AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0080790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0080790 MtrunA17_Chr1g0193071 87.500 128 10 2 1 128 1 122 7.09e-71 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0080790 AT5G26749.1 40.136 147 69 2 1 128 1 147 8.73e-32 110
Msa0080790 AT5G26749.2 35.542 166 69 3 1 128 1 166 3.05e-27 99.4

Find 84 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCTGGCATTCTTGTTAT+TGG 0.169245 1_2:+59173094 None:intergenic
TACCGGCAGAGTGGCGTTTC+CGG 0.248184 1_2:+59173383 None:intergenic
TGTTGCTGCTGTTGTGTATT+GGG 0.274558 1_2:+59173118 None:intergenic
AGAGTGGCGTTTCCGGTCTA+AGG 0.283984 1_2:+59173390 None:intergenic
TTCTTTCTACGCTCACATTA+AGG 0.284952 1_2:-59173664 Msa0080790:intron
GGGACGTGGGTGTGGTGAGG+AGG 0.304968 1_2:-59173473 Msa0080790:five_prime_UTR
TCATTATAGCCGTAAAATAA+TGG 0.308576 1_2:+59172828 None:intergenic
ATAGATTTGTGCATTACCTT+TGG 0.321187 1_2:-59172867 Msa0080790:exon
TAGATTTGTGCATTACCTTT+GGG 0.342046 1_2:-59172866 Msa0080790:three_prime_UTR
GGAATAGAGTTCTGGTGTTC+GGG 0.348282 1_2:+59173316 None:intergenic
ATCTATCCCCAGTCGAGAAA+TGG 0.349618 1_2:+59172884 None:intergenic
ATGTGAGCGTAGAAAGAAAT+TGG 0.367973 1_2:+59173669 None:intergenic
TGGAATAGAGTTCTGGTGTT+CGG 0.377509 1_2:+59173315 None:intergenic
TTGTTGCTGCTGTTGTGTAT+TGG 0.380624 1_2:+59173117 None:intergenic
GACGTGGGTGTGGTGAGGAG+GGG 0.392828 1_2:-59173471 Msa0080790:five_prime_UTR
GGACGTGGGTGTGGTGAGGA+GGG 0.402639 1_2:-59173472 Msa0080790:five_prime_UTR
CAGGCAGAACGGCGGCGTCC+TGG 0.417159 1_2:+59173739 None:intergenic
ATGGTGGTGGAATAGAGTTC+TGG 0.430967 1_2:+59173308 None:intergenic
GGAATCGTACCAACGAGCTT+TGG 0.434321 1_2:+59173345 None:intergenic
GTAAAATAATGGATGCATAC+AGG 0.443850 1_2:+59172839 None:intergenic
GTTCTGGTGTTCGGGTTTGA+AGG 0.464430 1_2:+59173324 None:intergenic
AACGGAGGCGTCGCATAGAA+TGG 0.465306 1_2:+59173710 None:intergenic
ACGCAGAAGGGTTGATTAGA+TGG 0.466779 1_2:+59173289 None:intergenic
CAACCTCCCATTTCTCGACT+GGG 0.475626 1_2:-59172891 Msa0080790:CDS
TCTATCCCCAGTCGAGAAAT+GGG 0.477681 1_2:+59172885 None:intergenic
CCATATTTGTAGTTGGGACG+TGG 0.479603 1_2:-59173487 Msa0080790:intron
TTGTAGTTGGGACGTGGGTG+TGG 0.482685 1_2:-59173481 Msa0080790:intron
GGCTTGTTTGTGTTGAATAC+CGG 0.496651 1_2:+59173366 None:intergenic
CCAACCTCCCATTTCTCGAC+TGG 0.502817 1_2:-59172892 Msa0080790:CDS
GAGTATCCACCCTCAGGTGG+AGG 0.504269 1_2:+59172914 None:intergenic
GTCACTGCAGCCTCCACCTG+AGG 0.504311 1_2:-59172924 Msa0080790:CDS
TACTGGTTATTACAACTACT+AGG 0.507008 1_2:-59172759 Msa0080790:three_prime_UTR
GATACCATGGGAGTGTCATT+CGG 0.508317 1_2:-59172959 Msa0080790:CDS
TCAGGTGGAGGCTGCAGTGA+CGG 0.508961 1_2:+59172926 None:intergenic
GACCGGAAACGCCACTCTGC+CGG 0.512557 1_2:-59173385 Msa0080790:CDS
AGATATGTATAGAGGAGAGA+TGG 0.512762 1_2:+59172689 None:intergenic
AGGTTGGAGTATCCACCCTC+AGG 0.521427 1_2:+59172908 None:intergenic
AAGGGTTGATTAGATGGTGG+TGG 0.521880 1_2:+59173295 None:intergenic
TTCAAGATACACCCTTAGAC+CGG 0.529755 1_2:-59173402 Msa0080790:CDS
CCGGCGCGACACCGTTCTCC+AGG 0.530698 1_2:-59173757 Msa0080790:five_prime_UTR
CAGAGGTTGTCGAAGCAAAA+CGG 0.531061 1_2:+59173791 None:intergenic
AACATACTAATAGTTCTGAT+AGG 0.533532 1_2:-59172805 Msa0080790:three_prime_UTR
AACCTCTGTTCTAGTAGGTC+CGG 0.537233 1_2:-59173776 Msa0080790:five_prime_UTR
ATGTATGTAGGGAATATAGT+AGG 0.538369 1_2:-59172983 Msa0080790:intron
TCGACAACCTCTGTTCTAGT+AGG 0.555453 1_2:-59173781 Msa0080790:five_prime_UTR
GTTGGGACGTGGGTGTGGTG+AGG 0.558522 1_2:-59173476 Msa0080790:five_prime_UTR
AACGGCGGCGTCCTGGAGAA+CGG 0.558689 1_2:+59173746 None:intergenic
TCGCATAGAATGGAAGTGGC+AGG 0.559621 1_2:+59173720 None:intergenic
TCACTGCAGCCTCCACCTGA+GGG 0.562993 1_2:-59172923 Msa0080790:CDS
ATAGTACCGTGTTGACGAAG+TGG 0.572113 1_2:+59173266 None:intergenic
CCAGTCGAGAAATGGGAGGT+TGG 0.572331 1_2:+59172892 None:intergenic
CCTGGAGAACGGTGTCGCGC+CGG 0.577030 1_2:+59173757 None:intergenic
GGCGTCGCATAGAATGGAAG+TGG 0.581345 1_2:+59173716 None:intergenic
GAGTGGCGTTTCCGGTCTAA+GGG 0.584406 1_2:+59173391 None:intergenic
TACAGCGTCGTCGCGCAGAA+CGG 0.586040 1_2:+59173692 None:intergenic
AATGGAAGTGGCAGGCAGAA+CGG 0.586179 1_2:+59173728 None:intergenic
TAAAATAATGGATGCATACA+GGG 0.586745 1_2:+59172840 None:intergenic
GTCAGACTCAGACACAAGGC+AGG 0.587110 1_2:+59173819 None:intergenic
CAACCAATAACAAGAATGCC+AGG 0.589593 1_2:-59173097 Msa0080790:intron
TTGACGAAGTGGACGCAGAA+GGG 0.593699 1_2:+59173277 None:intergenic
GTTGACGAAGTGGACGCAGA+AGG 0.597332 1_2:+59173276 None:intergenic
TTGTCGAAGCAAAACGGAAG+GGG 0.600576 1_2:+59173797 None:intergenic
CTGCAGCCTCCACCTGAGGG+TGG 0.602600 1_2:-59172920 Msa0080790:CDS
CATATTTGTAGTTGGGACGT+GGG 0.611709 1_2:-59173486 Msa0080790:intron
CGCCGGACCTACTAGAACAG+AGG 0.614696 1_2:+59173774 None:intergenic
GAATATAGTAGGAGATACCA+TGG 0.619077 1_2:-59172972 Msa0080790:CDS
CTGCGTCCACTTCGTCAACA+CGG 0.619782 1_2:-59173272 Msa0080790:intron
ATGCATACAGGGAGGCCCAA+AGG 0.621250 1_2:+59172851 None:intergenic
GGTGGAGGCTGCAGTGACGG+AGG 0.622645 1_2:+59172929 None:intergenic
TTGGAGTATCCACCCTCAGG+TGG 0.623820 1_2:+59172911 None:intergenic
AGCGTCGTCGCGCAGAACGG+AGG 0.624495 1_2:+59173695 None:intergenic
GGTTGTCGAAGCAAAACGGA+AGG 0.626766 1_2:+59173795 None:intergenic
AAAGATCATAATTCATAACA+AGG 0.646758 1_2:+59172712 None:intergenic
ACAAACAAGCCAAAGCTCGT+TGG 0.650557 1_2:-59173354 Msa0080790:CDS
GTTGTCGAAGCAAAACGGAA+GGG 0.655524 1_2:+59173796 None:intergenic
CAGAAGGGTTGATTAGATGG+TGG 0.659317 1_2:+59173292 None:intergenic
AACCTCCCATTTCTCGACTG+GGG 0.663201 1_2:-59172890 Msa0080790:CDS
AGGGGTCAGACTCAGACACA+AGG 0.684771 1_2:+59173815 None:intergenic
GGAAGTGGCAGGCAGAACGG+CGG 0.688489 1_2:+59173731 None:intergenic
AATATAGTAGGAGATACCAT+GGG 0.697591 1_2:-59172971 Msa0080790:CDS
ATTGCCGAATGACACTCCCA+TGG 0.703596 1_2:+59172955 None:intergenic
TGTGTTGAATACCGGCAGAG+TGG 0.711189 1_2:+59173374 None:intergenic
ATCCCCAGTCGAGAAATGGG+AGG 0.723699 1_2:+59172888 None:intergenic
AATAATGGATGCATACAGGG+AGG 0.747327 1_2:+59172843 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATAGTTTCTTTCTTTA+TGG - chr1_2:59173443-59173462 Msa0080790:CDS 15.0%
!! ATTTATAGTTTCTTTCTTTA+TGG - chr1_2:59173443-59173462 Msa0080790:CDS 15.0%
!! CTAAGAAATGATAATTAATG+AGG + chr1_2:59173274-59173293 None:intergenic 20.0%
!! AAAGATCATAATTCATAACA+AGG + chr1_2:59173779-59173798 None:intergenic 20.0%
!!! TCTTTTTTAGATATGTATAG+AGG + chr1_2:59173810-59173829 None:intergenic 20.0%
!! CTAAGAAATGATAATTAATG+AGG + chr1_2:59173274-59173293 None:intergenic 20.0%
!! AAAGATCATAATTCATAACA+AGG + chr1_2:59173779-59173798 None:intergenic 20.0%
!!! TCTTTTTTAGATATGTATAG+AGG + chr1_2:59173810-59173829 None:intergenic 20.0%
! TTGAATCCATATTTGTAGTT+GGG - chr1_2:59172995-59173014 Msa0080790:intron 25.0%
!!! TCACAATAGTACTTTTTCAT+TGG + chr1_2:59173049-59173068 None:intergenic 25.0%
! TTAACTCTCTCTGTATATTT+AGG - chr1_2:59173309-59173328 Msa0080790:CDS 25.0%
! TAACTCTCTCTGTATATTTA+GGG - chr1_2:59173310-59173329 Msa0080790:CDS 25.0%
! ATCAGTAAATGAACAACTAT+CGG + chr1_2:59173425-59173444 None:intergenic 25.0%
!!! TAAAATAATGGATGCATACA+GGG + chr1_2:59173651-59173670 None:intergenic 25.0%
!!! TGTATGCATCCATTATTTTA+CGG - chr1_2:59173651-59173670 Msa0080790:intron 25.0%
! TCATTATAGCCGTAAAATAA+TGG + chr1_2:59173663-59173682 None:intergenic 25.0%
!!! AACATACTAATAGTTCTGAT+AGG - chr1_2:59173683-59173702 Msa0080790:five_prime_UTR 25.0%
! TAAAAAAGATAGCTACTGAT+TGG - chr1_2:59173821-59173840 Msa0080790:five_prime_UTR 25.0%
! TTGAATCCATATTTGTAGTT+GGG - chr1_2:59172995-59173014 Msa0080790:intron 25.0%
!!! TCACAATAGTACTTTTTCAT+TGG + chr1_2:59173049-59173068 None:intergenic 25.0%
! TTAACTCTCTCTGTATATTT+AGG - chr1_2:59173309-59173328 Msa0080790:CDS 25.0%
! TAACTCTCTCTGTATATTTA+GGG - chr1_2:59173310-59173329 Msa0080790:CDS 25.0%
! ATCAGTAAATGAACAACTAT+CGG + chr1_2:59173425-59173444 None:intergenic 25.0%
!!! TAAAATAATGGATGCATACA+GGG + chr1_2:59173651-59173670 None:intergenic 25.0%
!!! TGTATGCATCCATTATTTTA+CGG - chr1_2:59173651-59173670 Msa0080790:intron 25.0%
! TCATTATAGCCGTAAAATAA+TGG + chr1_2:59173663-59173682 None:intergenic 25.0%
!!! AACATACTAATAGTTCTGAT+AGG - chr1_2:59173683-59173702 Msa0080790:five_prime_UTR 25.0%
TCATACAAGTCGTTTGTAAT+TGG - chr1_2:59172853-59172872 Msa0080790:three_prime_UTR 30.0%
ATGAAACAAGAGCAAAGTTA+AGG + chr1_2:59172927-59172946 None:intergenic 30.0%
GTTGAATCCATATTTGTAGT+TGG - chr1_2:59172994-59173013 Msa0080790:intron 30.0%
! ATTTAGGGATTTTGTCGTTA+TGG - chr1_2:59173325-59173344 Msa0080790:CDS 30.0%
TCAGTAAATGAACAACTATC+GGG + chr1_2:59173424-59173443 None:intergenic 30.0%
TATGTACTATGCATGTATGT+AGG - chr1_2:59173493-59173512 Msa0080790:intron 30.0%
ATGTACTATGCATGTATGTA+GGG - chr1_2:59173494-59173513 Msa0080790:intron 30.0%
ATGTATGTAGGGAATATAGT+AGG - chr1_2:59173505-59173524 Msa0080790:intron 30.0%
AATATAGTAGGAGATACCAT+GGG - chr1_2:59173517-59173536 Msa0080790:intron 30.0%
ATAGATTTGTGCATTACCTT+TGG - chr1_2:59173621-59173640 Msa0080790:intron 30.0%
TAGATTTGTGCATTACCTTT+GGG - chr1_2:59173622-59173641 Msa0080790:intron 30.0%
!! GTAAAATAATGGATGCATAC+AGG + chr1_2:59173652-59173671 None:intergenic 30.0%
!!! CTGATTTTGAGTTTCTTTAC+TGG - chr1_2:59173712-59173731 Msa0080790:five_prime_UTR 30.0%
! TACTGGTTATTACAACTACT+AGG - chr1_2:59173729-59173748 Msa0080790:five_prime_UTR 30.0%
TCATACAAGTCGTTTGTAAT+TGG - chr1_2:59172853-59172872 Msa0080790:three_prime_UTR 30.0%
ATGAAACAAGAGCAAAGTTA+AGG + chr1_2:59172927-59172946 None:intergenic 30.0%
GTTGAATCCATATTTGTAGT+TGG - chr1_2:59172994-59173013 Msa0080790:intron 30.0%
! ATTTAGGGATTTTGTCGTTA+TGG - chr1_2:59173325-59173344 Msa0080790:CDS 30.0%
TCAGTAAATGAACAACTATC+GGG + chr1_2:59173424-59173443 None:intergenic 30.0%
TATGTACTATGCATGTATGT+AGG - chr1_2:59173493-59173512 Msa0080790:intron 30.0%
ATGTACTATGCATGTATGTA+GGG - chr1_2:59173494-59173513 Msa0080790:intron 30.0%
ATGTATGTAGGGAATATAGT+AGG - chr1_2:59173505-59173524 Msa0080790:intron 30.0%
AATATAGTAGGAGATACCAT+GGG - chr1_2:59173517-59173536 Msa0080790:intron 30.0%
ATAGATTTGTGCATTACCTT+TGG - chr1_2:59173621-59173640 Msa0080790:intron 30.0%
TAGATTTGTGCATTACCTTT+GGG - chr1_2:59173622-59173641 Msa0080790:intron 30.0%
!! GTAAAATAATGGATGCATAC+AGG + chr1_2:59173652-59173671 None:intergenic 30.0%
!!! CTGATTTTGAGTTTCTTTAC+TGG - chr1_2:59173712-59173731 Msa0080790:five_prime_UTR 30.0%
! TACTGGTTATTACAACTACT+AGG - chr1_2:59173729-59173748 Msa0080790:five_prime_UTR 30.0%
ATGTGAGCGTAGAAAGAAAT+TGG + chr1_2:59172822-59172841 None:intergenic 35.0%
TTCTTTCTACGCTCACATTA+AGG - chr1_2:59172824-59172843 Msa0080790:three_prime_UTR 35.0%
TACAAGTCGTTTGTAATTGG+CGG - chr1_2:59172856-59172875 Msa0080790:three_prime_UTR 35.0%
AGCAAAGTTAAGGATTGTTC+GGG + chr1_2:59172917-59172936 None:intergenic 35.0%
GAGCAAAGTTAAGGATTGTT+CGG + chr1_2:59172918-59172937 None:intergenic 35.0%
GAATATAGTAGGAGATACCA+TGG - chr1_2:59173516-59173535 Msa0080790:intron 35.0%
AGATATGTATAGAGGAGAGA+TGG + chr1_2:59173802-59173821 None:intergenic 35.0%
ATGTGAGCGTAGAAAGAAAT+TGG + chr1_2:59172822-59172841 None:intergenic 35.0%
TTCTTTCTACGCTCACATTA+AGG - chr1_2:59172824-59172843 Msa0080790:three_prime_UTR 35.0%
TACAAGTCGTTTGTAATTGG+CGG - chr1_2:59172856-59172875 Msa0080790:three_prime_UTR 35.0%
AGCAAAGTTAAGGATTGTTC+GGG + chr1_2:59172917-59172936 None:intergenic 35.0%
GAGCAAAGTTAAGGATTGTT+CGG + chr1_2:59172918-59172937 None:intergenic 35.0%
GAATATAGTAGGAGATACCA+TGG - chr1_2:59173516-59173535 Msa0080790:intron 35.0%
AGATATGTATAGAGGAGAGA+TGG + chr1_2:59173802-59173821 None:intergenic 35.0%
! CTCTTGTTTCATGCTCACAT+GGG - chr1_2:59172935-59172954 Msa0080790:CDS 40.0%
CATATTTGTAGTTGGGACGT+GGG - chr1_2:59173002-59173021 Msa0080790:intron 40.0%
TTCAAGATACACCCTTAGAC+CGG - chr1_2:59173086-59173105 Msa0080790:intron 40.0%
! GGCTTGTTTGTGTTGAATAC+CGG + chr1_2:59173125-59173144 None:intergenic 40.0%
! TGGAATAGAGTTCTGGTGTT+CGG + chr1_2:59173176-59173195 None:intergenic 40.0%
TGTTGCTGCTGTTGTGTATT+GGG + chr1_2:59173373-59173392 None:intergenic 40.0%
TTGTTGCTGCTGTTGTGTAT+TGG + chr1_2:59173374-59173393 None:intergenic 40.0%
CAACCAATAACAAGAATGCC+AGG - chr1_2:59173391-59173410 Msa0080790:CDS 40.0%
! TCACCTGGCATTCTTGTTAT+TGG + chr1_2:59173397-59173416 None:intergenic 40.0%
!! AATAATGGATGCATACAGGG+AGG + chr1_2:59173648-59173667 None:intergenic 40.0%
! CTCTTGTTTCATGCTCACAT+GGG - chr1_2:59172935-59172954 Msa0080790:CDS 40.0%
CATATTTGTAGTTGGGACGT+GGG - chr1_2:59173002-59173021 Msa0080790:intron 40.0%
TTCAAGATACACCCTTAGAC+CGG - chr1_2:59173086-59173105 Msa0080790:intron 40.0%
! GGCTTGTTTGTGTTGAATAC+CGG + chr1_2:59173125-59173144 None:intergenic 40.0%
! TGGAATAGAGTTCTGGTGTT+CGG + chr1_2:59173176-59173195 None:intergenic 40.0%
TGTTGCTGCTGTTGTGTATT+GGG + chr1_2:59173373-59173392 None:intergenic 40.0%
TTGTTGCTGCTGTTGTGTAT+TGG + chr1_2:59173374-59173393 None:intergenic 40.0%
CAACCAATAACAAGAATGCC+AGG - chr1_2:59173391-59173410 Msa0080790:CDS 40.0%
! TCACCTGGCATTCTTGTTAT+TGG + chr1_2:59173397-59173416 None:intergenic 40.0%
!! AATAATGGATGCATACAGGG+AGG + chr1_2:59173648-59173667 None:intergenic 40.0%
TTGTCGAAGCAAAACGGAAG+GGG + chr1_2:59172694-59172713 None:intergenic 45.0%
GTTGTCGAAGCAAAACGGAA+GGG + chr1_2:59172695-59172714 None:intergenic 45.0%
CAGAGGTTGTCGAAGCAAAA+CGG + chr1_2:59172700-59172719 None:intergenic 45.0%
TCGACAACCTCTGTTCTAGT+AGG - chr1_2:59172707-59172726 Msa0080790:three_prime_UTR 45.0%
AACCTCTGTTCTAGTAGGTC+CGG - chr1_2:59172712-59172731 Msa0080790:three_prime_UTR 45.0%
TTGCAACTTCAAGCTTCGTG+CGG + chr1_2:59172887-59172906 None:intergenic 45.0%
! GCTCTTGTTTCATGCTCACA+TGG - chr1_2:59172934-59172953 Msa0080790:CDS 45.0%
CCACGTCCCAACTACAAATA+TGG + chr1_2:59173004-59173023 None:intergenic 45.0%
CCATATTTGTAGTTGGGACG+TGG - chr1_2:59173001-59173020 Msa0080790:intron 45.0%
ACAAACAAGCCAAAGCTCGT+TGG - chr1_2:59173134-59173153 Msa0080790:CDS 45.0%
! GGAATAGAGTTCTGGTGTTC+GGG + chr1_2:59173175-59173194 None:intergenic 45.0%
!! ATGGTGGTGGAATAGAGTTC+TGG + chr1_2:59173183-59173202 None:intergenic 45.0%
! AAGGGTTGATTAGATGGTGG+TGG + chr1_2:59173196-59173215 None:intergenic 45.0%
! CAGAAGGGTTGATTAGATGG+TGG + chr1_2:59173199-59173218 None:intergenic 45.0%
! ACGCAGAAGGGTTGATTAGA+TGG + chr1_2:59173202-59173221 None:intergenic 45.0%
! ATAGTACCGTGTTGACGAAG+TGG + chr1_2:59173225-59173244 None:intergenic 45.0%
!! GATACCATGGGAGTGTCATT+CGG - chr1_2:59173529-59173548 Msa0080790:intron 45.0%
TCTATCCCCAGTCGAGAAAT+GGG + chr1_2:59173606-59173625 None:intergenic 45.0%
ATCTATCCCCAGTCGAGAAA+TGG + chr1_2:59173607-59173626 None:intergenic 45.0%
TTGTCGAAGCAAAACGGAAG+GGG + chr1_2:59172694-59172713 None:intergenic 45.0%
GTTGTCGAAGCAAAACGGAA+GGG + chr1_2:59172695-59172714 None:intergenic 45.0%
CAGAGGTTGTCGAAGCAAAA+CGG + chr1_2:59172700-59172719 None:intergenic 45.0%
TCGACAACCTCTGTTCTAGT+AGG - chr1_2:59172707-59172726 Msa0080790:three_prime_UTR 45.0%
AACCTCTGTTCTAGTAGGTC+CGG - chr1_2:59172712-59172731 Msa0080790:three_prime_UTR 45.0%
TTGCAACTTCAAGCTTCGTG+CGG + chr1_2:59172887-59172906 None:intergenic 45.0%
! GCTCTTGTTTCATGCTCACA+TGG - chr1_2:59172934-59172953 Msa0080790:CDS 45.0%
CCACGTCCCAACTACAAATA+TGG + chr1_2:59173004-59173023 None:intergenic 45.0%
CCATATTTGTAGTTGGGACG+TGG - chr1_2:59173001-59173020 Msa0080790:intron 45.0%
ACAAACAAGCCAAAGCTCGT+TGG - chr1_2:59173134-59173153 Msa0080790:CDS 45.0%
! GGAATAGAGTTCTGGTGTTC+GGG + chr1_2:59173175-59173194 None:intergenic 45.0%
!! ATGGTGGTGGAATAGAGTTC+TGG + chr1_2:59173183-59173202 None:intergenic 45.0%
! AAGGGTTGATTAGATGGTGG+TGG + chr1_2:59173196-59173215 None:intergenic 45.0%
! CAGAAGGGTTGATTAGATGG+TGG + chr1_2:59173199-59173218 None:intergenic 45.0%
! ACGCAGAAGGGTTGATTAGA+TGG + chr1_2:59173202-59173221 None:intergenic 45.0%
! ATAGTACCGTGTTGACGAAG+TGG + chr1_2:59173225-59173244 None:intergenic 45.0%
!! GATACCATGGGAGTGTCATT+CGG - chr1_2:59173529-59173548 Msa0080790:intron 45.0%
TCTATCCCCAGTCGAGAAAT+GGG + chr1_2:59173606-59173625 None:intergenic 45.0%
ATCTATCCCCAGTCGAGAAA+TGG + chr1_2:59173607-59173626 None:intergenic 45.0%
GGTTGTCGAAGCAAAACGGA+AGG + chr1_2:59172696-59172715 None:intergenic 50.0%
AATGGAAGTGGCAGGCAGAA+CGG + chr1_2:59172763-59172782 None:intergenic 50.0%
TCGCATAGAATGGAAGTGGC+AGG + chr1_2:59172771-59172790 None:intergenic 50.0%
TGTGTTGAATACCGGCAGAG+TGG + chr1_2:59173117-59173136 None:intergenic 50.0%
GGAATCGTACCAACGAGCTT+TGG + chr1_2:59173146-59173165 None:intergenic 50.0%
! GTTCTGGTGTTCGGGTTTGA+AGG + chr1_2:59173167-59173186 None:intergenic 50.0%
TTGACGAAGTGGACGCAGAA+GGG + chr1_2:59173214-59173233 None:intergenic 50.0%
ATTGCCGAATGACACTCCCA+TGG + chr1_2:59173536-59173555 None:intergenic 50.0%
CAACCTCCCATTTCTCGACT+GGG - chr1_2:59173597-59173616 Msa0080790:intron 50.0%
AACCTCCCATTTCTCGACTG+GGG - chr1_2:59173598-59173617 Msa0080790:intron 50.0%
GGTTGTCGAAGCAAAACGGA+AGG + chr1_2:59172696-59172715 None:intergenic 50.0%
AATGGAAGTGGCAGGCAGAA+CGG + chr1_2:59172763-59172782 None:intergenic 50.0%
TCGCATAGAATGGAAGTGGC+AGG + chr1_2:59172771-59172790 None:intergenic 50.0%
TGTGTTGAATACCGGCAGAG+TGG + chr1_2:59173117-59173136 None:intergenic 50.0%
GGAATCGTACCAACGAGCTT+TGG + chr1_2:59173146-59173165 None:intergenic 50.0%
! GTTCTGGTGTTCGGGTTTGA+AGG + chr1_2:59173167-59173186 None:intergenic 50.0%
TTGACGAAGTGGACGCAGAA+GGG + chr1_2:59173214-59173233 None:intergenic 50.0%
ATTGCCGAATGACACTCCCA+TGG + chr1_2:59173536-59173555 None:intergenic 50.0%
CAACCTCCCATTTCTCGACT+GGG - chr1_2:59173597-59173616 Msa0080790:intron 50.0%
AACCTCCCATTTCTCGACTG+GGG - chr1_2:59173598-59173617 Msa0080790:intron 50.0%
GTCAGACTCAGACACAAGGC+AGG + chr1_2:59172672-59172691 None:intergenic 55.0%
AGGGGTCAGACTCAGACACA+AGG + chr1_2:59172676-59172695 None:intergenic 55.0%
GGCGTCGCATAGAATGGAAG+TGG + chr1_2:59172775-59172794 None:intergenic 55.0%
!! AACGGAGGCGTCGCATAGAA+TGG + chr1_2:59172781-59172800 None:intergenic 55.0%
CAACTTCAAGCTTCGTGCGG+TGG + chr1_2:59172884-59172903 None:intergenic 55.0%
TTGTAGTTGGGACGTGGGTG+TGG - chr1_2:59173007-59173026 Msa0080790:intron 55.0%
GAGTGGCGTTTCCGGTCTAA+GGG + chr1_2:59173100-59173119 None:intergenic 55.0%
AGAGTGGCGTTTCCGGTCTA+AGG + chr1_2:59173101-59173120 None:intergenic 55.0%
GTTGACGAAGTGGACGCAGA+AGG + chr1_2:59173215-59173234 None:intergenic 55.0%
CTGCGTCCACTTCGTCAACA+CGG - chr1_2:59173216-59173235 Msa0080790:intron 55.0%
! TTGGAGTATCCACCCTCAGG+TGG + chr1_2:59173580-59173599 None:intergenic 55.0%
! AGGTTGGAGTATCCACCCTC+AGG + chr1_2:59173583-59173602 None:intergenic 55.0%
CCAGTCGAGAAATGGGAGGT+TGG + chr1_2:59173599-59173618 None:intergenic 55.0%
CCAACCTCCCATTTCTCGAC+TGG - chr1_2:59173596-59173615 Msa0080790:intron 55.0%
ATCCCCAGTCGAGAAATGGG+AGG + chr1_2:59173603-59173622 None:intergenic 55.0%
ATGCATACAGGGAGGCCCAA+AGG + chr1_2:59173640-59173659 None:intergenic 55.0%
GTCAGACTCAGACACAAGGC+AGG + chr1_2:59172672-59172691 None:intergenic 55.0%
AGGGGTCAGACTCAGACACA+AGG + chr1_2:59172676-59172695 None:intergenic 55.0%
GGCGTCGCATAGAATGGAAG+TGG + chr1_2:59172775-59172794 None:intergenic 55.0%
!! AACGGAGGCGTCGCATAGAA+TGG + chr1_2:59172781-59172800 None:intergenic 55.0%
CAACTTCAAGCTTCGTGCGG+TGG + chr1_2:59172884-59172903 None:intergenic 55.0%
TTGTAGTTGGGACGTGGGTG+TGG - chr1_2:59173007-59173026 Msa0080790:intron 55.0%
GAGTGGCGTTTCCGGTCTAA+GGG + chr1_2:59173100-59173119 None:intergenic 55.0%
AGAGTGGCGTTTCCGGTCTA+AGG + chr1_2:59173101-59173120 None:intergenic 55.0%
GTTGACGAAGTGGACGCAGA+AGG + chr1_2:59173215-59173234 None:intergenic 55.0%
CTGCGTCCACTTCGTCAACA+CGG - chr1_2:59173216-59173235 Msa0080790:intron 55.0%
! TTGGAGTATCCACCCTCAGG+TGG + chr1_2:59173580-59173599 None:intergenic 55.0%
! AGGTTGGAGTATCCACCCTC+AGG + chr1_2:59173583-59173602 None:intergenic 55.0%
CCAGTCGAGAAATGGGAGGT+TGG + chr1_2:59173599-59173618 None:intergenic 55.0%
CCAACCTCCCATTTCTCGAC+TGG - chr1_2:59173596-59173615 Msa0080790:intron 55.0%
ATCCCCAGTCGAGAAATGGG+AGG + chr1_2:59173603-59173622 None:intergenic 55.0%
ATGCATACAGGGAGGCCCAA+AGG + chr1_2:59173640-59173659 None:intergenic 55.0%
CGCCGGACCTACTAGAACAG+AGG + chr1_2:59172717-59172736 None:intergenic 60.0%
TACAGCGTCGTCGCGCAGAA+CGG + chr1_2:59172799-59172818 None:intergenic 60.0%
TACCGGCAGAGTGGCGTTTC+CGG + chr1_2:59173108-59173127 None:intergenic 60.0%
CAACTATCGGGTCGCTCACC+TGG + chr1_2:59173412-59173431 None:intergenic 60.0%
TCAGGTGGAGGCTGCAGTGA+CGG + chr1_2:59173565-59173584 None:intergenic 60.0%
TCACTGCAGCCTCCACCTGA+GGG - chr1_2:59173565-59173584 Msa0080790:intron 60.0%
GAGTATCCACCCTCAGGTGG+AGG + chr1_2:59173577-59173596 None:intergenic 60.0%
CGCCGGACCTACTAGAACAG+AGG + chr1_2:59172717-59172736 None:intergenic 60.0%
TACAGCGTCGTCGCGCAGAA+CGG + chr1_2:59172799-59172818 None:intergenic 60.0%
TACCGGCAGAGTGGCGTTTC+CGG + chr1_2:59173108-59173127 None:intergenic 60.0%
CAACTATCGGGTCGCTCACC+TGG + chr1_2:59173412-59173431 None:intergenic 60.0%
TCAGGTGGAGGCTGCAGTGA+CGG + chr1_2:59173565-59173584 None:intergenic 60.0%
TCACTGCAGCCTCCACCTGA+GGG - chr1_2:59173565-59173584 Msa0080790:intron 60.0%
GAGTATCCACCCTCAGGTGG+AGG + chr1_2:59173577-59173596 None:intergenic 60.0%
AACGGCGGCGTCCTGGAGAA+CGG + chr1_2:59172745-59172764 None:intergenic 65.0%
GGAAGTGGCAGGCAGAACGG+CGG + chr1_2:59172760-59172779 None:intergenic 65.0%
GTTGGGACGTGGGTGTGGTG+AGG - chr1_2:59173012-59173031 Msa0080790:intron 65.0%
GGACGTGGGTGTGGTGAGGA+GGG - chr1_2:59173016-59173035 Msa0080790:intron 65.0%
GACGTGGGTGTGGTGAGGAG+GGG - chr1_2:59173017-59173036 Msa0080790:intron 65.0%
GACCGGAAACGCCACTCTGC+CGG - chr1_2:59173103-59173122 Msa0080790:CDS 65.0%
GTCACTGCAGCCTCCACCTG+AGG - chr1_2:59173564-59173583 Msa0080790:intron 65.0%
AACGGCGGCGTCCTGGAGAA+CGG + chr1_2:59172745-59172764 None:intergenic 65.0%
GGAAGTGGCAGGCAGAACGG+CGG + chr1_2:59172760-59172779 None:intergenic 65.0%
GTTGGGACGTGGGTGTGGTG+AGG - chr1_2:59173012-59173031 Msa0080790:intron 65.0%
GGACGTGGGTGTGGTGAGGA+GGG - chr1_2:59173016-59173035 Msa0080790:intron 65.0%
GACGTGGGTGTGGTGAGGAG+GGG - chr1_2:59173017-59173036 Msa0080790:intron 65.0%
GACCGGAAACGCCACTCTGC+CGG - chr1_2:59173103-59173122 Msa0080790:CDS 65.0%
GTCACTGCAGCCTCCACCTG+AGG - chr1_2:59173564-59173583 Msa0080790:intron 65.0%
!! CCTGGAGAACGGTGTCGCGC+CGG + chr1_2:59172734-59172753 None:intergenic 70.0%
AGCGTCGTCGCGCAGAACGG+AGG + chr1_2:59172796-59172815 None:intergenic 70.0%
GGGACGTGGGTGTGGTGAGG+AGG - chr1_2:59173015-59173034 Msa0080790:intron 70.0%
GGTGGAGGCTGCAGTGACGG+AGG + chr1_2:59173562-59173581 None:intergenic 70.0%
CTGCAGCCTCCACCTGAGGG+TGG - chr1_2:59173568-59173587 Msa0080790:intron 70.0%
!! CCTGGAGAACGGTGTCGCGC+CGG + chr1_2:59172734-59172753 None:intergenic 70.0%
AGCGTCGTCGCGCAGAACGG+AGG + chr1_2:59172796-59172815 None:intergenic 70.0%
GGGACGTGGGTGTGGTGAGG+AGG - chr1_2:59173015-59173034 Msa0080790:intron 70.0%
GGTGGAGGCTGCAGTGACGG+AGG + chr1_2:59173562-59173581 None:intergenic 70.0%
CTGCAGCCTCCACCTGAGGG+TGG - chr1_2:59173568-59173587 Msa0080790:intron 70.0%
CCGGCGCGACACCGTTCTCC+AGG - chr1_2:59172731-59172750 Msa0080790:three_prime_UTR 75.0%
CAGGCAGAACGGCGGCGTCC+TGG + chr1_2:59172752-59172771 None:intergenic 75.0%
CCGGCGCGACACCGTTCTCC+AGG - chr1_2:59172731-59172750 Msa0080790:three_prime_UTR 75.0%
CAGGCAGAACGGCGGCGTCC+TGG + chr1_2:59172752-59172771 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 59172668 59173842 59172668 ID=Msa0080790;Name=Msa0080790
chr1_2 mRNA 59172668 59173842 59172668 ID=Msa0080790-mRNA-1;Parent=Msa0080790;Name=Msa0080790-mRNA-1;_AED=0.12;_eAED=0.12;_QI=208|0.66|0.75|1|1|1|4|218|128
chr1_2 exon 59172668 59172995 59172668 ID=Msa0080790-mRNA-1:exon:19142;Parent=Msa0080790-mRNA-1
chr1_2 exon 59173098 59173179 59173098 ID=Msa0080790-mRNA-1:exon:19141;Parent=Msa0080790-mRNA-1
chr1_2 exon 59173273 59173497 59173273 ID=Msa0080790-mRNA-1:exon:19140;Parent=Msa0080790-mRNA-1
chr1_2 exon 59173665 59173842 59173665 ID=Msa0080790-mRNA-1:exon:19139;Parent=Msa0080790-mRNA-1
chr1_2 five_prime_UTR 59173665 59173842 59173665 ID=Msa0080790-mRNA-1:five_prime_utr;Parent=Msa0080790-mRNA-1
chr1_2 five_prime_UTR 59173468 59173497 59173468 ID=Msa0080790-mRNA-1:five_prime_utr;Parent=Msa0080790-mRNA-1
chr1_2 CDS 59173273 59173467 59173273 ID=Msa0080790-mRNA-1:cds;Parent=Msa0080790-mRNA-1
chr1_2 CDS 59173098 59173179 59173098 ID=Msa0080790-mRNA-1:cds;Parent=Msa0080790-mRNA-1
chr1_2 CDS 59172886 59172995 59172886 ID=Msa0080790-mRNA-1:cds;Parent=Msa0080790-mRNA-1
chr1_2 three_prime_UTR 59172668 59172885 59172668 ID=Msa0080790-mRNA-1:three_prime_utr;Parent=Msa0080790-mRNA-1
Gene Sequence

>Msa0080790

ATGCCAATGAAAAAGTACTATTGTGAGTATTGCGATAAGCAATTTCAAGATACACCCTTAGACCGGAAACGCCACTCTGCCGGTATTCAACACAAACAAGCCAAAGCTCGTTGGTACGATTCCTTCAAACCCGAACACCAGAACTCTATTCCACCACCATCTAATCAACCCTTCTGCGTCCACTTCGTCAACACGGGATTTTGTCGTTATGGCGATTCTTGCAAATATTTTCATCCCAATACACAACAGCAGCAACAACCAATAACAAGAATGCCAGGGAATATAGTAGGAGATACCATGGGAGTGTCATTCGGCAATTTGCCTCCGTCACTGCAGCCTCCACCTGAGGGTGGATACTCCAACCTCCCATTTCTCGACTGGGGATAG
Protein sequence

>Msa0080790

MPMKKYYCEYCDKQFQDTPLDRKRHSAGIQHKQAKARWYDSFKPEHQNSIPPPSNQPFCVHFVNTGFCRYGDSCKYFHPNTQQQQQPITRMPGNIVGDTMGVSFGNLPPSLQPPPEGGYSNLPFLDWG*