AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0088630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0088630 MtrunA17_Chr2g0277381 98.701 231 3 0 1 231 145 375 5.86e-171 475
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0088630 AT4G06634.3 61.572 229 72 7 1 217 140 364 5.77e-81 247
Msa0088630 AT4G06634.2 61.135 229 73 7 1 217 109 333 8.23e-81 246
Msa0088630 AT4G06634.1 61.135 229 73 7 1 217 147 371 5.08e-80 245

Find 53 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTTATCGGCACATCTAT+TGG 0.312836 1_2:+69717309 None:intergenic
GCGTATCTCTTCCCACAATC+TGG 0.321481 1_2:+69718971 None:intergenic
TTACGCTGAGCAACTTTAGA+AGG 0.345270 1_2:+69716970 None:intergenic
CTAAAGTTGCTCAGCGTAAA+GGG 0.392561 1_2:-69716966 Msa0088630:CDS
GGAGGAGTTGCATACTTTAT+CGG 0.401475 1_2:+69717297 None:intergenic
TGACATAGTACATTGGTTAT+CGG 0.402772 1_2:-69716665 Msa0088630:three_prime_UTR
CCTTCATAAGGACATGCATA+TGG 0.405323 1_2:+69717207 None:intergenic
CTCTTCCCACAATCTGGGTA+TGG 0.407430 1_2:+69718977 None:intergenic
GTTTCTTCTTTCACTGGCCA+AGG 0.432210 1_2:+69716904 None:intergenic
GCAAGTGATCACGATGCTTA+TGG 0.440454 1_2:-69717057 Msa0088630:CDS
TCTAAAGTTGCTCAGCGTAA+AGG 0.457185 1_2:-69716967 Msa0088630:CDS
TCGTAAGTTTCTTCTTTCAC+TGG 0.459248 1_2:+69716898 None:intergenic
CAATATGCAGGCCTTCTCCT+TGG 0.459806 1_2:-69719056 None:intergenic
CATTGCTTCCCATCATGAAA+AGG 0.475165 1_2:-69718924 Msa0088630:intron
TAATAATGCTGAAAATGAAA+TGG 0.485384 1_2:-69717085 Msa0088630:CDS
AGCTTAAACTGCATTTAAAG+AGG 0.494339 1_2:-69717155 Msa0088630:CDS
ATATTAAAAGTGGCAGGGGT+TGG 0.494716 1_2:+69716937 None:intergenic
TTAGCCATATTAAAAGTGGC+AGG 0.498748 1_2:+69716931 None:intergenic
CCATATGCATGTCCTTATGA+AGG 0.500362 1_2:-69717207 Msa0088630:CDS
TCCTTATGAAGGGTGTGAAA+AGG 0.501607 1_2:-69717196 Msa0088630:CDS
TGAAAATGCTATGCATGCTC+TGG 0.502807 1_2:-69717109 Msa0088630:CDS
GAAGATGGTTGGAGATATGC+TGG 0.512941 1_2:-69716832 Msa0088630:CDS
CGTATCTCTTCCCACAATCT+GGG 0.520117 1_2:+69718972 None:intergenic
CTTCATAAGGACATGCATAT+GGG 0.522116 1_2:+69717208 None:intergenic
GTAAAAGTCAGAAGCAAAGC+AGG 0.525445 1_2:-69717017 Msa0088630:CDS
TTCTGGAGCGACATACGGTT+CGG 0.536084 1_2:-69717244 Msa0088630:CDS
CAGCGAAGAAACTGAGGAGG+AGG 0.538145 1_2:-69716869 Msa0088630:CDS
AGACCTTCTGGAGCGACATA+CGG 0.541337 1_2:-69717249 Msa0088630:CDS
GTGTCTTCATGTGAGACCTC+AGG 0.542819 1_2:+69719026 None:intergenic
ATTAATATAAATTCATCCTA+CGG 0.545288 1_2:+69716628 None:intergenic
TCTTCCCACAATCTGGGTAT+GGG 0.550401 1_2:+69718978 None:intergenic
CGCTGAGCAACTTTAGAAGG+AGG 0.554525 1_2:+69716973 None:intergenic
TATGCCCATACCCAGATTGT+GGG 0.558178 1_2:-69718982 Msa0088630:CDS
GTGACAATGTTGAAGATGGT+TGG 0.563304 1_2:-69716843 Msa0088630:CDS
GCTTAAACTGCATTTAAAGA+GGG 0.566396 1_2:-69717154 Msa0088630:CDS
AGCCATATTAAAAGTGGCAG+GGG 0.567185 1_2:+69716933 None:intergenic
CATTTAAAGAGGGAGCATCC+TGG 0.569801 1_2:-69717144 Msa0088630:CDS
ATATGCCCATACCCAGATTG+TGG 0.578913 1_2:-69718983 Msa0088630:CDS
AGACCTCAGGTTAAAATCCA+AGG 0.579184 1_2:+69719039 None:intergenic
TAGCCATATTAAAAGTGGCA+GGG 0.588156 1_2:+69716932 None:intergenic
CAGGTTAAAATCCAAGGAGA+AGG 0.604105 1_2:+69719045 None:intergenic
GAACCGTATGTCGCTCCAGA+AGG 0.605176 1_2:+69717246 None:intergenic
CATATGCATGTCCTTATGAA+GGG 0.608937 1_2:-69717206 Msa0088630:CDS
ATAACCAATGTACTATGTCA+AGG 0.609060 1_2:+69716668 None:intergenic
AAACAACGAAGATGATGATG+AGG 0.613095 1_2:-69716809 Msa0088630:CDS
GATCGTGACAATGTTGAAGA+TGG 0.616587 1_2:-69716847 Msa0088630:CDS
TATGGTGGAAAACGTTCCAA+TGG 0.620791 1_2:-69717039 Msa0088630:CDS
ACATGCATATGGGCGATCAG+AGG 0.622960 1_2:+69717218 None:intergenic
AGTGATCACGATGCTTATGG+TGG 0.628298 1_2:-69717054 Msa0088630:CDS
TGAAGACAGCGAAGAAACTG+AGG 0.661243 1_2:-69716875 Msa0088630:CDS
AGACAGCGAAGAAACTGAGG+AGG 0.668007 1_2:-69716872 Msa0088630:CDS
GGAAGAATGAATGATACCGT+AGG 0.673395 1_2:-69716644 Msa0088630:three_prime_UTR
AATCGCGGTAGTTTCTGACG+CGG 0.713483 1_2:-69716563 Msa0088630:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTATATTCTATTCTAAA+TGG - chr1_2:69716694-69716713 Msa0088630:three_prime_UTR 10.0%
!! CATATAAAGATAAAAAAAAT+TGG + chr1_2:69716973-69716992 None:intergenic 10.0%
!!! AATATATAAATTTTCAAGTT+CGG - chr1_2:69717691-69717710 Msa0088630:intron 10.0%
!! TTGATTATTATAAATTTCAG+GGG + chr1_2:69716910-69716929 None:intergenic 15.0%
!! CTTGATTATTATAAATTTCA+GGG + chr1_2:69716911-69716930 None:intergenic 15.0%
!! TTTATAATAATCAAGCAAAT+TGG - chr1_2:69716916-69716935 Msa0088630:CDS 15.0%
!! AAGAAAATCTTTAAACTATA+TGG - chr1_2:69717046-69717065 Msa0088630:CDS 15.0%
!!! ATATATAAATTTTCAAGTTC+GGG - chr1_2:69717692-69717711 Msa0088630:intron 15.0%
!!! AATTGAAGTTTTATTAATGT+CGG - chr1_2:69717832-69717851 Msa0088630:intron 15.0%
!!! TATTTTAATACTATTTTGCA+AGG - chr1_2:69718023-69718042 Msa0088630:intron 15.0%
!! AAGAAAAAATCTAATATTGT+AGG + chr1_2:69718840-69718859 None:intergenic 15.0%
!! ATTAATATAAATTCATCCTA+CGG + chr1_2:69718936-69718955 None:intergenic 15.0%
!!! TTGAAATTTTGTTAATGTCA+GGG + chr1_2:69716769-69716788 None:intergenic 20.0%
!!! ATTGAAATTTTGTTAATGTC+AGG + chr1_2:69716770-69716789 None:intergenic 20.0%
!! TGATTATTATAAATTTCAGG+GGG + chr1_2:69716909-69716928 None:intergenic 20.0%
!! GCTTGATTATTATAAATTTC+AGG + chr1_2:69716912-69716931 None:intergenic 20.0%
!!! AGAATATTTTTTTTGTCACA+AGG + chr1_2:69716943-69716962 None:intergenic 20.0%
!!! AGAAAGAATAATTTTTATGC+TGG - chr1_2:69717094-69717113 Msa0088630:CDS 20.0%
!! TGTTATACCTTTCAAATATT+TGG - chr1_2:69717174-69717193 Msa0088630:CDS 20.0%
!! GTTATACCTTTCAAATATTT+GGG - chr1_2:69717175-69717194 Msa0088630:CDS 20.0%
!!! TAAGATTTTGGACTAATAAT+TGG - chr1_2:69717551-69717570 Msa0088630:intron 20.0%
!!! TATATAAATTTTCAAGTTCG+GGG - chr1_2:69717693-69717712 Msa0088630:intron 20.0%
!!! ATTGAAGTTTTATTAATGTC+GGG - chr1_2:69717833-69717852 Msa0088630:intron 20.0%
!! TAATAATGCTGAAAATGAAA+TGG - chr1_2:69718476-69718495 Msa0088630:intron 20.0%
!!! TTTTTTAGCCATATTAAAAG+TGG + chr1_2:69718637-69718656 None:intergenic 20.0%
!! AAAAAATCTAATATTGTAGG+AGG + chr1_2:69718837-69718856 None:intergenic 20.0%
! CTATTCTAAATGGAGTAAAT+AGG - chr1_2:69716704-69716723 Msa0088630:three_prime_UTR 25.0%
! ATTGACGAAACTTACAATTT+CGG + chr1_2:69717862-69717881 None:intergenic 25.0%
!! TGAAATTTTGTTAATGTCAG+GGG + chr1_2:69716768-69716787 None:intergenic 25.0%
! TAGGTTTATCTTTCAAAATG+TGG + chr1_2:69717204-69717223 None:intergenic 25.0%
! TCATTAAAGTAACTGATCTT+AGG + chr1_2:69717223-69717242 None:intergenic 25.0%
!! GGAATTTTTCTGTCTATAAT+GGG - chr1_2:69717283-69717302 Msa0088630:CDS 25.0%
!! TTTCTTTTTGCAATTACCTA+TGG - chr1_2:69717350-69717369 Msa0088630:intron 25.0%
! TTTCCAAAATTCATATGAAG+TGG - chr1_2:69717406-69717425 Msa0088630:intron 25.0%
!! TATCCACTTCATATGAATTT+TGG + chr1_2:69717412-69717431 None:intergenic 25.0%
!! TTGGTGCAAATATAAGATTT+TGG - chr1_2:69717539-69717558 Msa0088630:intron 25.0%
!! TTGGTGCAAATATAAGATTT+TGG - chr1_2:69717539-69717558 Msa0088630:intron 25.0%
!! ACTTGTTGATCATAATCATT+GGG - chr1_2:69717656-69717675 Msa0088630:intron 25.0%
!!! ATATAAATTTTCAAGTTCGG+GGG - chr1_2:69717694-69717713 Msa0088630:intron 25.0%
! TAACAGAAAAATTTGACGAA+GGG - chr1_2:69717773-69717792 Msa0088630:intron 25.0%
! ATTAACGTTGTACAATTTCA+GGG - chr1_2:69717805-69717824 Msa0088630:intron 25.0%
!!! TTGAAGTTTTATTAATGTCG+GGG - chr1_2:69717834-69717853 Msa0088630:intron 25.0%
!! CACATTTAGAATGAGTAAAT+AGG + chr1_2:69717899-69717918 None:intergenic 25.0%
!!! ATGATTTTAATATGTGCACT+TGG - chr1_2:69717959-69717978 Msa0088630:intron 25.0%
!!! AATTTTTGATTGAGCACTTT+TGG - chr1_2:69718150-69718169 Msa0088630:intron 25.0%
! TGTCAATCAAAAATTGTTTG+AGG + chr1_2:69718208-69718227 None:intergenic 25.0%
!! TAGCATTTTCATCATTTATG+TGG + chr1_2:69718443-69718462 None:intergenic 25.0%
! TTAATATGGCTAAAAAACCT+TGG - chr1_2:69718640-69718659 Msa0088630:intron 25.0%
! TTAACCAACCTTTTCATGAT+GGG + chr1_2:69716648-69716667 None:intergenic 30.0%
! ATTAACCAACCTTTTCATGA+TGG + chr1_2:69716649-69716668 None:intergenic 30.0%
TTGACGAAACTTACAATTTC+GGG + chr1_2:69717863-69717882 None:intergenic 30.0%
! AAATTTTGTTAATGTCAGGG+GGG + chr1_2:69716766-69716785 None:intergenic 30.0%
! GAAATTTTGTTAATGTCAGG+GGG + chr1_2:69716767-69716786 None:intergenic 30.0%
ATTAACGTTGTGCAATTTCA+GGG + chr1_2:69716798-69716817 None:intergenic 30.0%
TAACGGAAAAATTTGACAGA+GGG + chr1_2:69716830-69716849 None:intergenic 30.0%
! GATATCTGAATTTTTCGCAA+AGG - chr1_2:69716998-69717017 Msa0088630:CDS 30.0%
! TTTTTCGCAAAGGATCAAAT+AGG - chr1_2:69717008-69717027 Msa0088630:CDS 30.0%
!! TCATGTAATTAGCTTTAACC+TGG - chr1_2:69717150-69717169 Msa0088630:CDS 30.0%
!! TTAAAGTTAAGCTTGCTACT+TGG - chr1_2:69717257-69717276 Msa0088630:CDS 30.0%
! GGGAATTTTTCTGTCTATAA+TGG - chr1_2:69717282-69717301 Msa0088630:CDS 30.0%
!! CTCATCAAGTTTGTTATTCA+TGG - chr1_2:69717316-69717335 Msa0088630:CDS 30.0%
!!! CTGCTATTTTACTTCTTTGT+TGG - chr1_2:69717451-69717470 Msa0088630:intron 30.0%
! ACTCTAAAGATCATTGCTAT+TGG - chr1_2:69717520-69717539 Msa0088630:intron 30.0%
! TTTTGGACTAATAATTGGCT+TGG - chr1_2:69717556-69717575 Msa0088630:intron 30.0%
! TTTGGACTAATAATTGGCTT+GGG - chr1_2:69717557-69717576 Msa0088630:intron 30.0%
! CACTTGTTGATCATAATCAT+TGG - chr1_2:69717655-69717674 Msa0088630:intron 30.0%
CTTGTTGATCATAATCATTG+GGG - chr1_2:69717657-69717676 Msa0088630:intron 30.0%
!!! TATAAATTTTCAAGTTCGGG+GGG - chr1_2:69717695-69717714 Msa0088630:intron 30.0%
CTAACAGAAAAATTTGACGA+AGG - chr1_2:69717772-69717791 Msa0088630:intron 30.0%
AACAGAAAAATTTGACGAAG+GGG - chr1_2:69717774-69717793 Msa0088630:intron 30.0%
GATTAACGTTGTACAATTTC+AGG - chr1_2:69717804-69717823 Msa0088630:intron 30.0%
TTAACGTTGTACAATTTCAG+GGG - chr1_2:69717806-69717825 Msa0088630:intron 30.0%
!!! TGAAGTTTTATTAATGTCGG+GGG - chr1_2:69717835-69717854 Msa0088630:intron 30.0%
TGACGAAACTTACAATTTCA+AGG - chr1_2:69717864-69717883 Msa0088630:intron 30.0%
GACGAAACTTACAATTTCAA+GGG - chr1_2:69717865-69717884 Msa0088630:intron 30.0%
ACGAAACTTACAATTTCAAG+GGG - chr1_2:69717866-69717885 Msa0088630:intron 30.0%
AAAATCATACTTCATGAGAC+AGG + chr1_2:69717947-69717966 None:intergenic 30.0%
GCTCAATCAAAAATTGTTTG+AGG + chr1_2:69718145-69718164 None:intergenic 30.0%
!! GTTTTTGTTTGCTTTTCTGA+AGG + chr1_2:69718288-69718307 None:intergenic 30.0%
AGCTTAAACTGCATTTAAAG+AGG - chr1_2:69718406-69718425 Msa0088630:intron 30.0%
GCTTAAACTGCATTTAAAGA+GGG - chr1_2:69718407-69718426 Msa0088630:intron 30.0%
! TTTTCCTTGACATAGTACAT+TGG - chr1_2:69718889-69718908 Msa0088630:intron 30.0%
ATAACCAATGTACTATGTCA+AGG + chr1_2:69718896-69718915 None:intergenic 30.0%
! TGACATAGTACATTGGTTAT+CGG - chr1_2:69718896-69718915 Msa0088630:intron 30.0%
TAGATCACAGTTACACAAAC+AGG + chr1_2:69716676-69716695 None:intergenic 35.0%
TGACGAAACTTACAATTTCG+GGG + chr1_2:69716739-69716758 None:intergenic 35.0%
TTAACGTTGTGCAATTTCAG+GGG + chr1_2:69716797-69716816 None:intergenic 35.0%
GATTAACGTTGTGCAATTTC+AGG + chr1_2:69716799-69716818 None:intergenic 35.0%
AACGGAAAAATTTGACAGAG+GGG + chr1_2:69716829-69716848 None:intergenic 35.0%
CTAACGGAAAAATTTGACAG+AGG + chr1_2:69716831-69716850 None:intergenic 35.0%
! CAAAACGTCATGTTGACTAA+CGG + chr1_2:69716847-69716866 None:intergenic 35.0%
ACATAAGTGCAAAACTTCAG+GGG + chr1_2:69717726-69717745 None:intergenic 35.0%
CACATAAGTGCAAAACTTCA+GGG + chr1_2:69717725-69717744 None:intergenic 35.0%
! ATTTGAAAGGTATAACAGCC+AGG + chr1_2:69717171-69717190 None:intergenic 35.0%
TGGACTCCCAAATATTTGAA+AGG + chr1_2:69717184-69717203 None:intergenic 35.0%
TTTCGAAGCAACTTGATCAA+TGG - chr1_2:69717383-69717402 Msa0088630:intron 35.0%
!! ACTAATAATTGGCTTGGGAT+TGG - chr1_2:69717562-69717581 Msa0088630:intron 35.0%
CACATAAGTGCAAAACTTCA+GGG - chr1_2:69717725-69717744 Msa0088630:intron 35.0%
ACATAAGTGCAAAACTTCAG+GGG - chr1_2:69717726-69717745 Msa0088630:intron 35.0%
ACAGAAAAATTTGACGAAGG+GGG - chr1_2:69717775-69717794 Msa0088630:intron 35.0%
TAACGTTGTACAATTTCAGG+GGG - chr1_2:69717807-69717826 Msa0088630:intron 35.0%
!!! GAAGTTTTATTAATGTCGGG+GGG - chr1_2:69717836-69717855 Msa0088630:intron 35.0%
!!! AAGTTTTATTAATGTCGGGG+GGG - chr1_2:69717837-69717856 Msa0088630:intron 35.0%
CGAAACTTACAATTTCAAGG+GGG - chr1_2:69717867-69717886 Msa0088630:intron 35.0%
GTGTTCACAATCACAATAGA+TGG - chr1_2:69717916-69717935 Msa0088630:intron 35.0%
TACTTTATCGGCACATCTAT+TGG + chr1_2:69718255-69718274 None:intergenic 35.0%
!! TTTGTTTGCTTTTCTGAAGG+AGG + chr1_2:69718285-69718304 None:intergenic 35.0%
CTTCATAAGGACATGCATAT+GGG + chr1_2:69718356-69718375 None:intergenic 35.0%
CATATGCATGTCCTTATGAA+GGG - chr1_2:69718355-69718374 Msa0088630:intron 35.0%
! TTTTCATCATTTATGTGGCC+AGG + chr1_2:69718438-69718457 None:intergenic 35.0%
! TTGCTTCTGACTTTTACCAT+TGG + chr1_2:69718541-69718560 None:intergenic 35.0%
TAGCCATATTAAAAGTGGCA+GGG + chr1_2:69718632-69718651 None:intergenic 35.0%
TTAGCCATATTAAAAGTGGC+AGG + chr1_2:69718633-69718652 None:intergenic 35.0%
TCGTAAGTTTCTTCTTTCAC+TGG + chr1_2:69718666-69718685 None:intergenic 35.0%
! AAACAACGAAGATGATGATG+AGG - chr1_2:69718752-69718771 Msa0088630:intron 35.0%
CAGGTTAAAATCCAAGGAGA+AGG + chr1_2:69716519-69716538 None:intergenic 40.0%
!! TCTCCTTGGATTTTAACCTG+AGG - chr1_2:69716519-69716538 Msa0088630:three_prime_UTR 40.0%
AGACCTCAGGTTAAAATCCA+AGG + chr1_2:69716525-69716544 None:intergenic 40.0%
CATTGCTTCCCATCATGAAA+AGG - chr1_2:69716637-69716656 Msa0088630:three_prime_UTR 40.0%
ACGAAACTTACAATTTCGGG+GGG + chr1_2:69716737-69716756 None:intergenic 40.0%
GACGAAACTTACAATTTCGG+GGG + chr1_2:69716738-69716757 None:intergenic 40.0%
TAACGTTGTGCAATTTCAGG+GGG + chr1_2:69716796-69716815 None:intergenic 40.0%
ACGGAAAAATTTGACAGAGG+GGG + chr1_2:69716828-69716847 None:intergenic 40.0%
ATAAGTGCAAAACTTCAGGG+GGG + chr1_2:69717728-69717747 None:intergenic 40.0%
CATAAGTGCAAAACTTCAGG+GGG + chr1_2:69717727-69717746 None:intergenic 40.0%
GCACATAAGTGCAAAACTTC+AGG + chr1_2:69717724-69717743 None:intergenic 40.0%
!! AGTTAAGCTTGCTACTTGGA+AGG - chr1_2:69717261-69717280 Msa0088630:CDS 40.0%
!! GTTAAGCTTGCTACTTGGAA+GGG - chr1_2:69717262-69717281 Msa0088630:CDS 40.0%
AGTTGCTTCGAAAGATGTAC+AGG + chr1_2:69717376-69717395 None:intergenic 40.0%
ACAAGTGAGCAACAAATGTG+AGG + chr1_2:69717642-69717661 None:intergenic 40.0%
GCACATAAGTGCAAAACTTC+AGG - chr1_2:69717724-69717743 Msa0088630:intron 40.0%
CATAAGTGCAAAACTTCAGG+GGG - chr1_2:69717727-69717746 Msa0088630:intron 40.0%
AACGTTGTACAATTTCAGGG+GGG - chr1_2:69717808-69717827 Msa0088630:intron 40.0%
CGTCAAATGAAACCTCTAGA+TGG + chr1_2:69717988-69718007 None:intergenic 40.0%
! ATGATGCATGACTGCACTTT+TGG - chr1_2:69718087-69718106 Msa0088630:intron 40.0%
GGAGGAGTTGCATACTTTAT+CGG + chr1_2:69718267-69718286 None:intergenic 40.0%
ACAAAAACTGCTAGACCTTC+TGG - chr1_2:69718300-69718319 Msa0088630:intron 40.0%
CCTTCATAAGGACATGCATA+TGG + chr1_2:69718357-69718376 None:intergenic 40.0%
CCATATGCATGTCCTTATGA+AGG - chr1_2:69718354-69718373 Msa0088630:intron 40.0%
! TCCTTATGAAGGGTGTGAAA+AGG - chr1_2:69718365-69718384 Msa0088630:intron 40.0%
TGAAAATGCTATGCATGCTC+TGG - chr1_2:69718452-69718471 Msa0088630:intron 40.0%
! TATGGTGGAAAACGTTCCAA+TGG - chr1_2:69718522-69718541 Msa0088630:intron 40.0%
GTAAAAGTCAGAAGCAAAGC+AGG - chr1_2:69718544-69718563 Msa0088630:intron 40.0%
!!! GGCATTTTCAAGTTTGGCTT+GGG + chr1_2:69718570-69718589 None:intergenic 40.0%
!!! AGGCATTTTCAAGTTTGGCT+TGG + chr1_2:69718571-69718590 None:intergenic 40.0%
!!! GAAGGAGGCATTTTCAAGTT+TGG + chr1_2:69718576-69718595 None:intergenic 40.0%
TTACGCTGAGCAACTTTAGA+AGG + chr1_2:69718594-69718613 None:intergenic 40.0%
! TCTAAAGTTGCTCAGCGTAA+AGG - chr1_2:69718594-69718613 Msa0088630:intron 40.0%
CTAAAGTTGCTCAGCGTAAA+GGG - chr1_2:69718595-69718614 Msa0088630:intron 40.0%
ATATTAAAAGTGGCAGGGGT+TGG + chr1_2:69718627-69718646 None:intergenic 40.0%
!!! AACCCCTGCCACTTTTAATA+TGG - chr1_2:69718626-69718645 Msa0088630:intron 40.0%
AGCCATATTAAAAGTGGCAG+GGG + chr1_2:69718631-69718650 None:intergenic 40.0%
GATCGTGACAATGTTGAAGA+TGG - chr1_2:69718714-69718733 Msa0088630:intron 40.0%
GTGACAATGTTGAAGATGGT+TGG - chr1_2:69718718-69718737 Msa0088630:intron 40.0%
GGAAGAATGAATGATACCGT+AGG - chr1_2:69718917-69718936 Msa0088630:intron 40.0%
! ATATTTTCTGCTGCGAATCG+CGG - chr1_2:69718983-69719002 Msa0088630:CDS 40.0%
ATATGCCCATACCCAGATTG+TGG - chr1_2:69716578-69716597 Msa0088630:three_prime_UTR 45.0%
TATGCCCATACCCAGATTGT+GGG - chr1_2:69716579-69716598 Msa0088630:three_prime_UTR 45.0%
TCTTCCCACAATCTGGGTAT+GGG + chr1_2:69716586-69716605 None:intergenic 45.0%
CGTATCTCTTCCCACAATCT+GGG + chr1_2:69716592-69716611 None:intergenic 45.0%
GCTTCCCATCATGAAAAGGT+TGG - chr1_2:69716641-69716660 Msa0088630:three_prime_UTR 45.0%
CGAAACTTACAATTTCGGGG+GGG + chr1_2:69716736-69716755 None:intergenic 45.0%
AACGTTGTGCAATTTCAGGG+GGG + chr1_2:69716795-69716814 None:intergenic 45.0%
! CAAGCATTGCACTTCAGACA+GGG + chr1_2:69717129-69717148 None:intergenic 45.0%
TCGAAAGATGTACAGGCCAT+AGG + chr1_2:69717369-69717388 None:intergenic 45.0%
TGCACTTGGTTTCCATCTAG+AGG - chr1_2:69717973-69717992 Msa0088630:intron 45.0%
TATTGGTGTACTCTGCAGTG+AGG + chr1_2:69718238-69718257 None:intergenic 45.0%
! GCCTTTTCACACCCTTCATA+AGG + chr1_2:69718369-69718388 None:intergenic 45.0%
!! CATTTAAAGAGGGAGCATCC+TGG - chr1_2:69718417-69718436 Msa0088630:intron 45.0%
GCAAGTGATCACGATGCTTA+TGG - chr1_2:69718504-69718523 Msa0088630:intron 45.0%
AGTGATCACGATGCTTATGG+TGG - chr1_2:69718507-69718526 Msa0088630:intron 45.0%
GTTTCTTCTTTCACTGGCCA+AGG + chr1_2:69718660-69718679 None:intergenic 45.0%
TGAAGACAGCGAAGAAACTG+AGG - chr1_2:69718686-69718705 Msa0088630:intron 45.0%
GAAGATGGTTGGAGATATGC+TGG - chr1_2:69718729-69718748 Msa0088630:intron 45.0%
!! ATATAAAGATAAAAAAAATT+GGG + chr1_2:69716972-69716991 None:intergenic 5.0%
GTGTCTTCATGTGAGACCTC+AGG + chr1_2:69716538-69716557 None:intergenic 50.0%
CTCTTCCCACAATCTGGGTA+TGG + chr1_2:69716587-69716606 None:intergenic 50.0%
GCGTATCTCTTCCCACAATC+TGG + chr1_2:69716593-69716612 None:intergenic 50.0%
! GCAAGCATTGCACTTCAGAC+AGG + chr1_2:69717130-69717149 None:intergenic 50.0%
!! AGACCTTCTGGAGCGACATA+CGG - chr1_2:69718312-69718331 Msa0088630:intron 50.0%
!! TTCTGGAGCGACATACGGTT+CGG - chr1_2:69718317-69718336 Msa0088630:intron 50.0%
ACATGCATATGGGCGATCAG+AGG + chr1_2:69718346-69718365 None:intergenic 50.0%
! CGCTGAGCAACTTTAGAAGG+AGG + chr1_2:69718591-69718610 None:intergenic 50.0%
AGACAGCGAAGAAACTGAGG+AGG - chr1_2:69718689-69718708 Msa0088630:intron 50.0%
AATCGCGGTAGTTTCTGACG+CGG - chr1_2:69718998-69719017 Msa0088630:CDS 50.0%
GAACCGTATGTCGCTCCAGA+AGG + chr1_2:69718318-69718337 None:intergenic 55.0%
CAGCGAAGAAACTGAGGAGG+AGG - chr1_2:69718692-69718711 Msa0088630:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 69716512 69719071 69716512 ID=Msa0088630;Name=Msa0088630
chr1_2 mRNA 69716512 69719071 69716512 ID=Msa0088630-mRNA-1;Parent=Msa0088630;Name=Msa0088630-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.5|1|1|1|2|277|231
chr1_2 exon 69716512 69717337 69716512 ID=Msa0088630-mRNA-1:exon:23997;Parent=Msa0088630-mRNA-1
chr1_2 exon 69718925 69719071 69718925 ID=Msa0088630-mRNA-1:exon:23996;Parent=Msa0088630-mRNA-1
chr1_2 CDS 69718925 69719071 69718925 ID=Msa0088630-mRNA-1:cds;Parent=Msa0088630-mRNA-1
chr1_2 CDS 69716789 69717337 69716789 ID=Msa0088630-mRNA-1:cds;Parent=Msa0088630-mRNA-1
chr1_2 three_prime_UTR 69716512 69716788 69716512 ID=Msa0088630-mRNA-1:three_prime_utr;Parent=Msa0088630-mRNA-1
Gene Sequence

>Msa0088630

CAGGCCTTCTCCTTGGATTTTAACCTGAGGTCTCACATGAAGACACATTCACAAGAGAATTATCATATATGCCCATACCCAGATTGTGGGAAGAGATACGCTCATGAATACAAGCTAAAGAATCACATTGCTTCCCATCATGAAAAGAGTACACCAATAGATGTGCCGATAAAGTATGCAACTCCTCCTTCAGAAAAGCAAACAAAAACTGCTAGACCTTCTGGAGCGACATACGGTTCGGCATCCTCTGATCGCCCATATGCATGTCCTTATGAAGGGTGTGAAAAGGCTTACATTCATGAGTACAAGCTTAAACTGCATTTAAAGAGGGAGCATCCTGGCCACATAAATGATGAAAATGCTATGCATGCTCTGGCTAATAATGCTGAAAATGAAATGGATGAAGCAAGTGATCACGATGCTTATGGTGGAAAACGTTCCAATGGTAAAAGTCAGAAGCAAAGCAGGCCCAAGCCAAACTTGAAAATGCCTCCTTCTAAAGTTGCTCAGCGTAAAGGGTCTACTCCAACCCCTGCCACTTTTAATATGGCTAAAAAACCTTGGCCAGTGAAAGAAGAAACTTACGATGAAGACAGCGAAGAAACTGAGGAGGAGGATCGTGACAATGTTGAAGATGGTTGGAGATATGCTGGAAACAACGAAGATGATGATGAGGAAACAGAATATGAAGACTGA
Protein sequence

>Msa0088630

QAFSLDFNLRSHMKTHSQENYHICPYPDCGKRYAHEYKLKNHIASHHEKSTPIDVPIKYATPPSEKQTKTARPSGATYGSASSDRPYACPYEGCEKAYIHEYKLKLHLKREHPGHINDENAMHALANNAENEMDEASDHDAYGGKRSNGKSQKQSRPKPNLKMPPSKVAQRKGSTPTPATFNMAKKPWPVKEETYDEDSEETEEEDRDNVEDGWRYAGNNEDDDEETEYED*