Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0087930 | sp|P05332|YP20_BACLI | 34.503 | 171 | 103 | 3 | 24 | 188 | 8 | 175 | 4.39e-25 | 99.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0087930 | A0A072VP71 | 96.316 | 190 | 7 | 0 | 2 | 191 | 1 | 190 | 1.85e-131 | 375 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0087930 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0087930 | MtrunA17_Chr1g0204971 | 96.316 | 190 | 7 | 0 | 2 | 191 | 1 | 190 | 3.56e-135 | 375 |
| Msa0087930 | MtrunA17_Chr1g0204951 | 83.158 | 190 | 32 | 0 | 2 | 191 | 1 | 190 | 3.29e-116 | 328 |
| Msa0087930 | MtrunA17_Chr5g0394621 | 61.458 | 192 | 70 | 2 | 2 | 191 | 1 | 190 | 9.72e-82 | 240 |
| Msa0087930 | MtrunA17_Chr5g0394631 | 60.656 | 183 | 68 | 2 | 2 | 182 | 1 | 181 | 5.33e-73 | 225 |
| Msa0087930 | MtrunA17_Chr5g0394631 | 53.299 | 197 | 84 | 5 | 1 | 191 | 207 | 401 | 5.10e-64 | 202 |
| Msa0087930 | MtrunA17_Chr7g0273661 | 48.077 | 156 | 76 | 2 | 20 | 173 | 4 | 156 | 1.10e-44 | 145 |
| Msa0087930 | MtrunA17_Chr7g0223361 | 39.181 | 171 | 102 | 2 | 18 | 188 | 2 | 170 | 2.64e-38 | 129 |
| Msa0087930 | MtrunA17_Chr7g0223351 | 40.964 | 166 | 95 | 3 | 23 | 188 | 7 | 169 | 7.57e-37 | 126 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0087930 | AT2G32030.1 | 45.503 | 189 | 98 | 3 | 1 | 188 | 1 | 185 | 4.42e-55 | 173 |
| Msa0087930 | AT2G32020.1 | 51.497 | 167 | 76 | 3 | 23 | 188 | 18 | 180 | 1.08e-54 | 172 |
| Msa0087930 | AT3G22560.1 | 39.655 | 174 | 98 | 2 | 19 | 188 | 2 | 172 | 8.37e-36 | 124 |
Find 51 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGGTTGATGCTGCATTTA+AGG | 0.177178 | 1_2:-68954160 | Msa0087930:CDS |
| TTCTTTGAGCTCCATGTATT+GGG | 0.213171 | 1_2:-68954214 | Msa0087930:CDS |
| CTCTTCCTTGGCATCTGATT+TGG | 0.234671 | 1_2:+68954509 | None:intergenic |
| ATTCTTTGAGCTCCATGTAT+TGG | 0.293943 | 1_2:-68954215 | Msa0087930:CDS |
| TGCCTTCGCGATCGAGCAAT+TGG | 0.297095 | 1_2:-68954297 | Msa0087930:CDS |
| GCCTCAAGCCTCTCCAAGTA+TGG | 0.329009 | 1_2:+68954132 | None:intergenic |
| GAGATTTGCTTGCTATGTTT+TGG | 0.329625 | 1_2:+68954337 | None:intergenic |
| CCTTATACAAACAAAGAAAA+AGG | 0.343403 | 1_2:-68954372 | Msa0087930:CDS |
| GGGCAAAGGGATTGCCACTA+TGG | 0.352044 | 1_2:-68954193 | Msa0087930:CDS |
| TCAAGATCAGAAAGACTAAT+TGG | 0.352059 | 1_2:+68954450 | None:intergenic |
| CTTGGCATCTGATTTGGAAT+AGG | 0.366431 | 1_2:+68954515 | None:intergenic |
| AATTGGGTGTACCGAGTTCC+AGG | 0.375481 | 1_2:-68954280 | Msa0087930:CDS |
| CAGAAAATATTTGTTCATCA+AGG | 0.394310 | 1_2:-68954037 | Msa0087930:CDS |
| ATTTAAGGAGTTTCCATACT+TGG | 0.408342 | 1_2:-68954145 | Msa0087930:CDS |
| ACATAGCAAGCAAATCTCTA+TGG | 0.412043 | 1_2:-68954332 | Msa0087930:CDS |
| TACCGAGTTCCAGGTGTATT+CGG | 0.413001 | 1_2:-68954271 | Msa0087930:CDS |
| TAATTGGTCGAAGAGAGATT+TGG | 0.457252 | 1_2:+68954466 | None:intergenic |
| CGGGACAAATCTGCTCAACT+TGG | 0.459831 | 1_2:-68954240 | Msa0087930:CDS |
| GCCTTCGCGATCGAGCAATT+GGG | 0.464374 | 1_2:-68954296 | Msa0087930:CDS |
| CAAAGGGTGTTGGAGAAGGC+TGG | 0.494841 | 1_2:-68954081 | Msa0087930:CDS |
| AGCTCCATGTATTGGGGCAA+AGG | 0.494926 | 1_2:-68954207 | Msa0087930:CDS |
| AATCCCTTTGCCCCAATACA+TGG | 0.496567 | 1_2:+68954203 | None:intergenic |
| AGAAAATATTTGTTCATCAA+GGG | 0.502192 | 1_2:-68954036 | Msa0087930:CDS |
| CACTTGTTTCACAACCATAG+TGG | 0.504807 | 1_2:+68954179 | None:intergenic |
| TGGAGAAGGCTGGCTTTGAA+AGG | 0.513548 | 1_2:-68954071 | Msa0087930:CDS |
| AACAACAGAATACTCTTCCT+TGG | 0.516633 | 1_2:+68954497 | None:intergenic |
| GCTCCATGTATTGGGGCAAA+GGG | 0.517436 | 1_2:-68954206 | Msa0087930:CDS |
| GGAGAAGGCTGGCTTTGAAA+GGG | 0.520575 | 1_2:-68954070 | Msa0087930:CDS |
| ATCTTGATGATGTTATGTTG+TGG | 0.524636 | 1_2:-68954434 | Msa0087930:CDS |
| AGGTTCCCAAGTGCAATACT+TGG | 0.527166 | 1_2:+68954392 | None:intergenic |
| CAAGTTGAGCAGATTTGTCC+CGG | 0.527345 | 1_2:+68954241 | None:intergenic |
| AGGTGTATTCGGATAGATGC+CGG | 0.527922 | 1_2:-68954260 | Msa0087930:CDS |
| TGTGGCTTCACAAAGGGTGT+TGG | 0.533258 | 1_2:-68954091 | Msa0087930:CDS |
| TTCACAAAGGGTGTTGGAGA+AGG | 0.546969 | 1_2:-68954085 | Msa0087930:CDS |
| TCCATACTTGGAGAGGCTTG+AGG | 0.557426 | 1_2:-68954133 | Msa0087930:CDS |
| GCTTGAGGCTCGAGTTGATG+TGG | 0.569120 | 1_2:-68954118 | Msa0087930:CDS |
| GGCATCTATCCGAATACACC+TGG | 0.571236 | 1_2:+68954262 | None:intergenic |
| AGGAGTTTCCATACTTGGAG+AGG | 0.574247 | 1_2:-68954140 | Msa0087930:CDS |
| GGTGTATTCGGATAGATGCC+GGG | 0.580751 | 1_2:-68954259 | Msa0087930:CDS |
| CTATTCCAAATCAGATGCCA+AGG | 0.586783 | 1_2:-68954514 | Msa0087930:CDS |
| TCTTTGAGCTCCATGTATTG+GGG | 0.587210 | 1_2:-68954213 | Msa0087930:CDS |
| AAGTGGCCAAGTATTGCACT+TGG | 0.595225 | 1_2:-68954398 | Msa0087930:CDS |
| GGAAAATGTGGCTTCACAAA+GGG | 0.602523 | 1_2:-68954097 | Msa0087930:CDS |
| GTGGACTAGTGATGCAAAAG+TGG | 0.603948 | 1_2:-68954415 | Msa0087930:CDS |
| ATCCGAATACACCTGGAACT+CGG | 0.612102 | 1_2:+68954269 | None:intergenic |
| ACCCAATTGCTCGATCGCGA+AGG | 0.639639 | 1_2:+68954295 | None:intergenic |
| TGGAAAATGTGGCTTCACAA+AGG | 0.640244 | 1_2:-68954098 | Msa0087930:CDS |
| AAGGCTGGCTTTGAAAGGGA+AGG | 0.642488 | 1_2:-68954066 | Msa0087930:CDS |
| TCGAGTTGATGTGGAAAATG+TGG | 0.669414 | 1_2:-68954109 | Msa0087930:CDS |
| AGTGGCCAAGTATTGCACTT+GGG | 0.670287 | 1_2:-68954397 | Msa0087930:CDS |
| CACTATGGTTGTGAAACAAG+TGG | 0.680187 | 1_2:-68954178 | Msa0087930:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAATATTTGTTCATCAA+GGG | - | chr1_2:68954479-68954498 | Msa0087930:CDS | 20.0% |
| !!! | CCTTTTTCTTTGTTTGTATA+AGG | + | chr1_2:68954146-68954165 | None:intergenic | 25.0% |
| ! | CCTTATACAAACAAAGAAAA+AGG | - | chr1_2:68954143-68954162 | Msa0087930:CDS | 25.0% |
| ! | CAGAAAATATTTGTTCATCA+AGG | - | chr1_2:68954478-68954497 | Msa0087930:CDS | 25.0% |
| TCAAGATCAGAAAGACTAAT+TGG | + | chr1_2:68954068-68954087 | None:intergenic | 30.0% | |
| !! | ATCTTGATGATGTTATGTTG+TGG | - | chr1_2:68954081-68954100 | Msa0087930:CDS | 30.0% |
| ATTTAAGGAGTTTCCATACT+TGG | - | chr1_2:68954370-68954389 | Msa0087930:CDS | 30.0% | |
| AACAACAGAATACTCTTCCT+TGG | + | chr1_2:68954021-68954040 | None:intergenic | 35.0% | |
| TAATTGGTCGAAGAGAGATT+TGG | + | chr1_2:68954052-68954071 | None:intergenic | 35.0% | |
| !!! | GAGATTTGCTTGCTATGTTT+TGG | + | chr1_2:68954181-68954200 | None:intergenic | 35.0% |
| ACATAGCAAGCAAATCTCTA+TGG | - | chr1_2:68954183-68954202 | Msa0087930:CDS | 35.0% | |
| ATTCTTTGAGCTCCATGTAT+TGG | - | chr1_2:68954300-68954319 | Msa0087930:CDS | 35.0% | |
| TTCTTTGAGCTCCATGTATT+GGG | - | chr1_2:68954301-68954320 | Msa0087930:CDS | 35.0% | |
| !! | CTTGGCATCTGATTTGGAAT+AGG | + | chr1_2:68954003-68954022 | None:intergenic | 40.0% |
| CTATTCCAAATCAGATGCCA+AGG | - | chr1_2:68954001-68954020 | Msa0087930:CDS | 40.0% | |
| TCTTTGAGCTCCATGTATTG+GGG | - | chr1_2:68954302-68954321 | Msa0087930:CDS | 40.0% | |
| CACTTGTTTCACAACCATAG+TGG | + | chr1_2:68954339-68954358 | None:intergenic | 40.0% | |
| CACTATGGTTGTGAAACAAG+TGG | - | chr1_2:68954337-68954356 | Msa0087930:CDS | 40.0% | |
| ! | AGTGGTTGATGCTGCATTTA+AGG | - | chr1_2:68954355-68954374 | Msa0087930:CDS | 40.0% |
| !! | TCGAGTTGATGTGGAAAATG+TGG | - | chr1_2:68954406-68954425 | Msa0087930:CDS | 40.0% |
| TGGAAAATGTGGCTTCACAA+AGG | - | chr1_2:68954417-68954436 | Msa0087930:CDS | 40.0% | |
| GGAAAATGTGGCTTCACAAA+GGG | - | chr1_2:68954418-68954437 | Msa0087930:CDS | 40.0% | |
| CTCTTCCTTGGCATCTGATT+TGG | + | chr1_2:68954009-68954028 | None:intergenic | 45.0% | |
| !! | GTGGACTAGTGATGCAAAAG+TGG | - | chr1_2:68954100-68954119 | Msa0087930:CDS | 45.0% |
| ! | AAGTGGCCAAGTATTGCACT+TGG | - | chr1_2:68954117-68954136 | Msa0087930:CDS | 45.0% |
| ! | AGTGGCCAAGTATTGCACTT+GGG | - | chr1_2:68954118-68954137 | Msa0087930:CDS | 45.0% |
| AGGTTCCCAAGTGCAATACT+TGG | + | chr1_2:68954126-68954145 | None:intergenic | 45.0% | |
| TACCGAGTTCCAGGTGTATT+CGG | - | chr1_2:68954244-68954263 | Msa0087930:CDS | 45.0% | |
| ATCCGAATACACCTGGAACT+CGG | + | chr1_2:68954249-68954268 | None:intergenic | 45.0% | |
| AGGTGTATTCGGATAGATGC+CGG | - | chr1_2:68954255-68954274 | Msa0087930:CDS | 45.0% | |
| ! | CAAGTTGAGCAGATTTGTCC+CGG | + | chr1_2:68954277-68954296 | None:intergenic | 45.0% |
| AATCCCTTTGCCCCAATACA+TGG | + | chr1_2:68954315-68954334 | None:intergenic | 45.0% | |
| AGGAGTTTCCATACTTGGAG+AGG | - | chr1_2:68954375-68954394 | Msa0087930:CDS | 45.0% | |
| !! | TTCACAAAGGGTGTTGGAGA+AGG | - | chr1_2:68954430-68954449 | Msa0087930:CDS | 45.0% |
| AATTGGGTGTACCGAGTTCC+AGG | - | chr1_2:68954235-68954254 | Msa0087930:CDS | 50.0% | |
| GGCATCTATCCGAATACACC+TGG | + | chr1_2:68954256-68954275 | None:intergenic | 50.0% | |
| GGTGTATTCGGATAGATGCC+GGG | - | chr1_2:68954256-68954275 | Msa0087930:CDS | 50.0% | |
| CGGGACAAATCTGCTCAACT+TGG | - | chr1_2:68954275-68954294 | Msa0087930:CDS | 50.0% | |
| AGCTCCATGTATTGGGGCAA+AGG | - | chr1_2:68954308-68954327 | Msa0087930:CDS | 50.0% | |
| GCTCCATGTATTGGGGCAAA+GGG | - | chr1_2:68954309-68954328 | Msa0087930:CDS | 50.0% | |
| TCCATACTTGGAGAGGCTTG+AGG | - | chr1_2:68954382-68954401 | Msa0087930:CDS | 50.0% | |
| ! | TGTGGCTTCACAAAGGGTGT+TGG | - | chr1_2:68954424-68954443 | Msa0087930:CDS | 50.0% |
| !! | TGGAGAAGGCTGGCTTTGAA+AGG | - | chr1_2:68954444-68954463 | Msa0087930:CDS | 50.0% |
| !! | GGAGAAGGCTGGCTTTGAAA+GGG | - | chr1_2:68954445-68954464 | Msa0087930:CDS | 50.0% |
| !! | AAGGCTGGCTTTGAAAGGGA+AGG | - | chr1_2:68954449-68954468 | Msa0087930:CDS | 50.0% |
| TGCCTTCGCGATCGAGCAAT+TGG | - | chr1_2:68954218-68954237 | Msa0087930:CDS | 55.0% | |
| GCCTTCGCGATCGAGCAATT+GGG | - | chr1_2:68954219-68954238 | Msa0087930:CDS | 55.0% | |
| ACCCAATTGCTCGATCGCGA+AGG | + | chr1_2:68954223-68954242 | None:intergenic | 55.0% | |
| GGGCAAAGGGATTGCCACTA+TGG | - | chr1_2:68954322-68954341 | Msa0087930:CDS | 55.0% | |
| GCCTCAAGCCTCTCCAAGTA+TGG | + | chr1_2:68954386-68954405 | None:intergenic | 55.0% | |
| !! | GCTTGAGGCTCGAGTTGATG+TGG | - | chr1_2:68954397-68954416 | Msa0087930:CDS | 55.0% |
| !! | CAAAGGGTGTTGGAGAAGGC+TGG | - | chr1_2:68954434-68954453 | Msa0087930:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 68953981 | 68954556 | 68953981 | ID=Msa0087930;Name=Msa0087930 |
| chr1_2 | mRNA | 68953981 | 68954556 | 68953981 | ID=Msa0087930-mRNA-1;Parent=Msa0087930;Name=Msa0087930-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|191 |
| chr1_2 | exon | 68953981 | 68954556 | 68953981 | ID=Msa0087930-mRNA-1:exon:23647;Parent=Msa0087930-mRNA-1 |
| chr1_2 | CDS | 68953981 | 68954556 | 68953981 | ID=Msa0087930-mRNA-1:cds;Parent=Msa0087930-mRNA-1 |
| Gene Sequence |
| Protein sequence |
