AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0080630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0080630 MtrunA17_Chr1g0192841 83.402 241 26 1 164 390 3 243 1.29e-132 379
Msa0080630 MtrunA17_Chr5g0435401 48.214 392 147 10 32 390 44 412 4.07e-97 295
Msa0080630 MtrunA17_Chr5g0445971 59.394 165 62 3 2 166 5 164 1.51e-55 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCGTTGGATATGTTAAA+TGG 0.182684 1_2:+58919389 Msa0080630:CDS
GGTGCTTATGCTGCAATATT+TGG 0.183459 1_2:+58918712 Msa0080630:CDS
TACTGGTTTATCTCGATTAA+TGG 0.250811 1_2:+58918346 Msa0080630:CDS
TCCCTTCTTCCCAGTTGATT+TGG 0.271998 1_2:+58919358 Msa0080630:CDS
CCTTCGGTGCTGCCGGTTAC+AGG 0.272596 1_2:-58917965 None:intergenic
AGTTGTCTCTGCACTGAAAT+TGG 0.295292 1_2:+58917853 Msa0080630:CDS
GATTTGTTAAATGAGTTTAA+AGG 0.306346 1_2:+58918670 Msa0080630:CDS
TGTCAAACTAACTGAAGTTT+TGG 0.307954 1_2:+58916821 Msa0080630:CDS
AACAGTACCGTTGGTGAGTT+TGG 0.309542 1_2:-58916763 None:intergenic
TTCTTGCTTTCCTCCACATA+TGG 0.365771 1_2:-58919107 None:intergenic
TTTGTAAAGGTACGCGTAAA+AGG 0.366190 1_2:+58918309 Msa0080630:intron
TTCAGTTAGTTTGACATAGA+AGG 0.372838 1_2:-58916814 None:intergenic
CAGTAAGTATCCAAATCAAC+TGG 0.378693 1_2:-58919368 None:intergenic
TTCTACAGGTTTCCTGTAAC+CGG 0.383374 1_2:+58917953 Msa0080630:CDS
GAAGAGCACTCAAGATCTTA+AGG 0.392322 1_2:+58919208 Msa0080630:CDS
AGGTTGGTCTTCAAAACAAA+TGG 0.395740 1_2:+58917825 Msa0080630:intron
TTGAGAAATTGTACTTCTAC+AGG 0.399495 1_2:+58917939 Msa0080630:CDS
GGTGTTTCTTGTGACTTTAG+AGG 0.401222 1_2:+58918691 Msa0080630:CDS
AATCCTTATAACCTGTCATT+TGG 0.408344 1_2:-58918418 None:intergenic
CAACCGCTAGTATAGTCATT+TGG 0.413628 1_2:-58919417 None:intergenic
CTCTATCTCTAAGTCATCAT+TGG 0.415656 1_2:-58916790 None:intergenic
GGAGGAAAGCAAGAAGATAA+AGG 0.418994 1_2:+58919115 Msa0080630:CDS
TGGTGACTTCCAAGTAAAAC+AGG 0.431064 1_2:-58917528 None:intergenic
AATGGAAGGCAACAAGACTA+TGG 0.438680 1_2:+58916716 None:intergenic
CTGGTGAAAACCTCCTCTTC+TGG 0.440306 1_2:-58917548 None:intergenic
GCTCATGATCCAGACATTGA+TGG 0.444797 1_2:+58918907 Msa0080630:CDS
ATCCAAATCAACTGGGAAGA+AGG 0.454997 1_2:-58919360 None:intergenic
GTTCGTTGGATATGTTAAAT+GGG 0.459203 1_2:+58919390 Msa0080630:CDS
TACGCATGGCTGTTGATGCT+TGG 0.460942 1_2:+58918941 Msa0080630:CDS
AGCCAGCTGTCAAGAACCTT+GGG 0.462711 1_2:+58918753 Msa0080630:CDS
TTTGGATTCTTATTTAAAGC+AGG 0.465618 1_2:+58918730 Msa0080630:CDS
AAGACAAAGAAAAGAAGAAA+CGG 0.466286 1_2:+58918818 Msa0080630:CDS
TATTTGATATCCACTTTGTC+CGG 0.466627 1_2:-58918854 None:intergenic
CACCCAAGGTTCTTGACAGC+TGG 0.474988 1_2:-58918755 None:intergenic
TTTGGATACTTACTGTTCGT+TGG 0.489477 1_2:+58919376 Msa0080630:CDS
TTAGATACCTTCGGTGCTGC+CGG 0.491330 1_2:-58917972 None:intergenic
ACAGAAGAATAGTTTGGTAG+TGG 0.492096 1_2:-58919281 None:intergenic
AGGAAGCAGAACTCGACTAC+TGG 0.510036 1_2:+58918329 Msa0080630:CDS
GATGTCTGCCACTGATAAAG+AGG 0.517133 1_2:+58918969 Msa0080630:CDS
GAGCCAGCTGTCAAGAACCT+TGG 0.520532 1_2:+58918752 Msa0080630:CDS
CAACAATGCAGCTGTGGAGT+TGG 0.523389 1_2:+58919304 Msa0080630:CDS
TTGTTGACAGAAGAATAGTT+TGG 0.526138 1_2:-58919287 None:intergenic
TCCAAATCAACTGGGAAGAA+GGG 0.526508 1_2:-58919359 None:intergenic
GAATGTGGCAAAGGTGTCCA+AGG 0.526713 1_2:+58918385 Msa0080630:CDS
TTCTGTCAACAATGCAGCTG+TGG 0.527890 1_2:+58919298 Msa0080630:CDS
TACTTGGAAGTCACCAGAAG+AGG 0.540823 1_2:+58917535 Msa0080630:CDS
GGTTGGTCTTCAAAACAAAT+GGG 0.543380 1_2:+58917826 Msa0080630:intron
GAGCACTGAAGATCTTAAGA+AGG 0.550700 1_2:+58919178 Msa0080630:CDS
TGATATCCACTTTGTCCGGT+AGG 0.555314 1_2:-58918850 None:intergenic
GGCTACTCTAGCCAAATGAC+AGG 0.557780 1_2:+58918407 Msa0080630:CDS
CCTGTAACCGGCAGCACCGA+AGG 0.559034 1_2:+58917965 Msa0080630:CDS
CATTTGGCTAGAGTAGCCCT+TGG 0.560486 1_2:-58918402 None:intergenic
GTCTCTGCACTGAAATTGGA+AGG 0.566571 1_2:+58917857 Msa0080630:CDS
AAGATCTTAAGGAGGAAAAG+AGG 0.571062 1_2:+58919219 Msa0080630:CDS
AAACGGCGAGGTCCTCCTAC+CGG 0.572639 1_2:+58918835 Msa0080630:CDS
TTCCTAGCTCAAACAATGGA+AGG 0.574513 1_2:+58916702 None:intergenic
TAGTCGTAACCGCACACACT+AGG 0.575038 1_2:-58919242 None:intergenic
TTGGAAGTCACCAGAAGAGG+AGG 0.590853 1_2:+58917538 Msa0080630:CDS
GTATCTGAAGGTGATGATGA+TGG 0.592975 1_2:-58916741 None:intergenic
TATCCACTTTGTCCGGTAGG+AGG 0.594215 1_2:-58918847 None:intergenic
TTATCATACCTCTTTATCAG+TGG 0.622643 1_2:-58918977 None:intergenic
GGTCCTCCTACCGGACAAAG+TGG 0.623840 1_2:+58918844 Msa0080630:CDS
GGTGCTTGTAGAAGCACCCA+AGG 0.627728 1_2:-58918769 None:intergenic
ACAAGTCTAAGATTGTCTCT+CGG 0.631739 1_2:-58917504 None:intergenic
TGTACTTATTAGCTGCAATG+TGG 0.632646 1_2:+58918620 Msa0080630:intron
ATTGGACACAACAGTACCGT+TGG 0.633026 1_2:-58916772 None:intergenic
GAAAAGAGGCCTAGTGTGTG+CGG 0.639000 1_2:+58919233 Msa0080630:CDS
TTGGTGAGTTTGGTATCTGA+AGG 0.656599 1_2:-58916753 None:intergenic
AGATAATCTGAATGTGGCAA+AGG 0.661885 1_2:+58918376 Msa0080630:CDS
AGTAAGTATCCAAATCAACT+GGG 0.663991 1_2:-58919367 None:intergenic
GAGCACTCAAGATCTTAAGG+AGG 0.665195 1_2:+58919211 Msa0080630:CDS
AATGTGGCAAAGGTGTCCAA+GGG 0.667130 1_2:+58918386 Msa0080630:CDS
TCATGAAGATAATCTGAATG+TGG 0.680646 1_2:+58918370 Msa0080630:CDS
AAAGAAAAGAAGAAACGGCG+AGG 0.681943 1_2:+58918823 Msa0080630:CDS
TCAGATACCAAACTCACCAA+CGG 0.688518 1_2:+58916756 Msa0080630:CDS
AGACATGAGATTGAACCCAG+AGG 0.774697 1_2:-58916847 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAAAAGTAATTATAAA+TGG - chr1_2:58919084-58919103 None:intergenic 10.0%
!!! TAAAGTTTTATGAGATTATT+TGG + chr1_2:58917405-58917424 Msa0080630:intron 15.0%
!! ATTTGGATAAAAAAAAATGA+TGG + chr1_2:58917422-58917441 Msa0080630:intron 15.0%
!!! AAAAAAATGATGGATAATAA+AGG + chr1_2:58917432-58917451 Msa0080630:intron 15.0%
!!! AAAAAATGATGGATAATAAA+GGG + chr1_2:58917433-58917452 Msa0080630:intron 15.0%
!!! TTTTATTACGTTTTTGTTTT+AGG + chr1_2:58918230-58918249 Msa0080630:intron 15.0%
!! TTGCAAAAATATAAATTGTT+CGG - chr1_2:58919031-58919050 None:intergenic 15.0%
!!! TTTTTCTTTTATTGCATGTT+TGG - chr1_2:58917129-58917148 None:intergenic 20.0%
!! TACTTGTGGATAAAAATATT+TGG + chr1_2:58917163-58917182 Msa0080630:intron 20.0%
!!! TATAACACATTTCAGTTTTA+AGG - chr1_2:58917297-58917316 None:intergenic 20.0%
!! TTTATTCCTCTTTAAATGTT+TGG + chr1_2:58917318-58917337 Msa0080630:intron 20.0%
!! TAATACAAATTAGTCAATAG+AGG + chr1_2:58917730-58917749 Msa0080630:intron 20.0%
!!! GATTTGTTAAATGAGTTTAA+AGG + chr1_2:58918670-58918689 Msa0080630:CDS 20.0%
!! GATAAAAATATTTGGTTCGA+GGG + chr1_2:58917171-58917190 Msa0080630:intron 25.0%
!! ATAAAAATATTTGGTTCGAG+GGG + chr1_2:58917172-58917191 Msa0080630:intron 25.0%
!! ATGTCTCAATTTTTCTTTTG+TGG - chr1_2:58917256-58917275 None:intergenic 25.0%
! CTTATACCAAACATTTAAAG+AGG - chr1_2:58917327-58917346 None:intergenic 25.0%
! CTCTTTAAATGTTTGGTATA+AGG + chr1_2:58917325-58917344 Msa0080630:intron 25.0%
! AGAACAAATTAAATCATGTG+AGG - chr1_2:58917601-58917620 None:intergenic 25.0%
! TATAGACACAACATATTTGA+TGG - chr1_2:58917710-58917729 None:intergenic 25.0%
! ATGCTTGATATGTAAATGTT+TGG + chr1_2:58918065-58918084 Msa0080630:intron 25.0%
! AAAATGCCTTGTAAAAACAT+TGG + chr1_2:58918131-58918150 Msa0080630:intron 25.0%
!! ATTGGTCTAAGAAAATGTAT+TGG + chr1_2:58918149-58918168 Msa0080630:intron 25.0%
!! ATAAAGTAACAAACCTGTTT+TGG - chr1_2:58918601-58918620 None:intergenic 25.0%
!! TTTGGATTCTTATTTAAAGC+AGG + chr1_2:58918730-58918749 Msa0080630:CDS 25.0%
!!! TTCTTTTCTTTGTCATTTGA+GGG - chr1_2:58918793-58918812 None:intergenic 25.0%
! AAGACAAAGAAAAGAAGAAA+CGG + chr1_2:58918818-58918837 Msa0080630:CDS 25.0%
! ATTTCAAACAAACAAAGTAG+AGG - chr1_2:58919058-58919077 None:intergenic 25.0%
! GAAGATAAAGGAAAAAAAGA+AGG + chr1_2:58919127-58919146 Msa0080630:CDS 25.0%
! TTCAGTTAGTTTGACATAGA+AGG - chr1_2:58916817-58916836 None:intergenic 30.0%
! TGTCAAACTAACTGAAGTTT+TGG + chr1_2:58916821-58916840 Msa0080630:CDS 30.0%
TTGTGGCAATAACTACAAAT+TGG - chr1_2:58917093-58917112 None:intergenic 30.0%
TTGGATTGAGGATAAATTTG+TGG - chr1_2:58917110-58917129 None:intergenic 30.0%
TTTATTGCATGTTTGGATTG+AGG - chr1_2:58917122-58917141 None:intergenic 30.0%
! GGATAAAAATATTTGGTTCG+AGG + chr1_2:58917170-58917189 Msa0080630:intron 30.0%
! TCTCAATTTTTCTTTTGTGG+TGG - chr1_2:58917253-58917272 None:intergenic 30.0%
AGACATGCATTTAGTCATAT+TGG + chr1_2:58917270-58917289 Msa0080630:intron 30.0%
! ACATATTTGATGGATAGACT+TGG - chr1_2:58917700-58917719 None:intergenic 30.0%
TTGAGAAATTGTACTTCTAC+AGG + chr1_2:58917939-58917958 Msa0080630:CDS 30.0%
ATTGATCTCTTAGATACCTT+CGG - chr1_2:58917984-58918003 None:intergenic 30.0%
GTATCTAAGAGATCAATTTG+TGG + chr1_2:58917987-58918006 Msa0080630:intron 30.0%
! ATATCGATCTTGTTTCATTC+CGG + chr1_2:58918023-58918042 Msa0080630:intron 30.0%
!! CTTAGACCAATGTTTTTACA+AGG - chr1_2:58918140-58918159 None:intergenic 30.0%
GGATGTGAAATTAAATCACT+CGG + chr1_2:58918170-58918189 Msa0080630:intron 30.0%
AGAAAAGAAAGGAGACTTTA+AGG - chr1_2:58918275-58918294 None:intergenic 30.0%
!! TACTGGTTTATCTCGATTAA+TGG + chr1_2:58918346-58918365 Msa0080630:CDS 30.0%
TCATGAAGATAATCTGAATG+TGG + chr1_2:58918370-58918389 Msa0080630:CDS 30.0%
AATCCTTATAACCTGTCATT+TGG - chr1_2:58918421-58918440 None:intergenic 30.0%
! ATTCACTGATTGAATGCAAA+AGG + chr1_2:58918467-58918486 Msa0080630:intron 30.0%
GATTACTAAAGTTCCAAAAC+AGG + chr1_2:58918585-58918604 Msa0080630:intron 30.0%
!! CTTCTTTTCTTTGTCATTTG+AGG - chr1_2:58918794-58918813 None:intergenic 30.0%
TATTTGATATCCACTTTGTC+CGG - chr1_2:58918857-58918876 None:intergenic 30.0%
TTATCATACCTCTTTATCAG+TGG - chr1_2:58918980-58918999 None:intergenic 30.0%
!!! CTTTTTTATGTAGCCATATG+TGG + chr1_2:58919094-58919113 Msa0080630:intron 30.0%
TTGTTGACAGAAGAATAGTT+TGG - chr1_2:58919290-58919309 None:intergenic 30.0%
AGTAAGTATCCAAATCAACT+GGG - chr1_2:58919370-58919389 None:intergenic 30.0%
TGTTCGTTGGATATGTTAAA+TGG + chr1_2:58919389-58919408 Msa0080630:CDS 30.0%
GTTCGTTGGATATGTTAAAT+GGG + chr1_2:58919390-58919409 Msa0080630:CDS 30.0%
CTCTATCTCTAAGTCATCAT+TGG - chr1_2:58916793-58916812 None:intergenic 35.0%
TGTGTGCATTATGCTATGTT+TGG + chr1_2:58916944-58916963 Msa0080630:intron 35.0%
TTCCTGCATGTTTGGATATT+TGG + chr1_2:58917018-58917037 Msa0080630:intron 35.0%
ATCCAAATATCCAAACATGC+AGG - chr1_2:58917023-58917042 None:intergenic 35.0%
! GAAAAAAGTGTGTGTACTTG+TGG + chr1_2:58917149-58917168 Msa0080630:intron 35.0%
ACTAGCTAGTGACCTTTATT+GGG - chr1_2:58917202-58917221 None:intergenic 35.0%
AATGTTTGGTATAAGGTCAC+TGG + chr1_2:58917332-58917351 Msa0080630:intron 35.0%
AGAATTGAAACAGAGTTGCA+TGG - chr1_2:58917466-58917485 None:intergenic 35.0%
! ACAAGTCTAAGATTGTCTCT+CGG - chr1_2:58917507-58917526 None:intergenic 35.0%
! AGACTTGTACCTGTTTTACT+TGG + chr1_2:58917519-58917538 Msa0080630:CDS 35.0%
TGAGGTCAATATGTGGATAA+CGG - chr1_2:58917583-58917602 None:intergenic 35.0%
AATCATGTGAGGTCAATATG+TGG - chr1_2:58917590-58917609 None:intergenic 35.0%
!!! ATTTGTTTTGAAGACCAACC+TGG - chr1_2:58917826-58917845 None:intergenic 35.0%
!! AGGTTGGTCTTCAAAACAAA+TGG + chr1_2:58917825-58917844 Msa0080630:intron 35.0%
!! GGTTGGTCTTCAAAACAAAT+GGG + chr1_2:58917826-58917845 Msa0080630:intron 35.0%
ACTGCATATAAGTGAAGTAC+CGG - chr1_2:58918045-58918064 None:intergenic 35.0%
TGTGAAATTAAATCACTCGG+TGG + chr1_2:58918173-58918192 Msa0080630:intron 35.0%
CTTTACAAAATGCAAGTGTG+AGG - chr1_2:58918298-58918317 None:intergenic 35.0%
! CTCACACTTGCATTTTGTAA+AGG + chr1_2:58918296-58918315 Msa0080630:intron 35.0%
! TTTGTAAAGGTACGCGTAAA+AGG + chr1_2:58918309-58918328 Msa0080630:intron 35.0%
AGATAATCTGAATGTGGCAA+AGG + chr1_2:58918376-58918395 Msa0080630:CDS 35.0%
TAGCCAAATGACAGGTTATA+AGG + chr1_2:58918415-58918434 Msa0080630:intron 35.0%
TGTACTTATTAGCTGCAATG+TGG + chr1_2:58918620-58918639 Msa0080630:intron 35.0%
!!! ACTTTTTTGCCATCAATGTC+TGG - chr1_2:58918919-58918938 None:intergenic 35.0%
! TTTTATGTAGCCATATGTGG+AGG + chr1_2:58919097-58919116 Msa0080630:intron 35.0%
AAGATCTTAAGGAGGAAAAG+AGG + chr1_2:58919219-58919238 Msa0080630:CDS 35.0%
ACAGAAGAATAGTTTGGTAG+TGG - chr1_2:58919284-58919303 None:intergenic 35.0%
!! AATGACTGAAAAAGCACTCA+AGG + chr1_2:58919334-58919353 Msa0080630:CDS 35.0%
CAGTAAGTATCCAAATCAAC+TGG - chr1_2:58919371-58919390 None:intergenic 35.0%
TTTGGATACTTACTGTTCGT+TGG + chr1_2:58919376-58919395 Msa0080630:CDS 35.0%
! GTATCTGAAGGTGATGATGA+TGG - chr1_2:58916744-58916763 None:intergenic 40.0%
TTGGTGAGTTTGGTATCTGA+AGG - chr1_2:58916756-58916775 None:intergenic 40.0%
TCAGATACCAAACTCACCAA+CGG + chr1_2:58916756-58916775 Msa0080630:CDS 40.0%
ATATCCAAACATGCAGGAAG+TGG - chr1_2:58917017-58917036 None:intergenic 40.0%
CTAGCTAGTGACCTTTATTG+GGG - chr1_2:58917201-58917220 None:intergenic 40.0%
! GACTAGCTAGTGACCTTTAT+TGG - chr1_2:58917203-58917222 None:intergenic 40.0%
! CGTTCTCAGAGATTAGTCAT+CGG + chr1_2:58917222-58917241 Msa0080630:intron 40.0%
TGGTGACTTCCAAGTAAAAC+AGG - chr1_2:58917531-58917550 None:intergenic 40.0%
GAGGTCAATATGTGGATAAC+GGG - chr1_2:58917582-58917601 None:intergenic 40.0%
TGATTCTCTTGAACTTGTCC+AGG + chr1_2:58917805-58917824 Msa0080630:intron 40.0%
AGTTGTCTCTGCACTGAAAT+TGG + chr1_2:58917853-58917872 Msa0080630:CDS 40.0%
TTCTACAGGTTTCCTGTAAC+CGG + chr1_2:58917953-58917972 Msa0080630:CDS 40.0%
! GTGGTGACACAAGTTTTCAT+CGG + chr1_2:58918192-58918211 Msa0080630:intron 40.0%
CAAGTGTGAGGAGAAAAGAA+AGG - chr1_2:58918286-58918305 None:intergenic 40.0%
GGTGTTTCTTGTGACTTTAG+AGG + chr1_2:58918691-58918710 Msa0080630:CDS 40.0%
GGTGCTTATGCTGCAATATT+TGG + chr1_2:58918712-58918731 Msa0080630:CDS 40.0%
AAAGAAAAGAAGAAACGGCG+AGG + chr1_2:58918823-58918842 Msa0080630:CDS 40.0%
TGGCAAAAAAGTTCTACGCA+TGG + chr1_2:58918927-58918946 Msa0080630:CDS 40.0%
TTCTTGCTTTCCTCCACATA+TGG - chr1_2:58919110-58919129 None:intergenic 40.0%
GGAGGAAAGCAAGAAGATAA+AGG + chr1_2:58919115-58919134 Msa0080630:CDS 40.0%
!! GAGCACTGAAGATCTTAAGA+AGG + chr1_2:58919178-58919197 Msa0080630:CDS 40.0%
!! GAAGAGCACTCAAGATCTTA+AGG + chr1_2:58919208-58919227 Msa0080630:CDS 40.0%
TCCAAATCAACTGGGAAGAA+GGG - chr1_2:58919362-58919381 None:intergenic 40.0%
ATCCAAATCAACTGGGAAGA+AGG - chr1_2:58919363-58919382 None:intergenic 40.0%
! AACAGTACCGTTGGTGAGTT+TGG - chr1_2:58916766-58916785 None:intergenic 45.0%
ATTGGACACAACAGTACCGT+TGG - chr1_2:58916775-58916794 None:intergenic 45.0%
!!! ACTGAAGTTTTGGACTCCTC+TGG + chr1_2:58916831-58916850 Msa0080630:CDS 45.0%
!!! CTGAAGTTTTGGACTCCTCT+GGG + chr1_2:58916832-58916851 Msa0080630:CDS 45.0%
AGACATGAGATTGAACCCAG+AGG - chr1_2:58916850-58916869 None:intergenic 45.0%
! ATTGCCACTTCCTGCATGTT+TGG + chr1_2:58917010-58917029 Msa0080630:intron 45.0%
TACTTGGAAGTCACCAGAAG+AGG + chr1_2:58917535-58917554 Msa0080630:CDS 45.0%
!! TGGATAACGGGACAAATACC+TGG - chr1_2:58917570-58917589 None:intergenic 45.0%
TCTCTTGAACTTGTCCAGGT+TGG + chr1_2:58917809-58917828 Msa0080630:intron 45.0%
GTCTCTGCACTGAAATTGGA+AGG + chr1_2:58917857-58917876 Msa0080630:CDS 45.0%
!! AATGTGGCAAAGGTGTCCAA+GGG + chr1_2:58918386-58918405 Msa0080630:CDS 45.0%
TGATATCCACTTTGTCCGGT+AGG - chr1_2:58918853-58918872 None:intergenic 45.0%
GCTCATGATCCAGACATTGA+TGG + chr1_2:58918907-58918926 Msa0080630:CDS 45.0%
GATGTCTGCCACTGATAAAG+AGG + chr1_2:58918969-58918988 Msa0080630:CDS 45.0%
GAGCACTCAAGATCTTAAGG+AGG + chr1_2:58919211-58919230 Msa0080630:CDS 45.0%
TTCTGTCAACAATGCAGCTG+TGG + chr1_2:58919298-58919317 Msa0080630:CDS 45.0%
TCCCTTCTTCCCAGTTGATT+TGG + chr1_2:58919358-58919377 Msa0080630:CDS 45.0%
TTGGAAGTCACCAGAAGAGG+AGG + chr1_2:58917538-58917557 Msa0080630:CDS 50.0%
CTGGTGAAAACCTCCTCTTC+TGG - chr1_2:58917551-58917570 None:intergenic 50.0%
! CAGAAGAGGAGGTTTTCACC+AGG + chr1_2:58917549-58917568 Msa0080630:CDS 50.0%
!! TTAGATACCTTCGGTGCTGC+CGG - chr1_2:58917975-58917994 None:intergenic 50.0%
AGGAAGCAGAACTCGACTAC+TGG + chr1_2:58918329-58918348 Msa0080630:CDS 50.0%
!! GAATGTGGCAAAGGTGTCCA+AGG + chr1_2:58918385-58918404 Msa0080630:CDS 50.0%
CATTTGGCTAGAGTAGCCCT+TGG - chr1_2:58918405-58918424 None:intergenic 50.0%
GGCTACTCTAGCCAAATGAC+AGG + chr1_2:58918407-58918426 Msa0080630:CDS 50.0%
AGCCAGCTGTCAAGAACCTT+GGG + chr1_2:58918753-58918772 Msa0080630:CDS 50.0%
TATCCACTTTGTCCGGTAGG+AGG - chr1_2:58918850-58918869 None:intergenic 50.0%
TACGCATGGCTGTTGATGCT+TGG + chr1_2:58918941-58918960 Msa0080630:CDS 50.0%
GAAAAGAGGCCTAGTGTGTG+CGG + chr1_2:58919233-58919252 Msa0080630:CDS 50.0%
TAGTCGTAACCGCACACACT+AGG - chr1_2:58919245-58919264 None:intergenic 50.0%
CAACAATGCAGCTGTGGAGT+TGG + chr1_2:58919304-58919323 Msa0080630:CDS 50.0%
TCGAGGGGAGACCCCAATAA+AGG + chr1_2:58917187-58917206 Msa0080630:intron 55.0%
GAGCCAGCTGTCAAGAACCT+TGG + chr1_2:58918752-58918771 Msa0080630:CDS 55.0%
CACCCAAGGTTCTTGACAGC+TGG - chr1_2:58918758-58918777 None:intergenic 55.0%
!! GGTGCTTGTAGAAGCACCCA+AGG - chr1_2:58918772-58918791 None:intergenic 55.0%
AAACGGCGAGGTCCTCCTAC+CGG + chr1_2:58918835-58918854 Msa0080630:CDS 60.0%
GGTCCTCCTACCGGACAAAG+TGG + chr1_2:58918844-58918863 Msa0080630:CDS 60.0%
!! CCTTCGGTGCTGCCGGTTAC+AGG - chr1_2:58917968-58917987 None:intergenic 65.0%
! CCTGTAACCGGCAGCACCGA+AGG + chr1_2:58917965-58917984 Msa0080630:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 58916717 58919424 58916717 ID=Msa0080630;Name=Msa0080630
chr1_2 mRNA 58916717 58919424 58916717 ID=Msa0080630-mRNA-1;Parent=Msa0080630;Name=Msa0080630-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|0.83|1|1|6|0|390
chr1_2 exon 58916717 58916865 58916717 ID=Msa0080630-mRNA-1:exon:19016;Parent=Msa0080630-mRNA-1
chr1_2 exon 58917495 58917570 58917495 ID=Msa0080630-mRNA-1:exon:19017;Parent=Msa0080630-mRNA-1
chr1_2 exon 58917827 58917986 58917827 ID=Msa0080630-mRNA-1:exon:19018;Parent=Msa0080630-mRNA-1
chr1_2 exon 58918318 58918428 58918318 ID=Msa0080630-mRNA-1:exon:19019;Parent=Msa0080630-mRNA-1
chr1_2 exon 58918632 58918990 58918632 ID=Msa0080630-mRNA-1:exon:19020;Parent=Msa0080630-mRNA-1
chr1_2 exon 58919107 58919424 58919107 ID=Msa0080630-mRNA-1:exon:19021;Parent=Msa0080630-mRNA-1
chr1_2 CDS 58916717 58916865 58916717 ID=Msa0080630-mRNA-1:cds;Parent=Msa0080630-mRNA-1
chr1_2 CDS 58917495 58917570 58917495 ID=Msa0080630-mRNA-1:cds;Parent=Msa0080630-mRNA-1
chr1_2 CDS 58917827 58917986 58917827 ID=Msa0080630-mRNA-1:cds;Parent=Msa0080630-mRNA-1
chr1_2 CDS 58918318 58918428 58918318 ID=Msa0080630-mRNA-1:cds;Parent=Msa0080630-mRNA-1
chr1_2 CDS 58918632 58918990 58918632 ID=Msa0080630-mRNA-1:cds;Parent=Msa0080630-mRNA-1
chr1_2 CDS 58919107 58919424 58919107 ID=Msa0080630-mRNA-1:cds;Parent=Msa0080630-mRNA-1
Gene Sequence

>Msa0080630

ATGGAAGGCAACAAGACTATGGTTCCATCATCATCACCTTCAGATACCAAACTCACCAACGGTACTGTTGTGTCCAATGATGACTTAGAGATAGAGACCTTCTATGTCAAACTAACTGAAGTTTTGGACTCCTCTGGGTTCAATCTCATTTTCAATGTCCGAGAGACAATCTTAGACTTGTACCTGTTTTACTTGGAAGTCACCAGAAGAGGAGGTTTTCACCAGGTTGGTCTTCAAAACAAATGGGCTGAAGTTGTCTCTGCACTGAAATTGGAAGGCAGCAATGCAATGTTATCTGCTCAAGTTGAAAAGATCTATGCAACACTTCTATACAAATTTGAGAAATTGTACTTCTACAGGTTTCCTGTAACCGGCAGCACCGAAGGTACGCGTAAAAGGAAGCAGAACTCGACTACTGGTTTATCTCGATTAATGGCTCATGAAGATAATCTGAATGTGGCAAAGGTGTCCAAGGGCTACTCTAGCCAAATGACAGCTGCAATGTGGACATTTGTTTTCTTTAGTAACTCAAGTGATTTGTTAAATGAGTTTAAAGGTGTTTCTTGTGACTTTAGAGGTGCTTATGCTGCAATATTTGGATTCTTATTTAAAGCAGGAGCCAGCTGTCAAGAACCTTGGGTGCTTCTACAAGCACCCTCAAATGACAAAGAAAAGAAGAAACAAGACAAAGAAAAGAAGAAACGGCGAGGTCCTCCTACCGGACAAAGTGGATATCAAATATTTCTCAAGCATGAATGTGCTCGATTAAAAGCTCATGATCCAGACATTGATGGCAAAAAAGTTCTACGCATGGCTGTTGATGCTTGGCAGAAGATGTCTGCCACTGATAAAGAGCCATATGTGGAGGAAAGCAAGAAGATAAAGGAAAAAAAGAAGGAAGCAGTGATCATTGACAATAAACAGATGAGCACTGAAGATCTTAAGAAGGATGAAAAGAAGAGCACTCAAGATCTTAAGGAGGAAAAGAGGCCTAGTGTGTGCGGTTACGACTACTGTGCAACTTCGCAACCACTACCAAACTATTCTTCTGTCAACAATGCAGCTGTGGAGTTGGCTTTGAAAATGACTGAAAAAGCACTCAAGGATCCCTTCTTCCCAGTTGATTTGGATACTTACTGTTCGTTGGATATGTTAAATGGGAAATCCAAATGA
Protein sequence

>Msa0080630

MEGNKTMVPSSSPSDTKLTNGTVVSNDDLEIETFYVKLTEVLDSSGFNLIFNVRETILDLYLFYLEVTRRGGFHQVGLQNKWAEVVSALKLEGSNAMLSAQVEKIYATLLYKFEKLYFYRFPVTGSTEGTRKRKQNSTTGLSRLMAHEDNLNVAKVSKGYSSQMTAAMWTFVFFSNSSDLLNEFKGVSCDFRGAYAAIFGFLFKAGASCQEPWVLLQAPSNDKEKKKQDKEKKKRRGPPTGQSGYQIFLKHECARLKAHDPDIDGKKVLRMAVDAWQKMSATDKEPYVEESKKIKEKKKEAVIIDNKQMSTEDLKKDEKKSTQDLKEEKRPSVCGYDYCATSQPLPNYSSVNNAAVELALKMTEKALKDPFFPVDLDTYCSLDMLNGKSK*