Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0083840 | GAU32342.1 | 58.756 | 434 | 149 | 9 | 11 | 429 | 22 | 440 | 9.65e-159 | 465 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0083840 | sp|Q9SRH9|SNL1_ARATH | 33.540 | 161 | 88 | 5 | 8 | 164 | 56 | 201 | 1.03e-14 | 80.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0083840 | A0A2Z6N6C3 | 58.756 | 434 | 149 | 9 | 11 | 429 | 22 | 440 | 4.61e-159 | 465 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0083840 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0083840 | MtrunA17_Chr3g0093911 | 68.732 | 355 | 104 | 4 | 77 | 429 | 1 | 350 | 6.54e-162 | 459 |
| Msa0083840 | MtrunA17_Chr7g0252101 | 60.941 | 425 | 151 | 11 | 12 | 429 | 16 | 432 | 4.02e-159 | 456 |
| Msa0083840 | MtrunA17_Chr1g0207351 | 64.607 | 356 | 114 | 7 | 81 | 429 | 16 | 366 | 8.70e-143 | 412 |
| Msa0083840 | MtrunA17_Chr4g0075971 | 53.881 | 438 | 176 | 8 | 10 | 429 | 173 | 602 | 1.09e-130 | 389 |
| Msa0083840 | MtrunA17_Chr6g0488101 | 48.359 | 457 | 187 | 15 | 11 | 429 | 10 | 455 | 2.71e-120 | 358 |
| Msa0083840 | MtrunA17_Chr3g0077801 | 54.775 | 356 | 154 | 5 | 77 | 429 | 1 | 352 | 8.25e-118 | 347 |
| Msa0083840 | MtrunA17_Chr6g0488271 | 49.522 | 418 | 170 | 13 | 43 | 429 | 8 | 415 | 1.34e-114 | 342 |
| Msa0083840 | MtrunA17_Chr3g0077901 | 50.000 | 362 | 168 | 5 | 73 | 428 | 17 | 371 | 2.06e-108 | 324 |
| Msa0083840 | MtrunA17_Chr1g0209051 | 62.545 | 275 | 94 | 4 | 158 | 428 | 1 | 270 | 3.39e-107 | 317 |
| Msa0083840 | MtrunA17_Chr3g0077851 | 52.507 | 339 | 148 | 5 | 96 | 428 | 4 | 335 | 5.50e-107 | 319 |
| Msa0083840 | MtrunA17_Chr3g0112311 | 51.657 | 362 | 157 | 9 | 77 | 427 | 1 | 355 | 9.00e-104 | 312 |
| Msa0083840 | MtrunA17_Chr7g0243331 | 52.422 | 351 | 158 | 7 | 82 | 429 | 6 | 350 | 6.35e-103 | 310 |
| Msa0083840 | MtrunA17_Chr6g0452641 | 50.159 | 315 | 138 | 9 | 127 | 429 | 33 | 340 | 4.07e-77 | 243 |
| Msa0083840 | MtrunA17_Chr3g0122991 | 44.000 | 325 | 118 | 7 | 79 | 391 | 2 | 274 | 1.82e-71 | 226 |
| Msa0083840 | MtrunA17_Chr1g0202841 | 63.536 | 181 | 64 | 1 | 249 | 429 | 1 | 179 | 2.70e-70 | 219 |
| Msa0083840 | MtrunA17_Chr3g0123471 | 49.254 | 268 | 123 | 8 | 158 | 421 | 1 | 259 | 4.57e-62 | 201 |
| Msa0083840 | MtrunA17_Chr4g0075861 | 53.202 | 203 | 78 | 3 | 10 | 197 | 173 | 373 | 1.46e-51 | 177 |
| Msa0083840 | MtrunA17_Chr7g0243391 | 45.794 | 214 | 111 | 3 | 73 | 283 | 14 | 225 | 9.20e-51 | 171 |
| Msa0083840 | MtrunA17_Chr1g0206951 | 62.393 | 117 | 43 | 1 | 80 | 195 | 2 | 118 | 2.31e-41 | 143 |
| Msa0083840 | MtrunA17_Chr1g0187941 | 57.664 | 137 | 58 | 0 | 249 | 385 | 1 | 137 | 8.84e-41 | 142 |
| Msa0083840 | MtrunA17_Chr1g0196231 | 56.410 | 156 | 61 | 4 | 65 | 217 | 215 | 366 | 3.69e-40 | 147 |
| Msa0083840 | MtrunA17_Chr6g0486591 | 47.239 | 163 | 73 | 5 | 276 | 429 | 1 | 159 | 1.09e-34 | 126 |
| Msa0083840 | MtrunA17_Chr1g0209191 | 59.406 | 101 | 35 | 2 | 331 | 428 | 1 | 98 | 3.84e-30 | 112 |
| Msa0083840 | MtrunA17_Chr1g0209061 | 53.846 | 117 | 42 | 3 | 1 | 116 | 2 | 107 | 5.25e-29 | 109 |
| Msa0083840 | MtrunA17_Chr1g0196241 | 69.620 | 79 | 20 | 2 | 322 | 399 | 3 | 78 | 6.91e-28 | 105 |
| Msa0083840 | MtrunA17_Chr6g0459061 | 29.143 | 350 | 228 | 11 | 85 | 426 | 18 | 355 | 1.43e-27 | 112 |
| Msa0083840 | MtrunA17_Chr7g0238781 | 42.222 | 135 | 71 | 5 | 109 | 239 | 27 | 158 | 7.72e-18 | 80.5 |
| Msa0083840 | MtrunA17_Chr8g0364231 | 39.831 | 118 | 64 | 3 | 8 | 125 | 41 | 151 | 4.09e-17 | 84.3 |
| Msa0083840 | MtrunA17_Chr1g0206781 | 47.500 | 80 | 36 | 2 | 11 | 85 | 13 | 91 | 2.25e-15 | 72.4 |
| Msa0083840 | MtrunA17_Chr3g0077911 | 64.615 | 65 | 21 | 2 | 9 | 72 | 90 | 153 | 2.37e-15 | 73.6 |
| Msa0083840 | MtrunA17_Chr1g0205861 | 46.154 | 78 | 41 | 1 | 8 | 85 | 15 | 91 | 6.48e-15 | 70.9 |
| Msa0083840 | MtrunA17_Chr1g0209201 | 51.351 | 74 | 34 | 2 | 5 | 78 | 12 | 83 | 7.19e-15 | 71.6 |
| Msa0083840 | MtrunA17_Chr5g0415031 | 40.385 | 104 | 55 | 3 | 8 | 111 | 42 | 138 | 1.15e-14 | 76.6 |
| Msa0083840 | MtrunA17_Chr3g0077861 | 63.077 | 65 | 22 | 2 | 9 | 72 | 90 | 153 | 2.20e-14 | 70.9 |
| Msa0083840 | MtrunA17_Chr6g0452001 | 35.593 | 118 | 69 | 2 | 8 | 125 | 62 | 172 | 1.61e-13 | 73.2 |
| Msa0083840 | MtrunA17_Chr6g0452021 | 35.897 | 117 | 66 | 3 | 9 | 125 | 43 | 150 | 3.67e-13 | 71.2 |
| Msa0083840 | MtrunA17_Chr5g0431721 | 55.385 | 65 | 25 | 3 | 13 | 74 | 16 | 79 | 1.35e-12 | 64.3 |
| Msa0083840 | MtrunA17_Chr7g0218911 | 46.774 | 62 | 32 | 1 | 13 | 74 | 2 | 62 | 4.58e-12 | 62.8 |
| Msa0083840 | MtrunA17_Chr1g0206771 | 48.438 | 64 | 32 | 1 | 11 | 74 | 18 | 80 | 5.47e-12 | 64.3 |
| Msa0083840 | MtrunA17_Chr1g0206771 | 46.377 | 69 | 36 | 1 | 4 | 72 | 90 | 157 | 1.32e-11 | 63.2 |
| Msa0083840 | MtrunA17_Chr3g0077881 | 47.312 | 93 | 39 | 4 | 1 | 92 | 1 | 84 | 9.65e-12 | 63.5 |
| Msa0083840 | MtrunA17_Chr3g0077891 | 50.633 | 79 | 36 | 3 | 1 | 78 | 1 | 77 | 1.39e-11 | 63.2 |
| Msa0083840 | MtrunA17_Chr3g0077941 | 50.633 | 79 | 36 | 3 | 1 | 78 | 1 | 77 | 1.47e-11 | 62.8 |
| Msa0083840 | MtrunA17_Chr3g0077941 | 53.846 | 65 | 28 | 2 | 9 | 72 | 98 | 161 | 2.26e-11 | 62.4 |
| Msa0083840 | MtrunA17_Chr8g0364021 | 43.038 | 79 | 44 | 1 | 8 | 86 | 43 | 120 | 2.79e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0083840 | AT3G01320.2 | 33.540 | 161 | 88 | 5 | 8 | 164 | 56 | 201 | 9.79e-16 | 80.5 |
| Msa0083840 | AT3G01320.1 | 33.540 | 161 | 88 | 5 | 8 | 164 | 56 | 201 | 1.04e-15 | 80.1 |
| Msa0083840 | AT1G24190.3 | 41.414 | 99 | 51 | 2 | 8 | 106 | 13 | 104 | 3.38e-15 | 78.6 |
| Msa0083840 | AT1G24190.1 | 46.914 | 81 | 42 | 1 | 8 | 88 | 13 | 92 | 3.41e-15 | 78.6 |
| Msa0083840 | AT1G24190.2 | 41.414 | 99 | 51 | 2 | 8 | 106 | 13 | 104 | 3.62e-15 | 78.6 |
| Msa0083840 | AT5G15020.1 | 36.975 | 119 | 64 | 3 | 8 | 125 | 51 | 159 | 6.44e-15 | 77.8 |
| Msa0083840 | AT5G15020.2 | 36.975 | 119 | 64 | 3 | 8 | 125 | 51 | 159 | 7.53e-15 | 77.4 |
| Msa0083840 | AT1G59890.4 | 46.988 | 83 | 41 | 2 | 9 | 89 | 45 | 126 | 3.58e-14 | 75.5 |
| Msa0083840 | AT1G59890.1 | 46.988 | 83 | 41 | 2 | 9 | 89 | 45 | 126 | 3.85e-14 | 75.5 |
| Msa0083840 | AT1G59890.2 | 46.988 | 83 | 41 | 2 | 9 | 89 | 45 | 126 | 3.99e-14 | 75.1 |
| Msa0083840 | AT1G59890.3 | 46.988 | 83 | 41 | 2 | 9 | 89 | 45 | 126 | 4.06e-14 | 75.1 |
| Msa0083840 | AT1G70060.2 | 52.239 | 67 | 31 | 1 | 8 | 74 | 13 | 78 | 1.26e-13 | 73.6 |
| Msa0083840 | AT1G70060.1 | 52.239 | 67 | 31 | 1 | 8 | 74 | 13 | 78 | 1.26e-13 | 73.6 |
| Msa0083840 | AT5G15025.1 | 44.737 | 76 | 41 | 1 | 8 | 83 | 46 | 120 | 1.96e-13 | 67.4 |
| Msa0083840 | AT1G10450.2 | 50.725 | 69 | 33 | 1 | 9 | 77 | 33 | 100 | 2.63e-12 | 69.7 |
| Msa0083840 | AT1G10450.1 | 50.725 | 69 | 33 | 1 | 9 | 77 | 33 | 100 | 2.63e-12 | 69.7 |
| Msa0083840 | AT1G10450.3 | 50.725 | 69 | 33 | 1 | 9 | 77 | 84 | 151 | 2.98e-12 | 69.3 |
| Msa0083840 | AT1G24200.1 | 49.254 | 67 | 33 | 1 | 8 | 74 | 13 | 78 | 3.44e-12 | 65.5 |
| Msa0083840 | AT1G27250.1 | 43.662 | 71 | 39 | 1 | 8 | 78 | 20 | 89 | 1.46e-11 | 62.4 |
| Msa0083840 | AT1G70030.3 | 37.755 | 98 | 53 | 2 | 9 | 106 | 22 | 111 | 1.46e-11 | 62.0 |
| Msa0083840 | AT1G70030.2 | 37.755 | 98 | 53 | 2 | 9 | 106 | 22 | 111 | 2.47e-11 | 61.6 |
| Msa0083840 | AT1G70030.1 | 36.893 | 103 | 57 | 2 | 9 | 111 | 11 | 105 | 8.68e-11 | 60.8 |
Find 92 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCACTCGATTTATTTCT+TGG | 0.092423 | 1_2:+62994900 | Msa0083840:five_prime_UTR |
| TTCCAGCCCTCAAATCTTTC+AGG | 0.146764 | 1_2:-62995100 | None:intergenic |
| TTATTTGTAGTTTCTGAATT+AGG | 0.165086 | 1_2:+62994790 | Msa0083840:five_prime_UTR |
| TTCTACATTAGTCAAATTAT+TGG | 0.171653 | 1_2:-62997572 | None:intergenic |
| GATTTCATTATTCTTGTAAA+TGG | 0.220120 | 1_2:-62996783 | None:intergenic |
| CTCTCATATCCCCACTTGTT+CGG | 0.274296 | 1_2:-62997299 | None:intergenic |
| ATTAATAGTTTGAGTTCTTT+TGG | 0.277321 | 1_2:+62997658 | Msa0083840:three_prime_UTR |
| TATTTGTAGTTTCTGAATTA+GGG | 0.283688 | 1_2:+62994791 | Msa0083840:five_prime_UTR |
| AGGACATACAGATTTAATTC+TGG | 0.289730 | 1_2:+62995172 | Msa0083840:CDS |
| TTCAGAAACTACAAATAAAA+AGG | 0.297870 | 1_2:-62994785 | None:intergenic |
| CAAATTATTGGCAAACAAAA+AGG | 0.304170 | 1_2:-62997560 | None:intergenic |
| CTCTGAGGAATTTGTCTTTG+TGG | 0.313965 | 1_2:+62996872 | Msa0083840:CDS |
| TTTCAGTCTGACATAATTCA+TGG | 0.325552 | 1_2:-62997207 | None:intergenic |
| TGTTGTGATAAAAGGGAAAA+AGG | 0.329108 | 1_2:+62996425 | Msa0083840:intron |
| TATGAAGAAGTAATGGTTCT+TGG | 0.336847 | 1_2:-62996599 | None:intergenic |
| AATCGAGTGAAGGAGGATTA+AGG | 0.348459 | 1_2:-62994890 | None:intergenic |
| TCTCATATCCCCACTTGTTC+GGG | 0.349130 | 1_2:-62997298 | None:intergenic |
| GTACACATTTGCACTTTCAT+TGG | 0.360934 | 1_2:-62997168 | None:intergenic |
| GGACATACAGATTTAATTCT+GGG | 0.368970 | 1_2:+62995173 | Msa0083840:CDS |
| AGTTTATCACTTGGAGATGA+TGG | 0.375141 | 1_2:+62996674 | Msa0083840:CDS |
| TTTCCGGCGTGTGCAAGAAT+TGG | 0.380354 | 1_2:+62996535 | Msa0083840:CDS |
| TTGGTTGGGTATGCTAATAA+AGG | 0.390018 | 1_2:-62996649 | None:intergenic |
| AGAGAACTATGAACTTCCTT+TGG | 0.396409 | 1_2:+62996461 | Msa0083840:CDS |
| ACACGCCGGAAAACTGAAAT+AGG | 0.402270 | 1_2:-62996524 | None:intergenic |
| ATCACTAACGAGGCGAAGAT+AGG | 0.404482 | 1_2:+62997020 | Msa0083840:CDS |
| TAAAACTCCTGAAAGATTTG+AGG | 0.415830 | 1_2:+62995093 | Msa0083840:CDS |
| AAAACTCCTGAAAGATTTGA+GGG | 0.424484 | 1_2:+62995094 | Msa0083840:CDS |
| CTGAGGAGTACTCCCGTCAT+AGG | 0.433963 | 1_2:+62997083 | Msa0083840:CDS |
| CGAATCGGCGACGATAAACT+AGG | 0.434109 | 1_2:+62995011 | Msa0083840:CDS |
| CTAGTTTATCGTCGCCGATT+CGG | 0.442166 | 1_2:-62995010 | None:intergenic |
| TATGTCAGACTGAAAACTTT+GGG | 0.448246 | 1_2:+62997215 | Msa0083840:CDS |
| CCGGCGTGTGCAAGAATTGG+AGG | 0.450628 | 1_2:+62996538 | Msa0083840:CDS |
| TAGGAATTCCTAGTTCCCTT+AGG | 0.453013 | 1_2:+62997102 | Msa0083840:CDS |
| ATGAAGAAGTAATGGTTCTT+GGG | 0.453465 | 1_2:-62996598 | None:intergenic |
| CTCCTGAAAGATTTGAGGGC+TGG | 0.459564 | 1_2:+62995098 | Msa0083840:CDS |
| ATTGTACATTGCGTTACTCT+TGG | 0.461731 | 1_2:+62997516 | Msa0083840:three_prime_UTR |
| TTATGTCAGACTGAAAACTT+TGG | 0.462565 | 1_2:+62997214 | Msa0083840:CDS |
| CAATATGTAGCGTTTATTCA+AGG | 0.467145 | 1_2:+62997354 | Msa0083840:CDS |
| GGATGATATTCAAGAAGAGA+TGG | 0.468259 | 1_2:-62997410 | None:intergenic |
| GCGTTCCAACCAACAGTTGT+AGG | 0.477939 | 1_2:-62997431 | None:intergenic |
| AACTATGCAAATCAGTTCAA+TGG | 0.478283 | 1_2:+62994861 | Msa0083840:five_prime_UTR |
| GAATAATAACTCTTAGCATA+AGG | 0.482018 | 1_2:-62996627 | None:intergenic |
| CGTATCAAAATTAAATGTGT+TGG | 0.485240 | 1_2:+62997633 | Msa0083840:three_prime_UTR |
| GAATATCATCCTACAACTGT+TGG | 0.486533 | 1_2:+62997422 | Msa0083840:CDS |
| GTCTATCAATCTCGAAATTG+TGG | 0.493729 | 1_2:+62996921 | Msa0083840:CDS |
| AGAGTGGGTTGTTGACTTTG+TGG | 0.494217 | 1_2:+62996974 | Msa0083840:CDS |
| ATAACCAACCTAAGGGAACT+AGG | 0.496475 | 1_2:-62997110 | None:intergenic |
| CATCACAAATAACCAACCTA+AGG | 0.498767 | 1_2:-62997118 | None:intergenic |
| TCACTAACGAGGCGAAGATA+GGG | 0.506057 | 1_2:+62997021 | Msa0083840:CDS |
| AATTCCTAGTTCCCTTAGGT+TGG | 0.508434 | 1_2:+62997106 | Msa0083840:CDS |
| GGAAGAAGAATTGATATAAG+AGG | 0.508673 | 1_2:+62995119 | Msa0083840:CDS |
| GAAGAGTTACCTTCAGAGGT+AGG | 0.509259 | 1_2:-62996813 | None:intergenic |
| AAGAGTTACCTTCAGAGGTA+GGG | 0.509565 | 1_2:-62996812 | None:intergenic |
| AATATGTAGCGTTTATTCAA+GGG | 0.510138 | 1_2:+62997355 | Msa0083840:CDS |
| TAGATGCCTAAAACACCAAT+CGG | 0.521949 | 1_2:-62997453 | None:intergenic |
| ATCAATCTCGAAATTGTGGA+TGG | 0.526711 | 1_2:+62996925 | Msa0083840:CDS |
| GTTGAACGAGCAGCCGTGGA+AGG | 0.529951 | 1_2:-62994841 | None:intergenic |
| TAAAGAAGAGTTACCTTCAG+AGG | 0.531919 | 1_2:-62996817 | None:intergenic |
| AGAGTTACCTTCAGAGGTAG+GGG | 0.532859 | 1_2:-62996811 | None:intergenic |
| ATCATCCTACAACTGTTGGT+TGG | 0.540075 | 1_2:+62997426 | Msa0083840:CDS |
| CATAGTTGAACGAGCAGCCG+TGG | 0.548445 | 1_2:-62994845 | None:intergenic |
| GGCGATATATGAAGAAGTAA+TGG | 0.551671 | 1_2:-62996606 | None:intergenic |
| AAGATGAAGAATTTCCGAAT+CGG | 0.560007 | 1_2:+62994996 | Msa0083840:exon |
| AACAATAGAAAATAGAGAGT+GGG | 0.566020 | 1_2:+62996959 | Msa0083840:CDS |
| GATGATATTCAAGAAGAGAT+GGG | 0.570526 | 1_2:-62997409 | None:intergenic |
| CACTCGAATCATCCCTTCCA+CGG | 0.572095 | 1_2:+62994828 | Msa0083840:five_prime_UTR |
| AGAAATAAATCGAGTGAAGG+AGG | 0.577568 | 1_2:-62994897 | None:intergenic |
| CAACAATAGAAAATAGAGAG+TGG | 0.582774 | 1_2:+62996958 | Msa0083840:CDS |
| AGAGTGAAGAAGCTGTTGAA+AGG | 0.582834 | 1_2:+62995152 | Msa0083840:CDS |
| GCATTTGCTAAAAGCAAGCA+AGG | 0.585085 | 1_2:-62996850 | None:intergenic |
| CTGTTGGTTGGAACGCCGAT+TGG | 0.585750 | 1_2:+62997438 | Msa0083840:CDS |
| CATTGAGCAACCCGAACAAG+TGG | 0.585822 | 1_2:+62997288 | Msa0083840:CDS |
| TTGAACGAGCAGCCGTGGAA+GGG | 0.593904 | 1_2:-62994840 | None:intergenic |
| GAACTAGGAATTCCTATGAC+GGG | 0.594218 | 1_2:-62997095 | None:intergenic |
| CCAAGAAATAAATCGAGTGA+AGG | 0.596858 | 1_2:-62994900 | None:intergenic |
| GTGATATCAAACATATCCAA+AGG | 0.599034 | 1_2:-62996477 | None:intergenic |
| ATCACAAATAACCAACCTAA+GGG | 0.609657 | 1_2:-62997117 | None:intergenic |
| GTAGTAACTAACCAGTTGGA+AGG | 0.611566 | 1_2:-62995208 | None:intergenic |
| ATATTAAATGTCTTAATCTG+AGG | 0.614293 | 1_2:+62997066 | Msa0083840:CDS |
| TCAATCTCGAAATTGTGGAT+GGG | 0.618953 | 1_2:+62996926 | Msa0083840:CDS |
| GAATCGGCGACGATAAACTA+GGG | 0.619068 | 1_2:+62995012 | Msa0083840:CDS |
| GGAACTAGGAATTCCTATGA+CGG | 0.624231 | 1_2:-62997096 | None:intergenic |
| CACTAACGAGGCGAAGATAG+GGG | 0.627150 | 1_2:+62997022 | Msa0083840:CDS |
| CGATAAACTAGGGTTTCTCA+AGG | 0.627978 | 1_2:+62995022 | Msa0083840:CDS |
| AATATGTAACATAGCTAGAG+CGG | 0.634773 | 1_2:-62996707 | None:intergenic |
| ATTGAGCAACCCGAACAAGT+GGG | 0.652805 | 1_2:+62997289 | Msa0083840:CDS |
| CCTCCAATTCTTGCACACGC+CGG | 0.653779 | 1_2:-62996538 | None:intergenic |
| AGCAACTCTGTGTATTACAT+TGG | 0.654518 | 1_2:+62997320 | Msa0083840:CDS |
| TTGAGCAACCCGAACAAGTG+GGG | 0.657706 | 1_2:+62997290 | Msa0083840:CDS |
| AGCTATGTTACATATTCTAG+CGG | 0.658771 | 1_2:+62996714 | Msa0083840:CDS |
| GAGTTACCTTCAGAGGTAGG+GGG | 0.669065 | 1_2:-62996810 | None:intergenic |
| AATATATGTCATCACTAACG+AGG | 0.731941 | 1_2:+62997010 | Msa0083840:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATAAAAACAAAACATA+TGG | + | chr1_2:62996378-62996397 | Msa0083840:intron | 10.0% |
| !!! | TTGGTTTTATTATTTTGAAT+TGG | + | chr1_2:62994919-62994938 | Msa0083840:five_prime_UTR | 15.0% |
| !!! | TTTTAACAAAATAGTTTTAG+AGG | + | chr1_2:62995317-62995336 | Msa0083840:intron | 15.0% |
| !! | ATTTATGTCTATTATTTATG+TGG | + | chr1_2:62995383-62995402 | Msa0083840:intron | 15.0% |
| !!! | ACTTGATTAATTTTTGTTTT+AGG | + | chr1_2:62995731-62995750 | Msa0083840:intron | 15.0% |
| !!! | CTTGATTAATTTTTGTTTTA+GGG | + | chr1_2:62995732-62995751 | Msa0083840:intron | 15.0% |
| !!! | TTGATTAATTTTTGTTTTAG+GGG | + | chr1_2:62995733-62995752 | Msa0083840:intron | 15.0% |
| !!! | TTTTTGTATATGCTATAATT+TGG | - | chr1_2:62996033-62996052 | None:intergenic | 15.0% |
| !! | TTCAGAAACTACAAATAAAA+AGG | - | chr1_2:62994788-62994807 | None:intergenic | 20.0% |
| !! | TTATTTGTAGTTTCTGAATT+AGG | + | chr1_2:62994790-62994809 | Msa0083840:five_prime_UTR | 20.0% |
| !! | TATTTGTAGTTTCTGAATTA+GGG | + | chr1_2:62994791-62994810 | Msa0083840:five_prime_UTR | 20.0% |
| !! | TTCATAACTTTCTTATGAAA+TGG | + | chr1_2:62995592-62995611 | Msa0083840:intron | 20.0% |
| !! | AATGATATAATTATCCAATG+AGG | - | chr1_2:62995637-62995656 | None:intergenic | 20.0% |
| !! | TCATTGGATAATTATATCAT+TGG | + | chr1_2:62995636-62995655 | Msa0083840:intron | 20.0% |
| !! | TCATTGGATATAATATATCA+TGG | + | chr1_2:62995652-62995671 | Msa0083840:intron | 20.0% |
| !! | AACTATATGATTGTTTATGA+TGG | + | chr1_2:62995798-62995817 | Msa0083840:intron | 20.0% |
| !!! | TTTTCATAAGTTTTCCAATA+TGG | + | chr1_2:62995998-62996017 | Msa0083840:intron | 20.0% |
| !!! | TTTGGTTTTATATACCATAT+TGG | - | chr1_2:62996015-62996034 | None:intergenic | 20.0% |
| !!! | TTGTGTATTTGATAATATCA+TGG | - | chr1_2:62996336-62996355 | None:intergenic | 20.0% |
| !!! | ATTTTACTGGAGAATTTTTT+TGG | + | chr1_2:62996572-62996591 | Msa0083840:CDS | 20.0% |
| !! | GATTTCATTATTCTTGTAAA+TGG | - | chr1_2:62996786-62996805 | None:intergenic | 20.0% |
| !! | ATATTAAATGTCTTAATCTG+AGG | + | chr1_2:62997066-62997085 | Msa0083840:CDS | 20.0% |
| !!! | ACTTTTAGTAGTTTATTTCT+TGG | + | chr1_2:62997145-62997164 | Msa0083840:CDS | 20.0% |
| !! | TTCTACATTAGTCAAATTAT+TGG | - | chr1_2:62997575-62997594 | None:intergenic | 20.0% |
| !!! | AATGTAGAAAAAACTTTTGA+TGG | + | chr1_2:62997586-62997605 | Msa0083840:three_prime_UTR | 20.0% |
| !!! | CAACACATTTAATTTTGATA+CGG | - | chr1_2:62997635-62997654 | None:intergenic | 20.0% |
| !!! | ATTAATAGTTTGAGTTCTTT+TGG | + | chr1_2:62997658-62997677 | Msa0083840:three_prime_UTR | 20.0% |
| ! | ACTACAAATAAAAAGGAACA+AGG | - | chr1_2:62994781-62994800 | None:intergenic | 25.0% |
| ! | AAAATGCAATATGCATTTGA+AGG | + | chr1_2:62995511-62995530 | Msa0083840:intron | 25.0% |
| ! | AAATGCAATATGCATTTGAA+GGG | + | chr1_2:62995512-62995531 | Msa0083840:intron | 25.0% |
| ! | ATAGTATCTCAAAACAATGA+AGG | + | chr1_2:62996182-62996201 | Msa0083840:intron | 25.0% |
| ! | TAGTATCTCAAAACAATGAA+GGG | + | chr1_2:62996183-62996202 | Msa0083840:intron | 25.0% |
| ! | AATAACATGAATAACCTACA+AGG | - | chr1_2:62996270-62996289 | None:intergenic | 25.0% |
| ! | TGATAATATCATGGAAAATG+AGG | - | chr1_2:62996327-62996346 | None:intergenic | 25.0% |
| !! | GGACTTTTCATAAATTTTAC+TGG | + | chr1_2:62996559-62996578 | Msa0083840:CDS | 25.0% |
| ! | GAATAATAACTCTTAGCATA+AGG | - | chr1_2:62996630-62996649 | None:intergenic | 25.0% |
| !!! | AAGTGATAAACTTTTTGGTT+GGG | - | chr1_2:62996666-62996685 | None:intergenic | 25.0% |
| ! | AACAATAGAAAATAGAGAGT+GGG | + | chr1_2:62996959-62996978 | Msa0083840:CDS | 25.0% |
| !! | GCATTATTTTCTATTTCGTT+TGG | + | chr1_2:62997245-62997264 | Msa0083840:CDS | 25.0% |
| ! | AATATGTAGCGTTTATTCAA+GGG | + | chr1_2:62997355-62997374 | Msa0083840:CDS | 25.0% |
| !!! | TTTTAGGCATCTAAAATATG+AGG | + | chr1_2:62997463-62997482 | Msa0083840:CDS | 25.0% |
| ! | CAAATTATTGGCAAACAAAA+AGG | - | chr1_2:62997563-62997582 | None:intergenic | 25.0% |
| ! | CGTATCAAAATTAAATGTGT+TGG | + | chr1_2:62997633-62997652 | Msa0083840:three_prime_UTR | 25.0% |
| AACTATGCAAATCAGTTCAA+TGG | + | chr1_2:62994861-62994880 | Msa0083840:five_prime_UTR | 30.0% | |
| AAGATGAAGAATTTCCGAAT+CGG | + | chr1_2:62994996-62995015 | Msa0083840:exon | 30.0% | |
| TAAAACTCCTGAAAGATTTG+AGG | + | chr1_2:62995093-62995112 | Msa0083840:CDS | 30.0% | |
| AAAACTCCTGAAAGATTTGA+GGG | + | chr1_2:62995094-62995113 | Msa0083840:CDS | 30.0% | |
| GGAAGAAGAATTGATATAAG+AGG | + | chr1_2:62995119-62995138 | Msa0083840:CDS | 30.0% | |
| AGGACATACAGATTTAATTC+TGG | + | chr1_2:62995172-62995191 | Msa0083840:CDS | 30.0% | |
| GGACATACAGATTTAATTCT+GGG | + | chr1_2:62995173-62995192 | Msa0083840:CDS | 30.0% | |
| ! | TTCAAAATTTTCCTTCCAAC+TGG | + | chr1_2:62995197-62995216 | Msa0083840:CDS | 30.0% |
| AAAAGTAGTAACTAACCAGT+TGG | - | chr1_2:62995215-62995234 | None:intergenic | 30.0% | |
| TAACATGATGAACAACTACA+TGG | - | chr1_2:62995418-62995437 | None:intergenic | 30.0% | |
| CTTATTCTACTAAGTGTGAT+CGG | - | chr1_2:62995711-62995730 | None:intergenic | 30.0% | |
| TAGAAGTTGAATCGTACTTT+AGG | + | chr1_2:62995862-62995881 | Msa0083840:intron | 30.0% | |
| TGAAATAAGTCAATCCAAAC+AGG | - | chr1_2:62995889-62995908 | None:intergenic | 30.0% | |
| AATAACCTACAAGGTGATAA+GGG | - | chr1_2:62996261-62996280 | None:intergenic | 30.0% | |
| ! | ACTATCCTTGTTGTGATAAA+AGG | + | chr1_2:62996417-62996436 | Msa0083840:intron | 30.0% |
| ! | CTATCCTTGTTGTGATAAAA+GGG | + | chr1_2:62996418-62996437 | Msa0083840:intron | 30.0% |
| ! | TTTTCCCTTTTATCACAACA+AGG | - | chr1_2:62996425-62996444 | None:intergenic | 30.0% |
| TGTTGTGATAAAAGGGAAAA+AGG | + | chr1_2:62996425-62996444 | Msa0083840:intron | 30.0% | |
| GTGATATCAAACATATCCAA+AGG | - | chr1_2:62996480-62996499 | None:intergenic | 30.0% | |
| ATGAAGAAGTAATGGTTCTT+GGG | - | chr1_2:62996601-62996620 | None:intergenic | 30.0% | |
| TATGAAGAAGTAATGGTTCT+TGG | - | chr1_2:62996602-62996621 | None:intergenic | 30.0% | |
| !! | CAAGTGATAAACTTTTTGGT+TGG | - | chr1_2:62996667-62996686 | None:intergenic | 30.0% |
| CAACCAAAAAGTTTATCACT+TGG | + | chr1_2:62996665-62996684 | Msa0083840:CDS | 30.0% | |
| ! | TCTCCAAGTGATAAACTTTT+TGG | - | chr1_2:62996671-62996690 | None:intergenic | 30.0% |
| AATATGTAACATAGCTAGAG+CGG | - | chr1_2:62996710-62996729 | None:intergenic | 30.0% | |
| AGCTATGTTACATATTCTAG+CGG | + | chr1_2:62996714-62996733 | Msa0083840:CDS | 30.0% | |
| CAACAATAGAAAATAGAGAG+TGG | + | chr1_2:62996958-62996977 | Msa0083840:CDS | 30.0% | |
| AATATATGTCATCACTAACG+AGG | + | chr1_2:62997010-62997029 | Msa0083840:CDS | 30.0% | |
| ATCACAAATAACCAACCTAA+GGG | - | chr1_2:62997120-62997139 | None:intergenic | 30.0% | |
| TTTCAGTCTGACATAATTCA+TGG | - | chr1_2:62997210-62997229 | None:intergenic | 30.0% | |
| TTATGTCAGACTGAAAACTT+TGG | + | chr1_2:62997214-62997233 | Msa0083840:CDS | 30.0% | |
| TATGTCAGACTGAAAACTTT+GGG | + | chr1_2:62997215-62997234 | Msa0083840:CDS | 30.0% | |
| CAATATGTAGCGTTTATTCA+AGG | + | chr1_2:62997354-62997373 | Msa0083840:CDS | 30.0% | |
| GATGATATTCAAGAAGAGAT+GGG | - | chr1_2:62997412-62997431 | None:intergenic | 30.0% | |
| AGAAATAAATCGAGTGAAGG+AGG | - | chr1_2:62994900-62994919 | None:intergenic | 35.0% | |
| CCAAGAAATAAATCGAGTGA+AGG | - | chr1_2:62994903-62994922 | None:intergenic | 35.0% | |
| ! | CCTTCACTCGATTTATTTCT+TGG | + | chr1_2:62994900-62994919 | Msa0083840:five_prime_UTR | 35.0% |
| TGAACAAATGTGAAGTGCAA+TGG | - | chr1_2:62995239-62995258 | None:intergenic | 35.0% | |
| !! | GCTATTTCTATATGCCTCAT+TGG | + | chr1_2:62995620-62995639 | Msa0083840:intron | 35.0% |
| ! | TTATGATGGAAAAGCACACA+AGG | + | chr1_2:62995812-62995831 | Msa0083840:intron | 35.0% |
| CTTATCTACTAACGTAAGCT+TGG | + | chr1_2:62995910-62995929 | Msa0083840:intron | 35.0% | |
| ! | TGAAGACATTTAGGGTTGTT+TGG | - | chr1_2:62996139-62996158 | None:intergenic | 35.0% |
| GATCTACGTGAAGACATTTA+GGG | - | chr1_2:62996147-62996166 | None:intergenic | 35.0% | |
| AGATCTACGTGAAGACATTT+AGG | - | chr1_2:62996148-62996167 | None:intergenic | 35.0% | |
| AATGTCTTCACGTAGATCTT+TGG | + | chr1_2:62996149-62996168 | Msa0083840:intron | 35.0% | |
| TAAAACCCTTATCACCTTGT+AGG | + | chr1_2:62996253-62996272 | Msa0083840:intron | 35.0% | |
| GAATAACCTACAAGGTGATA+AGG | - | chr1_2:62996262-62996281 | None:intergenic | 35.0% | |
| AAAAGGGAAAAAGGTGAAGA+AGG | + | chr1_2:62996434-62996453 | Msa0083840:intron | 35.0% | |
| AGAGAACTATGAACTTCCTT+TGG | + | chr1_2:62996461-62996480 | Msa0083840:CDS | 35.0% | |
| GGCGATATATGAAGAAGTAA+TGG | - | chr1_2:62996609-62996628 | None:intergenic | 35.0% | |
| TTGGTTGGGTATGCTAATAA+AGG | - | chr1_2:62996652-62996671 | None:intergenic | 35.0% | |
| AGTTTATCACTTGGAGATGA+TGG | + | chr1_2:62996674-62996693 | Msa0083840:CDS | 35.0% | |
| TAAAGAAGAGTTACCTTCAG+AGG | - | chr1_2:62996820-62996839 | None:intergenic | 35.0% | |
| GTCTATCAATCTCGAAATTG+TGG | + | chr1_2:62996921-62996940 | Msa0083840:CDS | 35.0% | |
| ATCAATCTCGAAATTGTGGA+TGG | + | chr1_2:62996925-62996944 | Msa0083840:CDS | 35.0% | |
| TCAATCTCGAAATTGTGGAT+GGG | + | chr1_2:62996926-62996945 | Msa0083840:CDS | 35.0% | |
| CATCACAAATAACCAACCTA+AGG | - | chr1_2:62997121-62997140 | None:intergenic | 35.0% | |
| GTACACATTTGCACTTTCAT+TGG | - | chr1_2:62997171-62997190 | None:intergenic | 35.0% | |
| AGCAACTCTGTGTATTACAT+TGG | + | chr1_2:62997320-62997339 | Msa0083840:CDS | 35.0% | |
| GGATGATATTCAAGAAGAGA+TGG | - | chr1_2:62997413-62997432 | None:intergenic | 35.0% | |
| GAATATCATCCTACAACTGT+TGG | + | chr1_2:62997422-62997441 | Msa0083840:CDS | 35.0% | |
| TAGATGCCTAAAACACCAAT+CGG | - | chr1_2:62997456-62997475 | None:intergenic | 35.0% | |
| ATTGTACATTGCGTTACTCT+TGG | + | chr1_2:62997516-62997535 | Msa0083840:three_prime_UTR | 35.0% | |
| AATCGAGTGAAGGAGGATTA+AGG | - | chr1_2:62994893-62994912 | None:intergenic | 40.0% | |
| CGATAAACTAGGGTTTCTCA+AGG | + | chr1_2:62995022-62995041 | Msa0083840:CDS | 40.0% | |
| AGAGTGAAGAAGCTGTTGAA+AGG | + | chr1_2:62995152-62995171 | Msa0083840:CDS | 40.0% | |
| GTAGTAACTAACCAGTTGGA+AGG | - | chr1_2:62995211-62995230 | None:intergenic | 40.0% | |
| !! | ATCGTACTTTAGGACCTGTT+TGG | + | chr1_2:62995872-62995891 | Msa0083840:intron | 40.0% |
| ! | GACGACCTATTTCAGTTTTC+CGG | + | chr1_2:62996519-62996538 | Msa0083840:CDS | 40.0% |
| AAGAGTTACCTTCAGAGGTA+GGG | - | chr1_2:62996815-62996834 | None:intergenic | 40.0% | |
| ! | GCATTTGCTAAAAGCAAGCA+AGG | - | chr1_2:62996853-62996872 | None:intergenic | 40.0% |
| ! | TGCTTTTAGCAAATGCTCTG+AGG | + | chr1_2:62996857-62996876 | Msa0083840:CDS | 40.0% |
| ! | CTCTGAGGAATTTGTCTTTG+TGG | + | chr1_2:62996872-62996891 | Msa0083840:CDS | 40.0% |
| GAACTAGGAATTCCTATGAC+GGG | - | chr1_2:62997098-62997117 | None:intergenic | 40.0% | |
| GGAACTAGGAATTCCTATGA+CGG | - | chr1_2:62997099-62997118 | None:intergenic | 40.0% | |
| TAGGAATTCCTAGTTCCCTT+AGG | + | chr1_2:62997102-62997121 | Msa0083840:CDS | 40.0% | |
| AATTCCTAGTTCCCTTAGGT+TGG | + | chr1_2:62997106-62997125 | Msa0083840:CDS | 40.0% | |
| ATAACCAACCTAAGGGAACT+AGG | - | chr1_2:62997113-62997132 | None:intergenic | 40.0% | |
| ATCATCCTACAACTGTTGGT+TGG | + | chr1_2:62997426-62997445 | Msa0083840:CDS | 40.0% | |
| ! | CTAGTTTATCGTCGCCGATT+CGG | - | chr1_2:62995013-62995032 | None:intergenic | 45.0% |
| GAATCGGCGACGATAAACTA+GGG | + | chr1_2:62995012-62995031 | Msa0083840:CDS | 45.0% | |
| TTCCAGCCCTCAAATCTTTC+AGG | - | chr1_2:62995103-62995122 | None:intergenic | 45.0% | |
| ACACGCCGGAAAACTGAAAT+AGG | - | chr1_2:62996527-62996546 | None:intergenic | 45.0% | |
| AGAGTTACCTTCAGAGGTAG+GGG | - | chr1_2:62996814-62996833 | None:intergenic | 45.0% | |
| GAAGAGTTACCTTCAGAGGT+AGG | - | chr1_2:62996816-62996835 | None:intergenic | 45.0% | |
| !! | AGAGTGGGTTGTTGACTTTG+TGG | + | chr1_2:62996974-62996993 | Msa0083840:CDS | 45.0% |
| ATCACTAACGAGGCGAAGAT+AGG | + | chr1_2:62997020-62997039 | Msa0083840:CDS | 45.0% | |
| TCACTAACGAGGCGAAGATA+GGG | + | chr1_2:62997021-62997040 | Msa0083840:CDS | 45.0% | |
| ATTGAGCAACCCGAACAAGT+GGG | + | chr1_2:62997289-62997308 | Msa0083840:CDS | 45.0% | |
| TCTCATATCCCCACTTGTTC+GGG | - | chr1_2:62997301-62997320 | None:intergenic | 45.0% | |
| CTCTCATATCCCCACTTGTT+CGG | - | chr1_2:62997302-62997321 | None:intergenic | 45.0% | |
| CACTCGAATCATCCCTTCCA+CGG | + | chr1_2:62994828-62994847 | Msa0083840:five_prime_UTR | 50.0% | |
| CGAATCGGCGACGATAAACT+AGG | + | chr1_2:62995011-62995030 | Msa0083840:CDS | 50.0% | |
| CTCCTGAAAGATTTGAGGGC+TGG | + | chr1_2:62995098-62995117 | Msa0083840:CDS | 50.0% | |
| TTTCCGGCGTGTGCAAGAAT+TGG | + | chr1_2:62996535-62996554 | Msa0083840:CDS | 50.0% | |
| TCAATTCCCCCTACCTCTGA+AGG | + | chr1_2:62996804-62996823 | Msa0083840:CDS | 50.0% | |
| GAGTTACCTTCAGAGGTAGG+GGG | - | chr1_2:62996813-62996832 | None:intergenic | 50.0% | |
| CACTAACGAGGCGAAGATAG+GGG | + | chr1_2:62997022-62997041 | Msa0083840:CDS | 50.0% | |
| CATTGAGCAACCCGAACAAG+TGG | + | chr1_2:62997288-62997307 | Msa0083840:CDS | 50.0% | |
| TTGAGCAACCCGAACAAGTG+GGG | + | chr1_2:62997290-62997309 | Msa0083840:CDS | 50.0% | |
| GCGTTCCAACCAACAGTTGT+AGG | - | chr1_2:62997434-62997453 | None:intergenic | 50.0% | |
| !! | GGAACGCCGATTGGTGTTTT+AGG | + | chr1_2:62997447-62997466 | Msa0083840:CDS | 50.0% |
| TTGAACGAGCAGCCGTGGAA+GGG | - | chr1_2:62994843-62994862 | None:intergenic | 55.0% | |
| CATAGTTGAACGAGCAGCCG+TGG | - | chr1_2:62994848-62994867 | None:intergenic | 55.0% | |
| CCTCCAATTCTTGCACACGC+CGG | - | chr1_2:62996541-62996560 | None:intergenic | 55.0% | |
| !! | CTGAGGAGTACTCCCGTCAT+AGG | + | chr1_2:62997083-62997102 | Msa0083840:CDS | 55.0% |
| ! | CTGTTGGTTGGAACGCCGAT+TGG | + | chr1_2:62997438-62997457 | Msa0083840:CDS | 55.0% |
| GTTGAACGAGCAGCCGTGGA+AGG | - | chr1_2:62994844-62994863 | None:intergenic | 60.0% | |
| CCGGCGTGTGCAAGAATTGG+AGG | + | chr1_2:62996538-62996557 | Msa0083840:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 62994722 | 62997703 | 62994722 | ID=Msa0083840;Name=Msa0083840 |
| chr1_2 | mRNA | 62994722 | 62997703 | 62994722 | ID=Msa0083840-mRNA-1;Parent=Msa0083840;Name=Msa0083840-mRNA-1;_AED=0.03;_eAED=0.03;_QI=277|1|1|1|1|1|2|195|429 |
| chr1_2 | exon | 62994722 | 62995218 | 62994722 | ID=Msa0083840-mRNA-1:exon:21054;Parent=Msa0083840-mRNA-1 |
| chr1_2 | exon | 62996439 | 62997703 | 62996439 | ID=Msa0083840-mRNA-1:exon:21055;Parent=Msa0083840-mRNA-1 |
| chr1_2 | five_prime_UTR | 62994722 | 62994998 | 62994722 | ID=Msa0083840-mRNA-1:five_prime_utr;Parent=Msa0083840-mRNA-1 |
| chr1_2 | CDS | 62994999 | 62995218 | 62994999 | ID=Msa0083840-mRNA-1:cds;Parent=Msa0083840-mRNA-1 |
| chr1_2 | CDS | 62996439 | 62997508 | 62996439 | ID=Msa0083840-mRNA-1:cds;Parent=Msa0083840-mRNA-1 |
| chr1_2 | three_prime_UTR | 62997509 | 62997703 | 62997509 | ID=Msa0083840-mRNA-1:three_prime_utr;Parent=Msa0083840-mRNA-1 |
| Gene Sequence |
| Protein sequence |
