDS
TGACACAAATAAAGAATCCA+AGG 0.564564 1_2:+57375810 Msa0079470:CDS
AATGTATAGATTTGCAAACC+AGG 0.566512 1_2:-57377305 None:intergenic
AAGAACTACTTGGAAAGAGT+GGG 0.567137 1_2:+57377967 Msa0079470:CDS
GCGCGTGCCTCTAGTAAATG+AGG 0.568077 1_2:-57377140 None:intergenic
TCATCAAGTATCAAGAGTCC+AGG 0.569191 1_2:-57376906 None:intergenic
TAACCCATGACAAGAACACT+AGG 0.569323 1_2:-57376801 None:intergenic
AGCGCATGGCTAAAGCATTG+AGG 0.574680 1_2:+57376877 Msa0079470:CDS
TGCTCATCACACGTGAGATT+AGG 0.575173 1_2:-57377568 None:intergenic
GGAAGTAATCGATGCTTACA+TGG 0.577289 1_2:+57375266 Msa0079470:CDS
GAACATTGGTTATATTTGTG+AGG 0.579746 1_2:-57374499 None:intergenic
AAACTGCAAAAGAAAATGGC+GGG 0.587027 1_2:+57377368 Msa0079470:CDS
GTATCTGGCTTCGACAGCTG+TGG 0.588022 1_2:-57376674 None:intergenic
ATATCCAATGGTAGGTGACA+AGG 0.588447 1_2:+57376211 Msa0079470:intron
AGCAGTATTGAATGGCTCAT+GGG 0.590169 1_2:+57374105 Msa0079470:CDS
AAAGCATTGAGGGAAAGTCC+TGG 0.590693 1_2:+57376888 Msa0079470:CDS
GCACTTCCTCAACTTTGATG+TGG 0.590805 1_2:-57376949 None:intergenic
CCGAAAAGTAACCTAGATTG+TGG 0.592635 1_2:-57374733 None:intergenic
AGATGAAATACTCAGGGAGA+TGG 0.595348 1_2:+57378057 Msa0079470:CDS
CTTCAACGAAATAACTGTGA+AGG 0.595665 1_2:+57374637 Msa0079470:CDS
CAAAGAAGCTTAAGAGAAGG+TGG 0.596830 1_2:+57375097 Msa0079470:CDS
ATGCCAAGAAGCGAAATTGG+AGG 0.597152 1_2:+57374961 Msa0079470:CDS
AAAATGGAATATTCCCGTGC+CGG 0.598414 1_2:+57376641 Msa0079470:CDS
AGCATCGATTACTTCCTCGC+AGG 0.600178 1_2:-57375259 None:intergenic
GATAAATTTGAAGATCGTTG+TGG 0.605781 1_2:+57377737 Msa0079470:CDS
TGATAGTTGGATCTTCTGGT+AGG 0.608458 1_2:-57375302 None:intergenic
TGGACTCTTGATACTTGATG+AGG 0.608858 1_2:+57376908 Msa0079470:CDS
GGAATATACTGCCGTACATG+CGG 0.610029 1_2:+57375871 Msa0079470:CDS
ACTCAGGGAGATGGTTGAAG+AGG 0.610175 1_2:+57378066 Msa0079470:CDS
AGATGACGCGGTAACAAACC+AGG 0.611437 1_2:-57374776 None:intergenic
GCAGGGTGCAAATGCTGATG+AGG 0.611917 1_2:+57375780 Msa0079470:intron
CAAAGACCACACTCTACACA+TGG 0.613075 1_2:+57376604 Msa0079470:CDS
CACTCGGGTCAATACACCCA+TGG 0.615844 1_2:+57377429 Msa0079470:CDS
CAGAAGAGGCAAACTAGAGG+AGG 0.618526 1_2:+57377112 Msa0079470:CDS
AAATTCACACAGAAAGCGCA+TGG 0.619203 1_2:+57376863 Msa0079470:CDS
GAGCATGATTATGACGGAGA+AGG 0.621402 1_2:+57374925 Msa0079470:CDS
AATCTCACGTGTGATGAGCA+AGG 0.622148 1_2:+57377571 Msa0079470:CDS
TCACTTGAGCATGATTATGA+CGG 0.623465 1_2:+57374919 Msa0079470:CDS
GATTGAAGATGAAATACTCA+GGG 0.623647 1_2:+57378051 Msa0079470:CDS
CATGAAGAACAGGATCATAG+AGG 0.626014 1_2:+57375213 Msa0079470:CDS
GAAGCGATACAATCCAAAGA+AGG 0.629502 1_2:+57375156 Msa0079470:CDS
AATGAACTCAAATATTGATG+CGG 0.633014 1_2:+57377196 Msa0079470:CDS
ATGTGAACATGATTATCGAT+TGG 0.633134 1_2:+57375837 Msa0079470:CDS
GAAACTGCAAAAGAAAATGG+CGG 0.634136 1_2:+57377367 Msa0079470:CDS
GTACAGAAGAGGCAAACTAG+AGG 0.636952 1_2:+57377109 Msa0079470:CDS
GGACTCTTGATACTTGATGA+GGG 0.637751 1_2:+57376909 Msa0079470:CDS
GCACAAAGAAGCTTAAGAGA+AGG 0.639100 1_2:+57375094 Msa0079470:CDS
GAATGGCTCATGGGAACCTG+TGG 0.639123 1_2:+57374114 Msa0079470:CDS
AAAGAGAAATCTGACACAAG+TGG 0.641751 1_2:+57376471 Msa0079470:CDS
GTCACAACCTTGCACCAGTG+AGG 0.646068 1_2:+57377612 Msa0079470:CDS
TGTAATGATGAAAAGTTGGA+AGG 0.659731 1_2:+57375033 Msa0079470:CDS
GAAGTACCACATCAAAGTTG+AGG 0.659873 1_2:+57376943 Msa0079470:CDS
GTGGATAAGATACACCGGCA+CGG 0.666067 1_2:-57376655 None:intergenic
TTGTTGAAGATCCTTCCGAA+TGG 0.668864 1_2:+57378017 Msa0079470:CDS
AACCTTCTTTAAAGATGACG+CGG 0.674091 1_2:-57374788 None:intergenic
AAAGAAAACGTATCGAAAAG+CGG 0.683079 1_2:+57375351 Msa0079470:CDS
GTCTACGACGCTCAAAACGT+AGG 0.683290 1_2:+57374983 Msa0079470:CDS
CAGCTGTGGATAAGATACAC+CGG 0.684401 1_2:-57376660 None:intergenic
GAGAAATCTGACACAAGTGG+TGG 0.685073 1_2:+57376474 Msa0079470:CDS
AACCCATGACAAGAACACTA+GGG 0.686530 1_2:-57376800 None:intergenic
AATGATGAAAAGTTGGAAGG+TGG 0.696462 1_2:+57375036 Msa0079470:CDS
GGAAGCGCCTCATTTACTAG+AGG 0.697989 1_2:+57377133 Msa0079470:CDS
TATGAGTTTGTTGTGCATGG+AGG 0.703519 1_2:-57374177 None:intergenic
ATGACGGAGAAGGTGCAATG+CGG 0.752637 1_2:+57374935 Msa0079470:CDS
GTTAAATGCTGATACCTGCG+AGG 0.758695 1_2:+57375245 Msa0079470:CDS
GTTGATGACTGGATCCATGG+AGG 0.768053 1_2:+57377931 Msa0079470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATGCAAATAATTTTTTT+CGG - chr1_2:57373577-57373596 None:intergenic 15.0%
!! AATAAATAAATGTATACAGA+AGG - chr1_2:57374399-57374418 None:intergenic 15.0%
!!! GTAAAACATTTTATACATAT+AGG - chr1_2:57375549-57375568 None:intergenic 15.0%
!! ATATGACAATTATGATTTAT+AGG + chr1_2:57375644-57375663 Msa0079470:intron 15.0%
!!! GATTTAAATACGTTATTTTT+TGG + chr1_2:57375701-57375720 Msa0079470:intron 15.0%
!!! TGATGCAAATAATTTTTTTC+GGG - chr1_2:57373576-57373595 None:intergenic 20.0%
!! AGTTTATGTTAATGAATTAC+CGG - chr1_2:57373870-57373889 None:intergenic 20.0%
!! TGTTTAGAAATAAGAATTAG+AGG - chr1_2:57374077-57374096 None:intergenic 20.0%
!!! AACTCATAAAAATTGATTCT+GGG + chr1_2:57374137-57374156 Msa0079470:CDS 20.0%
!!! TGAAGTTAATTCTTTTAGAA+GGG + chr1_2:57374684-57374703 Msa0079470:CDS 20.0%
!!! TTTTTTACTTGACTTACAAT+GGG - chr1_2:57375931-57375950 None:intergenic 20.0%
!!! TAATTACAAGTTTGAAATCA+CGG - chr1_2:57376087-57376106 None:intergenic 20.0%
!! TGTAAATTAAGTTCCTTATT+TGG + chr1_2:57376111-57376130 Msa0079470:intron 20.0%
!!! TTGTTACATATTTTCTCTAT+TGG + chr1_2:57376177-57376196 Msa0079470:intron 20.0%
!!! ACTTTGTTTCAAAACAATTT+TGG + chr1_2:57377014-57377033 Msa0079470:CDS 20.0%
!!! CTTTGTTTCAAAACAATTTT+GGG + chr1_2:57377015-57377034 Msa0079470:CDS 20.0%
!!! TTTGTTTCAAAACAATTTTG+GGG + chr1_2:57377016-57377035 Msa0079470:CDS 20.0%
!! ATAAATATAGAAGAACTACT+TGG + chr1_2:57377957-57377976 Msa0079470:CDS 20.0%
!! GTATGATATATTTAAGATGC+CGG + chr1_2:57373848-57373867 Msa0079470:intron 25.0%
! AATGTAATTGACAATCTTGT+AGG + chr1_2:57373978-57373997 Msa0079470:intron 25.0%
!! GAACTCATAAAAATTGATTC+TGG + chr1_2:57374136-57374155 Msa0079470:CDS 25.0%
!! GTAAACTTTTATGATGATCA+AGG + chr1_2:57374535-57374554 Msa0079470:CDS 25.0%
!! CTGAAGTTAATTCTTTTAGA+AGG + chr1_2:57374683-57374702 Msa0079470:CDS 25.0%
! TTCATAGGACTACTAATATT+AGG - chr1_2:57374871-57374890 None:intergenic 25.0%
! TTGAAGTTCAAAACATTCAT+AGG - chr1_2:57374886-57374905 None:intergenic 25.0%
! TTGAACTTCAAATCAGAAAA+CGG + chr1_2:57374895-57374914 Msa0079470:CDS 25.0%
! AGATTGTAATGATGAAAAGT+TGG + chr1_2:57375029-57375048 Msa0079470:CDS 25.0%
!! ATATGGATTTGATAGCTTTA+TGG + chr1_2:57375133-57375152 Msa0079470:CDS 25.0%
!!! TATGGATTTGATAGCTTTAT+GGG + chr1_2:57375134-57375153 Msa0079470:CDS 25.0%
!! TATTTGACTTCAACCATAAA+TGG - chr1_2:57375615-57375634 None:intergenic 25.0%
! TGACAATTATGATTTATAGG+CGG + chr1_2:57375647-57375666 Msa0079470:intron 25.0%
!!! TGGTTTTATCGTCTATTTTA+AGG + chr1_2:57375721-57375740 Msa0079470:intron 25.0%
! AACTATTAAATACCATAGCA+GGG + chr1_2:57375763-57375782 Msa0079470:intron 25.0%
!!! GTTTTTTACTTGACTTACAA+TGG - chr1_2:57375932-57375951 None:intergenic 25.0%
!! AATTAAGTTCCTTATTTGGT+TGG + chr1_2:57376115-57376134 Msa0079470:intron 25.0%
! AAAACATATCCAACCAAATA+AGG - chr1_2:57376127-57376146 None:intergenic 25.0%
! GTTGTCTAATTTATATCCAA+TGG + chr1_2:57376199-57376218 Msa0079470:intron 25.0%
! TCTAATTTATATCCAATGGT+AGG + chr1_2:57376203-57376222 Msa0079470:intron 25.0%
!! CTATCATTTTCTTTGGATAT+AGG - chr1_2:57376342-57376361 None:intergenic 25.0%
!! CAAAGAAAATGATAGTGTTT+GGG + chr1_2:57376347-57376366 Msa0079470:CDS 25.0%
!! AGAACTTCGTGAATAAATTT+TGG - chr1_2:57377071-57377090 None:intergenic 25.0%
! AAAATTTATTCACGAAGTTC+TGG + chr1_2:57377070-57377089 Msa0079470:CDS 25.0%
! AATGAACTCAAATATTGATG+CGG + chr1_2:57377196-57377215 Msa0079470:CDS 25.0%
!!! AACAAATTTGCTTTGAGAAA+AGG + chr1_2:57377521-57377540 Msa0079470:CDS 25.0%
!! TATTTGAGAATCATTTTCAG+TGG + chr1_2:57377648-57377667 Msa0079470:CDS 25.0%
!! AATCATTTTCAGTGGAAAAA+TGG + chr1_2:57377656-57377675 Msa0079470:CDS 25.0%
! TTACGATAATTAGCATCGAA+TGG - chr1_2:57373609-57373628 None:intergenic 30.0%
ATACGATAATAGCGCTATAA+TGG + chr1_2:57373765-57373784 Msa0079470:intron 30.0%
! TCAATTTTTATGAGTTCCAC+AGG - chr1_2:57374133-57374152 None:intergenic 30.0%
! TAGCTTTTCTTGATCTTATC+CGG - chr1_2:57374222-57374241 None:intergenic 30.0%
GTAAGAAGATTGAACTTACT+GGG - chr1_2:57374336-57374355 None:intergenic 30.0%
AGTAAGAAGATTGAACTTAC+TGG - chr1_2:57374337-57374356 None:intergenic 30.0%
!!! TTTGTGAGGTTTTTTGTGTA+TGG - chr1_2:57374488-57374507 None:intergenic 30.0%
! GAACATTGGTTATATTTGTG+AGG - chr1_2:57374502-57374521 None:intergenic 30.0%
CACTGAAATAAAAACTCTGA+AGG + chr1_2:57374567-57374586 Msa0079470:CDS 30.0%
! AATAAAAACTCTGAAGGAAG+AGG + chr1_2:57374573-57374592 Msa0079470:CDS 30.0%
! AGTTATTTCGTTGAAGTCTT+TGG - chr1_2:57374633-57374652 None:intergenic 30.0%
!! TACGTAATGTTCAAGTTGTA+TGG - chr1_2:57375010-57375029 None:intergenic 30.0%
TGTAATGATGAAAAGTTGGA+AGG + chr1_2:57375033-57375052 Msa0079470:CDS 30.0%
! TTTCTTTTGAACACCTTCTT+TGG - chr1_2:57375172-57375191 None:intergenic 30.0%
AAAGAAAACGTATCGAAAAG+CGG + chr1_2:57375351-57375370 Msa0079470:CDS 30.0%
ACATCAAGTTATCTTCTTGA+TGG + chr1_2:57375435-57375454 Msa0079470:CDS 30.0%
TTATCTTCTTGATGGAAATG+AGG + chr1_2:57375443-57375462 Msa0079470:CDS 30.0%
ATTGACTGTTGAACCATTTA+TGG + chr1_2:57375599-57375618 Msa0079470:intron 30.0%
!!! ATCGTCTATTTTAAGGCTAA+AGG + chr1_2:57375728-57375747 Msa0079470:intron 30.0%
CAACTATTAAATACCATAGC+AGG + chr1_2:57375762-57375781 Msa0079470:intron 30.0%
TGACACAAATAAAGAATCCA+AGG + chr1_2:57375810-57375829 Msa0079470:CDS 30.0%
ATGTGAACATGATTATCGAT+TGG + chr1_2:57375837-57375856 Msa0079470:CDS 30.0%
! TATCGATTGGATGAACAAAT+TGG + chr1_2:57375850-57375869 Msa0079470:CDS 30.0%
!!! GTTTTAATTAGCAACGAGTT+TGG + chr1_2:57375971-57375990 Msa0079470:intron 30.0%
TAACTTTCAGAAAATCACAG+TGG - chr1_2:57376012-57376031 None:intergenic 30.0%
! CCAAACACTATCATTTTCTT+TGG - chr1_2:57376349-57376368 None:intergenic 30.0%
! CCAAAGAAAATGATAGTGTT+TGG + chr1_2:57376346-57376365 Msa0079470:CDS 30.0%
!! AAAAAGCTTTCGAGTTTCTT+TGG + chr1_2:57376412-57376431 Msa0079470:CDS 30.0%
! TTTCTTTGGAAAAACATTGC+AGG + chr1_2:57376426-57376445 Msa0079470:CDS 30.0%
! TTCTTTGGAAAAACATTGCA+GGG + chr1_2:57376427-57376446 Msa0079470:CDS 30.0%
AAGAAAAGAGATGATGAGAA+AGG - chr1_2:57376535-57376554 None:intergenic 30.0%
CTTATCTTCTGTTCTCATTA+AGG - chr1_2:57376841-57376860 None:intergenic 30.0%
! GCATTGAGAATTTTACTTTC+TGG + chr1_2:57376990-57377009 Msa0079470:CDS 30.0%
!! TTCGTGAATAAATTTTGGTC+TGG - chr1_2:57377066-57377085 None:intergenic 30.0%
TTAGATTCTAAGTACAGAAG+AGG + chr1_2:57377098-57377117 Msa0079470:CDS 30.0%
TATTGATGCGGAAAAAATGA+AGG + chr1_2:57377208-57377227 Msa0079470:CDS 30.0%
ATTGATGCGGAAAAAATGAA+GGG + chr1_2:57377209-57377228 Msa0079470:CDS 30.0%
AATGTATAGATTTGCAAACC+AGG - chr1_2:57377308-57377327 None:intergenic 30.0%
!!! TAGTTTTTTCAACTCTTCAC+CGG - chr1_2:57377495-57377514 None:intergenic 30.0%
GCTCAAAACAAATCTTACTT+TGG - chr1_2:57377549-57377568 None:intergenic 30.0%
AACTCTATTAAAAACCTCAC+TGG - chr1_2:57377629-57377648 None:intergenic 30.0%
GATAAATTTGAAGATCGTTG+TGG + chr1_2:57377737-57377756 Msa0079470:CDS 30.0%
!!! ATAGCTTGCTTTGTTTTTGA+AGG - chr1_2:57377860-57377879 None:intergenic 30.0%
TCTATATTTATCTTCCTCCA+TGG - chr1_2:57377948-57377967 None:intergenic 30.0%
! TTCTTGTATGATTTTCAGTG+AGG + chr1_2:57377991-57378010 Msa0079470:CDS 30.0%
AGATTGAAGATGAAATACTC+AGG + chr1_2:57378050-57378069 Msa0079470:CDS 30.0%
GATTGAAGATGAAATACTCA+GGG + chr1_2:57378051-57378070 Msa0079470:CDS 30.0%
!! AGCGCTATAATGGATATTCA+TGG + chr1_2:57373775-57373794 Msa0079470:intron 35.0%
TAACTAGATGTGCTATGCAT+TGG + chr1_2:57373928-57373947 Msa0079470:intron 35.0%
! GCATTTGAAGCAGTATTGAA+TGG + chr1_2:57374097-57374116 Msa0079470:intron 35.0%
!! TACTATGAGTTTGTTGTGCA+TGG - chr1_2:57374183-57374202 None:intergenic 35.0%
! GTAATTTGTCATCAGACATC+CGG + chr1_2:57374200-57374219 Msa0079470:CDS 35.0%
ATATGTCAATTCCAGGTCTT+AGG - chr1_2:57374267-57374286 None:intergenic 35.0%
CATGAAATCGACATGAACAT+TGG - chr1_2:57374516-57374535 None:intergenic 35.0%
TATGATGATCAAGGTTCACT+TGG + chr1_2:57374544-57374563 Msa0079470:CDS 35.0%
AAGGAAGAGGATAATGTGAT+TGG + chr1_2:57374586-57374605 Msa0079470:CDS 35.0%
TATTTCGTTGAAGTCTTTGG+AGG - chr1_2:57374630-57374649 None:intergenic 35.0%
CTTCAACGAAATAACTGTGA+AGG + chr1_2:57374637-57374656 Msa0079470:CDS 35.0%
!!! CCACAATCTAGGTTACTTTT+CGG + chr1_2:57374733-57374752 Msa0079470:CDS 35.0%
!!! CACAATCTAGGTTACTTTTC+GGG + chr1_2:57374734-57374753 Msa0079470:CDS 35.0%
! AGTTTTTGTCTGATCTTTCC+TGG + chr1_2:57374758-57374777 Msa0079470:CDS 35.0%
! AACCTTCTTTAAAGATGACG+CGG - chr1_2:57374791-57374810 None:intergenic 35.0%
GGTTGCATTTGTCATAAGAT+CGG + chr1_2:57374807-57374826 Msa0079470:CDS 35.0%
AAGCTTGTGCATCAAGTTAT+GGG + chr1_2:57374838-57374857 Msa0079470:CDS 35.0%
TCACTTGAGCATGATTATGA+CGG + chr1_2:57374919-57374938 Msa0079470:CDS 35.0%
AATGATGAAAAGTTGGAAGG+TGG + chr1_2:57375036-57375055 Msa0079470:CDS 35.0%
! AGGTGGTGATAATGTTTCTT+CGG + chr1_2:57375053-57375072 Msa0079470:CDS 35.0%
TAAGAGAAGGTGGTTGTTTA+AGG + chr1_2:57375107-57375126 Msa0079470:CDS 35.0%
TATCGATGATCATGAAGAAC+AGG + chr1_2:57375203-57375222 Msa0079470:CDS 35.0%
TGTCTTTCTTCACTGATAGT+TGG - chr1_2:57375318-57375337 None:intergenic 35.0%
GGTAGAAAATCCTGATGATT+TGG + chr1_2:57375380-57375399 Msa0079470:CDS 35.0%
TGACAAGTAAAAGACGAGAT+GGG - chr1_2:57375490-57375509 None:intergenic 35.0%
ATGACAAGTAAAAGACGAGA+TGG - chr1_2:57375491-57375510 None:intergenic 35.0%
ATCATGTTCACATGTTTCCT+TGG - chr1_2:57375830-57375849 None:intergenic 35.0%
! TTACAAGTTTGAAATCACGG+TGG - chr1_2:57376084-57376103 None:intergenic 35.0%
GAAACAATGCAGTGAAGATA+CGG + chr1_2:57376254-57376273 Msa0079470:CDS 35.0%
!! TGCATGTTTTCTTTGAGTTC+AGG - chr1_2:57376381-57376400 None:intergenic 35.0%
!!! GAAACTCGAAAGCTTTTTTC+TGG - chr1_2:57376410-57376429 None:intergenic 35.0%
AAAGAGAAATCTGACACAAG+TGG + chr1_2:57376471-57376490 Msa0079470:CDS 35.0%
GGCAAAAAGAGTTCGAAAAA+TGG + chr1_2:57376625-57376644 Msa0079470:CDS 35.0%
TTCAACGACACCAAAATCAA+TGG + chr1_2:57376698-57376717 Msa0079470:CDS 35.0%
TGCTTGACACCATTAGAATT+TGG - chr1_2:57376738-57376757 None:intergenic 35.0%
! TAATGGTGTCAAGCATGATT+TGG + chr1_2:57376743-57376762 Msa0079470:CDS 35.0%
GTCTAGAAAAGATCAAAAGC+TGG + chr1_2:57376769-57376788 Msa0079470:CDS 35.0%
AACTTTGATGTGGTACTTCT+AGG - chr1_2:57376942-57376961 None:intergenic 35.0%
!! AAAGTTGAGGAAGTGCTTAA+TGG + chr1_2:57376956-57376975 Msa0079470:CDS 35.0%
AGTAAAATTCTCAATGCTGC+TGG - chr1_2:57376987-57377006 None:intergenic 35.0%
GTGCTTCGAAAAATCACAAA+TGG + chr1_2:57377239-57377258 Msa0079470:CDS 35.0%
GCAGAAACTGCAAAAGAAAA+TGG + chr1_2:57377364-57377383 Msa0079470:CDS 35.0%
GAAACTGCAAAAGAAAATGG+CGG + chr1_2:57377367-57377386 Msa0079470:CDS 35.0%
AAACTGCAAAAGAAAATGGC+GGG + chr1_2:57377368-57377387 Msa0079470:CDS 35.0%
!! GTTGAGCTTTTGATAACACT+CGG + chr1_2:57377413-57377432 Msa0079470:CDS 35.0%
!! TTGAGCTTTTGATAACACTC+GGG + chr1_2:57377414-57377433 Msa0079470:CDS 35.0%
AAAGAGATTCTGCAACTAAC+TGG + chr1_2:57377680-57377699 Msa0079470:CDS 35.0%
TCAATCAATGCTTGTTCTGA+AGG + chr1_2:57377782-57377801 Msa0079470:CDS 35.0%
! AGCTTCAAGAGTGATTTTCT+TGG + chr1_2:57377820-57377839 Msa0079470:CDS 35.0%
! TGATTTTCTTGGATTCTGAG+TGG + chr1_2:57377831-57377850 Msa0079470:CDS 35.0%
! TAAACCATTTTCTCTTGACC+AGG - chr1_2:57377899-57377918 None:intergenic 35.0%
!! GTATATCACTTGTTGATGAC+TGG + chr1_2:57377920-57377939 Msa0079470:CDS 35.0%
AAGAACTACTTGGAAAGAGT+GGG + chr1_2:57377967-57377986 Msa0079470:CDS 35.0%
GATACATGTGTCTAACTCGT+TGG + chr1_2:57373810-57373829 Msa0079470:intron 40.0%
! AAGCAGTATTGAATGGCTCA+TGG + chr1_2:57374104-57374123 Msa0079470:CDS 40.0%
! AGCAGTATTGAATGGCTCAT+GGG + chr1_2:57374105-57374124 Msa0079470:CDS 40.0%
TTGTGCATGGAGGAAATGTA+TGG - chr1_2:57374170-57374189 None:intergenic 40.0%
!! TATGAGTTTGTTGTGCATGG+AGG - chr1_2:57374180-57374199 None:intergenic 40.0%
AATTCCAGGTCTTAGGAAAC+TGG - chr1_2:57374260-57374279 None:intergenic 40.0%
AGCACAGATATGTCAATTCC+AGG - chr1_2:57374274-57374293 None:intergenic 40.0%
CCGAAAAGTAACCTAGATTG+TGG - chr1_2:57374736-57374755 None:intergenic 40.0%
! TACCGCGTCATCTTTAAAGA+AGG + chr1_2:57374786-57374805 Msa0079470:CDS 40.0%
CAAGCTTGTGCATCAAGTTA+TGG + chr1_2:57374837-57374856 Msa0079470:CDS 40.0%
! GGTGGTGATAATGTTTCTTC+GGG + chr1_2:57375054-57375073 Msa0079470:CDS 40.0%
GCACAAAGAAGCTTAAGAGA+AGG + chr1_2:57375094-57375113 Msa0079470:CDS 40.0%
CAAAGAAGCTTAAGAGAAGG+TGG + chr1_2:57375097-57375116 Msa0079470:CDS 40.0%
! GTGGTTGTTTAAGGAGCATA+TGG + chr1_2:57375116-57375135 Msa0079470:CDS 40.0%
GAAGCGATACAATCCAAAGA+AGG + chr1_2:57375156-57375175 Msa0079470:CDS 40.0%
CATGAAGAACAGGATCATAG+AGG + chr1_2:57375213-57375232 Msa0079470:CDS 40.0%
GGAAGTAATCGATGCTTACA+TGG + chr1_2:57375266-57375285 Msa0079470:CDS 40.0%
TGATAGTTGGATCTTCTGGT+AGG - chr1_2:57375305-57375324 None:intergenic 40.0%
TCACTGATAGTTGGATCTTC+TGG - chr1_2:57375309-57375328 None:intergenic 40.0%
TGAAGAAAGACAGTTTGAGC+AGG + chr1_2:57375326-57375345 Msa0079470:CDS 40.0%
! GAAAATCCTGATGATTTGGC+TGG + chr1_2:57375384-57375403 Msa0079470:CDS 40.0%
! TGATGATTTGGCTGGCATAT+GGG + chr1_2:57375392-57375411 Msa0079470:CDS 40.0%
! TTTTCACAAAGCCGCATGTA+CGG - chr1_2:57375885-57375904 None:intergenic 40.0%
ATATCCAATGGTAGGTGACA+AGG + chr1_2:57376211-57376230 Msa0079470:intron 40.0%
TCAACTGATGCTACTGATCT+TGG + chr1_2:57376312-57376331 Msa0079470:CDS 40.0%
GAAAAACATTGCAGGGTCTA+TGG + chr1_2:57376434-57376453 Msa0079470:CDS 40.0%
TGTGTTATATCTCATGCTCC+TGG + chr1_2:57376498-57376517 Msa0079470:CDS 40.0%
! AGGACTAATGGTCTTTTCTG+TGG - chr1_2:57376579-57376598 None:intergenic 40.0%
! TTTTTGCCATGTGTAGAGTG+TGG - chr1_2:57376613-57376632 None:intergenic 40.0%
AAAATGGAATATTCCCGTGC+CGG + chr1_2:57376641-57376660 Msa0079470:CDS 40.0%
!! TTGGTGTCGTTGAATGTATC+TGG - chr1_2:57376692-57376711 None:intergenic 40.0%
! CCATTAGAATTTGGAACTCC+AGG - chr1_2:57376729-57376748 None:intergenic 40.0%
CCTGGAGTTCCAAATTCTAA+TGG + chr1_2:57376726-57376745 Msa0079470:CDS 40.0%
AACCCATGACAAGAACACTA+GGG - chr1_2:57376803-57376822 None:intergenic 40.0%
TAACCCATGACAAGAACACT+AGG - chr1_2:57376804-57376823 None:intergenic 40.0%
! AAATTCACACAGAAAGCGCA+TGG + chr1_2:57376863-57376882 Msa0079470:CDS 40.0%
TCATCAAGTATCAAGAGTCC+AGG - chr1_2:57376909-57376928 None:intergenic 40.0%
TGGACTCTTGATACTTGATG+AGG + chr1_2:57376908-57376927 Msa0079470:CDS 40.0%
GGACTCTTGATACTTGATGA+GGG + chr1_2:57376909-57376928 Msa0079470:CDS 40.0%
! GAAGTACCACATCAAAGTTG+AGG + chr1_2:57376943-57376962 Msa0079470:CDS 40.0%
AGTTCTTCTGATACTCTTCC+TGG + chr1_2:57377287-57377306 Msa0079470:CDS 40.0%
! CACAATCTCGTGTTGTTCAT+CGG - chr1_2:57377345-57377364 None:intergenic 40.0%
!!! CTGCTGTTTTGATCAACCAT+GGG - chr1_2:57377448-57377467 None:intergenic 40.0%
!!! TCTGCTGTTTTGATCAACCA+TGG - chr1_2:57377449-57377468 None:intergenic 40.0%
! TGTGCAGCTAAGTTTTTCTC+CGG + chr1_2:57377473-57377492 Msa0079470:CDS 40.0%
TTAAAAACCTCACTGGTGCA+AGG - chr1_2:57377622-57377641 None:intergenic 40.0%
GAAGAACTACTTGGAAAGAG+TGG + chr1_2:57377966-57377985 Msa0079470:CDS 40.0%
TTGTTGAAGATCCTTCCGAA+TGG + chr1_2:57378017-57378036 Msa0079470:CDS 40.0%
AATCTTCTCAGCTTGCCATT+CGG - chr1_2:57378035-57378054 None:intergenic 40.0%
AGATGAAATACTCAGGGAGA+TGG + chr1_2:57378057-57378076 Msa0079470:CDS 40.0%
GCTCTTCATTGCCACAATCT+AGG + chr1_2:57374722-57374741 Msa0079470:CDS 45.0%
! GAGCATGATTATGACGGAGA+AGG + chr1_2:57374925-57374944 Msa0079470:CDS 45.0%
CCAATTTCGCTTCTTGGCAT+TGG - chr1_2:57374961-57374980 None:intergenic 45.0%
CCAATGCCAAGAAGCGAAAT+TGG + chr1_2:57374958-57374977 Msa0079470:CDS 45.0%
ATGCCAAGAAGCGAAATTGG+AGG + chr1_2:57374961-57374980 Msa0079470:CDS 45.0%
AGACCTCCAATTTCGCTTCT+TGG - chr1_2:57374967-57374986 None:intergenic 45.0%
GTTAAATGCTGATACCTGCG+AGG + chr1_2:57375245-57375264 Msa0079470:CDS 45.0%
CATATGCCAGCCAAATCATC+AGG - chr1_2:57375393-57375412 None:intergenic 45.0%
! CTGATGATTTGGCTGGCATA+TGG + chr1_2:57375391-57375410 Msa0079470:CDS 45.0%
GGAATATACTGCCGTACATG+CGG + chr1_2:57375871-57375890 Msa0079470:CDS 45.0%
! GTTTGAAATCACGGTGGATC+TGG - chr1_2:57376078-57376097 None:intergenic 45.0%
!! TTAGCCTTGTCACCTACCAT+TGG - chr1_2:57376218-57376237 None:intergenic 45.0%
GGTAGGTGACAAGGCTAAAA+TGG + chr1_2:57376220-57376239 Msa0079470:intron 45.0%
GAGAAATCTGACACAAGTGG+TGG + chr1_2:57376474-57376493 Msa0079470:CDS 45.0%
! AGAAAGGTTTTTCCAGCTCC+AGG - chr1_2:57376519-57376538 None:intergenic 45.0%
!! GTCTTTGGAGCTAGGACTAA+TGG - chr1_2:57376591-57376610 None:intergenic 45.0%
CATGTGTAGAGTGTGGTCTT+TGG - chr1_2:57376606-57376625 None:intergenic 45.0%
CAAAGACCACACTCTACACA+TGG + chr1_2:57376604-57376623 Msa0079470:CDS 45.0%
CAGCTGTGGATAAGATACAC+CGG - chr1_2:57376663-57376682 None:intergenic 45.0%
!!! CCAGGCAGAACCATTGATTT+TGG - chr1_2:57376711-57376730 None:intergenic 45.0%
! CCAAAATCAATGGTTCTGCC+TGG + chr1_2:57376708-57376727 Msa0079470:CDS 45.0%
ACACCCTAGTGTTCTTGTCA+TGG + chr1_2:57376797-57376816 Msa0079470:CDS 45.0%
CACCCTAGTGTTCTTGTCAT+GGG + chr1_2:57376798-57376817 Msa0079470:CDS 45.0%
!! AAAGCATTGAGGGAAAGTCC+TGG + chr1_2:57376888-57376907 Msa0079470:CDS 45.0%
GCACTTCCTCAACTTTGATG+TGG - chr1_2:57376952-57376971 None:intergenic 45.0%
GTACAGAAGAGGCAAACTAG+AGG + chr1_2:57377109-57377128 Msa0079470:CDS 45.0%
ACGCGCGAGAAAATTCTTCT+TGG + chr1_2:57377157-57377176 Msa0079470:CDS 45.0%
CTCAACCTCAAGAGAGTAAC+CGG - chr1_2:57377399-57377418 None:intergenic 45.0%
TGCTCATCACACGTGAGATT+AGG - chr1_2:57377571-57377590 None:intergenic 45.0%
AATCTCACGTGTGATGAGCA+AGG + chr1_2:57377571-57377590 Msa0079470:CDS 45.0%
ACGTGTGATGAGCAAGGAAA+AGG + chr1_2:57377577-57377596 Msa0079470:CDS 45.0%
TCGACCTGGTCAAGAGAAAA+TGG + chr1_2:57377892-57377911 Msa0079470:CDS 45.0%
!! CTTGTTGATGACTGGATCCA+TGG + chr1_2:57377928-57377947 Msa0079470:CDS 45.0%
!! TATATAAAAAAAATTATCAA+TGG + chr1_2:57373705-57373724 Msa0079470:intron 5.0%
TGCTCCAGTTTCCTAAGACC+TGG + chr1_2:57374253-57374272 Msa0079470:CDS 50.0%
AGATGACGCGGTAACAAACC+AGG - chr1_2:57374779-57374798 None:intergenic 50.0%
! ATGACGGAGAAGGTGCAATG+CGG + chr1_2:57374935-57374954 Msa0079470:CDS 50.0%
GTCTACGACGCTCAAAACGT+AGG + chr1_2:57374983-57375002 Msa0079470:CDS 50.0%
!! AGCATCGATTACTTCCTCGC+AGG - chr1_2:57375262-57375281 None:intergenic 50.0%
CGTATCGAAAAGCGGTGATG+AGG + chr1_2:57375359-57375378 Msa0079470:CDS 50.0%
TCAGCATTTGCACCCTGCTA+TGG - chr1_2:57375778-57375797 None:intergenic 50.0%
ATATCTCATGCTCCTGGAGC+TGG + chr1_2:57376504-57376523 Msa0079470:CDS 50.0%
!! AGAGTGTGGTCTTTGGAGCT+AGG - chr1_2:57376599-57376618 None:intergenic 50.0%
TGGATAAGATACACCGGCAC+GGG - chr1_2:57376657-57376676 None:intergenic 50.0%
GTGGATAAGATACACCGGCA+CGG - chr1_2:57376658-57376677 None:intergenic 50.0%
!! AGCGCATGGCTAAAGCATTG+AGG + chr1_2:57376877-57376896 Msa0079470:CDS 50.0%
!! GCGCATGGCTAAAGCATTGA+GGG + chr1_2:57376878-57376897 Msa0079470:CDS 50.0%
CAGAAGAGGCAAACTAGAGG+AGG + chr1_2:57377112-57377131 Msa0079470:CDS 50.0%
!! GGAAGCGCCTCATTTACTAG+AGG + chr1_2:57377133-57377152 Msa0079470:CDS 50.0%
!! AAGAAAATGGCGGGCTGTAC+CGG + chr1_2:57377377-57377396 Msa0079470:CDS 50.0%
!! CTGTACCGGTTACTCTCTTG+AGG + chr1_2:57377391-57377410 Msa0079470:CDS 50.0%
!! GTTGATGACTGGATCCATGG+AGG + chr1_2:57377931-57377950 Msa0079470:CDS 50.0%
TTCTCAGCTTGCCATTCGGA+AGG - chr1_2:57378031-57378050 None:intergenic 50.0%
ACTCAGGGAGATGGTTGAAG+AGG + chr1_2:57378066-57378085 Msa0079470:CDS 50.0%
GAATGGCTCATGGGAACCTG+TGG + chr1_2:57374114-57374133 Msa0079470:CDS 55.0%
GCAGGGTGCAAATGCTGATG+AGG + chr1_2:57375780-57375799 Msa0079470:intron 55.0%
! GTATCTGGCTTCGACAGCTG+TGG - chr1_2:57376677-57376696 None:intergenic 55.0%
GCGCGTGCCTCTAGTAAATG+AGG - chr1_2:57377143-57377162 None:intergenic 55.0%
CACTCGGGTCAATACACCCA+TGG + chr1_2:57377429-57377448 Msa0079470:CDS 55.0%
GTCACAACCTTGCACCAGTG+AGG + chr1_2:57377612-57377631 Msa0079470:CDS 55.0%
! ATTGCACGCGCTTTTCGACC+TGG + chr1_2:57377878-57377897 Msa0079470:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 57373567 57378144 57373567 ID=Msa0079470;Name=Msa0079470
chr1_2 mRNA 57373567 57378144 57373567 ID=Msa0079470-mRNA-1;Parent=Msa0079470;Name=Msa0079470-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|0.8|1|1|5|0|1123
chr1_2 exon 57373567 57373624 57373567 ID=Msa0079470-mRNA-1:exon:18094;Parent=Msa0079470-mRNA-1
chr1_2 exon 57374098 57374336 57374098 ID=Msa0079470-mRNA-1:exon:18095;Parent=Msa0079470-mRNA-1
chr1_2 exon 57374460 57375464 57374460 ID=Msa0079470-mRNA-1:exon:18096;Parent=Msa0079470-mRNA-1
chr1_2 exon 57375784 57375933 57375784 ID=Msa0079470-mRNA-1:exon:18097;Parent=Msa0079470-mRNA-1
chr1_2 exon 57376225 57378144 57376225 ID=Msa0079470-mRNA-1:exon:18098;Parent=Msa0079470-mRNA-1
chr1_2 CDS 57373567 57373624 57373567 ID=Msa0079470-mRNA-1:cds;Parent=Msa0079470-mRNA-1
chr1_2 CDS 57374098 57374336 57374098 ID=Msa0079470-mRNA-1:cds;Parent=Msa0079470-mRNA-1
chr1_2 CDS 57374460 57375464 57374460 ID=Msa0079470-mRNA-1:cds;Parent=Msa0079470-mRNA-1
chr1_2 CDS 57375784 57375933 57375784 ID=Msa0079470-mRNA-1:cds;Parent=Msa0079470-mRNA-1
chr1_2 CDS 57376225 57378144 57376225 ID=Msa0079470-mRNA-1:cds;Parent=Msa0079470-mRNA-1
Gene Sequence

>Msa0079470

ATGGCGCCCGAAAAAAATTATTTGCATCAATCGAAACATCCATTCGATGCTAATTATCCATTTGAAGCAGTATTGAATGGCTCATGGGAACCTGTGGAACTCATAAAAATTGATTCTGGGAAAACTACCATACATTTCCTCCATGCACAACAAACTCATAGTAATTTGTCATCAGACATCCGGATAAGATCAAGAAAAGCTACTTCGACTGATTGCTCCAGTTTCCTAAGACCTGGAATTGACATATCTGTGCTATCAAGCTTTCGATACGATAAAAACTCACGTCAATCTAGCCCAATGTCGACTGATGCTAGAGTGAATTCCATACACAAAAAACCTCACAAATATAACCAATGTTCATGTCGATTTCATGTAAACTTTTATGATGATCAAGGTTCACTTGGCACTGAAATAAAAACTCTGAAGGAAGAGGATAATGTGATTGGAATTGAGAAAATTTTCATCCTCCAAAGACTTCAACGAAATAACTGTGAAGGATCGTTAAGAGAAAATGATTGTTCTGAAGTTAATTCTTTTAGAAGGGATTCATCAGAAGACTGCTCTTCATTGCCACAATCTAGGTTACTTTTCGGGAAGTTTTTGTCTGATCTTTCCTGGTTTGTTACCGCGTCATCTTTAAAGAAGGTTGCATTTGTCATAAGATCGGTTCAAAACAAGCTTGTGCATCAAGTTATGGGAAATGATCCTAATATTAGTAGTCCTATGAATGTTTTGAACTTCAAATCAGAAAACGGATCACTTGAGCATGATTATGACGGAGAAGGTGCAATGCGGCCAATGCCAAGAAGCGAAATTGGAGGTCTACGACGCTCAAAACGTAGGACCATACAACTTGAACATTACGTAGATTGTAATGATGAAAAGTTGGAAGGTGGTGATAATGTTTCTTCGGGACATGTTAATAATTTCTGCACAAAGAAGCTTAAGAGAAGGTGGTTGTTTAAGGAGCATATGGATTTGATAGCTTTATGGGAAGCGATACAATCCAAAGAAGGTGTTCAAAAGAAAACGACAAACAGTATCGATGATCATGAAGAACAGGATCATAGAGGCAGTAGAACGTTAAATGCTGATACCTGCGAGGAAGTAATCGATGCTTACATGGATAATTTTGATAGCCTACCAGAAGATCCAACTATCAGTGAAGAAAGACAGTTTGAGCAGGAGAAAGAAAACGTATCGAAAAGCGGTGATGAGGTAGAAAATCCTGATGATTTGGCTGGCATATGGGAAGAAATGAATACAGCTTTGACATCAAGTTATCTTCTTGATGGAAATGAGGGTGCAAATGCTGATGAGGTTTTAGCTGACACAAATAAAGAATCCAAGGAAACATGTGAACATGATTATCGATTGGATGAACAAATTGGAATATACTGCCGTACATGCGGCTTTGTGAAAACTGAGATACGATACATTTCTGAACCCATTGTGACAAGGCTAAAATGGTACGAACAGAAGAAACAATGCAGTGAAGATACGGAACAAGTTGATGAAGATGATAATAATGATACGTTCTCAACTGATGCTACTGATCTTGGTGAACCTATATCCAAAGAAAATGATAGTGTTTGGGAATTGATTCCTGAACTCAAAGAAAACATGCATGCACACCAGAAAAAAGCTTTCGAGTTTCTTTGGAAAAACATTGCAGGGTCTATGGATCAATCACTCATGAAAGAGAAATCTGACACAAGTGGTGGCTGTGTTATATCTCATGCTCCTGGAGCTGGAAAAACCTTTCTCATCATCTCTTTTCTTGTAAGCTATTTGAAATTATTTCCACAGAAAAGACCATTAGTCCTAGCTCCAAAGACCACACTCTACACATGGCAAAAAGAGTTCGAAAAATGGAATATTCCCGTGCCGGTGTATCTTATCCACAGCTGTCGAAGCCAGATACATTCAACGACACCAAAATCAATGGTTCTGCCTGGAGTTCCAAATTCTAATGGTGTCAAGCATGATTTGGATTGTCTAGAAAAGATCAAAAGCTGGAATTCACACCCTAGTGTTCTTGTCATGGGTTATTCTTCATTTTTAGCCTTAATGAGAACAGAAGATAAGAAAAATTCACACAGAAAGCGCATGGCTAAAGCATTGAGGGAAAGTCCTGGACTCTTGATACTTGATGAGGGACACAATCCTAGAAGTACCACATCAAAGTTGAGGAAGTGCTTAATGGATCTACCAGCAGCATTGAGAATTTTACTTTCTGGCACTTTGTTTCAAAACAATTTTGGGGAATACTTCAACACTCTCTGTTTAGCCAGACCAAAATTTATTCACGAAGTTCTGGAAGAGTTAGATTCTAAGTACAGAAGAGGCAAACTAGAGGAGGAAGCGCCTCATTTACTAGAGGCACGCGCGAGAAAATTCTTCTTGGAGAACATTGAAAAGAAAATGAACTCAAATATTGATGCGGAAAAAATGAAGGGTGTAGATGTGCTTCGAAAAATCACAAATGGTTTTATAGATGTTTATGACAGTGCAAGTTCTTCTGATACTCTTCCTGGTTTGCAAATCTATACATTGCTCGTGAATGCATCCGATGAACAACACGAGATTGTGCAGAAACTGCAAAAGAAAATGGCGGGCTGTACCGGTTACTCTCTTGAGGTTGAGCTTTTGATAACACTCGGGTCAATACACCCATGGTTGATCAAAACAGCAGAAACATGTGCAGCTAAGTTTTTCTCCGGTGAAGAGTTGAAAAAACTAGACGAAAACAAATTTGCTTTGAGAAAAGGTTCCAAAGTAAGATTTGTTTTGAGCCTAATCTCACGTGTGATGAGCAAGGAAAAGGTTCTTATCTTCTGTCACAACCTTGCACCAGTGAGGTTTTTAATAGAGTTATTTGAGAATCATTTTCAGTGGAAAAATGGTAAAGAGATTCTGCAACTAACTGGTGAACAAGACTTCTTTGAAAGAACGAATGTGATAGATAAATTTGAAGATCGTTGTGGCGATTCAAAGATACTTCTTGCTTCAATCAATGCTTGTTCTGAAGGTATCAGTTTAACATCAGCTTCAAGAGTGATTTTCTTGGATTCTGAGTGGAATCCTTCAAAAACAAAGCAAGCTATTGCACGCGCTTTTCGACCTGGTCAAGAGAAAATGGTTTATGTATATCACTTGTTGATGACTGGATCCATGGAGGAAGATAAATATAGAAGAACTACTTGGAAAGAGTGGGTTTCTTGTATGATTTTCAGTGAGGAGCTTGTTGAAGATCCTTCCGAATGGCAAGCTGAGAAGATTGAAGATGAAATACTCAGGGAGATGGTTGAAGAGGACAAGTCTAAACTCATTCATATGATTATGAAGAATGAAAAGACTTCAACAAGCTAA
Protein sequence

>Msa0079470

MAPEKNYLHQSKHPFDANYPFEAVLNGSWEPVELIKIDSGKTTIHFLHAQQTHSNLSSDIRIRSRKATSTDCSSFLRPGIDISVLSSFRYDKNSRQSSPMSTDARVNSIHKKPHKYNQCSCRFHVNFYDDQGSLGTEIKTLKEEDNVIGIEKIFILQRLQRNNCEGSLRENDCSEVNSFRRDSSEDCSSLPQSRLLFGKFLSDLSWFVTASSLKKVAFVIRSVQNKLVHQVMGNDPNISSPMNVLNFKSENGSLEHDYDGEGAMRPMPRSEIGGLRRSKRRTIQLEHYVDCNDEKLEGGDNVSSGHVNNFCTKKLKRRWLFKEHMDLIALWEAIQSKEGVQKKTTNSIDDHEEQDHRGSRTLNADTCEEVIDAYMDNFDSLPEDPTISEERQFEQEKENVSKSGDEVENPDDLAGIWEEMNTALTSSYLLDGNEGANADEVLADTNKESKETCEHDYRLDEQIGIYCRTCGFVKTEIRYISEPIVTRLKWYEQKKQCSEDTEQVDEDDNNDTFSTDATDLGEPISKENDSVWELIPELKENMHAHQKKAFEFLWKNIAGSMDQSLMKEKSDTSGGCVISHAPGAGKTFLIISFLVSYLKLFPQKRPLVLAPKTTLYTWQKEFEKWNIPVPVYLIHSCRSQIHSTTPKSMVLPGVPNSNGVKHDLDCLEKIKSWNSHPSVLVMGYSSFLALMRTEDKKNSHRKRMAKALRESPGLLILDEGHNPRSTTSKLRKCLMDLPAALRILLSGTLFQNNFGEYFNTLCLARPKFIHEVLEELDSKYRRGKLEEEAPHLLEARARKFFLENIEKKMNSNIDAEKMKGVDVLRKITNGFIDVYDSASSSDTLPGLQIYTLLVNASDEQHEIVQKLQKKMAGCTGYSLEVELLITLGSIHPWLIKTAETCAAKFFSGEELKKLDENKFALRKGSKVRFVLSLISRVMSKEKVLIFCHNLAPVRFLIELFENHFQWKNGKEILQLTGEQDFFERTNVIDKFEDRCGDSKILLASINACSEGISLTSASRVIFLDSEWNPSKTKQAIARAFRPGQEKMVYVYHLLMTGSMEEDKYRRTTWKEWVSCMIFSEELVEDPSEWQAEKIEDEILREMVEEDKSKLIHMIMKNEKTSTS*