AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0096910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTAAGCATGCACTTC+TGG 0.248003 1_3:+2655326 Msa0096910:CDS
CAATGGTTCTGAGCTAATTA+AGG 0.256935 1_3:+2653911 Msa0096910:CDS
GGTACTGTAAGACAGGATTT+TGG 0.279481 1_3:+2654045 Msa0096910:intron
GGAGCTGATAACGTGGTTCT+AGG 0.360842 1_3:+2654101 Msa0096910:CDS
CCACCAGGGATGGATCTAAA+AGG 0.365878 1_3:+2654364 Msa0096910:CDS
GCTGGTGCTAGTGCTGCTAT+TGG 0.369554 1_3:+2654466 Msa0096910:CDS
CGTGTGTTTGACTGCCCACC+AGG 0.419940 1_3:+2654349 Msa0096910:CDS
GAAGAGATTCAGAATTTCAA+TGG 0.424440 1_3:+2653894 Msa0096910:CDS
AGGTGATGCATCGTATTTGC+AGG 0.428163 1_3:+2654528 Msa0096910:CDS
GTTGGTTCTGCTGCTGATGC+TGG 0.438309 1_3:+2654490 Msa0096910:CDS
TGATGTCGCTCAGAAACCTA+TGG 0.450742 1_3:+2654441 Msa0096910:CDS
GCTAGTGCTGCTATTGGTGT+TGG 0.469075 1_3:+2654472 Msa0096910:CDS
AACACACGACAAACAACAAA+AGG 0.472249 1_3:-2654334 None:intergenic
CAGAGGAGGTACTGTAAGAC+AGG 0.478625 1_3:+2654038 Msa0096910:intron
CAAATGGGTCATACTGGTAC+AGG 0.492656 1_3:-2653941 None:intergenic
ATGGATCTAAAAGGTGAGTT+CGG 0.503657 1_3:+2654373 Msa0096910:CDS
GAATTGTTTCCACCTGGAGT+TGG 0.516842 1_3:+2653816 Msa0096910:CDS
GATGTCGCTCAGAAACCTAT+GGG 0.517207 1_3:+2654442 Msa0096910:CDS
AGTGCATGCTTAAGCTGCTC+CGG 0.528189 1_3:-2655321 None:intergenic
GAAGGGACGAAGCCAACTCC+AGG 0.543255 1_3:-2653828 None:intergenic
CACTCTTGCTAACAAGCCTC+AGG 0.544263 1_3:+2654202 Msa0096910:CDS
GTTGAAGAAGGCGATGCTGC+TGG 0.544550 1_3:+2654409 Msa0096910:CDS
TGGACGCTGTCAAAACCAGC+TGG 0.550147 1_3:+2654165 Msa0096910:CDS
GCTGGTCGTGCTGCTGAGCA+AGG 0.554733 1_3:+2654508 Msa0096910:CDS
AGGTGAGTTCGGAGCCTTGA+CGG 0.561338 1_3:+2654384 Msa0096910:CDS
GCCTTGACGGATGTTGAAGA+AGG 0.563038 1_3:+2654397 Msa0096910:CDS
CCACATGGAAGCTATGATAC+CGG 0.566035 1_3:+2655302 Msa0096910:CDS
GTTTGACTGCCCACCAGGGA+TGG 0.566884 1_3:+2654354 Msa0096910:CDS
GCTGTTCGAGACATTACCGT+TGG 0.568138 1_3:+2654080 Msa0096910:CDS
GCAATGGAATTGTTTCCACC+TGG 0.583138 1_3:+2653810 None:intergenic
GCCTTCTTCAACATCCGTCA+AGG 0.599602 1_3:-2654398 None:intergenic
GTACTCGTACATAGTCCAGC+TGG 0.603542 1_3:-2654180 None:intergenic
CTAACAAGGAGCTCATCAGA+AGG 0.607644 1_3:-2653846 None:intergenic
TAACAAGGAGCTCATCAGAA+GGG 0.608778 1_3:-2653845 None:intergenic
TGACTGTGTTGTCTTCCACA+TGG 0.610846 1_3:+2655287 Msa0096910:CDS
CTATTTAGGAAATTCACCGA+TGG 0.613156 1_3:+2654145 Msa0096910:CDS
TGGCAAGATAGTAGCTAACA+AGG 0.620882 1_3:-2653860 None:intergenic
AACCACGTTATCAGCTCCAA+CGG 0.626095 1_3:-2654096 None:intergenic
TACCGTTGGAGCTGATAACG+TGG 0.651576 1_3:+2654094 Msa0096910:CDS
GGGACGAAGCCAACTCCAGG+TGG 0.660104 1_3:-2653825 None:intergenic
ATGTCGCTCAGAAACCTATG+GGG 0.679776 1_3:+2654443 Msa0096910:CDS
CCGGTATCATAGCTTCCATG+TGG 0.697172 1_3:-2655302 None:intergenic
GTGTGTTTGACTGCCCACCA+GGG 0.714218 1_3:+2654350 Msa0096910:CDS
TGTCGCTCAGAAACCTATGG+GGG 0.722313 1_3:+2654444 Msa0096910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGAAATTTTAAAGAAT+CGG - chr1_3:2654943-2654962 None:intergenic 10.0%
!!! TTTTTGAAATTTTAAAGAAT+CGG - chr1_3:2654943-2654962 None:intergenic 10.0%
!! TCAATGATTAATTAATGTAT+TGG + chr1_3:2654270-2654289 Msa0096910:intron 15.0%
!! TTATATATAAATAATTGTGC+AGG + chr1_3:2654309-2654328 Msa0096910:intron 15.0%
!! GATAAAATTTATCAAAACTT+TGG + chr1_3:2654792-2654811 Msa0096910:intron 15.0%
!! TCAATGATTAATTAATGTAT+TGG + chr1_3:2654270-2654289 Msa0096910:intron 15.0%
!! TTATATATAAATAATTGTGC+AGG + chr1_3:2654309-2654328 Msa0096910:intron 15.0%
!! GATAAAATTTATCAAAACTT+TGG + chr1_3:2654792-2654811 Msa0096910:intron 15.0%
!! TGATGAAATAAAGAACAAAA+GGG - chr1_3:2654253-2654272 None:intergenic 20.0%
!! TTGATGAAATAAAGAACAAA+AGG - chr1_3:2654254-2654273 None:intergenic 20.0%
!! AATATAAAGAGAGAACAAAA+TGG - chr1_3:2654565-2654584 None:intergenic 20.0%
!! TATTTATGATGAATTTGTCA+TGG - chr1_3:2655132-2655151 None:intergenic 20.0%
!!! CAACTTTTCATTTCATTTTA+TGG + chr1_3:2655183-2655202 Msa0096910:intron 20.0%
!!! ATTTTATGGTAGAGAAAATA+AGG + chr1_3:2655197-2655216 Msa0096910:intron 20.0%
!! TGATGAAATAAAGAACAAAA+GGG - chr1_3:2654253-2654272 None:intergenic 20.0%
!! TTGATGAAATAAAGAACAAA+AGG - chr1_3:2654254-2654273 None:intergenic 20.0%
!! AATATAAAGAGAGAACAAAA+TGG - chr1_3:2654565-2654584 None:intergenic 20.0%
!! TATTTATGATGAATTTGTCA+TGG - chr1_3:2655132-2655151 None:intergenic 20.0%
!!! CAACTTTTCATTTCATTTTA+TGG + chr1_3:2655183-2655202 Msa0096910:intron 20.0%
!!! ATTTTATGGTAGAGAAAATA+AGG + chr1_3:2655197-2655216 Msa0096910:intron 20.0%
!!! AATCTCTTCATTGTTTTTGT+TGG - chr1_3:2653883-2653902 None:intergenic 25.0%
!! TCTCTCTTCTTTTTCTATTT+AGG + chr1_3:2654131-2654150 Msa0096910:CDS 25.0%
! ATATGTACTTGACTGATATT+GGG + chr1_3:2654648-2654667 Msa0096910:intron 25.0%
!! TATGGCCAATTTTTAAATTG+TGG + chr1_3:2654734-2654753 Msa0096910:intron 25.0%
! TTGGCTTCTTACAAAAAATT+CGG + chr1_3:2654870-2654889 Msa0096910:intron 25.0%
! ACATGTTACAATTACATGAA+TGG - chr1_3:2654980-2654999 None:intergenic 25.0%
!!! GTTTTGTTTTGATGTTTATC+AGG + chr1_3:2655245-2655264 Msa0096910:intron 25.0%
!!! AATCTCTTCATTGTTTTTGT+TGG - chr1_3:2653883-2653902 None:intergenic 25.0%
!! TCTCTCTTCTTTTTCTATTT+AGG + chr1_3:2654131-2654150 Msa0096910:CDS 25.0%
! ATATGTACTTGACTGATATT+GGG + chr1_3:2654648-2654667 Msa0096910:intron 25.0%
!! TATGGCCAATTTTTAAATTG+TGG + chr1_3:2654734-2654753 Msa0096910:intron 25.0%
! TTGGCTTCTTACAAAAAATT+CGG + chr1_3:2654870-2654889 Msa0096910:intron 25.0%
! ACATGTTACAATTACATGAA+TGG - chr1_3:2654980-2654999 None:intergenic 25.0%
!!! GTTTTGTTTTGATGTTTATC+AGG + chr1_3:2655245-2655264 Msa0096910:intron 25.0%
GAAGAGATTCAGAATTTCAA+TGG + chr1_3:2653894-2653913 Msa0096910:CDS 30.0%
TGAAGCAATAAAATTCCCTT+GGG - chr1_3:2654001-2654020 None:intergenic 30.0%
GAAATAAAGAACAAAAGGGA+AGG - chr1_3:2654249-2654268 None:intergenic 30.0%
CATATGTACTTGACTGATAT+TGG + chr1_3:2654647-2654666 Msa0096910:intron 30.0%
TAAAATGTTGCGATTGAATG+TGG + chr1_3:2654683-2654702 Msa0096910:intron 30.0%
AAAATGTTGCGATTGAATGT+GGG + chr1_3:2654684-2654703 Msa0096910:intron 30.0%
AGCGACCACAATTTAAAAAT+TGG - chr1_3:2654742-2654761 None:intergenic 30.0%
! AATTTTTAAATTGTGGTCGC+TGG + chr1_3:2654741-2654760 Msa0096910:intron 30.0%
! TCTCATTAGTGTCAATTTTG+TGG + chr1_3:2654841-2654860 Msa0096910:intron 30.0%
! CTCATTAGTGTCAATTTTGT+GGG + chr1_3:2654842-2654861 Msa0096910:intron 30.0%
TGGCTTCTTACAAAAAATTC+GGG + chr1_3:2654871-2654890 Msa0096910:intron 30.0%
GAAGAGATTCAGAATTTCAA+TGG + chr1_3:2653894-2653913 Msa0096910:CDS 30.0%
TGAAGCAATAAAATTCCCTT+GGG - chr1_3:2654001-2654020 None:intergenic 30.0%
GAAATAAAGAACAAAAGGGA+AGG - chr1_3:2654249-2654268 None:intergenic 30.0%
CATATGTACTTGACTGATAT+TGG + chr1_3:2654647-2654666 Msa0096910:intron 30.0%
TAAAATGTTGCGATTGAATG+TGG + chr1_3:2654683-2654702 Msa0096910:intron 30.0%
AAAATGTTGCGATTGAATGT+GGG + chr1_3:2654684-2654703 Msa0096910:intron 30.0%
AGCGACCACAATTTAAAAAT+TGG - chr1_3:2654742-2654761 None:intergenic 30.0%
! AATTTTTAAATTGTGGTCGC+TGG + chr1_3:2654741-2654760 Msa0096910:intron 30.0%
! TCTCATTAGTGTCAATTTTG+TGG + chr1_3:2654841-2654860 Msa0096910:intron 30.0%
! CTCATTAGTGTCAATTTTGT+GGG + chr1_3:2654842-2654861 Msa0096910:intron 30.0%
TGGCTTCTTACAAAAAATTC+GGG + chr1_3:2654871-2654890 Msa0096910:intron 30.0%
!! CAATGGTTCTGAGCTAATTA+AGG + chr1_3:2653911-2653930 Msa0096910:CDS 35.0%
GAGTAAGACGCAATTCAAAT+GGG - chr1_3:2653959-2653978 None:intergenic 35.0%
GTGAAGCAATAAAATTCCCT+TGG - chr1_3:2654002-2654021 None:intergenic 35.0%
! ACAGGATTTTGGATGCAAAA+GGG + chr1_3:2654056-2654075 Msa0096910:CDS 35.0%
CTATTTAGGAAATTCACCGA+TGG + chr1_3:2654145-2654164 Msa0096910:CDS 35.0%
AACACACGACAAACAACAAA+AGG - chr1_3:2654337-2654356 None:intergenic 35.0%
ATGGATCTAAAAGGTGAGTT+CGG + chr1_3:2654373-2654392 Msa0096910:CDS 35.0%
CATCAAAATCAGTGATGTTG+TGG - chr1_3:2654710-2654729 None:intergenic 35.0%
!!! CACTGATTTTGATGTCTGTA+TGG + chr1_3:2654716-2654735 Msa0096910:intron 35.0%
! GTCAATTTTGTGGGATAACT+TGG + chr1_3:2654851-2654870 Msa0096910:intron 35.0%
!! CAATGGTTCTGAGCTAATTA+AGG + chr1_3:2653911-2653930 Msa0096910:CDS 35.0%
GAGTAAGACGCAATTCAAAT+GGG - chr1_3:2653959-2653978 None:intergenic 35.0%
GTGAAGCAATAAAATTCCCT+TGG - chr1_3:2654002-2654021 None:intergenic 35.0%
! ACAGGATTTTGGATGCAAAA+GGG + chr1_3:2654056-2654075 Msa0096910:CDS 35.0%
CTATTTAGGAAATTCACCGA+TGG + chr1_3:2654145-2654164 Msa0096910:CDS 35.0%
AACACACGACAAACAACAAA+AGG - chr1_3:2654337-2654356 None:intergenic 35.0%
ATGGATCTAAAAGGTGAGTT+CGG + chr1_3:2654373-2654392 Msa0096910:CDS 35.0%
CATCAAAATCAGTGATGTTG+TGG - chr1_3:2654710-2654729 None:intergenic 35.0%
!!! CACTGATTTTGATGTCTGTA+TGG + chr1_3:2654716-2654735 Msa0096910:intron 35.0%
! GTCAATTTTGTGGGATAACT+TGG + chr1_3:2654851-2654870 Msa0096910:intron 35.0%
TAACAAGGAGCTCATCAGAA+GGG - chr1_3:2653848-2653867 None:intergenic 40.0%
TGGCAAGATAGTAGCTAACA+AGG - chr1_3:2653863-2653882 None:intergenic 40.0%
GCAATTCAAATGGGTCATAC+TGG - chr1_3:2653950-2653969 None:intergenic 40.0%
GGAGTAAGACGCAATTCAAA+TGG - chr1_3:2653960-2653979 None:intergenic 40.0%
! GGTACTGTAAGACAGGATTT+TGG + chr1_3:2654045-2654064 Msa0096910:intron 40.0%
! GACAGGATTTTGGATGCAAA+AGG + chr1_3:2654055-2654074 Msa0096910:CDS 40.0%
AAGCCTCAGGTATGACATTT+GGG + chr1_3:2654215-2654234 Msa0096910:intron 40.0%
TTTCCCAAATGTCATACCTG+AGG - chr1_3:2654221-2654240 None:intergenic 40.0%
AGGACGTGATTTGAAGAATC+TGG - chr1_3:2655088-2655107 None:intergenic 40.0%
TAACAAGGAGCTCATCAGAA+GGG - chr1_3:2653848-2653867 None:intergenic 40.0%
TGGCAAGATAGTAGCTAACA+AGG - chr1_3:2653863-2653882 None:intergenic 40.0%
GCAATTCAAATGGGTCATAC+TGG - chr1_3:2653950-2653969 None:intergenic 40.0%
GGAGTAAGACGCAATTCAAA+TGG - chr1_3:2653960-2653979 None:intergenic 40.0%
! GGTACTGTAAGACAGGATTT+TGG + chr1_3:2654045-2654064 Msa0096910:intron 40.0%
! GACAGGATTTTGGATGCAAA+AGG + chr1_3:2654055-2654074 Msa0096910:CDS 40.0%
AAGCCTCAGGTATGACATTT+GGG + chr1_3:2654215-2654234 Msa0096910:intron 40.0%
TTTCCCAAATGTCATACCTG+AGG - chr1_3:2654221-2654240 None:intergenic 40.0%
AGGACGTGATTTGAAGAATC+TGG - chr1_3:2655088-2655107 None:intergenic 40.0%
GAATTGTTTCCACCTGGAGT+TGG + chr1_3:2653816-2653835 Msa0096910:CDS 45.0%
CTAACAAGGAGCTCATCAGA+AGG - chr1_3:2653849-2653868 None:intergenic 45.0%
CAAATGGGTCATACTGGTAC+AGG - chr1_3:2653944-2653963 None:intergenic 45.0%
! CTTTTCCTTCTGTCACCCAA+GGG + chr1_3:2653983-2654002 Msa0096910:intron 45.0%
AAATTCCCTTGGGTGACAGA+AGG - chr1_3:2653991-2654010 None:intergenic 45.0%
AACCACGTTATCAGCTCCAA+CGG - chr1_3:2654099-2654118 None:intergenic 45.0%
CAAGCCTCAGGTATGACATT+TGG + chr1_3:2654214-2654233 Msa0096910:intron 45.0%
! CTTTTAGATCCATCCCTGGT+GGG - chr1_3:2654366-2654385 None:intergenic 45.0%
! TCACCTTTTAGATCCATCCC+TGG - chr1_3:2654370-2654389 None:intergenic 45.0%
TGATGTCGCTCAGAAACCTA+TGG + chr1_3:2654441-2654460 Msa0096910:CDS 45.0%
GATGTCGCTCAGAAACCTAT+GGG + chr1_3:2654442-2654461 Msa0096910:CDS 45.0%
ATGTCGCTCAGAAACCTATG+GGG + chr1_3:2654443-2654462 Msa0096910:CDS 45.0%
AGGTGATGCATCGTATTTGC+AGG + chr1_3:2654528-2654547 Msa0096910:CDS 45.0%
TGACTGTGTTGTCTTCCACA+TGG + chr1_3:2655287-2655306 Msa0096910:CDS 45.0%
CCACATGGAAGCTATGATAC+CGG + chr1_3:2655302-2655321 Msa0096910:CDS 45.0%
GAATTGTTTCCACCTGGAGT+TGG + chr1_3:2653816-2653835 Msa0096910:CDS 45.0%
CTAACAAGGAGCTCATCAGA+AGG - chr1_3:2653849-2653868 None:intergenic 45.0%
CAAATGGGTCATACTGGTAC+AGG - chr1_3:2653944-2653963 None:intergenic 45.0%
! CTTTTCCTTCTGTCACCCAA+GGG + chr1_3:2653983-2654002 Msa0096910:intron 45.0%
AAATTCCCTTGGGTGACAGA+AGG - chr1_3:2653991-2654010 None:intergenic 45.0%
AACCACGTTATCAGCTCCAA+CGG - chr1_3:2654099-2654118 None:intergenic 45.0%
CAAGCCTCAGGTATGACATT+TGG + chr1_3:2654214-2654233 Msa0096910:intron 45.0%
! CTTTTAGATCCATCCCTGGT+GGG - chr1_3:2654366-2654385 None:intergenic 45.0%
! TCACCTTTTAGATCCATCCC+TGG - chr1_3:2654370-2654389 None:intergenic 45.0%
TGATGTCGCTCAGAAACCTA+TGG + chr1_3:2654441-2654460 Msa0096910:CDS 45.0%
GATGTCGCTCAGAAACCTAT+GGG + chr1_3:2654442-2654461 Msa0096910:CDS 45.0%
ATGTCGCTCAGAAACCTATG+GGG + chr1_3:2654443-2654462 Msa0096910:CDS 45.0%
AGGTGATGCATCGTATTTGC+AGG + chr1_3:2654528-2654547 Msa0096910:CDS 45.0%
TGACTGTGTTGTCTTCCACA+TGG + chr1_3:2655287-2655306 Msa0096910:CDS 45.0%
CCACATGGAAGCTATGATAC+CGG + chr1_3:2655302-2655321 Msa0096910:CDS 45.0%
!! AAAAAAATTTAAAGAAAAAA+AGG - chr1_3:2655108-2655127 None:intergenic 5.0%
!! AAAAAAATTTAAAGAAAAAA+AGG - chr1_3:2655108-2655127 None:intergenic 5.0%
GGGTGACAGAAGGAAAAGGA+TGG - chr1_3:2653981-2654000 None:intergenic 50.0%
CCTTGGGTGACAGAAGGAAA+AGG - chr1_3:2653985-2654004 None:intergenic 50.0%
! CCTTTTCCTTCTGTCACCCA+AGG + chr1_3:2653982-2654001 Msa0096910:intron 50.0%
!! CTGCCAAAGTTGATGAGCAG+AGG + chr1_3:2654021-2654040 Msa0096910:intron 50.0%
CCTCCTCTGCTCATCAACTT+TGG - chr1_3:2654027-2654046 None:intergenic 50.0%
!! CCAAAGTTGATGAGCAGAGG+AGG + chr1_3:2654024-2654043 Msa0096910:intron 50.0%
!! CAGAGGAGGTACTGTAAGAC+AGG + chr1_3:2654038-2654057 Msa0096910:intron 50.0%
GCTGTTCGAGACATTACCGT+TGG + chr1_3:2654080-2654099 Msa0096910:CDS 50.0%
! TACCGTTGGAGCTGATAACG+TGG + chr1_3:2654094-2654113 Msa0096910:CDS 50.0%
! GGAGCTGATAACGTGGTTCT+AGG + chr1_3:2654101-2654120 Msa0096910:CDS 50.0%
!!! CTGGTTTTGACAGCGTCCAT+CGG - chr1_3:2654164-2654183 None:intergenic 50.0%
GTACTCGTACATAGTCCAGC+TGG - chr1_3:2654183-2654202 None:intergenic 50.0%
CACTCTTGCTAACAAGCCTC+AGG + chr1_3:2654202-2654221 Msa0096910:CDS 50.0%
! CCTTTTAGATCCATCCCTGG+TGG - chr1_3:2654367-2654386 None:intergenic 50.0%
CCACCAGGGATGGATCTAAA+AGG + chr1_3:2654364-2654383 Msa0096910:CDS 50.0%
! GCCTTGACGGATGTTGAAGA+AGG + chr1_3:2654397-2654416 Msa0096910:CDS 50.0%
GCCTTCTTCAACATCCGTCA+AGG - chr1_3:2654401-2654420 None:intergenic 50.0%
TGTCGCTCAGAAACCTATGG+GGG + chr1_3:2654444-2654463 Msa0096910:CDS 50.0%
! GCTAGTGCTGCTATTGGTGT+TGG + chr1_3:2654472-2654491 Msa0096910:CDS 50.0%
CCGGTATCATAGCTTCCATG+TGG - chr1_3:2655305-2655324 None:intergenic 50.0%
AGTGCATGCTTAAGCTGCTC+CGG - chr1_3:2655324-2655343 None:intergenic 50.0%
GCAGCTTAAGCATGCACTTC+TGG + chr1_3:2655326-2655345 Msa0096910:CDS 50.0%
GGGTGACAGAAGGAAAAGGA+TGG - chr1_3:2653981-2654000 None:intergenic 50.0%
CCTTGGGTGACAGAAGGAAA+AGG - chr1_3:2653985-2654004 None:intergenic 50.0%
! CCTTTTCCTTCTGTCACCCA+AGG + chr1_3:2653982-2654001 Msa0096910:intron 50.0%
!! CTGCCAAAGTTGATGAGCAG+AGG + chr1_3:2654021-2654040 Msa0096910:intron 50.0%
CCTCCTCTGCTCATCAACTT+TGG - chr1_3:2654027-2654046 None:intergenic 50.0%
!! CCAAAGTTGATGAGCAGAGG+AGG + chr1_3:2654024-2654043 Msa0096910:intron 50.0%
!! CAGAGGAGGTACTGTAAGAC+AGG + chr1_3:2654038-2654057 Msa0096910:intron 50.0%
GCTGTTCGAGACATTACCGT+TGG + chr1_3:2654080-2654099 Msa0096910:CDS 50.0%
! TACCGTTGGAGCTGATAACG+TGG + chr1_3:2654094-2654113 Msa0096910:CDS 50.0%
! GGAGCTGATAACGTGGTTCT+AGG + chr1_3:2654101-2654120 Msa0096910:CDS 50.0%
!!! CTGGTTTTGACAGCGTCCAT+CGG - chr1_3:2654164-2654183 None:intergenic 50.0%
GTACTCGTACATAGTCCAGC+TGG - chr1_3:2654183-2654202 None:intergenic 50.0%
CACTCTTGCTAACAAGCCTC+AGG + chr1_3:2654202-2654221 Msa0096910:CDS 50.0%
! CCTTTTAGATCCATCCCTGG+TGG - chr1_3:2654367-2654386 None:intergenic 50.0%
CCACCAGGGATGGATCTAAA+AGG + chr1_3:2654364-2654383 Msa0096910:CDS 50.0%
! GCCTTGACGGATGTTGAAGA+AGG + chr1_3:2654397-2654416 Msa0096910:CDS 50.0%
GCCTTCTTCAACATCCGTCA+AGG - chr1_3:2654401-2654420 None:intergenic 50.0%
TGTCGCTCAGAAACCTATGG+GGG + chr1_3:2654444-2654463 Msa0096910:CDS 50.0%
! GCTAGTGCTGCTATTGGTGT+TGG + chr1_3:2654472-2654491 Msa0096910:CDS 50.0%
CCGGTATCATAGCTTCCATG+TGG - chr1_3:2655305-2655324 None:intergenic 50.0%
AGTGCATGCTTAAGCTGCTC+CGG - chr1_3:2655324-2655343 None:intergenic 50.0%
GCAGCTTAAGCATGCACTTC+TGG + chr1_3:2655326-2655345 Msa0096910:CDS 50.0%
TGGACGCTGTCAAAACCAGC+TGG + chr1_3:2654165-2654184 Msa0096910:CDS 55.0%
GTGTGTTTGACTGCCCACCA+GGG + chr1_3:2654350-2654369 Msa0096910:CDS 55.0%
AGGTGAGTTCGGAGCCTTGA+CGG + chr1_3:2654384-2654403 Msa0096910:CDS 55.0%
GTTGAAGAAGGCGATGCTGC+TGG + chr1_3:2654409-2654428 Msa0096910:CDS 55.0%
! GCTGGTGCTAGTGCTGCTAT+TGG + chr1_3:2654466-2654485 Msa0096910:CDS 55.0%
!! GTTGGTTCTGCTGCTGATGC+TGG + chr1_3:2654490-2654509 Msa0096910:CDS 55.0%
TGGACGCTGTCAAAACCAGC+TGG + chr1_3:2654165-2654184 Msa0096910:CDS 55.0%
GTGTGTTTGACTGCCCACCA+GGG + chr1_3:2654350-2654369 Msa0096910:CDS 55.0%
AGGTGAGTTCGGAGCCTTGA+CGG + chr1_3:2654384-2654403 Msa0096910:CDS 55.0%
GTTGAAGAAGGCGATGCTGC+TGG + chr1_3:2654409-2654428 Msa0096910:CDS 55.0%
! GCTGGTGCTAGTGCTGCTAT+TGG + chr1_3:2654466-2654485 Msa0096910:CDS 55.0%
!! GTTGGTTCTGCTGCTGATGC+TGG + chr1_3:2654490-2654509 Msa0096910:CDS 55.0%
GAAGGGACGAAGCCAACTCC+AGG - chr1_3:2653831-2653850 None:intergenic 60.0%
CGTGTGTTTGACTGCCCACC+AGG + chr1_3:2654349-2654368 Msa0096910:CDS 60.0%
GTTTGACTGCCCACCAGGGA+TGG + chr1_3:2654354-2654373 Msa0096910:CDS 60.0%
GCTCAGAAACCTATGGGGGC+TGG + chr1_3:2654448-2654467 Msa0096910:CDS 60.0%
GAAGGGACGAAGCCAACTCC+AGG - chr1_3:2653831-2653850 None:intergenic 60.0%
CGTGTGTTTGACTGCCCACC+AGG + chr1_3:2654349-2654368 Msa0096910:CDS 60.0%
GTTTGACTGCCCACCAGGGA+TGG + chr1_3:2654354-2654373 Msa0096910:CDS 60.0%
GCTCAGAAACCTATGGGGGC+TGG + chr1_3:2654448-2654467 Msa0096910:CDS 60.0%
GGGACGAAGCCAACTCCAGG+TGG - chr1_3:2653828-2653847 None:intergenic 65.0%
GCACTAGCACCAGCCCCCAT+AGG - chr1_3:2654460-2654479 None:intergenic 65.0%
! GCTGGTCGTGCTGCTGAGCA+AGG + chr1_3:2654508-2654527 Msa0096910:CDS 65.0%
GGGACGAAGCCAACTCCAGG+TGG - chr1_3:2653828-2653847 None:intergenic 65.0%
GCACTAGCACCAGCCCCCAT+AGG - chr1_3:2654460-2654479 None:intergenic 65.0%
! GCTGGTCGTGCTGCTGAGCA+AGG + chr1_3:2654508-2654527 Msa0096910:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 2653813 2655383 2653813 ID=Msa0096910;Name=Msa0096910
chr1_3 mRNA 2653813 2655383 2653813 ID=Msa0096910-mRNA-1;Parent=Msa0096910;Name=Msa0096910-mRNA-1;_AED=0.28;_eAED=0.35;_QI=0|0|0|0.5|1|1|4|0|216
chr1_3 exon 2653813 2653949 2653813 ID=Msa0096910-mRNA-1:exon:688;Parent=Msa0096910-mRNA-1
chr1_3 exon 2654046 2654223 2654046 ID=Msa0096910-mRNA-1:exon:689;Parent=Msa0096910-mRNA-1
chr1_3 exon 2654331 2654549 2654331 ID=Msa0096910-mRNA-1:exon:690;Parent=Msa0096910-mRNA-1
chr1_3 exon 2655267 2655383 2655267 ID=Msa0096910-mRNA-1:exon:691;Parent=Msa0096910-mRNA-1
chr1_3 CDS 2653813 2653949 2653813 ID=Msa0096910-mRNA-1:cds;Parent=Msa0096910-mRNA-1
chr1_3 CDS 2654046 2654223 2654046 ID=Msa0096910-mRNA-1:cds;Parent=Msa0096910-mRNA-1
chr1_3 CDS 2654331 2654549 2654331 ID=Msa0096910-mRNA-1:cds;Parent=Msa0096910-mRNA-1
chr1_3 CDS 2655267 2655383 2655267 ID=Msa0096910-mRNA-1:cds;Parent=Msa0096910-mRNA-1
Gene Sequence

>Msa0096910

ATGGAATTGTTTCCACCTGGAGTTGGCTTCGTCCCTTCTGATGAGCTCCTTGTTAGCTACTATCTTGCCAACAAAAACAATGAAGAGATTCAGAATTTCAATGGTTCTGAGCTAATTAAGGAGCTGAACCTGTACCAGTACTGTAAGACAGGATTTTGGATGCAAAAGGGAGCTGTTCGAGACATTACCGTTGGAGCTGATAACGTGGTTCTAGGAAAGAAGTCTCTCTTCTTTTTCTATTTAGGAAATTCACCGATGGACGCTGTCAAAACCAGCTGGACTATGTACGAGTACACTCTTGCTAACAAGCCTCAGGCTCCTTTTGTTGTTTGTCGTGTGTTTGACTGCCCACCAGGGATGGATCTAAAAGGTGAGTTCGGAGCCTTGACGGATGTTGAAGAAGGCGATGCTGCTGGCGATGACTCTGATGTCGCTCAGAAACCTATGGGGGCTGGTGCTAGTGCTGCTATTGGTGTTGGTTCTGCTGCTGATGCTGGTCGTGCTGCTGAGCAAGGTGATGCATCGTATTTGCAGGCGAGTTATGCTGCATTTCTTGACTGTGTTGTCTTCCACATGGAAGCTATGATACCGGAGCAGCTTAAGCATGCACTTCTGGACGAAGATTTTCTTGAACTCGCTGATTTTGAATAA
Protein sequence

>Msa0096910

MELFPPGVGFVPSDELLVSYYLANKNNEEIQNFNGSELIKELNLYQYCKTGFWMQKGAVRDITVGADNVVLGKKSLFFFYLGNSPMDAVKTSWTMYEYTLANKPQAPFVVCRVFDCPPGMDLKGEFGALTDVEEGDAAGDDSDVAQKPMGAGASAAIGVGSAADAGRAAEQGDASYLQASYAAFLDCVVFHMEAMIPEQLKHALLDEDFLELADFE*