AlfalfaGEDB | Msa0085270

M. sativa cultivar zhongmu-4 / Msa0085270

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0085270	KEH43546.1	87.302	252	14	1	1	234	544	795	1.14e-147	441

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0085270	sp\|O22938\|PXC3_ARATH	36.782	261	130	7	1	230	627	883	7.95e-39	145

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0085270	A0A072VPG1	87.302	252	14	1	1	234	544	795	5.44e-148	441

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0085270	MtrunA17_Chr1g0200461	87.649	251	14	1	1	234	679	929	3.79e-150	441
Msa0085270	MtrunA17_Chr1g0200471	79.608	255	31	2	1	234	667	921	3.69e-137	407
Msa0085270	MtrunA17_Chr7g0257621	35.632	261	133	7	1	230	626	882	8.76e-43	154
Msa0085270	MtrunA17_Chr2g0280191	32.759	232	133	3	26	234	735	966	6.03e-36	134
Msa0085270	MtrunA17_Chr1g0183231	32.819	259	146	6	3	234	723	980	3.08e-35	132
Msa0085270	MtrunA17_Chr3g0140871	32.189	233	133	3	26	233	730	962	2.31e-34	130
Msa0085270	MtrunA17_Chr1g0169841	34.599	237	126	6	25	233	854	1089	3.21e-34	129
Msa0085270	MtrunA17_Chr2g0312651	34.351	262	142	7	1	234	827	1086	3.41e-34	129
Msa0085270	MtrunA17_Chr1g0189871	34.914	232	123	4	27	230	845	1076	7.54e-34	128
Msa0085270	MtrunA17_Chr7g0237821	32.468	231	130	4	25	230	841	1070	2.36e-33	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0085270	AT1G34420.1	52.140	257	96	4	1	230	707	963	6.26e-76	247
Msa0085270	AT2G41820.1	36.782	261	130	7	1	230	627	883	8.08e-40	145
Msa0085270	AT1G17230.2	36.226	265	132	7	1	234	818	1076	2.33e-37	139
Msa0085270	AT1G17230.1	36.226	265	132	7	1	234	818	1076	2.45e-37	139
Msa0085270	AT1G78530.1	33.621	232	123	6	27	230	116	344	7.20e-35	127
Msa0085270	AT3G24240.1	33.766	231	127	3	25	230	834	1063	8.05e-35	131
Msa0085270	AT5G61480.1	33.333	264	146	6	1	234	738	1001	8.59e-34	129
Msa0085270	AT1G09970.1	30.638	235	137	3	26	234	721	955	2.13e-33	127
Msa0085270	AT2G33170.2	33.585	265	144	8	1	233	838	1102	2.78e-33	127
Msa0085270	AT2G33170.1	33.585	265	144	8	1	233	838	1102	2.78e-33	127

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Find 46 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAAATGCTTGAAATTC+TGG	0.159792	1_2:-65249126	Msa0085270:CDS
AAAGAGAATGTGTATAGCTT+TGG	0.344003	1_2:-65249287	Msa0085270:CDS
AAGAGAATGTGTATAGCTTT+GGG	0.439434	1_2:-65249286	Msa0085270:CDS
AAGGAGAAGTATTGGACCAG+AGG	0.647975	1_2:+65249862	None:intergenic
AATTTATCAAGACTACTCAC+TGG	0.574924	1_2:+65250109	None:intergenic
AGACTCCCAGTACTCTTTGA+AGG	0.472278	1_2:+65249758	None:intergenic
AGAGAATGTGTATAGCTTTG+GGG	0.750219	1_2:-65249285	Msa0085270:CDS
AGCAGATATAGTATAGCTGT+TGG	0.588684	1_2:-65249920	Msa0085270:CDS
AGGGAGCTGAATTGGTAAAG+TGG	0.550487	1_2:-65249220	Msa0085270:CDS
AGTGACTAAGGGAGCTGAAT+TGG	0.399875	1_2:-65249228	Msa0085270:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTTATCAAGACTACTCAC+TGG	+	chr1_2:65249100-65249119	None:intergenic	30.0%
CATGAAAGACACTTTAGATT+GGG	-	chr1_2:65249261-65249280	Msa0085270:CDS	30.0%
TGTTCAATATCACCAATTAG+AGG	+	chr1_2:65249412-65249431	None:intergenic	30.0%
GCTTATGAATTTAGACTAAC+TGG	+	chr1_2:65249536-65249555	None:intergenic	30.0%
ATGAACACATATATGATAGG+TGG	-	chr1_2:65249708-65249727	Msa0085270:intron	30.0%
AAAGAGAATGTGTATAGCTT+TGG	-	chr1_2:65249919-65249938	Msa0085270:CDS	30.0%
AAGAGAATGTGTATAGCTTT+GGG	-	chr1_2:65249920-65249939	Msa0085270:CDS	30.0%
AAACCAAATGCTTGAAATTC+TGG	-	chr1_2:65250080-65250099	Msa0085270:CDS	30.0%
AATTTATCAAGACTACTCAC+TGG	+	chr1_2:65249100-65249119	None:intergenic	30.0%
CATGAAAGACACTTTAGATT+GGG	-	chr1_2:65249261-65249280	Msa0085270:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	65249040	65250188	65249040	ID=Msa0085270;Name=Msa0085270
chr1_2	mRNA	65249040	65250188	65249040	ID=Msa0085270-mRNA-1;Parent=Msa0085270;Name=Msa0085270-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0\|0\|0\|1\|1\|1\|2\|0\|234
chr1_2	exon	65249771	65250188	65249771	ID=Msa0085270-mRNA-1:exon:22050;Parent=Msa0085270-mRNA-1
chr1_2	exon	65249040	65249326	65249040	ID=Msa0085270-mRNA-1:exon:22049;Parent=Msa0085270-mRNA-1
chr1_2	CDS	65249771	65250188	65249771	ID=Msa0085270-mRNA-1:cds;Parent=Msa0085270-mRNA-1
chr1_2	CDS	65249040	65249326	65249040	ID=Msa0085270-mRNA-1:cds;Parent=Msa0085270-mRNA-1

Gene Sequence

>Msa0085270

ATGCCATCTGGTTCAATCTATTTCGCCAAGAAGCTGAACTGGTGCGACAAGGTATTTCCAGTGAGTAGTCTTGATAAATTTGGGAAAGAGCTAGATGCATTGGCAAAGTTGAACAATTCAAATGTGATGATTCCACTAGGCTATATTGTGTCCACTAACAATGTTTATATATTATACGAGTTTCTCTCGAATGGCTCATTCTTCGATGTGCTTCATGGTGGCATGAAAGACACTTTAGATTGGGCTAGCAGATATAGTATAGCTGTTGGTGTGGCTCAAGGCCTAGATTTTCTGCATGGTTGTGCCTCTGGTCCAATACTTCTCCTTGATCTATCAAGTAAAAGTATTATGCTGAAATCGCTTGAGGAGCCTCTAATTGGTGATATTGAACACTGCAAGGTGATTGATCCTTCAAAGAAGTATGCGTGCACAATGAAAGAGAATGTGTATAGCTTTGGGGTGATTCTACTGGAATTGCTGACTGGAAAACCTGCAGTGACTAAGGGAGCTGAATTGGTAAAGTGGGTTTTGCGTAATTCAAGAAATCAAGATTACATTCTTGATTTGAATGTCAGCAAAACATCACAGTCTGTTAGAAACCAAATGCTTGAAATTCTGGAGATTGCTCTTGTTTGTGTAAGCACATCCCCTGATGCAAGACCAAAGATGAAGACTGTGCTAAGAATGTTGCTGAATGCCAGATAA

Protein sequence

>Msa0085270

MPSGSIYFAKKLNWCDKVFPVSSLDKFGKELDALAKLNNSNVMIPLGYIVSTNNVYILYEFLSNGSFFDVLHGGMKDTLDWASRYSIAVGVAQGLDFLHGCASGPILLLDLSSKSIMLKSLEEPLIGDIEHCKVIDPSKKYACTMKENVYSFGVILLELLTGKPAVTKGAELVKWVLRNSRNQDYILDLNVSKTSQSVRNQMLEILEIALVCVSTSPDARPKMKTVLRMLLNAR*