AlfalfaGEDB | Msa0087930

M. sativa cultivar zhongmu-4 / Msa0087930

KEGG: -
GO: -
PFAM: PF13302.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087930	sp\|P05332\|YP20_BACLI	34.503	171	103	3	24	188	8	175	4.39e-25	99.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087930	A0A072VP71	96.316	190	7	0	2	191	1	190	1.85e-131	375

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TFs/TRs:

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Gene ID	Type	Classification
Msa0087930	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087930	MtrunA17_Chr1g0204971	96.316	190	7	0	2	191	1	190	3.56e-135	375
Msa0087930	MtrunA17_Chr1g0204951	83.158	190	32	0	2	191	1	190	3.29e-116	328
Msa0087930	MtrunA17_Chr5g0394621	61.458	192	70	2	2	191	1	190	9.72e-82	240
Msa0087930	MtrunA17_Chr5g0394631	60.656	183	68	2	2	182	1	181	5.33e-73	225
Msa0087930	MtrunA17_Chr5g0394631	53.299	197	84	5	1	191	207	401	5.10e-64	202
Msa0087930	MtrunA17_Chr7g0273661	48.077	156	76	2	20	173	4	156	1.10e-44	145
Msa0087930	MtrunA17_Chr7g0223361	39.181	171	102	2	18	188	2	170	2.64e-38	129
Msa0087930	MtrunA17_Chr7g0223351	40.964	166	95	3	23	188	7	169	7.57e-37	126

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087930	AT2G32030.1	45.503	189	98	3	1	188	1	185	4.42e-55	173
Msa0087930	AT2G32020.1	51.497	167	76	3	23	188	18	180	1.08e-54	172
Msa0087930	AT3G22560.1	39.655	174	98	2	19	188	2	172	8.37e-36	124

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Find 51 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACAGAATACTCTTCCT+TGG	0.516633	1_2:+68954497	None:intergenic
AAGGCTGGCTTTGAAAGGGA+AGG	0.642488	1_2:-68954066	Msa0087930:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.595225	1_2:-68954398	Msa0087930:CDS
AATCCCTTTGCCCCAATACA+TGG	0.496567	1_2:+68954203	None:intergenic
AATTGGGTGTACCGAGTTCC+AGG	0.375481	1_2:-68954280	Msa0087930:CDS
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1_2:-68954332	Msa0087930:CDS
ACCCAATTGCTCGATCGCGA+AGG	0.639639	1_2:+68954295	None:intergenic
AGAAAATATTTGTTCATCAA+GGG	0.502192	1_2:-68954036	Msa0087930:CDS
AGCTCCATGTATTGGGGCAA+AGG	0.494926	1_2:-68954207	Msa0087930:CDS
AGGAGTTTCCATACTTGGAG+AGG	0.574247	1_2:-68954140	Msa0087930:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAGATCAGAAAGACTAAT+TGG	+	chr1_2:68954068-68954087	None:intergenic	30.0%
ATTTAAGGAGTTTCCATACT+TGG	-	chr1_2:68954370-68954389	Msa0087930:CDS	30.0%
AACAACAGAATACTCTTCCT+TGG	+	chr1_2:68954021-68954040	None:intergenic	35.0%
TAATTGGTCGAAGAGAGATT+TGG	+	chr1_2:68954052-68954071	None:intergenic	35.0%
ACATAGCAAGCAAATCTCTA+TGG	-	chr1_2:68954183-68954202	Msa0087930:CDS	35.0%
ATTCTTTGAGCTCCATGTAT+TGG	-	chr1_2:68954300-68954319	Msa0087930:CDS	35.0%
TTCTTTGAGCTCCATGTATT+GGG	-	chr1_2:68954301-68954320	Msa0087930:CDS	35.0%
CTATTCCAAATCAGATGCCA+AGG	-	chr1_2:68954001-68954020	Msa0087930:CDS	40.0%
TCTTTGAGCTCCATGTATTG+GGG	-	chr1_2:68954302-68954321	Msa0087930:CDS	40.0%
CACTTGTTTCACAACCATAG+TGG	+	chr1_2:68954339-68954358	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	68953981	68954556	68953981	ID=Msa0087930;Name=Msa0087930
chr1_2	mRNA	68953981	68954556	68953981	ID=Msa0087930-mRNA-1;Parent=Msa0087930;Name=Msa0087930-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|191
chr1_2	exon	68953981	68954556	68953981	ID=Msa0087930-mRNA-1:exon:23647;Parent=Msa0087930-mRNA-1
chr1_2	CDS	68953981	68954556	68953981	ID=Msa0087930-mRNA-1:cds;Parent=Msa0087930-mRNA-1

Gene Sequence

>Msa0087930

ATGATGGAAGAAACTGCTACCTATTCCAAATCAGATGCCAAGGAAGAGTATTCTGTTGTTGACTTGAGCCAAATCTCTCTTCGACCAATTAGTCTTTCTGATCTTGATGATGTTATGTTGTGGACTAGTGATGCAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAAAAGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATCGAGCAATTGGGTGTACCGAGTTCCAGGTGTATTCGGATAGATGCCGGGACAAATCTGCTCAACTTGGCTATTCTTTGAGCTCCATGTATTGGGGCAAAGGGATTGCCACTATGGTTGTGAAACAAGTGGTTGATGCTGCATTTAAGGAGTTTCCATACTTGGAGAGGCTTGAGGCTCGAGTTGATGTGGAAAATGTGGCTTCACAAAGGGTGTTGGAGAAGGCTGGCTTTGAAAGGGAAGGTATTCTCAGAAAATATTTGTTCATCAAGGGAAAAAGTATAGATATTGTTATGTTCAGTATTCTCTCAAGTGAAGTTAAACTTTGA

Protein sequence

>Msa0087930

MMEETATYSKSDAKEEYSVVDLSQISLRPISLSDLDDVMLWTSDAKVAKYCTWEPYTNKEKGIDFIQNIASKSLWFRAICLRDRAIGCTEFQVYSDRCRDKSAQLGYSLSSMYWGKGIATMVVKQVVDAAFKEFPYLERLEARVDVENVASQRVLEKAGFEREGILRKYLFIKGKSIDIVMFSILSSEVKL*