AlfalfaGEDB | Msa0082660

M. sativa cultivar zhongmu-4 / Msa0082660

KEGG: -
GO: GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0010065 GO:0010067 GO:0010087 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048731 GO:0048856 GO:0061458
PFAM: PF12799.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0082660	sp\|C0LGP4\|Y3475_ARATH	42.098	753	343	13	5	680	258	994	0.0	550
Msa0082660	sp\|C0LGP4\|Y3475_ARATH	28.612	353	171	11	4	296	160	491	1.03e-21	104
Msa0082660	sp\|C0LGP4\|Y3475_ARATH	29.446	343	170	13	16	303	100	425	5.40e-19	95.5
Msa0082660	sp\|C0LGP4\|Y3475_ARATH	30.252	238	144	7	81	308	85	310	7.84e-15	82.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0082660	A0A396JS71	28.829	333	182	10	2	297	183	497	3.47e-18	101
Msa0082660	A0A396JS71	29.143	175	116	3	107	281	111	277	3.01e-11	79.0
Msa0082660	A0A396JS71	77.646	756	102	3	1	689	303	1058	0.0	1120

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0082660	MtrunA17_Chr1g0196031	77.646	756	102	3	1	689	303	1058	0.0	1120
Msa0082660	MtrunA17_Chr1g0196031	28.829	333	182	10	2	297	183	497	6.69e-22	101
Msa0082660	MtrunA17_Chr1g0196031	29.143	175	116	3	107	281	111	277	5.80e-15	79.0
Msa0082660	MtrunA17_Chr1g0196011	73.976	757	111	6	1	689	112	850	0.0	1080
Msa0082660	MtrunA17_Chr1g0196011	30.693	303	175	10	13	297	3	288	5.04e-22	101
Msa0082660	MtrunA17_Chr1g0196001	78.976	723	83	5	1	656	265	985	0.0	1053
Msa0082660	MtrunA17_Chr1g0196001	35.912	181	107	4	100	280	91	262	6.49e-19	91.7
Msa0082660	MtrunA17_Chr1g0196001	26.061	330	193	10	8	303	127	439	5.45e-18	89.0
Msa0082660	MtrunA17_Chr1g0196001	26.367	311	182	5	2	280	145	440	1.99e-17	87.0
Msa0082660	MtrunA17_Chr6g0464171	52.306	759	281	13	1	687	272	1021	0.0	734

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0082660	AT3G47570.1	42.098	753	343	13	5	680	258	994	0.0	550
Msa0082660	AT3G47570.1	28.612	353	171	11	4	296	160	491	1.05e-22	104
Msa0082660	AT3G47570.1	29.446	343	170	13	16	303	100	425	5.49e-20	95.5
Msa0082660	AT3G47570.1	30.252	238	144	7	81	308	85	310	7.97e-16	82.4
Msa0082660	AT3G47570.1	31.293	147	99	2	107	253	62	206	8.73e-11	65.9
Msa0082660	AT3G47580.1	38.992	754	366	13	5	680	258	995	1.51e-165	502
Msa0082660	AT3G47580.1	28.620	297	179	9	8	280	116	403	1.60e-15	81.3
Msa0082660	AT3G47580.1	26.693	251	150	3	16	266	100	316	9.63e-15	79.0
Msa0082660	AT3G47090.1	39.442	753	362	15	5	680	258	993	2.92e-165	501
Msa0082660	AT3G47090.1	26.861	309	186	6	4	296	160	444	3.50e-20	96.3

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Find 136 sgRNAs with CRISPR-Local

Find 364 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATTAGAAATTGTTAG+TGG	0.414376	1_2:+61478246	Msa0082660:CDS
AAAATTGCCTAAGATTTGGG+AGG	0.609428	1_2:-61476604	None:intergenic
AAACTCCCAGTCATCACCAC+TGG	0.569122	1_2:-61476804	None:intergenic
AAATGTCAGGCTTTGCGGTG+GGG	0.586864	1_2:+61477669	Msa0082660:CDS
AAATTGCCTAAGATTTGGGA+GGG	0.595859	1_2:-61476603	None:intergenic
AACAATTGAGCAACTTCCAA+AGG	0.546860	1_2:+61477873	Msa0082660:CDS
AACCAGCTCAATGGGAATAA+AGG	0.317409	1_2:+61478434	Msa0082660:CDS
AACTGCACCATGCCACTGAT+GGG	0.540282	1_2:+61477908	Msa0082660:CDS
AAGAGAAAGAATCAATATCA+AGG	0.520373	1_2:+61478877	Msa0082660:CDS
AAGGCGACATGTACAGCTTT+GGG	0.422299	1_2:+61478603	Msa0082660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTGGAATAACATCAGTTAAA+AGG	-	chr1_2:61477030-61477049	None:intergenic	30.0%
TGAAACTTAGAAAAACTCTC+TGG	-	chr1_2:61477049-61477068	None:intergenic	30.0%
AGATTTCCTAAAAAAGTCAC+TGG	-	chr1_2:61477121-61477140	None:intergenic	30.0%
TTGCAATTTCCAATAGTTGA+AGG	-	chr1_2:61477195-61477214	None:intergenic	30.0%
CACTTAATGAGTTATGTGAT+AGG	-	chr1_2:61477311-61477330	None:intergenic	30.0%
CTATCACATAACTCATTAAG+TGG	+	chr1_2:61477309-61477328	Msa0082660:CDS	30.0%
ATATCTCAAAATCATCTCTC+CGG	+	chr1_2:61477381-61477400	Msa0082660:CDS	30.0%
TTAGAGGGTAATTCTTTCAA+TGG	+	chr1_2:61477453-61477472	Msa0082660:intron	30.0%
TCTATCAGATAATGAGGAAA+AGG	+	chr1_2:61477827-61477846	Msa0082660:CDS	30.0%
TTGCTCAATTGTTGAATCAA+GGG	-	chr1_2:61477866-61477885	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	61476593	61478946	61476593	ID=Msa0082660;Name=Msa0082660
chr1_2	mRNA	61476593	61478946	61476593	ID=Msa0082660-mRNA-1;Parent=Msa0082660;Name=Msa0082660-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0\|0\|0\|1\|1\|1\|4\|0\|689
chr1_2	exon	61476593	61477062	61476593	ID=Msa0082660-mRNA-1:exon:20313;Parent=Msa0082660-mRNA-1
chr1_2	exon	61477134	61477459	61477134	ID=Msa0082660-mRNA-1:exon:20314;Parent=Msa0082660-mRNA-1
chr1_2	exon	61477581	61478480	61477581	ID=Msa0082660-mRNA-1:exon:20315;Parent=Msa0082660-mRNA-1
chr1_2	exon	61478573	61478946	61478573	ID=Msa0082660-mRNA-1:exon:20316;Parent=Msa0082660-mRNA-1
chr1_2	CDS	61476593	61477062	61476593	ID=Msa0082660-mRNA-1:cds;Parent=Msa0082660-mRNA-1
chr1_2	CDS	61477134	61477459	61477134	ID=Msa0082660-mRNA-1:cds;Parent=Msa0082660-mRNA-1
chr1_2	CDS	61477581	61478480	61477581	ID=Msa0082660-mRNA-1:cds;Parent=Msa0082660-mRNA-1
chr1_2	CDS	61478573	61478946	61478573	ID=Msa0082660-mRNA-1:cds;Parent=Msa0082660-mRNA-1

Gene Sequence

>Msa0082660

ATGTTCACAACCCTCCCAAATCTTAGGCAATTTTGGGTTGGTTCGAATCGATTCTCAGGTCCGATCCCAACATCCATCAGTAATGCATCTCGGATTCAAATGTTCGATATTGGTTTTAACAATTTTGTAGGACGAATTCCAAATCTTGGAAAGCTACAAGATCTTTCTGTTTTGGCTGTAGGCGAAAATAATCTTGGCAGCAATTCGTCATCCAGTGGTGATGACTGGGAGTTTATAAAGTCTTTGGTAAACTGTAGTAAGCTTGATATAGTAATTGTTGAACGTAATTACTTTGGAGGTCCTTTGCCCAAAATTATTGGCAATTTGTCCACCCATCTTTCACAACTTGCTATGGCTGACAATCAGATATCTGGAGAAATTCCAACAGAACTCGGAAATCTTGTAAACTTGATTTTCTTGTCCATGGCTAACAACCTTTTAACTGATGTTATTCCAGAGAGTTTTTCTAAGAAATCTCAGTCACTTATCTCAATTGCATTTATCAAACATTTGTTGACTGGAAAGATTCCTTCAACTATTGGAAATTGCAAAATGTTGCAAGCAGTAGACTTTTCAATGAACAACCTTAGTGGAACTATACCAACACAACTTCTTAGCCTTTCCTACTTATCACTTGTGCTCAACCTATCACATAACTCATTAAGTGGTAATTTTCCTACTGAGGTGGGCATGCTTCAAACCATTGGGATGCTTGATATATCTCAAAATCATCTCTCCGGTGGCATTCCTGAAAATATCGGAGATTGCTTAAGCTTAGAGTACCTATTTTTAGAGGAATGGTTCAATGCTTCTTTTAACAAGTTGGATGGTGAGGTGCCCATGCTTGGCGTTTTCCAGAATGCTAGTAGGGTTTCATTGAGTGGAAATGTCAGGCTTTGCGGTGGGGTAGCAAAACTGAATTTGAAAGTTTGCCCACCAAAAGATGTGAAAAAGAGAGAACATATTCGCCGCAAGTTGATCATCGTCATCATCTTCTCAACTGTATCTCTTCTTCTATTGTTATTTGTCCTTACTATCGTCATCTATCAGATAATGAGGAAAAGGCAGAGGAAAGCAACCCTTGATTCAACAATTGAGCAACTTCCAAAGGTATCATACCAAGAACTGCACCATGCCACTGATGGGTTCTCGGTTGGGAACTTGGTAGGAATTGGAGGTACGGGTTCTGTGTACAAAGGAGGACTTAATTCAGAAGAAAGAGTTGTTGCTGTAAAGGTTCTAAACCTTCGGAAGAAAGGAGCTCAGAAAAGTTTCATTGCCGAATGTAATGCTTTCAGAAATATCAGGCATAGAAATCTAGTCAAGATTATCACATGTTGCTCTAGTGTGGATCACAAAGGTAATGACTTTAAAGCAATTGTTTTTGAATACATGACAAATGGGAGCTTAGAGGAATGGTTGCATCGAAATGAAGAACAACATAGAACACTGAAATTCGAAAAAAGATTAGAAATTGTTAGTGGAATTGCTTCTGCATTACACTATCTTCACTATGAATGCGAGAAGCCTATTGTTCATTGTGATTTGAAACCAAGCAATGTCCTTCTTGATGATGACATGGTTGCTCATGTGAGTGATTTTGGCTTAGCCAGACTTGTTTCAACAATTGATAGGAACTCCAATAAACAAACCAGCTCAATGGGAATAAAGGGGACTATAGGTTATACTCCTCCAGAGTATGGAATGGATTCTCAATTGTCAACAGAAGGCGACATGTACAGCTTTGGGATTCTTCTTTTGGAGATGTTGACTGGGAGAAGACCAACTGATGCAATGTTTAAAGATGGTCACAACCTCCACAACTACGTGAAAATTGCATTTCCAAATGACATTTTGGAGATTGTTGATGCAACTCTCTTCTCAGAGGAAAATGACCTTCTTGCAGTTACAACAGAGGTTGCAAGTGATTTGCATCCCAATGTTGAAAGGTGCTTATCTTCTTTATTTAACATTGGATTATCTTGTTCAGTGGAGTCTCCAAGAGAAAGAATCAATATCAAGGAAGTCATCGTGGAACTCAATATAATTTCAAAAGCTTTGGCTGATTAA

Protein sequence

>Msa0082660

MFTTLPNLRQFWVGSNRFSGPIPTSISNASRIQMFDIGFNNFVGRIPNLGKLQDLSVLAVGENNLGSNSSSSGDDWEFIKSLVNCSKLDIVIVERNYFGGPLPKIIGNLSTHLSQLAMADNQISGEIPTELGNLVNLIFLSMANNLLTDVIPESFSKKSQSLISIAFIKHLLTGKIPSTIGNCKMLQAVDFSMNNLSGTIPTQLLSLSYLSLVLNLSHNSLSGNFPTEVGMLQTIGMLDISQNHLSGGIPENIGDCLSLEYLFLEEWFNASFNKLDGEVPMLGVFQNASRVSLSGNVRLCGGVAKLNLKVCPPKDVKKREHIRRKLIIVIIFSTVSLLLLLFVLTIVIYQIMRKRQRKATLDSTIEQLPKVSYQELHHATDGFSVGNLVGIGGTGSVYKGGLNSEERVVAVKVLNLRKKGAQKSFIAECNAFRNIRHRNLVKIITCCSSVDHKGNDFKAIVFEYMTNGSLEEWLHRNEEQHRTLKFEKRLEIVSGIASALHYLHYECEKPIVHCDLKPSNVLLDDDMVAHVSDFGLARLVSTIDRNSNKQTSSMGIKGTIGYTPPEYGMDSQLSTEGDMYSFGILLLEMLTGRRPTDAMFKDGHNLHNYVKIAFPNDILEIVDATLFSEENDLLAVTTEVASDLHPNVERCLSSLFNIGLSCSVESPRERINIKEVIVELNIISKALAD*