AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0087230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087230 MtrunA17_Chr1g0203051 45.367 626 326 4 6 624 5 621 0.0 573
Msa0087230 MtrunA17_Chr2g0287651 35.694 720 439 7 73 784 66 769 1.26e-151 466
Msa0087230 MtrunA17_Chr2g0308841 77.434 226 51 0 411 636 1 226 1.60e-127 382
Msa0087230 MtrunA17_Chr5g0448761 31.588 592 391 8 84 662 62 652 6.91e-102 332
Msa0087230 MtrunA17_Chr1g0202341 31.383 564 372 8 83 637 52 609 6.22e-90 298
Msa0087230 MtrunA17_Chr2g0324011 29.875 559 377 8 88 637 16 568 1.23e-83 278
Msa0087230 MtrunA17_Chr1g0198761 28.532 722 456 21 88 775 53 748 6.99e-75 259
Msa0087230 MtrunA17_Chr5g0441581 27.027 666 445 16 88 736 38 679 2.15e-74 256
Msa0087230 MtrunA17_Chr2g0296781 27.586 667 440 17 97 745 12 653 4.73e-74 256
Msa0087230 MtrunA17_Chr8g0363841 28.428 598 397 9 55 640 2 580 1.03e-72 251
Msa0087230 MtrunA17_Chr1g0203721 28.622 566 377 10 88 637 69 623 4.19e-70 248
Msa0087230 MtrunA17_Chr3g0118611 29.652 489 330 6 238 716 96 580 2.42e-69 244
Msa0087230 MtrunA17_Chr6g0477261 28.957 556 359 13 208 745 5 542 3.51e-69 241
Msa0087230 MtrunA17_Chr5g0448511 29.750 400 273 4 247 640 3 400 5.02e-68 235
Msa0087230 MtrunA17_Chr2g0287061 29.125 594 361 16 59 640 159 704 6.68e-67 237
Msa0087230 MtrunA17_Chr1g0207551 28.648 562 379 11 87 637 39 589 9.32e-67 237
Msa0087230 MtrunA17_Chr5g0440451 27.288 601 391 11 88 662 49 629 3.08e-66 238
Msa0087230 MtrunA17_Chr6g0458391 25.359 627 416 14 33 637 169 765 2.25e-65 233
Msa0087230 MtrunA17_Chr6g0458391 35.714 126 70 4 87 208 96 214 2.02e-11 67.8
Msa0087230 MtrunA17_Chr3g0082121 26.557 610 413 12 39 637 5 590 8.37e-64 227
Msa0087230 MtrunA17_Chr1g0196541 27.289 557 377 10 97 637 12 556 7.10e-60 216
Msa0087230 MtrunA17_Chr3g0080351 27.671 571 365 12 87 637 81 623 8.89e-60 218
Msa0087230 MtrunA17_Chr5g0431001 30.166 421 265 8 88 486 67 480 8.78e-59 208
Msa0087230 MtrunA17_Chr2g0276841 27.634 579 369 17 88 639 76 631 1.29e-56 206
Msa0087230 MtrunA17_Chr2g0291591 26.772 508 349 10 83 579 65 560 2.53e-55 200
Msa0087230 MtrunA17_Chr2g0291581 25.445 562 386 11 87 634 76 618 4.02e-55 202
Msa0087230 MtrunA17_Chr2g0290391 26.795 571 377 12 88 634 57 610 1.43e-53 197
Msa0087230 MtrunA17_Chr2g0329031 23.555 692 480 15 87 752 19 687 1.39e-51 192
Msa0087230 MtrunA17_Chr2g0294841 26.362 569 379 15 88 632 51 603 3.07e-46 177
Msa0087230 MtrunA17_Chr1g0164591 28.239 301 204 2 174 469 14 307 1.54e-39 149
Msa0087230 MtrunA17_Chr1g0171741 40.373 161 94 2 53 212 103 262 7.48e-31 122
Msa0087230 MtrunA17_Chr8g0389811 42.520 127 73 0 67 193 69 195 3.14e-29 115
Msa0087230 MtrunA17_Chr7g0258861 27.491 291 197 7 88 370 16 300 8.28e-29 117
Msa0087230 MtrunA17_Chr2g0331371 42.038 157 89 1 32 186 3 159 7.91e-28 112
Msa0087230 MtrunA17_Chr6g0473421 46.316 95 51 0 64 158 37 131 2.08e-23 97.4
Msa0087230 MtrunA17_Chr1g0172931 36.508 126 80 0 39 164 33 158 5.62e-19 84.7
Msa0087230 MtrunA17_Chr8g0340801 34.579 107 70 0 193 299 1 107 1.80e-18 81.6
Msa0087230 MtrunA17_Chr1g0149131 30.435 115 65 3 361 465 1 110 1.64e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0087230 AT4G38180.1 30.819 623 409 10 38 640 17 637 1.80e-96 318
Msa0087230 AT3G06250.2 29.464 560 336 13 87 637 195 704 3.38e-73 254
Msa0087230 AT3G06250.1 29.464 560 336 13 87 637 195 704 3.38e-73 254
Msa0087230 AT3G06250.3 29.464 560 336 13 87 637 195 704 3.38e-73 254
Msa0087230 AT4G15090.1 27.379 683 445 18 87 746 56 710 1.25e-71 251
Msa0087230 AT4G15090.5 27.379 683 445 18 87 746 56 710 1.25e-71 251
Msa0087230 AT4G15090.3 27.379 683 445 18 87 746 56 710 1.25e-71 251
Msa0087230 AT4G15090.2 27.379 683 445 18 87 746 56 710 1.25e-71 251
Msa0087230 AT4G15090.4 27.379 683 445 18 87 746 56 710 1.25e-71 251
Msa0087230 AT5G18960.2 30.233 559 336 15 87 637 216 728 4.78e-69 243
Msa0087230 AT5G18960.1 30.233 559 336 15 87 637 216 728 4.78e-69 243
Msa0087230 AT2G27110.2 27.170 622 401 12 24 640 8 582 5.06e-68 242
Msa0087230 AT2G27110.1 27.170 622 401 12 24 640 8 582 5.06e-68 242
Msa0087230 AT2G27110.3 30.303 396 268 3 250 640 45 437 1.41e-65 232
Msa0087230 AT1G80010.1 28.966 580 385 13 87 647 100 671 9.56e-62 222
Msa0087230 AT3G22170.2 27.778 576 359 14 87 637 75 618 1.35e-60 220
Msa0087230 AT3G22170.1 27.778 576 359 14 87 637 75 618 1.35e-60 220
Msa0087230 AT1G52520.1 26.624 631 411 17 87 685 90 700 3.01e-59 214
Msa0087230 AT1G76320.3 27.224 562 381 11 87 638 2 545 5.13e-58 210
Msa0087230 AT1G76320.2 27.224 562 381 11 87 638 2 545 6.28e-58 211
Msa0087230 AT1G76320.1 27.224 562 381 11 87 638 2 545 7.26e-58 211
Msa0087230 AT1G76320.4 27.224 562 381 11 87 638 2 545 7.26e-58 211
Msa0087230 AT1G10240.2 28.319 565 377 15 88 636 54 606 1.62e-57 209
Msa0087230 AT1G10240.1 28.319 565 377 15 88 636 54 606 1.62e-57 209
Msa0087230 AT4G38170.1 29.474 380 252 6 279 647 38 412 1.13e-55 201
Msa0087230 AT2G32250.8 25.812 554 359 12 87 634 2 509 1.45e-52 196
Msa0087230 AT2G32250.6 25.812 554 359 12 87 634 43 550 1.78e-52 196
Msa0087230 AT2G32250.1 25.812 554 359 12 87 634 43 550 1.78e-52 196
Msa0087230 AT2G32250.3 25.812 554 359 12 87 634 43 550 1.78e-52 196
Msa0087230 AT2G32250.7 25.812 554 359 12 87 634 2 509 1.81e-52 196
Msa0087230 AT2G32250.5 25.812 554 359 12 87 634 43 550 1.91e-52 196
Msa0087230 AT2G32250.2 25.812 554 359 12 87 634 43 550 1.92e-52 196
Msa0087230 AT2G32250.4 25.812 554 359 12 87 634 43 550 1.92e-52 196
Msa0087230 AT5G28530.3 26.842 570 378 16 88 634 61 614 8.09e-50 187
Msa0087230 AT5G28530.2 26.842 570 378 16 88 634 61 614 8.09e-50 187
Msa0087230 AT5G28530.1 26.923 572 379 16 88 634 61 618 2.84e-49 185
Msa0087230 AT4G19990.7 24.471 662 433 15 87 722 40 660 1.36e-46 179
Msa0087230 AT4G19990.3 24.471 662 433 15 87 722 26 646 1.54e-46 178
Msa0087230 AT4G19990.4 24.471 662 433 15 87 722 26 646 1.54e-46 178
Msa0087230 AT4G19990.5 24.471 662 433 15 87 722 26 646 1.59e-46 178
Msa0087230 AT4G19990.6 24.471 662 433 15 87 722 26 646 2.22e-46 178
Msa0087230 AT4G19990.2 24.471 662 433 15 87 722 26 646 2.22e-46 178
Msa0087230 AT4G19990.1 24.204 628 410 14 120 722 3 589 2.25e-43 168
Msa0087230 AT5G28530.5 24.552 558 343 17 88 634 61 551 2.36e-32 134
Msa0087230 AT5G28530.4 24.643 560 344 17 88 634 61 555 6.70e-32 132

Find 166 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGCTTTGGCAGATGCTTC+TGG 0.082781 1_2:+68133562 Msa0087230:CDS
CAAATTGCTGCAAGGTATTC+TGG 0.243533 1_2:+68134855 Msa0087230:CDS
CATCACAGGAAGACCGTAAT+TGG 0.250209 1_2:-68134445 None:intergenic
TTTGTTCATGTCAAGGTTAC+AGG 0.251328 1_2:+68133278 Msa0087230:CDS
TTAATCGGCTTAATTTAAAA+AGG 0.255566 1_2:+68134967 Msa0087230:CDS
GGGCTTTGGCAGATGCTTCT+GGG 0.266190 1_2:+68133563 Msa0087230:CDS
CTGGGCAAAACCAAACATTT+TGG 0.278132 1_2:-68134765 None:intergenic
CGTATGAACTATGAAATATT+TGG 0.287714 1_2:+68133784 Msa0087230:CDS
TCAGAGATGGTTCAAAAGTT+TGG 0.288835 1_2:+68134189 Msa0087230:CDS
CACGAGCATTTCAAAATAAC+TGG 0.290463 1_2:-68134666 None:intergenic
ATGCCAATGGATCTCTCTAT+TGG 0.291523 1_2:+68134826 Msa0087230:CDS
GATCACAATCATGTTATGTT+AGG 0.300544 1_2:+68133433 Msa0087230:CDS
CCGCAACAACTTTACACATT+TGG 0.310861 1_2:-68134884 None:intergenic
CTGTGATGCAGACCAGTTTA+CGG 0.319178 1_2:+68134460 Msa0087230:CDS
CAGAGATGGTTCAAAAGTTT+GGG 0.327286 1_2:+68134190 Msa0087230:CDS
TTCAGATAAGACATAAAATC+AGG 0.354985 1_2:-68134117 None:intergenic
TGATGATCTTCCAAAATGTT+TGG 0.355568 1_2:+68134755 Msa0087230:CDS
ATAAGAGGAAGTTTCTTTGC+TGG 0.359753 1_2:+68134276 Msa0087230:CDS
TTTGATTACCAATCCAATTA+CGG 0.365424 1_2:+68134432 Msa0087230:CDS
TCCGATTATGATAAAAGATA+TGG 0.367150 1_2:+68133075 Msa0087230:CDS
AACTGAAAATCCTGACGTTC+TGG 0.369216 1_2:-68133200 None:intergenic
TTCAAATTCAGTTGAAGGCT+TGG 0.371929 1_2:+68133030 Msa0087230:CDS
CGCACCACAGGTTGTGTGCT+TGG 0.372101 1_2:+68134058 Msa0087230:CDS
GACAGTTGTACTTGTTCTTC+CGG 0.377311 1_2:-68133834 None:intergenic
TTAACAAATTTGAAGATTTG+TGG 0.386614 1_2:+68134166 Msa0087230:CDS
GGAGGCTTGAGCGGTTGTTC+TGG 0.392707 1_2:+68133746 Msa0087230:CDS
CACAACCTGTGGTGCGCTGC+AGG 0.401311 1_2:-68134051 None:intergenic
ATCTGAGCGGTGAAATTAAT+CGG 0.405130 1_2:+68134952 Msa0087230:CDS
GGAAGTTCAAATTCAGTTGA+AGG 0.407056 1_2:+68133025 Msa0087230:CDS
TGTTGGTTTGCCAGAACGTC+AGG 0.409400 1_2:+68133190 Msa0087230:CDS
TCAAGGTTACAGGGAGGATA+GGG 0.412309 1_2:+68133288 Msa0087230:CDS
GTACCAAATGTATGGGGCTT+TGG 0.414713 1_2:+68133549 Msa0087230:CDS
CTGCCAAAGCCCCATACATT+TGG 0.417656 1_2:-68133552 None:intergenic
ACACAAACTTGCAGCGTTTG+TGG 0.418146 1_2:+68135128 Msa0087230:CDS
TGCCAATGGATCTCTCTATT+GGG 0.422416 1_2:+68134827 Msa0087230:CDS
TGGGACAATACGTGCATTCT+CGG 0.427747 1_2:+68134340 Msa0087230:CDS
TTTCTCTAATGACCGTAAAC+TGG 0.429918 1_2:-68134472 None:intergenic
TGTCCGTTTGTTGTGTTCTC+AGG 0.434603 1_2:+68133853 Msa0087230:CDS
CGTAAACTGGTCTGCATCAC+AGG 0.434963 1_2:-68134459 None:intergenic
GAAATGCTCGTGTCTTAGAA+TGG 0.443162 1_2:+68134677 Msa0087230:CDS
CAGCCATACATATGTTTCTT+CGG 0.445264 1_2:-68133932 None:intergenic
GATTGAATCTTTAGCAAACT+TGG 0.446051 1_2:-68134793 None:intergenic
TTTAGCAAACTTGGACCACC+TGG 0.451664 1_2:-68134784 None:intergenic
TGCAAAATTTCGAGTCCACT+TGG 0.456820 1_2:+68133369 Msa0087230:CDS
ATAAGACCGTACCAAATGTA+TGG 0.463653 1_2:+68133541 Msa0087230:CDS
GGCTTTGGCAGATGCTTCTG+GGG 0.464116 1_2:+68133564 Msa0087230:CDS
GGAATGCTTCCACCAACATT+GGG 0.470110 1_2:+68134091 Msa0087230:CDS
CCATCAGTCGCATCAATGTT+GGG 0.475672 1_2:+68135183 Msa0087230:CDS
GATGGTTCAAAAGTTTGGGT+TGG 0.478957 1_2:+68134194 Msa0087230:CDS
AGGAATGCTTCCACCAACAT+TGG 0.480486 1_2:+68134090 Msa0087230:CDS
CCCTTTGTTCTTACACATTG+TGG 0.482784 1_2:-68135062 None:intergenic
ACCTTTGTTTGTTCATGTCA+AGG 0.486075 1_2:+68133271 Msa0087230:CDS
CAAAATACCGTGGTGATGGT+AGG 0.487602 1_2:+68134616 Msa0087230:CDS
ACCGTGGTGATGGTAGGGTT+TGG 0.493348 1_2:+68134622 Msa0087230:CDS
GCGTCTACCAGGAGTACCAC+TGG 0.495794 1_2:-68135099 None:intergenic
ATGAACAAACAAAGGTCTGC+TGG 0.497463 1_2:-68133264 None:intergenic
CGTATTGTCCCATGTGGCTA+TGG 0.499218 1_2:-68134329 None:intergenic
ATCTACGTTGCTTGGCAACC+TGG 0.500008 1_2:-68133627 None:intergenic
TCCATCAGTCGCATCAATGT+TGG 0.500041 1_2:+68135182 Msa0087230:CDS
GTGTAAAGTTGTTGCGGAGA+TGG 0.500773 1_2:+68134890 Msa0087230:CDS
TGTGTGCTTGGCATCTGTTA+AGG 0.501921 1_2:+68134070 Msa0087230:CDS
TGGGCCACCGCTTACATAAG+AGG 0.502992 1_2:+68134261 Msa0087230:CDS
TGTTGTACGCATCCTCGTCT+TGG 0.504705 1_2:-68135244 None:intergenic
AAATTCAGTTGAAGGCTTGG+AGG 0.512939 1_2:+68133033 Msa0087230:CDS
AAACAATGATCTACGTTGCT+TGG 0.514058 1_2:-68133635 None:intergenic
TTGGGTTGGAAAACAACAGT+TGG 0.516327 1_2:+68134208 Msa0087230:CDS
TTGCAGCGTTTGTGGTGTCA+TGG 0.518568 1_2:+68135136 Msa0087230:CDS
GTCAAGGTTACAGGGAGGAT+AGG 0.518923 1_2:+68133287 Msa0087230:CDS
TGAAGATTTGTGGTCAGAGA+TGG 0.520140 1_2:+68134176 Msa0087230:CDS
ATCAGGAATCCCAATGTTGG+TGG 0.524563 1_2:-68134100 None:intergenic
GCTTTGGCAGATGCTTCTGG+GGG 0.524995 1_2:+68133565 Msa0087230:CDS
CGTCTACCAGGAGTACCACT+GGG 0.526093 1_2:-68135098 None:intergenic
AAAATACCGTGGTGATGGTA+GGG 0.526246 1_2:+68134617 Msa0087230:CDS
CCACAATGTGTAAGAACAAA+GGG 0.527111 1_2:+68135062 Msa0087230:CDS
CAGGAAGACCGTAATTGGAT+TGG 0.527713 1_2:-68134440 None:intergenic
TGTGTGCGTCTGCGTCTACC+AGG 0.527784 1_2:-68135110 None:intergenic
TTCACTTACATTGTAACCCT+CGG 0.528797 1_2:-68135267 None:intergenic
CACAATCATGTTATGTTAGG+AGG 0.529443 1_2:+68133436 Msa0087230:CDS
TGTCTTAGAATGGAATCGCT+TGG 0.529768 1_2:+68134687 Msa0087230:CDS
CAAAGCAATGGAGATGTCAA+TGG 0.531347 1_2:+68133004 Msa0087230:CDS
CCCAACATTGATGCGACTGA+TGG 0.535314 1_2:-68135183 None:intergenic
AAAGCTGTATTGACGATGAT+AGG 0.536822 1_2:+68133725 Msa0087230:CDS
TAAGACCGTACCAAATGTAT+GGG 0.537974 1_2:+68133542 Msa0087230:CDS
ACACCTGAGAACACAACAAA+CGG 0.540110 1_2:-68133856 None:intergenic
GTTGTACTTGTTCTTCCGGT+AGG 0.540774 1_2:-68133830 None:intergenic
CTTCCGGTAGGTTGCATCGA+AGG 0.540939 1_2:-68133818 None:intergenic
ACACCATCGCGCTCTAACAA+AGG 0.543044 1_2:-68132839 None:intergenic
AGAAACATATGTATGGCTGT+TGG 0.549469 1_2:+68133936 Msa0087230:CDS
ATTGAGCGTAGTCGAAAAGC+TGG 0.551197 1_2:+68133517 Msa0087230:CDS
TTGTTCATGTCAAGGTTACA+GGG 0.556666 1_2:+68133279 Msa0087230:CDS
AGACCGAAGAAACATATGTA+TGG 0.556851 1_2:+68133929 Msa0087230:CDS
TGCTTCTTAGAGTTACAGAA+AGG 0.559728 1_2:+68134559 Msa0087230:CDS
GAGCGGTTGTTCTGGTGTGA+CGG 0.561057 1_2:+68133754 Msa0087230:CDS
GCTAACCCCAGTGGTACTCC+TGG 0.561368 1_2:+68135092 Msa0087230:CDS
GTTTCAAAATACCGTGGTGA+TGG 0.561793 1_2:+68134612 Msa0087230:CDS
GTCCCAATAGAGAGATCCAT+TGG 0.565136 1_2:-68134829 None:intergenic
AGAAATGGACGGACGTTCAG+AGG 0.566572 1_2:+68132808 None:intergenic
TCGTAAGGGGCAAGTGGTGA+GGG 0.567327 1_2:+68133222 Msa0087230:CDS
ACGCTCAATTTCAGCTATGT+CGG 0.568884 1_2:-68133503 None:intergenic
AAAATCAGGAATCCCAATGT+TGG 0.569033 1_2:-68134103 None:intergenic
ATCCGTGGTAGTTATGCCAA+TGG 0.569518 1_2:+68134813 Msa0087230:CDS
TTCGTAAGGGGCAAGTGGTG+AGG 0.571006 1_2:+68133221 Msa0087230:CDS
TGCAGCGTTTGTGGTGTCAT+GGG 0.575980 1_2:+68135137 Msa0087230:CDS
TAGCCTTCGATGCAACCTAC+CGG 0.576441 1_2:+68133815 Msa0087230:CDS
TTCAGTTCGTAAGGGGCAAG+TGG 0.578338 1_2:+68133216 Msa0087230:CDS
CCTTTGTCAGTTATAACAGA+TGG 0.578591 1_2:+68133991 Msa0087230:CDS
TGATATAATGAAGATTGACC+TGG 0.579924 1_2:+68133099 Msa0087230:CDS
ATTCCTTCGTCAGTTGACAT+TGG 0.580868 1_2:-68135218 None:intergenic
AGAAAGGCAAGAAATGTCCA+TGG 0.584202 1_2:+68134575 Msa0087230:CDS
TGTGTAAGAACAAAGGGCTG+TGG 0.586971 1_2:+68135068 Msa0087230:CDS
CGAGAATGCTGAGAATGTGT+TGG 0.587048 1_2:+68135037 Msa0087230:CDS
GAAAGGCAAGAAATGTCCAT+GGG 0.588349 1_2:+68134576 Msa0087230:CDS
CAAGTGGTGAGGGATCGAAA+TGG 0.589736 1_2:+68133232 Msa0087230:CDS
ACATTGATGCGACTGATGGA+AGG 0.591550 1_2:-68135179 None:intergenic
TCCACAATGTGTAAGAACAA+AGG 0.597614 1_2:+68135061 Msa0087230:CDS
CCAAATGTGTAAAGTTGTTG+CGG 0.598797 1_2:+68134884 Msa0087230:CDS
TTTGCTAAAGATTCAATCCG+TGG 0.606851 1_2:+68134798 Msa0087230:CDS
AACCGTGAAAATAAACCCCA+TGG 0.607032 1_2:-68134592 None:intergenic
TGTACGAAAAGAAGAAGATG+TGG 0.608246 1_2:+68134241 Msa0087230:CDS
CAAGGTTACAGGGAGGATAG+GGG 0.609406 1_2:+68133289 Msa0087230:CDS
AAACCAACAGTAAAACATGT+AGG 0.611187 1_2:-68133176 None:intergenic
CGCTCAGATATTCGACAACA+CGG 0.612178 1_2:-68134938 None:intergenic
GAAACTTCCTCTTATGTAAG+CGG 0.612409 1_2:-68134268 None:intergenic
TGCGACTGGTATAGTAACGC+GGG 0.613886 1_2:+68133906 Msa0087230:CDS
AAGGTTACAGGGAGGATAGG+GGG 0.615842 1_2:+68133290 Msa0087230:CDS
AAAGCCCCATACATTTGGTA+CGG 0.620279 1_2:-68133547 None:intergenic
AAGAACCACCTCTCGTTGTG+AGG 0.620891 1_2:+68134302 Msa0087230:CDS
GCTGTATTGACGATGATAGG+AGG 0.621205 1_2:+68133728 Msa0087230:CDS
ACAAATTGCGAACAAAGCAA+TGG 0.627176 1_2:+68132992 Msa0087230:CDS
TGAGGCACTCCATAGCCACA+TGG 0.629459 1_2:+68134320 Msa0087230:CDS
AATTTGTGAACCAGTTCCAG+TGG 0.630564 1_2:+68134998 Msa0087230:CDS
CCATCTGTTATAACTGACAA+AGG 0.632179 1_2:-68133991 None:intergenic
CGAAGGAATTCTCCAAGACG+AGG 0.638814 1_2:+68135232 Msa0087230:CDS
TGCTAATCAGTTTACGAAGG+CGG 0.639264 1_2:+68134500 Msa0087230:CDS
TTCACGGTTTCAAAATACCG+TGG 0.639319 1_2:+68134606 Msa0087230:CDS
ATGGAGTGCCTCACAACGAG+AGG 0.641804 1_2:-68134310 None:intergenic
GCCTCGACAAAATATCCAAG+TGG 0.642964 1_2:-68133384 None:intergenic
ACCTTGACATGAACAAACAA+AGG 0.644270 1_2:-68133272 None:intergenic
TTGGTATTCAGAGAAATGGA+CGG 0.649153 1_2:+68132797 None:intergenic
CAAGATACAGAAGCACAGCA+AGG 0.651758 1_2:-68134728 None:intergenic
GAGTGCCTCACAACGAGAGG+TGG 0.653972 1_2:-68134307 None:intergenic
AATCCTTTGTTAGAGCGCGA+TGG 0.654133 1_2:+68132836 Msa0087230:CDS
ACTTCCTCTTATGTAAGCGG+TGG 0.654302 1_2:-68134265 None:intergenic
GATGCCAAGCACACAACCTG+TGG 0.654695 1_2:-68134062 None:intergenic
TTAGCAAACTTGGACCACCT+GGG 0.656483 1_2:-68134783 None:intergenic
TGCTGCTAATCAGTTTACGA+AGG 0.658067 1_2:+68134497 Msa0087230:CDS
GGGACTCGCAAATTGCTGCA+AGG 0.660465 1_2:+68134847 Msa0087230:CDS
GAGGCACTCCATAGCCACAT+GGG 0.668287 1_2:+68134321 Msa0087230:CDS
TCTGTTATAACTGACAAAGG+AGG 0.671475 1_2:-68133988 None:intergenic
ATCCATTGGCATAACTACCA+CGG 0.671550 1_2:-68134815 None:intergenic
GTACGAAAAGAAGAAGATGT+GGG 0.675433 1_2:+68134242 Msa0087230:CDS
ACACCAATGTCAACTGACGA+AGG 0.680831 1_2:+68135215 Msa0087230:CDS
GCCAAACCCTACCATCACCA+CGG 0.683425 1_2:-68134623 None:intergenic
GAGGATGCGTACAACACCGA+GGG 0.685934 1_2:+68135251 Msa0087230:CDS
ACGATGATAGGAGGCTTGAG+CGG 0.692756 1_2:+68133737 Msa0087230:CDS
GGCTGTGGAGCTAACCCCAG+TGG 0.693658 1_2:+68135083 Msa0087230:CDS
TTGCGACTGGTATAGTAACG+CGG 0.700252 1_2:+68133905 Msa0087230:CDS
AAGACCGTACCAAATGTATG+GGG 0.707217 1_2:+68133543 Msa0087230:CDS
TTCATGTCAAGGTTACAGGG+AGG 0.709283 1_2:+68133282 Msa0087230:CDS
AATGCACGTATTGTCCCATG+TGG 0.722187 1_2:-68134335 None:intergenic
ACAGCAAGGATATGATCACA+CGG 0.726193 1_2:-68134714 None:intergenic
ATGTTAGGAGGTACTCTGTG+TGG 0.729376 1_2:+68133448 Msa0087230:CDS
CGAGGATGCGTACAACACCG+AGG 0.735213 1_2:+68135250 Msa0087230:CDS
AAAGGCAAGAAATGTCCATG+GGG 0.742239 1_2:+68134577 Msa0087230:CDS
GTCTACCAGGAGTACCACTG+GGG 0.788532 1_2:-68135097 None:intergenic
CGTGTTGTCGAATATCTGAG+CGG 0.788889 1_2:+68134939 Msa0087230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAAAATACTTGTGATTTT+AGG + chr1_2:68135323-68135342 Msa0087230:intron 15.0%
!!! ATGTGAAATTTGAAATTGAA+TGG + chr1_2:68132889-68132908 Msa0087230:intron 20.0%
!!! TTTTAAATTAACTTAGCATG+TGG + chr1_2:68132914-68132933 Msa0087230:intron 20.0%
!!! CTAAAAGAAAATTTTCTTAC+AGG + chr1_2:68132937-68132956 Msa0087230:intron 20.0%
!!! ACCATATCTTTTATCATAAT+CGG - chr1_2:68133079-68133098 None:intergenic 20.0%
!! AATGAAAAATGCCATTAAAA+AGG + chr1_2:68134023-68134042 Msa0087230:CDS 20.0%
!! TTAACAAATTTGAAGATTTG+TGG + chr1_2:68134166-68134185 Msa0087230:CDS 20.0%
!!! TTAATCGGCTTAATTTAAAA+AGG + chr1_2:68134967-68134986 Msa0087230:CDS 20.0%
!!! CAAGTATTTTTAATGTTGTA+GGG - chr1_2:68135316-68135335 None:intergenic 20.0%
!!! ACAAGTATTTTTAATGTTGT+AGG - chr1_2:68135317-68135336 None:intergenic 20.0%
!!! TACTCAAATTTTTACGTATA+TGG + chr1_2:68135392-68135411 Msa0087230:CDS 20.0%
! TCCGATTATGATAAAAGATA+TGG + chr1_2:68133075-68133094 Msa0087230:CDS 25.0%
! CAAAAAATGAAACAAGATGT+GGG + chr1_2:68133341-68133360 Msa0087230:CDS 25.0%
! TAAAAAAGATTTGTACAACC+AGG + chr1_2:68133609-68133628 Msa0087230:CDS 25.0%
! CGTATGAACTATGAAATATT+TGG + chr1_2:68133784-68133803 Msa0087230:CDS 25.0%
! TTCAGATAAGACATAAAATC+AGG - chr1_2:68134120-68134139 None:intergenic 25.0%
! TTATCTGAAAAAATGCATGT+TGG + chr1_2:68134131-68134150 Msa0087230:CDS 25.0%
!!! AGAGGTGTTTAACATTTTTT+CGG + chr1_2:68134394-68134413 Msa0087230:CDS 25.0%
!!! TTTAACATTTTTTCGGTTCA+GGG + chr1_2:68134401-68134420 Msa0087230:CDS 25.0%
! TTTGATTACCAATCCAATTA+CGG + chr1_2:68134432-68134451 Msa0087230:CDS 25.0%
TGATATAATGAAGATTGACC+TGG + chr1_2:68133099-68133118 Msa0087230:CDS 30.0%
AAACCAACAGTAAAACATGT+AGG - chr1_2:68133179-68133198 None:intergenic 30.0%
!!! CCACATCTTGTTTCATTTTT+TGG - chr1_2:68133343-68133362 None:intergenic 30.0%
CCAAAAAATGAAACAAGATG+TGG + chr1_2:68133340-68133359 Msa0087230:CDS 30.0%
GATCACAATCATGTTATGTT+AGG + chr1_2:68133433-68133452 Msa0087230:CDS 30.0%
!!! CAATTTTTGAGTGCAATGAA+CGG + chr1_2:68133961-68133980 Msa0087230:CDS 30.0%
! TAATGGCATTTTTCATTGCA+AGG - chr1_2:68134020-68134039 None:intergenic 30.0%
! TGCAGGAAAAACCTTTTTAA+TGG - chr1_2:68134037-68134056 None:intergenic 30.0%
GAAAAAATGTTAAACACCTC+TGG - chr1_2:68134395-68134414 None:intergenic 30.0%
!!! GTTTAACATTTTTTCGGTTC+AGG + chr1_2:68134400-68134419 Msa0087230:CDS 30.0%
TGATGATCTTCCAAAATGTT+TGG + chr1_2:68134755-68134774 Msa0087230:CDS 30.0%
GATTGAATCTTTAGCAAACT+TGG - chr1_2:68134796-68134815 None:intergenic 30.0%
ACAAATTGCGAACAAAGCAA+TGG + chr1_2:68132992-68133011 Msa0087230:CDS 35.0%
GGAAGTTCAAATTCAGTTGA+AGG + chr1_2:68133025-68133044 Msa0087230:CDS 35.0%
TTCAAATTCAGTTGAAGGCT+TGG + chr1_2:68133030-68133049 Msa0087230:CDS 35.0%
! TTGCCTACATGTTTTACTGT+TGG + chr1_2:68133173-68133192 Msa0087230:CDS 35.0%
! TCAGGATTTTCAGTTCGTAA+GGG + chr1_2:68133208-68133227 Msa0087230:CDS 35.0%
! ACCTTTGTTTGTTCATGTCA+AGG + chr1_2:68133271-68133290 Msa0087230:CDS 35.0%
ACCTTGACATGAACAAACAA+AGG - chr1_2:68133275-68133294 None:intergenic 35.0%
TTTGTTCATGTCAAGGTTAC+AGG + chr1_2:68133278-68133297 Msa0087230:CDS 35.0%
TTGTTCATGTCAAGGTTACA+GGG + chr1_2:68133279-68133298 Msa0087230:CDS 35.0%
CACAATCATGTTATGTTAGG+AGG + chr1_2:68133436-68133455 Msa0087230:CDS 35.0%
!! ACACTCATTTTTCTATACGC+TGG - chr1_2:68133481-68133500 None:intergenic 35.0%
ATAAGACCGTACCAAATGTA+TGG + chr1_2:68133541-68133560 Msa0087230:CDS 35.0%
TAAGACCGTACCAAATGTAT+GGG + chr1_2:68133542-68133561 Msa0087230:CDS 35.0%
AAACAATGATCTACGTTGCT+TGG - chr1_2:68133638-68133657 None:intergenic 35.0%
! TCATTGTTTTCTGATGCTTG+TGG + chr1_2:68133649-68133668 Msa0087230:CDS 35.0%
! CAGCTTTTCACAAACATTAG+AGG - chr1_2:68133712-68133731 None:intergenic 35.0%
! AAAGCTGTATTGACGATGAT+AGG + chr1_2:68133725-68133744 Msa0087230:CDS 35.0%
TATACCAGTCGCAAAAACAA+TGG - chr1_2:68133899-68133918 None:intergenic 35.0%
AGACCGAAGAAACATATGTA+TGG + chr1_2:68133929-68133948 Msa0087230:CDS 35.0%
CAGCCATACATATGTTTCTT+CGG - chr1_2:68133935-68133954 None:intergenic 35.0%
AGAAACATATGTATGGCTGT+TGG + chr1_2:68133936-68133955 Msa0087230:CDS 35.0%
TCTGTTATAACTGACAAAGG+AGG - chr1_2:68133991-68134010 None:intergenic 35.0%
CCATCTGTTATAACTGACAA+AGG - chr1_2:68133994-68134013 None:intergenic 35.0%
CCTTTGTCAGTTATAACAGA+TGG + chr1_2:68133991-68134010 Msa0087230:CDS 35.0%
AAAATCAGGAATCCCAATGT+TGG - chr1_2:68134106-68134125 None:intergenic 35.0%
TCAGAGATGGTTCAAAAGTT+TGG + chr1_2:68134189-68134208 Msa0087230:CDS 35.0%
CAGAGATGGTTCAAAAGTTT+GGG + chr1_2:68134190-68134209 Msa0087230:CDS 35.0%
TGTACGAAAAGAAGAAGATG+TGG + chr1_2:68134241-68134260 Msa0087230:CDS 35.0%
GTACGAAAAGAAGAAGATGT+GGG + chr1_2:68134242-68134261 Msa0087230:CDS 35.0%
GAAACTTCCTCTTATGTAAG+CGG - chr1_2:68134271-68134290 None:intergenic 35.0%
ATAAGAGGAAGTTTCTTTGC+TGG + chr1_2:68134276-68134295 Msa0087230:CDS 35.0%
TTTCTCTAATGACCGTAAAC+TGG - chr1_2:68134475-68134494 None:intergenic 35.0%
TGCTTCTTAGAGTTACAGAA+AGG + chr1_2:68134559-68134578 Msa0087230:CDS 35.0%
CACGAGCATTTCAAAATAAC+TGG - chr1_2:68134669-68134688 None:intergenic 35.0%
TTTGCTAAAGATTCAATCCG+TGG + chr1_2:68134798-68134817 Msa0087230:CDS 35.0%
CCAAATGTGTAAAGTTGTTG+CGG + chr1_2:68134884-68134903 Msa0087230:CDS 35.0%
ATCTGAGCGGTGAAATTAAT+CGG + chr1_2:68134952-68134971 Msa0087230:CDS 35.0%
TCCACAATGTGTAAGAACAA+AGG + chr1_2:68135061-68135080 Msa0087230:CDS 35.0%
CCACAATGTGTAAGAACAAA+GGG + chr1_2:68135062-68135081 Msa0087230:CDS 35.0%
TTCACTTACATTGTAACCCT+CGG - chr1_2:68135270-68135289 None:intergenic 35.0%
!!! CAGGATTTTGAAGCTGAAAG+TGG + chr1_2:68132956-68132975 Msa0087230:intron 40.0%
!!! GATTTTGAAGCTGAAAGTGG+AGG + chr1_2:68132959-68132978 Msa0087230:CDS 40.0%
CAAAGCAATGGAGATGTCAA+TGG + chr1_2:68133004-68133023 Msa0087230:CDS 40.0%
AAATTCAGTTGAAGGCTTGG+AGG + chr1_2:68133033-68133052 Msa0087230:CDS 40.0%
! TGACCTGGAAAACTTTTGTG+TGG + chr1_2:68133114-68133133 Msa0087230:CDS 40.0%
! AAACTTTTGTGTGGCTGATG+TGG + chr1_2:68133123-68133142 Msa0087230:CDS 40.0%
AACTGAAAATCCTGACGTTC+TGG - chr1_2:68133203-68133222 None:intergenic 40.0%
! GTCAGGATTTTCAGTTCGTA+AGG + chr1_2:68133207-68133226 Msa0087230:CDS 40.0%
! CAGGATTTTCAGTTCGTAAG+GGG + chr1_2:68133209-68133228 Msa0087230:CDS 40.0%
ATGAACAAACAAAGGTCTGC+TGG - chr1_2:68133267-68133286 None:intergenic 40.0%
!!! CATTTTTTGGCTCACGCTTT+CGG - chr1_2:68133330-68133349 None:intergenic 40.0%
! TGCAAAATTTCGAGTCCACT+TGG + chr1_2:68133369-68133388 Msa0087230:CDS 40.0%
! TCCACTTGGATATTTTGTCG+AGG + chr1_2:68133383-68133402 Msa0087230:CDS 40.0%
! TGGATATTTTGTCGAGGCAT+TGG + chr1_2:68133389-68133408 Msa0087230:CDS 40.0%
ACGCTCAATTTCAGCTATGT+CGG - chr1_2:68133506-68133525 None:intergenic 40.0%
AAGACCGTACCAAATGTATG+GGG + chr1_2:68133543-68133562 Msa0087230:CDS 40.0%
AAAGCCCCATACATTTGGTA+CGG - chr1_2:68133550-68133569 None:intergenic 40.0%
GACAGTTGTACTTGTTCTTC+CGG - chr1_2:68133837-68133856 None:intergenic 40.0%
ACACCTGAGAACACAACAAA+CGG - chr1_2:68133859-68133878 None:intergenic 40.0%
TGAAGATTTGTGGTCAGAGA+TGG + chr1_2:68134176-68134195 Msa0087230:CDS 40.0%
GATGGTTCAAAAGTTTGGGT+TGG + chr1_2:68134194-68134213 Msa0087230:CDS 40.0%
!! TTGGGTTGGAAAACAACAGT+TGG + chr1_2:68134208-68134227 Msa0087230:CDS 40.0%
! ATTTTGTGCAACAGTTCCAG+AGG + chr1_2:68134376-68134395 Msa0087230:CDS 40.0%
TGCTGCTAATCAGTTTACGA+AGG + chr1_2:68134497-68134516 Msa0087230:CDS 40.0%
TGCTAATCAGTTTACGAAGG+CGG + chr1_2:68134500-68134519 Msa0087230:CDS 40.0%
! AAGCAGTGATCTTTTCAGCA+CGG - chr1_2:68134544-68134563 None:intergenic 40.0%
AGAAAGGCAAGAAATGTCCA+TGG + chr1_2:68134575-68134594 Msa0087230:CDS 40.0%
GAAAGGCAAGAAATGTCCAT+GGG + chr1_2:68134576-68134595 Msa0087230:CDS 40.0%
AAAGGCAAGAAATGTCCATG+GGG + chr1_2:68134577-68134596 Msa0087230:CDS 40.0%
!! GTCCATGGGGTTTATTTTCA+CGG + chr1_2:68134590-68134609 Msa0087230:CDS 40.0%
AACCGTGAAAATAAACCCCA+TGG - chr1_2:68134595-68134614 None:intergenic 40.0%
TTCACGGTTTCAAAATACCG+TGG + chr1_2:68134606-68134625 Msa0087230:CDS 40.0%
GTTTCAAAATACCGTGGTGA+TGG + chr1_2:68134612-68134631 Msa0087230:CDS 40.0%
!! AAAATACCGTGGTGATGGTA+GGG + chr1_2:68134617-68134636 Msa0087230:CDS 40.0%
GAAATGCTCGTGTCTTAGAA+TGG + chr1_2:68134677-68134696 Msa0087230:CDS 40.0%
TGTCTTAGAATGGAATCGCT+TGG + chr1_2:68134687-68134706 Msa0087230:CDS 40.0%
ACAGCAAGGATATGATCACA+CGG - chr1_2:68134717-68134736 None:intergenic 40.0%
! CTGGGCAAAACCAAACATTT+TGG - chr1_2:68134768-68134787 None:intergenic 40.0%
!!! CAAAATGTTTGGTTTTGCCC+AGG + chr1_2:68134766-68134785 Msa0087230:CDS 40.0%
ATCCATTGGCATAACTACCA+CGG - chr1_2:68134818-68134837 None:intergenic 40.0%
ATGCCAATGGATCTCTCTAT+TGG + chr1_2:68134826-68134845 Msa0087230:CDS 40.0%
TGCCAATGGATCTCTCTATT+GGG + chr1_2:68134827-68134846 Msa0087230:CDS 40.0%
! CAAATTGCTGCAAGGTATTC+TGG + chr1_2:68134855-68134874 Msa0087230:CDS 40.0%
CCGCAACAACTTTACACATT+TGG - chr1_2:68134887-68134906 None:intergenic 40.0%
AATTTGTGAACCAGTTCCAG+TGG + chr1_2:68134998-68135017 Msa0087230:CDS 40.0%
CCCTTTGTTCTTACACATTG+TGG - chr1_2:68135065-68135084 None:intergenic 40.0%
ATTCCTTCGTCAGTTGACAT+TGG - chr1_2:68135221-68135240 None:intergenic 40.0%
!! CTCGTTCTTACTTGTTGTCT+AGG + chr1_2:68135365-68135384 Msa0087230:intron 40.0%
AATCCTTTGTTAGAGCGCGA+TGG + chr1_2:68132836-68132855 Msa0087230:CDS 45.0%
! CAGCCACACAAAAGTTTTCC+AGG - chr1_2:68133120-68133139 None:intergenic 45.0%
TTCATGTCAAGGTTACAGGG+AGG + chr1_2:68133282-68133301 Msa0087230:CDS 45.0%
TCAAGGTTACAGGGAGGATA+GGG + chr1_2:68133288-68133307 Msa0087230:CDS 45.0%
GCCTCGACAAAATATCCAAG+TGG - chr1_2:68133387-68133406 None:intergenic 45.0%
!! ATGTTAGGAGGTACTCTGTG+TGG + chr1_2:68133448-68133467 Msa0087230:CDS 45.0%
ATTGAGCGTAGTCGAAAAGC+TGG + chr1_2:68133517-68133536 Msa0087230:CDS 45.0%
GTACCAAATGTATGGGGCTT+TGG + chr1_2:68133549-68133568 Msa0087230:CDS 45.0%
!! TGCTTCTGGGGGTTTTCATA+AGG + chr1_2:68133576-68133595 Msa0087230:CDS 45.0%
! TCTGGGGGTTTTCATAAGGT+AGG + chr1_2:68133580-68133599 Msa0087230:CDS 45.0%
! GCTGTATTGACGATGATAGG+AGG + chr1_2:68133728-68133747 Msa0087230:CDS 45.0%
GTTGTACTTGTTCTTCCGGT+AGG - chr1_2:68133833-68133852 None:intergenic 45.0%
!! TGTCCGTTTGTTGTGTTCTC+AGG + chr1_2:68133853-68133872 Msa0087230:CDS 45.0%
!!! CAGACCATTGTTTTTGCGAC+TGG + chr1_2:68133892-68133911 Msa0087230:CDS 45.0%
TTGCGACTGGTATAGTAACG+CGG + chr1_2:68133905-68133924 Msa0087230:CDS 45.0%
TGTGTGCTTGGCATCTGTTA+AGG + chr1_2:68134070-68134089 Msa0087230:CDS 45.0%
AGGAATGCTTCCACCAACAT+TGG + chr1_2:68134090-68134109 Msa0087230:CDS 45.0%
GGAATGCTTCCACCAACATT+GGG + chr1_2:68134091-68134110 Msa0087230:CDS 45.0%
ATCAGGAATCCCAATGTTGG+TGG - chr1_2:68134103-68134122 None:intergenic 45.0%
ACTTCCTCTTATGTAAGCGG+TGG - chr1_2:68134268-68134287 None:intergenic 45.0%
AATGCACGTATTGTCCCATG+TGG - chr1_2:68134338-68134357 None:intergenic 45.0%
TGGGACAATACGTGCATTCT+CGG + chr1_2:68134340-68134359 Msa0087230:CDS 45.0%
CAGGAAGACCGTAATTGGAT+TGG - chr1_2:68134443-68134462 None:intergenic 45.0%
CATCACAGGAAGACCGTAAT+TGG - chr1_2:68134448-68134467 None:intergenic 45.0%
CTGTGATGCAGACCAGTTTA+CGG + chr1_2:68134460-68134479 Msa0087230:CDS 45.0%
!! CAAAATACCGTGGTGATGGT+AGG + chr1_2:68134616-68134635 Msa0087230:CDS 45.0%
! CAAGATACAGAAGCACAGCA+AGG - chr1_2:68134731-68134750 None:intergenic 45.0%
!!! AATGTTTGGTTTTGCCCAGG+TGG + chr1_2:68134769-68134788 Msa0087230:CDS 45.0%
TTAGCAAACTTGGACCACCT+GGG - chr1_2:68134786-68134805 None:intergenic 45.0%
TTTAGCAAACTTGGACCACC+TGG - chr1_2:68134787-68134806 None:intergenic 45.0%
ATCCGTGGTAGTTATGCCAA+TGG + chr1_2:68134813-68134832 Msa0087230:CDS 45.0%
GTCCCAATAGAGAGATCCAT+TGG - chr1_2:68134832-68134851 None:intergenic 45.0%
GTGTAAAGTTGTTGCGGAGA+TGG + chr1_2:68134890-68134909 Msa0087230:CDS 45.0%
CGCTCAGATATTCGACAACA+CGG - chr1_2:68134941-68134960 None:intergenic 45.0%
CGTGTTGTCGAATATCTGAG+CGG + chr1_2:68134939-68134958 Msa0087230:CDS 45.0%
CGAGAATGCTGAGAATGTGT+TGG + chr1_2:68135037-68135056 Msa0087230:CDS 45.0%
TGTGTAAGAACAAAGGGCTG+TGG + chr1_2:68135068-68135087 Msa0087230:CDS 45.0%
ACACAAACTTGCAGCGTTTG+TGG + chr1_2:68135128-68135147 Msa0087230:CDS 45.0%
ACATTGATGCGACTGATGGA+AGG - chr1_2:68135182-68135201 None:intergenic 45.0%
TCCATCAGTCGCATCAATGT+TGG + chr1_2:68135182-68135201 Msa0087230:CDS 45.0%
CCATCAGTCGCATCAATGTT+GGG + chr1_2:68135183-68135202 Msa0087230:CDS 45.0%
ACACCAATGTCAACTGACGA+AGG + chr1_2:68135215-68135234 Msa0087230:CDS 45.0%
ACACCATCGCGCTCTAACAA+AGG - chr1_2:68132842-68132861 None:intergenic 50.0%
!! TGTTGGTTTGCCAGAACGTC+AGG + chr1_2:68133190-68133209 Msa0087230:CDS 50.0%
TTCAGTTCGTAAGGGGCAAG+TGG + chr1_2:68133216-68133235 Msa0087230:CDS 50.0%
CAAGTGGTGAGGGATCGAAA+TGG + chr1_2:68133232-68133251 Msa0087230:CDS 50.0%
GTCAAGGTTACAGGGAGGAT+AGG + chr1_2:68133287-68133306 Msa0087230:CDS 50.0%
CAAGGTTACAGGGAGGATAG+GGG + chr1_2:68133289-68133308 Msa0087230:CDS 50.0%
AAGGTTACAGGGAGGATAGG+GGG + chr1_2:68133290-68133309 Msa0087230:CDS 50.0%
GGAGGATAGGGGGTTAACAA+TGG + chr1_2:68133300-68133319 Msa0087230:CDS 50.0%
CTGCCAAAGCCCCATACATT+TGG - chr1_2:68133555-68133574 None:intergenic 50.0%
ATCTACGTTGCTTGGCAACC+TGG - chr1_2:68133630-68133649 None:intergenic 50.0%
ACGATGATAGGAGGCTTGAG+CGG + chr1_2:68133737-68133756 Msa0087230:CDS 50.0%
TAGCCTTCGATGCAACCTAC+CGG + chr1_2:68133815-68133834 Msa0087230:CDS 50.0%
TGCGACTGGTATAGTAACGC+GGG + chr1_2:68133906-68133925 Msa0087230:CDS 50.0%
AAGAACCACCTCTCGTTGTG+AGG + chr1_2:68134302-68134321 Msa0087230:CDS 50.0%
CGTATTGTCCCATGTGGCTA+TGG - chr1_2:68134332-68134351 None:intergenic 50.0%
! CGTAAACTGGTCTGCATCAC+AGG - chr1_2:68134462-68134481 None:intergenic 50.0%
!! CTGTTTTGTGCCACTGGAAC+TGG - chr1_2:68135011-68135030 None:intergenic 50.0%
!!! TCTGTGCTGTTTTGTGCCAC+TGG - chr1_2:68135017-68135036 None:intergenic 50.0%
! TTGCAGCGTTTGTGGTGTCA+TGG + chr1_2:68135136-68135155 Msa0087230:CDS 50.0%
!! TGCAGCGTTTGTGGTGTCAT+GGG + chr1_2:68135137-68135156 Msa0087230:CDS 50.0%
CCCAACATTGATGCGACTGA+TGG - chr1_2:68135186-68135205 None:intergenic 50.0%
CGAAGGAATTCTCCAAGACG+AGG + chr1_2:68135232-68135251 Msa0087230:CDS 50.0%
TGTTGTACGCATCCTCGTCT+TGG - chr1_2:68135247-68135266 None:intergenic 50.0%
TTCGTAAGGGGCAAGTGGTG+AGG + chr1_2:68133221-68133240 Msa0087230:CDS 55.0%
TCGTAAGGGGCAAGTGGTGA+GGG + chr1_2:68133222-68133241 Msa0087230:CDS 55.0%
! GGGCTTTGGCAGATGCTTCT+GGG + chr1_2:68133563-68133582 Msa0087230:CDS 55.0%
! GGCTTTGGCAGATGCTTCTG+GGG + chr1_2:68133564-68133583 Msa0087230:CDS 55.0%
GCTTTGGCAGATGCTTCTGG+GGG + chr1_2:68133565-68133584 Msa0087230:CDS 55.0%
GAGCGGTTGTTCTGGTGTGA+CGG + chr1_2:68133754-68133773 Msa0087230:CDS 55.0%
CTTCCGGTAGGTTGCATCGA+AGG - chr1_2:68133821-68133840 None:intergenic 55.0%
! TTTTTCCTGCAGCGCACCAC+AGG + chr1_2:68134046-68134065 Msa0087230:CDS 55.0%
GATGCCAAGCACACAACCTG+TGG - chr1_2:68134065-68134084 None:intergenic 55.0%
TGGGCCACCGCTTACATAAG+AGG + chr1_2:68134261-68134280 Msa0087230:CDS 55.0%
! ATGGAGTGCCTCACAACGAG+AGG - chr1_2:68134313-68134332 None:intergenic 55.0%
! TGAGGCACTCCATAGCCACA+TGG + chr1_2:68134320-68134339 Msa0087230:CDS 55.0%
! GAGGCACTCCATAGCCACAT+GGG + chr1_2:68134321-68134340 Msa0087230:CDS 55.0%
!! ACCGTGGTGATGGTAGGGTT+TGG + chr1_2:68134622-68134641 Msa0087230:CDS 55.0%
GCCAAACCCTACCATCACCA+CGG - chr1_2:68134626-68134645 None:intergenic 55.0%
GGGACTCGCAAATTGCTGCA+AGG + chr1_2:68134847-68134866 Msa0087230:CDS 55.0%
!! GTCTACCAGGAGTACCACTG+GGG - chr1_2:68135100-68135119 None:intergenic 55.0%
!! CGTCTACCAGGAGTACCACT+GGG - chr1_2:68135101-68135120 None:intergenic 55.0%
GAGGATGCGTACAACACCGA+GGG + chr1_2:68135251-68135270 Msa0087230:CDS 55.0%
! GGGGCTTTGGCAGATGCTTC+TGG + chr1_2:68133562-68133581 Msa0087230:CDS 60.0%
GGAGGCTTGAGCGGTTGTTC+TGG + chr1_2:68133746-68133765 Msa0087230:CDS 60.0%
CGCACCACAGGTTGTGTGCT+TGG + chr1_2:68134058-68134077 Msa0087230:CDS 60.0%
GAGTGCCTCACAACGAGAGG+TGG - chr1_2:68134310-68134329 None:intergenic 60.0%
GCTAACCCCAGTGGTACTCC+TGG + chr1_2:68135092-68135111 Msa0087230:CDS 60.0%
!! GCGTCTACCAGGAGTACCAC+TGG - chr1_2:68135102-68135121 None:intergenic 60.0%
TGTGTGCGTCTGCGTCTACC+AGG - chr1_2:68135113-68135132 None:intergenic 60.0%
CGAGGATGCGTACAACACCG+AGG + chr1_2:68135250-68135269 Msa0087230:CDS 60.0%
CACAACCTGTGGTGCGCTGC+AGG - chr1_2:68134054-68134073 None:intergenic 65.0%
GGCTGTGGAGCTAACCCCAG+TGG + chr1_2:68135083-68135102 Msa0087230:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 68132812 68135425 68132812 ID=Msa0087230;Name=Msa0087230
chr1_2 mRNA 68132812 68135425 68132812 ID=Msa0087230-mRNA-1;Parent=Msa0087230;Name=Msa0087230-mRNA-1;_AED=0.49;_eAED=0.49;_QI=0|0.5|0|0.66|1|1|3|0|807
chr1_2 exon 68132812 68132874 68132812 ID=Msa0087230-mRNA-1:exon:23201;Parent=Msa0087230-mRNA-1
chr1_2 exon 68132959 68135280 68132959 ID=Msa0087230-mRNA-1:exon:23202;Parent=Msa0087230-mRNA-1
chr1_2 exon 68135387 68135425 68135387 ID=Msa0087230-mRNA-1:exon:23203;Parent=Msa0087230-mRNA-1
chr1_2 CDS 68132812 68132874 68132812 ID=Msa0087230-mRNA-1:cds;Parent=Msa0087230-mRNA-1
chr1_2 CDS 68132959 68135280 68132959 ID=Msa0087230-mRNA-1:cds;Parent=Msa0087230-mRNA-1
chr1_2 CDS 68135387 68135425 68135387 ID=Msa0087230-mRNA-1:cds;Parent=Msa0087230-mRNA-1
Gene Sequence

>Msa0087230

ATGGACGGACGTTCAGAGGCTGTGAATCCTTTGTTAGAGCGCGATGGTGTGAGAATTTCAAGAGATTTTGAAGCTGAAAGTGGAGGTAATTACAACACAAATTGCGAACAAAGCAATGGAGATGTCAATGGAAGTTCAAATTCAGTTGAAGGCTTGGAGGATGAAACTAATGAAGTCAATCCGATTATGATAAAAGATATGGTTGATATAATGAAGATTGACCTGGAAAACTTTTGTGTGGCTGATGTGGAAAGATATGAATTTGTTGCTTTAGATGTTGCCTACATGTTTTACTGTTGGTTTGCCAGAACGTCAGGATTTTCAGTTCGTAAGGGGCAAGTGGTGAGGGATCGAAATGGTGAAGTCGTCCAGCAGACCTTTGTTTGTTCATGTCAAGGTTACAGGGAGGATAGGGGGTTAACAATGGAAAACCGAAAGCGTGAGCCAAAAAATGAAACAAGATGTGGGTGTAATGCAAAATTTCGAGTCCACTTGGATATTTTGTCGAGGCATTGGTATGTTACTGTATTTGAATTTGATCACAATCATGTTATGTTAGGAGGTACTCTGTGTGGAATGTTGCCAGCGTATAGAAAAATGAGTGTAGCCGACATAGCTGAAATTGAGCGTAGTCGAAAAGCTGGTATAAGACCGTACCAAATGTATGGGGCTTTGGCAGATGCTTCTGGGGGTTTTCATAAGGTAGGATTTGTTAAAAAAGATTTGTACAACCAGGTTGCCAAGCAACGTAGATCATTGTTTTCTGATGCTTGTGGCGCAGTTAAGTATCTTCGAGATCTTTGCAAAGACGATCCTCTAATGTTTGTGAAAAGCTGTATTGACGATGATAGGAGGCTTGAGCGGTTGTTCTGGTGTGACGGTGAAAGTCGTATGAACTATGAAATATTTGGAGATGTTTTAGCCTTCGATGCAACCTACCGGAAGAACAAGTACAACTGTCCGTTTGTTGTGTTCTCAGGTGTTAATCATCATAATCAGACCATTGTTTTTGCGACTGGTATAGTAACGCGGGAGACCGAAGAAACATATGTATGGCTGTTGGAACAATTTTTGAGTGCAATGAACGGCAAACCTCCTTTGTCAGTTATAACAGATGGCGACCTTGCAATGAAAAATGCCATTAAAAAGGTTTTTCCTGCAGCGCACCACAGGTTGTGTGCTTGGCATCTGTTAAGGAATGCTTCCACCAACATTGGGATTCCTGATTTTATGTCTTATCTGAAAAAATGCATGTTGGCTGACATTGAGATTAACAAATTTGAAGATTTGTGGTCAGAGATGGTTCAAAAGTTTGGGTTGGAAAACAACAGTTGGATCAATGAGATGTACGAAAAGAAGAAGATGTGGGCCACCGCTTACATAAGAGGAAGTTTCTTTGCTGGTATAAGAACCACCTCTCGTTGTGAGGCACTCCATAGCCACATGGGACAATACGTGCATTCTCGGATAAACATGACTGATTTTGTGCAACAGTTCCAGAGGTGTTTAACATTTTTTCGGTTCAGGGAAATTTTATTTGATTACCAATCCAATTACGGTCTTCCTGTGATGCAGACCAGTTTACGGTCATTAGAGAAATCTGCTGCTAATCAGTTTACGAAGGCGGTCTTTCTACTGTTTCGTACCGTGCTGAAAAGATCACTGCTTCTTAGAGTTACAGAAAGGCAAGAAATGTCCATGGGGTTTATTTTCACGGTTTCAAAATACCGTGGTGATGGTAGGGTTTGGCATGTGACATTCTGTGAAGAACCAGTTATTTTGAAATGCTCGTGTCTTAGAATGGAATCGCTTGGTTTGCCGTGTGATCATATCCTTGCTGTGCTTCTGTATCTTGATTTTGATGATCTTCCAAAATGTTTGGTTTTGCCCAGGTGGTCCAAGTTTGCTAAAGATTCAATCCGTGGTAGTTATGCCAATGGATCTCTCTATTGGGACTCGCAAATTGCTGCAAGGTATTCTGGCATAGTCCAAATGTGTAAAGTTGTTGCGGAGATGGTTTACGAAGATGAAGATGAGTACAACCGTGTTGTCGAATATCTGAGCGGTGAAATTAATCGGCTTAATTTAAAAAGGAACAACGCAATTTGTGAACCAGTTCCAGTGGCACAAAACAGCACAGACGAGAATGCTGAGAATGTGTTGGATCCACAATGTGTAAGAACAAAGGGCTGTGGAGCTAACCCCAGTGGTACTCCTGGTAGACGCAGACGCACACAAACTTGCAGCGTTTGTGGTGTCATGGGTCACAATAGACGTTGTTGTCCTTCCATCAGTCGCATCAATGTTGGGCAGACACAAACACCAATGTCAACTGACGAAGGAATTCTCCAAGACGAGGATGCGTACAACACCGAGGGTTACAATGTGTATACTCAAATTTTTACGTATATGGTTAATCATTAA
Protein sequence

>Msa0087230

MDGRSEAVNPLLERDGVRISRDFEAESGGNYNTNCEQSNGDVNGSSNSVEGLEDETNEVNPIMIKDMVDIMKIDLENFCVADVERYEFVALDVAYMFYCWFARTSGFSVRKGQVVRDRNGEVVQQTFVCSCQGYREDRGLTMENRKREPKNETRCGCNAKFRVHLDILSRHWYVTVFEFDHNHVMLGGTLCGMLPAYRKMSVADIAEIERSRKAGIRPYQMYGALADASGGFHKVGFVKKDLYNQVAKQRRSLFSDACGAVKYLRDLCKDDPLMFVKSCIDDDRRLERLFWCDGESRMNYEIFGDVLAFDATYRKNKYNCPFVVFSGVNHHNQTIVFATGIVTRETEETYVWLLEQFLSAMNGKPPLSVITDGDLAMKNAIKKVFPAAHHRLCAWHLLRNASTNIGIPDFMSYLKKCMLADIEINKFEDLWSEMVQKFGLENNSWINEMYEKKKMWATAYIRGSFFAGIRTTSRCEALHSHMGQYVHSRINMTDFVQQFQRCLTFFRFREILFDYQSNYGLPVMQTSLRSLEKSAANQFTKAVFLLFRTVLKRSLLLRVTERQEMSMGFIFTVSKYRGDGRVWHVTFCEEPVILKCSCLRMESLGLPCDHILAVLLYLDFDDLPKCLVLPRWSKFAKDSIRGSYANGSLYWDSQIAARYSGIVQMCKVVAEMVYEDEDEYNRVVEYLSGEINRLNLKRNNAICEPVPVAQNSTDENAENVLDPQCVRTKGCGANPSGTPGRRRRTQTCSVCGVMGHNRRCCPSISRINVGQTQTPMSTDEGILQDEDAYNTEGYNVYTQIFTYMVNH*