AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0082460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082460 MtrunA17_Chr1g0185861 97.479 119 3 0 1 119 146 264 7.42e-82 245
Msa0082460 MtrunA17_Chr7g0259501 81.513 119 22 0 1 119 142 260 1.31e-68 211
Msa0082460 MtrunA17_Chr5g0440341 55.085 118 52 1 1 118 136 252 1.50e-36 128
Msa0082460 MtrunA17_Chr7g0254001 50.000 118 58 1 1 118 123 239 1.56e-32 117
Msa0082460 MtrunA17_Chr4g0002921 50.000 72 36 0 1 72 130 201 2.88e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082460 AT3G06190.3 69.492 118 36 0 1 118 137 254 2.58e-57 179
Msa0082460 AT3G06190.4 69.492 118 36 0 1 118 137 254 4.00e-57 179
Msa0082460 AT3G06190.5 68.908 119 37 0 1 119 137 255 6.65e-57 180
Msa0082460 AT3G06190.1 68.908 119 37 0 1 119 137 255 2.18e-56 180
Msa0082460 AT5G19000.1 67.227 119 39 0 1 119 138 256 3.35e-54 174
Msa0082460 AT5G19000.3 67.227 119 39 0 1 119 138 256 3.35e-54 174
Msa0082460 AT5G19000.2 67.227 119 39 0 1 119 138 256 6.33e-54 174
Msa0082460 AT3G06190.2 66.957 115 38 0 1 115 137 251 8.28e-51 162
Msa0082460 AT2G39760.4 55.085 118 53 0 1 118 129 246 4.70e-43 144
Msa0082460 AT2G39760.3 55.085 118 53 0 1 118 129 246 4.70e-43 144
Msa0082460 AT2G39760.2 55.085 118 53 0 1 118 129 246 6.01e-43 144
Msa0082460 AT2G39760.1 55.085 118 53 0 1 118 129 246 1.74e-42 144
Msa0082460 AT3G43700.3 53.390 118 54 1 1 118 140 256 4.63e-38 131
Msa0082460 AT3G43700.2 53.390 118 54 1 1 118 140 256 5.45e-38 131
Msa0082460 AT3G43700.1 53.390 118 54 1 1 118 140 256 1.15e-37 131
Msa0082460 AT3G03740.1 52.941 119 55 1 1 119 151 268 1.45e-35 127
Msa0082460 AT5G21010.1 51.695 118 56 1 1 118 133 249 5.93e-32 116

Find 97 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTAAGACAGTTATCTTTA+AGG 0.101359 1_2:-61310593 None:intergenic
TATGCTTAACAATTATAATA+AGG 0.211883 1_2:-61311039 None:intergenic
CATTGCTGCATGTTATATAT+TGG 0.221491 1_2:+61311760 Msa0082460:three_prime_UTR
TTATGCCTCCAAATGTTAAT+TGG 0.240517 1_2:+61311346 Msa0082460:three_prime_UTR
TGCCCAATCCTGCCTGCTTC+TGG 0.249468 1_2:+61311080 Msa0082460:three_prime_UTR
TTACTGGGATTAACTCAAAA+TGG 0.277584 1_2:+61311808 Msa0082460:three_prime_UTR
TCTACTATTGGTCACCAGTT+TGG 0.278284 1_2:+61310687 Msa0082460:CDS
TCACCAGTTTGGGAAGCTTT+TGG 0.292881 1_2:+61310698 Msa0082460:CDS
ATGCTTAACAATTATAATAA+GGG 0.315347 1_2:-61311038 None:intergenic
CCTGACATGCAAGAGCTTAC+TGG 0.322826 1_2:+61311792 Msa0082460:three_prime_UTR
CCAATATCACCTTCTACTAT+TGG 0.326476 1_2:+61310675 Msa0082460:CDS
CTACTATTGGTCACCAGTTT+GGG 0.329635 1_2:+61310688 Msa0082460:CDS
AACATGTTTGGTGGTGTTGC+AGG 0.335857 1_2:+61312805 Msa0082460:three_prime_UTR
TGCCTGCTTCTGGCATTAGT+TGG 0.341408 1_2:+61311090 Msa0082460:three_prime_UTR
AAACAGGAACCAAGCAATAA+AGG 0.342851 1_2:-61311519 None:intergenic
ATTGCTGCATGTTATATATT+GGG 0.354568 1_2:+61311761 Msa0082460:three_prime_UTR
ATTGGAATCGAAAATGTTCT+AGG 0.357925 1_2:-61310657 None:intergenic
CATCAGCCTAAGTCTATCTA+AGG 0.367304 1_2:-61311880 None:intergenic
CTTGATGGCAGTGATGTAAA+TGG 0.372844 1_2:+61312608 Msa0082460:three_prime_UTR
ATTATTTAAACCTTGAAAAC+AGG 0.377554 1_2:-61310882 None:intergenic
CCAATAGTAGAAGGTGATAT+TGG 0.380995 1_2:-61310675 None:intergenic
CCAGTAAGCTCTTGCATGTC+AGG 0.381423 1_2:-61311792 None:intergenic
GCCTGCTTCTGGCATTAGTT+GGG 0.382234 1_2:+61311091 Msa0082460:three_prime_UTR
ATATCAGGACAGAGACAAAA+AGG 0.383672 1_2:-61310981 None:intergenic
TTTCCAAAAGCTTCCCAAAC+TGG 0.384496 1_2:-61310701 None:intergenic
TCTTTATACCAATTAACATT+TGG 0.387856 1_2:-61311354 None:intergenic
ATGCCAGAAGCAGGCAGGAT+TGG 0.388898 1_2:-61311083 None:intergenic
ACTTGTGTTTAATGTGTTTG+TGG 0.388986 1_2:+61311642 Msa0082460:three_prime_UTR
CGAAGTTCAATATCTATATC+AGG 0.390971 1_2:-61310996 None:intergenic
GTTCACTGAGATGTGAGATT+TGG 0.399746 1_2:+61311401 Msa0082460:three_prime_UTR
TGCCAGAAGCAGGCAGGATT+GGG 0.403871 1_2:-61311082 None:intergenic
GTGAGTGGAACAGGGTTCTC+TGG 0.404158 1_2:-61312676 None:intergenic
GAAGTTTAATAACATGCATA+TGG 0.409642 1_2:+61311684 Msa0082460:three_prime_UTR
CGTGGCTACAACTTTAGCTT+TGG 0.416152 1_2:+61311941 Msa0082460:three_prime_UTR
GTTTGTTGAGTTGTTCTAGC+TGG 0.432322 1_2:+61311538 Msa0082460:three_prime_UTR
GGCTAGATTTACTGAGCATT+CGG 0.434481 1_2:+61312553 Msa0082460:three_prime_UTR
TCTAATTCTTATTAAACCAT+TGG 0.435416 1_2:+61311565 Msa0082460:three_prime_UTR
TCAAACAGACTGCTTTCAGC+TGG 0.445729 1_2:-61311983 None:intergenic
GGGAGAAGATCAACATTCAT+AGG 0.447618 1_2:+61312729 Msa0082460:three_prime_UTR
AAAATGGGCAACTACCTTGA+TGG 0.450276 1_2:+61311824 Msa0082460:three_prime_UTR
CTGACATGCAAGAGCTTACT+GGG 0.457209 1_2:+61311793 Msa0082460:three_prime_UTR
TGATGGTTTCGAGTACTTGA+AGG 0.459553 1_2:+61312490 Msa0082460:three_prime_UTR
ATATACTCGCAAATTGCAAC+TGG 0.462539 1_2:-61311197 None:intergenic
TGTGTGTTCTGATCTTTCAT+AGG 0.465282 1_2:-61310831 None:intergenic
TTACAGTGAACCGTGGATAG+AGG 0.466244 1_2:+61312708 Msa0082460:three_prime_UTR
TTATACCAATTAACATTTGG+AGG 0.472496 1_2:-61311351 None:intergenic
GGCAGGATTGGGCACTAAGT+TGG 0.477139 1_2:-61311071 None:intergenic
AGATGTAAATGTAACATGTT+TGG 0.478541 1_2:+61312793 Msa0082460:three_prime_UTR
AACTGGTGACCAATAGTAGA+AGG 0.481007 1_2:-61310684 None:intergenic
GCTAGAACAACTCAACAAAC+AGG 0.489414 1_2:-61311535 None:intergenic
CAATTTATACAAGAACTTGT+GGG 0.497315 1_2:-61311457 None:intergenic
ATGTTAATTGGTATAAAGAC+TGG 0.503415 1_2:+61311358 Msa0082460:three_prime_UTR
TCAATTTATACAAGAACTTG+TGG 0.506208 1_2:-61311458 None:intergenic
CTTCGTTCCTATGCTTGCAC+AGG 0.511010 1_2:-61312580 None:intergenic
TTGCGAGTATATTTGACTGT+TGG 0.512484 1_2:+61311208 Msa0082460:three_prime_UTR
ATCTGCTAGCAGCAGCTGAT+CGG 0.518356 1_2:+61311853 Msa0082460:three_prime_UTR
TACTGGGATTAACTCAAAAT+GGG 0.520060 1_2:+61311809 Msa0082460:three_prime_UTR
AACATTTGGAGGCATAAATG+AGG 0.520710 1_2:-61311340 None:intergenic
TCTGTTTGAAATTCATTGCA+AGG 0.524584 1_2:+61311997 Msa0082460:three_prime_UTR
CTCGCAAATTGCAACTGGCA+AGG 0.525911 1_2:-61311192 None:intergenic
AAAAGCTCAGTCAAAACAGA+TGG 0.526627 1_2:-61312521 None:intergenic
AACAGGGTTCTCTGGAGACC+TGG 0.530076 1_2:-61312668 None:intergenic
AACTAATGCCAGAAGCAGGC+AGG 0.538691 1_2:-61311088 None:intergenic
CTTAGTGTTAATTGTAGCGT+TGG 0.538942 1_2:+61310609 Msa0082460:CDS
TACAGTGAACCGTGGATAGA+GGG 0.541214 1_2:+61312709 Msa0082460:three_prime_UTR
GTTGCAGGTTTGTCTTCGAT+AGG 0.543065 1_2:+61312820 Msa0082460:three_prime_UTR
AGGAACGAAGCAATACTTGA+TGG 0.545226 1_2:+61312593 Msa0082460:three_prime_UTR
CAAATTGCAACTGGCAAGGG+TGG 0.549732 1_2:-61311188 None:intergenic
GAGGCATAAATGAGGGAAGC+GGG 0.556076 1_2:-61311332 None:intergenic
ACCCAACTAATGCCAGAAGC+AGG 0.556195 1_2:-61311092 None:intergenic
CTGCTTTCAGCTGGAAACAA+TGG 0.557661 1_2:-61311974 None:intergenic
TCGCAAATTGCAACTGGCAA+GGG 0.558231 1_2:-61311191 None:intergenic
TAGCAGATGCTGAGCCATCA+AGG 0.560135 1_2:-61311838 None:intergenic
CGTAAACTGCAGAAAGTCAA+AGG 0.563740 1_2:-61311600 None:intergenic
TGCTTAACAATTATAATAAG+GGG 0.564985 1_2:-61311037 None:intergenic
TTGGAATCGAAAATGTTCTA+GGG 0.573539 1_2:-61310656 None:intergenic
TAAAGGAAATAAAATCAGAA+AGG 0.573800 1_2:-61311502 None:intergenic
CGGACTTCCTGTGCAAGCAT+AGG 0.582301 1_2:+61312573 Msa0082460:three_prime_UTR
TGATCTTCTCCCTCTATCCA+CGG 0.587590 1_2:-61312718 None:intergenic
GGAGGCATAAATGAGGGAAG+CGG 0.592200 1_2:-61311333 None:intergenic
ACATTTGGAGGCATAAATGA+GGG 0.597916 1_2:-61311339 None:intergenic
CAAAGCTAAAGTTGTAGCCA+CGG 0.605118 1_2:-61311940 None:intergenic
TCAGCTGTGATGCAAACTGA+TGG 0.605667 1_2:+61312473 Msa0082460:intron
AATTTATACAAGAACTTGTG+GGG 0.608211 1_2:-61311456 None:intergenic
ATTGTAGCGTTGGTGTTGTG+AGG 0.611297 1_2:+61310619 Msa0082460:CDS
TGTAAATGTAACATGTTTGG+TGG 0.611863 1_2:+61312796 Msa0082460:three_prime_UTR
CAGTGATGTAAATGGAAGGC+GGG 0.613054 1_2:+61312616 Msa0082460:three_prime_UTR
AAAGCTCAGTCAAAACAGAT+GGG 0.629546 1_2:-61312520 None:intergenic
TATAAAAGTTGAAGATATCG+AGG 0.632506 1_2:+61310857 Msa0082460:CDS
GTTGTGAGGTCACACACAGA+GGG 0.645126 1_2:+61310633 Msa0082460:CDS
GGTATGCCTTAGATAGACTT+AGG 0.647862 1_2:+61311874 Msa0082460:three_prime_UTR
ATGGCAGTGATGTAAATGGA+AGG 0.702793 1_2:+61312612 Msa0082460:three_prime_UTR
AGATGTTGCAATAAATACCG+TGG 0.705274 1_2:+61311923 Msa0082460:three_prime_UTR
GCAGTGATGTAAATGGAAGG+CGG 0.707656 1_2:+61312615 Msa0082460:three_prime_UTR
TGTTGTGAGGTCACACACAG+AGG 0.714300 1_2:+61310632 Msa0082460:CDS
GCAAGGCCTGAAAATCTCAG+AGG 0.724609 1_2:+61312014 Msa0082460:three_prime_UTR
AAACTGCAGAAAGTCAAAGG+CGG 0.764442 1_2:-61311597 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCTTAACAATTATAATAA+GGG - chr1_2:61311041-61311060 None:intergenic 15.0%
!! TATGCTTAACAATTATAATA+AGG - chr1_2:61311042-61311061 None:intergenic 15.0%
!!! AATGTTGATAAAAAAAATAG+AGG - chr1_2:61311305-61311324 None:intergenic 15.0%
!! TCAAAATAGAGAAAAAAATA+TGG - chr1_2:61311737-61311756 None:intergenic 15.0%
!!! TATTTTTTTCTCTATTTTGA+AGG + chr1_2:61311737-61311756 Msa0082460:three_prime_UTR 15.0%
!!! ATTGTTTTAAATTGTGTTTC+AGG + chr1_2:61310523-61310542 Msa0082460:intron 20.0%
!!! TTGTTTTAAATTGTGTTTCA+GGG + chr1_2:61310524-61310543 Msa0082460:intron 20.0%
!! ATTATTTAAACCTTGAAAAC+AGG - chr1_2:61310885-61310904 None:intergenic 20.0%
!! TGCTTAACAATTATAATAAG+GGG - chr1_2:61311040-61311059 None:intergenic 20.0%
!! AGAACACAACAATAAAAATA+TGG + chr1_2:61311262-61311281 Msa0082460:three_prime_UTR 20.0%
!! TCTTTATACCAATTAACATT+TGG - chr1_2:61311357-61311376 None:intergenic 20.0%
!! TAAAGGAAATAAAATCAGAA+AGG - chr1_2:61311505-61311524 None:intergenic 20.0%
!!! ATTTTATTTCCTTTATTGCT+TGG + chr1_2:61311510-61311529 Msa0082460:three_prime_UTR 20.0%
!! TCTAATTCTTATTAAACCAT+TGG + chr1_2:61311565-61311584 Msa0082460:three_prime_UTR 20.0%
!! AGAAAAATATGTGCAATATT+AGG + chr1_2:61312086-61312105 Msa0082460:intron 20.0%
!! GAAAAATATGTGCAATATTA+GGG + chr1_2:61312087-61312106 Msa0082460:intron 20.0%
!! AAAAATATGTGCAATATTAG+GGG + chr1_2:61312088-61312107 Msa0082460:intron 20.0%
!!! GTTCTTAAACTCTTTTAATT+AGG + chr1_2:61312303-61312322 Msa0082460:intron 20.0%
! AATCAACAAATGCATTCAAT+GGG - chr1_2:61310419-61310438 None:intergenic 25.0%
! AAATCAACAAATGCATTCAA+TGG - chr1_2:61310420-61310439 None:intergenic 25.0%
!!! TTGAATGCATTTGTTGATTT+TGG + chr1_2:61310420-61310439 Msa0082460:intron 25.0%
!!! TGTTTTAAATTGTGTTTCAG+GGG + chr1_2:61310525-61310544 Msa0082460:intron 25.0%
! TATAAAAGTTGAAGATATCG+AGG + chr1_2:61310857-61310876 Msa0082460:CDS 25.0%
! ACATGTTTGCAAAAAAACAT+TGG + chr1_2:61311128-61311147 Msa0082460:three_prime_UTR 25.0%
! TTATACCAATTAACATTTGG+AGG - chr1_2:61311354-61311373 None:intergenic 25.0%
! ATGTTAATTGGTATAAAGAC+TGG + chr1_2:61311358-61311377 Msa0082460:three_prime_UTR 25.0%
! AATTTATACAAGAACTTGTG+GGG - chr1_2:61311459-61311478 None:intergenic 25.0%
! CAATTTATACAAGAACTTGT+GGG - chr1_2:61311460-61311479 None:intergenic 25.0%
! TCAATTTATACAAGAACTTG+TGG - chr1_2:61311461-61311480 None:intergenic 25.0%
! GAAGTTTAATAACATGCATA+TGG + chr1_2:61311684-61311703 Msa0082460:three_prime_UTR 25.0%
! ATTGCTGCATGTTATATATT+GGG + chr1_2:61311761-61311780 Msa0082460:three_prime_UTR 25.0%
!! AATACTTCAGTTGATATCAT+AGG - chr1_2:61312435-61312454 None:intergenic 25.0%
! AGATGTAAATGTAACATGTT+TGG + chr1_2:61312793-61312812 Msa0082460:three_prime_UTR 25.0%
!!! TGCATTTGTTGATTTTGGTT+TGG + chr1_2:61310425-61310444 Msa0082460:intron 30.0%
!!! ATGGAACTTTTTTGTGACAA+TGG + chr1_2:61310475-61310494 Msa0082460:intron 30.0%
CACTAAGACAGTTATCTTTA+AGG - chr1_2:61310596-61310615 None:intergenic 30.0%
TTGGAATCGAAAATGTTCTA+GGG - chr1_2:61310659-61310678 None:intergenic 30.0%
ATTGGAATCGAAAATGTTCT+AGG - chr1_2:61310660-61310679 None:intergenic 30.0%
!!! GATGTGAATTTTGAAGTGAA+TGG + chr1_2:61310738-61310757 Msa0082460:CDS 30.0%
! AGTGAATGGTGAAATTTTTG+CGG + chr1_2:61310752-61310771 Msa0082460:CDS 30.0%
! AATTTTTGCGGCTCATAAAT+TGG + chr1_2:61310764-61310783 Msa0082460:CDS 30.0%
CGAAGTTCAATATCTATATC+AGG - chr1_2:61310999-61311018 None:intergenic 30.0%
TTATGCCTCCAAATGTTAAT+TGG + chr1_2:61311346-61311365 Msa0082460:three_prime_UTR 30.0%
!!! ACTGGTATTTTTTCAGATTG+AGG + chr1_2:61311376-61311395 Msa0082460:three_prime_UTR 30.0%
ACTTGTGTTTAATGTGTTTG+TGG + chr1_2:61311642-61311661 Msa0082460:three_prime_UTR 30.0%
CATTGCTGCATGTTATATAT+TGG + chr1_2:61311760-61311779 Msa0082460:three_prime_UTR 30.0%
TTACTGGGATTAACTCAAAA+TGG + chr1_2:61311808-61311827 Msa0082460:three_prime_UTR 30.0%
TACTGGGATTAACTCAAAAT+GGG + chr1_2:61311809-61311828 Msa0082460:three_prime_UTR 30.0%
! TCTGTTTGAAATTCATTGCA+AGG + chr1_2:61311997-61312016 Msa0082460:three_prime_UTR 30.0%
! CGTATGCTTATGCTTTATTA+AGG + chr1_2:61312200-61312219 Msa0082460:intron 30.0%
AAATCAAGCTTAGTGACTTT+GGG + chr1_2:61312261-61312280 Msa0082460:intron 30.0%
AATTAGGATGCTGTATATGA+TGG + chr1_2:61312319-61312338 Msa0082460:intron 30.0%
TACAAATTTACCAACGCTTT+GGG - chr1_2:61312359-61312378 None:intergenic 30.0%
ATACAAATTTACCAACGCTT+TGG - chr1_2:61312360-61312379 None:intergenic 30.0%
TCACAGCTGAAAAAAAGAAA+GGG - chr1_2:61312463-61312482 None:intergenic 30.0%
ATCACAGCTGAAAAAAAGAA+AGG - chr1_2:61312464-61312483 None:intergenic 30.0%
! CTGTAATTTTTGAATGTGAG+TGG - chr1_2:61312694-61312713 None:intergenic 30.0%
TGTAAATGTAACATGTTTGG+TGG + chr1_2:61312796-61312815 Msa0082460:three_prime_UTR 30.0%
!!! CTTTTTTGTGACAATGGAAG+AGG + chr1_2:61310481-61310500 Msa0082460:intron 35.0%
!! GAAGAGGTACAGTTTTCTAA+AGG + chr1_2:61310497-61310516 Msa0082460:intron 35.0%
CTTAGTGTTAATTGTAGCGT+TGG + chr1_2:61310609-61310628 Msa0082460:CDS 35.0%
CCAATAGTAGAAGGTGATAT+TGG - chr1_2:61310678-61310697 None:intergenic 35.0%
CCAATATCACCTTCTACTAT+TGG + chr1_2:61310675-61310694 Msa0082460:CDS 35.0%
!! AAAAGCTGAGCTCTAAAAAC+AGG - chr1_2:61310807-61310826 None:intergenic 35.0%
!!! GTTTTTAGAGCTCAGCTTTT+TGG + chr1_2:61310807-61310826 Msa0082460:CDS 35.0%
TGTGTGTTCTGATCTTTCAT+AGG - chr1_2:61310834-61310853 None:intergenic 35.0%
ATATCAGGACAGAGACAAAA+AGG - chr1_2:61310984-61311003 None:intergenic 35.0%
ATATACTCGCAAATTGCAAC+TGG - chr1_2:61311200-61311219 None:intergenic 35.0%
TTGCGAGTATATTTGACTGT+TGG + chr1_2:61311208-61311227 Msa0082460:three_prime_UTR 35.0%
! ACATTTGGAGGCATAAATGA+GGG - chr1_2:61311342-61311361 None:intergenic 35.0%
! AACATTTGGAGGCATAAATG+AGG - chr1_2:61311343-61311362 None:intergenic 35.0%
AAACAGGAACCAAGCAATAA+AGG - chr1_2:61311522-61311541 None:intergenic 35.0%
AGATGTTGCAATAAATACCG+TGG + chr1_2:61311923-61311942 Msa0082460:three_prime_UTR 35.0%
ATGTGCAATATTAGGGGAAT+TGG + chr1_2:61312094-61312113 Msa0082460:intron 35.0%
TGTGCAATATTAGGGGAATT+GGG + chr1_2:61312095-61312114 Msa0082460:intron 35.0%
ATAGCAACATACAGACTTTC+AGG - chr1_2:61312132-61312151 None:intergenic 35.0%
CAAATCAAGCTTAGTGACTT+TGG + chr1_2:61312260-61312279 Msa0082460:intron 35.0%
GGTCTGGATGAAGAATAAAT+AGG - chr1_2:61312414-61312433 None:intergenic 35.0%
! TTCAGTTGATATCATAGGTC+TGG - chr1_2:61312430-61312449 None:intergenic 35.0%
AAAGCTCAGTCAAAACAGAT+GGG - chr1_2:61312523-61312542 None:intergenic 35.0%
AAAAGCTCAGTCAAAACAGA+TGG - chr1_2:61312524-61312543 None:intergenic 35.0%
!!! ATCTGTTTTGACTGAGCTTT+TGG + chr1_2:61312523-61312542 Msa0082460:three_prime_UTR 35.0%
CTATACTGTGAAAAATGACC+AGG + chr1_2:61312650-61312669 Msa0082460:three_prime_UTR 35.0%
! TTTTGAATGTGAGTGGAACA+GGG - chr1_2:61312687-61312706 None:intergenic 35.0%
! TTTTTGAATGTGAGTGGAAC+AGG - chr1_2:61312688-61312707 None:intergenic 35.0%
TCAAAAATTACAGTGAACCG+TGG + chr1_2:61312701-61312720 Msa0082460:three_prime_UTR 35.0%
TTCCTGCTGAATTGAGTTCA+TGG + chr1_2:61310456-61310475 Msa0082460:intron 40.0%
TTCCATGAACTCAATTCAGC+AGG - chr1_2:61310461-61310480 None:intergenic 40.0%
AACTGGTGACCAATAGTAGA+AGG - chr1_2:61310687-61310706 None:intergenic 40.0%
TCTACTATTGGTCACCAGTT+TGG + chr1_2:61310687-61310706 Msa0082460:CDS 40.0%
CTACTATTGGTCACCAGTTT+GGG + chr1_2:61310688-61310707 Msa0082460:CDS 40.0%
TTTCCAAAAGCTTCCCAAAC+TGG - chr1_2:61310704-61310723 None:intergenic 40.0%
!! TTTGGGAAGCTTTTGGAAAG+TGG + chr1_2:61310705-61310724 Msa0082460:CDS 40.0%
GTTCACTGAGATGTGAGATT+TGG + chr1_2:61311401-61311420 Msa0082460:three_prime_UTR 40.0%
GCTAGAACAACTCAACAAAC+AGG - chr1_2:61311538-61311557 None:intergenic 40.0%
! GTTTGTTGAGTTGTTCTAGC+TGG + chr1_2:61311538-61311557 Msa0082460:three_prime_UTR 40.0%
AAGGCGGTTTGAAAAACCAA+TGG - chr1_2:61311584-61311603 None:intergenic 40.0%
AAACTGCAGAAAGTCAAAGG+CGG - chr1_2:61311600-61311619 None:intergenic 40.0%
CGTAAACTGCAGAAAGTCAA+AGG - chr1_2:61311603-61311622 None:intergenic 40.0%
AAAATGGGCAACTACCTTGA+TGG + chr1_2:61311824-61311843 Msa0082460:three_prime_UTR 40.0%
GGTATGCCTTAGATAGACTT+AGG + chr1_2:61311874-61311893 Msa0082460:three_prime_UTR 40.0%
CATCAGCCTAAGTCTATCTA+AGG - chr1_2:61311883-61311902 None:intergenic 40.0%
CAAAGCTAAAGTTGTAGCCA+CGG - chr1_2:61311943-61311962 None:intergenic 40.0%
!!! AACACACCTCTGAGATTTTC+AGG - chr1_2:61312023-61312042 None:intergenic 40.0%
AGGGGAATTGGGACATTAAA+AGG + chr1_2:61312106-61312125 Msa0082460:intron 40.0%
AGCATACGATAGTCAACAAC+AGG - chr1_2:61312188-61312207 None:intergenic 40.0%
! TACAGTTTTACCCAAAGCGT+TGG + chr1_2:61312346-61312365 Msa0082460:intron 40.0%
! TGATGGTTTCGAGTACTTGA+AGG + chr1_2:61312490-61312509 Msa0082460:three_prime_UTR 40.0%
GGCTAGATTTACTGAGCATT+CGG + chr1_2:61312553-61312572 Msa0082460:three_prime_UTR 40.0%
AGGAACGAAGCAATACTTGA+TGG + chr1_2:61312593-61312612 Msa0082460:three_prime_UTR 40.0%
! CTTGATGGCAGTGATGTAAA+TGG + chr1_2:61312608-61312627 Msa0082460:three_prime_UTR 40.0%
ATGGCAGTGATGTAAATGGA+AGG + chr1_2:61312612-61312631 Msa0082460:three_prime_UTR 40.0%
GGGAGAAGATCAACATTCAT+AGG + chr1_2:61312729-61312748 Msa0082460:three_prime_UTR 40.0%
!! ATTGTAGCGTTGGTGTTGTG+AGG + chr1_2:61310619-61310638 Msa0082460:CDS 45.0%
! TCACCAGTTTGGGAAGCTTT+TGG + chr1_2:61310698-61310717 Msa0082460:CDS 45.0%
! TATCGAGGCTCCTGTTTTCA+AGG + chr1_2:61310872-61310891 Msa0082460:CDS 45.0%
TCGCAAATTGCAACTGGCAA+GGG - chr1_2:61311194-61311213 None:intergenic 45.0%
CTGACATGCAAGAGCTTACT+GGG + chr1_2:61311793-61311812 Msa0082460:three_prime_UTR 45.0%
! CGTGGCTACAACTTTAGCTT+TGG + chr1_2:61311941-61311960 Msa0082460:three_prime_UTR 45.0%
CTGCTTTCAGCTGGAAACAA+TGG - chr1_2:61311977-61311996 None:intergenic 45.0%
TCAAACAGACTGCTTTCAGC+TGG - chr1_2:61311986-61312005 None:intergenic 45.0%
TCAGCTGTGATGCAAACTGA+TGG + chr1_2:61312473-61312492 Msa0082460:intron 45.0%
!!! GACTGAGCTTTTGGAGTATG+TGG + chr1_2:61312532-61312551 Msa0082460:three_prime_UTR 45.0%
GCAGTGATGTAAATGGAAGG+CGG + chr1_2:61312615-61312634 Msa0082460:three_prime_UTR 45.0%
CAGTGATGTAAATGGAAGGC+GGG + chr1_2:61312616-61312635 Msa0082460:three_prime_UTR 45.0%
TTACAGTGAACCGTGGATAG+AGG + chr1_2:61312708-61312727 Msa0082460:three_prime_UTR 45.0%
TACAGTGAACCGTGGATAGA+GGG + chr1_2:61312709-61312728 Msa0082460:three_prime_UTR 45.0%
TGATCTTCTCCCTCTATCCA+CGG - chr1_2:61312721-61312740 None:intergenic 45.0%
!! AACATGTTTGGTGGTGTTGC+AGG + chr1_2:61312805-61312824 Msa0082460:three_prime_UTR 45.0%
GTTGCAGGTTTGTCTTCGAT+AGG + chr1_2:61312820-61312839 Msa0082460:three_prime_UTR 45.0%
AGCAAGCAAAAAAGGGACAG+AGG - chr1_2:61312916-61312935 None:intergenic 45.0%
TGTTGTGAGGTCACACACAG+AGG + chr1_2:61310632-61310651 Msa0082460:CDS 50.0%
GTTGTGAGGTCACACACAGA+GGG + chr1_2:61310633-61310652 Msa0082460:CDS 50.0%
AACTAATGCCAGAAGCAGGC+AGG - chr1_2:61311091-61311110 None:intergenic 50.0%
TGCCTGCTTCTGGCATTAGT+TGG + chr1_2:61311090-61311109 Msa0082460:three_prime_UTR 50.0%
GCCTGCTTCTGGCATTAGTT+GGG + chr1_2:61311091-61311110 Msa0082460:three_prime_UTR 50.0%
ACCCAACTAATGCCAGAAGC+AGG - chr1_2:61311095-61311114 None:intergenic 50.0%
CAAATTGCAACTGGCAAGGG+TGG - chr1_2:61311191-61311210 None:intergenic 50.0%
CTCGCAAATTGCAACTGGCA+AGG - chr1_2:61311195-61311214 None:intergenic 50.0%
GAGGCATAAATGAGGGAAGC+GGG - chr1_2:61311335-61311354 None:intergenic 50.0%
! GGAGGCATAAATGAGGGAAG+CGG - chr1_2:61311336-61311355 None:intergenic 50.0%
CCAGTAAGCTCTTGCATGTC+AGG - chr1_2:61311795-61311814 None:intergenic 50.0%
CCTGACATGCAAGAGCTTAC+TGG + chr1_2:61311792-61311811 Msa0082460:three_prime_UTR 50.0%
TAGCAGATGCTGAGCCATCA+AGG - chr1_2:61311841-61311860 None:intergenic 50.0%
ATCTGCTAGCAGCAGCTGAT+CGG + chr1_2:61311853-61311872 Msa0082460:three_prime_UTR 50.0%
GCAAGGCCTGAAAATCTCAG+AGG + chr1_2:61312014-61312033 Msa0082460:three_prime_UTR 50.0%
CTTCGTTCCTATGCTTGCAC+AGG - chr1_2:61312583-61312602 None:intergenic 50.0%
GGCAGGATTGGGCACTAAGT+TGG - chr1_2:61311074-61311093 None:intergenic 55.0%
TGCCAGAAGCAGGCAGGATT+GGG - chr1_2:61311085-61311104 None:intergenic 55.0%
ATGCCAGAAGCAGGCAGGAT+TGG - chr1_2:61311086-61311105 None:intergenic 55.0%
CGGACTTCCTGTGCAAGCAT+AGG + chr1_2:61312573-61312592 Msa0082460:three_prime_UTR 55.0%
! AACAGGGTTCTCTGGAGACC+TGG - chr1_2:61312671-61312690 None:intergenic 55.0%
GTGAGTGGAACAGGGTTCTC+TGG - chr1_2:61312679-61312698 None:intergenic 55.0%
TGCCCAATCCTGCCTGCTTC+TGG + chr1_2:61311080-61311099 Msa0082460:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 61310400 61312937 61310400 ID=Msa0082460;Name=Msa0082460
chr1_2 mRNA 61310400 61312937 61310400 ID=Msa0082460-mRNA-1;Parent=Msa0082460;Name=Msa0082460-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0.5|0.66|0.66|1|0.66|3|1597|119
chr1_2 exon 61310400 61310404 61310400 ID=Msa0082460-mRNA-1:exon:20289;Parent=Msa0082460-mRNA-1
chr1_2 exon 61310545 61312035 61310545 ID=Msa0082460-mRNA-1:exon:20290;Parent=Msa0082460-mRNA-1
chr1_2 exon 61312477 61312937 61312477 ID=Msa0082460-mRNA-1:exon:20291;Parent=Msa0082460-mRNA-1
chr1_2 CDS 61310400 61310404 61310400 ID=Msa0082460-mRNA-1:cds;Parent=Msa0082460-mRNA-1
chr1_2 CDS 61310545 61310899 61310545 ID=Msa0082460-mRNA-1:cds;Parent=Msa0082460-mRNA-1
chr1_2 three_prime_UTR 61310900 61312035 61310900 ID=Msa0082460-mRNA-1:three_prime_utr;Parent=Msa0082460-mRNA-1
chr1_2 three_prime_UTR 61312477 61312937 61312477 ID=Msa0082460-mRNA-1:three_prime_utr;Parent=Msa0082460-mRNA-1
Gene Sequence

>Msa0082460

ATGAAGGGATACAAGAGATTTTTTAAGAGAACGTCATTAGAGACTTCAGATTACCTTAAAGATAACTGTCTTAGTGTTAATTGTAGCGTTGGTGTTGTGAGGTCACACACAGAGGGCCCTAGAACATTTTCGATTCCAATATCACCTTCTACTATTGGTCACCAGTTTGGGAAGCTTTTGGAAAGTGGAAAAAGCAGCGATGTGAATTTTGAAGTGAATGGTGAAATTTTTGCGGCTCATAAATTGGTACTAGCAGCTCGATCACCTGTTTTTAGAGCTCAGCTTTTTGGTCCTATGAAAGATCAGAACACACAGTGTATAAAAGTTGAAGATATCGAGGCTCCTGTTTTCAAGGTTTAA
Protein sequence

>Msa0082460

MKGYKRFFKRTSLETSDYLKDNCLSVNCSVGVVRSHTEGPRTFSIPISPSTIGHQFGKLLESGKSSDVNFEVNGEIFAAHKLVLAARSPVFRAQLFGPMKDQNTQCIKVEDIEAPVFKV*